KR20120026308A - Cosmetic composition comprising algae stem cell extract as active ingredient - Google Patents

Abstract

PURPOSE: A cosmetic composition containing seaweed stem cell extract is provided to ensure antioxidation, cell proliferation, collagen synthesis and skin moisturizing. CONSTITUTION: A cosmetic composition for improving skin condition contains 0.00001-50 wt% of seaweed stem cell extract as an active ingredient. The seaweed includes Hizikia fusiformis, Undaria pinnatifida, Laminaria japonica, Eisenia bicyclis, Sargassum confusum, Gelidium amansii, Porphyra tenera, Porphyra yezoensis, Undaria pinnatifida, Enteromorpha, or Codium fragile. The stem cells are pluripotent stem cells or multipotent stem cells.

Description

Cosmetic composition containing algae stem cell extract as an active ingredient {Cosmetic Composition Comprising algae stem cell extract as active ingredient}

The present invention relates to a cosmetic composition containing seaweed stem cell extract as an active ingredient.

Skin is an important organ that protects the body from external stimuli on its surface and performs various functions such as thermoregulation, sensation, secretion, excretion, and vitamin D formation. However, as the skin ages, the function of the skin decreases, wrinkles increase, skin relaxation, senile pigmentation, skin elasticity, skin exfoliation, and skin glossiness appear. In addition, the skin aging is further accelerated by external environmental factors such as strong ultraviolet rays, stress, pollution, air conditioning and use of air conditioners and causes various skin diseases and skin damage, including atopic dermatitis.

The skin is composed of epidermis, dermis and subcutaneous tissue, and it is composed of appendages such as sebaceous gland, hair and Hanseon. The epidermis and dermis are directly related to aging and skin damage recovery.

The epidermis forms new keratinocytes through cell division, removes the aged keratin from the skin to maintain healthy skin, and the natural moisturizing factor keeps the skin supple and elastic.

The dermis is connected to the basement layer of the epidermis and is 10-40 times the thickness of the epidermis. The dermis nourishes the epidermis, protects the body from external damage, regulates body temperature, senses, and regenerates the skin through interaction with the epidermis and holds 60% of the skin's moisture content. The dermis is predominantly composed of collagen, elastin and matrix-forming fibroblasts. Collagen and elastin play an important role in maintaining the elastic balance of the skin and maintaining skin radiance and tension.

In general, aging of the skin is caused by a decrease in the function of the epidermis and the dermis. In particular, it appears as a decrease in the epidermis, delayed exfoliation of keratinocytes and a decrease in the collagen and elastin of the dermis. In addition, cell membrane oxidation by free radicals generated from respiration and external stimuli also promotes skin aging. Therefore, in order to prevent skin aging, it is necessary to moisturize, promote fibroblast activity, promote collagen production, inhibit collagenase, and antioxidant activity.

Various physiological studies and cosmetic researches for preventing skin aging, preventing skin damage, promoting skin regeneration, and research on cosmetics have been carried out to promote exfoliation of retinoids and epidermal layers such as retinol and retinoic acid, which have wrinkle and elasticity improvement effects. Adenosine and the like have been developed. However, all of them show irritation to the skin and have a problem in that they are easily oxidized or deteriorated due to poor stability. Therefore, there has been a need for a safer skin and a stable ingredient as a cosmetic.

On the other hand, stem cells are undifferentiated cells and cells that are the basis of cells or tissues that make up an individual, and are capable of self-producing and have a multi-differentiation ability to differentiate into cells having specific functions according to the environment. Stem cells are divided into pluripotent stem cells, multifunctional stem cells, and redundant stem cells according to their differentiation capacity. Pluripotent stem cells are cells that can develop into a single complete individual, and multifunctional stem cells cannot be individual but various organs Stem cells that can differentiate into and redundant stem cells are stem cells that can differentiate into only one line.

Stem cells are known to promote the differentiation and division of cells by producing a variety of growth factors. Stem cells present in the skin are present in the basement layer of the epidermis 2-7% and is involved in regeneration that promotes cell division in case of skin damage. However, due to aging, the number and function of undifferentiated stem cells is reduced, and the regeneration of skin is lost.

Recently, interest in cosmetics using stem cells is increasing, and research on this is increasing. For example, a cosmetic composition containing adipose derived stem cell culture solution (Korea Patent Publication No. 10-2010-0036389) and a cosmetic composition containing adipose tissue-derived multipotent stem cell culture and its extracted protein (Korea Patent Publication No. 10-2010- 0008763). However, such animal, in particular, cosmetics using human stem cells has been raised problems due to the lack of safety, such as infection and negative recognition of the use of human stem cells.

In order to reduce the problems of animal stem cells, research on plant stem cells is continued, and a cosmetic composition (Korean Patent Publication No. 10-2010-0080370) containing mung bean stem cell culture products as an active ingredient and apple stem cells are used for preventing skin aging. Cosmetic compositions (Korean Patent Publication No. 10-2009-0108812), Azuga anti-aging cosmetic composition using the raptans stem cells (Korean Patent Publication No. 10-2009-0104223) and the like have been developed. However, no cosmetics using algae stem cells have been studied.

Therefore, the present inventors searched various seaweeds to find a suitable ingredient for skin improvement cosmetics from the stem cells of algae, and as a result, the stem cell extract of sesame seed has excellent antioxidative activity, cell proliferation effect, collagen synthesis effect and collagenase activity inhibitory effect. It has been found that the cosmetic composition containing the anti-aging effect has an excellent moisturizing effect and skin soothing effect.

In addition, the cosmetics of the present invention was found to be safe for the skin as well as excellent stability as a natural product because there is no irritation.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of cited papers and patent documents are incorporated herein by reference in their entirety, and the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

The present inventors endeavored to find a suitable ingredient for skin improvement cosmetics from the stem cells of algae, and as a result, the stem cell extract of seaweed, Sesame seaweed, has antioxidative activity, cell proliferation effect, collagen synthesis effect and collagenase activity inhibitory effect. Excellent regeneration and anti-aging effect and the cosmetic composition containing it was found to have excellent moisturizing effect and skin soothing effect.

In addition, the cosmetic of the present invention has been completed by confirming that the natural product has no irritation and is not only safe for the skin but also excellent in cosmetic stability.

Therefore, an object of the present invention is to provide a cosmetic composition for improving skin condition containing stem cell extract of seaweed sesame as an active ingredient.

Other objects and advantages of the present invention will become apparent from the following detailed description, claims and drawings.

The present invention provides a cosmetic composition for improving skin condition containing algae stem cell extract.

The present inventors earnestly researched to find a suitable component as a skin improvement cosmetics from the stem cells of seaweed. As a result, the cosmetic composition containing the stem cell extract of sesame seed having the above-mentioned advantages was completed, and the stem cell extract had antioxidant activity, cell proliferation effect, collagen synthesis effect, and collagenase activity inhibitory effect, thereby preventing skin regeneration and aging. Excellent effect, it was confirmed that the cosmetic composition containing it exhibits an excellent moisturizing effect and soothing effect.

According to one aspect of the invention the present invention provides a cosmetic composition for improving the skin condition containing the algae stem cell extract as an active ingredient.

According to a preferred embodiment of the present invention, the seaweeds used in the present invention are preferably Hizikia fusiformis , seaweed ( Undaria pinnatifida ), kelp ( Laminaria japonica ), rhubarb ( Eisenia bicyclis ), Sarbansum ( Sagassum confusum ) ( Gelidium amansii ), sesame ( Porphyra tenera ), sesame ( Porphyra yezoensis ), green seaweed ( Undaria pinnatifida ), green seaweed (Enteromorpha) and hearing ( Codium fragile ) can be used, more preferably sesame seed ( Porphyra tenera ), Porphyra yezoensis , Undaria pinnatifida may be used, and most preferably Porphyra tenera may be used.

Stem cells of the algae used in the present invention may preferably be used as omnipotent stem cells, multifunctional stem cells and redundant stem cells, and most preferably can be used as omnipotent stem cells.

As an embodiment of the present invention, the sesame seed (Porphyra tenera) is a seaweed algae of red algae plant and purple seaweed, and the stem cells of sesame seaweed are chochucelis, a filamentous body of sesame seed, and are omnipotent to differentiate into one individual like human fertilized egg. Has Sesame stem cells can be cultured on a liquid medium containing seawater, and can be differentiated into one complete individual due to changes in light conditions and composition of the culture medium.

When culturing the algae stem cells in a liquid phase, preferably a photobioreactor may be used.

The photobioreactor is a microalgae culturing apparatus, commonly referred to as a photobioreactor. Microalgae culturing device, like the characteristics of microalgae that photosynthesis, absorbs a lot of light energy by widening the surface area per volume of the reactor in the form of glass, plastic, vinyl bags, ponds, etc. It is designed for use by birds.

After the process of extracting the stem cells cultured in the above, the algae stem cell extract of the present invention can be extracted by using a known method of the stem cells of algae, such a known method in the chemical method (eg For example, extraction using a solvent such as ethanol) and extraction using a physical method (for example, ultrasonic or compression) may be used, but are not limited thereto.

According to a preferred embodiment of the present invention, in the cosmetic composition containing the seaweed stem cell extract of the present invention as an active ingredient, the seaweed stem cell extract is preferably contained in 0.00001% to 50% by weight, more preferably 1% by weight % To 20% by weight, even more preferably 3% to 8% by weight, most preferably 5% by weight. When the seaweed stem cell extract is contained in 0.00001% by weight or less, the effect is insignificant, and when contained in more than 50% by weight, the effect does not increase significantly.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to seaweed stem cells as active ingredients, and include conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and flavors, and carriers. Include.

The cosmetic composition for protecting the skin of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants- Containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays and the like, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Skin condition improvement effect of the present invention means antioxidant activity, cell activity promoting effect, collagen synthesis effect and collagenase activity inhibitory effect and moisturizing and skin soothing effect, but is not limited thereto.

The features and advantages of the present invention are summarized as follows:

(Iii) The present invention provides a cosmetic composition for improving skin condition using the algae stem cell extract as an active ingredient.

(Ii) The seaweed stem cell extract of the present invention and the cosmetic containing it as an active ingredient are excellent in antioxidant activity, cell proliferation effect, collagen synthesis effect and collagenase activity inhibitory effect, has an excellent moisturizing effect and skin soothing effect.

(Iii) In addition, the cosmetics having an effect of improving the skin condition of the present invention contains an active ingredient obtained from stem cells of algae, which is safe for the skin and has an excellent stability as a cosmetic.

Figure 1 shows the collagen synthesis increase rate of sesame seed stem cell extract.
Figure 2 shows the collagenase (MMP-1) activity (%) of sesame stem cell extract.
Figure 3 shows the moisturizing nutrient lotion containing sesame seed stem cell extract.
Figure 4 shows the moisturizing improvement rate of nutrient lotion containing sesame seed stem cell extract.
5 is a photograph confirming the skin soothing effect of sesame stem cell extract.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

Preparation Example 1: Preparation of sesame seed stem cell extract (Preparation Example 1) and sesame seed extract (Comparative Preparation Example 1)

Sesame seed stem cells (almighty stem cells) used in Preparation Example 1 were distributed by Jeollanam-do Marine Bio Research Institute and incubated in a photobioreactor (manufactured by the company) at 20 ° C and 800 Lux for 30 days, followed by 0.2 mm Whatman filter paper (No 5 g of Watman paper was removed to remove all of the medium and 20 g of the cultured 500 g of the cultured cells washed with distilled water at 4-8 ° C.

In Comparative Preparation Example 1, 500 g of sesame seed cultured on the shore near Jindo was washed in distilled water, and sesame seed extract was prepared by the method described in Preparation Example 1.

Preparation Example 2 Preparation of Nutritional Lotion Containing Sesame Stem Cell Extract

The nutritional lotion (Examples 1 to 3) containing 1.0, 3.0, and 5.0% by weight of the sesame seed stem cell extract prepared in Preparation Example 1, respectively, according to the ingredients and compositions shown in Table 1 below, and a conventional lotion without the same (Comparative Example 1) and a lotion (each Comparative Example 2) containing sesame seed extract prepared in Comparative Preparation Example 1 were prepared.

Specific manufacturing methods are as follows:

According to the method conventionally used in the art, components 10, 11, 13, and 16 were sequentially added and dissolved by heating to 80 ° C, and components 2 to 9 and 12 were dissolved. Thereafter, the mixture was cooled to 50 ° C., ingredients 14 to 15 were mixed, cooled to room temperature, and the ingredients 1 of the present invention were added to complete the nutrition lotion.

Comparative Example 2 was prepared by adding component 17 instead of Comparative Example 1 and Preparation Example 1, in which Example 1 was not added.

number Raw material Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 One Preparation Example 1 - - 1.0 3.0 5.0 2 Beeswax 2.0 2.0 2.0 2.0 2.0 3 Polysorbate 60 2.0 2.0 2.0 2.0 2.0 4 Solbitan Sesquioleate 2.0 2.0 2.0 2.0 2.0 5 Liquid paraffin 0.5 0.5 0.5 0.5 0.5 6 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 7 Lipophilic Monostearic Acid Glycerin 1.0 1.0 1.0 1.0 1.0 8 Stearic acid 1.0 1.0 1.0 1.0 1.0 9 Glyceryl Stearate / Pig-400 Stearate 4.0 4.0 4.0 4.0 4.0 10 Propylene glycol 4.0 4.0 4.0 4.0 4.0 11 Carboxy Polymer 4.0 4.0 4.0 4.0 4.0 12 Triethanolamine 0.3 0.3 0.3 0.3 0.3 13 antiseptic 5.0 5.0 5.0 5.0 5.0 14 Pigment 3.0 3.0 3.0 3.0 3.0 15 Spices 0.5 0.5 0.5 0.5 0.5 16 Purified water Balance Balance Balance Balance Balance 17 Comparative Preparation Example 1 - 3.0

Unit: weight%

Experimental Example  One: Patience  Antioxidant Effect of Stem Cell Extracts

Electron donating ability (electron donating ability using DPPH (2,2-Diphenyl-1-picrylhydrazyl, Sigma) method for the sesame seed stem cell extract prepared in Preparation Example 1 as a measure of oxidation inhibition by donating electrons to free radicals If the electron donating ability to DPPH is large, high antioxidant activity and scavenging action against other radicals including active oxygen can be expected). The sesame seed stem cell extract of Preparation Example 1 and the sesame seed extract of Comparative Preparation Example 1 were used as shown in Table 1 below, diluted with 0.1% ascorbic acid, an antioxidant commonly used in the art. Liquid and free radical scavenging ability were compared.

Sesame Stem Cell Extract Sesame Extract 0.1% - 1.0% - 0.1% - 1.0%

2 ml of the sesame stem cell extract dilution of Table 2 was added to 1 ml of 0.2 mM DPPH ethanol solution, and the mixture was mixed well at room temperature for 10 seconds and then reacted at 37 ° C. for 30 minutes. And the absorbance was measured using a UV / VIS spectrophotometer (UV / VIS Spectrophotometer, Humacoperation, Korea) at 517 nm and the electron donating ability calculated according to the following Equation 1 is shown in Table 3 and FIG.

Free radical scavenging ability (%) Ascorbic acid Production Example 1
(0.1%) Production Example 1
(1.0%) Comparative Production Example 1
(0.1%) Comparative Production Example 1
(1.0%) 88% 87% 95% 71% 80%

As shown in Table 3, the sesame stem cell extract diluent of the present invention exhibited a very high electron donating ability, showing an excellent effect than the general sesame extract of Comparative Preparation Example 1. In addition, sesame seed stem cell extract showed a strong antioxidant effect by showing an electron donating ability similar to ascorbic acid, a powerful antioxidant at the same concentration.

Experimental Example  2: Patience  Confirmation of Cell Proliferation Effect of Stem Cell Extract

The cell proliferation effect using cell culture was measured for the sesame stem cell extract prepared in Preparation Example 1. Cell proliferation tests are described in Mosmann T. et al. Rapid Colorimetric Assay for Cellular Growth and Survival : Application to Proliferation and Cytotoxicity Assays, J. Immunol. Meth., 65: 55-63 (1983)). The medium used for cell proliferation and toxicity test was a human fibroblast cell line (KCLB, CCD-986) in Dulbeco's Modified Eagle's Medium, Gibco (DMEM) containing 10% FBS (fetal bovine serum, Gibco). The well plates were used inoculated with 1 × 10 ⁴ cells / ml of water. After 24 hours of inoculation, the medium was replaced with no FBS, and the sesame stem cell extract of Preparation Example 1 was added at different concentrations, followed by 3 days of 37 ° C. and 5% CO ^2. The culture was carried out in an incubator, and only the control medium was added to the control group. After incubation, 1 ml / well of MTT [3- (4,5-Dimethylthiazol-2-yl) -2,5-Diphenyltetrazolium Bromide, Dojindo Laboratories ZY903) solution was added, and after 4 hours at 37 ° C. in a CO ² incubator. After removing the medium and adding 200 μl of DMSO (dimethylsulfoxide, Sigma D 4540) to each, and measuring absorbance at 550 nm, the cell proliferation rate was calculated according to the following Equation 2 and shown in Table 4 below.

Extract concentration (%) Cell proliferation rate (%) Preparation Example 1 Comparative Production Example 1 0 (control) 100 100 0.01 100 100 0.03 108 105 0.05 114.13 110 0.1 120 102 0.15 121 98.6 0.3 131 95.2

In Table 4, when the cell proliferation (control) at the optimum conditions for cell growth is 100%, the sesame stem cell extract of Preparation Example 1 of the present invention can be seen that the cell proliferation ability and the general sesame extract of Comparative Preparation Example 1 It can be confirmed that the sesame stem cell extract of the present invention has no toxicity and exhibits cytotoxicity at 0.15% or more, and the higher the treatment concentration, the higher the cell proliferation ability.

Experimental Example  4: Patience  Confirmation of Collagen Synthesis Enhancement Effect of Stem Cell Extracts

In order to examine the collagen synthesis enhancement effect of the sesame seed stem cell extract of Preparation Example 1 inoculated to 5 × 10 ⁴ human fibroblast cell line (KCLB, CCD-986) in a 24-well plate 37 ℃, 5% CO After 24 hours of incubation at ² and exchanged with a medium containing 0.05% FBS, and treated with Preparation Example 1 and Comparative Preparation Example 1 at a concentration of 0.1% that does not show cytotoxicity, and cultured for 48 hours and collected each culture medium microplate The amount of collagen was measured in a well (microplate well) using a Type-I procollagen Kit (Takara, Japan).

   Biosynthesis growth rate is calculated according to the following equation 3 and shown in Table 5.

Collagen Synthesis Growth Rate (%) Ascorbic acid Preparation Example 1 Comparative Preparation Example 1 48% 50% 37%

Test results showed that the sesame stem cell extract of the present invention showed a collagen synthesis promoting effect of 50% and higher collagen synthesis promoting effect than the normal sesame extract (Fig. 1). In addition, it can be seen that the sesame seed stem cell extract of the present invention has a very good collagen synthesis promoting effect by showing an enhancement effect similar to ascorbic acid known to have an excellent collagen synthesis promoting effect.

Experimental Example 5: Inhibitory Effect of Sesame Stem Cell Extract on Collagen Degrading Enzyme Activity

In order to examine the collagen synthesis enhancement effect of the sesame seed stem cell extract of Preparation Example 1 inoculated to 5 × 10 ⁴ human fibroblast cell line (KCLB, CCD-986) in a 24-well plate 37 ℃, 5% CO After 24 hours of incubation under ² , exchanged with a medium containing 0.05% FBS, and treated with Preparation Example 1 and Comparative Example 1 at a concentration of 0.1% which does not exhibit cytotoxicity. In a well, MMP-1 standard solution (Takara Bio Inc. Japan) and a quantitative buffer solution (Takara Bio Inc. Japan) were added and incubated at room temperature for 2 hours. After incubation, the cells were washed with a washing buffer, peroxidase conjugate solution (Peroxidase conjugate solution, Takara Bio Inc. Japan) was added and reacted and washed with PBS. After washing, TMB substrate (3,3 ', 5,5'-tetramethylbenzidine, Takara Bio Inc. Japan) was added and reacted at room temperature for 30 minutes, and then 1M sulfuric acid was added to measure absorbance at 450 nm.

Test results showed that the sesame stem cells of the present invention inhibit the activity of the collagenase MMP-1 and showed a superior effect than the normal sesame extract, the results are shown in Table 6. In addition, at the same concentration, it showed an effect similar to that of asiaticoside, which is known to have an excellent inhibitor of collagenase.

Sample (0.1%) Collagenase (MMP-1) activity (%) Negative Control (No Sample) 100 Positive control (asiaticoside) 80 Preparation Example 1 86 Comparative Preparation Example 1 92

Experimental Example  6: Patience  Moisturizing effect on the skin of the lotion containing the stem cell extract, safety test on the skin, cosmetic property stability test

Experimental Example  6-1: Moisturizing effect on skin

Moisturizing effect was measured by applying the nutrition lotions of Examples 1 to 3 and Comparative Examples 1 to 2 of Table 1 to the human body. After applying the Examples 1 to 3 and Apostasy Examples 1 to 2 to the test subjects, the moisturizing power was measured using a moisture meter (Corneometer, CK electronics, Germany). The specific measurement method is to clean the test site of 10 subjects, wait 30 minutes, and use the measured value as the baseline. After 30 minutes, 1 hour, 2 hours, 4 hours, and 8 hours after application of the sample, The moisturizing degree and the moisturizing induction map were confirmed and shown in Table 7 and FIG. 3, and the moisturizing improvement rate was obtained according to Equation 4 shown in Table 8 and FIG. 4.

Moisturizing Base 30 minutes 1 hours 2 hours 4 hours 8 hours Example 1 31.3 51.0 52.7 48.0 47.0 46.8 Example 2 31.5 52.3 53.2 53.5 49.9 48.2 Example 3 32.0 50.3 54.2 53.5 49.9 51.2 Comparative Example 1 28.6 35.1 38.3 38.5 36.4 36.2 Comparative Example 2 30.3 50.4 51.7 49.1 44.1 42.8

Moisturizing improvement rate 30 minutes 1 hours 2 hours 4 hours 8 hours Example 1 62.9 68.4 53.4 50.2 49.5 Example 2 66.0 68.9 69.8 58.4 53.0 Example 3 57.2 69.4 67.2 55.9 60.0 Comparative Example 1 22.7 33.9 34.6 27.3 26.6 Comparative Example 2 66.3 70.6 62.0 45.5 41.3

As a result, it can be seen that Examples 1 to 3 containing the sesame stem cell extract of the present invention exhibited high moisturizing and moisturizing retention with respect to Comparative Examples 1 and 2, and the higher the concentration, the higher the moisturizing retention. Could know. In addition, it can be seen that Examples 1 to 3 maintain very high moisturizing improvement rate of 49% or more after 8 hours, and show very good moisturizing power.

Experimental Example  6-2: Soothing effect

There was no hypersensitivity reaction to skin irritation, and 10 adult healthy men and women without skin disease or skin allergy symptoms were closed with a closed patch on the lower forearm of the arm to determine skin irritation after 48 hours. The skin reaction was determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG), and the test substance was prepared in Preparation Example 1 and Comparative Preparation Example 1.

The results are shown in Table 9 below, and the specific pictures capable of proving this are shown in FIG. 5.

As can be seen from Table 9 and FIG. 5, the sesame stem cell extract of Preparation Example 1 and Comparative Preparation Example 1 was found to have a skin irritation-releasing effect, and from these results, it was found that there was a calming effect on skin irritation. .

number division Skin irritation
(48 hours) One SLS (Control) 3 2 SLS + Comparative Production Example 1 2 3 SLS + Production Example 1 0

0: No irritation 1: Minimal irritation 2: Mild irritation (erythema) 3: Severe irritation (erythema, edema)

Experimental Example  6-3: In cosmetics  For stability test

Preservation stability as cosmetics for Examples 1 to 3 containing 1 to 5% of sesame stem cell extract of the present invention and Comparative Example 1 containing no extract, and Comparative Example 2 containing a general sesame extract In order to evaluate the properties of the properties according to the change of temperature for 3 months, the results are shown in Table 10.

division Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 -5 ℃ 1 week, 1 month, 3 months ◎◎◎ ◎◎◎ ◎◎◎ ◎◎◎ ◎◎◎ 4 ℃ ◎◎◎ ◎◎◎ ◎◎◎ ◎◎◎ ◎◎◎ Room temperature ◎◎◎ ◎◎◎ ◎◎◎ ◎◎◎ ◎◎ ○ 45 ° C ◎◎ ○ ◎◎ ○ ◎◎ ○ ◎◎ ○ ◎ ○ × ◎: Very good ○: Good ×: Discoloration, odor, phase separation

As a result of evaluating the stability of cosmetic properties, Comparative Example 2 containing general sesame extract showed phase separation and discoloration when stored at 45 ° C. for at least 3 months, whereas it was not stable as a cosmetic, whereas Comparative Example 1 of Examples 1 to 3 of the present invention. It was confirmed that it is stable as a cosmetic because there is no discoloration, odor and phase separation.

Claims (8)

Cosmetic composition for improving skin condition containing algae stem cell extract as an active ingredient.
The method of claim 1, wherein the seaweed is Hizikia fusiformis ), seaweed ( Undaria pinnatifida ), Laminaria japonica ), Rhubarb ( Eisenia bicyclis , Sargassum confusum ), Gelidium amansii ), sesame ( Porphyra tenera), Seaweed (Porphyra yezoensis), Parlay Kim (Undaria pinnatifida), green laver (Enteromorpha) and hearing (Codium fragile ) cosmetic composition, characterized in that it is selected from the group consisting of.
The method according to claim 2, wherein the seaweed is Porphyra tenera), Seaweed (Porphyra yezoensis ) and blue seaweed ( Undaria pinnatifida ) cosmetic composition characterized in that it is selected from the group consisting of.
The cosmetic composition according to claim 3, wherein the seaweed is Porphyra tenera .
According to claim 1, wherein the stem cells cosmetic composition, characterized in that selected from the group consisting of pluripotent stem cells and pluripotent stem cells.
The cosmetic composition according to claim 1, wherein the algae stem cells are cultured with a photobioreactor.
The cosmetic composition according to claim 1, wherein the skin condition improvement is for preventing skin aging, for preventing wrinkles, and for moisturizing the skin.
The cosmetic composition according to any one of claims 1 to 7, wherein the seaweed stem cell extract is contained in 0.00001 to 50% by weight.

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