KR102265404B1 - Cosmetic composition for comprising benzyl glycol extract of Hibiscus and Pawwpaw - Google Patents

Abstract

The present invention relates to a cosmetic composition for moisturizing the skin, maintaining and improving skin elasticity, and preventing skin wrinkling, which includes Geumhwagyu and paw paw paw tree benzyl glycol extract, specifically, benzyl glycol extract of Keumhwagyu beef and paw paw tree leaf as an active ingredient. it's about

Description

Cosmetic composition for comprising benzyl glycol extract of Hibiscus and Pawwpaw

The present invention relates to a cosmetic composition for moisturizing the skin, maintaining and improving skin elasticity, and preventing skin wrinkling, which includes Geumhwagyu and paw paw paw tree benzyl glycol extract, specifically, benzyl glycol extract of Keumhwagyu beef and paw paw paw tree leaf as an active ingredient. it's about

Functional cosmetics are cosmetics with special functions other than cleansing and cosmetic purposes. They are intermediate between cosmetics and pharmaceuticals. They are products with improved efficacy and safety, and are limited to products that protect the skin from wrinkle improvement, whitening, and UV rays.

Among functional cosmetics, the development of anti-aging cosmetics, including wrinkle improvement, is a major area of interest in the cosmetics industry due to the expansion of various consumer groups due to the increase in the aging population, the development of beauty and medical technology, and the growing interest of consumers who want healthy and beautiful skin. became The so-called 'initial wrinkle improvement', which is a so-called 'initial wrinkle improvement' treatment that manages wrinkles from the late 20's or early 30's due to skin aging caused by stress and environmental pollution, etc. The product market is growing.

The most common symptom of skin aging is wrinkles, and the exact mechanism of its occurrence is still unknown, and damage to matrix proteins such as collagen and elastic fibers in the skin is the main cause of wrinkles.

In addition, the skin is one of the tissues that receive the most oxidative stress due to external stimuli as the outermost layer of the body, and various external factors such as ultraviolet rays, drugs, and environmental pollutants increase the generation of active oxygen in the skin and It combines with unsaturated fatty acids in cell membranes to cause oxidative damage, and oxidative stress in the skin is known to be a major factor in wrinkles.

Among the wrinkles that occur due to aging of the skin, small wrinkles are related to the shape of the epidermis and upper dermis of the skin, and large and deep wrinkles and sagging are basically considered to be the cause of dermal aging, that is, qualitative and quantitative changes in collagen and elastin.

As raw materials for wrinkle-improving functional cosmetics, retinol and adenosine, ingredients that have been announced to help improve wrinkle, are used, but these products mainly focus on one specific antioxidant component, which can cause skin troubles as well as remarkable. It has a problem that it is difficult to expect a satisfactory effect. Currently, most bioactive materials used as main raw materials for functional cosmetics depend on imports, so research on new bioactive materials using domestic technology and domestic natural materials is necessary.

1. Korean Patent Publication No. 1020160070892

Therefore, the technical problem to be achieved by the present invention is to provide a cosmetic composition for moisturizing the skin, maintaining and improving skin elasticity, and preventing skin wrinkles using natural materials.

In order to achieve the above technical object, the present invention contains benzyl glycol extract of leaves of Geumhwagyu and benzyl glycol extract of leaves of paw paw paw paw tree as active ingredients, moisturizing the skin, maintaining and improving skin elasticity, and preventing skin wrinkles A cosmetic composition is provided.

The cosmetic composition according to the present invention as described above contains 0.001 to 20% by weight of each of the leaf benzyl glycol extract of Geumhwagyu and the leaf benzyl glycol extract of Paw pawpaw, based on the total weight of the composition.

As described above, the composition according to the present invention may further contain 0.001 to 20% by weight of the benzyl glycol extract from pure vegetable leaves based on the total weight of the composition.

The cosmetic composition according to the present invention has the advantages of remarkably excellent skin moisturizing, skin elasticity maintenance or improvement, skin wrinkle prevention effect, and ultimately skin anti-aging effect.

Hereinafter, specific contents for carrying out the present invention will be described.

In order to achieve the above object, in the present invention, a cosmetic composition for moisturizing the skin, maintaining and improving skin elasticity, and preventing skin wrinkles by containing benzyl glycol extract from leaves of Geumhwagyu and benzyl glycol extract from leaves of paw paw paw paw paw tree as active ingredients. provides

The Geumhwagyu, an annual herbaceous plant belonging to the genus Hibiscus, is also called a wild bouillon, and is a medicinal plant carried in the herbaceous tree. It has been restored and cultivated in China. It is also cultivated in Korea. Geumhwagyu grows taller than a human in July-August, and the diameter of the flower is usually 16-18cm, and the petals are yellow and thin, and the scarlet-colored stamens are very beautiful with dark blue foliage. It is said that it is very important to harvest on time, as the flowering time of golden oxen is only about six hours.

Geumhwagyu flowers are picked from the end of July, cut into small pieces, dried and made into powder. Raw materials for cosmetics and tea are used, and the leaves of Geumhwagyu also contain various physiologically active substances, so they are used for various purposes.

In the present invention, the cosmetic composition is prepared using the leaves of Keumhwagyu as described above as an active ingredient, and it is based on extracting the leaves of Keumhwagyu using benzyl glycol. The leaves of Geumhwagyu that can be used at this time can be used from new leaves sprouting in spring to fallen leaves in autumn.

In addition, the paw paw tree is a deciduous broad-leaved tree that is native to the United States and distributed from the Atlantic coast to New York State in the north, and Michigan and Kansas in the west, flowering is around April, and the fruit is harvested in September to October, and the fruit 1 A dog weighs 50-300g and it is a banana. pineapple. It has a refreshing taste similar to that of mango and has a sugar content of 25 blix or more. It is a plant with the strongest anti-cancer properties on the planet. The American medical community is designating it as a food species with the best development potential as a future health food for mankind. .

In addition, the paw paw tree grows up to 12 m in height, and the drooping leaves are wide and the length of the long oval leaves is sharp and reaches 30 cm. The stinky 5cm purple flower blooms before the leaves appear in the spring, and the 8~18cm long edible fruit resembles a short and thick banana, but the skin turns red as the fruit matures. Fruits vary in size, maturity, and taste depending on the variety, and some people experience skin reactions when they touch the fruit of this tree. Seven other species of the genus Asimina are shrub-like plants native to North America, including A speciosa and A angustifolia, both of which are large, yellow or white. It is known that, according to reports, the tree with the most powerful anti-cancer properties among all the plants that have been discovered on the earth so far is a paw paw tree.

In particular, according to the data reported to the pharmaceutical industry, the plants containing the most anticancer ingredients are "Taxol" extracted from "Graviola" and "Yu-mok", and it was found that Paw paw tree has 300 times more. In particular, the active ingredient of paw paw tree reduces the cell's ATP production, thereby lowering the voltage within the cell, whereas normal cells have enough ATP, so there is no problem, but cancer cells are normal because they produce energy through fermentation. It has been found that 10-17 times more energy than cells are required, and thus, the voltage is greatly reduced due to a relatively large influence, causing the cancer cells to disintegrate and have an anticancer effect. The paw paw tree does not necessarily only act on cancer cells, and if there are cells that use a relatively large amount of energy in the human body, they unconditionally run to it and cut off the energy supply. For that reason, the paw paw tree completely removes the parasites in the body and even wipes out the lice on the hair. In addition, there are experimental results that paw paw tree shrinks tumors in prostate cancer patients and reduces the level of prostate-specific antigen, that is, PSA, and there are also research results that have a good effect on lymphoma and decrease the number of white blood cells and lymphocytes in non-Hodgkins patients. .

In the present invention, the cosmetic composition is prepared as an active ingredient using the leaves of the paw paw tree as described above, and it is based on extracting the paw paw tree leaves using benzyl glycol. The leaves of the paw paw tree that can be used at this time can be used from new leaves sprouting in spring to deciduous leaves in autumn.

As described above, the benzyl glycol extract of Geumhwagyu leaf and the benzyl glycol extract of paw paw tree leaves are each preferably contained in an amount of 0.001 to 20% by weight based on the total weight of the composition, and if it is less, the effect can be exerted, and in excess When included, the effect increase is no longer made, and it is undesirable because it may affect the stability of the formulation.

The composition according to the present invention may further include a benzyl glycol extract from a pure vegetable leaf in order to further enhance the effect of the extract of Geumhwagyu and paw paw tree, and preferably, the composition according to the present invention comprises a benzyl glycol extract from a pure vegetable leaf. 0.001 to 20% by weight based on the total weight of the composition.

Perennial herbs are perennial plants of the dicotyledonous plant, Buttercylaceae, and its scientific name is Brasenia. schreberi , which grows in ponds, but was also cultivated in paddy fields to eat leaves and shoots in the past. The leaves are alternate phyllotaxis and oval, the back side is purple, and the petiole hangs in the center. When the leaves grow, they are surrounded by agar-like mucus along with the young stems. Flowers bloom from May to August, are black, reddish purple, and hang one by one at the end of a long peduncle that grows from the leaf axil, and is about 2cm in diameter. There are three sepals and three petals each. There are 6-18 pistils, there are nipple-shaped projections, and there are many stamens. The stamen surrounds the pistil and is longer than the pistil, and the central pistil is curved outward. After the operation withers, the pistil grows and a sticky slime is formed on the head.

As described above, the cosmetic composition in the present invention may include components commonly used in cosmetic compositions, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers. include

Such a cosmetic composition may be prepared in any formulation conventionally prepared in the art. In one embodiment, it may be any one formulation selected from the group consisting of a high-viscosity emulsifier type, a low-viscosity emulsifier type and a solubilizing formulation, but is not limited thereto. According to the formulation to be manufactured, it may include a cosmetic composition formulation component commonly used in the technical field to which the invention pertains. For example, to be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, and spray. However, the present invention is not limited thereto. More specifically, flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, sun lotion, sun cream, makeup base, BB cream, cleansing cream, cleansing foam, cleansing water, pack, stick product, balm ( Balm) type product, spray or powder formulation.

Depending on the formulation to be manufactured, an aqueous phase component, an oil phase component, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a preservative, a fragrance, etc. may be selected and added.

Hereinafter, the present invention will be described in detail through Examples and Experimental Examples. It is clear that the present invention is not limited by the description of the above-described embodiments.

< Example 1: Preparation of the composition according to the present invention>

500 g each of crushed Keumhwagyu leaves and pawpaw tree leaves were mixed with 1 liter of benzyl glycol.

The mixed raw materials were immersed and extracted for 7 days at room temperature, and then filtered with a 250 mesh and 0.5 μm filter paper to obtain a liquid extract. The obtained liquid extract was concentrated to prepare a composition according to the present invention.

< Example 2: Preparation of the composition according to the present invention>

A composition according to the present invention was prepared in the same manner as in Example 1, except that 500 g of crushed pure green leaves were further included.

< comparative example 1: Preparation of ethanol extract>

Same as Example 1, except that ethanol was used instead of benzyl glycol.

< comparative example 2: Preparation of ethanol extract>

Same as Example 2, except that ethanol was used instead of benzyl glycol.

< comparative example 3: Geumhwagyu Preparation of single extract>

It is the same as in Example 1, except that it is extracted with benzyl glycol using only Keumhwagyu.

< comparative example 4: pawpaw tree Preparation of single extract>

It is the same as in Example 1, but there is a difference in extraction with benzyl glycol using only paw paw tree.

< comparative example 5: Preparation of pure vegetable alone extract>

It is the same as in Example 1, except that it is extracted with benzyl glycol using only pure vegetables.

< test example 1: Moisturize the skin>

1. Cytotoxicity experiments

The cytotoxicity test was performed using Moseman's method (Moseman TJ, Immuno Methods, 65, 55 (1983)) and Skaper et al. (Skaper SD, et al, Cell Culture, 2, 17 (1990)) MTT (3-4). ,5-dimethylthiazol-2yl)-2,5-diphenyl-2H-tetrazolium bromide) was measured.

Human keratinocytes HaCaT were purchased from the Korean Cell Line Bank (Seoul, Korea) and used. HaCaT was cultured in an animal cell incubator at 37°C and 5% CO2 supply condition in a laboratory using DMEM medium containing 10% (V/V) FBS, penicillin 100U/mL, and streptomycin 100g/mL antibiotic.

The compositions of Examples and Comparative Examples were dissolved in DMSO and added to the culture medium at 100 g/mL, and the cells were cultured in the culture medium for 24 hours. After treatment with MTT solution (5 mg/mL) and further incubated for 4 hours, the amount of the produced blue crystal formazan was measured for absorbance at 595 nm using an ELISA reader. Toxicity to cells was expressed as a percentage of the average absorbance value of each control group.

To investigate cytotoxicity in HaCaT keratinocytes, when treated at a concentration of 100 g/mL, cytotoxicity was investigated by calculating the degree of change in cell proliferation compared to that treated only with DMSO, a control.

In all the results, cell viability showed a result of about 98%, and it was found that both the compositions of Examples and Comparative Examples had no cytotoxicity.

2. Aquaporin -3 and Involuclein Synthesis accelerating effect experiment

In order to confirm the effect of increasing the synthesis of involucrin, which plays an important role in the barrier and moisturizing functions of skin cells, and aquaporin-3, which plays an important role in water movement, the compositions of Examples and Comparative Examples were applied to the cells as follows. After each treatment, it was confirmed by western blotting.

First, human keratinocytes were inoculated with HaCaT, and after 24 hours, the compositions of Examples and Comparative Examples were added, respectively, and cultured in DMEM medium, 37° C., 5% CO 2 . After culturing for 24 hours, the cells were recovered, disrupted, and only proteins were extracted and stored at -40°C. The extracted protein was quantified by BCA saasy. After dilution with 30 g of protein amount of each sample, electrophoresis on 12% SDS-gel was carried out, and then it was transferred to PVDF membrane. After reacting with the primary antibody (rabbit anti-aquaporins-3 antibody), the remaining antibody was washed and removed and reacted with the secondary antibody (rabbit HRP-coupled anti-IgG antibody).

After removing the remaining antibody, a reagent (peroxidase substrate and chromogen solution) that reacts with the antibody to cause a color reaction was added. The colored band was measured and quantified through image analysis (BioRad, Universal Hood, USA), and the results are shown in Tables 1 and 2 below.

division Aquaporin-3 expression level (%) control 100 Example 1 151 Example 2 155 Comparative Example 1 119 Comparative Example 2 121 Comparative Example 3 113 Comparative Example 4 105 Comparative Example 5 113

As shown in Table 1, Examples 1 and 2 according to the present invention showed a synergistic effect of about 50%, showing a more remarkable effect than Comparative Examples 1 and 2.

division Expression level of involucrin (%) control 100 Example 1 149 Example 2 151 Comparative Example 1 122 Comparative Example 2 123 Comparative Example 3 114 Comparative Example 4 109 Comparative Example 5 116

As shown in Table 2 above, the expression of involucrin, which plays an important role in skin barrier function and moisturizing, when treated with HaCaT keratinocytes, showed a synergistic effect of about 50% between Examples 1 and 2 according to the present invention. It showed a more remarkable effect than the examples.

Through the above experimental results, the composition of the present invention was able to confirm the excellent effect on the skin barrier function and moisturizing.

< test example 2: Prevent wrinkles and maintain elasticity>

One. Elastase Inhibition rate measurement

The inhibitory effect on the activity of Elastase, an enzyme decomposing elastin, was confirmed as follows.

Elastase used elastase derived from human leukocyte cells, and a synthetic substrate MeOSuc-Ala-Ala-Pro-Val-pNA was used as a substrate for elastase. A buffer solution of 100 mM Tris (pH 7.5) was used. Elastase was finally used at 0.2 mU using a buffer solution. In addition, the synthetic substrate of elastase was diluted with a buffer solution to a final concentration of 0.5 mM after making a 100 mM solution using DMSO.

At this time, the positive control was set to put quercetin (Quercetin) known as an elastase inhibitor at a concentration of 100ppm. Samples of Examples and Comparative Examples were also added so as to be 100 ppm. The reaction was carried out in a 96-well plate, and reacted at room temperature for 20 minutes. The absorbance was measured at 405 nm at 1-minute intervals using a spectrophotometer, and the slope of the absorbance versus time was determined to determine the activity of the enzyme. Elastase inhibition was calculated as follows and the results are shown in Table 3.

sample Elastase inhibition rate (%) positive control 43.12% Example 1 75.12% Example 2 77.15% Comparative Example 1 40.18% Comparative Example 2 41.36% Comparative Example 3 41.36% Comparative Example 4 38.15% Comparative Example 5 40.19%

As can be seen from the results of Table 3, it was found that the composition of the present invention had a very good elastase inhibitory ability compared to the comparative example, and in particular, the composition of the present invention was significantly superior to those of Comparative Examples 1 and 2 by extracting benzyl glycol. It may be the effect of using it as a solvent.

2. Collagenase Inhibition rate measurement

The inhibitory effect on the activity of collagenase, an enzyme that decomposes collagen, was confirmed as follows.

Collagenase derived from mouse pancreas was used, and a synthetic substrate, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala, was used as the collagenase substrate. A buffer solution in which 50 mM Tricine, 10 mM CaCl ₂ ·H ₂ O, and 400 mM NaCl (pH7.5) were dissolved was used. The synthetic substrate was dissolved at a concentration of 10 mM using a buffer and finally diluted to 75 μM. Collagenase was dissolved in 4 degree cold tertiary distilled water to a concentration of 1 unit/ml, and finally diluted with a buffer solution to 0.05 unit/ml.

Samples in Examples and Comparative Examples were tested at a final concentration of 100 ppm. At this time, the positive control group was set to have a final concentration of 100 ppm of epigallocatechin gallate (EGCG), which is known to have a collagenase inhibitory effect. The reaction was carried out in a 96-well plate, and reacted at room temperature for 10 minutes. The absorbance was measured at 345 nm at intervals of 1 minute using a spectrophotometer, and the maximum slope of the absorbance versus time was obtained and determined as the activity of the enzyme. The collagenase inhibition rate was calculated as follows and the results are shown in Table 4.

sample Collagenase inhibition rate (%) positive control 42.12% Example 1 63.32% Example 2 67.19% Comparative Example 1 40.12% Comparative Example 2 41.25% Comparative Example 3 35.18% Comparative Example 4 22.01% Comparative Example 5 31.25

As can be seen from the results in Table 4, it was found that the composition of the present invention has a very superior collagenase inhibitory ability than the comparative examples, and in particular, exhibiting a more remarkable effect than Comparative Examples 1 and 2 was obtained by using benzyl glycol as an extraction solvent. It will be the effect used as

As a result of the above examination, it was found that the cosmetic composition according to the present invention is excellent in maintaining and improving skin elasticity and preventing skin wrinkles, and is ultimately the best cosmetic composition capable of preventing skin aging.

In summary of the above-described test examples, the composition according to the present invention has remarkably excellent advantages in skin moisturizing, maintaining or improving skin elasticity, preventing skin wrinkles, and ultimately, skin anti-aging effect.

Claims (4)

Benzyl glycol extract from the leaves of Keumhwagyu; A cosmetic composition with excellent skin moisturizing effect by promoting the synthesis of aquaporin-3 and involucrin by containing benzyl glycol extract of paw paw tree leaf and benzyl glycol extract of pure vegetable leaf as active ingredients.
3. The method according to claim 1 or 2, further comprising: a benzyl glycol extract from the leaf of Keumhwagyu; A cosmetic composition comprising 0.001 to 20% by weight of each of the leaf benzyl glycol extract of paw paw paw tree and the benzyl glycol extract of pure vegetable leaves based on the total weight of the composition.

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