CN106669873B - 一种用于细胞冷冻的微流控芯片及混合系统及其控制方法 - Google Patents
一种用于细胞冷冻的微流控芯片及混合系统及其控制方法 Download PDFInfo
- Publication number
- CN106669873B CN106669873B CN201710087443.2A CN201710087443A CN106669873B CN 106669873 B CN106669873 B CN 106669873B CN 201710087443 A CN201710087443 A CN 201710087443A CN 106669873 B CN106669873 B CN 106669873B
- Authority
- CN
- China
- Prior art keywords
- flow channel
- cell
- reagent
- channel
- inflow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002156 mixing Methods 0.000 title claims abstract description 86
- 230000008014 freezing Effects 0.000 title claims abstract description 47
- 238000007710 freezing Methods 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 171
- 239000007788 liquid Substances 0.000 claims abstract description 62
- 239000011259 mixed solution Substances 0.000 claims description 45
- 239000011521 glass Substances 0.000 claims description 11
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 9
- 230000004888 barrier function Effects 0.000 claims description 4
- 238000012544 monitoring process Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 2
- 238000011010 flushing procedure Methods 0.000 claims description 2
- 238000005057 refrigeration Methods 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 description 182
- 230000006872 improvement Effects 0.000 description 16
- 239000000203 mixture Substances 0.000 description 14
- 230000008569 process Effects 0.000 description 10
- 235000013601 eggs Nutrition 0.000 description 9
- 230000008859 change Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 239000012530 fluid Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 210000004681 ovum Anatomy 0.000 description 4
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 230000000739 chaotic effect Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 206010042573 Superovulation Diseases 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005138 cryopreservation Methods 0.000 description 2
- 239000002577 cryoprotective agent Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 239000010408 film Substances 0.000 description 2
- AMGQUBHHOARCQH-UHFFFAOYSA-N indium;oxotin Chemical compound [In].[Sn]=O AMGQUBHHOARCQH-UHFFFAOYSA-N 0.000 description 2
- 208000000509 infertility Diseases 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 231100000535 infertility Toxicity 0.000 description 2
- 238000003475 lamination Methods 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000036647 reaction Effects 0.000 description 2
- 239000010409 thin film Substances 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 239000000592 Artificial Cell Substances 0.000 description 1
- -1 Polytetrafluoroethylene Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
- B01F33/3017—Mixing chamber
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/304—Micromixers the mixing being performed in a mixing chamber where the products are brought into contact
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K7/00—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements
- G01K7/02—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements using thermoelectric elements, e.g. thermocouples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0621—Control of the sequence of chambers filled or emptied
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Dispersion Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710087443.2A CN106669873B (zh) | 2017-02-17 | 2017-02-17 | 一种用于细胞冷冻的微流控芯片及混合系统及其控制方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710087443.2A CN106669873B (zh) | 2017-02-17 | 2017-02-17 | 一种用于细胞冷冻的微流控芯片及混合系统及其控制方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106669873A CN106669873A (zh) | 2017-05-17 |
CN106669873B true CN106669873B (zh) | 2022-05-10 |
Family
ID=58862229
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710087443.2A Active CN106669873B (zh) | 2017-02-17 | 2017-02-17 | 一种用于细胞冷冻的微流控芯片及混合系统及其控制方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106669873B (zh) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107629941A (zh) * | 2017-10-27 | 2018-01-26 | 中国科学院理化技术研究所 | 一种基于微流控芯片的细胞冻存装置及其应用 |
CN108841713B (zh) * | 2018-06-12 | 2020-10-20 | 深圳韦拓生物科技有限公司 | 理化处理单个细胞的微流控芯片、微流控装置及使用其理化处理单个细胞的方法 |
CN109490058B (zh) * | 2018-11-16 | 2021-04-20 | 南京爱思唯志生物科技有限公司 | 一种适于液体混合的微流控系统及方法 |
CN112403539A (zh) * | 2019-08-23 | 2021-02-26 | 无锡源清天木生物科技有限公司 | 一种微流控芯片 |
CN112964865B (zh) * | 2021-01-18 | 2022-10-14 | 中山大学 | 一种通道形貌控制流体混合的超快速免疫检测芯片及其制备方法和应用 |
CN115777684A (zh) * | 2021-09-10 | 2023-03-14 | 深圳拜尔洛克生物技术有限公司 | 用于生物组织冷冻保存或解冻复苏的装置 |
CN114223650A (zh) * | 2021-12-29 | 2022-03-25 | 序康医疗科技(苏州)有限公司 | 一种生物材料的冷冻保存方法及装置 |
CN116445246B (zh) * | 2023-06-13 | 2023-09-08 | 广州凯普医药科技有限公司 | 一种用于活体细胞分选的微流控芯片 |
CN117920015A (zh) * | 2024-03-20 | 2024-04-26 | 上海复迪源码生物技术有限公司 | 用于制备纳米制剂的制备系统及控制方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101135680A (zh) * | 2007-07-13 | 2008-03-05 | 东南大学 | 光诱导介电泳辅助单细胞介电谱自动测试装置及测试方法 |
CN201488996U (zh) * | 2009-08-06 | 2010-05-26 | 浙江大学 | 一种检测神经细胞电信号的装置 |
CN104075977A (zh) * | 2014-07-18 | 2014-10-01 | 苏州大学 | 一种细胞吸吐测量装置 |
CN105831105A (zh) * | 2016-04-12 | 2016-08-10 | 上海理工大学 | 微流体细胞处理芯片及其应用方法 |
CN206535564U (zh) * | 2017-02-17 | 2017-10-03 | 深圳韦拓生物科技有限公司 | 一种用于细胞冷冻的微流控芯片及混合系统 |
-
2017
- 2017-02-17 CN CN201710087443.2A patent/CN106669873B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101135680A (zh) * | 2007-07-13 | 2008-03-05 | 东南大学 | 光诱导介电泳辅助单细胞介电谱自动测试装置及测试方法 |
CN201488996U (zh) * | 2009-08-06 | 2010-05-26 | 浙江大学 | 一种检测神经细胞电信号的装置 |
CN104075977A (zh) * | 2014-07-18 | 2014-10-01 | 苏州大学 | 一种细胞吸吐测量装置 |
CN105831105A (zh) * | 2016-04-12 | 2016-08-10 | 上海理工大学 | 微流体细胞处理芯片及其应用方法 |
CN206535564U (zh) * | 2017-02-17 | 2017-10-03 | 深圳韦拓生物科技有限公司 | 一种用于细胞冷冻的微流控芯片及混合系统 |
Also Published As
Publication number | Publication date |
---|---|
CN106669873A (zh) | 2017-05-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106669873B (zh) | 一种用于细胞冷冻的微流控芯片及混合系统及其控制方法 | |
US20190133114A1 (en) | Microfluidic Embryo and Gamete Culture Systems | |
US10710077B2 (en) | Method for handling microdrops which include samples | |
CN206535564U (zh) | 一种用于细胞冷冻的微流控芯片及混合系统 | |
CN105831105B (zh) | 微流体细胞处理芯片及其应用方法 | |
KR100742783B1 (ko) | 세포 분리 칩 | |
JP2004500241A (ja) | タンパク質の結晶化のマイクロ流動体装置 | |
Zhao et al. | A microfluidic perfusion approach for on-chip characterization of the transport properties of human oocytes | |
US11911763B2 (en) | Microfluidic device and methods | |
JP2019525836A (ja) | マイクロ流体デバイスにおける気泡の除去 | |
TWI579551B (zh) | 收集元件與具有該元件之樣本處理套組 | |
WO2010011774A1 (en) | Collection device and method for stimulating and stabilizing a biological sample | |
Six et al. | A microfluidic flow chamber model for platelet transfusion and hemostasis measures platelet deposition and fibrin formation in real-time | |
US20130302842A1 (en) | Lid For Functionalized Microfluidic Platform And Method | |
KR20150081207A (ko) | 세포 트랩이 가능한 미세 모세관 | |
JP2024503211A (ja) | 細胞洗浄、磁気単離および投与調整のための使い捨てキット | |
KR101619170B1 (ko) | 복수시료에 대한 세포 시료감응성의 순차적 측정을 위한 미세유체칩 | |
CN212393695U (zh) | 一种精子冷冻载体系统 | |
CN116529354A (zh) | 用于使用加压验证生物处理系统的完整性的系统和方法 | |
CN114849797A (zh) | 一种基于相变材料封闭试剂的微流控芯片 | |
CN114437932B (zh) | 多胎胎盘血管吻合模型的微流控芯片、系统及使用方法 | |
US20230416666A1 (en) | Culture vessel for a bioprocessing system | |
US20240018457A1 (en) | Methods for cell activation, transduction and expansion | |
JP2024501628A (ja) | バイオプロセッシングシステムの中の流体を混合するためのシステム、方法、および装置 | |
WO2006097740A1 (en) | Capillary devices for cell and non-human embryo culture |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Lin Xiaozhen Inventor after: Lin Xiaozhen Gabor Wajita. Inventor before: Lin Xiaozhen Inventor before: Lin Xiaozhen Gabor Wajita. |
|
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Lin Xiaozhen Inventor after: Gai Bo.wajita Inventor before: Lin Xiaozhen Inventor before: Garber Wagita |
|
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: 518000 Room 601, building B, haikexing strategic emerging industrial park, No. 16, Baoshan Road, Pingshan street, Pingshan District, Shenzhen, Guangdong Applicant after: SHENZHEN VITAVITRO BIOTECH Co.,Ltd. Address before: 518000 Room 501, building B, haikexing strategic emerging industrial park, No. 16, Baoshan Road, Pingshan street, Pingshan new area, Shenzhen, Guangdong Applicant before: SHENZHEN VITAVITRO BIOTECH Co.,Ltd. |
|
GR01 | Patent grant | ||
GR01 | Patent grant |