KR20150092564A - Cosmetic composition comprising the extract of plant - Google Patents

Abstract

The present invention relates to a cosmetic composition including a plant extract which contains a plant extract from Chrysanthemum indicum, Macrophylla, and Broussonetia kazinoki var. humilis as an active ingredient and, preferably is characterized by mixing the plant extracts from the Chrysanthemum indicum, Macrophylla, and Broussonetia kazinoki var. humilis by 1:1:1, thereby being used as a cosmetic composition for preventing skin aging and whitening by controlling activity of tyrosinase.

Description

COSMETIC COMPOSITION COMPRISING THE EXTRACT OF PLANT [0002]

The present invention relates to a cosmetic composition containing a plant extract, and more particularly to a composition comprising a plant extract derived from Aji mackerel, a bear, and a plant extract of a bear to form a tyrosinase activity which plays a key role in the melanin biosynthesis process, To a cosmetic composition containing a plant extract having an anti-aging effect and a whitening effect by effectively inhibiting the production of melanin pigment.

The skin color of a person largely depends on the amount of melanin pigment contained mainly in skin cells. People with a lot of melanin pigments have brown or black skin, while those with very low melanin pigment cause vitiligo. Melanin pigment is produced from tyrosine by tyrosinase in pigmented cell called melanocyte which exists in basal membrane of skin. Melanin pigment protects skin from excessive ultraviolet rays, and it plays an important role in suppressing skin damage and skin cancer caused by light rays. However, when melanin is excessively produced by daylight, hormone changes, inflammation, medicines, etc., the pigment of the skin is deposited, which causes cosmetic problems. For example, skin pigmentation caused by hyperpigmentation of the skin is due to an increase in the amount of melanin pigment, which means that the production of melanin pigment is promoted or the number of melanocytes is increased.

Accordingly, in recent years, various whitening cosmetics and medicines for preventing and improving such problems have been developed and marketed. Currently, in the development of a whitening agent for skin cosmetics, a method of decolorizing the resulting melanin pigment and a method of inhibiting the activity of tyrosinase, an enzyme that forms a melanin pigment, are known. However, it is known that the whitening agent using tocopherol or the like, which is used for reducing the melanin pigment, has little discoloration effect on the melanin pigment. Accordingly, inhibitors that inhibit the activity of tyrosinase and inhibit the production of melanin pigment have been attracting attention.

Tyrosinase is an enzyme that catalyzes the oxidation of monophenols and is contained in the melanocytes of higher animals. Tyrosine is oxidized by this enzyme and melanin pigment is produced through a series of chemical reactions and oxidation. This melanin pigment is brown or black, which absorbs excess light in vivo, which adversely affects skin beauty. In view of the fact that the activity of tyrosinase is inhibited, the production of melanin pigment can be inhibited. In the field of conventional cosmetics, natural plant extracts and the like are used as inhibitors of tyrosinase activity as natural whitening extracts.

Korean Patent Publication No. 10-2006-0121496 (published on November 29, 2006)

DISCLOSURE Technical Problem Accordingly, the present invention has been made in order to solve the problems of the prior art described above, and it is an object of the present invention to provide a composition for preventing skin aging and skin whitening by inhibiting tyrosinase activity and inhibiting melanin production, And a cosmetic composition for preventing skin aging and whitening.

In order to achieve the above object, the cosmetic composition containing the plant extract of the present invention is characterized by containing a plant extract from a mung bean, a bear, and a bear mackerel as an active ingredient.

Preferably, the plant extracts from the mung bean, the bear's head,

1: 1: 1, respectively.

Preferably, the plant extract is contained in an amount of 0.0001 to 30.0% based on the total weight of the cosmetic composition.

As described above, according to the skin anti-aging and whitening cosmetic composition containing the plant extract according to the present invention, the activity of tyrosinase is inhibited by mixing the plant extracts from the baby tree, And can be usefully used as a cosmetic composition for prevention and whitening.

1 is a graph showing the effect of a cosmetic composition containing a plant extract on the inhibition rate (%) of tyrosinase activity according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the skin aging prevention and whitening cosmetic composition containing the plant extract of the present invention will be described in detail with reference to the accompanying drawings.

The cosmetic composition according to one embodiment of the present invention comprises a mixture of an extract of Aji Mudberry, a bean curd extract, and a ginger extract. Preferably, the skin anti-aging and whitening cosmetic composition according to an embodiment of the present invention is prepared by mixing Aji mudberry extract, Bear's chrysanthemum extract, and Chrysanthemum root extract at a ratio of 1: 1: 1.

Brousonetia kazinokivar. Humilis is a plant belonging to the genus Phalaenopsis, and includes roots, stems, leaves or fruit including its natural, hybrid or variant. It is a deciduous broad-leaved shrub that is native to Korea and grows in Wibong, Nasan, Ulleungdo and Wando. It grows about 1m in length and its leaves are alternate phyllotaxis. It is egg-shaped or oval-shaped. Its tip is long-pointed, its lower part is round or heart-shaped, its edge is serrated, its front is rough and its length is 5 ~ 8cm. The flowers bloom in May and June as one male and one female. The male flower inflorescence is running on the lower part of the young branch, 1.5cm in length and elliptical, and the female flower petal is rounded from the axil of the leaf. Male flowers have 4 stamens and female flowers have 2 to 4 ends. Fruits ripen in June ~ July, round, diameter 6-7mm.

Corus macrophylla is a plant belonging to the Tertiary family, which includes roots, stem, leaves or fruit including its natural, hybrid or variant. The bear's head is a deciduous broad-leaved arboreous tree distributed in Korea, China, Japan, and the Himalayas. It grows in the forests of the southern part of Korea. It grows well on beaches and downtown areas regardless of shade and sunshine. It is 15 m high and its bark is grayish brown. It is irregularly cracked vertically. Leaves opposite to each other are wide elliptical with plain edge and grayish white on the back. There are many hairs on both sides, especially on the back side, and there are more than 6 ~ 10 pairs of side veins. Yellow flowers are yellowish-white flowers from July to August at the end of branches at the end. Inflorescence has no hairs and has lanceolate petals. Nuclear fruits ripen in black in October.

Chrysanthemumindicum is a plant belonging to the family Asteraceae, which includes roots, stems, leaves or fruit including its natural, hybrid or variant. It is a perennial herb that grows in mountains and fields throughout the country. Growing environment grows in sunny or semi-shrubs, 30-80cm tall, leaves 3-5cm long, 2.5-4cm wide, cracked deep like a bird's wings and serrated at the end. The flower is yellow and hangs as it spreads on the ends of the stem and branch. The diameter is about 2.5cm. The fruit grows around December and contains a lot of small seeds.

Accordingly, the inventors of the present invention have studied the native plant extracts of Jeju Island in order to find a plant natural substance having an effect on skin whitening. As a result, it has been found that the extract of the present invention, The present invention has been completed.

As used herein, the term "extract" refers to a substance having activity of inhibiting tyrosinase activity and inhibiting melanin production, which are isolated from natural products. In addition, the extract in the present invention is used to include not only the extract but also its dry powder or any form formulated using the same.

In the present invention, the plant extract may be extracted using water, an organic solvent, or a mixed solvent thereof, preferably, an organic solvent. The extracted liquid can be used in liquid form or can be used by concentration and / or drying.

In the case of extraction using an organic solvent, an organic solvent such as methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF) (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof. The extract can be extracted at room temperature or warmed under the condition that the active ingredient of the extract is not destroyed or minimized. Examples of the extraction method include, but are not limited to, cold extraction, ultrasonic extraction, and reflux cooling extraction.

Filtration is a process of removing suspended solid particles from an extract, and may be performed by filtering particles using a cotton, nylon, or the like, or by ultrafiltration, freezing filtration, centrifugation, etc., but is not limited thereto. In addition, it may further comprise a separation process by various chromatographies (size, charge, hydrophobicity or affinity chromatographic). The step of drying the filtrate includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying and the like. In some cases, a step of pulverizing the final dried extract may be added.

In one embodiment of the present invention, the mixed extracts from the plants of the present invention include two or three times the weight (kg) of dried rice bran, To 20 times, preferably 3 times to 15 times water; C1 to C4 lower alcohols or a mixed solvent thereof, preferably an ethanol or methanol solvent; 10 to 40 DEG C, preferably 15 to 37 DEG C extraction temperature; 5 hours to 15 days, preferably 3 days to 14 days; 1 to 5 times, preferably 1 to 3 times; The obtained extract was collected by filtration and concentrated under reduced pressure, using a cold filter, ultrasonic extraction, reflux cooling extraction, preferably cold extraction method. The mixture of the Aji mackerel extract, the bear extract and the green tea extract obtained by the above method effectively inhibits tyrosinase activity and melanin production, thereby preventing aging of the skin and exerting skin whitening effect. In addition, since the plant extract has little toxicity and side effects, it can be safely used in the cosmetic composition of the present invention. The combined extracts of Aji mackerel extract, bear extract, and horsetail extract of the present invention obtained by the above production method are added to the composition in an amount of 0.0001 to 5% by weight, preferably 0.001 to 5% by weight, more preferably 0.001 To 3% by weight.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are intended to further illustrate the present invention and are not intended to limit the scope thereof.

Example  1: Preparation of water-soluble extract according to the present invention

1 kg of the completely dried rice paddy, bear bear and red bean curd mixed sample was added to 3 l of distilled water and extracted at 15 to 37 ° C for 14 days in an extractor equipped with a condenser. The extract was filtered with a 400-mesh filter cloth, aged at 5 to 15 ° C for 7 days, and then filtered through filter paper No. 2 of Wattsman. These plant extracts were concentrated under reduced pressure in a distillation apparatus equipped with a cooling condenser to obtain 29 g, 30 g and 29 g (dry weight), respectively.

Example  2: Preparation of ethanol-soluble extract according to the present invention

One kilogram of the completely dried rice husk, bear's bean curd and bean curd mixed sample was added to 3 liters of ethanol and extracted at 15 to 37 ° C for 14 days in an extractor equipped with a condenser. The extract was filtered with a 400-mesh filter cloth, aged at 5 to 15 ° C for 7 days, and then filtered through filter paper No. 2 of Wattsman. These plant extracts were concentrated under reduced pressure in a distillation apparatus equipped with a cooling condenser to obtain 31 g, 39 g and 37 g (dry weight), respectively.

Example  3: Preparation of methanol-soluble extract according to the present invention

One kilogram of the completely dried rice paddy, bear bear and red bean curd mixed sample was added to 3 liters of methanol and extracted at 15 to 37 ° C for 14 days in an extractor equipped with a condenser. The extract was filtered with a 400-mesh filter cloth, aged at 5 to 15 ° C for 7 days, and then filtered through filter paper No. 2 of Wattsman. These plant extracts were concentrated under reduced pressure in a distillation apparatus equipped with a cooling condenser to obtain 38 g, 41 g and 39 g (dry weight), respectively.

Experimental Example  : The inhibitory effect of tyrosinase in a composition containing a plant extract according to the present invention

The inhibitory effect of tyrosinase was measured on the mixed rice bran, bears' mackerel, and red bean paste obtained in Example 2 above. Tyrosinase is an enzyme purified from potato or mushroom. In this experiment, Sigma (Sigma) extracted from mushrooms was used. First, tyrosine, a substrate, was dissolved in a sodium phosphate buffer solution (0.1M, pH 6.8) to prepare a solution having a concentration of 0.3 mg / ml. 334 占 퐇 of the solution was put into a test tube, Solution (0.1 M, pH 6.8). The dried plant extract of Example 2 was dissolved in methanol to a concentration of 10 mg / ml and then diluted to 5, 1.5, 1 mg / ml, 500, 150 and 100 占 퐂 / ml using methanol. Each of the diluted extract samples was taken in an amount of 70 μl each, added to the solution in the test tube, and reacted in a 37 ° C thermostat for 10 minutes.

1M Phosphate Buffer (PB) Sterilize pH 6.8 at 121 ° C for 20 minutes (room temperature: 25 ° C). Make 1M Phosphate Buffer (PB) pH 6.8 to 1/15 M (final concentration = 66.6 mM). Dissolve L-DOPA in 2.0 mM phosphate buffer (pH 6.8). It is dissolved in phosphate buffer (pH 6.8) with Tyrosinase (46 units / ml) and dispensed in 1.5 ml tube. Blank (2 wells) is treated with 2 μl of DMSO and 120 μl of PB in a 96-well plate. Control (2 wells) is treated with 2 μl of DMSO, 120 μl of PB and 40 μl of tyrosinase. In the experimental group, 2ul of sample, 120ul of PB and 40ul of tyrosinase are treated. Add 40 μl of L-DOPA solution (2 mM) to each well. The reaction is carried out at 37 ° C for 10 minutes, and the OD value is measured at 475 nm.

In order to measure the inhibitory effect of tyrosinase on arbutin, arbutin was dissolved in DMSO to a concentration of 10 mg / ml and further diluted with DMSO at 5, 1.5, 1 mg / ml, 500, 150, / Ml to give an experimental group.

The activity inhibition rate was calculated using the following equation (1). The experimental results are shown in Table 1.

As shown in the above Table 1, the mixture of Aji mackerel, bears' bean curd and bean curd extract had a 30 times higher ability to inhibit the activity of tyrosinase than arbutin.

1 is a graph showing the effect of a cosmetic composition containing a plant extract on the inhibition rate (%) of tyrosinase activity according to an embodiment of the present invention. As shown in Fig. 1, it was found that the extracts of Bombyx mori extract, Bombyx mori extract and Bombyx mori extract were superior to those of Bombyx mori extract and a mixture of Bombyx mori extract, a mixture of Bombyx mori extract and Bombyx mori extract, It can be confirmed that the inhibition rate of tyrosinase activity is high in the case of the mixture of the extracts. Accordingly, the plant extract mixture of Ganoderma lucidum, Bear sprout, and Aji Mackay tree according to the present invention effectively inhibits the production of melanin pigment due to tyrosinase activity, which is essential for the melanin biosynthesis process, thereby preventing skin aging and whitening .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the invention.

Claims (4)

A cosmetic composition comprising, as an active ingredient, a botanical extract from Bombyx mori, a bear's head of a bear, and a sweet potato tree.
The method according to claim 1,
The plant extract,
A cosmetic composition comprising a plant extract as an active ingredient, which is extracted with a solvent selected from water, an organic solvent or a mixed solvent thereof.
The method according to claim 2,
The organic solvent may include,
Wherein the cosmetic composition is at least one selected from the group consisting of ethanol, methanol, butanol, acetone, ethyl acetate, hexane, propanol, butyl run glycol and propylene glycol.
The method according to claim 1,
The plant extract,
A cosmetic composition comprising a plant extract as an active ingredient, wherein the cosmetic composition comprises 0.0001 to 30.0% of the total weight of the cosmetic composition.

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