CN106726868A - A kind of preparation method of the China rose anthocyanidin for having whitening capability - Google Patents
A kind of preparation method of the China rose anthocyanidin for having whitening capability Download PDFInfo
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- CN106726868A CN106726868A CN201611026841.5A CN201611026841A CN106726868A CN 106726868 A CN106726868 A CN 106726868A CN 201611026841 A CN201611026841 A CN 201611026841A CN 106726868 A CN106726868 A CN 106726868A
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- anthocyanidin
- china rose
- rose
- china
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The invention discloses a kind of preparation method of the China rose anthocyanidin for having whitening capability, new fresh China rose is distilled with steam distillation, water is 4.5 5.5 with the ratio of flower:1, the water vapour after distillation obtains coolant through cooling, when the coolant weight for distilling out is when adding 34 times of Chinese rose petal weight, that is, to stop distillation；After steam distillation, distiller bottom China rose Liquid Residue boils filtering in 0.5 1.5 hours jointly with China rose in distiller, filtrate removes highly polar composition by the tubing strings of DIAION HP 20, it is concentrated in vacuo after collection efflux and is drained, it is freeze-dried again to obtain final product Chinese rose anthocyanidin, put 20 DEG C of ice storages and hide.Product of the present invention can suppress melanocyte generation melanin.
Description
Technical field
The present invention relates to cosmetic material field, more particularly to a kind of China rose anthocyanidin preparation side for having whitening capability
Method.
Background technology
China rose (scientific name：Rosa chinensis Jacq.)：Be referred to as spending middle queen, also known as " Chinese rose ", be it is evergreen,
Half evergreen short shrub, is generally Hong Se ﹐ or pink colour Si seasons Kai Hua ﹐, occasionally has white and Huang Se ﹐ also may be used as ornamental plant
As medicinal plant, also known as Chinese rose.There are three natural mutation, modern distance education system flower pattern is various, there is single-lobe and polyphyll, also Gao Xinjuan
The grace flower pattern such as side；Its is beautiful in colour, abundant, the monochrome, the also kind such as colour mixture, silver-colored side such as only red, powder is not yellow, white；It is most
Kind has fragrance.Chinese rose it is various in style, existing nearly ten thousand kinds in the world, China also has more than thousand kinds.
Natural florescence August is spent into large-scale to April next year, from inside to outside, in divergence form, there is strong fragrance, can be widely used for
Horticultural gardening and cut-flower.The strong adaptability of Chinese rose, resist cold, it is cold-resistant, though ground plant, it is potted plant, it is adaptable to beautify the yard, decorate
Gardens, arrange flower bed, plant colored hedge, flower stand, Chinese rose cultivation is easy, can make cut-flower, for doing bouquet and the various gailys decorated basket, China rose
Piece extractable essence, and can be used as medicine.Also there is preferably antimycotic and collaboration overriding resistance fungi activity.
The content of the invention
It is an object of the invention to provide a kind of China rose anthocyanidin preparation method for having whitening capability.
The technical scheme that the present invention is provided is as follows：
An object of the present invention, is to provide a kind of China rose anthocyanidin preparation method for having whitening capability, including such as
Lower step：New fresh China rose is distilled with steam distillation, water is 4.5-5.5 with the ratio of flower:1, the water vapour after distillation
Through cooling, coolant is obtained, when the coolant weight for distilling out is when adding 3-4 times of Chinese rose petal weight, that is, to stop distillation；With
After steam distillation, distiller bottom China rose Liquid Residue boils filtering in 0.5-1.5 hours jointly with China rose in distiller, filters
Liquid removes highly polar composition by DIAION HP-20 tubing strings, to collect be concentrated in vacuo after efflux and drains, then freeze-dried is
Chinese rose anthocyanidin is obtained, -20 DEG C of ice storages is put and is hidden.
Preferably, distilling new fresh China rose with steam distillation, water is 5 with the ratio of flower:1, with steam distillation
Through cooling down the coolant of gained, when the coolant weight for distilling out is when adding 3-4 times of Chinese rose petal weight, that is, to stop steaming
Evaporate；After steam distillation, distiller bottom China rose Liquid Residue boils filtering in 1 hour jointly with China rose in distiller, filters
Liquid removes highly polar composition by configure the DIAION HP-20 tubing strings for completing, and is concentrated in vacuo after collection efflux and drained, then
Obtain final product Chinese rose anthocyanidin within freeze-dried 24 hours, put -20 DEG C of ice storages and hide.
It is still a further object of the present invention is to provide a kind of purposes of China rose anthocyanidin, it is used to suppress DPPH free radicals.
It is still a further object of the present invention is to provide a kind of purposes of China rose anthocyanidin, it is used to suppress tyraminase
Activity.
It is still a further object of the present invention is to provide a kind of purposes of China rose anthocyanidin, for suppressing melanocyte life
Into melanin.
Seen from the above description, the invention provides a kind of China rose anthocyanidin preparation method for having whitening capability, this hair
The China rose anthocyanidin of bright preparation, can be used to suppress DPPH free radicals, suppress tyrasamine enzymatic activity or suppress melanocyte
Generation melanin.And to human body skin without IRs such as redness, edema, papule, bubbles.
Brief description of the drawings
Fig. 1 suppresses the experimental result picture of DPPH free radicals for the season anthocyanidin of the embodiment of the present invention 5；Wherein abscissa is dense
Degree (ppm), ordinate is inhibiting rate (%)；
Fig. 2 is the patch tests result of the embodiment of the present invention 8；
Specific embodiment
Embodiment 1
Chinese rose anthosin dew, Chinese rose anthocyanidin production technology：
1. new fresh China rose (the bright red system's China rose of selection) is distilled with steam distillation, water is 5 with the ratio of flower:1, with
Water vapour after distillation obtains coolant through cooling, when 3 times that the coolant weight for distilling out is addition Chinese rose petal weight,
Stop distillation, this is Chinese rose essence dew, containing natural Chinese rose fragrance, can be used as natural Chinese rose water.
2. with steam distillation after, it is small that China rose Liquid Residue and the China rose in distiller of distiller bottom boil 1 jointly
When filter, filtrate removes highly polar composition by configure the DIAION HP-20 tubing strings for completing, and vacuum is dense after collection efflux
Contracting is drained, then freeze-dried 24 hours obtain final product Chinese rose anthocyanidin, is put -20 DEG C of ice storages and is hidden.
Total Flavonoid Content of the Chinese rose anthocyanidin of embodiment 2 is determined
Flavonoids in the yellow misfit thing that can form stabilization under alkalescence condition with aluminium chloride, and in wavelength 415nm
Under have extinction, when color is deeper, its Flavonoid Content is higher.Take one 96 porose discs and add sample or various concentrations standard items 20mL again
Sequentially add 60mL ethanol (95%), the aluminium chloride of 4mL 10% (aluminum chloride), 4mL 1M potassium acetates
(potassium acetate) and 112mL deionized waters, lucifuge reaction 30 minutes at room temperature, by ELISA reader in ripple
415nm long surveys its light absorption value.According to Flavonoid Content number in light absorption value discriminating test sample, previously system is substituted into using its light absorption value
Quercetin (quercetin) standard curve of work, makes and being represented relative to the concentration of Quercetin.Finally, every gram of sample is conversed
In contain how many milligram Flavonoid Content.
Total Flavonoid Content measurement result of Chinese rose anthocyanidin
| Sample | Total Flavonoid Content (mg Quercetin eq./g) |
| Chinese rose anthocyanidin extract | 26.6±2.0 |
The total phenol content of the Chinese rose anthocyanidin of embodiment 3 is determined
The measure of total phenol content as standard items, makes the gallic acid of various concentrations with gallic acid (gallic acid)
The calibration curve of light absorption value, the light absorption value of 750nm is surveyed using ELISA, and with light absorption value as the longitudinal axis, gallic acid concentration of standard solution is
Transverse axis draws standard curve, and calculates quantitative linearity regression formula.Light absorption value measured by sample is added by gallic acid calibration curve
After with conversion, you can obtain the content of the Determination of Polyphenols equivalent to how many gallic acids of testing sample, the amount of gallic acid
Higher, the antioxidation activity of representation compound is higher.
Gallic acid standard curve making：
(1) compound concentration is the gallic acid aqueous solution of 12.5,25,50,100,200 and 400 μ g/mL respectively.
(2) standard aqueous solution of the 50 above-mentioned concentration of μ L is respectively taken, 50 μ L forint phenol reagents (Folin-Ciocalteu's are added
Phenol reagent), uniform mixing is after being stored at room temperature 20 minutes.
(3) 100 μ L concentration are added for 20%Na2CO3The aqueous solution, is placed in room temperature reaction 10 minutes after mixing, determine in
The light absorption value (working as blank with water) of 750nm.
(4) it is the longitudinal axis using light absorption value, gallic acid concentration of standard solution is that transverse axis draws standard curve, and calculates quantitative
Linear regression formula.
The total phenol content of Chinese rose anthocyanidin is determined
| Sample | Total phenol content (mg Gallic acid eq./g) |
| Chinese rose anthocyanidin extract | 102.2±1.4 |
The Chinese rose anthocyanidin Cytotoxicity evaluation of embodiment 4 (Colorimetric MTT assay)
The moon is assessed from SCC25 cells (human tau neck squamous JEG-3) and HaCaT (human skin horn cell)
Season 72 hour cell toxicity test of anthocyanidin, as a result show that Chinese rose anthocyanidin 100mg/mL slightly has a little toxicity to SCC25 cells,
But cell survival degree is still above more than 70%；There is no toxicity to HaCaT (human skin horn cell) display anthocyanidin.
The Chinese rose anthocyanidin of embodiment 5 suppresses the assessment of DPPH free radicals
Method：Take 99 μ L DPPH ethanol solutions (100 μ L) mix 1 μ L extract after lucifuge react 30 minutes, most after
Light absorption value (BioTek, SynergyTM2, USA) is determined under wavelength 517nm.Compare with control group Vitamin C.
Result shows anthocyanidin when for 100ppm concentration, and display can reach 94.2% rejection ability, shows China rose
Green grass or young crops have the efficiency of very excellent suppression DPPH free radicals.
| Sample ID | Inhibiting rate (%) |
| Vit C (control group) | 97.7±0.9 |
| Anthocyanidin | 94.2±0.2 |
The Chinese rose anthocyanidin of embodiment 6 suppresses tyraminase Activity Assessment
In under concentration 100ppm, Chinese rose anthocyanidin shows the suppression Tyrosinase effect more than 50%.
| Sample ID | Inhibiting rate (%) |
| Vit C (control group) | 98.2±1.0 |
| Anthocyanidin | 58.6±2.2 |
The Chinese rose anthocyanidin of embodiment 7 suppresses the Activity Assessment that melanocyte (B16) generates melanin
By B16 cells, with 1 × 105After the density of/mL is inoculated in 96 orifice plates culture 24 hours, by extract and control
Group vitamin C is mixed in containing 100nM α-MSH without phenol red (phenol red free) nutrient solution, after reacting 72 hours, is taken
The μ L of supernatant 100 determine light absorption value (BioTek, SynergyTM2, USA) in 96 orifice plates in 405nm wavelength.Calculate extract
With vitamin C light absorption value in itself, conversion extract and vitamin C act on suppression Melanin productions ability after cell.
Result shows that Chinese rose anthocyanidin under the action time of 72 hours, has inhibition, in height under 100ppm concentration
The dosage 500ppm of concentration can have found have functions that significantly to suppress B16 Hemapoiesis melanin.
Embodiment August season anthocyanidin skin irritation test (patch test)
Assessment result：Chinese rose anthocyanidin skin irritation test result is displayed in observation in 24,48,72 hours and is pierced without skin
Swash property reaction.
Experimental technique：According to the regulation of International Contact Dermatitis seminar (ICDRG), observed in the way of vision,
The aqueous solution by obtained sample configuration into 10%, then 0.1ml is taken with pipette be placed in the plastics that 1.0 × 1.0cm encloses gauze
Disk, separately takes 0.1ml and filters secondary water as blank control group, is attached to Medial upper arm, is fixed with breathable adhesive tape, it is to avoid have a bath and do
It is wet, every gauze for more renewing for 24 hours, and the tested materials of 0.1ml and blank group are added again, observe 72 hours.Observation skin has
Without IRs such as redness, edema, papule, bubbles.(experiment person-time is 10 people).
Claims (6)
1. it is a kind of have whitening capability China rose anthocyanidin preparation method, it is characterised in that：Distill fresh with steam distillation
China rose, water is 4.5-5.5 with the ratio of flower:1, the water vapour after distillation obtains coolant, when the cooling for distilling out through cooling
Liquid weight is when adding 3-4 times of Chinese rose petal weight, that is, to stop distillation；After steam distillation, distiller bottom China rose
Liquid Residue boils filtering in 0.5-1.5 hours jointly with China rose in distiller, and filtrate removes pole high by DIAIONHP-20 tubing strings
Property composition, be concentrated in vacuo and drains after collecting efflux, then it is freeze-dried obtain final product Chinese rose anthocyanidin, put -20 DEG C of ice storage Tibetan.
2. it is a kind of have whitening capability China rose anthocyanidin preparation method, it is characterised in that：The coolant for distilling out is Chinese rose essence
Dew, containing natural Chinese rose fragrance, uses as natural Chinese rose water.
3. the purposes of China rose anthocyanidin, it is used to suppress DPPH free radicals, wherein described China rose anthocyanidin uses right
It is required that 1 method is prepared.
4. the purposes of China rose anthocyanidin, it is used to suppress tyrasamine enzymatic activity, wherein, described China rose anthocyanidin is used
The method of claim 1 is prepared.
5. the purposes of China rose anthocyanidin, melanin is generated for suppressing melanocyte, wherein, described China rose anthocyanidin
It is prepared using the method for claim 1.
6. the purposes of China rose anthocyanidin as claimed in claim 5, it is characterised in that when using, China rose anthocyanidin makes
It is 100-500ppm with concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611026841.5A CN106726868B (en) | 2016-11-22 | 2016-11-22 | A kind of preparation method for the China rose anthocyanidin having whitening capability |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611026841.5A CN106726868B (en) | 2016-11-22 | 2016-11-22 | A kind of preparation method for the China rose anthocyanidin having whitening capability |
Publications (2)
| Publication Number | Publication Date |
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| CN106726868A true CN106726868A (en) | 2017-05-31 |
| CN106726868B CN106726868B (en) | 2019-10-29 |
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| CN201611026841.5A Active CN106726868B (en) | 2016-11-22 | 2016-11-22 | A kind of preparation method for the China rose anthocyanidin having whitening capability |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1511583A (en) * | 2002-12-28 | 2004-07-14 | 石家庄制药集团制药技术开发有限公司 | Chinese rose extract and its preparing method and use |
| CN101311225A (en) * | 2007-05-26 | 2008-11-26 | 王晓明 | Process for abstracting kohlrabi pigment |
| CN102048662A (en) * | 2009-10-30 | 2011-05-11 | 爱普香料集团股份有限公司 | Method for manufacturing rose water containing natural rose pigment |
| CN102838884A (en) * | 2012-01-18 | 2012-12-26 | 天津天康源生物技术有限公司 | Prevention method of anthocyanin pigment crystal precipitate |
| CN103044953A (en) * | 2011-10-13 | 2013-04-17 | 黎嘉纯 | Extraction process for Chinese rose pigment |
| CN105106047A (en) * | 2015-08-07 | 2015-12-02 | 刘学勤 | Water-replenishing and skin-tendering mask |
| CN105693678A (en) * | 2016-01-15 | 2016-06-22 | 甘肃寿鹿山药业有限公司 | Method for extracting procyanidine from grape seeds |
-
2016
- 2016-11-22 CN CN201611026841.5A patent/CN106726868B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1511583A (en) * | 2002-12-28 | 2004-07-14 | 石家庄制药集团制药技术开发有限公司 | Chinese rose extract and its preparing method and use |
| CN101311225A (en) * | 2007-05-26 | 2008-11-26 | 王晓明 | Process for abstracting kohlrabi pigment |
| CN102048662A (en) * | 2009-10-30 | 2011-05-11 | 爱普香料集团股份有限公司 | Method for manufacturing rose water containing natural rose pigment |
| CN103044953A (en) * | 2011-10-13 | 2013-04-17 | 黎嘉纯 | Extraction process for Chinese rose pigment |
| CN102838884A (en) * | 2012-01-18 | 2012-12-26 | 天津天康源生物技术有限公司 | Prevention method of anthocyanin pigment crystal precipitate |
| CN105106047A (en) * | 2015-08-07 | 2015-12-02 | 刘学勤 | Water-replenishing and skin-tendering mask |
| CN105693678A (en) * | 2016-01-15 | 2016-06-22 | 甘肃寿鹿山药业有限公司 | Method for extracting procyanidine from grape seeds |
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| Publication number | Publication date |
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| CN106726868B (en) | 2019-10-29 |
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