CN102120970A - Preparation method and application of wood fiber decomposed composite flora - Google Patents
Preparation method and application of wood fiber decomposed composite flora Download PDFInfo
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- CN102120970A CN102120970A CN2010105623871A CN201010562387A CN102120970A CN 102120970 A CN102120970 A CN 102120970A CN 2010105623871 A CN2010105623871 A CN 2010105623871A CN 201010562387 A CN201010562387 A CN 201010562387A CN 102120970 A CN102120970 A CN 102120970A
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- cow dung
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- panaeolus
- coprinus
- fungi
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- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000002131 composite material Substances 0.000 title claims abstract description 15
- 229920002522 Wood fibre Polymers 0.000 title claims abstract description 11
- 239000002025 wood fiber Substances 0.000 title abstract 3
- 210000003608 fece Anatomy 0.000 claims abstract description 41
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims abstract description 22
- 241000222511 Coprinus Species 0.000 claims abstract description 17
- 241001236144 Panaeolus Species 0.000 claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 241000235527 Rhizopus Species 0.000 claims abstract description 13
- 239000002361 compost Substances 0.000 claims abstract description 13
- 239000001963 growth medium Substances 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 12
- 239000002023 wood Substances 0.000 claims abstract description 11
- 239000000725 suspension Substances 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 14
- 241000223259 Trichoderma Species 0.000 claims description 11
- 238000000354 decomposition reaction Methods 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 244000005700 microbiome Species 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000002054 inoculum Substances 0.000 claims description 6
- 238000012807 shake-flask culturing Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 239000002068 microbial inoculum Substances 0.000 claims description 2
- 238000007873 sieving Methods 0.000 claims 2
- 241000228212 Aspergillus Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 239000010902 straw Substances 0.000 abstract description 9
- 238000009264 composting Methods 0.000 abstract description 4
- 229920002678 cellulose Polymers 0.000 abstract description 3
- 239000001913 cellulose Substances 0.000 abstract description 3
- 238000002156 mixing Methods 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 1
- 230000000855 fungicidal effect Effects 0.000 abstract 1
- 239000000417 fungicide Substances 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 3
- 229920005610 lignin Polymers 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000003413 degradative effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a preparation method and application of wood fiber decomposed composite flora. The preparation method is a quick, simple and convenient method for preparing the high-efficiency wood fiber decomposed composite flora. The preparation method is specifically used for providing microbial fungicide for composting of cow dung and agricultural straws, and solving the problem that the cow dung and the agricultural straws are hard to compost. The composite flora is formed by mixing fermentation liquor with a spore suspension consisting of Phanerochaete chrysosporium, Trichoderam sp separated from cow dung, Rhizopus sp, Panaeolus sp and Coprinus sp, wherein the fermentation liquor is obtained by fermenting a cow dung culture medium, and the cow dung and the agricultural straw of wood cellulose can be comprehensively decomposed. The microbial preparation is used in the compost of the cow dung and the straw, and the fermentation period is short and is 25 days. The preparation method of the flora is simple and can be implemented easily; and the content of the flora in unit raw material is high, and the activity of the flora is high.
Description
Technical field
The present invention relates to a kind of preparation method and its usage of wood fibre decomposition composite flora, is a kind of biotechnology of utilizing microbiological treatment agriculture waste stalk.
Background technology
China is a cowboying big country, and in recent years, along with cattle-raising develops to mass-producing, intensification direction, cow dung becomes more and more serious to the pollution problem of environment.The Chinese agriculture statistical yearbook showed in 1999, and the number of animals raised of ox is 3506.94 ten thousand/year, and every ox produces 10.6 tons/year of excrement amounts, produces 4.9 tons/year of urine amounts; 13689.52 ten thousand tons/year of gross output excrement amounts, gross output urine are measured 4500.66 ten thousand tons/year.Composting process needs three or four months even half a year for the cold cow dung of property, lasts length, and processing efficiency is low, is difficult to adapt to modern breeding scale and already develops.The temperature of traditional cow dung heap is not high yet, its use value that has been difficult to kill drawbacks limit such as a large amount of weeds neutrons contained in the cow dung and worm's ovum germ.How handling cow dung rapidly and effectively is the new challenge that the scientific worker faces.
Fresh cow dung water ratio is usually regulated by adding agricultural crop straws such as wheat straw, rice husk all more than 80%.Organic composition than difficult degradation in the agricultural stalk is an xylogen, and its degraded in composting is the key that promotes the composting process.The degraded of xylogen can provide a new thinking and technology for the efficient that improves compost, the research that the shortening compost time is improved compost quality.
At occurring in nature, can lignin degrading and the biology that produces corresponding enzyme only occupy the minority.The fungi of lignin degrading mainly is a white-rot fungi, and this type of fungi can secrete born of the same parents' external oxidation enzyme liberating xylogen, therefore is considered to topmost lignin degradation microorganism.At present, stalk fermentation technology that generally adopts such as aerobic fermentation or anaerobically fermenting existence both at home and abroad is more single such as bacterial classification, and a little less than the capacity of decomposition, Mierocrystalline cellulose total degradation rate is low, be generally about 20～40% and fermentation period longer, generally need the problems such as fermentation period about 50d.
Summary of the invention
The preparation method and its usage that the purpose of this invention is to provide a kind of wood fibre decomposition composite flora, the microbiobacterial agent kind that has now in the straw degradative process to be utilized is single in order to solve, a little less than the capacity of decomposition, problem such as Mierocrystalline cellulose total degradation rate is low and fermentation period is long, a kind of highly effective straw decomposition composite flora is provided.
The object of the present invention is achieved like this:
The preparation method of wood fibre decomposition composite flora of the present invention carries out according to following step:
(1) preparation of cow dung culture medium: take by weighing cow dung 150g and add in the beaker of 1000mL, pour 400mL water into, fully stir 30min, filter and take down slag system, get in the triangular flask that 100mL adds 250mL stand-by;
(2) shake-flask culture Inoculant preparation: get in the beaker that cow dung compost 150g is added to the 1000mL that contains 400mL water, fully stir 30min, stand-by;
(3) get in the 250mL triangular flask that 10mL shake-flask culture Inoculant is added to 100mL liquid cow dung culture medium, placing rotating speed is that 120r/min and temperature are to cultivate 3～5d on 45 ℃ the shaking table;
(4) Phanerochaete chrysosporium (Phanerochaete chrysosporium) and the isolating ordinary method of employing microorganism go up the cultivation of isolating wood mould (Trichoderma sp), head mold (Rhizopus sp), Panaeolus fungi (Panaeolus sp), Coprinus fungi (Coprinus sp) from cow dung.With the bacterial classification of Phanerochaete chrysosporium (Phanerochaete chrysosporium) and wood mould (Trichodermasp), head mold (Rhizopus sp), Panaeolus fungi (Panaeolus sp), Coprinus fungi (Coprinus sp) respectively through containing the slant culture of PDA substratum, obtain its spore, concentration is every milliliter 1 * 10
5Individual spore, wherein PDA substratum: adopt the potato leach liquor of 200g/L, add the glucose of 20g/L and the agar of 17～20g/L then and also decide molten to 1L;
(5) will adopt the fermented liquid of the fermentation acquisition of cow dung culture medium to mix with the spore suspension that Phanerochaete chrysosporium (Phanerochaete chrysosporium) and the isolating ordinary method of employing microorganism go up isolating wood mould (Trichoderma sp), head mold (Rhizopus sp), Panaeolus fungi (Panaeolus sp), Coprinus fungi (Coprinus sp) from cow dung, the volume ratio of several bacterium liquid is 2: 1: 1: 1: 1: 1, dry then, obtain fermenting agent.
The purposes of wood fibre decomposition composite flora of the present invention is with the compost fermentation microbial inoculum of this kind microbiobacterial agent as cow dung and agricultural stalk.
The present invention has the following advantages: the lignocellulose in 1, can comprehensively degrade various organic substances, especially stalk and the cow dung, and the degradation rate height, the cycle is short, following about 25 days of suitable condition, degradation rate reaches more than 60%; 2, can realize effective utilization of agricultural wastes and feces of livestock and poultry, improvement agricultural, aquaculture ecotope reduce the atmospheric pollution and the protection environment that cause because of crop straw burning.
Embodiment:
The preparation method of embodiment 1 wood fibre decomposition composite flora of the present invention:
Prepare by following concrete steps:
(1) preparation of cow dung culture medium: take by weighing cow dung 150g and add in the beaker of 1000mL, pour 400mL water into, fully stir 30min, filter and take down slag system, get in the triangular flask that 100mL adds 250mL stand-by;
(2) shake-flask culture Inoculant preparation: get cow dung compost 40g, sieve, be added in the beaker of the 1000mL that contains 50mL water taking by weighing 20g then, fully stir 30min, stand-by;
(3) get in the 250mL triangular flask that 10mL shake-flask culture Inoculant is added to 100mL liquid cow dung culture medium, placing rotating speed is that 120r/min and temperature are to cultivate 3d～5d on 45 ℃ the shaking table;
(4) Phanerochaete chrysosporium (Phanerochaete chrysosporium) and the isolating ordinary method of employing microorganism are from the upward cultivation of isolating wood mould (Trichoderma sp), head mold (Rhizopus sp), Panaeolus sp, Coprinus sp of cow dung.With the bacterial classification of Phanerochaete chrysosporium (Phanerochaete chrysosporium) and wood mould (Trichoderma sp), head mold (Rhizopus sp), Panaeolus fungi (Panaeolus sp), Coprinus fungi (Coprinus sp) respectively through containing the slant culture of PDA substratum, obtain its spore, concentration is every milliliter 1 * 10
5Individual spore, wherein PDA substratum: adopt the potato leach liquor of 200g/L, add the glucose of 20g/L and the agar of 17～20g/L then and also decide molten to 1L;
(5) will adopt the fermented liquid of the fermentation acquisition of cow dung culture medium to mix with the spore suspension that Phanerochaete chrysosporium (Phanerochaete chrysosporium) and the isolating ordinary method of employing microorganism go up isolating wood mould (Trichoderma sp), head mold (Rhizopus sp), Panaeolus fungi (Panaeolus sp), Coprinus fungi (Coprinus sp) from cow dung, the volume ratio of bacterium liquid is 2: 1: 1: 1: 1: 1.Drying obtains fermenting agent.
Fermented liquid that the fermentation of embodiment 2 cow dung culture mediums obtains and Phanerochaete chrysosporium (Phanerochaete chrysosporium) and the spore suspension blended that adopts the isolating ordinary method of microorganism to go up isolating wood mould (Trichoderma sp), head mold (Rhizopus sp), Panaeolus fungi (Panaeolus sp), Coprinus fungi (Coprinus sp) from cow dung prepare as embodiment 1.
This composite fungus agent is fit to stalk and ight soil is the compost use of main raw material.
Upward isolating wood mould (Trichoderma sp), head mold (Rhizopus sp) spore suspension blended prepare as embodiment 1 from cow dung for fermented liquid that the fermentation of embodiment 3 cow dung culture mediums obtains and Phanerochaete chrysosporium (Phanerochaete chrysosporium) and the isolating ordinary method of employing microorganism.
The fermented liquid that the fermentation of adopting cow dung culture medium is obtained mixes with the spore suspension of Phanerochaete chrysosporium (Phanerochaete chrysosporium), wood mould (Trichoderma) and head mold (Rhizopus sp), and the volume ratio of three kinds of bacterium liquid is 1: 2: 1.This composite fungus agent is fit to stalk (bacterium liquid is that the volume ratio of 1 centimetre of left and right sides stalk adopts 1: 100-1 with pulverizing: 200, the two thorough mixing, humidity is controlled at 75-80%) to be used for the compost of main raw material.
Claims (2)
1. the preparation method of a wood fibre decomposition composite flora is characterized in that, carries out according to following step:
(1) preparation of cow dung culture medium: take by weighing cow dung 150g and add in the beaker of 1000mL, pour 400mL water into, fully stir 30min, filter and take down slag system, get in the triangular flask that 100mL adds 250mL stand-by;
(2) shake-flask culture Inoculant preparation: getting with the cow dung is the compost 40g that raw material adopts ordinary method to bank up and obtain, and after sieving, and then the compost after will sieving takes by weighing 20g and be added in the beaker of the 1000mL that contains 50mL water, fully stirs 30min, and is stand-by;
(3) get in the 250mL triangular flask that 10mL shake-flask culture Inoculant is added to 100mL liquid cow dung culture medium, placing rotating speed is that 120r/min and temperature are to cultivate 3d～5d on 45 ℃ the shaking table;
(4) Phanerochaete chrysosporium Phanerochaete chrysosporium and the isolating ordinary method of employing microorganism go up the mould Trichoderma sp of isolating wood from cow dung, head mold Rhizopus sp, aspergillus Aspergillus sp, Panaeolus fungi Panaeolus sp, the cultivation of Coprinus fungi Coprinus sp: with Phanerochaete chrysosporium Phanerochaete chrysosporium and Trichoderma sp, head mold Rhizopus sp, Panaeolus fungi Panaeolus sp, the bacterial classification of Coprinus fungi Coprinus sp is respectively through containing the PDA culture medium culturing, obtain its spore, concentration is every milliliter 1 * 10
8Individual spore, wherein PDA substratum: adopt the potato leach liquor of 200g/L, add the glucose of 20g/L and the agar of 17～20g/L then and also decide molten to 1L;
(5) will adopt the spore suspension of fermented liquid that the fermentation of cow dung culture medium obtains and Phanerochaete chrysosporium Phanerochaete chrysosporium and Trichoderma sp, head mold Rhizopus sp, Panaeolus fungi Panaeolus sp, Coprinus fungi Coprinus sp to mix, the volume ratio of bacterium liquid is 2: 1: 1: 1: 1: 1, dry then, obtain fermenting agent.
2. the purposes of the described wood fibre decomposition composite flora of claim 1 is characterized in that: with the compost fermentation microbial inoculum of this kind microbiobacterial agent as cow dung and agricultural stalk.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010105623871A CN102120970A (en) | 2010-11-29 | 2010-11-29 | Preparation method and application of wood fiber decomposed composite flora |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105623871A CN102120970A (en) | 2010-11-29 | 2010-11-29 | Preparation method and application of wood fiber decomposed composite flora |
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| CN102120970A true CN102120970A (en) | 2011-07-13 |
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| CN2010105623871A Pending CN102120970A (en) | 2010-11-29 | 2010-11-29 | Preparation method and application of wood fiber decomposed composite flora |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102409008A (en) * | 2011-09-02 | 2012-04-11 | 单茂莹 | Microbial agent for straw decomposition into field |
| CN103352013A (en) * | 2013-06-21 | 2013-10-16 | 南京工业大学 | Lignocellulose degrading complex bacterium system and application |
| CN106905006A (en) * | 2017-02-23 | 2017-06-30 | 华南理工大学 | A kind of method for carrying out sludge aerobic compost using high temperature bacterium and white-rot fungi synergy |
| CN112314392A (en) * | 2020-10-28 | 2021-02-05 | 甘肃省科学院生物研究所 | Process for preparing soil adjusting matrix by solid-state enhanced fermentation of garden waste |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560488A (en) * | 2009-05-27 | 2009-10-21 | 中国农业大学 | Enzyme and microbial inoculum for decomposing lignocellulose |
| CN101974426A (en) * | 2010-09-15 | 2011-02-16 | 昆明理工大学 | Preparation method for lignocellulose decomposition composite flora and application thereof |
-
2010
- 2010-11-29 CN CN2010105623871A patent/CN102120970A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560488A (en) * | 2009-05-27 | 2009-10-21 | 中国农业大学 | Enzyme and microbial inoculum for decomposing lignocellulose |
| CN101974426A (en) * | 2010-09-15 | 2011-02-16 | 昆明理工大学 | Preparation method for lignocellulose decomposition composite flora and application thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102409008A (en) * | 2011-09-02 | 2012-04-11 | 单茂莹 | Microbial agent for straw decomposition into field |
| CN103352013A (en) * | 2013-06-21 | 2013-10-16 | 南京工业大学 | Lignocellulose degrading complex bacterium system and application |
| CN103352013B (en) * | 2013-06-21 | 2014-11-26 | 南京工业大学 | Lignocellulose degrading complex bacterium system and application |
| CN106905006A (en) * | 2017-02-23 | 2017-06-30 | 华南理工大学 | A kind of method for carrying out sludge aerobic compost using high temperature bacterium and white-rot fungi synergy |
| CN112314392A (en) * | 2020-10-28 | 2021-02-05 | 甘肃省科学院生物研究所 | Process for preparing soil adjusting matrix by solid-state enhanced fermentation of garden waste |
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Application publication date: 20110713 |