CN102715009B - Pistacia chinensis bunge cutting propagation method under the condition of high-concentration CO2 - Google Patents
Pistacia chinensis bunge cutting propagation method under the condition of high-concentration CO2 Download PDFInfo
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- CN102715009B CN102715009B CN201210239720.4A CN201210239720A CN102715009B CN 102715009 B CN102715009 B CN 102715009B CN 201210239720 A CN201210239720 A CN 201210239720A CN 102715009 B CN102715009 B CN 102715009B
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Abstract
The invention discloses a pistacia chinensis bunge cutting propagation method under the condition of high-concentration CO2, which comprises the following steps: 1) dipping selected semi-ligneous pistacia chinensis bunge cuttings in plant growth regulator solution; and 2) cutting the treated cuttings in matrix, and then putting the matrix in an incubator with the air CO2 concentration of 750+/-50mu mol.mol<-1>-3000+/-50mu mol.mol<-1> for culture. In the pistacia chinensis bunge cutting propagation method, firstly the pistacia chinensis bunge semi-ligneous cuttings are dipped in a plant growth regulator, and then specific matrix, CO2 concentration, illumination intensity, air humidity, temperature and other culture conditions are selected for cutting propagation so as to greatly improve the cutting propagation survival rate and seedling quality of pistacia chinensis bunge.
Description
Technical field
The present invention relates to a kind of cuttage breeding method, espespecially a kind of Chinese pistache cuttage breeding method.
Background technology
Pistache Anacardiaceae pistache, be a kind of important energy tree species, its oil content nearly 40%, Chinese pistache not only grease yield is high, and material, wood property are good, is good in material and Ecological Afforestation seeds, therefore, Chinese pistache has vast potential for future development.At present; Chinese pistache is grown seedlings and is mainly passed through seed propagation; but produce seedling by sowing and be difficult to keep the good habit of maternal plant; be unfavorable for the breeding breeding; and tissue culture technique rests on the laboratory test stage, do not move towards yet production application, cottage propagation is because the branch tannin content is high; the difficulty of taking root, these have seriously restricted the scale of Chinese pistache breeding nursery stock production.Therefore, developing efficient Chinese pistache vegetative propagation technique is the urgent problem faced in current production.
Summary of the invention
The problem existed for prior art, the object of the present invention is to provide the Chinese pistache cuttage breeding method that a kind of survival rate is high.
For achieving the above object, high concentration CO of the present invention
2chinese pistache cuttage breeding method under condition comprises: 1) in plant growth regulator solution, dip the semi-lignified Chinese pistache of choosing and transplant a cutting; 2) by above-mentioned treated cutting cuttage in matrix, then put into air CO
2concentration is 750 ± 50 μ molmol
-1～3000 ± 50 μ molmol
-1incubator in cultivated.
Further, the described Chinese pistache of choosing is transplanted a cutting as well-developed Chinese pistache semi-lignified spray stem section.
Further, described matrix is a kind of in vermiculite, perlite and coarse sand.
Further, described plant growth regulator is 200mg/L IBA, and dipping the cutting time is 5s-5min.
Further, the cutting time of dipping of described 200mg/L IBA is 5min, and described matrix is vermiculite.
Further, in described incubator, intensity of illumination is 37.5 μ molm
-2s
-1～112.5 μ molm
-2s
-1.
Further, described incubator condition of culture is: CO
2concentration 1450～1550 μ molmol
-1, intensity of illumination 62.5～87.5 μ molm
-2s
-1, 25～30 ℃ of air humiditys 80～90%, temperature.
Chinese pistache high concentration CO of the present invention
2at first cuttage breeding method carries out plant growth regulator and dips by Chinese pistache is transplanted a cutting, then by choosing specific matrix and CO
2the condition of culture such as concentration, intensity of illumination, air humidity, temperature carry out cottage propagation, make the Chinese pistache cuttage survival rate reach 94.2%, 19.7 of cuttage seeding radicals, and the long average out to 15.5cm of root, gained cuttage seeding root growth is good, the nursery stock stalwartness.
Embodiment
Chinese pistache high concentration CO of the present invention
2cuttage breeding method comprises: 1) clip Chinese pistache semi-lignified spray stem section, in plant growth regulator solution, dip; 2) by above-mentioned treated cutting cuttage in the blake bottle that matrix is housed or cuttage seeding dish, then put into high concentration CO
2incubator in cultivated.Wherein, described matrix is a kind of in vermiculite, perlite and coarse sand; Described plant growth regulator is 200mg/L IBA, and dipping the cutting time is 5s-5min; Described high concentration CO
2be 750 ± 50 μ molmol
-1～3000 ± 50 μ molmol
-1; In described incubator, intensity of illumination is 37.5 μ molm
-2s
-1～112.5 μ molm
-2s
-1, air humidity 80～90%, 25～30 ℃ of temperature.In said method of the present invention, optimum condition is: the cutting time of dipping of described 200mg/L IBA is 5min, and described matrix is vermiculite; Described incubator condition of culture is: CO
2concentration 1450～1550 μ molmol
-1, intensity of illumination 62.5～87.5 μ molm
-2s
-1, 25～30 ℃ of air humiditys 80～90%, temperature; The described Chinese pistache of choosing is transplanted a cutting as well-developed Chinese pistache semi-lignified spray, is cut into long 6-8cm, and diameter is 0.5-1cm, 2-3 axillalry bud, the stem section of reservation 7-10 sheet leaf.
Below verify by experiment Chinese pistache high concentration CO of the present invention
2the feasibility of cuttage breeding method.
1. test method of the present invention
Chinese pistache of the present invention is transplanted a cutting and is taken from well-developed Chinese pistache in proving ground, Beijing Agricultural College Technology Park (Pistacia chinensis Bunge) semi-lignified spray stem section, its specification is long 6-8cm, diameter is 0.5-1cm, the top tender leaf of 2-3 axillalry bud shows red semi-lignified branch, and blade retains 7-10.
1.1. test one for the test of cuttage primary condition, adopt 3 factor 3 horizontal quadrature design L
9(3
4).A is plant growth regulator treatment: A
1for IBA200mg/L dips cutting 5min, A
2for 200mg/LIBA dips cutting 2min, A
3for 200mg/L IBA dips cutting 5s; B is matrix: B
1for vermiculite, B
2for perlite, B
3for coarse sand; C is that sucrose solution is processed: C
1for 0g/L sucrose solution, C
2for 5g/L sucrose solution, C
3for the 15g/L sucrose solution.Each processes 8 bottles, repeats 3 times.It is the upper footpath of 5cm * 6cm * 11.5cm(×Xia footpath * height that culture vessel is selected specification), every bottle holds matrix 120g, matrix sterilizing 1h, and seal after adding nutrient solution 30ml, and then by blake bottle sterilizing 1h in high-pressure sterilizing pot.First will transplant a cutting during cuttage and clean with dish detergent, then with alcohol by the socket 5s that sterilizes rapidly, then scrub 6 times with sterile water, cuttage is in the experimental group blake bottle, the degree of depth of cuttage is about 1/3 of cutting.
Above test condition of culture is: CO
2concentration 1450～1550 μ molmol
-1, intensity of illumination 62.5～87.5 μ molm
-2s
-1, 25～30 ℃ of air humiditys 80～90%, temperature.
1.2 test two for the strength test of Chinese pistache cuttage optimum illumination, determine the best Chinese pistache cuttage primary condition filtered out by orthogonal experiment, specific operation process is with 1.1.Establish altogether 4 intensities of illumination, be respectively light quantum current density 37.5 μ molm
-2s
-1, 62.5 μ mo lm
-2s
-1, 87.5 μ molm
-2s
-1, 112.5 μ molm
-2s
-1.Each processes 8 bottles of cuttages, and cuttage is 32 bottles altogether, repeats 3 times.Every 3d waters once, after 45 days, statistical analysis is investigated and measure and done to the indexs such as root morphology index, root activity, Photosynthetic Index.
1.3 test three for the best CO of Chinese pistache cuttage
2the concentration test.Determine the best Chinese pistache cuttage primary condition filtered out by orthogonal experiment, specific operation process is with 1.1.Establish altogether 3 CO
2concentration is processed, and is respectively 750 ± 50 μ molmol
-1, 1500 ± 50 μ molmol
-1, 3000 ± 50 μ molmol
-1.Each processes 8 bottles of cuttages, and cuttage is 24 bottles altogether, repeats 3 times.Every 3d waters once, and intensity of illumination is 87.5 μ molmol
-1.After 45 days, statistical analysis is investigated and measured and done to the indexs such as root morphology index, root activity, Photosynthetic Index.
2. index determining
(1) mensuration of root morphology index: only investigate the Chinese pistache cuttage seeding rooting rate, take root the number and root long.Rooting rate=cuttage root-taking number/cuttage sum * 100% wherein, the number=main root of taking root+lateral root (counting when wherein lateral root is from main root 0.5cm and d>=0.1cm), the main root length (cm) of root length=cuttage seeding; (2) mensuration of root activity is with reference to the method for Hao Zaibin; (3) mensuration of Photosynthetic Index: the Chinese pistache cuttage seeding is measured after cultivating 45d, uses portable photosynthetic instrument (LI-6400, LI-COR Inc, USA) to coordinate self-control leaf chamber to measure the Photosynthetic Indexes such as the photosynthetic rate of cuttage seeding, stomatal conductance, transpiration rate.
Adopt Microsoft Excel, DPS7.05 data analysis system to carry out variance analysis, correlation analysis etc. to the experimental observation data information.
3. results and analysis
3.1 determining of cuttage primary condition
Can find out from table 1 and table 2, rooting rate, several influence factor of taking root are followed successively by B, A, C from big to small, be Main Factors on the A that affects of root length, and B takes second place, the C minimum.Obviously, the HORMONE TREATMENT of matrix and cutting is taken root remarkable impact is arranged the Chinese pistache cuttage seeding, and different sucrose solution processing impact is less.Known by further multiple comparison analyse, dip cutting 5min(A with IBA200mg/L
1) carrying out cuttage, the rooting rate of Chinese pistache cuttage seeding, the number of taking root, root reach maximum, are respectively 82.9%, 16.2, and 13.3cm, be significantly higher than other levels.With vermiculite (B
1) significantly being better than coarse sand and perlite as matrix, the rooting rate of Chinese pistache, the number of taking root, root length all reach maximum, are respectively 83.0%, 16.6,10.4cm.
Table 1 Chinese pistache Orthogonal Experiment and Design and interpretation of result
Between each level of the table 2 test processing factor, significance of difference multiple ratio
The combination of the different factors of analysis-by-synthesis under varying level, the best of breed that sums up the Chinese pistache cottage propagation is A
1b
1be that IBA200mg/L dips cutting 5min+ vermiculite.
3.2 intensity of illumination is taken root and photosynthetic impact on the Chinese pistache cuttage seeding
As can be seen from Table 3, different illumination intensity does not make significant difference to Chinese pistache cuttage seeding rooting rate, but radical, root length and root activity are had to remarkable impact.Along with the increase of intensity of illumination, cuttage seeding radical, root length and root activity are increase trend, but work as intensity of illumination, are greater than 87.5 μ molm
-2s
-1after, each index changes not obvious.
The impact of the different light quantum current densitys of table 3 on Chinese pistache cuttage seeding rooting rate, root amount and root activity
Different illumination intensity shows (in Table 4) to the impact of Chinese pistache cuttage seeding net photosynthetic rate, stomatal conductance and transpiration rate, light quantum current density 37.5 μ molm
-2s
-1with 62.5 μ molm
-2s
-1the photosynthetic rate difference little, but light quantum current density 87.5 μ molm
-2s
-1with 112.5 μ molm
-2s
-1the time photosynthetic rate significantly reduce, this explanation intensity of illumination surpasses 62.5 μ molm
-2s
-1the time, the photosynthetic activity of Chinese pistache cuttage seeding significantly reduces along with the enhancing of intensity of illumination.The stomatal conductance of Chinese pistache cuttage seeding and transpiration rate are with light quantum current density 87.5 μ molm
-2s
-1the highest, be respectively 0.24mmolm
-2s
-1, 7.71mmolm
-2s
-1, other are processed without significant difference.Result shows, the Chinese pistache cuttage seeding surpasses 87.5 μ molm in intensity of illumination
-2s
-1the time, its physiologically active is subject to certain restrictions, therefore suitable light quantum current density is 62.5～87.5 μ molm
-2s
-1.
The impact of table 4 intensity of illumination on Chinese pistache cuttage seeding Photosynthetic Index
3.3 high concentration CO
2the Chinese pistache cuttage seeding is taken root in photosynthetic impact
Table 5 shows, high concentration CO
2chinese pistache cuttage root-taking rate and root growth are had to extremely significantly facilitation.CO
2concentration is 1500 ± 50 μ molmol
-1, the rooting rate of Chinese pistache cuttage seeding, the number of taking root, root length and improving activity of root system are all maximum, are significantly higher than other processing, work as CO
2concentration is 3000 ± 50 μ molmo1
-1the time, Chinese pistache cuttage seeding improving activity of root system and root amount descend on the contrary, are attended by rotten, the withered even phenomena of mortality of plant.
Table 5 high concentration CO
2impact on Chinese pistache cuttage seeding rooting rate, root amount and root activity
Different CO
2the variation of Chinese pistache cuttage seeding photosynthetic rate, stomatal conductance and transpiration rate under concentration is in Table 6, different CO
2all there is significant difference in the Photosynthetic Index that concentration is processed Chinese pistache, works as CO
2concentration is 1500 ± 50 μ molmol
-1the time, photosynthetic rate and transpiration rate are the highest, reach respectively 12.78 ± 1.12 μ molmo1
-1with 6.08 ± 2.34 μ molmol
-1, and work as CO2 concentration, continue to be increased to 3000 ± 50 μ molmol
-1photosynthetic Index descends on the contrary, negative effect occurs.
Table 6 high concentration CO
2chinese pistache cuttage seeding Photosynthetic Index is affected
4 conclusions
This method selects the Chinese pistache of semi-lignified to give birth to then spray as cutting, and the IBA plant growth regulator treatment cutting 5min by 200mg/L concentration, adopt vermiculite as matrix, at condition of culture, is: CO
2concentration 1450～1550umolmol
-1, intensity of illumination 62.5～87.5umo lm
-2s
-1the time, 25～30 ℃ of air humiditys 80～90%, temperature, the rooting of cuttings rate is up to 94.2%, 19.7 of radicals, the long average out to 15.5cm of root, the cuttage seeding root system development is good, robust growth.
In the tradition cuttage technique, seldom add sucrose solution, and add the differentiation of the favourable plant tissue of sugar and take root when tissue is cultivated.This test finds, do not add that sucrose solution is processed and high dense sucrose solution (15g/L) is processed Chinese pistache rooting of cuttings rate, the number of taking root, root longly all without obvious facilitation, and the Chinese pistache of high concentration sucrose solution-treated cutting on the contrary, with a little bad root phenomenon.Therefore, cuttage time suggestion does not add saccharose treatment.
Illumination is a key factor that affects cuttage and seedling culture.This result of the test shows, different illumination intensity does not make significant difference to Chinese pistache cuttage seeding rooting rate, but along with the increase of intensity of illumination, and the cuttage seeding radical, root is long and root activity is remarkable increase trend, when intensity of illumination is 87.5 μ molm
-2s
-1the time, each index reaches maximum, and when light intensity continues to increase, each index changes not obvious.Working as intensity of illumination in this test is 62.5 μ molm
-2s
-1the time, the photosynthetic rate of cuttage seeding reaches maximum, then improves intensity of illumination, and photosynthetic rate descends on the contrary, and transpiration rate increases.Obviously, within the specific limits, intensity of illumination is higher, and the photosynthetic rate of cuttage seeding is just larger, and synthetic organic substance is many, can promote the cuttage seeding root growth, but, when intensity of illumination is excessive, can cause on the contrary the decline of photosynthetic rate, unfavorable to the cuttage seeding root growth.Therefore, the Chinese pistache cuttage seeding being cultivated to optimum illumination intensity is 62.5～87.5umolm
-2s
-1.
CO
2concentration is an important indicator that affects photosynthesis of plant, due to CO in atmosphere
2concentration is on the low side, particularly in growth chamber and greenhouse, due to the CO in a large amount of consumed cabin air of photosynthesis of plant
2, make the CO in environment
2concentration is lower than in atmosphere, thereby growing of plant produced to the downward effect.First passage of the present invention enriches high concentration CO
2observe the situation of growing of Chinese pistache cuttage seeding.Result of the test shows, high CO
2under concentration, Chinese pistache rooting rate, root amount and root activity all extremely significantly increase, and show to increase CO
2concentration is very favourable to the root growth and development of Chinese pistache cuttage seeding; CO in the while environment
2concentration increases, and the Photosynthetic Index of Chinese pistache cuttage seeding etc. significantly increase, and high photosynthetic rate can increase the synthetic of carbohydrate, promotes the growth of cuttage seeding branches and leaves and growing of root system; But work as CO
2concentration is increased to 3000 μ molmol
-1, Chinese pistache cuttage seeding photosynthesis descends, and rooting rate, root amount and root activity also significantly descend, and follow the root system putrefactive phenomenon, and this may be because the interior CO of incubator
2excessive concentration, cause there is no enough oxygen supplies, when root system, during in anoxia condition, causes anaerobic respiration, and mesostate causes the physiology injury of plant as ethanol, acetaldehyde, acetic acid etc., on the other hand, and too high CO
2concentration also can cause inhibitory action to the photosynthesis of cuttage seeding, thereby has influence on growing of root system.Therefore, the Chinese pistache cuttage seeding is cultivated to optimum air CO
2concentration is 1450～1550 μ molmol
-1.
Claims (7)
1. high concentration CO
2chinese pistache cuttage breeding method under condition, is characterized in that, comprising: 1) in plant growth regulator solution, dip the semi-lignified Chinese pistache of choosing and transplant a cutting; 2) by above-mentioned treated cutting cuttage in matrix, then put into air CO
2concentration is 750 ± 50 μ molmol
-1～3000 ± 50 μ molmol
-1incubator in cultivated.
2. high concentration CO as claimed in claim 1
2chinese pistache cuttage breeding method under condition, is characterized in that, the described Chinese pistache of choosing is transplanted a cutting as well-developed Chinese pistache semi-lignified spray stem section.
3. high concentration CO as claimed in claim 1
2chinese pistache cuttage breeding method under condition, is characterized in that, described matrix is a kind of in vermiculite, perlite and coarse sand.
4. high concentration CO as claimed in claim 1
2chinese pistache cuttage breeding method under condition is characterized in that described plant growth regulator is 200mg/L IBA, and dipping the cutting time is 5s-5min.
5. high concentration CO as claimed in claim 4
2chinese pistache cuttage breeding method under condition, is characterized in that, the cutting time of dipping of described 200mg/LIBA is 5min, and described matrix is vermiculite.
6. described high concentration CO as arbitrary as claim 1-5
2chinese pistache cuttage breeding method under condition, is characterized in that, in described incubator, intensity of illumination is 37.5 μ molm
-2s
-1～112.5 μ molm
-2s
-1.
7. high concentration CO as claimed in claim 6
2chinese pistache cuttage breeding method under condition, is characterized in that, described incubator condition of culture is: CO
2concentration 1450～1550 μ molmol
-1, intensity of illumination 62.5～87.5 μ molm
-2s
-1, 25～30 ℃ of air humiditys 80～90%, temperature.
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|---|---|---|---|
| CN201210239720.4A CN102715009B (en) | 2012-07-11 | 2012-07-11 | Pistacia chinensis bunge cutting propagation method under the condition of high-concentration CO2 |
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|---|---|---|---|
| CN201210239720.4A CN102715009B (en) | 2012-07-11 | 2012-07-11 | Pistacia chinensis bunge cutting propagation method under the condition of high-concentration CO2 |
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| CN105519421B (en) * | 2016-01-11 | 2019-03-15 | 四川七彩林业开发有限公司 | A kind of red autumnal leaves Chinese pistache training tissue culture seedling matrix and its hardening off method |
| CN106857140A (en) * | 2017-01-18 | 2017-06-20 | 河南省林业科学研究院 | The cuttage breeding method of poppyhead stool fruit |
| CN111345163A (en) * | 2020-03-18 | 2020-06-30 | 吉林省林业科学研究院 | Rapid rooting method for tilia amurensis cutting slips |
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Non-Patent Citations (4)
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| 何敬房等.黄连木茎段启动与增殖培养中防褐化技术研究.《河北林果研究》.2011,第26卷(第3期), |
| 杨镇等.黄连木嫩枝扦插育苗研究.《河北林果研究》.1997,第12卷(第1期), |
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