CN105585346A - Active additive for promoting agricultural waste compost maturity and application thereof - Google Patents
Active additive for promoting agricultural waste compost maturity and application thereof Download PDFInfo
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- CN105585346A CN105585346A CN201410637051.5A CN201410637051A CN105585346A CN 105585346 A CN105585346 A CN 105585346A CN 201410637051 A CN201410637051 A CN 201410637051A CN 105585346 A CN105585346 A CN 105585346A
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Abstract
The invention relates to enzyme liquid treatment on agricultural waste compost. An active additive for promoting agricultural waste compost maturity is a composite enzyme liquid which is produced by mixing and fermenting bacillus, aspergillus niger, trichoderma and penicillium according to certain proportion. The fermentation liquid, after being filtered, is directly added to the compost by 0.5-1% by weight of compost raw materials when the agricultural waste compost begins. The active additive contains abundant pectinase and lingocellulase and can promote degradation on pectin, hemicelluloses, cellulose and the like in a primary fermentation step and break on cell wall tissue of plants, so that the microorganisms can attack the cellulose and lignin more easily during a high-temperature step and a decay step, thereby accelerating the compost maturity process and improving the quality of the compost.
Description
Technical field
The present invention relates to Solid Waste Treatment, be specifically related to a kind of enzyme liquid place that promotes agricultural waste material compost decompositionReason.
Background technology:
China is large agricultural country, and the annual a large amount of agricultural wastes that produce not only affect environment, the especially wasting of resources.Aerobic compost is the important means of agricultural wastes resource and harmless treatment, due to agricultural wastes, especially doesIn thing stalk, be rich in the lignocellulosic of difficult degradation, make it have that the Composting treatment cycle is long, compost quality is low etc.Problem. Microbe inoculation thalline is the method for current conventional promotion compost process, at ZL03118137.6Patent of invention " twice microbe inoculation composite bacteria agent capable composting process " in, propose at hot stage and secondary fermentationTemperature-fall period add respectively 1# composite bacteria agent capable and 2# composite bacteria agent capable, bring into play their ligocellulose degradation's energyPower, the quick composting of promotion compost. But the method for inoculating at present thalline is all based on ligocellulose degradationMainly occur in hot stage and become thoroughly decomposed the stage, thereby being played a role by thermophilic actinomycete, middle temperature fungi respectively.Generally all think and do not degrade at compost one time fermentation initial stage lignocellulosic, take method to promote in this stageHardly degraded organic substance degrades to accelerate compost process there are no report.
Due to the cell membrane of plant tissue mainly by pectin, cellulose, hemicellulose and lignin mutually chimeric andBecome, pectin, hemicellulose, cellulosic degraded can significantly destroy cell wall structure, strengthen the infiltration of cell membraneProperty, therefore these materials can significantly improve later stage microorganism to lignin, cellulosic in effective degraded in early stageAttack. The one time fermentation stage of compost, owing to containing a large amount of easily biodegradable organicses, this in stage add pectin,Lignocellulose degrading bacteria can not obviously play the effect of this type of material of degraded. Therefore how at the beginning of compost fermentationPhase is impelled lignocellulose mass degradation, must contribute to accelerate becoming thoroughly decomposed of compost.
Summary of the invention:
The object of the invention is intended to promote the hardly degraded organic substance such as pectin, lignocellulosic at the beginning of the one time fermentation stagePhase degraded, thus Composting process accelerated, improve compost end product quality.
Foregoing invention object is achieved through the following technical solutions:
Promote an active additive for agricultural waste material compost decomposition, be mould by bacillus, aspergillus niger, wood,Prepared by mould mixed culture fermentation contain pectase, lignocellulolyticenzymes, zytase and polyphenol oxidase fermentsLiquid is complex enzyme liquid;
Preparing bacillus, aspergillus niger, the wood seed liquor mould, mould of complex enzyme liquid mixes with following volume ratioFermentation:
Promote the application of the active additive of agricultural waste material compost decomposition, by sending out through elimination thalline and solid matrixFerment liquid, added by 0.5～1% of composting material weight at the composting initial stage.
Be described in further detail the present invention below in conjunction with accompanying drawing.
Brief description of the drawings:
In Fig. 1 composting process, lignocellulose degradation rate changes;
In Fig. 2 composting process, percentage of seedgermination changes;
The preparation of active additive is through following two steps:
1, seed culture: bacillus, aspergillus niger, wood that purifying is cultivated are mould, mould is respectively from preservationOn slant medium, be seeded to beef-protein medium, Cha Shi culture medium, malt extract medium and potatoSM, fills culture medium 200ml in the fermentation flask of each 1L, 35～37 DEG C of cultivation temperature, and shaking table turnsSpeed 180～200rpm, cultivates 24～36h.
2, fermented and cultured: after above-mentioned seed culture fluid is mixed in following ratio, with 5～10% inoculum concentrationBe seeded in fermentation medium.
Seed culture fluid mixed proportion:
Fermented and cultured based component: beancake powder 2～4%, rice straw powder 1～2%, wheat bran 0.75～1%NH4NO30.5％，CaCl20.2%. The condition of culture of fermentation tank is 35～37 DEG C of temperature, mixing speed 450～550rpm,Throughput 1.1～1.3vvm. Cultivate after 3～5d, zymotic fluid is removed thalline and solid-based through four layers of filtered through gauzeAfter matter, obtain the compost active additive of multi-enzyme system.
The active additive that fermentation produces as stated above has higher pectase work and lignocellulolyticenzymes lives,Pectase work can reach 74～120Uml-1, xylanase activity can reach 60～100Uml-1, Filter paper CellulaseWork can reach 32～45Uml-1, polyphenol oxidase activity can reach 1～3.5Uml-1. Agricultural wastes are adjusted through pulverizingAfter joint C/N ratio, add active additive with 0.5～1% of composting material weight, fully mix rear upper heap.
In agricultural raw material, pectin and wood fibre cellulose content are higher, although these materials compared with difficult degradation, byHave certain water stain property, especially raw material after fragmentation in them, adding of active additive can effectively startThe degraded of compost initial stage pectin and lignocellulosic, makes plant tissue cell's wall generation disintegration, after contributing toThe further effect of phase lignocellulose degrading bacteria, improves lignocellulose degradation speed and degradation rate. There is activity to addThe heap body that adds agent all increased at compost initial stage and the various degradation effect of organic compound of later stage. Compost one time fermentationProcess pectin, hemicellulose, Fiber and lignin concentration respectively from 3.61%, 22.31%, 31.02%,14.38% is down to 3.19%, 20.24%, 29.05%, 14.13%, and its degradation rate be you can well imagine than contrast componentHigh by 4.99%, 4.08%, 3.16%, 0.97%; Compost secondary fermentation process pectin, hemicellulose,Fiber and lignin concentration is respectively 1.4%, 7.25%, 13.86%, 13.03%, its degradation rate comparisonYou can well imagine high 12.19%, 15.73%, 12.02%, 3.06% according to component. Meanwhile, percentage of seedgermination in advanceWithin 5 days, arrive 80%, compost maturity process is accelerated, and compost quality of finished improves.
Detailed description of the invention:
1, seed culture: by bacillus subtilis (Bacillussubtilis), aspergillus niger(Aspergillusniger), Trichoderma viride (Trichodermaviride), simple mould(Penicilliumsimplicissimum) respectively from slant medium be seeded to beef-protein medium,Cha Shi culture medium, malt extract medium and potato sucrose culture medium, fill culture medium 200ml in 1L triangular flask,37 DEG C, 200rpm, cultivate 30h.
2, fermented and cultured: fill fermentation medium 4L in 5L automatic fermenter, seed culture fluid is pressed respectively18%, 35%, 22%, 25% ratio is mixed, and then by 8%, seed mixture liquid is seeded to fermentation tank,Under 37 DEG C, 500rpm, 1.2vvm condition, cultivate 4d.
3, the zymotic fluid obtaining is active additive after removing thalline and solid matrix by four layers of filtered through gauze.After measured, this active additive pectase is lived as 78.4Uml-1, xylanase activity is 65.9Uml-1, filterPaper cellulose enzyme is lived as 42.3Uml-1, polyphenol oxidase activity is 2.4Uml-1。
4, gather Changsha suburb farming refuse, comprise all kinds of dish leaves, stalk etc., regulate with wheat bran after crushedC/N is 25, and moisture content is 55%, and organic matter is 70%, is 03118137.6 by number of patent application simultaneouslyPatent of invention " twice microbe inoculation composite bacteria agent capable composting process " add composite bacteria agent capable A (pseudomonas aeruginosaPsedomanasaeruginosa25%, Bacillus subtilis 20%, aspergillus oryzaeAspergillusoryzae25%, Candida lipolytica Candidalipolytica20%, brown ball is solidNitrogen bacterium Azotobacterchroococcum10%), active additive is added to raw material with 1% weight ratio,Mix rear upper storehouse formula composting device, the dischargeable capacity of device is 0.8m3. The temperature for the treatment of compost material rises toAfter 55～60 DEG C, add composite bacteria agent capable B (the mould Trichodermakoningii35% of healthy and free from worry wood, muck fiber listBorn of the same parents bacterium Cellulomonasfimi30%, Phanerochaete chrysosporiumPhanerochetechrysosporium35%). The raw material sources of control treatment are identical with pretreatment, do not addActive additive, other composting methods are identical.
In composting process, detect crude fiber content, and detect germination index, two kinds of differences with compost extractionThe reaction effect of composting process is shown in shown in Fig. 1 and Fig. 2.
By relatively finding to add active additive in compositing system, can accelerate coarse-fibred degraded, its degradedRate has improved 11.34%. In addition, active additive can improve germination index, and makes its 5 days in advanceReach 80%, think that active additive can accelerate compost maturity, improve composting efficiency and quality of finished. This is livedProperty additive produce and application process simple, combine with the composting process of other microbial inoculants simultaneously, have moreGood application prospect.
Claims (3)
1. promote an active additive for agricultural waste material compost decomposition, it is characterized in that this active additive be byBacillus, aspergillus niger, wood is mould, prepared by mould mixed culture fermentation contains pectase, lignocellulolyticenzymes, woodThe zymotic fluid of dextranase and polyphenol oxidase is complex enzyme liquid.
2. as a kind of active additive that promotes agricultural waste material compost decomposition of claim 1, its feature existsMix and send out with following volume ratio in bacillus, aspergillus niger, the wood of preparing complex enzyme liquid seed liquor mould, mouldFerment:
3. an application for the active additive of promotion agricultural waste material compost decomposition as claimed in claim 1, itsBe characterised in that the zymotic fluid through elimination thalline and solid matrix, the composting initial stage press 0.5 of composting material weight～1% adds.
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| CN201410637051.5A CN105585346A (en) | 2014-10-30 | 2014-10-30 | Active additive for promoting agricultural waste compost maturity and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106424129A (en) * | 2016-10-13 | 2017-02-22 | 湖南大学 | Method for remedying soil contaminated with 2,2',4,4'-tetrabromodiphenyl ether |
| CN108976076A (en) * | 2018-07-27 | 2018-12-11 | 田东县文设芒果专业合作社 | A kind of preparation method improving the active dedicated fertilizer of mango |
| CN113816780A (en) * | 2021-11-22 | 2021-12-21 | 山东绿达建设发展集团有限公司 | Composting treatment method for landscaping waste |
-
2014
- 2014-10-30 CN CN201410637051.5A patent/CN105585346A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106424129A (en) * | 2016-10-13 | 2017-02-22 | 湖南大学 | Method for remedying soil contaminated with 2,2',4,4'-tetrabromodiphenyl ether |
| CN106424129B (en) * | 2016-10-13 | 2019-12-06 | 湖南大学 | Method for repairing 2,2 ', 4, 4' -tetrabromobisphenol contaminated soil |
| CN108976076A (en) * | 2018-07-27 | 2018-12-11 | 田东县文设芒果专业合作社 | A kind of preparation method improving the active dedicated fertilizer of mango |
| CN113816780A (en) * | 2021-11-22 | 2021-12-21 | 山东绿达建设发展集团有限公司 | Composting treatment method for landscaping waste |
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Addressee: CHONGQING GUANTAI TECHNOLOGY CO., LTD. Document name: Notification of Publication of the Application for Invention |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160518 |
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