CN106588303A - Preparation method and application of crop straw organic fertilizer containing poly-Y-glutamicacid - Google Patents
Preparation method and application of crop straw organic fertilizer containing poly-Y-glutamicacid Download PDFInfo
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- CN106588303A CN106588303A CN201611088448.9A CN201611088448A CN106588303A CN 106588303 A CN106588303 A CN 106588303A CN 201611088448 A CN201611088448 A CN 201611088448A CN 106588303 A CN106588303 A CN 106588303A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a preparation method of a crop straw organic fertilizer containing poly-Y-glutamicacid. According to the method, crop straw is used as a base material, a microorganism strain is added, high-temperature compost fertilization is matched with the effective component poly-Y-glutamicacid, and accordingly the crop straw organic fertilizer containing the poly-Y-glutamicacid is prepared. The preparation technology is simple, low in cost and high in controllability. The crop straw organic fertilizer containing the poly-Y-glutamicacid is high in yield, free of environmental pollution and balanced in nutrient content; and the crop straw organic fertilizer containing the poly-Y-glutamicacid contains major elements of nitrogen, phosphorus and potassium, and rich microelements and beneficial microorganisms, and under the synergistic effect of the poly-Y-glutamicacid, a good effect on improving soil, raising soil fertility and improving crop quality can be achieved. The crop straw organic fertilizer containing the poly-Y-glutamicacid prepared through the method can be applied to soil fertilization of vegetable crops, grain crops and commercial crops.
Description
Technical field
The invention belongs to field of fertilizer production, and in particular to a kind of system of the agricultural crop straw fertilizer containing Y- polyglutamic acids
Preparation Method and application.
Background technology
The ecological agriculture is promoted, is preserved the ecological environment and agricultural land soil, just become environmental conservation and paying close attention to agro-technician
The focus of research.But, with the increase of fertilizer application amount, to also occurring in that a series of problem while increasing crop yield：
The dissolving of general chemistry fertilizer is too fast, heat is decomposed, and adds rainwash, subsurface wastewater infiltration system, unfavorable meteorological condition etc., fertilizer loss
Seriously, utilization rate of fertilizer is low, not only causes the energy, the waste of resource and the loss of economy, also creates to ecologies such as water sources
The severe contamination of environment, the life-time service of chemical fertilizer can be made to farmland ecosystem, soil physico-chemical property and soil microorganism system
Into serious destruction, cause soil compaction.
From increasing fertilizer efficiency, improve utilization rate of fertilizer, and soil quality and comprehensive fertility can be improved,
Do not remain in soil, fertilizer synergist cheap and easy to get, just becoming the focus that environmental conservation and agro-technician pay close attention to research.
Y- polyglutamic acids are the copolymerized macromolecule polymer with glutamic acid as only monomer, used as the biology system of pure natural
Agent, can natural decomposition generate aminoacid, do not result in the secondary pollution to environment.And, Y- polyglutamic acids are used as fertilizer synergistic
Agent, agriculturally has following effect：(1) Y- polyglutamic acids are dissolved in aqueous solution and easily form hydrogel, the fertilizer that wraps up it, micro-
Secondary element has obvious slow release effect, the fertile potentiation of section, it is possible to decrease the environmental pollution that excessive fertilization or improper fertilization etc. are caused；
(2) Y- polyglutamic acids can make crop more effectively absorb the nutrient in soil, promote crop root development, reach volume increase and carry
Matter, the effect for strengthening resistances against diseases；(3) Y- polyglutamic acids have good chela for heavy metal ion such as lead ion, copper ions
Effect is closed, can be used as heavy metals absorbent；(4) Y- polyglutamic acids have preferable buffer capacity to acid, alkali, can active balance
The pH-value of soil, it is to avoid the soil property acidifying caused because of chronic administration chemical fertilizer and soil compaction.Accordingly, as a kind of green bio
Macromole, Y- polyglutamic acids are the biological environmental production type fertilizer synergists of great application potential.
It is also of common occurrence with regard to the patent of Y- polyglutamic acids in recent years, but not yet occur combining Y- polyglutamic acids and crops
The invention of straw natural organic fertilizer.Contain a large amount of organic matters, nitrogen, phosphorus, potassium and trace element in agricultural crop straw, but it is most of
Agricultural crop straw by directly discard, burn, cause the huge waste to resource and the severe contamination to environment.The present invention with
Agricultural crop straw with low cost is raw material, adds microorganism fungus kind, the rotten fermentation of Jing high temperature nuclear reactors, then is gathered with tall and erect effect composition Y- with it
Glutamic acid makes high-quality fertilizer.The fertilizer nutrient content is balanced, containing nitrogen, phosphorus, potassium a great number of elements, abundant trace element and has
Beneficial microorganism flora, along with the potentiation of Y- polyglutamic acids, to improved soil, culture fertility, improves crop quality and has
Good action.Therefore, from from the point of view of efficient utilization natural regeneration resource and raising fertilizer utilization efficiency, crops straw are researched and developed
The novel environment friendly fertilizer that stalk is combined with Y- polyglutamic acids, returns can sufficient amount of organic matter, nitrogen, phosphorus, potassium and trace element
Return soil, to promote ecosystem in biological cycle, reach the purpose for keeping and strengthening soil fertility, especially this product with the addition of
Y- polyglutamic acids, the effect with more preferable volume increase, water conservation, fertilizer conservation and improved soil has for sustainable high-efficiency agriculture is developed
There is the important and meaning of reality.
The content of the invention
It is an object of the invention to provide a kind of preparation method of the agricultural crop straw fertilizer containing Y- polyglutamic acids, the method
With agricultural crop straw as base material, microorganism fungus kind, the rotten fermentation of Jing high temperature nuclear reactors are added, then composition Y- polyglutamic acids are imitated with tall and erect with it,
The agricultural crop straw fertilizer containing Y- polyglutamic acids is obtained, soil fertility can be effectively improved, improve crop growth environment.
It is another object of the present invention to the agricultural crop straw fertilizer containing Y- polyglutamic acids of the present invention is applied to into soil
In earth fertilising.
The present invention is achieved by the following technical solutions：
The preparation method of the agricultural crop straw fertilizer containing Y- polyglutamic acids of the present invention, specifically includes following steps:
(1) it is inoculated with the preparation of microbial inoculum
The cellulose that 4 DEG C of inclined-planes are preserved and the activation of lignin fungi bacterial strain, under 55 DEG C of condition of culture, use culture medium
Culture 3-7d, takes 1.5-2L bacterium solutions and be centrifuged under 10000r/min rotating speeds and cultivate 10-30min, finally by 1L fibre-bearings and wooden
The culture fluid of plain decomposer uniformly mixes with 3kg Testa oryzaes, obtains being inoculated with microbial inoculum；
(2) preparation of agricultural crop straw granule
Agricultural crop straw is crushed with pulverizer, rotary screen or vibrosieve screening is crossed, uniform agricultural crop straw is obtained
Grain；
(3) composting of fermenting crops straws pile
First agricultural crop straw granule obtained in l00kg steps (2) is laid in into ground, on its surface lkg steps are spread respectively
(1) obtained inoculation microbial inoculum, lkg carbamide and lkg magnesium sulfate, are then laid in l00kg agricultural crop straw granules above, then
The obtained inoculation microbial inoculum of lkg steps (1), lkg carbamide and lkg magnesium sulfate, such step process 1000kg agriculture are spread respectively in its surface
Crop material granule, is obtained fermenting crops straws pile；
(4) fermentation of During High-Temperature Composting
600kg water is sprayed on fermenting crops straws pile obtained in step (3), it is then thin in its surface plastic covering
Film, the time of banking up is 30d, obtains agricultural crop straw fertilizer；
(5) addition of Y- polyglutamic acids
Agricultural crop straw fertilizer 200-500 mass parts, the throwing of Y- polyglutamic acid 50-100 mass parts that step (4) is obtained
Enter and uniformly mixed in U-shaped mixer, make the agricultural crop straw fertilizer containing Y- polyglutamic acids.
Preferably, agricultural crop straw of the present invention is wheat straw, Caulis et Folium Oryzae, corn straw, broomcorn straw, Cotton Gossypii straw
The combination of one or more in stalk, Caulis Sacchari sinensis straw.
Preferably, the cellulose and lignin fungi described in step (1) is Hypocrea virens, Trichoderma lignorum bacterium, rope
The combination of one or more in shape penicillium, white rot fungi, soft-rot fungi, chaetomium thermophilum.
Preferably, the composition of step (1) culture medium, by percentage to the quality for：1% yeast powder, 2% Fructus Vitis viniferae
Sugar, 0.2% cellulose, 0.2% lignin, remaining is water, and pH is 7.0-7.2.
Preferably, during entirely the banking up of step (4), in banking up, 5d, 10d, 15d and 20d open plastic sheeting
Dry in the sun 0.5 day, prevents the too high suppression microbial activitiess of temperature, promotes moisture to scatter and disappear as early as possible.
Preferably, can be with the addition Jenner that in step (5) so as to which quality is identical in quality with Y- polyglutamic acids,
You are the fertilizer additives containing various trace elements and bioactive enzyme to described Jenner, can be effectively increased crop yield.
Agricultural crop straw fertilizer containing Y- polyglutamic acids prepared by the present invention can be applicable to greengrocery, grain class and economy
In the soil application of crop.
Beneficial effects of the present invention are as follows：
(1) preparation method of the agricultural crop straw fertilizer containing Y- polyglutamic acids of the invention is with agricultural crop straw as base
Material, adds microorganism fungus kind, the rotten fermentation of Jing high temperature nuclear reactors, then polyglutamic acid containing Y- is obtained with tall and erect effect composition Y- polyglutamic acids with it
Agricultural crop straw fertilizer, the preparation process is simple of the present invention, low cost, controllability is high, the crops containing Y- polyglutamic acids
Straw manures yield height, non-environmental-pollution, fertilizer efficiency are high.
(2) present invention prepares the agricultural crop straw fertilizer containing Y- polyglutamic acids, a side with agricultural crop straw as base material
Face reduces agricultural crop straw and directly discards, burns the huge waste caused to resource and the severe contamination caused to environment, separately
Contain a large amount of organic matters, nitrogen, phosphorus, potassium and trace element in one side agricultural crop straw, be the organic fat of substrate manufacture with it
Effect is high, can effectively improve the yield of crop.
(3) agricultural crop straw is natural regeneration resource, research and development agricultural crop straw and Y- polyglutamic acids with Y- polyglutamic acids
With reference to novel environment friendly fertilizer, sufficient amount of organic matter, nitrogen, phosphorus, potassium and trace element can be made to return soil, to promote life
Biological cycle in state system, reaches the purpose for keeping and strengthening soil fertility, and especially this product with the addition of Y- polyglutamic acids, have
The more preferable effect of volume increase, water conservation, fertilizer conservation and improved soil, for the sustainable high-efficiency agriculture of development has the important and meaning of reality
Justice.
Specific embodiment
The above of the present invention is described in further detail below by way of specific embodiment, but this should not be understood
Scope for above-mentioned theme of the invention is only limitted to below example.Without departing from the idea case in the present invention described above, root
The various replacements made according to ordinary skill knowledge and customary means or change, all should be included within the scope of the invention.
Embodiment 1
The preparation of the agricultural crop straw base natural organic fertilizer containing Y- polyglutamic acids of the present embodiment, specifically includes following step
Suddenly:
(1) it is inoculated with the preparation of microbial inoculum
The Trichoderma viride bacteria strain activation that 4 DEG C of inclined-planes are preserved, under 55 DEG C of condition of culture, uses culture medium culturing 7d, takes
2L bacterium solutions are centrifuged culture 30min under 10000r/min rotating speeds, finally that the culture fluid of 1L Hypocrea virens is uniform with 3kg Testa oryzaes
Mixing, obtains being inoculated with microbial inoculum；
(2) preparation of wheat straw granule
Wheat straw is crushed with pulverizer, rotary screen screening is crossed, uniform wheat straw granule is obtained；
(3) composting of wheat straw fermentation pile
First wheat straw granule obtained in l00kg steps (2) is laid in into ground, spreads lkg steps (1) respectively on its surface and be obtained
Inoculation microbial inoculum, lkg carbamide and lkg magnesium sulfate, then l00kg wheat straw granules are laid in above, then spread respectively on its surface
Lkg inoculations microbial inoculum, lkg carbamide and lkg magnesium sulfate, such step process 1000kg wheat straw granule, are obtained wheat obtained in step (1)
Straw fermentation pile；
(4) fermentation of During High-Temperature Composting
600kg water is sprayed on wheat straw fermentation pile obtained in step (3), then in its surface plastic covering thin film, is banked up
Time is 30d, obtains wheat straw fertilizer；
(5) addition of Y- polyglutamic acids
In the mass parts of wheat straw fertilizer 500, the U-shaped mixer of the mass parts of Y- polyglutamic acids 100 input that step (4) is obtained
Uniformly mixed, made the wheat straw fertilizer containing Y- polyglutamic acids.
The composition of the present embodiment step (1) culture medium, by percentage to the quality for：1% yeast powder, 2% glucose,
0.2% cellulose, 0.2% lignin, remaining is water, and pH is 7.0-7.2.
The present embodiment is opened plastic sheeting and is dried in the air during entirely the banking up of step (4) in bank up 5d, 10d, 15d and 20d
Shine 0.5 day.
The wheat straw fertilizer containing Y- polyglutamic acids for preparing the present invention is tested, in being applied to the soil application of Fructus Lycopersici esculenti, and
Impose carbamide to contrast, each processes three and repeats, make each process amount of nitrogen equal (12kg/ mus).Above-mentioned process can make Fructus Lycopersici esculenti
Growing way is vigorous, and blade is thickening to enlarge afforested area, and tamato fruit is big full, compared with the check plot Fructus Lycopersici esculenti for applying carbamide, the Fructus Lycopersici esculenti of trial zone
Volume increase 21%.
Embodiment 2
The preparation of the agricultural crop straw base natural organic fertilizer containing Y- polyglutamic acids of the present embodiment, specifically includes following step
Suddenly:
(1) it is inoculated with the preparation of microbial inoculum
The Trichoderma lignorum bacterium that 4 DEG C of inclined-planes are preserved and the activation of penicillium funiculosum bacteria strain, under 55 DEG C of condition of culture, with culture
Base culture 3d, takes 1.5L bacterium solutions and culture 10min is centrifuged under 10000r/min rotating speeds, finally that 1L Trichoderma lignorums bacterium and rope form is blue or green
The culture fluid of mycete uniformly mixes with 3kg Testa oryzaes, obtains being inoculated with microbial inoculum；
(2) preparation of Caulis et Folium Oryzae granule
Caulis et Folium Oryzae is crushed with pulverizer, vibrosieve screening is crossed, uniform Caulis et Folium Oryzae granule is obtained；
(3) composting of rice straw fermented pile
First Caulis et Folium Oryzae granule obtained in l00kg steps (2) is laid in into ground, spreads lkg steps (1) respectively on its surface and be obtained
Inoculation microbial inoculum, lkg carbamide and lkg magnesium sulfate, then l00kg Caulis et Folium Oryzae granules are laid in above, then spread respectively on its surface
Lkg inoculations microbial inoculum, lkg carbamide and lkg magnesium sulfate, such step process 1000kg Caulis et Folium Oryzae granule, are obtained rice obtained in step (1)
Grass fermentation pile；
(4) fermentation of During High-Temperature Composting
600kg water is sprayed on rice straw fermented pile obtained in step (3), then in its surface plastic covering thin film, is banked up
Time is 30d, obtains Caulis et Folium Oryzae fertilizer；
(5) addition of Y- polyglutamic acids
The mass parts of Caulis et Folium Oryzae fertilizer 200, the mass parts of Y- polyglutamic acids 50, Jenner's 50 mass parts of that step (4) is obtained
Put in U-shaped mixer and uniformly mixed, make the Caulis et Folium Oryzae fertilizer containing Y- polyglutamic acids.
The composition of the present embodiment step (1) culture medium, by percentage to the quality for：1% yeast powder, 2% glucose,
0.2% cellulose, 0.2% lignin, remaining is water, and pH is 7.0-7.2.
The present embodiment is opened plastic sheeting and is dried in the air during entirely the banking up of step (4) in bank up 5d, 10d, 15d and 20d
Shine 0.5 day.
The Caulis et Folium Oryzae fertilizer containing Y- polyglutamic acids for preparing the present invention is tested, in being applied to the soil application of Chinese cabbage,
And impose carbamide and contrast, each processes three and repeats, and makes each process amount of nitrogen equal (12kg/ mus).Above-mentioned process can make greatly
Chinese cabbage growing way is vigorous, and blade is thickening to enlarge afforested area, compared with the check plot Chinese cabbage for applying carbamide, the Chinese cabbage volume increase 31% of trial zone.
Embodiment 3
The preparation of the agricultural crop straw base natural organic fertilizer containing Y- polyglutamic acids of the present embodiment, specifically includes following step
Suddenly:
(1) it is inoculated with the preparation of microbial inoculum
The white rot fungi that 4 DEG C of inclined-planes are preserved and the activation of soft-rot fungi bacterial strain, under 55 DEG C of condition of culture, use culture medium culturing
5d, take 2L bacterium solutions be centrifuged under 10000r/min rotating speeds culture 20min, finally by the culture fluid of 1L white rot fungi and soft-rot fungi with
3kg Testa oryzaes uniformly mix, and obtain being inoculated with microbial inoculum；
(2) preparation of corn stalk pelletses
Corn straw is crushed with pulverizer, rotary screen screening is crossed, uniform corn stalk pelletses are obtained；
(3) composting of corn straw fermentation pile
First corn stalk pelletses obtained in l00kg steps (2) are laid in into ground, on its surface lkg steps (1) are spread respectively
Obtained inoculation microbial inoculum, lkg carbamide and lkg magnesium sulfate, are then laid in l00kg corn stalk pelletses above, then in its table
Lkg inoculations microbial inoculum, lkg carbamide and lkg magnesium sulfate, such step process 1000kg corn stalk obtained in step (1) are spread respectively in face
Stalk granule, is obtained corn straw fermentation pile；
(4) fermentation of During High-Temperature Composting
600kg water is sprayed on corn straw fermentation pile obtained in step (3), then in its surface plastic covering thin film,
The time bank up for 30d, corn straw fertilizer is obtained；
(5) addition of Y- polyglutamic acids
The mass parts of corn straw fertilizer 300, the U-shaped mixing of the mass parts of Y- polyglutamic acids 70 input that step (4) is obtained
Uniformly mixed in machine, made the corn straw fertilizer containing Y- polyglutamic acids.
The composition of the present embodiment step (1) culture medium, by percentage to the quality for：1% yeast powder, 2% glucose,
0.2% cellulose, 0.2% lignin, remaining is water, and pH is 7.0-7.2.
The present embodiment is opened plastic sheeting and is dried in the air during entirely the banking up of step (4) in bank up 5d, 10d, 15d and 20d
Shine 0.5 day.
The corn straw fertilizer containing Y- polyglutamic acids for preparing the present invention is tested, the soil application of Semen arachidis hypogaeae is applied to
In, and impose carbamide and contrast, each processes three and repeats, and makes each process amount of nitrogen equal (12kg/ mus).Above-mentioned process can make
Semen arachidis hypogaeae growing way is vigorous, and peanut fruit is full greatly, compared with the check plot Semen arachidis hypogaeae for applying carbamide, the peanut yield increasing 25% of trial zone.
Embodiment 4
The preparation of the agricultural crop straw base natural organic fertilizer containing Y- polyglutamic acids of the present embodiment, specifically includes following step
Suddenly:
(1) it is inoculated with the preparation of microbial inoculum
The Chaetomium thermophile bacteria strain activation that 4 DEG C of inclined-planes are preserved, under 55 DEG C of condition of culture, uses culture medium culturing 4d, takes
1.8L bacterium solutions are centrifuged culture 15min under 10000r/min rotating speeds, finally that the culture fluid of 1L chaetomium thermophilums is equal with 3kg Testa oryzaes
Even mixing, obtains being inoculated with microbial inoculum；
(2) preparation of broomcorn straw granule
Broomcorn straw is crushed with pulverizer, vibrosieve screening is crossed, uniform broomcorn straw stalk particle is obtained；
(3) composting of broomcorn straw fermentation pile
First broomcorn straw granule obtained in l00kg steps (2) is laid in into ground, on its surface lkg steps (1) are spread respectively
Obtained inoculation microbial inoculum, lkg carbamide and lkg magnesium sulfate, are then laid in l00kg broomcorn straw granules above, then in its table
Lkg inoculations microbial inoculum, lkg carbamide and lkg magnesium sulfate, such step process 1000kg sorghum stalks obtained in step (1) are spread respectively in face
Stalk granule, is obtained broomcorn straw fermentation pile；
(4) fermentation of During High-Temperature Composting
600kg water is sprayed on broomcorn straw fermentation pile obtained in step (3), then in its surface plastic covering thin film,
The time bank up for 30d, broomcorn straw fertilizer is obtained；
(5) addition of Y- polyglutamic acids
The mass parts of broomcorn straw fertilizer 300, the U-shaped mixing of the mass parts of Y- polyglutamic acids 80 input that step (4) is obtained
Uniformly mixed in machine, made the broomcorn straw fertilizer containing Y- polyglutamic acids.
The composition of the present embodiment step (1) culture medium, by percentage to the quality for：1% yeast powder, 2% glucose,
0.2% cellulose, 0.2% lignin, remaining is water, and pH is 7.0-7.2.
The present embodiment is opened plastic sheeting and is dried in the air during entirely the banking up of step (4) in bank up 5d, 10d, 15d and 20d
Shine 0.5 day.
The broomcorn straw fertilizer containing Y- polyglutamic acids for preparing the present invention is tested, the soil application of Folium Camelliae sinensis is applied to
In, and impose carbamide and contrast, each processes three and repeats, and makes each process amount of nitrogen equal (12kg/ mus).Above-mentioned process can make
Folium Camelliae sinensis growing way is vigorous, and blade is thickening to enlarge afforested area, compared with the check plot Folium Camelliae sinensis for applying carbamide, the Folium Camelliae sinensis volume increase 19% of trial zone.Implement
Example 5
The preparation of the agricultural crop straw base natural organic fertilizer containing Y- polyglutamic acids of the present embodiment, specifically includes following step
Suddenly:
(1) it is inoculated with the preparation of microbial inoculum
Hypocrea virens, Trichoderma lignorum bacterium and the activation of penicillium funiculosum bacteria strain that 4 DEG C of inclined-planes are preserved, at 55 DEG C bar is cultivated
Under part, culture medium culturing 5d is used, take 2L bacterium solutions and culture 30min is centrifuged under 10000r/min rotating speeds, finally by 1L Trichoderma virides
The culture fluid of bacterium, Trichoderma lignorum bacterium and penicillium funiculosum uniformly mixes with 3kg Testa oryzaes, obtains being inoculated with microbial inoculum；
(2) preparation of cotton stalk granule
Cotton stalk is crushed with pulverizer, rotary screen screening is crossed, uniform cotton stalk granule is obtained；
(3) composting of cotton stalk fermentation pile
First cotton stalk granule obtained in l00kg steps (2) is laid in into ground, on its surface lkg steps (1) are spread respectively
Obtained inoculation microbial inoculum, lkg carbamide and lkg magnesium sulfate, are then laid in l00kg cotton stalk granules above, then in its table
Lkg inoculations microbial inoculum, lkg carbamide and lkg magnesium sulfate, such step process 1000kg Cotton Gossypii straw obtained in step (1) are spread respectively in face
Stalk granule, is obtained cotton stalk fermentation pile；
(4) fermentation of During High-Temperature Composting
600kg water is sprayed on cotton stalk fermentation pile obtained in step (3), then in its surface plastic covering thin film,
The time bank up for 30d, cotton stalk fertilizer is obtained；
(5) addition of Y- polyglutamic acids
The mass parts of cotton stalk fertilizer 500, the mass parts of Y- polyglutamic acids 100, the Jenner's that 100 that step (4) is obtained
Mass parts put in U-shaped mixer and are uniformly mixed, and make the cotton stalk fertilizer containing Y- polyglutamic acids.
The composition of the present embodiment step (1) culture medium, by percentage to the quality for：1% yeast powder, 2% glucose,
0.2% cellulose, 0.2% lignin, remaining is water, and pH is 7.0-7.2.
The present embodiment is opened plastic sheeting and is dried in the air during entirely the banking up of step (4) in bank up 5d, 10d, 15d and 20d
Shine 0.5 day.
The cotton stalk fertilizer containing Y- polyglutamic acids for preparing the present invention is tested, the soil application of Semen Tritici aestivi is applied to
In, and impose carbamide and contrast, each processes three and repeats, and makes each process amount of nitrogen equal (12kg/ mus).Above-mentioned process can make
Semen Tritici aestivi growing way is vigorous, full grains, compared with the check plot Semen Tritici aestivi for applying carbamide, the wheat increase yield 31% of trial zone.Embodiment 6
The preparation of the agricultural crop straw base natural organic fertilizer containing Y- polyglutamic acids of the present embodiment, specifically includes following step
Suddenly:
(1) it is inoculated with the preparation of microbial inoculum
White rot fungi, soft-rot fungi and the activation of Chaetomium thermophile bacteria strain that 4 DEG C of inclined-planes are preserved, under 55 DEG C of condition of culture,
Culture medium culturing 5d is used, 2L bacterium solutions is taken and culture 20min is centrifuged under 10000r/min rotating speeds, finally by 1L white rot fungi, soft corruption
The culture fluid of bacterium and chaetomium thermophilum uniformly mixes with 3kg Testa oryzaes, obtains being inoculated with microbial inoculum；
(2) preparation of Caulis Sacchari sinensis stalk particle
Caulis Sacchari sinensis straw is crushed with pulverizer, vibrosieve screening is crossed, uniform Caulis Sacchari sinensis stalk particle is obtained；
(3) composting of Caulis Sacchari sinensis stalk fermentation pile
First Caulis Sacchari sinensis stalk particle obtained in l00kg steps (2) is laid in into ground, on its surface lkg steps (1) are spread respectively
Obtained inoculation microbial inoculum, lkg carbamide and lkg magnesium sulfate, are then laid in l00kg Caulis Sacchari sinensis stalk particles above, then in its table
Lkg inoculations microbial inoculum, lkg carbamide and lkg magnesium sulfate, such step process 1000kg Caulis Sacchari sinensis straw obtained in step (1) are spread respectively in face
Stalk granule, is obtained fermenting crops straws pile；
(4) fermentation of During High-Temperature Composting
600kg water is sprayed on Caulis Sacchari sinensis stalk fermentation pile obtained in step (3), then in its surface plastic covering thin film,
The time bank up for 30d, Caulis Sacchari sinensis Straw manures are obtained；
(5) addition of Y- polyglutamic acids
The mass parts of Caulis Sacchari sinensis Straw manures 300, the U-shaped mixing of the mass parts of Y- polyglutamic acids 100 input that step (4) is obtained
Uniformly mixed in machine, made the Caulis Sacchari sinensis Straw manures containing Y- polyglutamic acids.
The composition of the present embodiment step (1) culture medium, by percentage to the quality for：1% yeast powder, 2% glucose,
0.2% cellulose, 0.2% lignin, remaining is water, and pH is 7.0-7.2.
The present embodiment is opened plastic sheeting and is dried in the air during entirely the banking up of step (4) in bank up 5d, 10d, 15d and 20d
Shine 0.5 day.
The Caulis Sacchari sinensis Straw manures containing Y- polyglutamic acids for preparing the present invention are tested, the soil application of Semen Maydiss is applied to
In, and impose carbamide and contrast, each processes three and repeats, and makes each process amount of nitrogen equal (12kg/ mus).Above-mentioned process can make
Semen Maydiss growing way is vigorous, and grain is full greatly, compared with the check plot Semen Maydiss for applying carbamide, the corn yield increasing 23% of trial zone.
Claims (8)
1. a kind of preparation method of the agricultural crop straw fertilizer containing Y- polyglutamic acids, it is characterised in that specifically include following step
Suddenly：
(1) it is inoculated with the preparation of microbial inoculum
The cellulose that 4 DEG C of inclined-planes are preserved and the activation of lignin fungi bacterial strain, under 55 DEG C of condition of culture, use culture medium culturing
3-7d, takes 1.5-2L bacterium solutions and culture 10-30min is centrifuged under 10000r/min rotating speeds, finally by 1L fibre-bearings and lignin point
The culture fluid of solution bacterium uniformly mixes with 3kg Testa oryzaes, obtains being inoculated with microbial inoculum；
(2) preparation of agricultural crop straw granule
Agricultural crop straw is crushed with pulverizer, rotary screen or vibrosieve screening is crossed, uniform agricultural crop straw granule is obtained；
(3) composting of fermenting crops straws pile
First agricultural crop straw granule obtained in l00kg steps (2) is laid in into ground, on its surface lkg steps (1) system is spread respectively
Inoculation microbial inoculum, lkg carbamide and the lkg magnesium sulfate for obtaining, is then laid in l00kg agricultural crop straw granules above, then in its table
The obtained inoculation microbial inoculum of lkg steps (1), lkg carbamide and lkg magnesium sulfate, such step process 1000kg crops are spread respectively in face
Stalk particle, is obtained fermenting crops straws pile；
(4) fermentation of During High-Temperature Composting
600kg water is sprayed on fermenting crops straws pile obtained in step (3), then in its surface plastic covering thin film, heap
The time is put for 30d, agricultural crop straw fertilizer is obtained；
(5) addition of Y- polyglutamic acids
Agricultural crop straw fertilizer 200-500 mass parts, the Y- polyglutamic acid 50-100 mass parts input U that step (4) is obtained
Uniformly mixed in type mixer, made the agricultural crop straw fertilizer containing Y- polyglutamic acids.
2. the preparation method of the agricultural crop straw fertilizer containing Y- polyglutamic acids according to claim 1, it is characterised in that：
Described agricultural crop straw is the one kind or several in wheat straw, Caulis et Folium Oryzae, corn straw, broomcorn straw, cotton stalk, Caulis Sacchari sinensis straw
The combination planted.
3. the preparation method of the agricultural crop straw fertilizer containing Y- polyglutamic acids according to claim 1, it is characterised in that：
Cellulose and lignin fungi described in step (1) be Hypocrea virens, Trichoderma lignorum bacterium, penicillium funiculosum, white rot fungi,
The combination of one or more in soft-rot fungi, chaetomium thermophilum.
4. the preparation method of the agricultural crop straw fertilizer containing Y- polyglutamic acids according to claim 1, it is characterised in that：
The composition of step (1) culture medium, by percentage to the quality for：1% yeast powder, 2% glucose, 0.2% cellulose,
0.2% lignin, remaining is water, and pH is 7.0-7.2.
5. the preparation method of the agricultural crop straw fertilizer containing Y- polyglutamic acids according to claim 1, it is characterised in that：
During entirely the banking up of step (4), plastic sheeting dry in the sun is opened 0.5 day in bank up 5d, 10d, 15d and 20d.
6. the preparation method of the agricultural crop straw fertilizer containing Y- polyglutamic acids according to claim 1, it is characterised in that：
Can be with the addition Jenner that in step (5).
7. the preparation method of the agricultural crop straw fertilizer containing Y- polyglutamic acids according to claim 6, it is characterised in that：
Your quality of the Jenner is identical in quality with Y- polyglutamic acids.
8. a kind of application of the agricultural crop straw fertilizer containing Y- polyglutamic acids, it is characterised in that：This contains the agriculture of Y- polyglutamic acids
Crop material fertilizer can be applicable in the soil application of greengrocery, grain class and industrial crops.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611088448.9A CN106588303A (en) | 2016-12-01 | 2016-12-01 | Preparation method and application of crop straw organic fertilizer containing poly-Y-glutamicacid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611088448.9A CN106588303A (en) | 2016-12-01 | 2016-12-01 | Preparation method and application of crop straw organic fertilizer containing poly-Y-glutamicacid |
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| Publication Number | Publication Date |
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| CN106588303A true CN106588303A (en) | 2017-04-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611088448.9A Withdrawn CN106588303A (en) | 2016-12-01 | 2016-12-01 | Preparation method and application of crop straw organic fertilizer containing poly-Y-glutamicacid |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107540461A (en) * | 2017-10-19 | 2018-01-05 | 荆楚理工学院 | A kind of ecological organic fertilier and its production method with soil improvement function |
| CN108314587A (en) * | 2018-03-19 | 2018-07-24 | 广西中艾农业科技有限公司 | A kind of tulip special cultivation soil |
| CN110845253A (en) * | 2019-11-20 | 2020-02-28 | 中国科学院成都生物研究所 | Preparation and application method of gamma-polyglutamic acid biological organic fertilizer |
| CN113234446A (en) * | 2021-04-02 | 2021-08-10 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | Biomass saline-alkali soil conditioner |
| CN113261468A (en) * | 2021-06-11 | 2021-08-17 | 陕西美好家园农业科技发展股份有限公司 | Apple tree planting method mainly based on farmyard manure |
-
2016
- 2016-12-01 CN CN201611088448.9A patent/CN106588303A/en not_active Withdrawn
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107540461A (en) * | 2017-10-19 | 2018-01-05 | 荆楚理工学院 | A kind of ecological organic fertilier and its production method with soil improvement function |
| CN107540461B (en) * | 2017-10-19 | 2020-09-22 | 荆楚理工学院 | Ecological organic fertilizer with soil improvement function and production method thereof |
| CN108314587A (en) * | 2018-03-19 | 2018-07-24 | 广西中艾农业科技有限公司 | A kind of tulip special cultivation soil |
| CN110845253A (en) * | 2019-11-20 | 2020-02-28 | 中国科学院成都生物研究所 | Preparation and application method of gamma-polyglutamic acid biological organic fertilizer |
| CN110845253B (en) * | 2019-11-20 | 2022-02-08 | 中国科学院成都生物研究所 | Preparation and application method of gamma-polyglutamic acid biological organic fertilizer |
| CN113234446A (en) * | 2021-04-02 | 2021-08-10 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | Biomass saline-alkali soil conditioner |
| CN113261468A (en) * | 2021-06-11 | 2021-08-17 | 陕西美好家园农业科技发展股份有限公司 | Apple tree planting method mainly based on farmyard manure |
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Application publication date: 20170426 |