CN106701600A - Straw fermenting agent, and preparation method and application thereof - Google Patents
Straw fermenting agent, and preparation method and application thereof Download PDFInfo
- Publication number
- CN106701600A CN106701600A CN201710063365.2A CN201710063365A CN106701600A CN 106701600 A CN106701600 A CN 106701600A CN 201710063365 A CN201710063365 A CN 201710063365A CN 106701600 A CN106701600 A CN 106701600A
- Authority
- CN
- China
- Prior art keywords
- ferment agent
- stalk
- stover ferment
- straw
- organic fertilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a straw fermenting agent. The straw fermenting agent is prepared from the following components in parts by weight: 0.2 to 0.4 part of aspergillus, 0.3 to 0.4 part of trichoderma, 0.1 to 0.2 part of coprinus sterquilinus, 0..05 to 0.15 part of white-rot fungi, 0.1 to 0.2 part of lactobacillus, 0.05 to 0.1 part of aspergillus niger and 0.05 to 0.1 part of bacillus subtilis. The invention also discloses a preparation method of the straw fermenting agent and application of the straw fermenting agent in preparing organic fertilizer. Microbial flora is used for fermenting straws to convert the straws into organic fertilizer, so that the straws can be effectively utilized, waste can be converted into treasure, not only is the problem of difficult straw treatment solved and the environmental pollution reduced, but also the high-quality organic fertilizer can be obtained by farmers; and moreover, the fermenting agent disclosed by the invention is short in fermenting time, the organic fertilizer obtained by fermenting the straws is high in content of organic matters and total nutrients, and the organic fertilizer can effectively improve soil, promote the growth of crops and remarkably increase the yield.
Description
Technical field
The invention belongs to fermentation technical field, and in particular to a kind of stover ferment agent and its preparation method and application.
Background technology
China produces about 700,000,000 tons of crop material per year, due to the transformation of country labor force transfer and tillage method, most of stalk
By on-site incineration or discarded.But, by stalk on-site incineration or discarded brought environmental pollution and problem of resource waste
Through as regulatory authorities and scientific research personnel's focus of attention.In stalk more than 60% be cellulose and hemicellulose, right and wrong
The biomass resource of Chang Youliang, by can be widely applied to the aspects such as feed, organic fertilizer, biogas after bioconversion.But, straw
The natural structure of stalk is extremely complex, and biotransformation efficiency is relatively low, and the difficulty for being directly used as the exploitation of above Related product is larger,
It is relatively costly.Therefore, it is a link mostly important during stalk resource is utilized stalk to be effectively treated, and is often adopted at present
With processing method be that stalk surrounding matrix is destroyed, such as:Composite construction depolymerization to hemicellulose and lignin, to increase
The enzyme-added contact with cellulose, and inhibition of the natural structure to substance transportation is reduced, while reducing the crystallization journey of cellulose
Degree.
The processing method of current stalk has physical treatment process, method of chemical treatment and biological treatment.
(1) physical treatment process:Wooden fibre can be to some extent destroyed using physical means such as high temperature, ultrasonic wave, microwaves
The natural structure of dimension, changes its stability.Stalk can realize the initial gross separation of each chemical constituent after steam blasting, and fiber becomes
Fluffy, specific surface area increase, hydrogen bond is reduced, structural rearrangement, is conducive to improving the accessibility of reagent, intensified response performance.But thing
Reason facture needs special equipment, and relatively costly, processing procedure has secondary pollution.
(2) method of chemical treatment:NaOH、H2O2, after the chemistry examination such as ammoniacal liquor processed stalk material, the fiber of stalk
There is a certain degree of destruction composition, form, crystal region, and stalk surface becomes loose, occur in that partial pore, the biology of stalk
Degradation property is improved.But chemical treatment can produce very serious secondary pollution.
(3) biological treatment:Fermentation process, stalk siliceous warty in appearance are carried out to stalk using efficient degradation bacteria
Structure comes off, and breakage on a large scale occurs in cell membrane, and whole stalk tissue becomes loose.Straw degradative bacterium can decompose each during it is organized
Polymer is planted, the lignin in stalk, cellulose and hemicellulose are decomposed into monomer or oligomer, improve its usability
Energy.Biochemical method cost is relatively low, does not have secondary pollution, but microorganism used therefor transformation efficiency in the biological treatment commonly used at present
Relatively low, process time is more long, and the content of organic matter for obtaining is low, have impact on the application and popularization of biological treatment.
The Chinese patent application of Application No. 201010509055.7 discloses a kind of direct decomposing and fermenting crops straws
The special microorganism composite bacterial agent for producing biogas and organic fertilizer is prepared and its application process.This special microorganism composite bacterial agent includes excrement
Terrible umbrella, mould, trichoderma, ink-cap (Coprinus atramentartius), after fermented culture, combination compounding is formed.But the composite bacteria fermentation stalk is produced
The content of organic matter that organic fertilizer is obtained is low and fermentation time is long.
The content of the invention
In view of this, it is an object of the invention to provide a kind of efficient stover ferment agent and its preparation method and application.
To achieve the above object, a kind of each component of stover ferment agent of present invention offer, including following parts by weight:
Including Aspergillus 0.2-0.4 parts, Trichoderma 0.3-0.4 parts, excrement ghost 0.1-0.2 parts of umbrella, cellulase 0.05-0.1 parts, white rot it is true
Bacterium 0.05-0.15 parts, Bacillus acidi lactici 0.1-0.2 parts, black-koji mould 0.05-0.1 parts, bacillus subtilis 0.05-0.1 parts.
Further, the stover ferment agent includes each component of following parts by weight:0.3 part of Aspergillus, Trichoderma
0.35 part, excrement ghost 0.1 part of umbrella, 0.05 part of cellulase, 0.15 part of white-rot fungi, 0.1 part of Bacillus acidi lactici, 0.1 part of black-koji mould,
0.1 part of bacillus subtilis.
Further, the stover ferment agent includes each component of following parts by weight:0.4 part of Aspergillus, Trichoderma 0.3
Part, 0.2 part of umbrella of excrement ghost, 0.1 part of cellulase, 0.05 part of white-rot fungi, 0.2 part of Bacillus acidi lactici, 0.05 part of black-koji mould, withered grass
0.075 part of bacillus.
Further, the stover ferment agent includes each component of following parts by weight:0.2 part of Aspergillus, Trichoderma 0.4
Part, excrement ghost 0.15 part of umbrella, 0.075 part of cellulase, 0.1 part of white-rot fungi, 0.15 part of Bacillus acidi lactici, 0.075 part of black-koji mould,
0.05 part of bacillus subtilis.
The present invention also provides a kind of preparation method of above-mentioned stover ferment agent, comprises the following steps:
Each component is weighed respectively according to formula rate, in input mixer, is well mixed, obtain the stover ferment agent.
Further, the rotating speed of the mixer is 300rpm~1000rpm, and mixing time is 5~20 minutes.
The present invention further provides a kind of application of above-mentioned stover ferment agent in for preparing organic fertilizer, including following step
Suddenly:
1) stalk is crushed to 2-4cm in stalk crasher, and with splicing bucket splicing known to volume measuring crushing
The cumulative volume of stalk;
2) will stover ferment agent add nutrient solution in activate, add water tune pH be 6.8-7.5 after it is standby;
3) by step 1) crushing straw that obtains loads mixing machine, and according to 1kg/m3-2kg/m3Ratio to the crushing straw
Stalk surface sprinkling step 2) activation after stover ferment agent, open batch mixing auger the crushing straw is stirred, by stirring
Uniform crushing straw is mixed to be banked up by splicing bucket splicing and in dry ground tapered, the temperature of above-mentioned crushing straw after 20 hours
When degree reaches 20 DEG C -30 DEG C, start fermentation;
4) by after the fermentation of 5-7 days, the crushing straw hand gently rubs i.e. broken, surface with the hands and fermentation knot during white hypha occurs
Beam, obtains organic fertilizer.
Further, step 1) stalk is to reject debris therein and the dry straw of rotten person of going rotten, or blue or green stalk;
It is highly preferred that the stalk is selected from one or more in wheat stalk, maize straw, broomcorn straw or soybean stalk.
Further, step 2) mass volume ratio of the stover ferment agent and nutrient solution is 1:(550-750), the straw
The temperature that stalk leavening is activated in nutrient solution is 30-32 DEG C, and the nutrient solution is the aqueous solution added with 2wt% white sugar.
Further, step 3) stover ferment agent fountain height be 1kg/m3。
Above-mentioned organic fertilizer can be as crops base fertilizer, it is also possible to which institute is applied in ditch spread or throwing in plantation has the soil of crops
State organic fertilizer, the organic fertilizer is using preceding needing to prevent solar exposure;Meanwhile, before fertilising, should not add on the soil
Adding of germicide, prevents the organic fertilizer from being contacted with bactericide, reduces fertilizer efficiency.
Compared with prior art, the present invention at least has advantages below:Microbial bacteria is used for the present invention fermentation of stalk,
Stalk is become organic fertilizer, stalk can be effectively utilized, stalk is turned waste into wealth, both solved stalk process problem, drop
Low environmental pollution, makes peasant obtain the organic fertilizer of high-quality again, and the ferment-fermented time of the invention is short, stalk fermentation is obtained
The organic matter and total nutrient content of the organic fertilizer for arriving are high, organic fertilizer can effective improved soil, promote plant growth, significantly improve product
Amount.
Specific embodiment
With reference to specific embodiment, the invention will be further described, and following examples are descriptive, is not limit
Qualitatively, it is impossible to which protection scope of the present invention is limited with this.
A kind of stover ferment agent, including following parts by weight each component:Including Aspergillus 0.2-0.4 parts, Trichoderma
0.3-0.4 parts, excrement ghost 0.1-0.2 parts of umbrella, cellulase 0.05-0.1 parts, white-rot fungi 0.05-0.15 parts, Bacillus acidi lactici 0.1-
0.2 part, black-koji mould 0.05-0.1 parts, bacillus subtilis 0.05-0.1 parts.
These microorganisms or enzyme can directly be bought or voluntarily cultivate.It is those skilled in the art to cultivate these microorganisms
Common skill.Following microorganisms are purchased in market, are briefly discussed below:
Aspergillus (are purchased from Shandong TianXing bio tech ltd, Latin literary fame:Aspergillus niger), it is common
Be aspergillus flavus, Fungi Imperfecti, a kind of common saprophytic fungus of aspergillus flavus group.Be more common in mouldy grain, grain product and other
On mould rotten organic matter.Colony growth is very fast, and short texture, surface celadon, the back side is colourless or slightly brownish.Thalline has many
Complicated branch mycelia is constituted.
Trichoderma (is purchased from Qingdao He Run Feng Nong fertilizer Co., Ltd, Latin literary fame:Trichoderma koningii), category
Eumycota, Deuteromycotina, Hyphomycetes, hyphomycetales, Moniliaceae, trichoderma is widely present in the soil under the conditions of varying environment
In earth.
Excrement ghost umbrella (is purchased from Shenzhen Yue Yun commerce and trade Co., Ltd, Latin literary fame:Coprinus sterqulinus), Agaricales
Coprinaceae Coprinus fungi plant.Fructification is medium.Bacteria cover diameter 2.5-4cm, 5-7cm high, initial stage short cylindrical shape or ellipse,
Pure white, there is scale, after be changed into cone, gradually open and flat, grey, middle part is light brown, and there is an obvious rib at edge, and taupe is to black
Color.Bacterial context white, it is relatively thin.Lamella white, the rear pink self-dissolving to black is black sap-shape.
White-rot fungi (is purchased from Nanjing Bai Lan Science and Technology Ltd.s, Latin literary fame:Phanerochaete
Chrysosporium Burdsall), it is that a class makes the white rotten fungi of timber, extracellular oxidizing ferment degraded wood can be secreted
Quality, and lignin degrading ability of the ability better than degraded cellulose, these enzymes can promote wooden rotting as light color
Spongy agglomerate-white rot, therefore referred to as white-rot fungi.
Bacillus acidi lactici (is purchased from Shandong sunflower bio tech ltd, Latin literary fame:Lacticacid
Bacteria), refer to can make carbohydrate fermentation produce lactic acid bacterium, have this bacterium in yoghurt;It is that a group lives in benefit in body
In the microorganism of host health, it safeguards that the effect of health and regulation immunologic function is widely recognized as.
Black-koji mould (is purchased from Shandong Changtai bio tech ltd, Latin literary fame:Aspergillus niger), half
Know bacterium subphylum, Hyphomycetes, hyphomycetales, Moniliaceae, a Common Species in aspergillus fungi;It is distributed widely in all over the world
In grain, plant product and soil;In fermentation industry mainly for the production of amylase, acid protease, cellulase, pectin
Enzyme, glucose oxidase, citric acid, gluconic acid and gallic acid etc..
Bacillus subtilis (is purchased from Shandong TianXing bio tech ltd, Latin literary fame:Bacillus
Subtilis), it is one kind of bacillus;0.7~0.8 × 2~3 microns of individual cells, uniform coloring;Without pod membrane, Zhousheng
Flagellum, can move;Gram-positive bacteria, 0.6~0.9 × 1.0~1.5 microns of gemma, ellipse to column, positioned at thalline center or
Slightly partially, thalline does not expand after sporulation.Bacterium colony rough surface is opaque, dirty white or slightly yellow, raw in liquid medium within
When long, wrinkle mould is commonly formed.
Cellulase (β-Isosorbide-5-Nitrae-glucan -4- glucan hydrolases, purchased from Shandong Su Kehan Science and Technology Ltd.s, Latin
Literary fame:Cellulase), it is the general name of the glucogenic one group of enzyme of degraded cellulose, it is not monomeric enzyme, but plays collaboration
The multicomponent enzyme system of effect, is a kind of complex enzyme, mainly by circumscribed 1,4 beta-glucanase, Endo-β-glucanase and beta-glucosidase
Enzyme etc. is constituted, the zytase of vigor also very high;Act on cellulose and the product being derived from cellulose.Microorganism
Cellulase destroys cell membrane into glucose and in Juice so as to improve fruit juice yield etc. in conversion insoluble fibrin
Aspect has very important significance.
The preparation method of above-mentioned stover ferment agent, comprises the following steps:Each component is weighed respectively according to formula rate, is put into
In mixer, in being stirred 5~20 minutes under 300rpm~1000rpm, it is well mixed, obtains the stover ferment agent.
Application of the above-mentioned stover ferment agent in for preparing organic fertilizer, comprises the following steps:
1) stalk is crushed to 2-4cm in stalk crasher, and with splicing bucket splicing known to volume measuring crushing
The cumulative volume of stalk;
2) will stover ferment agent add nutrient solution in activate, add water tune pH be 6.8-7.5 after it is standby;
3) by step 1) crushing straw that obtains loads mixing machine, and according to 1kg/m3-2kg/m3Ratio to the crushing straw
Stalk surface sprinkling step 2) activation after stover ferment agent, open batch mixing auger the crushing straw is stirred, by stirring
Uniform crushing straw is mixed to be banked up by splicing bucket splicing and in dry ground tapered, the temperature of above-mentioned crushing straw after 20 hours
When degree reaches 20 DEG C -30 DEG C, start fermentation;
4) by after the fermentation of 5-7 days, the crushing straw hand gently rubs i.e. broken, surface with the hands and fermentation knot during white hypha occurs
Beam, obtains organic fertilizer.
During specific implementation, step 1) stalk is to reject debris therein and the dry straw of rotten person of going rotten, or blue or green straw
Stalk;It is highly preferred that the stalk is selected from one or more in wheat stalk, maize straw, broomcorn straw or soybean stalk.
During specific implementation, step 2) stover ferment agent and the mass volume ratio of nutrient solution be 1:(550-750), it is described
The temperature that stover ferment agent is activated in nutrient solution is 30-32 DEG C, and the nutrient solution is the aqueous solution added with 2wt% white sugar.
During specific implementation, step 3) fountain height of the stover ferment agent is 1kg/m3。
Embodiment 1
The present embodiment provides a kind of stover ferment agent, including following parts by weight each component:0.3 part of Aspergillus, trichoderma
0.35 part of bacterium, 0.1 part of umbrella of excrement ghost, 0.05 part of cellulase, 0.15 part of white-rot fungi, 0.1 part of Bacillus acidi lactici, black-koji mould 0.1
Part, 0.1 part of bacillus subtilis.
The preparation method of above-mentioned stover ferment agent, comprises the following steps:Each component is weighed respectively according to formula rate, is put into
In mixer, in being stirred 15 minutes under 300rpm, it is well mixed, obtains the stover ferment agent.
Application of the above-mentioned stover ferment agent in for preparing organic fertilizer, comprises the following steps:
1) debris therein will be rejected and the wheat dry straw of rotten person of going rotten are crushed to 2-4cm in stalk crasher,
And with splicing bucket splicing known to volume measuring the cumulative volume of wheat crushing stalk;
2) will stover ferment agent add nutrient solution in activate, add water tune pH be 7.1 after it is standby;Wherein, the stalk fermentation
Agent is 1 with the mass volume ratio of nutrient solution:550, the temperature that the stover ferment agent is activated in nutrient solution is 30 DEG C, the battalion
Nutrient solution is the aqueous solution added with 2wt% white sugar;
3) by step 1) the wheat crushing stalk that obtains loads mixing machine, and according to 1.5kg/m3Ratio to the wheat flour
The surface sprinkling step 2 of crushing straw) stover ferment agent after activation, open batch mixing auger wheat crushing stalk stirring is equal
It is even, banked up by splicing bucket splicing and in dry ground by the wheat crushing stalk for stirring tapered, after 20 hours on
When the temperature for stating wheat crushing stalk reaches 20 DEG C, start fermentation;
4) by after the fermentation of 7 days, the wheat crushing stalk hand gently rubs i.e. broken, surface with the hands and fermentation knot during white hypha occurs
Beam, obtains the organic fertilizer, and its testing result is as shown in table 1.
Table 1
Embodiment 2
The present embodiment provides a kind of stover ferment agent, including following parts by weight each component:0.4 part of Aspergillus, trichoderma
0.3 part of bacterium, 0.2 part of umbrella of excrement ghost, 0.1 part of cellulase, 0.05 part of white-rot fungi, 0.2 part of Bacillus acidi lactici, black-koji mould 0.05
Part, 0.075 part of bacillus subtilis.
The preparation method of above-mentioned stover ferment agent, comprises the following steps:Each component is weighed respectively according to formula rate, is put into
In mixer, in being stirred 5 minutes under 1000rpm, it is well mixed, obtains the stover ferment agent.
Application of the above-mentioned stover ferment agent in for preparing organic fertilizer, comprises the following steps:
1) debris therein will be rejected and the corn dry straw of rotten person of going rotten are crushed to 2-4cm in stalk crasher,
And with splicing bucket splicing known to volume measuring the cumulative volume of crush maize stalk;
2) will stover ferment agent add nutrient solution in activate, add water tune pH be 7.5 after it is standby;Wherein, the stalk fermentation
Agent is 1 with the mass volume ratio of nutrient solution:750, the temperature that the stover ferment agent is activated in nutrient solution is 32 DEG C, the battalion
Nutrient solution is the aqueous solution added with 2wt% white sugar;
3) by step 1) the crush maize stalk that obtains loads mixing machine, and according to 2kg/m3Ratio to the crush maize
Stalk surface sprinkling step 2) activation after stover ferment agent, open batch mixing auger the crush maize stalk is stirred,
Banked up by splicing bucket splicing and in dry ground tapered, above-mentioned jade after 20 hours by the crush maize stalk for stirring
When the temperature of ground rice crushing straw reaches 30 DEG C, start fermentation;
4) by after the fermentation of 6 days, the crush maize stalk hand gently rubs i.e. broken, surface with the hands and fermentation knot during white hypha occurs
Beam, obtains the organic fertilizer, and its testing result is as shown in table 2.
Table 2
Embodiment 3
The present embodiment provides a kind of stover ferment agent, including following parts by weight each component:0.2 part of Aspergillus, trichoderma
0.4 part of bacterium, 0.15 part of umbrella of excrement ghost, 0.075 part of cellulase, 0.1 part of white-rot fungi, 0.15 part of Bacillus acidi lactici, black-koji mould
0.075 part, 0.05 part of bacillus subtilis.
The preparation method of above-mentioned stover ferment agent, comprises the following steps:Each component is weighed respectively according to formula rate, is put into
In mixer, in being stirred 12 minutes under 700rpm, it is well mixed, obtains the stover ferment agent.
Application of the above-mentioned stover ferment agent in for preparing organic fertilizer, comprises the following steps:
1) debris therein will be rejected and the sorghum dry straw of rotten person of going rotten are crushed to 2-4cm in stalk crasher,
And with splicing bucket splicing known to volume measuring the cumulative volume of sorghum crushing straw;
2) will stover ferment agent add nutrient solution in activate, add water tune pH be 6.8 after it is standby;Wherein, the stalk fermentation
Agent is 1 with the mass volume ratio of nutrient solution:650, the temperature that the stover ferment agent is activated in nutrient solution is 31 DEG C, the battalion
Nutrient solution is the aqueous solution added with 2wt% white sugar;
3) by step 1) crushing straw that obtains loads mixing machine, and according to 1kg/m3Ratio to the sorghum crushing straw
Surface sprinkling step 2) activation after stover ferment agent, open batch mixing auger the sorghum crushing straw is stirred, pass through
The sorghum crushing straw for stirring is banked up tapered, above-mentioned sorghum flour after 20 hours by splicing bucket splicing and in dry ground
When the temperature of crushing straw reaches 30 DEG C, start fermentation;
4) by after the fermentation of 5 days, the sorghum crushing straw hand gently rubs i.e. broken, surface with the hands and fermentation knot during white hypha occurs
Beam, obtains the organic fertilizer, and its testing result is as shown in table 3.
Table 3
Embodiment 4
The present embodiment provides a kind of stover ferment agent, including following parts by weight each component:0.3 part of Aspergillus, trichoderma
0.4 part of bacterium, 0.2 part of umbrella of excrement ghost, 0.06 part of cellulase, 0.15 part of white-rot fungi, 0.1 part of Bacillus acidi lactici, black-koji mould 0.1
Part, 0.1 part of bacillus subtilis.
The preparation method of above-mentioned stover ferment agent, comprises the following steps:Each component is weighed respectively according to formula rate, is put into
In mixer, in being stirred 12 minutes under 600rpm, it is well mixed, obtains the stover ferment agent.
Application of the above-mentioned stover ferment agent in for preparing organic fertilizer, comprises the following steps:
1) debris therein will be rejected and the wheat stalk of rotten person of going rotten, maize straw, broomcorn straw, soybean stalk is pressed
1:1:1:2-4cm is crushed in stalk crasher after 1 ratio mixing, and with splicing bucket splicing known to volume measuring powder
The cumulative volume of crushing straw mixture;
2) will stover ferment agent add nutrient solution in activate, add water tune pH be 7.0 after it is standby;Wherein, the stalk fermentation
Agent is 1 with the mass volume ratio of nutrient solution:700, the temperature that the stover ferment agent is activated in nutrient solution is 31 DEG C, the battalion
Nutrient solution is the aqueous solution added with 2wt% white sugar;
3) by step 1) crushing straw that obtains loads mixing machine, and according to 1kg/m3Table from ratio to the crushing straw
Face spray step 2) activation after stover ferment agent, open batch mixing auger the crushing straw mixture is stirred, by stirring
Uniform crushing straw mixture is mixed to be banked up by splicing bucket splicing and in dry ground tapered, above-mentioned crushing straw after 20 hours
When the temperature of stalk mixture reaches 30 DEG C, start fermentation;
4) by after the fermentation of 7 days, the crushing straw mixture hand gently rubs i.e. broken, surface with the hands and occurs being fermented during white hypha
Terminate, obtain organic fertilizer, its testing result is as shown in table 4.
Table 4
Embodiment 5
The correlated performance for not applying/applying the wheat of organic fertilizer described in embodiment 1-4 is compared below,
Its result is as shown in table 5.
Table 5
From table 5, first, the soil of the organic fertilizer being applied with described in embodiment 1-4 is not than applying the soil of organic fertilizer
It is more fertile.This is because, after adding the organic fertilizer described in embodiment 1-4, the organic fertilizer forms black or brown after becoming thoroughly decomposed
Organic colloid, i.e. humus.Humus is the organic matter of stabilization in soil, and soil fertility is played an important role, simultaneously because
The increase of the saprophytic animals and plants such as earthworm, effectively improves the situation of soil hardening, and it is loose porous that soil becomes, and is conducive to plant roots
The growth of system, meanwhile, the addition of the organic fertilizer has the effect of regulation soil acidity or alkalinity, reduces soil acidity, beneficial to plant
Growth.Secondly, the soil of organic fertilizer described in addition embodiment 1-4, the drought-resistant ability enhancing of the wheat of its plantation shows that addition should
The water holding capacity enhancing of the soil of organic fertilizer, the wheat of organic fertilizer described in addition embodiment 1-4 is compared to being not added with organic fertilizer
Wheat, its per mu yield substantially increases, and the per mu yield of organic fertilizer described in addition embodiment 3 reaches maximum 680kg/ mus, far above not adding
Plus 500kg/ mus of organic fertilizer, it is found that the height of per mu yield and organic fertilizer total nutrient and TOC in embodiment 1-4
Height it is closely related, with the increase of organic fertilizer total nutrient and TOC in embodiment 1-4, add corresponding organic fertilizer
Wheat per mu yield also correspondingly increase.Furthermore, the wheat of organic fertilizer described in addition embodiment 1-4 is compared to being not added with organic fertilizer
Wheat, its fruit growth cycle significantly reduces, and the fruit growth cycle for adding the organic fertilizer of embodiment 3 reaches minimum value 99 days,
Less than being not added with 120 days of organic fertilizer, it is found that the length in fruit growth cycle and organic fertilizer total nutrient in embodiment 1-4
And the height of TOC is closely related, with the increase of organic fertilizer total nutrient and TOC in embodiment 1-4,
The wheat fruit growth cycle for adding corresponding organic fertilizer also correspondingly reduces.Meanwhile, organic fertilizer is small described in addition embodiment 1-4
Wheat is compared to the wheat for being not added with embodiment 1-4 organic fertilizers, and Qi Mai Hui particles are bigger fuller.As can be seen here, with organic fertilizer
The growth of total nutrient and TOC, the growth cycle of wheat shortens and particle is fuller.In a word, it is small after soil improvement
Wheat can more fully absorb the nutrient and organic matter of machine fertilizer and effectively facilitate the growth of itself.
More than, the only present invention preferably specific embodiment, but the protection domain of invention is not limited thereto, any ripe
Know those skilled in the art the invention discloses technical scope in, the change or replacement that can be readily occurred in should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be defined by the protection domain of claims.
Claims (10)
1. a kind of each component of stover ferment agent, including following parts by weight:Including Aspergillus 0.2-0.4 parts, Trichoderma 0.3-
0.4 part, excrement ghost 0.1-0.2 parts of umbrella, cellulase 0.05-0.1 parts, white-rot fungi 0.05-0.15 parts, Bacillus acidi lactici 0.1-0.2
Part, black-koji mould 0.05-0.1 parts, bacillus subtilis 0.05-0.1 parts.
2. stover ferment agent according to claim 1, it is characterised in that the stover ferment agent includes following parts by weight
Each component:0.3 part of Aspergillus, 0.35 part of Trichoderma, excrement ghost 0.1 part of umbrella, 0.05 part of cellulase, 0.15 part of white-rot fungi,
0.1 part of Bacillus acidi lactici, 0.1 part of black-koji mould, 0.1 part of bacillus subtilis.
3. stover ferment agent according to claim 1, it is characterised in that the stover ferment agent includes following parts by weight
Each component:0.4 part of Aspergillus, 0.3 part of Trichoderma, 0.2 part of umbrella of excrement ghost, 0.1 part of cellulase, 0.05 part of white-rot fungi, breast
0.2 part of acidfast bacilli, 0.05 part of black-koji mould, 0.075 part of bacillus subtilis.
4. stover ferment agent according to claim 1, it is characterised in that the stover ferment agent includes following parts by weight
Each component:0.2 part of Aspergillus, 0.4 part of Trichoderma, excrement ghost 0.15 part of umbrella, 0.075 part of cellulase, 0.1 part of white-rot fungi,
0.15 part of Bacillus acidi lactici, 0.075 part of black-koji mould, 0.05 part of bacillus subtilis.
5. the preparation method of a kind of stover ferment agent according to claim any one of 1-4, it is characterised in that including as follows
Step:
Each component is weighed respectively according to formula rate, in input mixer, is well mixed, obtain the stover ferment agent.
6. preparation method according to claim 5, it is characterised in that the rotating speed of the mixer be 300rpm~
1000rpm, mixing time is 5~20 minutes.
7. application of a kind of stover ferment agent according to claim any one of 1-4 in for preparing organic fertilizer, it is special
Levy and be, comprise the following steps:
1) stalk is crushed to 2-4cm in stalk crasher, and with splicing bucket splicing known to volume measuring crushing straw
Cumulative volume;
2) will stover ferment agent add nutrient solution in activate, add water tune pH be 6.8-7.5 after it is standby;
3) by step 1) crushing straw that obtains loads mixing machine, and according to 1kg/m3-2kg/m3Ratio to the crushing straw
Spray step 2 in surface) stover ferment agent after activation, open batch mixing auger and the crushing straw stirs, it is equal by stirring
Even crushing straw is banked up tapered by splicing bucket splicing and in dry ground, and the temperature of above-mentioned crushing straw reaches after 20 hours
During to 20 DEG C -30 DEG C, start fermentation;
4) by after the fermentation of 5-7 days, the crushing straw hand gently rubs i.e. broken, surface with the hands and fermentation ends during white hypha occurs, obtains
To organic fertilizer.
8. application according to claim 7, it is characterised in that step 1) stalk is for rejecting debris therein and goes rotten
The dry straw of rotten person, or blue or green stalk;It is highly preferred that the stalk is selected from wheat stalk, maize straw, broomcorn straw or soybean
One or more in stalk.
9. application according to claim 7, it is characterised in that step 2) stover ferment agent and nutrient solution mass body
Product is than being 1:(550-750), the temperature that the stover ferment agent is activated in nutrient solution is 30-32 DEG C, and the nutrient solution is to add
There is the aqueous solution of 2wt% white sugar.
10. application according to claim 7, it is characterised in that step 3) stover ferment agent fountain height be 1kg/
m3。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710063365.2A CN106701600A (en) | 2017-02-03 | 2017-02-03 | Straw fermenting agent, and preparation method and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710063365.2A CN106701600A (en) | 2017-02-03 | 2017-02-03 | Straw fermenting agent, and preparation method and application thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN106701600A true CN106701600A (en) | 2017-05-24 |
Family
ID=58909800
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710063365.2A Pending CN106701600A (en) | 2017-02-03 | 2017-02-03 | Straw fermenting agent, and preparation method and application thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106701600A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107652128A (en) * | 2017-09-30 | 2018-02-02 | 安徽金帝豪肥业有限公司 | A kind of improving fertilizer based on crop material stalk and preparation method thereof |
CN109907158A (en) * | 2019-02-25 | 2019-06-21 | 车团结 | A kind of straw feed and its preparation and application |
CN115287216A (en) * | 2022-05-30 | 2022-11-04 | 内蒙古农业大学 | Straw degrading bacterium TXB2 and application thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102453676A (en) * | 2010-10-18 | 2012-05-16 | 嘉兴学院 | Straw starter and use of the straw starter in straw fermentation |
CN102690755A (en) * | 2012-06-01 | 2012-09-26 | 德州市元和农业科技开发有限责任公司 | Compound microbial bacterial preparation for degrading crop straw and preparation method and application of compound microbial bacterial preparation |
CN105368744A (en) * | 2015-11-30 | 2016-03-02 | 甘肃明德伟业生物科技有限公司 | Enzyme-bacterium duplex straw decomposing agent and its preparation and application methods |
-
2017
- 2017-02-03 CN CN201710063365.2A patent/CN106701600A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102453676A (en) * | 2010-10-18 | 2012-05-16 | 嘉兴学院 | Straw starter and use of the straw starter in straw fermentation |
CN102690755A (en) * | 2012-06-01 | 2012-09-26 | 德州市元和农业科技开发有限责任公司 | Compound microbial bacterial preparation for degrading crop straw and preparation method and application of compound microbial bacterial preparation |
CN105368744A (en) * | 2015-11-30 | 2016-03-02 | 甘肃明德伟业生物科技有限公司 | Enzyme-bacterium duplex straw decomposing agent and its preparation and application methods |
Non-Patent Citations (2)
Title |
---|
焦云鹏: "《酶制剂生产与应用》", 31 August 2015, 中国轻工业出版社 * |
顾碧华: "《新农村环境保护与治理》", 28 February 2010, 金盾出版社 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107652128A (en) * | 2017-09-30 | 2018-02-02 | 安徽金帝豪肥业有限公司 | A kind of improving fertilizer based on crop material stalk and preparation method thereof |
CN109907158A (en) * | 2019-02-25 | 2019-06-21 | 车团结 | A kind of straw feed and its preparation and application |
CN115287216A (en) * | 2022-05-30 | 2022-11-04 | 内蒙古农业大学 | Straw degrading bacterium TXB2 and application thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101225004B (en) | Method for producing soil biological fertilizer by employing compound starter | |
AU2020103489A4 (en) | A straw-decomposing bacterial agent and its application | |
CN109437989B (en) | Method for preparing culture medium by using pecan shells as raw materials | |
CN106631248A (en) | Production process for bioorganic fertilizer with high bacteria content | |
CN103833467A (en) | Organic compound fertilizer with citrus pomace and preparation method of organic compound fertilizer | |
CN100393672C (en) | Active additive for promoting agricultural waste material compost decomposition and its application | |
CN108315275A (en) | A kind of high yield " three plain enzymes " straw decomposing inoculant and preparation method thereof | |
CN104357362A (en) | Complex microbial agent for fast decaying straws to produce organic fertilizer as well as preparation method and application | |
CN106318884A (en) | Straw organic decomposition agent and preparation method thereof | |
CN110256145A (en) | A kind of tobacco organic fertilizer and preparation method thereof | |
CN103787708A (en) | Method for producing straw decomposition agent by utilizing filter mud, cassava residues and waste alcohol mash | |
CN110591970A (en) | Preparation method of straw-decomposing composite microbial inoculum | |
CN112047785A (en) | Chitin-containing environment-friendly enzyme biological fertilizer and preparation method thereof | |
CN106916021A (en) | A kind of double source humic acid biological fertilizer and preparation method and application | |
CN101205144A (en) | Technique for producing organic matrix by using herb residue as raw material and products produced thereby | |
CN106701600A (en) | Straw fermenting agent, and preparation method and application thereof | |
CN1326809C (en) | Refuse composting process adding different decay promoting ferments separately and its decay promoting ferments | |
CN108911859A (en) | A kind of preparation method of middle microelement microorganism fertilizer | |
CN104293701B (en) | A kind of composite bacteria agent rotten applied to High-efficiency straw speed and application | |
CN108410772A (en) | A kind of preparation method of straw biological decomposing agent | |
CN105585346A (en) | Active additive for promoting agricultural waste compost maturity and application thereof | |
CN108611293A (en) | Biological enzyme strain formula and preparation method in a kind of biological enzyme pulping process | |
CN106554224A (en) | A kind of high-efficiency fermenting type Hericium erinaceus (Bull. Ex Fr.) Pers. compost and preparation method thereof | |
CN113508660B (en) | Rape straw returning method | |
CN103787707A (en) | Method for producing straw decomposition agent by utilizing filter mud, cassava residues and waste alcohol mash |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170524 |