CN106665333A - Artificial cross pollination method of radix pseudostellariae - Google Patents
Artificial cross pollination method of radix pseudostellariae Download PDFInfo
- Publication number
- CN106665333A CN106665333A CN201710055724.XA CN201710055724A CN106665333A CN 106665333 A CN106665333 A CN 106665333A CN 201710055724 A CN201710055724 A CN 201710055724A CN 106665333 A CN106665333 A CN 106665333A
- Authority
- CN
- China
- Prior art keywords
- flower
- phase
- radix pseudostellariae
- flowers
- artificial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/027—Apparatus for pollination
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses an artificial cross pollination method of radix pseudostellariae. The artificial cross pollination method comprises the following steps: (1) respectively carrying out isolated cultivation on male parents and female parents of the radix pseudostellariae in cross pollination; (2) erecting and covering thin films in male parent and female parent cultivation places of the radix pseudostellariae at 15 days before artificial cross pollination and 30 days after the artificial cross pollination; (3) removing III-stage and IV-stage flowers, which are not subjected to the artificial pollination, of all petalous flowers of all the female parents, and reserving I-stage and II-stage flowers of petalous flowers of healthy female parents; (4) selecting and picking II-stage flowers of petalous flowers of healthy male parents, collecting pollen and saving for standby use; (5) selecting the II-stage flowers of the petalous flowers of the healthy female parents, dipping a small amount of pollen by using a writing brush or a cotton swab, shaking off the pollen to stigmas, and pollinating the flowers one by one; (6) castrating the pollinated II-stage flowers of the petalous flowers of the female parents; (7) repeating the steps from the step (3) to the step (6) after the I-stage flowers of the petalous flowers of the female parents enter a stage II. According to the artificial cross pollination method disclosed by the invention, the artificial cross pollination efficiency of the radix pseudostellariae is effectively improved, the cross fruiting rate is increased, and cross breeding work of the radix pseudostellariae is promoted to be carried out.
Description
Technical field
The present invention relates to agricultural technology field, and in particular to a kind of radix pseudostellariae artificial hybridization pollinating method.
Background technology
Radix pseudostellariae is perennial medicinal plant, is traditional strengthening by means of tonics Chinese medicine, is used as medicine with root tuber, strong with QI invigorating
Spleen, it is promoting production of body fluid and nourishing the lung the effect of.Radix pseudostellariae is mainly root tuber breeding and seminal propagation, and the multiplex root tuber of artificial cultivation carries out asexual numerous
Grow, the accumulation of radix pseudostellariae disease, germplasm is degenerated.Radix pseudostellariae seminal propagation can cultivate virus-free seedling, can effectively prevent germplasm from moving back
Change.
Hybridization is that the merit of two or more kinds is concentrated in together by mating, then by selection and is cultivated,
The method for obtaining new varieties, is breeding important method, and the new varieties of seed selection generally embody hybrid vigour.The important work of plant hybridization
Work is to carry out artificial pollination, is plant pollen to be sent on column cap to improve the technical measures of setting percentage by artificial means.
The colored both sexes of radix pseudostellariae, two types.Having hemp nettle at the top of radix pseudostellariae can bloom, 10 pieces of flower pesticide, 3, column cap, can manage it from flower
Pollination and cross-pollination, the apetalous flower of base portion do not bloom, and carry out strict self-pollination.Radix pseudostellariae plant is shorter, and flower is small, flower
Handle is thin, using conventional plant hybridization pollinating method, exists and is difficult to carry out bagging to the flower of emasculation, and easily flower is rolled over after bagging
The problems such as damage.
Ripe when radix pseudostellariae has the stamen of hemp nettle different with gynoecium, gynoecium is first ripe, when the petal of flower does not open, gynoecium
Column cap stretched out outside petal, and expanded；When petal just opens, the column cap of gynoecium has been expanded in sparkling and crystal-clear bright shape, is subjected to
Pollen, but now, the flower pesticide of stamen does not ftracture also, and illustrating the hemp nettle that has the tendency of of radix pseudostellariae has cross-pollination.Radix pseudostellariae has
Hemp nettle is bloomed, and during fine day, flower pesticide just ftractures for 9 points or so in the morning；When cloudy, flower pesticide just has part to ftracture at noon, and
The crack of anther sac is smaller；During the rainy day, flower pesticide does not ftracture typically.Radix pseudostellariae flowering time is short, and petal is opened into withers generally only
24 hours, pistil stigma can pollination time it is also shorter.
In plant hybridization pollination, because its Parent flowering time is different, paternal pollen need to often be preserved.During preservation,
Low temperature drying treatment can reduce pollen respiration and other physiological functions, be conducive to the pollen long period to maintain vigour, and improve
Artificial hybridization pollination efficiency.
The setting percentage of radix pseudostellariae base portion apetalous flower is high, and fruit generally has 5-10 seed；And there is hemp nettle at top by weather
Influence change is very big, if season of flowers is generally fine day, setting percentage is higher, if season of flowers is generally rainy weather, the flower pesticide of stamen
It is difficult to ftracture, then setting percentage is relatively low, in addition it is shaky.
After radix pseudostellariae seed carries out appropriate low-temperature treatment, germination rate is higher, has certain in production using seminal propagation
Application.But due to there is problems with：（1）Radix pseudostellariae flower is smaller, and flower pesticide is more, and column cap is small, is difficult to carry out emasculation, pollination et al.
Work is operated；（2）Radix pseudostellariae anthocaulus is thin, and artificial bagging easily loses flower；（3）Radix pseudostellariae flowering time is short, and stamen and gynoecium are not
Simultaneously ripe, the pollen collecting of stamen is more difficult, pistil stigma can pollination time it is shorter；（4）Radix pseudostellariae is bloomed by weather shadow
Ring very big, rainy weather setting percentage is low, or even shaky；Therefore radix pseudostellariae artificial hybridization pollination work is difficult to carry out.
The content of the invention
It is an object of the invention to be directed to state of the art, there is provided a kind of radix pseudostellariae artificial hybridization pollinating method, solve
Radix pseudostellariae artificial pollination bagging easily loses flower, pollen collecting and difficult treatment, pistil stigma it is small, can pollination time be short, go
Male speed is slow, the low key issue of rainy weather setting percentage.
To achieve the above object, the present invention is adopted the following technical scheme that：
The developmental stage that radix pseudostellariae has hemp nettle was divided into for I-IV phase, I phase flower refers to that petal does not open, flower pesticide does not expose；II phase
Flower refers to that petal has opened, and stamen development is ripe, and flower pesticide exposes and do not ftracture, and column cap stretches out and expands；III phase flower refers to
Flower pesticide has been ftractureed, and petal is not withered；IV phase flower refers to that petal has been withered.
Radix pseudostellariae artificial hybridization pollinating method is comprised the following steps：
1) isolation cultivation：Male parent to radix pseudostellariae hybridization pollination carries out isolating cultivation respectively with female parent, prevents biology hybrid；
2) built epiphragma：Artificial hybridization is pollinated first 15 days to 30 days after pollination, in radix pseudostellariae male parent, maternal cultivation plot built,
Cover film on frame；Fine day is met, film is pulled open；It is pre- overcast and rainy, film covering.
3）Selection flower：Extracing all female parents has III phase of the non-artificial pollination of hemp nettle, IV phase flower, collects and takes away, and protects
Staying healthy female parent has the phase of hemp nettle I and II phase flower, and the unhealthy female parent of excision is all of hemp nettle；
4）Prepare pollen：Selecting and win healthy male parent has II phase of hemp nettle flower, takes back interior, extracts calyx and petal, remaining
It is partially placed into clean uncovered culture dish, is placed in freeze-drying lyophilization in 1-4 DEG C of environment, after being taken out after most of anther dehiscence, shakes off
Pollen, collects pollen in clean brown bottle, is put into 1-4 DEG C of environment and saves backup；
5）Artificial hybridization is pollinated：Artificial hybridization is pollinated first 1 hour and takes out step 4）The pollen of preservation, is gradually restored to room temperature, choosing
Selecting healthy female parent has II phase of hemp nettle flower, and a little pollen is accounted for writing brush or cotton swab, and pollen is shaken off into the healthy female parent valve
Spend on the column cap of II phase flower, carry out flower pollination one by one rapidly；
6）Artificial emasculation：After artificial hybridization pollination terminates, there is II phase of hemp nettle flower to carry out emasculation to the female parent pollinated at once, it is small
The heart is clean by the flower pesticide removal of stamen, it is impossible to touches and hinders pistil stigma, flower pesticide is collected and taken away；
7）After female parent has I phase of hemp nettle flower to enter for II phase, 3 are repeated the above steps）- step 6）.
The step 2）The time of built epiphragma is 15 days to 30 days after pollination before artificial hybridization pollination.
The step 4）The pollen saved backup in 1-4 DEG C of environment, used in 5 days, flower of the holding time more than 5 days
Powder is not used.
The step 5）Writing brush or cotton swab are not contacted with pistil stigma, in order to avoid column cap is impaired, influence Seed Development.
Further, step 6）Afterwards, the female parent that part has been pollinated has II phase of hemp nettle flower, has little time emasculation and has been enter into III
Phase, then there is III phase of hemp nettle flower to extract the female parent, collect and take away.
The present invention, the healthy male parent, female parent refer to normal plant strain growth, disease and degradation phenomena, flower do not occur
Development is normal, and each partial structure spent is complete, form is normal；The unhealthy female parent refers to that plant disease, short and small degeneration occurs
Etc. phenomenon.
The present invention uses above technical scheme, there is provided a kind of radix pseudostellariae artificial hybridization pollinating method, is cultivated by isolating
The male parent and female parent of radix pseudostellariae hybridization pollination, prevent pollen from ganging up, after emasculation and artificial pollination, flower not bagging, it is to avoid set
Bag easily makes the problem that flower is lost；Rain cover is covered using film shed, flower normal development is effectively facilitated, flower after pollination is improved
Setting percentage；It is a large amount of from male parent garden to win whole flower, calyx and petal are removed, low temperature drying is carried out, promote anther dehiscence, can obtain
To pollinia, pollen collecting efficiency is drastically increased；Pollen is occupied using writing brush or cotton swab, is shaken off on column cap, it is to avoid
Column cap is damaged, and also solves the problem that column cap is small, be difficult pollination；Using the method for first pollination, rear emasculation, gynoecium post is effectively solved
Head can pollination time is short, artificial emasculation is slow problem, substantially reduce the self-pollination probability that anther dehiscence causes.
The present invention has the feature of hemp nettle development by conscientiously observation radix pseudostellariae, is fully first stretched out beneficial to its column cap, swollen
Greatly, can pollinate, the uncracked time feature of flower pesticide, take Parent isolate cultivation, film shed rain cover, largely collection pollen,
First pollination, the operating process of rear emasculation, form the specific process of radix pseudostellariae artificial hybridization pollination, effectively increase radix pseudostellariae artificial
Hybridization pollination efficiency, improves Cross fertile rate.
Specific embodiment
The present invention is described in further detail below in conjunction with specific embodiment.
A kind of radix pseudostellariae artificial hybridization pollinating method, I-IV phase, I phase flower are divided into by the developmental stage that radix pseudostellariae has hemp nettle
Refer to that petal does not open, flower pesticide does not expose；II phase flower refers to that petal has opened, and stamen development is ripe, and flower pesticide exposes and do not open
Split, column cap stretches out and expands, and color of the stigma turns white, it is sparkling and crystal-clear bright；III phase flower refers to that flower pesticide has ftractureed, and petal is not withered；Ⅳ
Phase flower refers to that petal has been withered；Methods described specifically includes following steps：
1) isolation cultivation：Male parent to radix pseudostellariae hybridization pollination carries out isolating cultivation respectively with female parent, it is to be ensured that be effectively isolated,
Prevent biology hybrid；
2) built epiphragma：Artificial hybridization is pollinated first 15 days to 30 days after pollination, in radix pseudostellariae male parent, maternal cultivation plot built,
Cover film on frame；Fine day is met, film is pulled open；It is pre- overcast and rainy, film covering.
3）Selection flower：Extracing all female parents has III phase of the non-artificial pollination of hemp nettle, IV phase flower, collects and takes away, and protects
Staying healthy female parent has the phase of hemp nettle I and II phase flower, and the unhealthy female parent of excision is all of hemp nettle；
4）Prepare pollen：Selecting and win healthy male parent has II phase of hemp nettle flower, takes back interior, extracts calyx and petal, remaining
It is partially placed into clean uncovered culture dish, is placed in freeze-drying lyophilization in 1-4 DEG C of environment, after being taken out after most of anther dehiscence, shakes off
Pollen, collects pollen in clean brown bottle, is put into 1-4 DEG C of environment and saves backup, and is used in 5 days, and the holding time surpasses
The pollen for spending 5 days is not used；
5）Artificial hybridization is pollinated：Artificial hybridization is pollinated first 1 hour and takes out step 4）The pollen of preservation, is gradually restored to room temperature, choosing
Selecting healthy female parent has II phase of hemp nettle flower, and a little pollen is accounted for writing brush or cotton swab, and pollen is shaken off into the healthy female parent valve
Spend on the column cap of II phase flower, writing brush or cotton swab are not contacted with column cap, carry out flower pollination one by one rapidly；
6）Artificial emasculation：After artificial hybridization pollination terminates, there is II phase of hemp nettle flower to carry out emasculation to the female parent pollinated at once, it is small
The heart is clean by the flower pesticide removal of stamen, it is impossible to touches and hinders pistil stigma, flower pesticide is collected and taken away；
7）The female parent that part has been pollinated has II phase of hemp nettle flower, has little time emasculation and had been enter into for III phase, then the female parent is had into hemp nettle III
Phase flower is extractd, and is collected and is taken away.
8）After female parent has I phase of hemp nettle flower to enter for II phase, 3 are repeated the above steps）- step 7）.
The flower of radix pseudostellariae is smaller, and flower pesticide is more, and artificial emasculation speed is slow, but artificial pollination speed is fast, radix pseudostellariae gynoecium post
Head can pollination time it is shorter, the present invention using first pollination, the method for rear emasculation, allow the pollen very first time of hybridization to occupy and can award
The pistil stigma of powder, promotes the sprouting of hybridization pollen, improves hybridization efficiency.The first emasculation of the method and routine, afterwards pollination
Method is compared, and artificial hybridization pollination efficiency can improve more than 2 times.
Radix pseudostellariae top has hemp nettle to open, and during the rainy day, the flower pesticide of its stamen does not ftracture typically, if continuous rainy weather, flower
Piece mostly abortion, setting percentage be less than 10%, even zero.The present invention is opened by built epiphragma rain cover, radix pseudostellariae flower,
Natural seed setting rate about 50% or so；Pollen is greatly improved by artificial preparation, the setting percentage of artificial hybridization pollination up to 90% or so
Artificial hybridization setting percentage.
Claims (5)
1. a kind of radix pseudostellariae artificial hybridization pollinating method, it is characterised in that：The developmental stage that radix pseudostellariae has hemp nettle is divided into I-IV
Phase, I phase flower refers to that petal does not open, flower pesticide does not expose；II phase flower refers to that petal has opened, and stamen development is ripe, flower pesticide
Expose and do not ftracture, column cap stretches out and expands；III phase flower refers to that flower pesticide has ftractureed, and petal is not withered；IV phase flower refers to flower
Valve has been withered；The artificial hybridization pollinating method is comprised the following steps：
1) isolation cultivation：Male parent to radix pseudostellariae hybridization pollination carries out isolating cultivation respectively with female parent, prevents biology hybrid；
2) built epiphragma：In radix pseudostellariae male parent, maternal cultivation plot built, cover film on frame；Only when fine day, by film
Pull open；
3）Selection flower：Extracing all female parents has III phase of the non-artificial pollination of hemp nettle, IV phase flower, collects and takes away, and retains strong
Health female parent has the phase of hemp nettle I and II phase flower, and the unhealthy female parent of excision is all of hemp nettle；
4）Prepare pollen：Selecting and win healthy male parent has II phase of hemp nettle flower, takes back interior, extracts calyx and petal, remaining
It is partially placed into clean uncovered culture dish, is placed in freeze-drying lyophilization in 1-4 DEG C of environment, after being taken out after most of anther dehiscence, shakes off
Pollen, collects pollen in clean brown bottle, is put into 1-4 DEG C of environment and saves backup；
5）Artificial hybridization is pollinated：Artificial hybridization is pollinated first 1 hour and takes out step 4）The pollen of preservation, is gradually restored to room temperature, choosing
Selecting healthy female parent has II phase of hemp nettle flower, and a little pollen is accounted for writing brush or cotton swab, and pollen is shaken off into the healthy female parent valve
Spend on the column cap of II phase flower, flower pollination is carried out one by one；
6）Artificial emasculation：After artificial hybridization pollination terminates, there is II phase of hemp nettle flower to carry out emasculation to the female parent pollinated at once, will
Flower pesticide is collected and taken away；
7）After female parent has I phase of hemp nettle flower to enter for II phase, 3 are repeated the above steps）- step 6）.
2. a kind of radix pseudostellariae artificial hybridization pollinating method according to claim 1, it is characterised in that：The step 2）Built
The time of epiphragma is 15 days to 30 days after pollination before artificial hybridization pollination.
3. a kind of radix pseudostellariae artificial hybridization pollinating method according to claim 1, it is characterised in that：The step 4）In 1-
The pollen saved backup in 4 DEG C of environment, used in 5 days.
4. a kind of radix pseudostellariae artificial hybridization pollinating method according to claim 1, it is characterised in that：The step 5）Writing brush
Or cotton swab is not contacted with column cap.
5. a kind of radix pseudostellariae artificial hybridization pollinating method according to claim 1, it is characterised in that：The step 6）It
Afterwards, the female parent that part has been pollinated has II phase of hemp nettle flower, if also non-emasculation had been enter into for III phase and has III phase of hemp nettle flower as female parent,
Then there is III phase of hemp nettle flower to extract the female parent, collect and take away.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710055724.XA CN106665333B (en) | 2017-01-25 | 2017-01-25 | A kind of radix pseudostellariae artificial hybridization pollinating method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710055724.XA CN106665333B (en) | 2017-01-25 | 2017-01-25 | A kind of radix pseudostellariae artificial hybridization pollinating method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106665333A true CN106665333A (en) | 2017-05-17 |
| CN106665333B CN106665333B (en) | 2018-11-13 |
Family
ID=58860048
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710055724.XA Active CN106665333B (en) | 2017-01-25 | 2017-01-25 | A kind of radix pseudostellariae artificial hybridization pollinating method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106665333B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960010A (en) * | 2014-04-25 | 2014-08-06 | 贵州三泓药业股份有限公司 | Construction method of pseudostellaria heterophylla germplasm resource nurseries |
-
2017
- 2017-01-25 CN CN201710055724.XA patent/CN106665333B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960010A (en) * | 2014-04-25 | 2014-08-06 | 贵州三泓药业股份有限公司 | Construction method of pseudostellaria heterophylla germplasm resource nurseries |
Non-Patent Citations (3)
| Title |
|---|
| KYUNG CHOI ET AL.: "Natural hybridization between Pseudostellaria davidii and Pseudostellaria palibiniana (Caryophyllaceae）", 《PLANT SPECIES BIOLOGY》 * |
| 韩怡等: "太子参花粉活力和柱头可授性研究", 《种子》 * |
| 韩怡等: "江苏省地产药材太子参良种选育研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106665333B (en) | 2018-11-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102960239A (en) | Cultivating method of red-flesh apples | |
| Okubo | Growth cycle and dormancy in plants. | |
| CN105409758A (en) | Artificial emasculation and hybrid control pollination method of robinia pseudoacacia L. | |
| CN107432243B (en) | A kind of selection of cold-resistant, resistance to bolting cabbage | |
| CN103975848B (en) | Breeding and cultivating method for early-matured and disease-resistant brassica oleracea | |
| CN113331057B (en) | Method for inducing somatic embryogenesis by using rubber tree calyx | |
| CN105409756A (en) | Artificial hybridization method for pond-cultured peanuts in elevation stand | |
| CN104855274A (en) | Artificial pollination method for litchi | |
| CN109566404A (en) | A kind of sponge gourd cross-breeding method in two seasons of one year | |
| CN105123496A (en) | Paddy rice hybridization method | |
| CN103931485B (en) | A kind of cross breeding method of okra | |
| CN115362932A (en) | Pineapple film-covering moisture-proof hybridization method | |
| Vander Kloet et al. | The taxonomy of Vaccinium section Hemimyrtillus | |
| CN106818226B (en) | Multi-variety top-grafting head-changing hybrid seed production method for persimmon trees | |
| CN104957028A (en) | High-yield hybrid seed production method of cotton | |
| CN106665333B (en) | A kind of radix pseudostellariae artificial hybridization pollinating method | |
| CN107094615A (en) | A kind of hybridizing method for overcoming lily interspecific hybridization obstacle | |
| CN108739364B (en) | Method for improving seed production efficiency of self-incompatible gramineous plant hybridization | |
| CN111011206A (en) | Method for cultivating new pepper variety by distant hybridization grafting | |
| CN104982329A (en) | Artificial emasculation hybrid seed production method for okra | |
| CN112293258A (en) | Method for rapidly obtaining pepper homozygous diploid | |
| CN113068606A (en) | Artificial emasculation and breeding method for indoor planting of chicory | |
| CN106879457B (en) | A kind of selection of high yielding soybeans kind | |
| CN1579145A (en) | Method of artificial hybridizing ang pollinating lotus exempting from male | |
| CN111758562B (en) | Artificial pollination method for ivory plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |