CN108611293B - Formula and preparation method of bio-enzyme strain in bio-enzyme pulping process - Google Patents

Formula and preparation method of bio-enzyme strain in bio-enzyme pulping process Download PDF

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CN108611293B
CN108611293B CN201810369415.4A CN201810369415A CN108611293B CN 108611293 B CN108611293 B CN 108611293B CN 201810369415 A CN201810369415 A CN 201810369415A CN 108611293 B CN108611293 B CN 108611293B
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CN108611293A (en
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李鑫
李树泉
刘学勇
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Shanghai Kaichen Industrial Co ltd
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Abstract

The invention discloses a formula of bio-enzyme strains in a bio-enzyme pulping process, which consists of strain components and enzyme components in percentage by weight as follows: bacillus, bifidobacterium, lactic acid bacteria, microzyme, trichoderma reesei, aspergillus niger complex enzyme preparation, cellulase, laccase, protease, high-temperature amylase, lignin degrading enzyme, phytase, glucanase, pectinase, EDTA, vitamin C, glycine, cysteine and sodium chloride. The invention also discloses a preparation method thereof, the activity of each component enzyme is high, the unit cost is low, the fermentation period is short, the industrialization degree is high, the preparation method is applied to papermaking and pulping, no equipment is required to be added, the process is simple, the use is convenient, compared with the traditional chemical pulping method, the COD content in the wastewater is reduced by more than 35%, the production cost is reduced by about 22%, and the product quality of the paper is improved.

Description

Formula and preparation method of bio-enzyme strain in bio-enzyme pulping process
Technical Field
The invention belongs to the technical field of biological enzyme pulping, and particularly relates to a formula and a preparation method of a biological enzyme strain in a biological enzyme pulping process.
Background
China is a big agricultural country, biomass resources are extremely rich, crop straws can be used for producing paper pulp, various biological enzymes are applied to treat cellulose, lignin and hemicellulose in the straws at a proper temperature, the cellulose is degraded and softened, the hemicellulose, pectin and saccharides are quickly converted into other soluble and volatile substances, and the biological enzymes have softening effect on the lignin, so that certain lignin can be degraded, and the burden of water treatment is reduced on the premise of not influencing papermaking.
However, in the traditional pulping methods such as an alkaline method, an ammonium sulfite method, a sodium sulfite method and the like, besides a large amount of energy consumption in the pulping process, a large amount of black liquor is generated in the pulping cooking process, so that expensive water treatment equipment is required to be added, the papermaking cost is increased, and the construction recovery period is prolonged. With the development and the gradual improvement of compound biological enzymology and microecology in the technical field of biological enzyme pulping, the biological enzyme pulping additive is more and more widely applied to the pulping and papermaking industry. The compound biological enzyme pulping and papermaking additive is a preparation of active compound biological enzyme and metabolite thereof which are beneficial to the environment, has the characteristics of no toxicity, no residue and no pollution, and has important significance for environmental protection. At present, the research and development of the formula of the biological enzyme strains in the domestic and foreign biological enzyme pulping process are mainly concentrated on three types: one kind is a living bacteria preparation represented by bacillus subtilis and yeast culture, which contains active substances for the growth of composite biological enzyme and active ingredients such as living bacteria, and can effectively improve the degradation efficiency of the fiber lignin; one kind is enzyme preparation represented by protease, amylase, lipase and cellulase, which takes compound biological enzyme with high enzyme production capacity as culture strain, obtains enzyme substance through fermentation process, and the enzyme preparation can directly act on plant fiber to promote degradation of fiber and remove lignin; one is the biological enzyme strain formula in the pulping process of composite biological enzyme of live bacteria and enzyme, and has various functions of micro-ecological regulation and promotion of pulping and papermaking.
Disclosure of Invention
The invention provides a formula and a preparation technology of bio-enzyme strains in a bio-enzyme pulping process, which can improve the competitive capacity of beneficial bacteria in a fiber pulping process, effectively balance the activities of flora and bio-enzyme, promote the action capacity on plant fiber pulping and papermaking, reduce the papermaking production cost and environmental pollution when being applied to papermaking and pulping, and simultaneously contribute to improving the product quality of paper.
A formula of bio-enzyme strains in a bio-enzyme pulping process is composed of strain components and enzyme components, and comprises the following components in parts by weight: -1% -5.5% of bacillus, 3% -10% of bifidobacterium, 5% -15% of lactic acid bacteria, 5% -8% of yeast, 6% -8% of trichoderma reesei, 10% -16% of an aspergillus niger complex enzyme preparation, 3% -6% of cellulase, 6% -8% of laccase, 4% of protease-7%, 5% -8% of high-temperature amylase, 2% -5% of lignin-degrading enzyme, 6% -12% of phytase, 6% -12% of glucanase, 3% -5% of pectinase, 0.1% -0.2% of EDTA, 0.1% -0.5% of vitamin C, 0.01% -0.1% of glycine, 0.2% -0.3% of cysteine, 1% -1.5% of sodium chloride, the viable bacteria content of which is no less than 8 × cfu/g, 1000U/ml of cellulase, 900U/ml/1200U/ml, protease 00U/ml, 00U/ml of high-temperature amylase, 4005% of lignin-5%, 6% -12% of phytase, 6% of glucanase, 0.1% -0, 1000U/ml of lignin degrading enzyme, 1000-1500U/ml of phytase, 5000U/ml of glucanase and 800-1200U/ml of pectinase.
Preferably, the formula of the bio-enzyme strain in the bio-enzyme pulping process comprises the following components in parts by weight: 3.5% of bacillus, 8% of bifidobacterium, 10% of lactic acid bacteria, 6% of saccharomycetes, 7% of trichoderma reesei, 12% of aspergillus niger complex enzyme preparation, 5% of cellulase, 6.5% of laccase, 6% of protease, 8% of high-temperature amylase, 4.95% of lignin degrading enzyme, 7% of phytase, 9% of glucanase, 5% of pectinase, 0.2% of EDTA, 0.3% of vitamin C, 0.05% of glycine, 0.3% of cysteine and 1.2% of sodium chloride, wherein the viable count content of the microbial inoculum is more than or equal to 8-109cfu/g。
The invention contains more beneficial bacteria and functional enzymes, and respectively prepares the live bacterial preparation of the culture by fermentation: adopting wheat bran, corn, bean cypress and other raw materials as fermentation enzyme production culture media of bacillus, bifidobacterium, lactobacillus, saccharomycete and photosynthetic bacteria, and preparing a complex enzyme preparation by compatibility; the live bacteria preparation and the complex enzyme preparation are compounded according to a proper proportion to prepare a double-effect additive product.
Solid fermentation culture: taking a sterilized solid fermentation culture medium, cooling to 40-45 ℃ in a fermentation workshop subjected to disinfection treatment, diluting and uniformly mixing cultured aspergillus niger liquid seeds with distilled water, uniformly spraying the diluted and uniformly mixed aspergillus niger liquid seeds onto the solid fermentation culture medium, uniformly mixing the fermentation culture medium, and flatly paving the mixture on a fermentation bed, wherein the thickness of the mixture is 3-5 cm, the temperature of the mixture is 28-32 ℃, and the relative humidity of the mixture is 80-90%; turning over the bed once every 12 hours, and properly spraying distilled water; co-culturing for 48 hours; and then, air-drying the fermentation material to obtain the aspergillus niger complex enzyme preparation.
The live bacteria preparation is prepared by respectively inoculating a bacillus, a bifidobacterium, a lactic acid bacterium, a saccharomycete, a trichoderma reesei and an aspergillus niger complex enzyme preparation into wheat bran and bean-cypress enzymolysis saccharification liquid, performing 10L of automatic seed fermentation on 5L of a canned seed culture medium, culturing for 20 hours at 28-32 ℃ according to the inoculation amount of 5%, and drying fermentation liquid at a low temperature of 40-50 ℃.
The mass ratio of the bran to the water in the mixture of bran and water used for fermentation in the fermentation tank is 2:3, the bran having a particle size of 2mm to 8 mm; or the mass ratio of the wheat bran to the water in the mixture of the wheat bran and the water used for fermentation in the fermentation tank is 1:2, and the wheat bran powder has a particle size of 4mm to 5 mm.
Adding 1% oligosaccharide as a protective agent into the fermentation liquor of bacillus, bifidobacterium, lactobacillus, saccharomycetes, trichoderma reesei and aspergillus niger, and drying at low temperature of 40-50 ℃ respectively to prepare the bacillus, bifidobacterium, lactobacillus and saccharomycetes.
The enzyme preparation is prepared by spraying liquid seeds of cellulase, laccase, protease, high temperature amylase, lignin-degrading enzyme, phytase, glucanase and pectinase on a mixture of 60% wheat bran, 30% bean-shaped cypress and 0.4% KH2PO4In the solid culture medium, the fermentation culture medium is uniformly stirred and is flatly paved on a fermentation bed, the thickness is about 3-5 cm, the temperature is 28-32 ℃, and the relative humidity is 80-90%; the bed is turned over once every 12 hours, and distilled water can be properly sprayed; co-culturing for 48 hours; then the fermentation material is dried in the air, and an enzyme preparation compounded by cellulase, laccase, protease, high-temperature amylase, lignin degrading enzyme, phytase, glucanase and pectinase is obtained.
The live bacteria preparation and the complex enzyme preparation are compounded according to the proportion, and simultaneously, the buffer solution is added to prepare the double-effect additive product.
The paper making raw material is pretreated and then sent into a digester for enzyme treatment, namely, the raw material is soaked in the compound enzyme solution. By adopting the technical scheme, the invention better realizes the aim, has high activity of each component enzyme, low unit cost, short fermentation period and high industrialization degree, is applied to papermaking and pulping, does not need to increase equipment, has simple process and convenient use, reduces the COD content in the wastewater by more than 35 percent and the production cost by about 22 percent compared with the traditional chemical pulping method, and improves the product quality of paper.
(1) The invention contains beneficial bacteria, biological enzyme and functional substance oligosaccharide, the beneficial bacteria and the biological enzyme in the formula can improve the utilization rate of fiber pulping and papermaking in a short time and promote the production performance through the decomposition of viable bacteria and the biological catalysis, and the utilization rate of rough pulp papermaking can be improved after the product of the invention is used in actual production.
(2) The invention does not contain anaerobic bacteria, does not need an anaerobic culture process, has low requirements on production conditions, saves related equipment investment and production links, has simple preparation process and low cost, and is suitable for industrial production.
(3) According to the invention, the bacillus subtilis is adopted as the viable bacteria preparation strain, the strain is fast to reproduce, the culture time of the viable bacteria preparation can be saved, and the production efficiency is improved; in the drying process of the viable bacteria preparation, oligosaccharide is used as a protective agent, so that the production cost is further reduced, the viable bacteria loss in the preparation process of the viable bacteria preparation can be obviously reduced, the requirements on pulping and papermaking can be met, the effect of pulping and papermaking additives in a pulping machine is enhanced, the beneficial flora can be effectively improved and balanced, the pulping cost is reduced, and the production performance is improved.
Detailed Description
The following examples are given for the detailed implementation and specific operation of the present invention, but the scope of the present invention is not limited to the following examples.
Example 1
A formula of bio-enzyme strains in a bio-enzyme pulping process is composed of strain components and enzyme components, and comprises the following components in parts by weight: 3.5% of bacillus, 8% of bifidobacterium, 10% of lactic acid bacteria, 6% of saccharomycetes, 7% of trichoderma reesei, 12% of aspergillus niger complex enzyme preparation, 5% of cellulase, 6.5% of laccase, 6% of protease, 8% of high-temperature amylase, 4.95% of lignin degrading enzyme, 7% of phytase, 9% of glucanase, 5% of pectinase, 0.2% of EDTA, 0.3% of vitamin C, 0.05% of glycine, 0.3% of cysteine and 1.2% of sodium chloride, wherein the viable count content of the microbial inoculum is more than or equal to 8-109cfu/g。
The preparation method comprises the following steps:
solid fermentation culture: taking a sterilized solid fermentation culture medium, cooling to 40-45 ℃ in a fermentation workshop subjected to disinfection treatment, diluting and uniformly mixing cultured aspergillus niger liquid seeds with distilled water, uniformly spraying the diluted and uniformly mixed aspergillus niger liquid seeds onto the solid fermentation culture medium, uniformly mixing the fermentation culture medium, and flatly paving the mixture on a fermentation bed, wherein the thickness of the mixture is 4 cm, the temperature of the mixture is 30 ℃, and the relative humidity of the mixture is 82%; turning over the bed once every 12 hours, and properly spraying distilled water; co-culturing for 48 hours; and then, air-drying the fermentation material to obtain the aspergillus niger complex enzyme preparation.
The live bacteria preparation is prepared by respectively inoculating bacillus, bifidobacterium, lactobacillus, saccharomycetes, trichoderma reesei and aspergillus niger complex enzyme preparation into wheat bran and bean-cypress enzymolysis saccharification liquid, automatically fermenting 10L seeds and canning 5L seed culture medium, culturing at 30 ℃ for 20 hours according to the inoculation amount of 5%, and respectively drying fermentation liquor at low temperature of 42 ℃.
The mass ratio of bran to water in the mixture of bran and water used for fermentation in the fermentation tank is 2:3, the bran has a particle size of 6 mm;
the bacillus, the bifidobacterium, the lactobacillus, the saccharomycete, the trichoderma reesei and the aspergillus niger fermentation liquor are respectively prepared by adding 1 percent of oligosaccharide as a protective agent and then drying at a low temperature of 45 ℃.
The enzyme preparation is prepared by spraying liquid seeds containing cellulase, laccase, protease, high temperature amylase, lignin-degrading enzyme and pectinase on wheat bran 60%, bean-tree cypress 30% and KH 0.4%2PO4The solid culture medium is evenly stirred and spread on a fermentation bed, the thickness is about 4 cm, the temperature is 30 ℃, and the relative humidity is 85 percent; the bed is turned over once every 12 hours, and distilled water can be properly sprayed; co-culturing for 48 hours; then the fermentation material is dried in the air, and an enzyme preparation compounded by cellulase, laccase, protease, high-temperature amylase, lignin degrading enzyme and pectinase is obtained.
Example 2
A formula of bio-enzyme strains in a bio-enzyme pulping process is composed of strain components and enzyme components, and comprises the following components in parts by weight: 5.5 percent of bacillus, 10 percent of bifidobacterium, 5 percent of lactic acid bacteria, 5 percent of microzyme, 8 percent of trichoderma reesei and 13 percent of aspergillus niger complex enzyme preparation,6% of cellulase, 8% of laccase, 4% of protease, 5% of high-temperature amylase, 2% of lignin degrading enzyme, 12% of phytase, 12% of glucanase, 3% of pectinase, 0.1% of EDTA (ethylene diamine tetraacetic acid), 0.14% of vitamin C, 0.01% of glycine, 0.25% of cysteine and 1% of sodium chloride, wherein the viable count content of the microbial inoculum is more than or equal to 8 x 109cfu/g。
The preparation method is the same as example 1.
Example 3
A formula of bio-enzyme strains in a bio-enzyme pulping process is composed of strain components and enzyme components, and comprises the following components in parts by weight: 4% of bacillus, 6% of bifidobacterium, 6% of lactic acid bacteria, 8% of saccharomycetes, 8% of trichoderma reesei, 16% of aspergillus niger complex enzyme preparation, 6% of cellulase, 8% of laccase, 7% of protease, 5% of high-temperature amylase, 3.6% of lignin degrading enzyme, 10% of phytase, 7% of glucanase, 3% of pectinase, 0.1% of EDTA, 0.5% of vitamin C, 0.1% of glycine, 0.2% of cysteine and 1.5% of sodium chloride, wherein the viable count content of the microbial inoculum is more than or equal to 8 x 109cfu/g。
The preparation method is the same as example 1.
Example 4
A formula of bio-enzyme strains in a bio-enzyme pulping process is composed of strain components and enzyme components, and comprises the following components in parts by weight: 3.5% of bacillus, 8% of bifidobacterium, 10% of lactic acid bacteria, 6% of microzyme, 7% of trichoderma reesei, 12% of aspergillus niger complex enzyme preparation, 5% of cellulase, 6.5% of laccase, 6% of protease, 8% of high-temperature amylase, 5% of lignin degrading enzyme, 9% of phytase, 9% of glucanase and 5% of pectinase, wherein the viable count content of the microbial inoculum is more than or equal to 8 x 109cfu/g。
The preparation method is the same as example 1.
Example 5
A formula of bio-enzyme strains in a bio-enzyme pulping process comprises the following components in parts by weight: 9 percent of bacillus, 17 percent of bifidobacterium, 21 percent of lactic acid bacteria, 13 percent of saccharomycetes, 15 percent of trichoderma reesei and 25 percent of aspergillus niger complex enzyme preparation, wherein the viable bacteria content of the microbial inoculum is more than or equal to 8 x 109cfu/g. The preparation method is the same as that of the strain in example 1.
Example 6
A formula of biological enzyme in a biological enzyme pulping process comprises the following components in parts by weight: 10% of cellulase, 12% of laccase, 12% of protease, 14% of high-temperature amylase, 10% of lignin degrading enzyme, 17% of phytase, 15% of glucanase and 10% of pectinase. The preparation method is the same as that of the biological enzyme in example 1.
Example 7
A formula of bio-enzyme strains in a bio-enzyme pulping process comprises the following components in parts by weight: 19% of bacillus, 27% of bifidobacterium, 31% of lactic acid bacteria and 23% of microzyme, wherein the viable count content of the microbial inoculum is more than or equal to 8 x 109cfu/g. The preparation method is the same as that of the strain in example 1.
And (3) performance testing:
the raw materials are selected from wheat straw, rice straw, corn stalk, bagasse, reed, cotton stalk, poplar and other fiber-containing plants, the raw materials are firstly sent into a straw cutter by a belt conveyor to be chopped, then impurity removal is carried out by a double-cone dust remover, and then the raw materials are sent into a pre-dipping tank by the belt conveyor and a pre-material conveyor, and then the same amount of bio-enzyme strains prepared in the embodiment are added for processing.
Figure 138287DEST_PATH_IMAGE002

Claims (5)

1. A bio-enzyme strain double-effect additive in a bio-enzyme pulping process is composed of fungus components and enzyme components, and is characterized by being prepared from the following components: 1-5.5% of bacillus, 3-10% of bifidobacterium, 5-15% of lactic acid bacteria, 5-8% of yeast, 6-8% of trichoderma reesei, 10-16% of an a-niger complex enzyme preparation, 3-6% of cellulase, 6-8% of laccase, 4-7% of protease, 5-8% of high-temperature amylase, 2-5% of lignin-degrading enzyme, 6-12% of phytase, 6-12% of glucanase, 3-5% of pectinase, 0.1-0.2% of EDTA, 0.1-0.5% of vitamin C, 0.01-0.1% of glycine, 0.2-0.3% of cysteine, 1-1.5% of sodium chloride, the active bacterial content of which is more than 8 x 109cfu/g, cellulase 1000U/ml, laccase 900U/ml-1200U/ml, protease 80000U/ml, high temperature amylase 40000U/mlml, 1000U/ml lignin degrading enzyme, 1000-1500U/ml phytase, 5000U/ml glucanase and 800-1200U/ml pectinase;
the aspergillus niger compound enzyme preparation is prepared by the following preparation method, taking a sterilized solid fermentation culture medium, cooling to 40-45 ℃ in a fermentation workshop subjected to disinfection treatment, diluting and uniformly mixing cultured aspergillus niger liquid seeds with distilled water, uniformly spraying the diluted liquid seeds onto the solid fermentation culture medium, uniformly mixing the fermentation culture medium, flatly spreading the mixture on a fermentation bed, wherein the thickness of the mixture is 3-5 cm, the temperature of the mixture is 28-32 ℃, and the relative humidity of the mixture is 80-90%; turning over the bed once every 12 hours, and properly spraying distilled water; co-culturing for 48 hours; and then, air-drying the fermentation material to obtain the aspergillus niger complex enzyme preparation.
2. The bio-enzyme strain double-effect additive in the bio-enzyme pulping process according to claim 1, which is characterized by being prepared from the following components: 3.5% of bacillus, 8% of bifidobacterium, 10% of lactic acid bacteria, 6% of saccharomycetes, 7% of trichoderma reesei, 12% of aspergillus niger complex enzyme preparation, 5% of cellulase, 6.5% of laccase, 6% of protease, 8% of high-temperature amylase, 4.95% of lignin degrading enzyme, 7% of phytase, 9% of glucanase, 5% of pectinase, 0.2% of EDTA, 0.3% of vitamin C, 0.05% of glycine, 0.3% of cysteine and 1.2% of sodium chloride, wherein the viable count content of the microbial inoculum is more than or equal to 8-109cfu/g。
3. A preparation method of a bio-enzyme strain double-effect additive in a bio-enzyme pulping process as claimed in claim 1, which is characterized in that a live bacteria preparation and a complex enzyme preparation are compounded according to the proportion to prepare a double-effect additive product;
respectively inoculating bacillus, bifidobacterium, lactic acid bacteria, saccharomycetes, trichoderma reesei and aspergillus niger complex enzyme preparations into wheat bran and bean-cypress enzymatic saccharification liquid, automatically fermenting 10L seeds, canning 5L of a seed culture medium, culturing at 28-32 ℃ for 20 hours according to 5% of inoculation amount, respectively drying fermentation liquor at low temperature of 40-50 ℃, adding 1% of oligosaccharide into the bacillus, bifidobacterium, lactic acid bacteria, saccharomycetes, trichoderma reesei and aspergillus niger fermentation liquor, respectively drying at low temperature of 40-50 ℃ to prepare the viable bacteria preparation;
the compound enzyme preparation is prepared by spraying liquid seeds of cellulase, laccase, protease, high temperature amylase, lignin degrading enzyme, phytase, glucanase and pectinase on wheat bran 60%, bean-tree cypress 30% and KH 0.4%2PO4In the solid culture medium, the fermentation culture medium is uniformly stirred and is flatly paved on a fermentation bed, the thickness is 3-5 cm, the temperature is 28-32 ℃, and the relative humidity is 80-90%; the bed is turned over once every 12 hours, and distilled water can be properly sprayed; co-culturing for 48 hours; then the fermentation material is dried in the air, and an enzyme preparation compounded by cellulase, laccase, protease, high-temperature amylase, lignin degrading enzyme, phytase, glucanase and pectinase is obtained.
4. The method for preparing the bio-enzyme strain double-effect additive in the bio-enzyme pulping process according to claim 3, wherein the mass ratio of bran to water in a mixture of bran and water used for fermentation in the fermentation tank is 2:3, and the bran has a particle size of 2mm to 8 mm; or the mass ratio of wheat bran to water in the mixture of wheat bran and water used for fermentation in the fermentation tank is 1:2, and the wheat bran powder has a particle size of 4mm-5 mm.
5. A preparation method of the bio-enzyme strain double-effect additive in the bio-enzyme pulping process as claimed in claim 4, characterized in that the live bacteria preparation and the complex enzyme preparation are compounded, and simultaneously, a buffer solution is added to prepare the double-effect additive.
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