CN101974426B - Preparation method for lignocellulose decomposition composite flora and application thereof - Google Patents
Preparation method for lignocellulose decomposition composite flora and application thereof Download PDFInfo
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- CN101974426B CN101974426B CN2010102821620A CN201010282162A CN101974426B CN 101974426 B CN101974426 B CN 101974426B CN 2010102821620 A CN2010102821620 A CN 2010102821620A CN 201010282162 A CN201010282162 A CN 201010282162A CN 101974426 B CN101974426 B CN 101974426B
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- cow dung
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- cow
- panaeolus
- coprinus
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- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 239000002131 composite material Substances 0.000 title claims abstract description 15
- 238000000354 decomposition reaction Methods 0.000 title claims abstract description 14
- 241000222511 Coprinus Species 0.000 claims abstract description 18
- 241001236144 Panaeolus Species 0.000 claims abstract description 18
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 230000004151 fermentation Effects 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 13
- 239000002361 compost Substances 0.000 claims abstract description 12
- 239000001963 growth medium Substances 0.000 claims abstract description 12
- 241000235527 Rhizopus Species 0.000 claims abstract description 10
- 241000223259 Trichoderma Species 0.000 claims abstract description 10
- 239000000725 suspension Substances 0.000 claims abstract description 7
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 4
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 210000003608 fece Anatomy 0.000 claims description 35
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 14
- 239000002023 wood Substances 0.000 claims description 11
- 229920002522 Wood fibre Polymers 0.000 claims description 8
- 244000005700 microbiome Species 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000002054 inoculum Substances 0.000 claims description 6
- 238000012807 shake-flask culturing Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 241000228212 Aspergillus Species 0.000 claims 2
- 238000007873 sieving Methods 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 1
- 239000010902 straw Substances 0.000 abstract description 9
- 238000009264 composting Methods 0.000 abstract description 5
- 238000002156 mixing Methods 0.000 abstract description 4
- 241000123346 Chrysosporium Species 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 229920005610 lignin Polymers 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000003413 degradative effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to a preparation method for a lignocellulose decomposition composite flora and application thereof, discloses a method which can prepare a high-efficiency lignocellulose decomposition composite flora rapidly, simply and conveniently, in particular provides a microbial inoculum for the composting of cow muck and agricultural straw, and solves the problem that the cow muck and the agricultural straw are difficult to compost. The composite flora is formed by mixing fermentation liquor obtained by fermenting a cow muck culture medium and spore suspension of phanerochete chrysosporium and trichoderma sp, rhizopus sp, panaeolus sp and coprinus sp which are separated from the cow muck, and can completely degrade the cow muck and the agricultural straw of lignocellulose. By using the microbial inoculum in the composting of the cow muck and the straw, the fermentation period is as short as only 25 days. The preparation method for the flora is simple and is easy to implement; the flora content in each unit of raw materials is high; and the activity is high.
Description
Technical field
The present invention relates to a kind of preparation method and its usage of wood fibre decomposition composite flora, is a kind of biotechnology of utilizing microbiological treatment agriculture waste stalk.
Technical background
China is a cowboying big country, and in recent years, to mass-producing, intensive future development, cow dung becomes more and more serious to the pollution problem of environment along with cattle-raising.The Chinese agriculture statistical yearbook showed in 1999, and the number of animals raised of ox is 3506.94 ten thousand/year, and every ox produces 10.6 ton/years of excrement amounts, produces 4.9 ton/years of urine amounts; Total 13689.52 ten thousand ton/years of excrement amounts of producing, always produce 4500.66 ten thousand ton/years of urine amounts.Composting process needs three or four months half a year even for the cold cow dung of property, lasts length, and processing efficiency is low, is difficult to adapt to modern breeding scale industry development.The temperature of traditional cow dung heap is not high yet, its use value that has been difficult to kill the drawbacks limit such as a large amount of weeds neutrons contained in cow dung and worm's ovum germ.How processing rapidly and effectively cow dung is the new challenge that the scientific worker faces.
Fresh cow dung water ratio, all more than 80%, is usually regulated by adding the agricultural crop straws such as wheat straw, rice husk.In agricultural stalk, be xylogen than the organic composition of difficult degradation, its degraded in composting is the key that promotes the composting process.The degraded of xylogen can provide a new thinking and technology for the efficiency that improves compost, the research that the shortening compost time is improved compost quality.
At occurring in nature, can lignin degrading and the biology that produces corresponding enzyme only occupy the minority.The fungi of lignin degrading is mainly white-rot fungi, and this type of fungi can secrete born of the same parents' external oxidation enzyme liberating xylogen, therefore is considered to topmost lignin degradation microorganism.At present, the stalk fermentation technology generally adopted both at home and abroad such as aerobic fermentation or anaerobically fermenting exist more single such as bacterial classification, and a little less than capacity of decomposition, Mierocrystalline cellulose total degradation rate is low, be generally 20～40% left and right and fermentation period longer, generally need the problems such as fermentation period of 50d left and right.
Summary of the invention
The preparation method and its usage that the purpose of this invention is to provide a kind of wood fibre decomposition composite flora, for solving, have that the microbiobacterial agent kind of utilizing is single now in the straw degradative process, a little less than capacity of decomposition, the problem such as Mierocrystalline cellulose total degradation rate is low and fermentation period is long, a kind of highly effective straw decomposition composite flora is provided.
The object of the present invention is achieved like this:
The preparation method of wood fibre decomposition composite flora of the present invention carries out according to following step:
(1) preparation of cow dung culture medium: take in the beaker that cow dung 150g adds 1000mL, pour 400mL water into, fully stir 30min, filter and take down slag system, get in the triangular flask that 100mL adds 250mL stand-by;
(2) shake-flask culture Inoculant preparation: get in the beaker that cow dung compost 150g is added to the 1000mL that contains 400mL water, fully stir 30min, stand-by;
(3) get in the 250mL triangular flask that 10mL shake-flask culture Inoculant is added to 100mL liquid cow dung culture medium, be placed in rotating speed and be 120r/min and temperature and be on the shaking table of 45 ℃ and cultivate 3～5d;
(4) Phanerochaete chrysosporium (Phanerochaete chrysosporium) and the cultivation of the ordinary method that adopts microorganism to separate from the upper wood mould (Trichoderma sp) separated of cow dung, head mold (Rhizopus sp), Panaeolus fungi (Panaeolus sp), For Form of Coprinus Fungies (Coprinus sp).By the bacterial classification of Phanerochaete chrysosporium (Phanerochaete chrysosporium) and wood mould (Trichodermasp), head mold (Rhizopus sp), Panaeolus fungi (Panaeolus sp), For Form of Coprinus Fungies (Coprinus sp) respectively through containing the slant culture of PDA substratum, obtain its spore, concentration is every milliliter 1 * 10
5Individual spore, wherein PDA substratum: adopt the potato leach liquor of 200g/L, then add the agar of the glucose of 20g/L and 17～20g/L and be dissolved to 1L;
(5) will adopt fermented liquid that the fermentation of cow dung culture medium obtains and Phanerochaete chrysosporium (Phanerochaete chrysosporium) and adopt ordinary method that microorganism separates from above wood mould (Trichoderma sp), head mold (Rhizopus sp), Panaeolus fungi (Panaeolus sp), the spore suspension mixing of For Form of Coprinus Fungies (Coprinus sp) of separation of cow dung, the volume ratio of several bacterium liquid is 2: 1: 1: 1: 1: 1, then dry, obtain fermenting agent.
The purposes of wood fibre decomposition composite flora of the present invention is using this kind microbiobacterial agent as cow dung and the compost fermentation microbial inoculum of agricultural stalk.
The present invention has the following advantages: the lignocellulose in 1, can comprehensively degrade various organic substances, especially stalk and cow dung, and degradation rate is high, and the cycle is short, lower about 25 days of suitable condition, degradation rate reaches more than 60%; 2, can realize effective utilization of agricultural wastes and feces of livestock and poultry, improvement agricultural, aquaculture ecotope, reduce the atmospheric pollution and the protection of the environment that because of crop straw burning, cause.
Embodiment:
The preparation method of embodiment 1 wood fibre decomposition composite flora of the present invention:
By following concrete steps, prepare:
(1) preparation of cow dung culture medium: take in the beaker that cow dung 150g adds 1000mL, pour 400mL water into, fully stir 30min, filter and take down slag system, get in the triangular flask that 100mL adds 250mL stand-by;
(2) shake-flask culture Inoculant preparation: get cow dung compost 40g, sieve, then, in taking 20g and being added to the beaker of the 1000mL that contains 50mL water, fully stir 30min, stand-by;
(3) get in the 250mL triangular flask that 10mL shake-flask culture Inoculant is added to 100mL liquid cow dung culture medium, be placed in rotating speed and be 120r/min and temperature and be on the shaking table of 45 ℃ and cultivate 3d～5d;
(4) Phanerochaete chrysosporium (Phanerochaete chrysosporium) and the cultivation of the ordinary method that adopts microorganism to separate from the upper wood mould (Trichoderma sp) separated of cow dung, head mold (Rhizopus sp), Panaeolus sp, Coprinus sp.By the bacterial classification of Phanerochaete chrysosporium (Phanerochaete chrysosporium) and wood mould (Trichoderma sp), head mold (Rhizopus sp), Panaeolus fungi (Panaeolus sp), For Form of Coprinus Fungies (Coprinus sp) respectively through containing the slant culture of PDA substratum, obtain its spore, concentration is every milliliter 1 * 10
5Individual spore, wherein PDA substratum: adopt the potato leach liquor of 200g/L, then add the agar of the glucose of 20g/L and 17～20g/L and be dissolved to 1L;
(5) will adopt fermented liquid that the fermentation of cow dung culture medium obtains and Phanerochaete chrysosporium (Phanerochaete chrysosporium) and adopt ordinary method that microorganism separates from above wood mould (Trichoderma sp), head mold (Rhizopus sp), Panaeolus fungi (Panaeolus sp), the spore suspension mixing of For Form of Coprinus Fungies (Coprinus sp) of separation of cow dung, the volume ratio of bacterium liquid is 2: 1: 1: 1: 1: 1.Drying, obtain fermenting agent.
The fermented liquid that the fermentation of embodiment 2 cow dung culture mediums obtains and Phanerochaete chrysosporium (Phanerochaetechrysosporium) and preparation such as the embodiment 1 of the ordinary method that adopts microorganism to separate from the spore suspension mixing of the upper wood mould (Trichoderma sp) separated of cow dung, head mold (Rhizopussp), Panaeolus fungi (Panaeolus sp), For Form of Coprinus Fungies (Coprinus sp).
This composite fungus agent is applicable to stalk and ight soil is the compost use of main raw material.
Preparation such as embodiment 1 that the ordinary method that the fermented liquid that the fermentation of embodiment 3 cow dung culture mediums obtains separates with Phanerochaete chrysosporium (Phanerochaete chrysosporium) and employing microorganism is mixed from the upper wood mould (Trichoderma sp) separated of cow dung, head mold (Rhizopus sp) spore suspension.
The fermented liquid that the fermentation of adopting cow dung culture medium is obtained mixes with the spore suspension of Phanerochaete chrysosporium (Phanerochaete chrysosporium), wood mould (Trichoderma) and head mold (Rhizopus sp), and the volume ratio of three kinds of bacterium liquid is 1: 2: 1.This composite fungus agent is applicable to stalk (bacterium liquid is that the volume ratio of 1 centimetre of left and right stalk adopts 1: 100-1 with pulverizing: 200, the two fully mixes, humidity is controlled at 75-80%) to be used for the compost of main raw material.
Claims (2)
1. the preparation method of a wood fibre decomposition composite flora, is characterized in that, carries out according to following step:
(1). the preparation of cow dung culture medium: take in the beaker that cow dung 150g adds 1000mL, pour 400mL water into, fully stir 30min, filter and take down slag system, get in the triangular flask that 100mL adds 250mL stand-by;
(2). shake-flask culture Inoculant preparation: get and take the compost 40g that cow dung adopts ordinary method to bank up and obtain as raw material, after sieving, and then the compost after sieving takes in the beaker that 20g is added to the 1000mL that contains 50mL water, fully stirs 30min, stand-by;
(3). get in the 250mL triangular flask that 10mL shake-flask culture Inoculant is added to 100mL liquid cow dung culture medium, be placed in rotating speed and be 120r/min and temperature and be on the shaking table of 45 ℃ and cultivated 3 days～5 days;
(4). Phanerochaete chrysosporium (Phanerochaete chrysosporium) and adopt the cultivation from the upper wood mould (Trichoderma sp) separated of cow dung, head mold (Rhizopus sp), aspergillus (Aspergillus sp), Panaeolus fungi (Panaeolus sp), For Form of Coprinus Fungies (Coprinus sp) of ordinary method that microorganism separates: the bacterial classification of Phanerochaete chrysosporium and wood is mould, head mold, Panaeolus fungi, For Form of Coprinus Fungies process respectively contains the PDA culture medium culturing, obtain its spore, concentration is every milliliter 1 * 10
8Individual spore, wherein PDA substratum: adopt the potato leach liquor of 200g/L, then add the agar of the glucose of 20g/L and 17～20g/L and be dissolved to 1L;
(5). will adopt the spore suspension of fermented liquid that the fermentation of cow dung culture medium obtains and Phanerochaete chrysosporium and wooden mould, head mold, Panaeolus fungi, For Form of Coprinus Fungies to mix, the volume ratio of bacterium liquid is 2: 1: 1: 1: 1: 1, then dry, obtain fermenting agent.
2. right to use requires the application of 1 described wood fibre decomposition composite flora as the compost fermentation microbial inoculum of cow dung and agricultural stalk.
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| CN101974426B true CN101974426B (en) | 2013-11-27 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102120970A (en) * | 2010-11-29 | 2011-07-13 | 昆明理工大学 | Preparation method and application of wood fiber decomposed composite flora |
| CN102199564B (en) * | 2011-03-28 | 2012-11-21 | 武汉合缘绿色生物工程有限公司 | Composite microbial agent for direct decomposition of crop straws returned to field and preparation method thereof |
| CN103352013B (en) * | 2013-06-21 | 2014-11-26 | 南京工业大学 | Lignocellulose degrading complex bacterium system and application |
| CN106905006A (en) * | 2017-02-23 | 2017-06-30 | 华南理工大学 | A kind of method for carrying out sludge aerobic compost using high temperature bacterium and white-rot fungi synergy |
| CN108059991A (en) * | 2017-08-21 | 2018-05-22 | 重庆大雨水生态科技有限公司 | A kind of New-type fuel and brick, haydite, cement, fiberboard based on heavy metal sewage sludge |
| CN108315269A (en) * | 2018-05-04 | 2018-07-24 | 重庆市农业技术推广总站 | A kind of waste dish promotees rotten microbial inoculum and its preparation and application |
| CN112593437A (en) * | 2020-12-23 | 2021-04-02 | 合肥工业大学 | Biodegradation method of lignin |
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| CN101560488A (en) * | 2009-05-27 | 2009-10-21 | 中国农业大学 | Enzyme and microbial inoculum for decomposing lignocellulose |
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| CN101560488A (en) * | 2009-05-27 | 2009-10-21 | 中国农业大学 | Enzyme and microbial inoculum for decomposing lignocellulose |
| CN101597580A (en) * | 2009-06-01 | 2009-12-09 | 中国农业大学 | The method of lignocellulose degradation and special cellulose thereof decompose fungus strain |
| CN101717728A (en) * | 2009-12-28 | 2010-06-02 | 华东理工大学 | Penicillium and application thereof in catalyzing and hydrolyzing lignocellulose |
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