CN106754927B - 核酸在提高细胞巨核分化效率中的用途 - Google Patents
核酸在提高细胞巨核分化效率中的用途 Download PDFInfo
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- CN106754927B CN106754927B CN201611217257.8A CN201611217257A CN106754927B CN 106754927 B CN106754927 B CN 106754927B CN 201611217257 A CN201611217257 A CN 201611217257A CN 106754927 B CN106754927 B CN 106754927B
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- C12N5/0644—Platelets; Megakaryocytes
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- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/141—MicroRNAs, miRNAs
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- C12N2510/00—Genetically modified cells
Abstract
Description
组成成分 | 加入量 |
5×miScriptHiFlex Buffer | 2μl |
10×miScriptNucleics Mix | 1μl |
miScriptReverseTranscriptase Mix | 1μl |
miRNA模板 | 800ng |
Nuclease-free Wate | 总体积补至10μl |
组成成分 | 加入量 |
All-in-oneq-PCRMix | 10μl |
H<sub>2</sub>O | 8μl |
cDNA | 1μl |
miRNA引物 | 0.5μl |
U3 | 0.5μl |
Claims (13)
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CN201611217257.8A CN106754927B (zh) | 2016-12-26 | 2016-12-26 | 核酸在提高细胞巨核分化效率中的用途 |
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CN201611217257.8A CN106754927B (zh) | 2016-12-26 | 2016-12-26 | 核酸在提高细胞巨核分化效率中的用途 |
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CN106754927A CN106754927A (zh) | 2017-05-31 |
CN106754927B true CN106754927B (zh) | 2019-10-08 |
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Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
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EP2371971B1 (en) * | 2006-03-20 | 2013-11-27 | The Ohio State University Research Foundation | Microrna fingerprints during human megakaryocytopoiesis |
CN101368180A (zh) * | 2008-10-15 | 2009-02-18 | 中国科学院生物物理研究所 | 一类能将间充质干细胞分化为造血细胞的小rna分子及其作用靶点 |
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Inventor after: Xie Xiaoyan Inventor after: Qu Mingyi Inventor after: Pei Xuetao Inventor after: Yue Wen Inventor after: Fang Fang Inventor after: Chen Lin Inventor after: He Lijuan Inventor after: Zhang Bowen Inventor before: Pei Xuetao Inventor before: Xie Xiaoyan Inventor before: Qu Mingyi Inventor before: Yue Wen Inventor before: Fang Fang Inventor before: Chen Lin Inventor before: He Lijuan Inventor before: Zhang Bowen |
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