CN114837007B - Method for pulping wheat straw by using composite microbial inoculum - Google Patents
Method for pulping wheat straw by using composite microbial inoculum Download PDFInfo
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- CN114837007B CN114837007B CN202210616315.3A CN202210616315A CN114837007B CN 114837007 B CN114837007 B CN 114837007B CN 202210616315 A CN202210616315 A CN 202210616315A CN 114837007 B CN114837007 B CN 114837007B
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- 239000010902 straw Substances 0.000 title claims abstract description 52
- 241000209140 Triticum Species 0.000 title claims abstract description 48
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 48
- 238000004537 pulping Methods 0.000 title claims abstract description 37
- 239000002131 composite material Substances 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000002068 microbial inoculum Substances 0.000 title claims description 17
- 230000000813 microbial effect Effects 0.000 claims abstract description 11
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 8
- 238000004519 manufacturing process Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 230000000694 effects Effects 0.000 claims description 18
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 229940088598 enzyme Drugs 0.000 claims description 9
- 238000000227 grinding Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 238000002791 soaking Methods 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 7
- 241000228245 Aspergillus niger Species 0.000 claims description 5
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 5
- 244000063299 Bacillus subtilis Species 0.000 claims description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 5
- 241001074903 Methanobacteria Species 0.000 claims description 5
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 2
- 108010059820 Polygalacturonase Proteins 0.000 claims 1
- 241001148470 aerobic bacillus Species 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 108010093305 exopolygalacturonase Proteins 0.000 claims 1
- 235000019991 rice wine Nutrition 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 6
- 238000005265 energy consumption Methods 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 229920001131 Pulp (paper) Polymers 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
- 241000233866 Fungi Species 0.000 description 9
- 229920005610 lignin Polymers 0.000 description 7
- 238000010009 beating Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 239000002994 raw material Substances 0.000 description 6
- 206010042674 Swelling Diseases 0.000 description 5
- 238000012258 culturing Methods 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 239000002002 slurry Substances 0.000 description 5
- 230000008961 swelling Effects 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 238000010411 cooking Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 241000193403 Clostridium Species 0.000 description 2
- 108010029541 Laccase Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000007670 refining Methods 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- BHNHHSOHWZKFOX-UHFFFAOYSA-N 2-methyl-1H-indole Chemical compound C1=CC=C2NC(C)=CC2=C1 BHNHHSOHWZKFOX-UHFFFAOYSA-N 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 108010054320 Lignin peroxidase Proteins 0.000 description 1
- 108010059896 Manganese peroxidase Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
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- 230000001590 oxidative effect Effects 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 125000003396 thiol group Chemical class [H]S* 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H11/00—Pulp or paper, comprising cellulose or lignocellulose fibres of natural origin only
- D21H11/12—Pulp from non-woody plants or crops, e.g. cotton, flax, straw, bagasse
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/64—Paper recycling
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Paper (AREA)
Abstract
The invention belongs to the technical field of pulping, and relates to a method for pulping wheat straw by using a composite microbial agent. The method of the invention does not use any chemicals in the production process, has no pollution, saves water, has small material loss and low energy consumption. The produced paper pulp has good quality, no chemical toxin, can be used for manufacturing corrugated paper, and has no pollution in the production process. Has very high economic benefit and social benefit, and is a high-tech high-technology value-increasing technology with ecological balance.
Description
Technical Field
The invention belongs to the technical field of pulping, and relates to a novel pulping mode for pulping wheat straws at normal temperature and normal pressure by using a composite microbial inoculum.
Background
The disclosure of this background section is only intended to increase the understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art.
With the increasing development of economy, the papermaking industry is increasingly important. With the advancement of sustainable ideas, the scientific technology in the paper industry has new directions and goals. In the future development of papermaking engineering technology, energy conservation and emission reduction and biomass refining technology are still used as handrails for transformation and upgrading and development emphasis.
The pulping and papermaking raw materials are not separated from straw fibers, mainly crop straws such as wheat straws and the like. With the improvement of crop yield, the crop straw yield is also improved, and the phenomena of straw burning and discarding are particularly serious. However, forest resources are scarce, pulping raw materials are short, and dependence on imported fiber raw materials is high, so that in order to meet the requirements of fiber raw materials at the present stage, the structure of papermaking raw materials must be quickened and adjusted, the existing grass resources are effectively and reasonably utilized, the pulping yield is improved, and a clean production technology is explored.
At present, the most used strain in paper making is white rot fungi. White rot fungi are a kind of filamentous fungi in nature, belonging to basidiomycetes, and are named because they cause wood to rot and appear white. Currently, white rot fungi have the strongest lignin degradation force in all fungi, and degrade lignin through the action of secreted enzymes. Lignin peroxidase, which depends on manganese peroxidase and laccase, is the main type of lignin-degrading enzyme secreted by white rot fungi. The white rot fungi have simple growth conditions, low culture cost and high survival rate; hydroxyl radicals formed during growth of white rot fungi have strong oxidizing property, can cause threat to growth of other species, and have competitive advantages; laccase secreted by white rot fungi can degrade various pollutants, and can thoroughly oxidize and degrade lignin into CO 2 And H 2 O shows unique superiority in lignin degradation, and is beneficial to the carbon circulation in nature. The inventors found that: the effect of the strain on the pulping treatment of the wheat straw is still to be improved.
Disclosure of Invention
In order to solve the problems, the invention provides a method for pulping wheat straw by using a composite microbial inoculant. The wheat straw is pretreated by using a composite microbial inoculum (bacillus amyloliquefaciens, bacillus subtilis, methane bacillus and aspergillus niger), lignin in the wheat straw is removed to a certain extent by utilizing the degradation effect of the wheat straw, the aim of reducing the energy consumption in the mechanical pulping process is fulfilled, the chemical dosage in the steaming pretreatment is avoided, the steaming pretreatment time is shortened, the paper forming intensity of the wheat straw pulp is improved, the pulping cost is reduced, and the pollution load of waste liquid is reduced.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
in a first aspect of the present invention, there is provided a method for pulping wheat straw using a composite microbial inoculant, comprising:
soaking wheat straw in hot water and extruding the wheat straw by a pair of rollers;
spraying a composite microbial inoculum on the wheat straw after being extruded by the pair roller for fermentation culture;
washing, high-concentration grinding, pulp sieving and PFI grinding after the culture is completed.
The invention uses the composite microbial inoculum to pretreat the wheat straw, and uses the degradation effect to remove lignin in the wheat straw to a certain extent, thereby achieving the purpose of reducing the energy consumption in the mechanical pulping process, reducing the chemical dosage in the cooking pretreatment, shortening the cooking pretreatment time, improving the paper-forming strength of the wheat straw pulp, reducing the pulping cost and reducing the pollution load of waste liquid.
In a second aspect of the invention, there is provided pulp produced by the above method, which pulp is useful in the manufacture of corrugated paper.
The invention has the beneficial effects that:
(1) The invention has important significance for recycling biomass resources, and the composite microbial inoculum is used for treatment, so that the dosage of chemical reagents is reduced, and the pulping energy consumption is reduced; meanwhile, the cooking process of the raw materials is eliminated, and the target beating degree can be obtained under the conditions of normal temperature and normal pressure.
(2) According to the requirements of wheat straw component separation and according to microbiology and metabonomics theory, the system of the invention preferably knows bacillus amyloliquefaciens, bacillus subtilis, heat-resistant clostridium and aspergillus niger, and through pure culture and solid mixed fermentation culture, accumulated active functional microorganisms and hydrolytic enzymes play roles in separating cellulose, lignin and hemicellulose in the pretreatment process of wheat straw, and simultaneously the growth of the strains inhibits the decomposition of protein and amino acid by clostridium in wheat straw to generate H 2 S, thiol, methylindole and other putrefactive products with malodor.
(3) The method has the advantages of simplicity, low cost, universality and easiness in large-scale production.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
A method for pulping wheat straw by using a composite microbial agent comprises the following steps:
(1) Soaking the recovered wheat straw according to the liquid ratio of 1:10 at 55 ℃ for swelling treatment;
(2) Extruding the soaked wheat straw into 2-3cm by a pair roller;
(3) Uniformly spraying the composite microbial inoculum on the sample;
(4) Sealing and culturing for 7-15 days at 55-60 ℃;
(5) Washing to remove hypha;
(6) High-concentration grinding and pulping, 3000rpm, a section: 0.5mm, two sections: 0.15mm. And (5) screening slurry.
(7) And (3) pulping by PFI, so that the beating degree is 40+/-3 DEG SR, and papermaking.
The above is only a preliminary determination of the process path, and the parameters in the process flow may be adjusted appropriately.
In some embodiments, the hot water soak conditions are: at 55-60 deg.c, the liquid ratio is 1 to 8-10.
In some embodiments, the soaked wheat straw is extruded to 2-3cm by a pair of rollers.
In some embodiments, the composite microbial inoculant consists of bacillus amyloliquefaciens, bacillus subtilis, methanobacteria, and aspergillus niger. The total bacterial count (cfu/mL) of the bacillus eutrophus is more than or equal to 5.0X10 9 The total number of mould cells (cfu/g) is more than or equal to 2.0X10 10 The total number of methanobacteria (cfu/mL) is more than or equal to 5.0X10 7 . Enzyme production: the protease activity (U/g) is more than or equal to 150, the saccharifying enzyme activity (U/g) is more than or equal to 1800, the cellulase activity (U/g) is more than or equal to 300, the xylanase activity (U/g) is more than or equal to 600, and the pectase activity (U/g) is more than or equal to 80.
In some embodiments, the amount of the composite microbial agent is 1-2% of the absolute dry weight of the wheat straw.
In some embodiments, the fermentation culture conditions are 55-60℃and sealed for 7-15 days.
In some embodiments, the conditions for high consistency refining are 3000 to 3500rpm, one stage: 0.5mm, two sections: 0.15mm.
In some embodiments, the PFI refiner is such that the freeness is 40±3° SR.
The invention will now be described in further detail with reference to the following specific examples, which should be construed as illustrative rather than limiting.
In the following examples, the composite microbial inoculum consisted of bacillus amyloliquefaciens, bacillus subtilis, methanobacteria, and aspergillus niger. The total bacterial count (cfu/mL) of the bacillus eutrophus is more than or equal to 5.0X10 9 The total number of mould cells (cfu/g) is more than or equal to 2.0X10 10 The total number of methanobacteria (cfu/mL) is more than or equal to 5.0X10 7 . Enzyme production: the protease activity (U/g) is more than or equal to 150, the saccharifying enzyme activity (U/g) is more than or equal to 1800, the cellulase activity (U/g) is more than or equal to 300, the xylanase activity (U/g) is more than or equal to 600, and the pectase activity (U/g) is more than or equal to 80.
Example 1:
(1) Soaking the recovered wheat straw according to the liquid ratio of 1:10 at 55 ℃ for swelling treatment;
(2) Extruding the soaked wheat straw into 2-3cm by a pair roller;
(3) 1% of the composite microbial inoculum (relative to the wheat straw) is uniformly sprayed on the sample;
(4) Sealing and culturing for 10 days at 55-60 ℃;
(5) Washing to remove hypha;
(6) High-concentration grinding and pulping, 3000rpm, a section: 0.5mm, two sections: 0.15mm. And (5) screening slurry.
(7) And (3) pulping by PFI, so that the beating degree is 40+/-3 DEG SR, and papermaking.
Example 2:
(1) Soaking the recovered wheat straw according to the liquid ratio of 1:10 at 55 ℃ for swelling treatment;
(2) Extruding the soaked wheat straw into 2-3cm by a pair roller;
(3) 2% of the composite microbial inoculum (relative to the wheat straw) is uniformly sprayed on the sample;
(4) Sealing and culturing for 10 days at 55-60 ℃;
(5) Washing to remove hypha;
(6) High-concentration grinding and pulping, 3000rpm, a section: 0.5mm, two sections: 0.15mm. And (5) screening slurry.
(7) And (3) pulping by PFI, so that the beating degree is 40+/-3 DEG SR, and papermaking.
Example 3:
(1) Soaking the recovered wheat straw according to the liquid ratio of 1:10 at 55 ℃ for swelling treatment;
(2) Extruding the soaked wheat straw into 2-3cm by a pair roller;
(3) 3% of the composite microbial inoculum (relative to the wheat straw) is uniformly sprayed on the sample;
(4) Sealing and culturing for 10 days at 55-60 ℃;
(5) Washing to remove hypha;
(6) High-concentration grinding and pulping, 3000rpm, a section: 0.5mm, two sections: 0.15mm. And (5) screening slurry.
(7) And (3) pulping by PFI, so that the beating degree is 40+/-3 DEG SR, and papermaking.
Example 4:
(1) Soaking the recovered wheat straw according to the liquid ratio of 1:10 at 55 ℃ for swelling treatment;
(2) Extruding the soaked wheat straw into 2-3cm by a pair roller;
(3) 4% of the composite microbial inoculum (relative to the wheat straw) is uniformly sprayed on the sample;
(4) Sealing and culturing for 10 days at 55-60 ℃;
(5) Washing to remove hypha;
(6) High-concentration grinding and pulping, 3000rpm, a section: 0.5mm, two sections: 0.15mm. And (5) screening slurry.
(7) And (3) pulping by PFI, so that the beating degree is 40+/-3 DEG SR, and papermaking.
The test results are shown in table 1:
TABLE 1
Therefore, the composite microbial inoculum treatment not only reduces pulping energy consumption; meanwhile, the mechanical property of the paper is obviously enhanced.
Finally, it should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and the present invention is not limited to the above-mentioned embodiments, but may be modified or substituted for some of them by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A method for pulping wheat straw by using a composite microbial agent, which is characterized by comprising the following steps:
soaking wheat straw in hot water and extruding the wheat straw by a pair of rollers;
spraying a composite microbial inoculum on the wheat straw after being extruded by the pair roller for fermentation culture;
washing, high-concentration grinding, pulp sieving and PFI grinding after the culture is completed, and obtaining the compound rice wine;
the composite microbial inoculum consists of bacillus amyloliquefaciens, bacillus subtilis, methane bacillus and aspergillus niger;
the total bacterial count of the aerobic bacillus is more than or equal to 5.0x10 9 cfu/mL, the total number of mould cells is more than or equal to 2.0X10 10 cfu/g, the total number of methanobacteria is more than or equal to 5.0X10 7 cfu/mL; enzyme production: the protease activity is more than or equal to 150U/g, the saccharifying enzyme activity is more than or equal to 1800U/g, the cellulase activity is more than or equal to 300U/g, the xylanase activity is more than or equal to 600U/g, and the pectinase activity is more than or equal to 80U/g;
the dosage of the composite microbial inoculum is 2% of the absolute dry weight of the wheat straw;
the fermentation culture condition is 55-60 ℃, and the sealed culture is carried out for 7-15 days.
2. The method for pulping wheat straw by using the composite microbial agent according to claim 1, wherein the hot water soaking conditions are as follows: at 55-60 ℃, the liquid ratio is 1:8-10.
3. The method for pulping wheat straw using the composite microbial agent according to claim 1, wherein the soaked wheat straw is extruded to 2-3cm by a pair of rolls.
4. The method for pulping wheat straw by using the composite microbial agent as claimed in claim 1, wherein the conditions of high-concentration pulping are 3000-3500 rpm, and the method comprises the following steps: 0.5mm, two sections: 0.15mm.
5. The method for pulping wheat straw using the composite microbial agent according to claim 1, wherein PFI is used for pulping to a freeness of 40±3° SR.
6. Pulp produced by the method of any one of claims 1-5.
7. The pulp of claim 6, wherein the pulp is used to make corrugated paper.
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| CN115491915B (en) * | 2022-10-28 | 2023-09-26 | 齐鲁工业大学 | Method for pulping wheat straw by utilizing composite microbial inoculum and enzyme preparation and product |
| CN115652675A (en) * | 2022-10-31 | 2023-01-31 | 齐鲁工业大学 | Bio-mechanical pulping method using compound microorganism and product |
| CN116622781A (en) * | 2023-06-13 | 2023-08-22 | 河北沼阳环境科技有限公司 | Method for producing biogas and biological fiber slurry by anaerobic fermentation of crop straw |
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| CN114837007A (en) | 2022-08-02 |
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