CN108611293A - Biological enzyme strain formula and preparation method in a kind of biological enzyme pulping process - Google Patents
Biological enzyme strain formula and preparation method in a kind of biological enzyme pulping process Download PDFInfo
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Abstract
The invention discloses biological enzyme strain formula in a kind of biological enzyme pulping process, it is to be grouped as by mushroom component and enzyme group, includes the percentage composition of following parts by weight：Bacillus, Bifidobacterium, lactic acid bacteria, saccharomycete, trichoderma reesei, aspergillus niger complex enzyme formulation, cellulase, laccase, protease, alpha-amylase, lignin degradation enzyme, phytase, dextranase, pectase, EDTA, vitamin C, glycine, cysteine, sodium chloride.The present invention discloses its preparation methods, the vigor of each component enzyme is high, and unit cost is low, and fermentation period is short, industrialization degree is high, it is simple for process, easy to use without increasing equipment applied to paper-making pulping, compared with traditional chemical pulp-making method, COD contents reduce by 35% or more in waste water, and production cost reduces by 22% or so, and improves the product quality of paper.
Description
Technical field
The invention belongs to biological enzyme pulping technique field, more particularly to biological enzyme strain is matched in a kind of biological enzyme pulping process
Side and preparation method.
Background technology
China is a large agricultural country, and biomass resource is extremely abundant, and crop stalk can be used to produce paper pulp, using each
Kind of biological enzyme is at moderate temperatures to the cellulose in straw, lignin, and hemicellulose is handled, degradation and softening fibre element,
Hemicellulose and pectin, carbohydrate is rapidly converted into other solvable and volatizable material because biological enzyme to it is wooden be known as it is soft
Change acts on, and degradable certain lignin alleviates the burden of water process under the premise of not influencing copy paper.
But traditional pulping process such as alkaline process, Ammonium Sulfite Method, sub- sodium method etc., in pulping process other than largely consuming energy,
It also will produce a large amount of black liquor in pulping digestion process, therefore expensive water treatment facilities need to be put into, increase papermaking cost,
It extends and builds payoff period.With the development of biological enzyme pulping technique field compound bio zymetology and microecology and increasingly complete
Kind, application of the biological enzyme slurrying additive in pulping and paper-making industry is more and more extensive.Compound biological enzyme pulping and paper-making additive
It is a kind of using the active compound biological enzyme of environmental beneficial and its preparation of metabolite, there is nonhazardous, noresidue and without dirt
The characteristics of dye, is of great significance to environmental protection.Currently, biological enzyme strain formula is ground in biological enzyme pulping process both at home and abroad
Study carefully exploitation and focuses primarily upon three classes：One kind is contained using bacillus subtilis and yeast culture as the active bacteria formulation of representative
The active ingredients such as the active material of compound biological enzyme growth and viable bacteria, can be effectively improved lignocellulosic degradation efficiency；One kind is
Using protease, amylase, lipase and cellulase as the enzyme preparation of representative, using the compound biological enzyme of high enzymatic productivity as
Strain is cultivated, enzyme material is obtained by fermentation process, enzyme preparation can directly be acted in plant fiber, promote the degradation of fiber
With abjection lignin；One kind be viable bacteria and enzyme compound biological enzyme pulping process in biological enzyme strain formula, with Tiny ecosystem tune
Control and pulping and paper-making utilize a variety of effects of promotion.
Invention content
Biological enzyme strain formula and preparation technique in a kind of biological enzyme pulping process of present invention offer, can improve beneficial bacterium
Competitiveness in fiber pulping process, the activity of active balance flora and biological enzyme promote to plant fiber pulping and paper-making
Ability to function, be applied to paper-making pulping, paper production cost and environmental pollution can be reduced, at the same help improve paper product product
Matter.
Biological enzyme strain formula in a kind of biological enzyme pulping process, be grouped as by mushroom component and enzyme group, including with
The percentage of lower parts by weight forms：Bacillus 1% 5.5%, Bifidobacterium 3% 10%, lactic acid bacteria 5% 15%, saccharomycete
5% 8%, trichoderma reesei 6% 8%, aspergillus niger complex enzyme formulation 10% 16%, cellulase 3% 6%, laccase 6% 8%, albumen
Enzyme 4% 7%, alpha-amylase 5% 8%, lignin degradation enzyme 2% 5%, phytase 6%-12%, dextranase 6%-12%, pectase
3% 5%, EDTA0.1%-0.2%, vitamin C 0.1%-0.5%, glycine 0.01%-0.1%, cysteine 0.2%-0.3%, chlorination
Sodium 1%-1.5%, the viable count content of microbial inoculum >=8*109cfu/g, cellulase 1000U/ ml, laccase 900u/ml~
1200u/ml, proteinase 8 0000U/ ml, alpha-amylase 40000U/ ml, lignin degradation enzyme 1000U/ ml, phytase
1000u/ml~1500u/ml, dextranase 5000U/ ml, pectase 800u/ml~1200u/ml.
Preferably, biological enzyme strain formula in a kind of biological enzyme pulping process, is grouped as by mushroom component and enzyme group,
Percentage composition including following parts by weight：Bacillus 3.5%, Bifidobacterium 8%, lactic acid bacteria 10%, saccharomycete 6%, Richter scale
Trichoderma 7%, aspergillus niger complex enzyme formulation 12%, cellulase 5%, laccase 6.5%, protease 6%, alpha-amylase 8%, lignin degradation
Enzyme 4.95%, phytase 7%, dextranase 9%, pectase 5%, EDTA0.2%, vitamin C 0.3%, glycine 0.05%, half Guang ammonia
Acid 0.3%, sodium chloride 1.2%, the viable count content of microbial inoculum >=8*109cfu/g。
The beneficial bacterium type and functional enzyme type that the invention contains are more, and fermentation respectively prepares the active bacteria formulation of culture：
Using raw materials such as wheat bran, corn and beans cypresses as bacillus, Bifidobacterium, lactic acid bacteria, saccharomycete and photosynthetic bacteria
Complex enzyme formulation is made in enzymatic production culture medium, compatibility；Active bacteria formulation and complex enzyme formulation are made by proper ratio compounding double
Imitate additive product.
Solid fermentation culture：The solid fermentation culture medium after sterilizing is taken, in the fermentation plant by disinfecting, cooling
To 40 45 DEG C, the distilled water of the aspergillus niger liquid seeds after culture is diluted into mixing, is uniformly sprayed onto solid fermentation culture medium
On, fermentation medium is mixed thoroughly, is laid in fermentation bed, thickness is 35 centimetres, 28 32 DEG C of temperature, relative humidity 80
90%；Primary at interval of 12 hours tilting tables, suitably fill spray distilled water；It co-cultures 48 hours；Then fermentation material is air-dried, is obtained black
Aspergillus complex enzyme formulation.
The active bacteria formulation is to answer bacillus, Bifidobacterium, lactic acid bacteria, saccharomycete and trichoderma reesei and aspergillus niger
Synthase preparation is inoculated in respectively in wheat bran and beans cypress enzymatic saccharification liquid, the canned seed culture medium 5L of 10L automated seeds fermentation,
It by 5% inoculum concentration, 28 32 DEG C, cultivates 20 hours, zymotic fluid passes through 40 50 DEG C of low temperature drying respectively.
The mass ratio of wheat bran and water in the mixture of wheat bran and water used in ferment tank is 2:3, wheat bran has
The granularity of 2mm to 8mm；Or the quality of the wheat bran and water in the mixture of wheat bran and water that uses of ferment tank
Than being 1:2, wheat bran powder has the granularity of 4mm to 5mm.
It is oligomeric that 1% is added in bacillus, Bifidobacterium, lactic acid bacteria, saccharomycete and trichoderma reesei and fermentation of Aspergillus niger liquid
Sugar passes through 40 50 DEG C of low temperature dryings, is made respectively as after protective agent.
The preparation of the enzyme preparation is by cellulase, laccase, protease, alpha-amylase, lignin degradation enzyme, phytic acid
The liquid seeds of enzyme, dextranase and pectase are sprayed on containing 60% wheat bran, 30% beans cypress and 0.4%KH2PO4Solid training
It supports in base, fermentation medium is mixed thoroughly, is laid in fermentation bed, thickness is about 35 centimetres, 28 32 DEG C of temperature, relative humidity
It is 80 90%；Primary at interval of 12 hours tilting tables, can suitably fill spray distilled water；It co-cultures 48 hours；Then by fermentation material wind
Dry, acquisition is answered by cellulase, laccase, protease, alpha-amylase, lignin degradation enzyme, phytase, dextranase and pectase
Enzyme preparation made of conjunction.
Active bacteria formulation is compounded according to the above ratio with complex enzyme formulation, while buffer solution is added, dual-purpose additive production is made
Product.
Paper making raw material is sent into digester and carries out enzymatic treatment after pretreatment, i.e., impregnates raw material with complex enzyme liquid.Due to adopting
With above-mentioned technical proposal, the present invention preferably realizes goal of the invention, and the vigor of each component enzyme is high, and unit cost is low, fermentation week
Phase is short, and industrialization degree is high, is applied to paper-making pulping, simple for process, easy to use without increasing equipment, with traditional length of schooling
Slurry processes compare, and COD contents reduce by 35% or more in waste water, and production cost reduces by 22% or so, and improves the product quality of paper.
(1) present invention contains beneficial bacterium, biological enzyme and functional materials oligosaccharide, the beneficial bacterium in formula and biological enzyme energy
The effect with living things catalysis is enough decomposed by viable bacteria, the pulping and paper-making utilization rate of fiber can be improved in a short time, promotes productivity
Can, after the product that the present invention is used in actual production, the utilization rate to thick pulping and paper-making can be improved.
(2) present invention does not contain anaerobic bacteria, does not need Anaerobic culturel process, so working condition is of less demanding, saves
Relevant equipment investment and production link, preparation process is simple, at low cost, is suitble to industrialization production.
(3) for the present invention using bacillus subtilis as active bacteria formulation strain, the culture propagation is very fast, can save viable bacteria system
Agent incubation time improves production efficiency；It further reduced as protective agent using oligosaccharide in active bacteria formulation drying process
Production cost can also significantly reduce the viable bacteria loss of active bacteria formulation preparation process, can meet the needs to pulping and paper-making, make
Pulping and paper-making additive, which enters effect in pulper, to be enhanced, and to can effectively improve peace weighing apparatus profitable strain, is dropped slurrying cost, is carried
High production performance.
Specific implementation mode
It elaborates below to the embodiment of the present invention, the present embodiment is carried out lower based on the technical solution of the present invention
Implement, gives detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
Biological enzyme strain formula in a kind of biological enzyme pulping process is grouped as by mushroom component and enzyme group, including following heavy
Measure the percentage composition of number：Bacillus 3.5%, Bifidobacterium 8%, lactic acid bacteria 10%, saccharomycete 6%, trichoderma reesei 7%, black song
Mould complex enzyme formulation 12%, laccase 6.5%, protease 6%, alpha-amylase 8%, lignin degradation enzyme 4.95%, is planted at cellulase 5%
Sour enzyme 7%, dextranase 9%, pectase 5%, EDTA0.2%, vitamin C 0.3%, glycine 0.05%, cysteine 0.3%, chlorination
Sodium 1.2%, the viable count content of microbial inoculum >=8*109cfu/g。
Preparation method：
Solid fermentation culture：The solid fermentation culture medium after sterilizing is taken, in the fermentation plant by disinfecting, is cooled to
40 45 DEG C, the distilled water of the aspergillus niger liquid seeds after culture is diluted into mixing, is uniformly sprayed onto on solid fermentation culture medium,
Fermentation medium is mixed thoroughly, is laid in fermentation bed, thickness is 4 centimetres, 30 DEG C of temperature, relative humidity 82%；It is small at interval of 12
When tilting table it is primary, suitably fill spray distilled water；It co-cultures 48 hours；Then fermentation material is air-dried, obtains aspergillus niger complex enzyme formulation.
The active bacteria formulation is to answer bacillus, Bifidobacterium, lactic acid bacteria, saccharomycete and trichoderma reesei and aspergillus niger
Synthase preparation is inoculated in respectively in wheat bran and beans cypress enzymatic saccharification liquid, the canned seed culture medium 5L of 10L automated seeds fermentation,
It by 5% inoculum concentration, 30 DEG C, cultivates 20 hours, zymotic fluid passes through 42 DEG C of low temperature drying respectively.
The mass ratio of wheat bran and water in the mixture of wheat bran and water used in ferment tank is 2:3, wheat bran has
The granularity of 6mm；
1% oligosaccharide work is added in bacillus, Bifidobacterium, lactic acid bacteria, saccharomycete and trichoderma reesei and fermentation of Aspergillus niger liquid
After protective agent, it is made respectively by 45 DEG C of low temperature dryings.
The preparation of the enzyme preparation is by cellulase, laccase, protease, alpha-amylase, lignin degradation enzyme and pectin
The liquid seeds of enzyme are sprayed on containing 60% wheat bran, 30% beans cypress and 0.4%KH2PO4Solid medium in, by fermented and cultured
Base is mixed thoroughly, is laid in fermentation bed, and thickness is about 4 centimetres, 30 DEG C of temperature, relative humidity 85%；At interval of 12 hours tilting tables one
Secondary, can suitably fill spray distilled water；It co-cultures 48 hours；Then fermentation material is air-dried, obtain by cellulase, laccase, protease,
The enzyme preparation that alpha-amylase, lignin degradation enzyme and pectase are combined.
Embodiment 2
Biological enzyme strain formula in a kind of biological enzyme pulping process is grouped as by mushroom component and enzyme group, including following heavy
Measure the percentage composition of number：Bacillus 5.5%, Bifidobacterium 10%, lactic acid bacteria 5%, saccharomycete 5%, trichoderma reesei 8%, black song
Mould complex enzyme formulation 13%, cellulase 6%, laccase 8%, protease 4%, alpha-amylase 5%, lignin degradation enzyme 2%, phytase
12%, dextranase 12%, pectase 3%, EDTA0.1, vitamin C 0.14%, glycine 0.01%, cysteine 0.25%, chlorination
Sodium 1%, the viable count content of microbial inoculum >=8*109cfu/g。
The preparation method is the same as that of Example 1.
Embodiment 3
Biological enzyme strain formula in a kind of biological enzyme pulping process is grouped as by mushroom component and enzyme group, including following heavy
Measure the percentage composition of number：Bacillus 4%, Bifidobacterium 6%, lactic acid bacteria 6%, saccharomycete 8%, trichoderma reesei 8%, aspergillus niger
Complex enzyme formulation 16%, cellulase 6%, laccase 8%, protease 7%, alpha-amylase 5%, lignin degradation enzyme 3.6%, phytase
10%, dextranase 7%, pectase 3%, EDTA0.1%, vitamin C 0.5%, glycine 0.1%, cysteine 0.2%, sodium chloride
1.5%, the viable count content of microbial inoculum >=8*109cfu/g。
The preparation method is the same as that of Example 1.
Embodiment 4
Biological enzyme strain formula in a kind of biological enzyme pulping process is grouped as by mushroom component and enzyme group, including following heavy
Measure the percentage composition of number：Bacillus 3.5%, Bifidobacterium 8%, lactic acid bacteria 10%, saccharomycete 6%, trichoderma reesei 7%, black song
Mould complex enzyme formulation 12%, cellulase 5%, laccase 6.5%, protease 6%, alpha-amylase 8%, lignin degradation enzyme 5%, phytase
9%, dextranase 9%, pectase 5%, the viable count content of microbial inoculum >=8*109cfu/g。
The preparation method is the same as that of Example 1.
Embodiment 5
Biological enzyme strain formula in a kind of biological enzyme pulping process includes the percentage composition of following parts by weight：Bacillus
9%, Bifidobacterium 17%, lactic acid bacteria 21%, saccharomycete 13%, trichoderma reesei 15%, aspergillus niger complex enzyme formulation 25%, the work of microbial inoculum
Bacterium number content >=8*109cfu/g.The method of strain during the preparation method is the same as that of Example 1.
Embodiment 6
Biological enzyme formula in a kind of biological enzyme pulping process includes the percentage composition of following parts by weight：Cellulase 10%,
Laccase 12%, protease 12%, alpha-amylase 14%, lignin degradation enzyme 10%, phytase 17%, dextranase 15%, pectase
10%.The method of biological enzyme during the preparation method is the same as that of Example 1.
Embodiment 7
Biological enzyme strain formula in a kind of biological enzyme pulping process includes the percentage composition of following parts by weight：Bacillus
19%, Bifidobacterium 27%, lactic acid bacteria 31%, saccharomycete 23%, the viable count content of microbial inoculum >=8*109cfu/g.Preparation method
With the method for the strain in embodiment 1.
Performance test：
Raw material chooses the fibrous plants such as wheat straw, straw, cornstalk, bagasse, reed, cotton stem, poplar, first adopts raw material
It is sent into chaffcutter and is shredded with belt conveyor, then cleaned through bipyramid deduster, then communicated band, pre- material conveyer are sent
Enter after presoaking in tank, the biological enzyme strain that the embodiment preparation of equivalent is added is handled.
Claims (6)
1. biological enzyme strain formula in a kind of biological enzyme pulping process, is to be grouped as by mushroom component and enzyme group, feature exists
In, include following parts by weight percentage composition：Bacillus 1% 5.5%, Bifidobacterium 3% 10%, lactic acid bacteria 5%
15%, saccharomycete 5% 8%, trichoderma reesei 6% 8%, aspergillus niger complex enzyme formulation 10% 16%, cellulase 3% 6%, laccase
6% 8%, protease 4% 7%, alpha-amylase 5% 8%, lignin degradation enzyme 2% 5%, phytase 6%-12%, dextranase
6%-12%, pectase 3% 5%, EDTA0.1%-0.2%, vitamin C 0.1%-0.5%, glycine 0.01%-0.1%, cysteine
0.2%-0.3%, sodium chloride 1%-1.5%, the viable count content of microbial inoculum >=8*109cfu/g, cellulase 1000U/ ml, paint
Enzyme 900u/ml~1200u/ml, proteinase 8 0000U/ ml, alpha-amylase 40000U/ ml, lignin degradation enzyme 1000U/
Ml, phytase 1000u/ml~1500u/ml, dextranase 5000U/ ml, pectase 800u/ml~1200u/ml.
2. biological enzyme strain formula in a kind of biological enzyme pulping process according to claim 1, which is characterized in that including with
The percentage of lower parts by weight forms：Bacillus 3.5%, Bifidobacterium 8%, lactic acid bacteria 10%, saccharomycete 6%, trichoderma reesei 7%,
Aspergillus niger complex enzyme formulation 12%, cellulase 5%, laccase 6.5%, protease 6%, alpha-amylase 8%, lignin degradation enzyme
4.95%, phytase 7%, dextranase 9%, pectase 5%, EDTA0.2%, vitamin C 0.3%, glycine 0.05%, cysteine
0.3%, sodium chloride 1.2%, the viable count content of microbial inoculum >=8*109cfu/g。
3. the preparation method of biological enzyme strain formula, feature in a kind of a kind of biological enzyme pulping process described in claim 1
It is, takes the solid fermentation culture medium after sterilizing, in the fermentation plant by disinfecting, is cooled to 40 45 DEG C, will train
Aspergillus niger liquid seeds after supporting dilute mixing with distilled water, are uniformly sprayed onto on solid fermentation culture medium, by fermentation medium
It mixes thoroughly, is laid in fermentation bed, thickness is 35 centimetres, 28 32 DEG C of temperature, and relative humidity is 80 90%；It is small at interval of 12
When tilting table it is primary, suitably fill spray distilled water；It co-cultures 48 hours；Then fermentation material is air-dried, obtains aspergillus niger complex enzyme formulation.
4. the preparation method of biological enzyme strain formula, feature in a kind of biological enzyme pulping process according to claim 3
It is, bacillus, Bifidobacterium, lactic acid bacteria, saccharomycete and trichoderma reesei and aspergillus niger complex enzyme formulation is inoculated with respectively
In wheat bran and beans cypress enzymatic saccharification liquid, 10L automated seeds ferment canned seed culture medium 5L, by 5% inoculum concentration, 28
It 32 DEG C, cultivates 20 hours, zymotic fluid passes through 40 50 DEG C of low temperature drying respectively.
5. the preparation method of biological enzyme strain formula, feature in a kind of biological enzyme pulping process according to claim 4
It is, the mass ratio of wheat bran and water in the mixture of wheat bran and water used in the ferment tank is 2:3, wheat bran tool
There is the granularity of 2mm-8mm；Or the matter of the wheat bran and water in the mixture of wheat bran and water that uses of ferment tank
Amount is than being 1:2, wheat bran powder has the granularity of 4mm-5mm.
6. the preparation method of biological enzyme strain formula, feature in a kind of a kind of biological enzyme pulping process described in claim 1
It is, by cellulase, the liquid of laccase, protease, alpha-amylase, lignin degradation enzyme, phytase, dextranase and pectase
Body seed is sprayed on containing 60% wheat bran, 30% beans cypress and 0.4%KH2PO4Solid medium in, fermentation medium is mixed
It is even, it is laid in fermentation bed, thickness is about 35 centimetres, 28 32 DEG C of temperature, and relative humidity is 80 90%；It is small at interval of 12
When tilting table it is primary, can suitably fill spray distilled water；It co-cultures 48 hours；Then fermentation material is air-dried, is obtained by cellulase, paint
The enzyme preparation that enzyme, protease, alpha-amylase, lignin degradation enzyme, phytase, dextranase and pectase are combined.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114837007A (en) * | 2022-06-01 | 2022-08-02 | 齐鲁工业大学 | Method for pulping wheat straw by using composite microbial inoculum |
| NL2033571A (en) * | 2022-11-18 | 2022-12-15 | Hong Kong Morning Star International Holding Ltd | Biological Pulping Compound Enzyme Formula and Its Preparation Method |
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| CN104499336A (en) * | 2014-11-30 | 2015-04-08 | 邵素英 | Bleaching compound enzyme for papermaking and preparation method of bleaching compound enzyme |
| CN105316366A (en) * | 2015-10-20 | 2016-02-10 | 陈国章 | Production method for comprehensively utilizing biological straw |
| CN106467903A (en) * | 2016-09-18 | 2017-03-01 | 齐鲁工业大学 | A kind of Bacillus licheniformis engineering bacteria being applied to pulping process and its construction method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103642774A (en) * | 2013-11-13 | 2014-03-19 | 宁夏夏盛实业集团有限公司 | Mixed neutral cellulase, preparation method thereof and application thereof to papermaking beating |
| CN104499336A (en) * | 2014-11-30 | 2015-04-08 | 邵素英 | Bleaching compound enzyme for papermaking and preparation method of bleaching compound enzyme |
| CN105316366A (en) * | 2015-10-20 | 2016-02-10 | 陈国章 | Production method for comprehensively utilizing biological straw |
| CN106467903A (en) * | 2016-09-18 | 2017-03-01 | 齐鲁工业大学 | A kind of Bacillus licheniformis engineering bacteria being applied to pulping process and its construction method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114837007A (en) * | 2022-06-01 | 2022-08-02 | 齐鲁工业大学 | Method for pulping wheat straw by using composite microbial inoculum |
| NL2033571A (en) * | 2022-11-18 | 2022-12-15 | Hong Kong Morning Star International Holding Ltd | Biological Pulping Compound Enzyme Formula and Its Preparation Method |
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