CN106754927A - 核酸在提高细胞巨核分化效率中的用途 - Google Patents
核酸在提高细胞巨核分化效率中的用途 Download PDFInfo
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- CN106754927A CN106754927A CN201611217257.8A CN201611217257A CN106754927A CN 106754927 A CN106754927 A CN 106754927A CN 201611217257 A CN201611217257 A CN 201611217257A CN 106754927 A CN106754927 A CN 106754927A
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- C12N2310/00—Structure or type of the nucleic acid
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Abstract
Description
组成成分 | 加入量 |
5×miScriptHiFlex Buffer | 2μl |
10×miScriptNucleics Mix | 1μl |
miScriptReverseTranscriptase Mix | 1μl |
miRNA模板 | 800ng |
Nuclease-free Wate | 总体积补至10μl |
组成成分 | 加入量 |
All-in-oneq-PCRMix | 10μl |
H2O | 8μl |
cDNA | 1μl |
miRNA引物 | 0.5μl |
U3 | 0.5μl |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201611217257.8A CN106754927B (zh) | 2016-12-26 | 2016-12-26 | 核酸在提高细胞巨核分化效率中的用途 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201611217257.8A CN106754927B (zh) | 2016-12-26 | 2016-12-26 | 核酸在提高细胞巨核分化效率中的用途 |
Publications (2)
Publication Number | Publication Date |
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CN106754927A true CN106754927A (zh) | 2017-05-31 |
CN106754927B CN106754927B (zh) | 2019-10-08 |
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CN201611217257.8A Active CN106754927B (zh) | 2016-12-26 | 2016-12-26 | 核酸在提高细胞巨核分化效率中的用途 |
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CN (1) | CN106754927B (zh) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101368180A (zh) * | 2008-10-15 | 2009-02-18 | 中国科学院生物物理研究所 | 一类能将间充质干细胞分化为造血细胞的小rna分子及其作用靶点 |
CN101448958A (zh) * | 2006-03-20 | 2009-06-03 | 俄亥俄州立大学研究基金会 | 人巨核细胞生成期间的微小rna指纹 |
-
2016
- 2016-12-26 CN CN201611217257.8A patent/CN106754927B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101448958A (zh) * | 2006-03-20 | 2009-06-03 | 俄亥俄州立大学研究基金会 | 人巨核细胞生成期间的微小rna指纹 |
CN101368180A (zh) * | 2008-10-15 | 2009-02-18 | 中国科学院生物物理研究所 | 一类能将间充质干细胞分化为造血细胞的小rna分子及其作用靶点 |
Non-Patent Citations (4)
Title |
---|
EMMRICH S等: "miRNAs can increase the efficiency of ex vivo platelet generation.", 《ANNALS OF HEMATOLOGY》 * |
KILPINEN L等: "Expansion induced microRNA changes in bone marrow mesenchymal stromal cells reveals interplay between immune regulation and cell cycle.", 《AGING (ALBANY NY)》 * |
LU J等: "MicroRNA-mediated control of cell fate in megakaryocyte-erythrocyte progenitors.", 《DEVELOPMENTAL CELL》 * |
SIM X等: "Understanding platelet generation from megakaryocytes: implications for in vitro-derived platelets.", 《BLOOD》 * |
Also Published As
Publication number | Publication date |
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CN106754927B (zh) | 2019-10-08 |
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Inventor after: Xie Xiaoyan Inventor after: Qu Mingyi Inventor after: Pei Xuetao Inventor after: Yue Wen Inventor after: Fang Fang Inventor after: Chen Lin Inventor after: He Lijuan Inventor after: Zhang Bowen Inventor before: Pei Xuetao Inventor before: Xie Xiaoyan Inventor before: Qu Mingyi Inventor before: Yue Wen Inventor before: Fang Fang Inventor before: Chen Lin Inventor before: He Lijuan Inventor before: Zhang Bowen |