CN106726868B - A kind of preparation method for the China rose anthocyanidin having whitening capability - Google Patents
A kind of preparation method for the China rose anthocyanidin having whitening capability Download PDFInfo
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- CN106726868B CN106726868B CN201611026841.5A CN201611026841A CN106726868B CN 106726868 B CN106726868 B CN 106726868B CN 201611026841 A CN201611026841 A CN 201611026841A CN 106726868 B CN106726868 B CN 106726868B
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- anthocyanidin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The invention discloses a kind of preparation methods of China rose anthocyanidin for having whitening capability, new fresh China rose is distilled with steam distillation, the ratio of water and flower is 4.5-5.5:1, water vapour after distillation is through cooling, obtain coolant liquid, when the coolant liquid weight distilled out is that 3-4 times of Chinese rose petal weight is added, that is, stop distillation；After steam distillation, China rose is boiled 0.5-1.5 hours jointly and is filtered in distiller bottom China rose Liquid Residue and distiller, filtrate removes highly polar ingredient by DIAION HP-20 tubing string, it is concentrated in vacuo and drains after collection efflux, it is freeze-dried up to Chinese rose anthocyanidin again, set -20 DEG C of ice storage hiding.Product of the present invention can inhibit melanocyte and generate melanin.
Description
Technical field
The present invention relates to cosmetic material fields, more particularly to a kind of China rose anthocyanidin preparation side for having whitening capability
Method.
Background technique
China rose (scientific name: Rosa chinensis Jacq.): referred to as spending middle queen, also known as " Chinese rose ", be it is evergreen,
Half evergreen short shrub, four Ji Kaihua ﹐ are generally red color ﹐ or pink colour, occasionally have white and Huang color ﹐ to can be used as ornamental plant, can also
As medicinal plant, also known as Chinese rose.There are three natural mutation, modern distance education system flower pattern multiplicity, have single-lobe and polyphyll, there are also Gao Xinjuan
The grace flower pattern such as side；Its is beautiful in colour, abundant, not only there is that red, powder is yellow, the monochromes such as white, and there are also the kinds such as colour mixture, silver-colored side；It is most
Kind has fragrance.Chinese rose it is various in style, have nearly ten thousand kinds in the world, China also has thousand kinds or more.
Natural florescence August is to April next year, and flower is at large size, from inside to outside, is in divergence form, has strong fragrance, can be widely used for
Horticultural gardening and cut-flower.Chinese rose it is adaptable, resist cold, it is cold-resistant, though ground plant, potting, suitable for beautifying the yard, decorateeing
Gardens arrange flower bed, plant colored hedge, flower stand, and Chinese rose cultivation is easy, and can make cut-flower, for doing bouquet and the various gailys decorated basket, China rose
Piece extractable essence, and can be used as medicine.Also there is preferable antimycotic and collaboration overriding resistance fungi activity.
Summary of the invention
The purpose of the present invention is to provide a kind of China rose anthocyanidin preparation methods for having whitening capability.
Technical solution provided by the invention is as follows:
An object of the present invention is to provide a kind of China rose anthocyanidin preparation method for having whitening capability, including such as
Lower step: new fresh China rose is distilled with steam distillation, water and the ratio spent are 4.5-5.5:1, the water vapour after distilling
Through cooling, coolant liquid is obtained, when the coolant liquid weight distilled out is that 3-4 times of Chinese rose petal weight is added, that is, stops distillation；With
After steam distillation, China rose is boiled 0.5-1.5 hours jointly and is filtered in distiller bottom China rose Liquid Residue and distiller, filter
Liquid removes highly polar ingredient by DIAION HP-20 tubing string, collects be concentrated in vacuo after efflux and drain, then freeze-dried is
Chinese rose anthocyanidin is obtained, -20 DEG C of ice storage hiding are set.
Preferably, distilling new fresh China rose with steam distillation, water and the ratio spent are 5:1, with steam distillation
Coolant liquid through cooling gained stops steaming when the coolant liquid weight distilled out is that 3-4 times of Chinese rose petal weight is added
It evaporates；After steam distillation, China rose is boiled 1 hour jointly and is filtered in distiller bottom China rose Liquid Residue and distiller, filter
Liquid removes highly polar ingredient by the DIAION HP-20 tubing string to configure completion, is concentrated in vacuo and drains after collection efflux, then
Up to Chinese rose anthocyanidin, -20 DEG C of ice storage hiding are set within freeze-dried 24 hours.
It is still a further object of the present invention is to provide a kind of purposes of China rose anthocyanidin, it is used to inhibit DPPH free radical.
It is still a further object of the present invention is to provide a kind of purposes of China rose anthocyanidin, it is used to inhibit to inhibit tyraminase
Activity.
It is still a further object of the present invention is to provide a kind of purposes of China rose anthocyanidin, for inhibiting melanocyte raw
At melanin.
Seen from the above description, the present invention provides a kind of China rose anthocyanidin preparation method for having whitening capability, this hairs
The China rose anthocyanidin of bright preparation can be used for inhibiting DPPH free radical, inhibit tyrasamine enzymatic activity or inhibit melanocyte
Generate melanin.And to human skin without stimulate the reactions such as redness, edema, papule, bubbles.
Detailed description of the invention
Fig. 1 is that the season anthocyanidin of the embodiment of the present invention 5 inhibits the experimental result picture of DPPH free radical；Wherein abscissa is dense
It spends (ppm), ordinate is inhibiting rate (%)；
Fig. 2 is 8 patch tests result of the embodiment of the present invention；
Specific embodiment
Embodiment 1
Chinese rose anthosin dew, Chinese rose anthocyanidin production technology:
1. distilling new fresh China rose (selecting bright red system's China rose) with steam distillation, water and the ratio spent are 5:1, with
Water vapour after distillation obtains coolant liquid through cooling, when the coolant liquid weight distilled out is that 3 times of Chinese rose petal weight are added,
Stop distillation, this is Chinese rose essence dew, containing natural Chinese rose fragrance, can be used as natural Chinese rose water and is used.
2. it is small that China rose Liquid Residue and the China rose in distiller of distiller bottom boil 1 jointly after steam distillation
When filter, filtrate is by removing highly polar ingredient to configure the DIAION HP-20 tubing string completed, and vacuum is dense after collecting efflux
Contracting is drained, then freeze-dried 24 hours up to Chinese rose anthocyanidin, set -20 DEG C of ice storage hiding.
Total Flavonoid Content of 2 Chinese rose anthocyanidin of embodiment measures
Flavonoids can form stable yellow misfit object under alkaline condition with aluminium chloride, and in wavelength 415nm
Under have extinction, when color is deeper, Flavonoid Content is higher.Take one 96 porose discs that sample or various concentration standard items 20mL is added again
60mL ethyl alcohol (95%), 10% aluminium chloride of 4mL (aluminum chloride), 4mL 1M potassium acetate is sequentially added
(potassium acetate) and 112mL deionized water are protected from light 30 minutes, at room temperature by ELISA reader in wave
Long 415nm surveys its light absorption value.According to Flavonoid Content number in light absorption value discriminating test sample, previously system is substituted into using its light absorption value
Quercetin (quercetin) standard curve of work makes the concentration expression relative to Quercetin.Finally, conversing every gram of sample
In contain how many milligram Flavonoid Content.
Total Flavonoid Content measurement result of Chinese rose anthocyanidin
| Sample | Total Flavonoid Content (mg Quercetin eq./g) |
| Chinese rose anthocyanidin extract | 26.6±2.0 |
The total phenol content of 3 Chinese rose anthocyanidin of embodiment measures
The measurement of total phenol content for standard items, makes the gallic acid of various concentration with gallic acid (gallic acid)
The calibration curve of light absorption value surveys the light absorption value of 750nm using ELISA, and using light absorption value as the longitudinal axis, gallic acid concentration of standard solution is
Horizontal axis draws standard curve, and calculates quantitative linearity regression formula.Light absorption value measured by sample is added by gallic acid calibration curve
After conversion, the Determination of Polyphenols that sample to be tested can be obtained is equivalent to the content of how many gallic acids, the amount of gallic acid
Higher, the antioxidant activity of representation compound is higher.
Gallic acid standard curve making:
(1) the gallic acid aqueous solution that compound concentration is 12.5,25,50,100,200 and 400 μ g/mL respectively.
(2) standard aqueous solution of the 50 above-mentioned concentration of μ L is respectively taken, 50 μ L forint phenol reagent (Folin-Ciocalteu's are added
Phenol reagent), after evenly mixing in being stored at room temperature 20 minutes.
(3) adding 100 μ L concentration is 20%Na2CO3Aqueous solution, mixing be placed on room temperature reaction 10 minutes, measurement in
The light absorption value (working as blank control with water) of 750nm.
It (4) is the longitudinal axis using light absorption value, gallic acid concentration of standard solution is that horizontal axis draws standard curve, and calculates quantitative
Linear regression formula.
The total phenol content of Chinese rose anthocyanidin measures
| Sample | Total phenol content (mg Gallic acid eq./g) |
| Chinese rose anthocyanidin extract | 102.2±1.4 |
4 Chinese rose anthocyanidin Cytotoxicity evaluation of embodiment (Colorimetric MTT assay)
SCC25 cell (human tau neck squamous cancer cell line) and HaCaT (human skin horn cell) is selected to assess the moon
Season 72 hour cell toxicity test of anthocyanidin, Chinese rose anthocyanidin 100mg/mL slightly has a little toxicity to SCC25 cell as the result is shown,
But cell survival degree is still above 70% or more；Anthocyanidin, which does not have toxicity, to be shown to HaCaT (human skin horn cell).
The assessment of 5 Chinese rose anthocyanidin of embodiment inhibition DPPH free radical
Method: being protected from light 30 minutes after taking 99 μ L DPPH ethanol solutions (100 μ L) to mix the extract of 1 μ L, finally in
Light absorption value (BioTek, SynergyTM2, USA) is measured under wavelength 517nm.Compared with control group Vitamin C.
For anthocyanidin when for 100ppm concentration, display can reach 94.2% rejection ability as the result is shown, show China rose
Blueness is known as the efficiency of very excellent inhibition DPPH free radical.
| Sample ID | Inhibiting rate (%) |
| Vit C (control group) | 97.7±0.9 |
| Anthocyanidin | 94.2±0.2 |
6 Chinese rose anthocyanidin of embodiment inhibits tyraminase Activity Assessment
Under concentration 100ppm, Chinese rose anthocyanidin shows the inhibition Tyrosinase effect more than 50%.
| Sample ID | Inhibiting rate (%) |
| Vit C (control group) | 98.2±1.0 |
| Anthocyanidin | 58.6±2.2 |
7 Chinese rose anthocyanidin of embodiment inhibits the Activity Assessment of melanocyte (B16) generation melanin
By B16 cell, with 1 × 105The density of/mL is inoculated in cultivated 24 hours in 96 orifice plates after, by extract and control
Group vitamin C is mixed in taking after reaction 72 hours without phenol red (phenol red free) culture solution containing 100nM α-MSH
100 μ L of supernatant measures light absorption value (BioTek, SynergyTM2, USA) in 96 orifice plates, in 405nm wavelength.Calculate extract
With the light absorption value of vitamin C itself, conversion extract and vitamin C act on inhibition Melanin productions ability after cell.
Chinese rose anthocyanidin had inhibitory effect under 100ppm concentration, in height under 72 hours action time as the result is shown
The dosage 500ppm of concentration can have found the effect of significantly inhibiting B16 cell to generate melanin.
Embodiment August season anthocyanidin skin irritation tests (patch test)
Assessment result: Chinese rose anthocyanidin skin irritation test result is shown in observation in 24,48,72 hours and pierces without skin
Swash property reaction.
Experimental method: according to the regulation of International Contact Dermatitis study group (ICDRG), is observed in a manner of vision,
By obtained sample configuration at 10% aqueous solution, then 0.1ml is taken to be placed in the plastics that 1.0 × 1.0cm encloses gauze with pipette
Disk separately takes 0.1ml filtering secondary water as blank control group, is attached to Medial upper arm, is fixed with breathable adhesive tape, avoids having a bath and do
It is wet, every 24 hours gauzes more renewed, and the tested substance of addition 0.1ml and blank group again, it observes 72 hours.Observation skin has
Without stimulate the reactions such as redness, edema, papule, bubbles.(experiment person-time is 10 people).
Claims (3)
1. a kind of China rose anthocyanidin preparation method for having whitening capability, it is characterised in that: distilled with steam distillation fresh
The ratio of China rose, water and flower is 4.5-5.5:1, and the water vapour after distilling obtains coolant liquid through cooling, when the cooling distilled out
Liquid weight is when 3-4 times of Chinese rose petal weight is added, that is, to stop distillation；After steam distillation, distiller bottom China rose
China rose is boiled 0.5-1.5 hours jointly and is filtered in Liquid Residue and distiller, and filtrate removes high pole by DIAIONHP-20 tubing string
Property ingredient, be concentrated in vacuo and drains after collecting efflux, then is freeze-dried up to Chinese rose anthocyanidin, set -20 DEG C of ice storages and hide.
2. a kind of China rose anthocyanidin preparation method for having whitening capability, it is characterised in that: the coolant liquid distilled out is Chinese rose essence
Dew, containing natural Chinese rose fragrance, uses as natural Chinese rose water.
3. the purposes of China rose anthocyanidin is used to prepare the preparation for inhibiting melanocyte to generate melanin, wherein the moon
Season, flower anthocyanidin was prepared using method of claim 1, and the use concentration of China rose anthocyanidin is 100-500ppm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611026841.5A CN106726868B (en) | 2016-11-22 | 2016-11-22 | A kind of preparation method for the China rose anthocyanidin having whitening capability |
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|---|---|---|---|
| CN201611026841.5A CN106726868B (en) | 2016-11-22 | 2016-11-22 | A kind of preparation method for the China rose anthocyanidin having whitening capability |
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| Publication Number | Publication Date |
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| CN106726868A CN106726868A (en) | 2017-05-31 |
| CN106726868B true CN106726868B (en) | 2019-10-29 |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1511583A (en) * | 2002-12-28 | 2004-07-14 | 石家庄制药集团制药技术开发有限公司 | Chinese rose extract and its preparing method and use |
| CN101311225A (en) * | 2007-05-26 | 2008-11-26 | 王晓明 | Process for abstracting kohlrabi pigment |
| CN102048662A (en) * | 2009-10-30 | 2011-05-11 | 爱普香料集团股份有限公司 | Method for manufacturing rose water containing natural rose pigment |
| CN102838884A (en) * | 2012-01-18 | 2012-12-26 | 天津天康源生物技术有限公司 | Prevention method of anthocyanin pigment crystal precipitate |
| CN103044953A (en) * | 2011-10-13 | 2013-04-17 | 黎嘉纯 | Extraction process for Chinese rose pigment |
| CN105106047A (en) * | 2015-08-07 | 2015-12-02 | 刘学勤 | Water-replenishing and skin-tendering mask |
| CN105693678A (en) * | 2016-01-15 | 2016-06-22 | 甘肃寿鹿山药业有限公司 | Method for extracting procyanidine from grape seeds |
-
2016
- 2016-11-22 CN CN201611026841.5A patent/CN106726868B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1511583A (en) * | 2002-12-28 | 2004-07-14 | 石家庄制药集团制药技术开发有限公司 | Chinese rose extract and its preparing method and use |
| CN101311225A (en) * | 2007-05-26 | 2008-11-26 | 王晓明 | Process for abstracting kohlrabi pigment |
| CN102048662A (en) * | 2009-10-30 | 2011-05-11 | 爱普香料集团股份有限公司 | Method for manufacturing rose water containing natural rose pigment |
| CN103044953A (en) * | 2011-10-13 | 2013-04-17 | 黎嘉纯 | Extraction process for Chinese rose pigment |
| CN102838884A (en) * | 2012-01-18 | 2012-12-26 | 天津天康源生物技术有限公司 | Prevention method of anthocyanin pigment crystal precipitate |
| CN105106047A (en) * | 2015-08-07 | 2015-12-02 | 刘学勤 | Water-replenishing and skin-tendering mask |
| CN105693678A (en) * | 2016-01-15 | 2016-06-22 | 甘肃寿鹿山药业有限公司 | Method for extracting procyanidine from grape seeds |
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| CN106726868A (en) | 2017-05-31 |
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