CN102612962A - Method for identifying resistance of sweet potatoes in seedling stage to black spot - Google Patents
Method for identifying resistance of sweet potatoes in seedling stage to black spot Download PDFInfo
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- CN102612962A CN102612962A CN2012100950522A CN201210095052A CN102612962A CN 102612962 A CN102612962 A CN 102612962A CN 2012100950522 A CN2012100950522 A CN 2012100950522A CN 201210095052 A CN201210095052 A CN 201210095052A CN 102612962 A CN102612962 A CN 102612962A
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Abstract
The invention relates to a method for identifying resistance of crops to plant diseases and insect pests, in particular to a method for identifying resistance of sweet potatoes in a seedling stage to black spot. The method is scientific, and easy to implement, and has low requirements on experimental conditions. The method comprises the following steps of: taking shoot-tip seedlings of sweet potatoes which grow consistently in a seedling bed or a field, cutting off the lowest 2-3 leaves, cleaning by using a disinfectant fluid, putting into 1*10<4>/ml black spot spore suspension which is prepared by using sterile water in advance, culturing at room temperature of between 25 and 27DEG C for 6 days, investigating harm degrees in which experimental sweet potato seedlings are infected by the black spot, calculating each repeated average harm level, and judging the infection resistance of the experimental sweet potato seedlings to the black spot according to the harm level.
Description
Technical field
The present invention relates to a kind of corps diseases resistance authentication method, is exactly a kind of sweet potato black rot resistance in seedling stage authentication method specifically, belongs to the agriculture plant protection field.
Background technology
Sweet potato black rot is the important fungal disease of a kind of main harm sweet potato root system and basal part of stem, and all there is generation in each sweet potato producing region, the world.Nineteen thirty-seven is imported China into, and existing 26 provinces, municipality directly under the Central Government, autonomous region take place should disease, all can cause harm at sweet potato seedbed, land for growing field crops and shelf time, and be one of the three big serious plant disease in northern China potato district.After the potato seedling is infected, produce the circle or the little blackspot of fusiformis of depression in stem, after enlarge gradually, around potato seedling base portion pin shape darkly, acrial part yellow leaf or the blackening of seedling base portion is rotted.Potato transplantation of seedlings land for growing field crops 1-2 is after week in spite of illness, and the radical leaves jaundice comes off, when seriously ill, and butt rot, only remaining fibrous vascular bundle, the potato seedling is withered.After shelf time potato piece was injured, scab just was the black point, was expanded into circle or fusiformis scab gradually, and the middle black thorn that produces is concurrent with other fungies and bacterium, is prone to cause rotten.The production loss that China every year causes because of this disease is about 10%.
Research shows that the seed selection of disease-resistant variety is the main means of this disease of control with promoting.Therefore, estimating varietal resistance effectively, fast and accurately seems and is even more important.Many to the resistance identification research of sweet potato black rot is material with the potato piece, and the potato seedling is then less to the resistance identification research of black spot.(1990) such as Qiu Rui sickles adopt potato climing entry needle inoculation in land for growing field crops to identify, but because the difference of the climing growing way of potato and wood fibre cellulose content between different cultivars, are prone to cause result's error.
Summary of the invention
The objective of the invention is for overcoming the weak point of above-mentioned prior art, a kind of sweet potato black rot resistance in seedling stage authentication method is provided.After this method was removed the bottom petiole through the consistent top seedling of sweet potato that will grow, it was 1 * 10 that immersion prepares concentration
4In the black spot spore suspension of individual/ml; Can tell the steps such as anti-sense degree of different cultivars potato seedling after six days fast to black spot; Can confirm the resistance level of potato seedling fast and accurately, have easyly, be prone to learn black spot; Advantage such as time saving and energy saving is for sweet potato breeding for disease resistance screening bulk materials provides technical support.
The present invention realizes with following technical scheme: a kind of sweet potato black rot resistance in seedling stage authentication method is characterized in that: carry out as follows:
(1) germ separates and cultivates: gather morbidity potato piece from the field, isolate alternaria, on potato dextrose agar, cultivated 7 days with the fungi separating method, and subsequent use;
(2) the potato seedling of participating in the experiment is prepared: from the seedbed or 6 consistent top seedling of sweet potato of growth of field clip; Looking potato seedling internode length is advisable with 5-6 internode; Cut bottom two blades from petiole base after taking back laboratory, clean up potato seedling basal part of stem with aqua sterilisa, subsequent use;
(3) preparation of inoculation bacterium liquid: will be in the conidium that grows on the potato dextrose agar with under the aseptic water washing, using sterile water to be mixed with concentration is 1 * 10
4The spore suspension of individual/ml is subsequent use;
(4) inoculation is identified: the spore suspension for preparing is divided in the aseptic triangular flask that capacity is 250ml; To supply examination potato seedling to put down gently in the triangular flask then; 3 of every kinds are 3 repetitions, cultivate down at 25-27 ℃; Establish blank sterile water simultaneously for contrast, add with sterile water after the consumption of triangular flask culture fluid;
(5) research and appraisal: 6 days " Invest, Then Investigate " potato seedling waters surface are with lower part black spot incidence, and according to occurring degree the potato seedling are divided into 0-4 level totally 5 grades, according to be injured grade with kind to the resistance of black spot be divided into disease-resistant, in anti-and susceptible three ranks;
(6) the potato seedling grade scale of being injured: 0 grade: can't see illness; 1 grade: have fragmentary minor illness spot to occur, scab number≤20; 2 grades: scab is bigger, and scab counts 20 of ﹥, but not in flakes; 3 grades: scab is more, and spot is linked to be sheet, length≤2cm; 4 grades: scab in flakes, and length ﹥ 2cm in flakes;
(7) average onset grade=(repeating I rank+repetition II rank+repetition III rank)/3; Disease-resistant rank is confirmed: disease-resistant: the rank of being injured≤1.0; In anti-: the 1.0＜rank of being injured＜3.0; Susceptible: the rank of being injured>=3.0.
Advantage of the present invention is: this method is a material with seedbed or land for growing field crops potato seedling stem apex, and the indoor inoculation alternaria is identified disease resistance, can show the resistance level of different cultivars potato seedling to sweet potato black rot accurately, fast, intuitively.This method more approaches the actual conditions that the field sweet potato's seedlings infects black spot, simple to operate, easy learning, and less demanding to experimental facilities, each breeding units all can be carried out evaluation work.This authentication method can be identified for the sweet potato breeding for disease resistance in earlier stage provides technical support, rejects the part susceptible variety, for the later stage screening varieties alleviates human and material resources.Simultaneously, be expert evidence with seedbed or land for growing field crops potato seedling stem apex, can reduce the otherness of drawing materials to greatest extent, reduce experimental error.Indoor disinfection inoculation is easy to experimental condition control, has also strengthened result's accuracy.Promoting the use of of this method is for screening disease-resistant variety and instructing the significant and realistic function of sweet potato breeding for disease resistance.
Embodiment
Below in conjunction with embodiment the present invention is described further:
Embodiment,
Sweet potato black rot resistance in seedling stage authentication method, we carry out black spot resistance approval test to the potato seedling of 13 sweet potato varieties that gather in the seedbed, carry out as follows:
(1) germ separates and cultivates: gather morbidity potato piece from the field, isolate alternaria, on potato dextrose agar, cultivated 7 days with the fungi separating method, and subsequent use;
(2) the potato seedling of participating in the experiment is prepared: gather No. hundred, North China 52-45, Nanjing 92, Xu's potato 18, Zhejiang Xu No. 1, Xu 273, Ji farming 22, Yantai 252, Xu 02-9-11, Xu 02-16-17, peaceful 55-1, Xu 22-1, Xu 25-2, triumph totally 13 sweet potato varieties (being) potato seedling from the seedbed; Every kind is 6 consistent top seedling of sweet potato of growth of clip respectively; Looking potato seedling internode length is advisable with 5-6 internode; Cut bottom 2-3 sheet blade from petiole base after taking back laboratory; Clean up potato seedling basal part of stem with aqua sterilisa, subsequent use;
(3) preparation of inoculation bacterium liquid: will be in the black spot conidium that grows on the potato dextrose agar with under the aseptic water washing, using sterile water to be mixed with concentration is 1 * 10
4The spore suspension of individual/ml is subsequent use;
(4) inoculation is identified: the spore suspension for preparing is divided in the aseptic triangular flask that capacity is 250ml; To supply examination potato seedling to put down gently in the triangular flask then; 3 of every kinds are 3 repetitions, cultivate down at 25-27 ℃; Establish blank sterile water simultaneously for contrast, fill it up with sterile water after the culture fluid consumption;
(5) research and appraisal as a result: the " Invest, Then Investigate " potato seedling water surface was with lower part black spot incidence in 6 days; And based on occurring degree the potato seedling is divided into 0-4 level totally 5 grades, based on be injured grade with kind to the resistance of black spot be divided into disease-resistant, in anti-and susceptible three ranks;
The potato seedling grade scale of being injured:
0 grade: can't see illness;
1 grade: have fragmentary minor illness spot to occur, scab number≤20;
2 grades: scab is bigger, and scab counts 20 of ﹥, but not in flakes;
3 grades: scab is more, and spot is linked to be sheet, length≤2cm;
4 and: scab in flakes, and length ﹥ 2cm in flakes;
Average onset grade=(repeating I rank+repetition II rank+repetition III rank)/3;
Disease-resistant rank is confirmed: disease-resistant: the rank of being injured≤1.0; In anti-: the 1.0＜rank of being injured＜3.0; Susceptible: the rank of being injured>=3.0.
The test qualification result is seen table 1:
Table 1 sweet potato black spot in seedling stage resistance qualification result
Conclusion: can find out that from table 1 in 14 sweet potato varieties being identified, Xu 02-9-11, Nanjing 92, North China 52-45 and Xu's potato 18 totally 4 kinds (being) potato seedling are " disease-resistant " to the resistance rank of black spot; Xu potato 25-2, Xu's potato 22-1, Zhejiang Xu No. 1, Xu 273 and Xu 02-16-17 totally 5 kinds (being) potato seedling are " in anti-" to the resistance rank of black spot; Ji farming 22, No. hundred, triumph, peaceful 55-1 and Yantai 252 the resistance rank of totally 4 article potato seed seedling black spots are " susceptible ".
Claims (1)
1. sweet potato black rot resistance in seedling stage authentication method is characterized in that: carry out as follows:
(1) germ separates and cultivates: gather morbidity potato piece from the field, isolate alternaria, on potato dextrose agar, cultivated 7 days with the fungi separating method, and subsequent use;
(2) the potato seedling of participating in the experiment is prepared: from the seedbed or 6 consistent top seedling of sweet potato of growth of field clip; Looking potato seedling internode length is advisable with 5-6 internode; Cut bottom two blades from petiole base after taking back laboratory, clean up potato seedling basal part of stem with aqua sterilisa, subsequent use;
(3) preparation of inoculation bacterium liquid: will be in the conidium that grows on the potato dextrose agar with under the aseptic water washing, using sterile water to be mixed with concentration is 1 * 10
4The spore suspension of individual/ml is subsequent use;
(4) inoculation is identified: the spore suspension for preparing is divided in the aseptic triangular flask that capacity is 250ml; To supply examination potato seedling to put down gently in the triangular flask then; 3 of every kinds are 3 repetitions, cultivate down at 25-27 ℃; Establish blank sterile water simultaneously for contrast, add with sterile water after the consumption of triangular flask culture fluid;
(5) research and appraisal: 6 days " Invest, Then Investigate " potato seedling waters surface are with lower part black spot incidence, and according to occurring degree the potato seedling are divided into 0-4 level totally 5 grades, according to be injured grade with kind to the resistance of black spot be divided into disease-resistant, in anti-and susceptible three ranks;
(6) the potato seedling grade scale of being injured: 0 grade: can't see illness; 1 grade: have fragmentary minor illness spot to occur, scab number≤20; 2 grades: scab is bigger, and scab counts 20 of ﹥, but not in flakes; 3 grades: scab is more, and spot is linked to be sheet, length≤2cm; 4 grades: scab in flakes, and length ﹥ 2cm in flakes;
(7) average onset grade=(repeating I rank+repetition II rank+repetition III rank)/3; Disease-resistant rank is confirmed: disease-resistant: the rank of being injured≤1.0; In anti-: the 1.0＜rank of being injured＜3.0; Susceptible: the rank of being injured>=3.0.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010126A (en) * | 2015-07-06 | 2015-11-04 | 山东省农业科学院作物研究所 | Fast breeding method for black spot resistant sweet potato variety |
| CN106031352A (en) * | 2015-03-11 | 2016-10-19 | 李保华 | A method for evaluating the prevention and control effects of fungicides on apple floral organ-infected diseases |
| CN107258417A (en) * | 2016-04-06 | 2017-10-20 | 万素梅 | The occurrence regularity of jujube alternaria |
| CN109392646A (en) * | 2018-11-23 | 2019-03-01 | 云南省烟草农业科学研究院 | A kind of investigation method of tobacco black shank resistance |
| CN109507368A (en) * | 2018-11-16 | 2019-03-22 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of accurate Resistance Identification method of seedling stage sweet potato black rot |
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2012
- 2012-04-01 CN CN 201210095052 patent/CN102612962B/en not_active Expired - Fee Related
Non-Patent Citations (4)
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| 李淑菊等: "黄瓜黑斑病苗期抗病性鉴定方法及品种抗病性评价", 《中国蔬菜》 * |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106031352A (en) * | 2015-03-11 | 2016-10-19 | 李保华 | A method for evaluating the prevention and control effects of fungicides on apple floral organ-infected diseases |
| CN106031352B (en) * | 2015-03-11 | 2018-12-04 | 李保华 | Method for evaluating control effect of bactericide on apple floral organ infected diseases |
| CN105010126A (en) * | 2015-07-06 | 2015-11-04 | 山东省农业科学院作物研究所 | Fast breeding method for black spot resistant sweet potato variety |
| CN105010126B (en) * | 2015-07-06 | 2017-09-26 | 山东省农业科学院作物研究所 | A kind of quick breeding method of anti-black spot sweet potato variety |
| CN107258417A (en) * | 2016-04-06 | 2017-10-20 | 万素梅 | The occurrence regularity of jujube alternaria |
| CN109507368A (en) * | 2018-11-16 | 2019-03-22 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of accurate Resistance Identification method of seedling stage sweet potato black rot |
| CN109392646A (en) * | 2018-11-23 | 2019-03-01 | 云南省烟草农业科学研究院 | A kind of investigation method of tobacco black shank resistance |
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