KR20150092606A - Cosmetic composition comprising the extract of plant - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a plant extract, which contains plant extracts from Yellow-filamentous meadow-rue, Maackia fauriei, and wormwood as active ingredients, thereby being useful as a cosmetic composition for preventing skin aging and whitening by inhibiting activity of tyrosinase.

Description

COSMETIC COMPOSITION COMPRISING THE EXTRACT OF PLANT [0002]

The present invention relates to a cosmetic composition containing a plant extract, and more particularly, to a tyrosinase activity which plays a key role in the melanin biosynthesis process by containing plant extracts from legs of golden pheasant, To a cosmetic composition containing a plant extract having an anti-aging effect and a whitening effect by effectively inhibiting the production of melanin pigment.

The skin color of a person largely depends on the amount of melanin pigment contained mainly in skin cells. People with a lot of melanin pigments have brown or black skin, while those with very low melanin pigment cause vitiligo. Melanin pigment is produced from tyrosine by tyrosinase in pigmented cell called melanocyte which exists in basal membrane of skin. Melanin pigment protects skin from excessive ultraviolet rays, and it plays an important role in suppressing skin damage and skin cancer caused by light rays. However, when melanin is excessively produced by daylight, hormone changes, inflammation, medicines, etc., the pigment of the skin is deposited, which causes cosmetic problems. For example, skin pigmentation caused by hyperpigmentation of the skin is due to an increase in the amount of melanin pigment, which means that the production of melanin pigment is promoted or the number of melanocytes is increased.

Accordingly, in recent years, various whitening cosmetics and medicines for preventing and improving such problems have been developed and marketed. Accordingly, there is known a method of decolorizing melanin pigment generated by the development of a whitening agent for skin beauty and a method of inhibiting the activity of tyrosinase, an enzyme that forms a melanin pigment. However, it is known that the whitening agent using tocopherol, vitamins and the like, which is used for reducing the melanin pigment, has little discoloration effect of the melanin pigment. Accordingly, inhibitors that inhibit the activity of tyrosinase and inhibit the production of melanin pigment have been attracting attention.

Korean Patent Publication No. 10-2011-0121775 (published on November 11, 2011).

DISCLOSURE OF THE INVENTION It is therefore an object of the present invention to provide a composition for preventing skin aging and skin whitening by inhibiting tyrosinase activity and inhibiting melanin production, And a cosmetic composition for preventing skin aging and whitening.

In order to achieve the above object, the cosmetic composition containing the plant extract of the present invention is characterized by containing plant extracts from legs of golden pheasant, sorghum, and persimmons as active ingredients.

Preferably, the plant extract is contained in an amount of 0.0001 to 5% by weight based on the total weight of the cosmetic composition.

As described above, according to the skin anti-aging and whitening cosmetic composition containing the plant extract according to the present invention, the activity of tyrosinase is inhibited by mixing plant extracts from legs of golden pheasant, sorghum, And can be usefully used as a cosmetic composition for prevention and whitening.

1 is a graph showing the effect of a cosmetic composition containing a plant extract on the inhibition rate (%) of tyrosinase activity according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the skin aging prevention and whitening cosmetic composition containing the plant extract of the present invention will be described in detail with reference to the accompanying drawings.

The cosmetic composition according to one embodiment of the present invention comprises a mixture of a legume extract, a sorbee extract and a manure extract.

Thalictrum rochebrunianum, which is used as an active ingredient in the cosmetic composition of the present invention, is a plant belonging to the Mineral reptile family distributed in Korea (Gangwon province, Kyonggi province, Pyongan province) and Japan, and the flowering time is bloomed from June to August, It grows. Height is 70 ~ 100cm, there is no hair in the whole, stem is straight, branch is usually purple. Leaves are alternate phyllotaxis with short petiole, three to four times with three small leaves, and the stipule is flat. The small leaf is an inverted egg shape or a wide inverted egg shape. It is 2 ~ 3cm long and 1.5 ~ 2.5cm long with 3 sawtooths at the end. The stipule is egg-shaped (membrane quality: translucent like thin paper) and encloses the stem, and the back side is white (powder white). In July ~ August, a purplish flower blooms on the end of the stem or on the axillae of the cone. There are no petals, 4 calyxes are oval. There are many pistils and stamens, the operation is about 8mm in length, and the anther is yellow with the operating table. Fruits are 8 ~ 20 spindle, winged ridgeline, and long elliptical. The plant of the same genus is larger than the legs of the pheasant, and the whole is large and the leaves are divided into 2 ~ 3 pieces. It is a medicinal herb that has the effect of lowering fever, removing warts and detoxifying rootstocks and roots. As used herein, the term "golden pheasant leg" refers to various organs (e.g., leaves, flowers, fruits, stems, branches, roots and outposts) of the legs of a golden pheasant. In the present invention, do.

Maokia fauriei is a deciduous tree belonging to Leguminosae. It is a special plant of Korea and is distributed in a large amount in 1,100 ~ 1,800m range of Jeju Island. It is a Korean species and it is distributed in Halla mountain area, and it grows in the forest. The height reaches 8m. The branches have grayish white hairs and become dark purple or grayish black. Leaves are alternate, once folded and 9 ~ 17 small leaves. The small leaf is oval oval or long oval, 3 ~ 6cm long, 1-2cm wide, with flat edge. Flowers bloom from July to August, with yellowish white spots hanging on the gun inflorescence. The pedicel has hairs, calyx is 3.5mm long, bell-shaped, and divides shallowly into 5 pieces. There are 10 stamens and brown hairs on pistil. The pod is flat oval, 3 ~ 7cm long, 5 ~ 8mm wide, with wings on one side. It is similar to the elder tree, but it has many small leaves and smaller ones. The main components include lectin, (-) mangifan, ononin, wistin, didezin, pomonotenine, 4 ', 7-dihydroxyflavone and genistein, retin, apramycin, dihydroperraine and 3 '-Methoxidase, which is known to have anti-cancer effects. As used herein, the term "sorbee" refers to various organs (e.g., leaves, flowers, fruits, stems, branches, roots and outposts) of the sorbee tree. In the present invention, preferably, do.

Artemisia capillaries Thunberg has been traditionally used as a medicinal herb and is often referred to as a herbal medicine. Mugwort is known as a carnitine, , Loveliness, indulgence, primordiality, affection and so on. The length of the mugwort is about 60 ~ 120cm. The main stem has the vertical line and the whole is covered with the weblike hair. As the rhizome stretches sideways, buds grow out of the place. The leaves that come up from the roots fall down later, and the leaves that come up from the stem have browned leaves. The white hairs are densely on the back of the leaves. The edges are flat or acute and the leaves become smaller as they go up. Flower is bloomed from July to October, 2.5 ~ 3.5mm long, 1.5mm in diameter. There is almost no flower. The leaves contain essential oil, cineol, Tuyon, borneol, paraffin, adenine, choline, vitamins A, B, and C. The leaves of the mugworts contain essential oils, tannins, resins, gummy substances, artemisin, ascorbic acid, carotene, B, D and so on. The roots of mugwort include polysaccharides such as arte moros, essential oil, dihydromatricarindestherol and tetradecadrine, inulin and mucus.

Mugwort is tasty, very warm, and has a warm temperament. It has the efficacy of acid, anthrax, ongye, eugyol, ejaculation, dehumidification, hemostasis, It is used for the treatment of abdominal coldness, diarrhea, muscle spasm, chronic diarrhea and chronic dysentery, hematemesis, non-hemorrhage, hemorrhage, menstrual irregularity, bloating, subarachnoid anxiety,

As used herein, the term " arthropods "refers to various organs (e.g., leaves, flowers, fruits, stems, branches, roots, outposts) of the arthropods.

Galactomyces is a kind of yeast that contains various kinds of useful ingredients such as protein, carbohydrate, lipid, and vitamin. It has a very similar structure to skin cells, and especially helps to improve skin health and various troubles by the metabolites generated after fermentation. It is fast absorbing and has the ability to inhibit skin sedation and melanin formation and inhibit the induction of inflammatory responses. Accordingly, the inventors of the present invention have researched the native plant extracts of the present invention in order to find a plant natural substance effective for skin whitening. As a result, it has been found that the extracts of the plants of the present invention are effective in inhibiting tyrosinase activity and inhibiting melanogenesis Thus completing the present invention.

In the present invention, the plant extract may be extracted using water, an organic solvent, or a mixed solvent thereof, preferably, an organic solvent. The extracted liquid can be used in liquid form or can be used by concentration or drying.

In the case of extraction using an organic solvent, an organic solvent such as methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF) (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof. The extract can be extracted at room temperature or warmed under the condition that the active ingredient of the extract is not destroyed or minimized. Depending on the organic solvent to be extracted, the degree of extraction and the degree of loss of the active ingredient of the extract may differ. Therefore, an appropriate organic solvent should be selected and used. The extraction method is not particularly limited, and examples thereof include cold extraction, ultrasonic extraction, and reflux cooling extraction.

Filtration is a process of removing suspended solid particles from an extract, and may be performed by filtering particles using a cotton, nylon, or the like, or by ultrafiltration, freezing filtration, centrifugation, etc., but is not limited thereto. In addition, it may further comprise a separation process by various chromatographies (size, charge, hydrophobicity or affinity (chromatography)).

The step of drying the filtrate includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying and the like. In some cases, a step of pulverizing the final dried extract may be added.

In one embodiment of the cosmetic composition containing the plant extract according to the present invention, the mixed extract of the legume extract, sorb tree extract and mugwort-powder extract of the present invention of the present invention can be applied to the legs, The extracts were filtered through a filter paper (Wattsman, USA) after repeating 3 times repeatedly at a constant interval by adding 1 L of alcohol (100 g powder, 10 times) as a mixture, removing the ethanol at 40 캜 with a vacuum rotary condenser, The obtained extract was obtained by filtration and concentration under reduced pressure.

The extracts of legume, sorbitus extract, or mixture of extracts of Galaxymae obtained by the above method effectively inhibit tyrosinase activity and melanin production, and prevent skin aging and whitening effect of skin I will exert. In addition, since the plant extract has little toxicity and side effects, it can be safely used in the cosmetic composition of the present invention.

The mixed extract of the legume extract, sorbitus extract or Artemisia princeps extract obtained by the above method according to the present invention is added to the composition in an amount of 0.0001 to 5% by weight, preferably 0.001 to 5% by weight, 0.001 to 3% by weight.

Hereinafter, the present invention will be described in more detail by way of examples. The following examples are intended to further illustrate the present invention and are not intended to limit the scope thereof.

Example  1: Method for producing ethanol-soluble extract according to the present invention

100 g of the powder obtained by thoroughly drying the dried mixture of the golden pheasant legs, the sorb tree and the aruguswort were sieved, washed with ice, and extracted with 3 times repeatedly at intervals. The paddy (Wattmann, USA) The extract was filtered through a vacuum rotary evaporator to remove ethanol at 40 ° C and then concentrated.

Experimental Example  1: Tyrosinase inhibitory effect of the composition containing the plant extract according to the present invention

The extracts of the golden pheasants such as the pheasant's legs, the sorb tree and the mugwort, the extracts of the golden pheasant's legs and the sourbee mixed extract, the mixed extracts of the golden pheasant's legs and mugwort, the mixed extracts of the mulberry leaves and the mugwort, And tyrosinase inhibitory effect on arbutin were measured. Tyrosinase is an enzyme purified from potato or mushroom. In this experiment, tyrosinase extracted from mushrooms was used. First, phosphate buffer (PB) pH 6.8 is sterilized at 121 ° C for 20 minutes and cooled (room temperature: 25 ° C). Dissolve L-DOPA in 2.0 mM phosphate buffer (pH 6.8). After dissolving in phosphate buffer (pH 6.8) with Tyrosinase (46 units / ml), dissolve in 1.5 ml tube and dissolve in frozen storage. Prepare 1M Phosphate Buffer (PB) pH 6.8 in a 96 well plate to 1/15 M (final concentration = 66.6 mM) before the experiment. Blank, Control (2wells), 2ul of sample, PB 120ul; Treat 40 Ty of tyrosinase. Add 40 μl of L-DOPA solution (2 mM) to each well. The reaction is carried out at 37 ° C for 10 minutes, and the OD value is measured at 475 nm. The activity inhibition rate is calculated by the following equation (1).

Blank (2 wells): 100 μl DMSO 2 μl, PB 160 μl, not treated

Control (2 wells): 2 μl of 100% DMSO, 120 μl of PB, 40 μl of Tyrosinase

Plant Extract Sample: 2 μl of 100% DMSO, 120 μl of PB, 40 μl of tyrosinase

Blank, Control, and 40 ㎕ of L-DOPA solution (2mM) in plant extract samples.

After reacting for 10 minutes in a 37 ° C thermostat, the absorbance at 475 nm was measured with an analytical system.

The blanks of the experimental group were reactants except for the tyrosinase solution, i.e., the reactants consisting of tyrosine, seaweed and plant extract, and sodium phosphate buffer solution. The blank of the control group was obtained by adding methanol instead of the plant extract in the blank of the experimental group Respectively. When measuring the inhibitory effect of tyrosinase on arbutin, arbutin was dissolved in a sodium phosphate solution to a concentration of 10 mg / ml, and then 5 times dilution was repeated 4 times with sodium phosphate buffer solution. Respectively. The blank of the experimental group of arbutin is a reactant consisting of a reagent except tyrosinase, that is, a tyrosine, an arbutin solution, and a sodium phosphate buffer solution. The control group of arbutin was a sodium phosphate buffer solution instead of the arbutin solution, and the blank of the arbutin control group was a solution containing the tyrosinase solution, i.e., tyrosine, sodium phosphate buffer solution. The above contents are summarized in Table 1 below.

The inhibition rate of tyrosinase for plant extracts and control was calculated using the following equation (2). The experimental results are shown in Fig.

A: absorbance of the control group B: blank absorbance of the control group

C: absorbance of experimental group D: blank absorbance of experimental group

1 is a graph showing the effect of a cosmetic composition containing a plant extract on the inhibition rate (%) of tyrosinase activity according to an embodiment of the present invention. As shown in FIG. 1, the extract of Artemisia juncea, the legs of Golden Pheasant, and the mixture of Solbien showed high whitening efficacy in inhibiting the activity of tyrosinase as compared to the single extract or other mixed extracts.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the invention.

Claims (4)

A cosmetic composition comprising a golden pheasant legs extract, a sorb tree extract, a starch extract and a galactomith.
The method according to claim 1,
The plant extract,
A cosmetic composition comprising a plant extract as an active ingredient, which is extracted with a solvent selected from water, an organic solvent or a mixed solvent thereof.
The method according to claim 2,
The organic solvent may include,
Wherein the cosmetic composition is at least one selected from the group consisting of ethanol, methanol, butanol, acetone, ethyl acetate, hexane, propanol, butyl run glycol and propylene glycol.
The method according to claim 1,
The plant extract,
A cosmetic composition comprising a plant extract as an active ingredient, wherein the cosmetic composition contains 0.0001 to 5% by weight based on the total weight of the cosmetic composition.

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