CN106661612A - 制备用于测序的核酸的方法 - Google Patents
制备用于测序的核酸的方法 Download PDFInfo
- Publication number
- CN106661612A CN106661612A CN201580016908.4A CN201580016908A CN106661612A CN 106661612 A CN106661612 A CN 106661612A CN 201580016908 A CN201580016908 A CN 201580016908A CN 106661612 A CN106661612 A CN 106661612A
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- China
- Prior art keywords
- target
- primer
- nucleic acid
- sequence
- specific
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461931959P | 2014-01-27 | 2014-01-27 | |
| US61/931,959 | 2014-01-27 | ||
| PCT/US2015/012891 WO2015112974A1 (en) | 2014-01-27 | 2015-01-26 | Methods of preparing nucleic acids for sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106661612A true CN106661612A (zh) | 2017-05-10 |
Family
ID=53678460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580016908.4A Pending CN106661612A (zh) | 2014-01-27 | 2015-01-26 | 制备用于测序的核酸的方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (4) | US10450597B2 (https=) |
| EP (2) | EP3099820A4 (https=) |
| JP (2) | JP7016215B2 (https=) |
| CN (1) | CN106661612A (https=) |
| AU (2) | AU2015209079B2 (https=) |
| WO (1) | WO2015112974A1 (https=) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108220392A (zh) * | 2017-08-01 | 2018-06-29 | 深圳恒特基因有限公司 | 富集和确定靶核苷酸序列的方法 |
| WO2019090482A1 (zh) * | 2017-11-07 | 2019-05-16 | 北京优乐复生科技有限责任公司 | 一种第二代高通量测序文库构建方法 |
| CN110241209A (zh) * | 2018-03-09 | 2019-09-17 | 林云富 | 一种引物、试剂盒及用途 |
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