CN106666238A - Method for preparing feed additive for litopenaeus vannamei from salicornia europaea with enzymolysis and fermentation processes - Google Patents
Method for preparing feed additive for litopenaeus vannamei from salicornia europaea with enzymolysis and fermentation processes Download PDFInfo
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- CN106666238A CN106666238A CN201611241665.7A CN201611241665A CN106666238A CN 106666238 A CN106666238 A CN 106666238A CN 201611241665 A CN201611241665 A CN 201611241665A CN 106666238 A CN106666238 A CN 106666238A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention discloses a feed additive which is prepared from salicornia europaea with enzymolysis and fermentation processes and used for litopenaeus vannamei. The additive has multiple efficacies of prevention, health care, treatment, immunoregulation and the like and is an ideal, green, environment-friendly, safe and novel feed additive for replacing an antibiotic drug. The immunopotentiator is prepared from salicornia europaea, radix et caulis acanthopanacis senticosi, herba dendrobii, fructus hordei germinatus, spirulina, radix platycodonis, radix glycyrrhizae, radix rehmanniae preparata, rhizoma imperatae, radix gentianae and other Chinese medicinal herbs with processes including enzymatic extraction, ultrasonic wave-assisted extraction and two-step fermentation including aerobic fermentation and anaerobic fermentation by multiple strains. The feed additive has multiple advantages of salicornia europaea, Chinese medicinal herbs and probiotics, can efficiently regulate intestinal tract health of litopenaeus vannamei, treat both symptoms and root causes, reinforce the vital essence, strengthen the primordial qi, keep microcosmic ecological balance and the best immune state of intestinal tracts, increase the digestion and absorption rate of nutrients, improve the immunity of litopenaeus vannamei, increase the aquiculture yield and reduce the aquiculture risk, replaces antibiotics, has no bad influence on litopenaeus vannamei, is safe to use, does not have the problem of drug residues, drug resistance and the like and has broad market prospect.
Description
Technical field
The present invention relates to a kind of method that joint enzymatic isolation method and fermentation method prepare Salicornia Bigelovii Torr. penaeus vannamei boone feed additive,
Belong to feed additive field.
Background technology
Penaeus vannamei scientific name Litoenaeus vannamei, it is category Arthropoda, Crustachia, Decapoda, swimming suborder, Penaeidae, right
Shrimp category, Lito-penaeus subgenus, are wide temperature eurysalinity torrid zone shrimps, and average life at least can be more than 32 months.Adult is most
Long up to 24cm, carapace is relatively thin, and normal body colour is shallow cinerouss, and whole body does not have speckle.Step is often in Chalk shape, therefore has white limb shrimp
Title.Penaeus vannamei originates in America pacific rim waters, and main distribution northern Peru is littoral to the Gulf of Mexico, with melon in distress
The littoral distribution of Dorr is concentrated the most.Penaeus vannamei has the advantages that individual big, growth is fast, nutritional need is low, premunition is strong, right
The adaptability of water environment factors is stronger, it is low to forage protein content requirement, go out rate be up to more than 65%, from water survive when
Between it is long the advantages of, be the improved seeds of intensive high yielding culture, be also current single rate highest in 3 big cultured prawns in the world
Shrimp species.The thin body fertilizer of Penaeus vannamei shell, delicious meat, dressed fish is high, nutritious, great breeding prospect.
In order to meet the demand of ever-increasing aquatic products, solve because aquaculture scale constantly expands and growth cycle contracting
Subtract and caused physiological disorders, disease take place frequently and become the Important Problems of aquaculture, be also aquatic feeds industry development
It is crucial.In this context, antibioticses feed additive is subject to heat handful, in being widely used in feed industry, to a certain extent
Promote the development of culture fishery, but antibiotic bring high residue, high pollution and Drug resistance the problems such as more serious.
Develop the environment-friendly feed additives such as microbial ecological agent, Chinese herbal feed additive successively at present, but still suffer from effect
Effect does not know, be affected by the external environment the drawback such as larger, and especially for marine cultured animal, Chinese herbal feed additive is deposited
In the drawback such as palatability is poor, water pollution is serious, therefore it is beneficial to based on ocean or alkaline development of resources novel fodder additive
It is pushed further into the development of Penaeus vannamei ecologic breeding industry.
Salicornia Bigelovii Torr. belongs to jasmine section plant, Salicornia Bigelovii Torr. category, in salt life also known as Salicornia Bigelovii Torr., extra large Cornu Cervi Pantotrichum, marine borer grass, rich and honour dish etc.
In plant classification, belong to stem carnification euhalophyte, be more common in marshland, salt lake side and and seabeach.Salicornia Bigelovii Torr. its be dried plant
Strain cellulose 5~20%, protein 9.0%, rich in 8 kinds of aminoacid, trace element and vitamin needed by human, wherein recklessly
Radix Raphani cellulose content exceedes 40 times of common vegetable, also containing natural plant health salt and natural plant alkaloid (micro- angle saponin), tool
There is the effects such as reduction cholesterol, blood pressure lowering.The plantation of Salicornia Bigelovii Torr. has a significant application value to improving China's saline-alkali environment, but at present
The comprehensive development and utilization degree of Salicornia Bigelovii Torr. is relatively low, governs Salicornia Bigelovii Torr. secondary industry economic benefit, also directly affects Salicornia Bigelovii Torr.
Plantation promote, therefore, if Salicornia Bigelovii Torr. is carried out into high-valued comprehensive development and utilization, important tribute will be made to environment and economy
Offer.
The content of the invention
It is an object of the invention to according to above-mentioned deficiency present in prior art, based on the idea of development of recycling economy,
Terrestrial Soiline-alkali plants resource Salicornia Bigelovii Torr. is developed using modern biotechnology and prepares Penaeus vannamei feed additive, can be shown
Write and improve the immunity of organisms of Penaeus vannamei, improve growth of animal performance, reduce feed coefficient, reduce antibiotic and use in a large number
The aquaculture pollution for causing, and raw material sources are wide, it is cheap, it is a kind of green, environmentally friendly, efficient feed additive, develop
Salicornia Bigelovii Torr. penaeus vannamei boone feed additive prepared by a kind of joint enzymatic isolation method of exploitation and fermentation method.
Above-mentioned purpose of the present invention is achieved by the following technical programs：
It is a kind of joint enzymatic isolation method and fermentation method prepare Salicornia Bigelovii Torr. penaeus vannamei boone feed additive, it is characterised in that by with
The raw material of lower mass ratio is made：30~50 parts of Salicornia Bigelovii Torr. Powder, 15~20 parts of Herba Taraxaci, 8~12 parts of Radix Et Caulis Acanthopanacis Senticosi, 8~12 parts of Herba Dendrobii,
6~8 parts of Fructus Hordei Germinatus, 3~5 parts of spirulina, 6~8 parts of Radix Platycodoniss, 6~8 parts of Radix Glycyrrhizae, 3~5 parts of Radix Rehmanniae Preparata, 5~7 parts of Herba Imperatae, Radix Gentianae 3~5
Part.
Salicornia Bigelovii Torr. penaeus vannamei boone feed additive prepared by a kind of joint enzymatic isolation method and fermentation method, it is characterised in that described
Raw material composition is preferably：40 parts of Salicornia Bigelovii Torr. Powder, 18 parts of Herba Taraxaci, 10 parts of Radix Et Caulis Acanthopanacis Senticosi, 10 parts of Herba Dendrobii, 7 parts of Fructus Hordei Germinatus, 4 parts of spirulina,
7 parts of Radix Platycodoniss, 7 parts of Radix Glycyrrhizae, 4 parts of Radix Rehmanniae Preparata, 6 parts of Herba Imperatae, 4 parts of Radix Gentianae.
The present invention provides the technique that a kind of bioanalysises prepare penaeus vannamei boone feed additive using Salicornia Bigelovii Torr., the Hai Peng
Sub- powder pretreating process is as follows：
(1) immersion is crushed：Deimpurity Salicornia Bigelovii Torr. cane will be gone to crush, cross 100 mesh sieves, be subsequently adding 2 times of mass ratioes pure
Water purification soaks 3~5h；
(2) steaming and decocting：By the Salicornia Bigelovii Torr. suspension 1~2h of steaming and decocting after immersion, then decline in air-proof condition and warm to room temperature；
(3) separate：Cooking liquor is carried out into solid-liquid separation, Salicornia Bigelovii Torr. cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(4) become thoroughly decomposed naturally：Control Salicornia Bigelovii Torr. cane powder water content 40~50%, 30~50 DEG C of temperature control, become thoroughly decomposed 30 naturally~
50d；
(5) two-step fermentation enzymolysis：Naturally become thoroughly decomposed after end, add Salicornia Bigelovii Torr. mass ratio 2% cellulase and 1% it is non-
Starch-polysaccharides enzyme, and be inoculated with the salt tolerant Alkaliphilic bacillus of Salicornia Bigelovii Torr. mass ratio 2~3%, 28~35 DEG C of condition bottom fermentations 3~
5d, stirring in 12 hours is once；Salicornia Bigelovii Torr. yeast powder is obtained final product after fermentation ends.
The present invention provides the technique that a kind of bioanalysises prepare penaeus vannamei boone feed additive using Salicornia Bigelovii Torr., and its feature exists
In comprising the steps：
(1) co-grinding：Each raw material is mixed in proportion, 100 mesh sieves were then crushed；Add the pure of 3 times of raw materials quality
Water purification；
(2) digest：Cellulase, 2~3% non-starch polysaccharide enzyme of herbal raw material mass ratio 3~5% are added,
Temperature control digests 4~8h under the conditions of 40~60 DEG C, stirs once every 0.5h；
(3) it is separated by filtration：Enzymolysis solution is filtered, enzymolysis solution and enzymolysis residue is obtained；
(4) Ultrasonic Wave-Assisted Extraction：3~5 times of pure water is added in enzymolysis residue, under ultrasonic assistant effect,
20~30min of backflow extraction, ultrasonic power 400w, frequency 25kHz under the conditions of 100 DEG C；Ultrasonic power 400w, frequency 25kHz；
(5) it is separated by filtration：Lixiviating solution is filtered, lixiviating solution and leaching residue is obtained；
(6) prepared by fermentation liquid：The pure water of 5 times of mass ratioes is added in the leaching residue in step (5), and adds residue
The glucose of mass ratio 3.0~5.0%, 2.0~3.0% yeast extracts, 0.3~0.5% potassium dihydrogen phosphate, 0.02%MgSO4、
0.01%CaCl2, pH is adjusted to 7.0~7.2；
(7) aerobic fermentation：From liquid fermentation tank, liquid amount volume ratio is 60~70%, inoculation bacillus megaterium and
Aspergillus niger, inoculum concentration is respectively 2~4%, 1~2%, controls 28~37 DEG C of fermentation temperature, 150~300rpm of rotating speed；At this
Ferment condition 12~24h of bottom fermentation；
(8) anaerobic fermentation：After aerobic fermentation terminates, streptococcus thermophiluss and Lactobacillus bulgaricus are inoculated with, inoculum concentration is 2
~4%, 28~37 DEG C of fermentation temperature, temperature control 12~24h of anaerobic fermentation；
(9) merge：Fermentation liquid in lixiviating solution and step (8) in enzymolysis solution in step (3), step (5) is merged；
(10) cryoconcentration：The enhancing agent solution after step (9) merging is concentrated under cryogenic；
(11) adsorption dry：With the wheat bran absorption of 1~1.5 times of mass ratio after concentration, in 40~60 DEG C of blast driers
It is dried, moisture Control is 8~10%；
(12) crush：The dried mesh of product pulverizer 60 is sieved crushing, Penaeus vannamei is obtained final product and is added with feedstuff
Agent.
The present invention provides Salicornia Bigelovii Torr. penaeus vannamei boone feed additive prepared by a kind of joint enzymatic isolation method and fermentation method, has
The multiple efficacies such as prevention, health care, treatment and regulation immunity, are the preferable, green, environmentally friendly, safe, new of substitute antibiotics medicine
Feed additive.The invention feed additive one can be efficiently to nurse one's health Penaeus vannamei intestinal health, and treating both the principal and secondary aspects of a disease consolidates training
Unit, keeps intestinal microecology balance and optimal immune state, improves the digestibility of nutrient substance, improves animal body immunity
Power；Two is the generation that can suppress and prevent the harmful bacterias such as cause of disease coliform, clostridium, Salmonella, increases intestinal benefit
Raw bacterium, maintains the intestinal microbial population of health；Three is to strengthen immunization, by stimulating siberian crabapple cell in intestinal, increases local anti-
The formation of body, increases macrophage activity；Four is that feed additive enters enteral resistance harmful bacteria, reduces stench and harmful components
Formed, the feces for making discharge eliminate stench, Penaeus vannamei meat quality is improved while breeding environment is protected.
The present invention provides Salicornia Bigelovii Torr. penaeus vannamei boone feed additive prepared by a kind of joint enzymatic isolation method and fermentation method, described
Mass ratio of the Penaeus vannamei feedstuff additive product used in aquatic feeds complete feedstuff is 0.1~0.5%：Or it is described
Feed additive premix material, concentrate feed with needed for complete feedstuff ratio addition use.
The present invention provides Salicornia Bigelovii Torr. penaeus vannamei boone feed additive prepared by a kind of joint enzymatic isolation method and fermentation method, and its is excellent
Gesture is：One is the combination principle according to Chinese herbal medicine monarch, with terrestrial Soiline-alkali plants Salicornia Bigelovii Torr. as monarch drug, is aided with Pu public
English, Radix Et Caulis Acanthopanacis Senticosi, Herba Dendrobii are ministerial drug, with Fructus Hordei Germinatus, spirulina, Radix Platycodoniss, Radix Rehmanniae Preparata, Herba Imperatae, Radix Gentianae as adjuvant drug, with Radix Glycyrrhizae as medicine is made, altogether
The effect of row complements each other；Especially Salicornia Bigelovii Torr. is rich in natural plant health salt and natural plant alkaloid (micro- angle saponin), right
Marine fish is compared with Chinese traditional herbs health-care effect more preferably；Two be employ in feed additive preparation process " enzymolysis and extraction+
The production technology of Ultrasonic Wave-Assisted Extraction+multi-cultur es aerobic-anaerobic fermentation two-step fermentation ", with existing Chinese herbal feed additive
It is more notable that product compares action effect；Three is the multiple advantage that feed additive has Salicornia Bigelovii Torr., Chinese herbal medicine and probiotic bacteria etc., can
Efficiently to nurse one's health Penaeus vannamei intestinal health, treating both the principal and secondary aspects of a disease, vital base and primordial QI consolidating keeps intestinal microecology balance and optimal immunity shape
State, improves the digestibility of nutrient substance, improves animal body immunity, improves cultivation income, reduces cultivation risk；Four are
The alternative antibiotic usage of the product, has no adverse effects to Penaeus vannamei, using safety, there is no drug residue and drug resistance
The problems such as, there is wide market prospect.
Specific embodiment
Embodiment 1：
Salicornia Bigelovii Torr. penaeus vannamei boone feed additive prepared by a kind of joint enzymatic isolation method and fermentation method, concrete preparation process is such as
Under：
(1) immersion is crushed：Deimpurity Salicornia Bigelovii Torr. cane will be gone to crush, cross 100 mesh sieves, be subsequently adding 2 times of mass ratioes pure
Water purification soaks 4h；
(2) steaming and decocting：By the Salicornia Bigelovii Torr. suspension steaming and decocting 1.5h after immersion, then decline in air-proof condition and warm to room temperature；
(3) separate：Cooking liquor is carried out into solid-liquid separation, Salicornia Bigelovii Torr. cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(4) become thoroughly decomposed naturally：Control Salicornia Bigelovii Torr. cane powder water content 40~50%, 30~50 DEG C of temperature control, become thoroughly decomposed 40d naturally；
(5) two-step fermentation enzymolysis：Naturally become thoroughly decomposed after end, add Salicornia Bigelovii Torr. mass ratio 2% cellulase and 1% it is non-
Starch-polysaccharides enzyme, and the salt tolerant Alkaliphilic bacillus of Salicornia Bigelovii Torr. mass ratio 2.5% are inoculated with, in 28~35 DEG C of condition bottom fermentation 4d,
Stirring in 12 hours is once；Salicornia Bigelovii Torr. yeast powder is obtained final product after fermentation ends；
(6) co-grinding：By each raw material by extra large 40 parts of Salicornia Bigelovii Torr. Powder, 18 parts of Herba Taraxaci, 10 parts of Radix Et Caulis Acanthopanacis Senticosi, 10 parts of Herba Dendrobii,
7 parts of Fructus Hordei Germinatus, 4 parts of spirulina, 7 parts of Radix Platycodoniss, 7 parts of Radix Glycyrrhizae, 4 parts of Radix Rehmanniae Preparata, 6 parts of Herba Imperatae, 4 parts of ratio mixing of Radix Gentianae, then crushed
100 mesh sieves；Add the pure water of 3 times of raw materials quality；
(7) digest：Cellulase, 2.5% non-starch polysaccharide enzyme of herbal raw material mass ratio 4% are added, 40~
Temperature control enzymolysis 6h, stirs once every 0.5h under the conditions of 60 DEG C；
(8) it is separated by filtration：Enzymolysis solution is filtered, enzymolysis solution and enzymolysis residue is obtained；
(9) Ultrasonic Wave-Assisted Extraction：4 times of pure water, under ultrasonic assistant effect, 100 are added in enzymolysis residue
Backflow extraction 25min, ultrasonic power 400w, frequency 25kHz under the conditions of DEG C；Ultrasonic power 400w, frequency 25kHz；
(10) it is separated by filtration：Lixiviating solution is filtered, lixiviating solution and leaching residue is obtained；
(11) prepared by fermentation liquid：The pure water of 5 times of mass ratioes is added in the leaching residue in step (10), and is added residual
The glucose of slag mass ratio 4.0%, 2.5% yeast extract, 0.4% potassium dihydrogen phosphate, 0.02%MgSO4, 0.01%CaCl2, pH is adjusted to
7.0~7.2；
(12) aerobic fermentation：From liquid fermentation tank, liquid amount volume ratio is 60~70%, inoculation bacillus megaterium and
Aspergillus niger, inoculum concentration is respectively 3%, 1.5%, controls 28~37 DEG C of fermentation temperature, 150~300rpm of rotating speed；Here fermentation bar
Part bottom fermentation 18h；
(13) anaerobic fermentation：After aerobic fermentation terminates, streptococcus thermophiluss and Lactobacillus bulgaricus are inoculated with, inoculum concentration is
3%, 28~37 DEG C of fermentation temperature, temperature control anaerobic fermentation 18h；
(14) merge：Fermentation liquid in lixiviating solution and step (13) in enzymolysis solution in step (8), step (10) is closed
And；
(15) cryoconcentration：The enhancing agent solution after step (14) merging is concentrated under cryogenic；
(16) adsorption dry：With the wheat bran absorption of 1~1.5 times of mass ratio after concentration, in 40~60 DEG C of blast driers
It is dried, moisture Control is 8~10%；
(17) crush：The dried mesh of product pulverizer 60 is sieved crushing, Penaeus vannamei is obtained final product and is added with feedstuff
Agent.
Embodiment 2：
Salicornia Bigelovii Torr. penaeus vannamei boone feed additive prepared by a kind of joint enzymatic isolation method and fermentation method, concrete preparation process is such as
Under：
(1) immersion is crushed：Deimpurity Salicornia Bigelovii Torr. cane will be gone to crush, cross 100 mesh sieves, be subsequently adding 2 times of mass ratioes pure
Water purification soaks 5h；
(2) steaming and decocting：By the Salicornia Bigelovii Torr. suspension steaming and decocting 2h after immersion, then decline in air-proof condition and warm to room temperature；
(3) separate：Cooking liquor is carried out into solid-liquid separation, Salicornia Bigelovii Torr. cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(4) become thoroughly decomposed naturally：Control Salicornia Bigelovii Torr. cane powder water content 40~50%, 30~50 DEG C of temperature control, become thoroughly decomposed 30d naturally；
(5) two-step fermentation enzymolysis：Naturally become thoroughly decomposed after end, add Salicornia Bigelovii Torr. mass ratio 2% cellulase and 1% it is non-
Starch-polysaccharides enzyme, and be inoculated with the salt tolerant Alkaliphilic bacillus of Salicornia Bigelovii Torr. mass ratio 3%, in 28~35 DEG C of condition bottom fermentation 5d, 12
Hour stirring is once；Salicornia Bigelovii Torr. yeast powder is obtained final product after fermentation ends；
(6) co-grinding：Each raw material is pressed into 50 parts of Salicornia Bigelovii Torr. Powder, 15 parts of Herba Taraxaci, 12 parts of Radix Et Caulis Acanthopanacis Senticosi, 8 parts of Herba Dendrobii, Fructus Hordei Germinatus
8 parts, 3 parts of spirulina, 8 parts of Radix Platycodoniss, 6 parts of Radix Glycyrrhizae, 5 parts of Radix Rehmanniae Preparata, 5 parts of Herba Imperatae, the mixing of 5 parts of ratios of Radix Gentianae, then crushed 100 mesh
Sieve；Add the pure water of 3 times of raw materials quality；
(7) digest：Cellulase, 3% non-starch polysaccharide enzyme of herbal raw material mass ratio 5% are added, 40~60
Temperature control enzymolysis 4h, stirs once every 0.5h under the conditions of DEG C；
(8) it is separated by filtration：Enzymolysis solution is filtered, enzymolysis solution and enzymolysis residue is obtained；
(9) Ultrasonic Wave-Assisted Extraction：5 times of pure water, under ultrasonic assistant effect, 100 are added in enzymolysis residue
Backflow extraction 20min, ultrasonic power 400w, frequency 25kHz under the conditions of DEG C；Ultrasonic power 400w, frequency 25kHz；
(10) it is separated by filtration：Lixiviating solution is filtered, lixiviating solution and leaching residue is obtained；
(11) prepared by fermentation liquid：The pure water of 5 times of mass ratioes is added in the leaching residue in step (10), and is added residual
The glucose of slag mass ratio 5.0%, 2.0% yeast extract, 0.5% potassium dihydrogen phosphate, 0.02%MgSO4, 0.01%CaCl2, pH is adjusted to
7.0~7.2；
(12) aerobic fermentation：From liquid fermentation tank, liquid amount volume ratio is 60~70%, inoculation bacillus megaterium and
Aspergillus niger, inoculum concentration is respectively 4%, 2%, controls 28~37 DEG C of fermentation temperature, 150~300rpm of rotating speed；In this fermentation condition
Bottom fermentation 12h；
(13) anaerobic fermentation：After aerobic fermentation terminates, streptococcus thermophiluss and Lactobacillus bulgaricus are inoculated with, inoculum concentration is
4%, 28~37 DEG C of fermentation temperature, temperature control anaerobic fermentation 12h；
(14) merge：Fermentation liquid in lixiviating solution and step (13) in enzymolysis solution in step (8), step (10) is closed
And；
(15) cryoconcentration：The enhancing agent solution after step (14) merging is concentrated under cryogenic；
(16) adsorption dry：With the wheat bran absorption of 1~1.5 times of mass ratio after concentration, in 40~60 DEG C of blast driers
It is dried, moisture Control is 8~10%；
(17) crush：The dried mesh of product pulverizer 60 is sieved crushing, Penaeus vannamei is obtained final product and is added with feedstuff
Agent.
Embodiment 3：
Salicornia Bigelovii Torr. penaeus vannamei boone feed additive prepared by a kind of joint enzymatic isolation method and fermentation method, concrete preparation process is such as
Under：
(1) immersion is crushed：Deimpurity Salicornia Bigelovii Torr. cane will be gone to crush, cross 100 mesh sieves, be subsequently adding 2 times of mass ratioes pure
Water purification soaks 3h；
(2) steaming and decocting：By the Salicornia Bigelovii Torr. suspension steaming and decocting 1h after immersion, then decline in air-proof condition and warm to room temperature；
(3) separate：Cooking liquor is carried out into solid-liquid separation, Salicornia Bigelovii Torr. cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(4) become thoroughly decomposed naturally：Control Salicornia Bigelovii Torr. cane powder water content 40~50%, 30~50 DEG C of temperature control, become thoroughly decomposed 50d naturally；
(5) two-step fermentation enzymolysis：Naturally become thoroughly decomposed after end, add Salicornia Bigelovii Torr. mass ratio 2% cellulase and 1% it is non-
Starch-polysaccharides enzyme, and be inoculated with the salt tolerant Alkaliphilic bacillus of Salicornia Bigelovii Torr. mass ratio 2%, in 28~35 DEG C of condition bottom fermentation 5d, 12
Hour stirring is once；Salicornia Bigelovii Torr. yeast powder is obtained final product after fermentation ends；
(6) co-grinding：Each raw material is pressed into 30 parts of Salicornia Bigelovii Torr. Powder, 20 parts of Herba Taraxaci, 8 parts of Radix Et Caulis Acanthopanacis Senticosi, 12 parts of Herba Dendrobii, Fructus Hordei Germinatus
6 parts, 5 parts of spirulina, 6 parts of Radix Platycodoniss, 8 parts of Radix Glycyrrhizae, 3 parts of Radix Rehmanniae Preparata, 7 parts of Herba Imperatae, the mixing of 3 parts of ratios of Radix Gentianae, then crushed 100 mesh
Sieve；Add the pure water of 3 times of raw materials quality；
(7) digest：Cellulase, 2% non-starch polysaccharide enzyme of herbal raw material mass ratio 3% are added, 40~60
Temperature control enzymolysis 8h, stirs once every 0.5h under the conditions of DEG C；
(8) it is separated by filtration：Enzymolysis solution is filtered, enzymolysis solution and enzymolysis residue is obtained；
(9) Ultrasonic Wave-Assisted Extraction：3 times of pure water, under ultrasonic assistant effect, 100 are added in enzymolysis residue
Backflow extraction 30min, ultrasonic power 400w, frequency 25kHz under the conditions of DEG C；Ultrasonic power 400w, frequency 25kHz；
(10) it is separated by filtration：Lixiviating solution is filtered, lixiviating solution and leaching residue is obtained；
(11) prepared by fermentation liquid：The pure water of 5 times of mass ratioes is added in the leaching residue in step (10), and is added residual
The glucose of slag mass ratio 3.0%, 3.0% yeast extract, 0.3% potassium dihydrogen phosphate, 0.02%MgSO4, 0.01%CaCl2, pH is adjusted to
7.0~7.2；
(12) aerobic fermentation：From liquid fermentation tank, liquid amount volume ratio is 60~70%, inoculation bacillus megaterium and
Aspergillus niger, inoculum concentration is respectively 2%, 1%, controls 28~37 DEG C of fermentation temperature, 150~300rpm of rotating speed；In this fermentation condition
Bottom fermentation 24h；
(13) anaerobic fermentation：After aerobic fermentation terminates, streptococcus thermophiluss and Lactobacillus bulgaricus are inoculated with, inoculum concentration is
2%, 28~37 DEG C of fermentation temperature, temperature control anaerobic fermentation 24h；
(14) merge：Fermentation liquid in lixiviating solution and step (13) in enzymolysis solution in step (8), step (10) is closed
And；
(15) cryoconcentration：The enhancing agent solution after step (14) merging is concentrated under cryogenic；
(16) adsorption dry：With the wheat bran absorption of 1~1.5 times of mass ratio after concentration, in 40~60 DEG C of blast driers
It is dried, moisture Control is 8~10%；
(17) crush：The dried mesh of product pulverizer 60 is sieved crushing, Penaeus vannamei is obtained final product and is added with feedstuff
Agent.
Hereinafter the technique effect that the application is obtained is illustrated with checking test：
Table 1 tests grouped table
The result of the test of table 2
Above-mentioned result of the test shows that the application feed additive achieves significant technique effect, more existing commercially available medium-height grass
Medicine feed additive significant difference (P<0.01), and result of the test show feed change composition can significantly reduce product effect (P<
0.01)。
Above example is merely to illustrate technical scheme, rather than is limited；Although with reference to aforementioned reality
Example is applied to being described in detail by invention, but for the person of ordinary skill of the art, still can be to aforementioned reality
Apply the technical scheme described in example to modify, or equivalent is carried out to which part technical characteristic；And to these modifications
Or replace, do not make the spirit and scope of the essence disengaging claimed technical solution of the invention of appropriate technical solution.
Claims (4)
1. the Salicornia Bigelovii Torr. penaeus vannamei boone feed additive that prepared by a kind of joint enzymatic isolation method and fermentation method, it is characterised in that by following
The raw material of mass ratio is made：30~50 parts of Salicornia Bigelovii Torr. Powder, 15~20 parts of Herba Taraxaci, 8~12 parts of Radix Et Caulis Acanthopanacis Senticosi, 8~12 parts of Herba Dendrobii, wheat
6~8 parts of bud, 3~5 parts of spirulina, 6~8 parts of Radix Platycodoniss, 6~8 parts of Radix Glycyrrhizae, 3~5 parts of Radix Rehmanniae Preparata, 5~7 parts of Herba Imperatae, 3~5 parts of Radix Gentianae.
2. the Salicornia Bigelovii Torr. penaeus vannamei boone feed addition that prepared by a kind of joint enzymatic isolation method according to claim 1 and fermentation method
Agent, it is characterised in that the raw material composition is preferably：40 parts of Salicornia Bigelovii Torr. Powder, 18 parts of Herba Taraxaci, 10 parts of Radix Et Caulis Acanthopanacis Senticosi, 10 parts of Herba Dendrobii,
7 parts of Fructus Hordei Germinatus, 4 parts of spirulina, 7 parts of Radix Platycodoniss, 7 parts of Radix Glycyrrhizae, 4 parts of Radix Rehmanniae Preparata, 6 parts of Herba Imperatae, 4 parts of Radix Gentianae.
3. the Salicornia Bigelovii Torr. penaeus vannamei boone feed addition that prepared by a kind of joint enzymatic isolation method according to claim 1 and fermentation method
Agent, it is characterised in that the Salicornia Bigelovii Torr. Powder pretreating process is as follows：
（1）Crush immersion：Deimpurity Salicornia Bigelovii Torr. cane will be gone to crush, cross 100 mesh sieves, and be subsequently adding 2 times of quality and compare pure water
3~5h of immersion；
（2）Steaming and decocting：By the Salicornia Bigelovii Torr. suspension 1~2h of steaming and decocting after immersion, then decline in air-proof condition and warm to room temperature；
（3）Separate：Cooking liquor is carried out into solid-liquid separation, Salicornia Bigelovii Torr. cane powder is obtained, the water after separation enters step（1）It is circulated
Utilize；
（4）Naturally become thoroughly decomposed：Control Salicornia Bigelovii Torr. cane powder water content 40~50%, 30~50 DEG C of temperature control, become thoroughly decomposed 30~50d naturally；
（5）Two-step fermentation is digested：Naturally become thoroughly decomposed after end, add the cellulase and 1% non-starch of Salicornia Bigelovii Torr. mass ratio 2% many
Carbohydrase, and the salt tolerant Alkaliphilic bacillus of Salicornia Bigelovii Torr. mass ratio 2~3% are inoculated with, in 28~35 DEG C of condition 3~5d of bottom fermentation, 12 is little
When stirring once；Salicornia Bigelovii Torr. yeast powder is obtained final product after fermentation ends.
4. the Salicornia Bigelovii Torr. penaeus vannamei boone feed addition that prepared by a kind of joint enzymatic isolation method according to claim 1 and fermentation method
Agent, it is characterised in that the feed additive is made by the steps：
（1）Co-grinding：Each raw material is mixed in proportion, 100 mesh sieves were then crushed；Add the pure water of 3 times of raw materials quality；
（2）Enzymolysis：Cellulase, 2~3% non-starch polysaccharide enzyme of herbal raw material mass ratio 3~5% are added, 40~60
Temperature control digests 4~8h under the conditions of DEG C, stirs once every 0.5h；
（3）It is separated by filtration：Enzymolysis solution is filtered, enzymolysis solution and enzymolysis residue is obtained；
（4）Ultrasonic Wave-Assisted Extraction：3~5 times of pure water, under ultrasonic assistant effect, 100 DEG C are added in enzymolysis residue
Under the conditions of backflow extraction 20~30min, ultrasonic power 400w, frequency 25kHz；Ultrasonic power 400w, frequency 25kHz；
（5）It is separated by filtration：Lixiviating solution is filtered, lixiviating solution and leaching residue is obtained；
（6）It is prepared by fermentation liquid：To step（5）In leaching residue in add the pure water of 5 times of mass ratioes, and add mass of residue
Than 3.0~5.0% glucoses, 2.0~3.0% yeast extracts, 0.3~0.5% potassium dihydrogen phosphate, 0.02%MgSO4、0.01%CaCl2, pH
It is adjusted to 7.0~7.2；
（7）Aerobic fermentation：From liquid fermentation tank, liquid amount volume ratio is 60~70%, is inoculated with bacillus megaterium and black fermented preparation
Mould, inoculum concentration is respectively 2~4%, 1~2%, controls 28~37 DEG C of fermentation temperature, 150~300rpm of rotating speed；In this fermentation condition
12~24h of bottom fermentation；
（8）Anaerobic fermentation：After aerobic fermentation terminates, inoculation streptococcus thermophiluss and Lactobacillus bulgaricus, inoculum concentration is 2~
4%, 28~37 DEG C of fermentation temperature, temperature control 12~24h of anaerobic fermentation；
（9）Merge：By step（3）In enzymolysis solution, step（5）In lixiviating solution and step（8）In fermentation liquid merge；
（10）Cryoconcentration：Under cryogenic by step（9）Enhancing agent solution after merging is concentrated；
（11）Adsorption dry：With the wheat bran absorption of 1~1.5 times of mass ratio after concentration, it is dried in 40~60 DEG C of blast driers,
Moisture Control is 8~10%；
（12）Crush：The dried mesh of product pulverizer 60 is sieved crushing, Penaeus vannamei feed additive is obtained final product.
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