CN103960010A - Construction method of pseudostellaria heterophylla germplasm resource nurseries - Google Patents
Construction method of pseudostellaria heterophylla germplasm resource nurseries Download PDFInfo
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- CN103960010A CN103960010A CN201410170329.2A CN201410170329A CN103960010A CN 103960010 A CN103960010 A CN 103960010A CN 201410170329 A CN201410170329 A CN 201410170329A CN 103960010 A CN103960010 A CN 103960010A
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Abstract
The invention discloses a construction method of pseudostellaria heterophylla germplasm resource nurseries. The construction method comprises construction of the germplasm resource nurseries, germplasm selection and germplasm protection. According to construction of the germplasm resource nurseries, an isolation strip with the length being more than 500 m should be arranged between a lot where the pseudostellaria heterophylla germplasm resource nurseries are constructed and a production lot according to the technical requirements for pseudostellaria heterophylla propagation; the area of each germplasm resource storage nursery is 10 m<2>, and each planting box surface is 10 m long and 1 m wide, and the natural isolation distance between every two germplasm resource storage nurseries is 1.5 m. By constructing the pseudostellaria heterophylla germplasm resource nurseries, pseudostellaria heterophylla in the germplasm resource nurseries are not allowed to propagate, and germplasm of the pseudostellaria heterophylla in each germplasm resource storage nursery cannot change; on the condition of same-place planting, biological character observation is conducted, all parts of germplasm in the germplasm resource nurseries are purified and optimized, convenience is brought to sampling studying for researchers, therefore, a new variety can be cultured for conducting fine breed propagation and the requirements for field production are met.
Description
Technical field
The present invention relates to a kind of building method of Germplasm Resources, specifically the building method of radix pseudostellariae Germplasm Resources.
Background technology
Although the cultivated area of national radix pseudostellariae expands year by year, but because radix pseudostellariae germplasm seriously this problem of degenerating can not get basic solution, do not utilize the means of science to cultivate, select the disease-resistant variety of good quality and high output, radix pseudostellariae volume increase, the target increasing income cannot be accomplished.Traditional Chinese medicine germplasm is the important means of production in traditional Chinese medicine production process, and traditional Chinese medicine standardization is produced with breed breeding most important, must carry out the preservation of collection, living specimen and the seed of germ plasm resource; Sympatric species is planted and carries out biological character observation, Germplasm Identification and Genetic relationship; Study differentiation situation and quality discrepancy between each germplasm; Each germplasm biology, output, disease resistance, resistance, seed testing quarantine and pharmacy quality evaluation; Germplasm purifying and preferably, rearing new variety and breed improvement, gene analyze and germplasm Techniques of preserving research etc.
Summary of the invention
The object of the present invention is to provide the building method of radix pseudostellariae Germplasm Resources, to solve the problem proposing in above-mentioned background technology.
For achieving the above object, the invention provides following technical scheme:
The building method of radix pseudostellariae Germplasm Resources, comprises the construction of Germplasm Resources, selection and the Conservation method of germplasm;
The construction of Germplasm Resources: by the requirement of radix pseudostellariae germplasm raising technology, between the plot of radix pseudostellariae Germplasm Resources construction and production plot, isolation strip more than 500m should be set; The area that every a germ plasm resource is preserved garden is 10 ㎡, plantation railway carriage or compartment face length 10m, and wide 1m, and the natural isolation distance that every a germ plasm resource is preserved between garden is 1.5 m;
The selection of germplasm: select loose, healthy and strong, bud head is complete, not damaged, disease-free kind root or seed, and every a germ plasm resource is preserved the radix pseudostellariae of only planting single variety in garden; Before plantation, need disinfection, wherein 0.5% formalin solution seed soaking 20-30 minute for shot hole, tar spot and balck shank, soaks rear waterlogging and goes out vexed 6-8 hour, can play bactericidal action;
Conservation method: since seedling stage to whole breeding time, not only will prevent artificial mechanical admixture, also will strictly carry out germplasm isolation and controlled pollination work; Within 5-7 days, regularly remove the normal bud of each germplasm; prevent the formation of normal capsule; the each part of radix pseudostellariae germ plasm resource that germ plasm resource is preserved in garden is carried out impurity elimination roguing, carries out biological character observation under the condition of planting sympatric species, and the germplasm in each Germplasm Resources is carried out to purifying and preferred.
As the further scheme of the present invention: in the construction of described Germplasm Resources, except germplasm preserve railway carriage or compartment face vegetation because of plantation need destroyed, other vegetation all should be kept intact.
As the further scheme of the present invention: in the selection of described germplasm, 0.5% copper-bath seed soaking potato sterilization in 2 hours for balck shank.
As further scheme of the present invention: in the selection of described germplasm, radix pseudostellariae ploughs under soil layer by table soil after planting and being made into 800 times of liquid spray furrow faces with 0.5 kilogram of 50% phoxim emulsifiable concentrate in first 15 days.
Compared with prior art, the invention has the beneficial effects as follows: by setting up radix pseudostellariae Germplasm Resources, to loose, healthy and strong, bud head is complete, not damaged, disease-free kind root or seed are planted, and do not allow the radix pseudostellariae in Germplasm Resources breed, the germplasm that every a germ plasm resource is preserved the radix pseudostellariae in garden can not change; The each part of radix pseudostellariae germ plasm resource of simultaneously germ plasm resource being preserved in garden is carried out impurity elimination roguing, under the condition of planting sympatric species, carry out biological character observation, by each part of germplasm purifying and preferred in Germplasm Resources, being convenient to scientific research personnel takes a sample to research, and then cultivate new varieties carry out stock breeding expand numerous, meet Production of Large Fields demand.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiment.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, the building method of radix pseudostellariae Germplasm Resources, comprises the construction of Germplasm Resources, selection and the Conservation method of germplasm;
Concrete building method: by the requirement of radix pseudostellariae germplasm raising technology, between the plot of radix pseudostellariae Germplasm Resources construction and production plot, isolation strip more than 500m should be set; The area that every a germ plasm resource is preserved garden is 10 ㎡, plantation railway carriage or compartment face length 10m, wide 1m; The natural isolation distance that every a germ plasm resource is preserved between garden is 1.5 m, except germplasm preserve railway carriage or compartment face vegetation because of plantation need destroyed, other vegetation all should be kept intact, and gives full play to natural, the suitable concealment condition of woodland and the advantage of higher relative air humidity reaches the object that radix pseudostellariae germ plasm resource is preserved;
The selection of germplasm: select loose, healthy and strong, bud head is complete, not damaged, disease-free kind root or seed, and every a germ plasm resource is preserved the radix pseudostellariae of only planting single variety in garden; Radix pseudostellariae ploughs under soil layer by table soil after planting and being made into 800 times of liquid spray furrow faces with 0.5 kilogram of 50% phoxim emulsifiable concentrate in first 15 days; In order to prevent that kind of root or Seed-Borne from dying, and needs disinfection before plantation; Shot hole, tar spot and balck shank can, with 0.5% formalin solution seed soaking 20 minutes, be soaked rear waterlogging and go out vexed 6 hours, can play bactericidal action; Balck shank also can be with the sterilization in 2 hours of 0.5% copper-bath seed soaking potato;
Conservation method: since seedling stage to whole breeding time, except preventing artificial mechanical admixture, for the hybridization degeneration that prevents that insect cross pollination from causing, strictly carry out germplasm isolation and controlled pollination work, prevent natural crossing; Within 5 days, regularly remove the normal bud of each germplasm; prevent the formation of normal capsule; the each part of radix pseudostellariae germ plasm resource that germ plasm resource is preserved in garden is carried out impurity elimination roguing; under the condition of planting sympatric species, carry out biological character observation; by each part of germplasm purifying and preferred in Germplasm Resources; cultivate new varieties carry out stock breeding expand numerous, meet Production of Large Fields demand.
Embodiment 2
The building method of radix pseudostellariae Germplasm Resources, comprises the construction of Germplasm Resources, selection and the Conservation method of germplasm;
Concrete building method: by the requirement of radix pseudostellariae germplasm raising technology, between the plot of radix pseudostellariae Germplasm Resources construction and production plot, isolation strip more than 500m should be set; The area that every a germ plasm resource is preserved garden is 10 ㎡, plantation railway carriage or compartment face length 10m, wide 1m; The natural isolation distance that every a germ plasm resource is preserved between garden is 1.5 m, except germplasm preserve railway carriage or compartment face vegetation because of plantation need destroyed, other vegetation all should be kept intact, and gives full play to natural, the suitable concealment condition of woodland and the advantage of higher relative air humidity reaches the object that radix pseudostellariae germ plasm resource is preserved;
The selection of germplasm: select loose, healthy and strong, bud head is complete, not damaged, disease-free kind root or seed, and every a germ plasm resource is preserved the radix pseudostellariae of only planting single variety in garden; Radix pseudostellariae ploughs under soil layer by table soil after planting and being made into 800 times of liquid spray furrow faces with 0.5 kilogram of 50% phoxim emulsifiable concentrate in first 15 days; In order to prevent that kind of root or Seed-Borne from dying, and needs disinfection before plantation; Shot hole, tar spot and balck shank can, with 0.5% formalin solution seed soaking 25 minutes, be soaked rear waterlogging and go out vexed 7 hours, can play bactericidal action; Balck shank also can be with the sterilization in 2 hours of 0.5% copper-bath seed soaking potato;
Conservation method: since seedling stage to whole breeding time, except preventing artificial mechanical admixture, for the hybridization degeneration that prevents that insect cross pollination from causing, strictly carry out germplasm isolation and controlled pollination work, prevent natural crossing; Within 6 days, regularly remove the normal bud of each germplasm; prevent the formation of normal capsule; the each part of radix pseudostellariae germ plasm resource that germ plasm resource is preserved in garden is carried out impurity elimination roguing; under the condition of planting sympatric species, carry out biological character observation; by each part of germplasm purifying and preferred in Germplasm Resources; cultivate new varieties carry out stock breeding expand numerous, meet Production of Large Fields demand.
Embodiment 3
The building method of radix pseudostellariae Germplasm Resources, comprises the construction of Germplasm Resources, selection and the Conservation method of germplasm;
Concrete building method: by the requirement of radix pseudostellariae germplasm raising technology, between the plot of radix pseudostellariae Germplasm Resources construction and production plot, isolation strip more than 500m should be set; The area that every a germ plasm resource is preserved garden is 10 ㎡, plantation railway carriage or compartment face length 10m, wide 1m; The natural isolation distance that every a germ plasm resource is preserved between garden is 1.5 m, except germplasm preserve railway carriage or compartment face vegetation because of plantation need destroyed, other vegetation all should be kept intact, and gives full play to natural, the suitable concealment condition of woodland and the advantage of higher relative air humidity reaches the object that radix pseudostellariae germ plasm resource is preserved;
The selection of germplasm: select loose, healthy and strong, bud head is complete, not damaged, disease-free kind root or seed, and every a germ plasm resource is preserved the radix pseudostellariae of only planting single variety in garden; Radix pseudostellariae ploughs under soil layer by table soil after planting and being made into 800 times of liquid spray furrow faces with 0.5 kilogram of 50% phoxim emulsifiable concentrate in first 15 days; In order to prevent that kind of root or Seed-Borne from dying, and needs disinfection before plantation; Shot hole, tar spot and balck shank can, with 0.5% formalin solution seed soaking 30 minutes, be soaked rear waterlogging and go out vexed 8 hours, can play bactericidal action; Balck shank also can be with the sterilization in 2 hours of 0.5% copper-bath seed soaking potato;
Conservation method: since seedling stage to whole breeding time, except preventing artificial mechanical admixture, for the hybridization degeneration that prevents that insect cross pollination from causing, strictly carry out germplasm isolation and controlled pollination work, prevent natural crossing; Within 7 days, regularly remove the normal bud of each germplasm; prevent the formation of normal capsule; the each part of radix pseudostellariae germ plasm resource that germ plasm resource is preserved in garden is carried out impurity elimination roguing; under the condition of planting sympatric species, carry out biological character observation; by each part of germplasm purifying and preferred in Germplasm Resources; cultivate new varieties carry out stock breeding expand numerous, meet Production of Large Fields demand.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned example embodiment, and in the situation that not deviating from spirit of the present invention or essential characteristic, can realize the present invention with other concrete form.Therefore, no matter from which point, all should regard embodiment as exemplary, and be nonrestrictive, scope of the present invention is limited by claims instead of above-mentioned explanation, is therefore intended to all changes that drop in the implication and the scope that are equal to important document of claim to include in the present invention.
In addition, be to be understood that, although this specification is described according to embodiment, but be not that each embodiment only comprises an independently technical scheme, this narrating mode of specification is only for clarity sake, those skilled in the art should make specification as a whole, and the technical scheme in each embodiment also can, through appropriately combined, form other embodiments that it will be appreciated by those skilled in the art that.
Claims (4)
1. the building method of radix pseudostellariae Germplasm Resources, comprises the construction of Germplasm Resources, selection and the Conservation method of germplasm, it is characterized in that:
The construction of Germplasm Resources: by the requirement of radix pseudostellariae germplasm raising technology, between the plot of radix pseudostellariae Germplasm Resources construction and production plot, isolation strip more than 500m should be set; The area that every a germ plasm resource is preserved garden is 10 ㎡, plantation railway carriage or compartment face length 10m, and wide 1m, and the natural isolation distance that every a germ plasm resource is preserved between garden is 1.5 m;
The selection of germplasm: select loose, healthy and strong, bud head is complete, not damaged, disease-free kind root or seed, and every a germ plasm resource is preserved the radix pseudostellariae of only planting single variety in garden; Before plantation, need disinfection, wherein 0.5% formalin solution seed soaking 20-30 minute for shot hole, tar spot and balck shank, soaks rear waterlogging and goes out vexed 6-8 hour, can play bactericidal action;
Conservation method: since seedling stage to whole breeding time, not only will prevent artificial mechanical admixture, also will strictly carry out germplasm isolation and controlled pollination work; Within 5-7 days, regularly remove the normal bud of each germplasm; prevent the formation of normal capsule; the each part of radix pseudostellariae germ plasm resource that germ plasm resource is preserved in garden is carried out impurity elimination roguing, carries out biological character observation under the condition of planting sympatric species, and the germplasm in each Germplasm Resources is carried out to purifying and preferred.
2. the building method of radix pseudostellariae Germplasm Resources according to claim 1, is characterized in that, in the construction of described Germplasm Resources, except germplasm preserve railway carriage or compartment face vegetation because of plantation need destroyed, other vegetation all should be kept intact.
3. the building method of radix pseudostellariae Germplasm Resources according to claim 1, is characterized in that, in the selection of described germplasm, and 0.5% copper-bath seed soaking potato sterilization in 2 hours for balck shank.
4. the building method of radix pseudostellariae Germplasm Resources according to claim 1, is characterized in that, in the selection of described germplasm, radix pseudostellariae ploughs under soil layer by table soil after planting and being made into 800 times of liquid spray furrow faces with 0.5 kilogram of 50% phoxim emulsifiable concentrate in first 15 days.
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| CN106665333A (en) * | 2017-01-25 | 2017-05-17 | 宁德师范学院 | Artificial cross pollination method of radix pseudostellariae |
| CN108684477A (en) * | 2017-02-28 | 2018-10-23 | 攸县炬丰中草药种植专业合作社 | A kind of implantation methods of radix pseudostellariae |
| CN112005823A (en) * | 2020-07-27 | 2020-12-01 | 重庆市农业科学院 | Ecological planting method for strawberry variety resource preservation |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105493836A (en) * | 2015-12-11 | 2016-04-20 | 贵州省黔康生态科技农业发展有限责任公司 | Method for preventing degeneration of Pseudostellaria heterophylla variety |
| CN106665333A (en) * | 2017-01-25 | 2017-05-17 | 宁德师范学院 | Artificial cross pollination method of radix pseudostellariae |
| CN106665333B (en) * | 2017-01-25 | 2018-11-13 | 宁德师范学院 | A kind of radix pseudostellariae artificial hybridization pollinating method |
| CN108684477A (en) * | 2017-02-28 | 2018-10-23 | 攸县炬丰中草药种植专业合作社 | A kind of implantation methods of radix pseudostellariae |
| CN112005823A (en) * | 2020-07-27 | 2020-12-01 | 重庆市农业科学院 | Ecological planting method for strawberry variety resource preservation |
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Application publication date: 20140806 |