CN114837007A - Method for pulping wheat straw by using composite microbial inoculum - Google Patents
Method for pulping wheat straw by using composite microbial inoculum Download PDFInfo
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- CN114837007A CN114837007A CN202210616315.3A CN202210616315A CN114837007A CN 114837007 A CN114837007 A CN 114837007A CN 202210616315 A CN202210616315 A CN 202210616315A CN 114837007 A CN114837007 A CN 114837007A
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- pulping
- microbial inoculum
- composite microbial
- wheat straw
- wheat
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H11/00—Pulp or paper, comprising cellulose or lignocellulose fibres of natural origin only
- D21H11/12—Pulp from non-woody plants or crops, e.g. cotton, flax, straw, bagasse
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/64—Paper recycling
Abstract
The invention belongs to the technical field of pulping, and relates to a method for pulping wheat straws by using a composite microbial inoculum, which combines the treatment of the composite microbial inoculum with mechanical processing, sprays the crushed raw materials with the composite microbial inoculum for biological decomposition, and keeps the temperature at 55 ℃ and the decomposition time at 7-15 days. The method does not use any chemicals in the production process, has no pollution, saves water, and has small material loss and low energy consumption. The produced paper pulp has excellent quality, no chemical toxin, can be used for manufacturing corrugated paper, and has no pollution in the production process. Has high economic benefit and social benefit, and is a high-tech high-technology upgrading technology with ecological balance.
Description
Technical Field
The invention belongs to the technical field of pulping, and relates to a novel pulping mode for wheat straw pulping at normal temperature and normal pressure by using a composite microbial inoculum.
Background
The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
With the increasing development of economy, the paper making industry takes an increasingly important position. With the advance of the concept of sustainable development, the scientific technology in the field of paper industry has new direction and target. In the future development of papermaking engineering science and technology, energy conservation, emission reduction and biomass refining technologies are still used as the armrests and development focuses for transformation and upgrading.
The pulping and papermaking raw materials can not leave grass fibers, and mainly comprise crop straws such as wheat straws and the like. Along with the increase of the crop yield, the yield of crop straws is also increased, and the phenomena of straw burning and discarding are particularly serious. But forest resources are scarce, pulping raw materials are in shortage, and the dependence on imported fiber raw materials is large, so that the adjustment of the structure of the papermaking raw materials is required to be accelerated to meet the requirements of the fiber raw materials at the present stage, the existing grass resources are utilized efficiently and reasonably, the pulping yield is improved, and a clean production technology is explored, which is particularly urgent.
At present, the most used species in paper making are white rot fungi. White rot fungi are a filamentous fungus in nature, belong to basidiomycetes, and are named because they can cause wood to rot and turn white. At present, the white rot fungi have the strongest degradation force on lignin among all fungi, and degrade the lignin by the action of secreted enzymes. Lignin peroxidases, manganese-dependent peroxidases and laccases, are the main types of lignin-degrading enzymes secreted by white rot fungi. The white rot fungi have simple growth conditions, low culture cost and high survival rate; the hydroxyl free radical formed during the growth of white rot fungi has strong oxidizing property, can threaten the growth of other species, and has competitive effectCompetition for advantages; laccase secreted by white rot fungi can degrade various pollutants and can completely oxidize and degrade lignin into CO 2 And H 2 O, shows unique superiority in lignin degradation, and is beneficial to carbon cycle in nature. However, the inventor finds that: the effect of the strain on the wheat straw pulping treatment still needs to be improved.
Disclosure of Invention
In order to solve the problems, the invention provides a method for pulping wheat straws by using a compound microbial inoculum. The wheat straw is pretreated by using the composite microbial inoculum (bacillus amyloliquefaciens, bacillus subtilis, methanobacterium and aspergillus niger) and lignin in the wheat straw is removed to a certain extent by using the degradation effect of the composite microbial inoculum, so that the aim of reducing the energy consumption in the mechanical pulping process is fulfilled, the chemical consumption in the cooking pretreatment is avoided, the cooking pretreatment time is shortened, the paper-making strength of wheat straw pulp is improved, the pulping cost is reduced, and the pollution load of waste liquid is reduced.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
the invention provides a method for pulping wheat straws by using a composite microbial inoculum, which comprises the following steps:
soaking wheat straw in hot water, and extruding with rollers;
spraying a composite microbial inoculum on the wheat straws extruded by the rollers for fermentation culture;
and after the culture is finished, washing, grinding the mixture into thick liquid by a high-concentration mill, screening the thick liquid, and grinding the thick liquid by PFI to obtain the fertilizer.
The invention uses the compound microbial inoculum to pretreat the wheat straw, and utilizes the degradation effect of the compound microbial inoculum to carry out certain removal on lignin in the wheat straw, thereby achieving the purpose of reducing the energy consumption in the mechanical pulping process, reducing the chemical consumption in the cooking pretreatment, shortening the cooking pretreatment time, improving the paper-making strength of the wheat straw pulp, reducing the pulping cost and reducing the pollution load of waste liquid.
In a second aspect of the invention, there is provided a pulp prepared by the above method, which pulp is useful for making corrugated paper.
The invention has the beneficial effects that:
(1) the invention has important significance for recycling of biomass resources, and the composite microbial inoculum is utilized for treatment, so that the dosage of chemical reagents is reduced, and the pulping energy consumption is reduced; meanwhile, the cooking process of the raw materials is cancelled, and the target beating degree can be obtained under the conditions of normal temperature and normal pressure.
(2) According to the separation requirement of wheat straw components and the theory of microbiology and metabonomics, the system preferably knows bacillus amyloliquefaciens, bacillus subtilis, heat-resistant clostridium and aspergillus niger, and has the functions of separating cellulose, lignin and hemicellulose in the wheat straw pretreatment process by pure culture and solid mixed fermentation culture of accumulated active functional microorganisms and hydrolytic enzymes, and simultaneously the growth of the strains inhibits the clostridium putrescens in the wheat straw from decomposing protein and amino acid to generate H 2 S, mercaptan, methylindole and other putrefactive products with foul smell.
(3) The method has the advantages of simple operation method, low cost, universality and easy large-scale production.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
A method for pulping wheat straw by using a composite microbial inoculum comprises the following steps:
(1) soaking the recovered wheat straws at a liquid ratio of 1: 10 at 55 ℃ for swelling treatment;
(2) extruding the soaked wheat straws to 2-3 cm by using a roller;
(3) uniformly spraying the composite microbial inoculum on a sample;
(4) culturing at 55-60 deg.C under sealed condition for 7-15 days;
(5) washing to remove hyphae;
(6) high consistency mill refining, 3000rpm, one stage: 0.5mm, two stages: 0.15 mm. And (5) screening the slurry.
(7) PFI pulp grinding is carried out, the beating degree is controlled to be 40 +/-3 degrees SR, and paper making is carried out.
The above is only a preliminary determination of the process path, and the parameters in the process flow can be adjusted appropriately.
In some embodiments, the conditions of the hot water soak are: the liquid ratio is 1: 8-10 at 55-60 ℃.
In some embodiments, the soaked wheat straw is extruded to 2-3 cm by roll.
In some embodiments, the complex microbial inoculum consists of bacillus amyloliquefaciens, bacillus subtilis, methanobacterium and aspergillus niger. The total number of bacteria (cfu/mL) of the bacillus eutrophus is more than or equal to 5.0 multiplied by 10 9 The total number of mould cells (cfu/g) is more than or equal to 2.0 multiplied by 10 10 The total number of methanobacteria (cfu/mL) is not less than 5.0 × 10 7 . Producing enzyme: the protease activity (U/g) is more than or equal to 150, the saccharifying enzyme activity (U/g) is more than or equal to 1800, the cellulase activity (U/g) is more than or equal to 300, the xylanase activity (U/g) is more than or equal to 600, and the pectinase activity (U/g) is more than or equal to 80.
In some embodiments, the amount of the composite microbial inoculum is 1-2% of the absolute dry weight of the wheat straws.
In some embodiments, the fermentation culture conditions are 55-60 ℃ and the sealed culture is carried out for 7-15 days.
In some embodiments, the high consistency mill refining conditions are 3000-3500 rpm, one stage: 0.5mm, two stages: 0.15 mm.
In some embodiments, PFI refining results in a freeness of 40 ± 3 ° SR.
The present invention is described in further detail below with reference to specific examples, which are intended to be illustrative of the invention and not limiting.
In the following examples, the complex microbial inoculum consists of bacillus amyloliquefaciens, bacillus subtilis, methanobacterium and aspergillus niger. The total number of bacteria (cfu/mL) of the bacillus eutrophus is more than or equal to 5.0 multiplied by 10 9 The total number of mould cells (cfu/g) is more than or equal to 2.0 × 10 10 The total number of methanobacteria (cfu/mL) is not less than 5.0 × 10 7 . Producing enzyme: the protease activity (U/g) is more than or equal to 150, the saccharifying enzyme activity (U/g) is more than or equal to 1800, the cellulase activity (U/g) is more than or equal to 300, the xylanase activity (U/g) is more than or equal to 600, and the pectinase activity (U/g) is more than or equal to 80.
Example 1:
(1) soaking the recovered wheat straws at a liquid ratio of 1: 10 at 55 ℃ for swelling treatment;
(2) extruding the soaked wheat straws to 2-3 cm by using a roller;
(3) 1% of composite microbial inoculum (relative to wheat straws) is uniformly sprayed on a sample;
(4) culturing at 55-60 deg.C under sealed condition for 10 days;
(5) washing to remove hyphae;
(6) high consistency grinding, 3000rpm, first stage: 0.5mm, two stages: 0.15 mm. And (5) screening the slurry.
(7) PFI pulp grinding is carried out, the beating degree is controlled to be 40 +/-3 degrees SR, and paper making is carried out.
Example 2:
(1) soaking the recovered wheat straws at a liquid ratio of 1: 10 at 55 ℃ for swelling treatment;
(2) extruding the soaked wheat straws to 2-3 cm by using a roller;
(3)2 percent of complex microbial inoculum (relative to wheat straws) is uniformly sprayed on a sample;
(4) culturing at 55-60 deg.C under sealed condition for 10 days;
(5) washing to remove hyphae;
(6) high consistency grinding, 3000rpm, first stage: 0.5mm, two stages: 0.15 mm. And (5) screening the slurry.
(7) PFI pulp grinding is carried out, the beating degree is controlled to be 40 +/-3 degrees SR, and paper making is carried out.
Example 3:
(1) soaking the recovered wheat straws at a liquid ratio of 1: 10 at 55 ℃ for swelling treatment;
(2) extruding the soaked wheat straws to 2-3 cm by using a roller;
(3)3 percent of complex microbial inoculum (relative to wheat straws) is uniformly sprayed on the sample;
(4) culturing at 55-60 deg.C under sealed condition for 10 days;
(5) washing to remove hyphae;
(6) high consistency mill refining, 3000rpm, one stage: 0.5mm, two stages: 0.15 mm. And (5) screening the slurry.
(7) PFI pulp grinding is carried out, the beating degree is controlled to be 40 +/-3 degrees SR, and paper making is carried out.
Example 4:
(1) soaking the recovered wheat straws at a liquid ratio of 1: 10 at 55 ℃ for swelling treatment;
(2) extruding the soaked wheat straws to 2-3 cm by using a roller;
(3) 4% of complex microbial inoculum (relative to wheat straws) is uniformly sprayed on the sample;
(4) culturing at 55-60 deg.C under sealed condition for 10 days;
(5) washing to remove hyphae;
(6) high consistency grinding, 3000rpm, first stage: 0.5mm, two stages: 0.15 mm. And (5) screening the slurry.
(7) PFI pulp grinding is carried out, the beating degree is controlled to be 40 +/-3 degrees SR, and paper making is carried out.
The test results are shown in table 1:
TABLE 1
Therefore, the composite microbial inoculum treatment of the invention not only reduces the pulping energy consumption; meanwhile, the mechanical property of the paper is obviously enhanced.
It should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and the present invention is not limited thereto, and although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and equivalents can be made in the technical solutions described in the foregoing embodiments, or equivalents thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A method for pulping wheat straws by using a composite microbial inoculum is characterized by comprising the following steps:
soaking wheat straw in hot water, and extruding with rollers;
spraying a composite microbial inoculum on the wheat straws extruded by the rollers for fermentation culture;
and after the culture is finished, washing, grinding the mixture into thick liquid by a high-concentration mill, screening the thick liquid, and grinding the thick liquid by PFI to obtain the fertilizer.
2. The method for wheat straw pulping by using the composite microbial inoculum according to claim 1, wherein the hot water soaking condition is as follows: the liquid ratio is 1: 8-10 at 55-60 ℃.
3. The method for pulping by using the composite microbial inoculant according to claim 1, wherein the soaked wheat straw is extruded to be 2-3 cm by rolls.
4. The method for wheat straw pulping by using the composite microbial inoculum according to claim 1, wherein the composite microbial inoculum comprises bacillus amyloliquefaciens, bacillus subtilis, methanobacterium and aspergillus niger.
5. The method for pulping wheat straw by using the composite microbial inoculum as claimed in claim 1, wherein the dosage of the composite microbial inoculum is 1-2% of the absolute dry weight of the wheat straw.
6. The method for pulping wheat straw by using the composite microbial inoculum as claimed in claim 1, wherein the fermentation culture condition is 55-60 ℃, and the sealed culture is carried out for 7-15 days.
7. The method for pulping wheat straw by using the composite microbial inoculant according to claim 1, wherein the conditions of high-consistency grinding and pulping are 3000-3500 rpm, and the steps of one section: 0.5mm, two stages: 0.15 mm.
8. The method for wheat straw pulping by using the composite microbial inoculum as claimed in claim 1, wherein PFI pulping is carried out, so that the pulping degree is within 40 +/-3 degrees SR.
9. A pulp produced by the method of any of claims 1-8.
10. A pulp according to claim 9, wherein the pulp is used to make corrugated paper.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115491915A (en) * | 2022-10-28 | 2022-12-20 | 齐鲁工业大学 | Method for pulping wheat straw by using composite microbial inoculum in cooperation with enzyme preparation and product |
| CN115652675A (en) * | 2022-10-31 | 2023-01-31 | 齐鲁工业大学 | Bio-mechanical pulping method using compound microorganism and product |
| CN116622781A (en) * | 2023-06-13 | 2023-08-22 | 河北沼阳环境科技有限公司 | Method for producing biogas and biological fiber slurry by anaerobic fermentation of crop straw |
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