KR20180100787A - Skin-lightening Composition Using an Extract of Shindari - Google Patents

Abstract

The present invention discloses compositions for skin whitening using complex extracts of petals of Shindari and Rhododendron weyrichii Maxim, showing melanin production inhibitory activity in a concentration-dependent manner when treated with B16F10 cells, a mouse melanoma cell line stimulated with α-MSH, which is a melanin production inducer. An object of the present invention is to provide the compositions for skin whitening using complex extracts of petals of Shindari and Rhododendron weyrichii Maxim. The present invention provides the compositions for skin whitening of the present invention is characterized by comprising the complex extracts of petals of Shindari and Rhododendron weyrichii Maxim as an active ingredient.

Description

[0001] The present invention relates to a skin-lightening composition using an extract of a plant extract,

The present invention relates to a composition for whitening skin using a mixed extract of Schizophyllum sp.

Skin is the largest organ that protects the inside of our body from various external environments, and at the same time is the outmost body of our body. Such skin contains keratinocytes that inhibit the entry of foreign substances, Langerhans cells or macrophages that are responsible for skin immunity, fibroblasts that maintain skin tissue, and pigmentation. (Melanocytes) are present.

Melanin is a black pigment that is synthesized in melanocytes. It is a protein complex that protects skin cells from the external environment such as ultraviolet rays. However, the overproduction of melanin can form spots, freckles, etc. in the human body and can even cause skin cancer (JP Ortonne and JJ Nordlund, JJ Norlund, RE Boissy, VJ Hearing, RA King and JP Ortonne, 489, Oxford University Press, New York, 1998).

This melanin is produced by the action of tyrosinase and tyrosinase related proteins present in melanocytes. Specifically, tyrosine is oxidized by tyrosinase to DOPA (3,4-dihydroxyphenylalanine), and then dopa is oxidized again by the action of dopaoxidase to form dopaquinone And is produced by the action of tyrosinase related protein TRP-1 (5,6-dihydroxy indole-2-carboxylic acid oxidase) and TRP-2 (dopachrome tautomerase). It is known that tyrosinase, TRP-1 and TRP-2 are promoted by a transcription factor MITF (microphthalmia-associated transcription factor) and MITF is inhibited when ERK (extracellular signal-regulated kinase) Kim, et al., J Cell Sci. 2003; 116: 1699-706).

Since tyrosinase is involved as a first step in the production of melanin, studies on whitening agents have focused on the search for tyrosinase inhibitors (Kor. J. Pharmacogn., 44 (3): 220, 2013). Most of the whitening agents currently in use are these tyrosinase inhibitors, and hydroquinone or its glycosides arbutin (β-D-glucopyranoside) and kojic acid all have tyrosinase inhibitory activity These are whitening agents. Hydroquinone or its glycoside arbutin (β-D-glucopyranoside) competitively inhibits tyrosinase activity and kojic acid chelates the tyrosinase active site, resulting in tyrosine (Korean J. Plant Res., 26 (5): 526, 2013; J. Korean Soc. Food Sci. Nutr., 41 (2): 156, 2012) .

However, these whitening agents are synthetic whitening agents. These synthetic whitening agents have adverse effects such as dermatitis, erythema, vitiligo, and burn in long-term use, and in some countries such as Taiwan, allowable concentrations are set without prescription by medical experts. Due to the safety problem of the synthetic whitening agent, researches for finding a whitening agent in a natural product, which has relatively little irritation or side effects on the skin of the human body, have been actively conducted.

On the other hand, the hoof leg is a low-concentration alcoholic beverage made by fermenting rice, boiled rice, or rice that has begun to rest a little. It is also referred to as a primordial or tangible. It is derived from using it when it is difficult to keep the leftover rice. When the rice is fermented and it becomes smutty and can not recognize the shape, it is sieved to sieve raw or boiled. It is fermented for one day or two days in summer and 5 or 6 days in winter. Due to the short fermentation period, the dietary fiber is low to 1%, and sour taste and sweet taste are strong. Thick legs are similar to rice wine with barley (or rice), yeast, and water, but have a short fermentation period and low alcohol content, similar to yogurt than rice wine.

As interest in health has increased recently, there has been a growing interest in using a beneficial fermenting bacteria for the body. It is known that the effect of the back leg on the improvement of the intestinal health and the improvement of the dementia is known, and attempts are being made to apply it to new fields such as cosmetics as well as foods using the useful ingredients. A known study has shown that the composition of rice husk fermented with yeast (Kim SC, 1998, Master thesis, Jeju National University of Korea, pp 3-5) Soc Food Sci Nutr 28 (5): 1017-1021), a study on the characteristics of hoof legs prepared by adding citrus fruits (Kim HS, 2011. Master thesis, Jeju National University of Korea, 40p) (Kim S et al., 2015. Korean J Food Cook Sci. 31 (1) 009 ~ 017), and a method of manufacturing a foot rest with improved presentation (Korean Patent No. 1399625).

The present invention discloses the use of Schizandrae extract as a skin lightening agent based on melanin production inhibitory activity.

Korean Patent No. 1399625

It is an object of the present invention to provide a skin whitening composition using an extract of a mixture of a foot and a Japanese apricot flower petal.

Other and further objects of the present invention will be described below.

The present invention, as confirmed in Examples and Experimental Examples described below, is characterized in that, when succulent plant extract and sesame leaf extract are treated with B16F10 cells, a mouse melanoma cell line stimulated with? -MSH, which is a melanogenesis inducer, melanin Production inhibitory activity, and specifically showed no cytotoxicity. In particular, when the mixture of each extract was administered to a mouse melanoma cell line B16F10 cell stimulated with α-MSH, which is a melanin inducer, melanin production inhibitory activity And that there is a clear tendency to see it.

The present invention provides a skin whitening composition of the present invention, which comprises an extract of Schizandra chinensis and an extract of a Japanese apricot flower, as an active ingredient.

As used herein, the term "extract" refers to a substance which is extracted with water, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, and butanol, methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, - Extracts obtained by leaching using dimethylformamide (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixed solvent thereof, and extracts obtained using supercritical extraction solvents such as carbon dioxide and pentane Extract means the fraction obtained by fractionating the extract or the extract. The extraction method can be applied by any method such as cold rolling, refluxing, heating, ultrasonic irradiation, supercritical extraction, etc. in consideration of the polarity, extraction degree, . In the case of the fractionated extract, the fraction is suspended in a specific solvent and then mixed with a solvent having a different polarity. The extract is adsorbed on a column filled with silica gel or the like, and then a hydrophobic solvent, a hydrophilic solvent, Quot; means fractions obtained as a mobile phase. Also, the meaning of the extract includes a concentrated liquid extract or a solid extract in which the extraction solvent is removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying and the like. Preferably an extract obtained by using water, ethanol or a mixed solvent thereof as an extraction solvent, and more preferably an extract obtained by using a mixed solvent of water and ethanol as an extraction solvent.

In the present specification, the term "active ingredient" alone means an ingredient which exhibits the desired activity or can exhibit activity together with a carrier which is not itself active.

In the present specification, "skin whitening" can be understood as an increase in skin brightness which is reduced due to excessive production of melanin. Specifically, skin whitening can be improved by improving the skin, freckles, Treatment, prevention, suppression of expression or delay.

The composition for skin whitening of the present invention can be prepared in addition to the active ingredient, including skin whitening ingredients, skin wrinkle improving ingredients, ultraviolet protective ingredients, skin moisturizing ingredients and the like well known in the art in order to reinforce and add skin-related activities . Specific examples of skin whitening ingredients include arbutin, niacinamide, ascorbyl glucoside, alpha-bisabolol and oil soluble licorice (Glycyrrhiza) extract. Examples of the skin wrinkle improving agent include retinol, retinyl palmitate Examples of the ultraviolet protective component include dyes such as drometrizol, drometrizol trisiloxane, dicaloyltriolate, Dimethicyldecibenzalmalonate, diethylhexylbutadamate, Complex extracts such as dithriazone and pine bark extract, phosphatidylserine, finger root extract powder, and complex extracts of red ginseng and mountain juice. Examples of the moisturizing component include AP collagen enzyme digesting peptide, collactive collagen peptide, N-acetyl glucosamine, konjac potato extract, dandelion extract, rice bran extract, corn germ extract, low molecular weight collagen peptide, ground extract powder, phosphatidylserine, hyaluronic acid . For other skin-whitening, wrinkle-reducing, UV-protecting and skin-moisturizing ingredients, please refer to the Functional Cosmetics Promotion Manual ("Standards and Testing Methods for Functional Cosmetics" Quot; Health Functional Food Standards & Specification "). These ingredients may be included in the skin whitening composition of the present invention in combination with one or more thereof.

In the composition for whitening skin of the present invention, the active ingredient may be contained in any amount within a range that does not cause toxicity to the human body, depending on the purpose of use, formulation, etc. Specifically, it is preferably 0.001 to 99.99% % ≪ / RTI > by weight.

The skin whitening composition of the present invention can be identified as a cosmetic composition in a specific embodiment.

Even when the composition of the present invention is identified as a cosmetic composition, the cosmetic composition may be classified into any product category according to the use of the cosmetic composition. Specifically, the cosmetic composition may be a functional cosmetic having a use such as skin whitening, . Specific examples of the product form include a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax Foundation, spray or the like. In a specific product form, it may be a form of flexible lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

The cosmetic composition of the present invention may contain, in addition to its active ingredient, conventional additives such as stabilizers, solubilizing agents, surfactants, vitamins, colorants and antioxidants, and carriers commonly used in cosmetic compositions.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid esters of sorbitan, and the like.

When the formulation of the present invention is a suspension, a carrier, such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention can be produced according to a method for producing a cosmetic composition which is usually carried out in the art, except that it contains an active ingredient showing skin whitening activity.

The skin whitening composition of the present invention can be grasped as a food composition in another specific embodiment.

The food composition of the present invention can be prepared in any form and can be used in various forms such as beverages such as tea, juice, carbonated drink, ionic drink, processed milk such as milk and request route, gum, rice cake, Korean confectionery, Such as foods, tablets, capsules, rings, granules, liquids, powders, flakes, pastes, syrups, gels, jellies, bars and the like. In addition, the food composition of the present invention may be classified into any product category as long as it meets the laws and regulations on the time of manufacture and distribution in the legal and functional category. For example, it may be a health functional food according to the Act on Health Functional Foods, or may be two oils, fermented beverages, special-purpose foods, etc. according to each food type in the Food Safety Act (Food Standards, Food Standards and Standards) of the Food Sanitation Act.

The food composition of the present invention may contain food additives in addition to the active ingredients thereof. Food additives are generally understood to be substances which are added to foods and mixed or infiltrated into food in the manufacture, processing or preservation of food, and their safety must be ensured since they are taken with food daily and for a long time. Food additives according to the Food Hygiene Act (food additives notification, food additive standards and standards) are limited by the classification of safe synthetic food additives as chemical synthetic products, natural additives and mixed preparations.

These functional food additives can be classified into sweeteners, flavors, preservatives, emulsifiers, acidifiers, and thickeners.

Sweetening agents may be used in an amount that sweetens the food in a suitable manner, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavors may be used to enhance taste or flavor, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. Examples of natural flavoring agents include those obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or those obtained from green tea leaves, Asiatica, Daegu, Cinnamon, Chrysanthemum leaves and Jasmine. It may also be obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used.

As the preservative, calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate and EDTA (ethylenediaminetetraacetic acid) can be used. As the emulsifier, acacia gum, carboxymethyl cellulose, Pectin and the like. As the acidulant, math, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid and the like can be used. The acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste.

Examples of the thickening agent include suspending agents, sedimentation agents, gel-forming agents, bulking agents and the like.

The food composition of the present invention may contain physiologically active substances or minerals which are known in the art and which are stable as a food additive in addition to the above-mentioned food additives in order to supplement and supplement functional and nutritional properties.

Examples of such physiologically active substances include catechins contained in green tea and the like, vitamins such as vitamin B1, vitamin C, vitamin E and vitamin B12, tocopherol, dibenzoyl thiamine, etc. Examples of minerals include calcium preparations such as calcium citrate, magnesium stearate , Iron preparations such as iron citrate, chromium chloride, potassium iodide, selenium, germanium, vanadium, zinc and the like.

The food composition of the present invention may contain an appropriate amount of the above-mentioned food additives according to the product type so as to achieve the purpose of addition thereof.

With regard to other food additives that can be included in the food composition of the present invention, reference may be made to the Food Code or the Food Additive Code.

The skin whitening composition of the present invention can be identified as a pharmaceutical composition in a specific embodiment.

When the composition for skin whitening of the present invention is identified as a pharmaceutical composition, the target disease can be understood as skin hypercholesterolemia. Specific examples of such hypercholesterolaemia include freckles, senile spots, liver, spots, brown or black spots, Hyperglycemia after drug use such as cyanic melasma, calcium antagonist, sequela due to sclerotherapy, gravidic chloasma, mellitus in women taking oral contraceptives, But are not limited to, post-inflammatory hyperpigmentation due to scratches or dermatitis, including scratches and burns, phototoxic response or other similar small fixed pigmented lesions.

The pharmaceutical composition of the present invention may be prepared into oral formulations or parenteral formulations according to the route of administration by conventional methods known in the art, including pharmaceutically acceptable carriers in addition to the active ingredient. The term "pharmaceutically acceptable" as used herein means that the application (prescribing) subject does not have the above-mentioned toxicity that is adaptable without inhibiting the activity of the active ingredient.

When the pharmaceutical composition of the present invention is prepared into an oral formulation, it may be formulated into powder, granules, tablets, pills, sugar tablets, capsules, solutions, gels, syrups, suspensions, wafers And the like. Examples of suitable pharmaceutically acceptable carriers include sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol, xylitol, starch such as corn starch, potato starch and wheat starch, cellulose, methylcellulose, ethylcellulose, Cellulose derivatives such as sodium carboxymethyl cellulose and hydroxypropylmethyl cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, And the like. In case of formulation, a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, and / or an excipient may be formulated according to need.

When the pharmaceutical composition of the present invention is prepared into a parenteral dosage form, it may be formulated in the form of an injection, transdermal drug delivery, nasal aspirate and suppository together with a suitable carrier according to methods known in the art. Examples of suitable carrier in the case of injection preparation include sterilized water, polyol such as ethanol, glycerol and propylene glycol, or a mixture thereof. Preferably, the carrier is selected from the group consisting of Ringer's solution, PBS (phosphate buffered saline) containing triethanolamine, , 5% dextrose, and the like may be used. When formulated with a transdermal drug, it can be formulated in the form of an ointment, a cream, a lotion, a gel, a solution for external use, a pasta, a liniment, or an air-roll. The nasal inhalant may be formulated in the form of an aerosol spray using a suitable propellant such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, etc. The suppositories may be witepsol, polyethylene glycol, cacao butter, laurin, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearates, sorbitan fatty acid esters and the like can be used.

The formulation of pharmaceutical compositions is well known in the art and can be specifically described in Remington ' s Pharmaceutical Sciences (19th ed., 1995). This document is considered part of this specification.

The preferred dosage of the pharmaceutical composition of the present invention is 0.001 mg / kg to 10 g / kg per day, preferably 0.001 mg / kg to 1 g / day, depending on the patient's condition, body weight, sex, age, / kg < / RTI > The administration can be carried out once or several times a day. Such dosages should in no way be construed as limiting the scope of the invention.

As described above, according to the present invention, it is possible to provide a composition for skin whitening using a mixed extract of Schizophyllum sp.

The skin whitening composition of the present invention can be manufactured into foods, cosmetics, medicines and the like, and the side effects can be relatively small in comparison with the synthetic skin whitening agents, since the whitening agents have been used for edible purposes.

Fig. 1 shows cytotoxicity results of B16F10 cells, which are mouse melanoma cell lines such as Shinku's extract.
FIG. 2 shows the results of showing that the herbal extract has inhibitory activity on melanin formation in B16F10 cells, a mouse melanoma cell line stimulated with? -MSH, which is a melanin-forming stimulant.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

<Examples> Production of flower petal extract and succulent plant extract

<Example 1> Preparation of an extract of a Japanese persimmon tree

Chamkkot Tree (Rhododendron weyrichii) then was added to 70% ethanol of 10 times the weight of the petal powder extracted at room temperature for 24 hours and filtered with a filter paper. The filtrate was concentrated under reduced pressure and lyophilized to obtain an extract of Spodoptera litura.

Example 2 Preparation of Schizophyllum Extract

70% ethanol in a weight of 10 times was added to the hoofed powder and extracted at room temperature for 24 hours, followed by filtering with a filter paper. The filtrate was concentrated under reduced pressure and lyophilized to obtain an extract of Spodoptera litura.

<Experimental Example> Confirm whitening activity

<1> Experimental Method

<1-1> Cell culture

B16F10 cells, a melanoma cell line, were purchased from the Korean Cell Line Bank and were cultured in RPMI 1640 supplemented with 1% antibiotic (Gibco, USA) and 10% fetal bovine serum (FBS; Gibco, Grand Island, USA) (Dulbecco ' s modified Eagle ' s medium) at 37 &lt; 0 &gt; C in a 5% CO ₂ incubator and subcultured once every 4 days.

<1-2> Cytotoxicity Assessment

MTT analysis was performed to determine the effect of the sample of the example on cell growth. In living cells in which the metabolism is vigorous, MTT (3- (4,5-dimethylthiaxo-2-yl) -2,5-diphenyl tetrazolium bromide (Sigma, MO, USA ) To form a purple, water-insoluble formazan.

B16F10 cells were cultured in a 96-well plate at a density of 2.0 × 10 ⁴ cells / mL using DMEM supplemented with 10% FBS and cultured for 18 hours. Then, 50 μl of the MTT solution was added and reacted for 4 hours. After the culture medium was completely removed and 200 μl of dimethylsulfoxide was added to completely dissolve the precipitate, the absorbance at 540 nm was measured using a microplate reader. The average absorbance value of each sample group was determined and compared with the absorbance value of the control group, the cell growth rate was evaluated.

<1-3> Measurement of Melanin Biosynthesis Inhibitory Activity

B16F10 cells cultured in DMEM medium were seeded on a 24-well plate at a density of 2.0 × 10 ⁴ cells / well and cultured for 24 hours. Simultaneous treatment with 100 nM α-MSH (-melanocyte stimulating hormone) Time. The cells were then washed with phosphate buffer (pH 7.4), and lysed in a cell lysis buffer (100 mM sodium phosphate (pH 6.8), 0.1 mM PMSF, 1% Triton X-100) at -80 ° C for 30 minutes. The following cell lysate was added with 1N NaOH and reacted at 80 ° C for 1 hour to dissolve the precipitate. The absorbance was measured at 405 nm using a spectrophotometer. Inhibition of melanin biosynthesis was expressed by the absorbance reduction ratio of the sample solution and the non-added sample.

&Lt; 2 & Experiment result

<2-1> Cytotoxicity measurement results

B16F10 cells were treated with the extract of Example and cultured for 48 hours. The cell viability was confirmed using MTT assay [Fig. 1]. Referring to FIG. 1, it can be confirmed that all of the extracts of the Examples do not show cytotoxicity.

<2-1> Melanin synthesis amount measurement result

To evaluate the possibility of using the extract of Example of the present invention as a natural whitening agent, B16F10 cells, a mouse melanoma cell, were co-treated with the extract of Example and melanin inducer, alpha-MSH, to determine whether melanin production induced by? , And the results are shown in Fig. Referring to FIG. 2, it can be seen that the production of melanin induced by? -MSH is decreased in a concentration-dependent manner by the extract of the Example.

Particularly, it can be seen that the effect of the mixture of the extract of the Japanese apricot kernel oil and the extract of the succulent plant (1: 1 by weight ratio) in the extract of the example of the present invention is remarkably reduced.

Claims (5)

A composition for skin whitening comprising an extract of a mixture of a plant extract and a saccharide tree petal as an active ingredient.
The method according to claim 1,
Wherein the extract is obtained by water, ethanol, or a mixed solvent thereof.
3. The method according to claim 1 or 2,
Wherein the composition is a cosmetic composition.
3. The method according to claim 1 or 2,
Wherein the composition is a food composition.
3. The method according to claim 1 or 2,
Wherein the composition is a pharmaceutical composition.

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