CN106638339B - Algae blanket and preparation method thereof - Google Patents
Algae blanket and preparation method thereof Download PDFInfo
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- CN106638339B CN106638339B CN201710034498.7A CN201710034498A CN106638339B CN 106638339 B CN106638339 B CN 106638339B CN 201710034498 A CN201710034498 A CN 201710034498A CN 106638339 B CN106638339 B CN 106638339B
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Abstract
The invention relates to the field of environmental protection, and particularly relates to an algae blanket and a preparation method thereof. The algae blanket comprises an upper layer protection fiber fabric layer, an algae seed layer, a matrix layer, a shaping support net and a bottom layer bonding tape from top to bottom in sequence, wherein the upper layer protection fiber fabric layer, the shaping support net and the bottom layer bonding tape are sewn by sewing threads or bonded by modified starch glue, the matrix layer is bonded with the shaping support net, the algae seed layer is bonded with the matrix layer, the bottom layer bonding tape is a fiber fabric, and the two sides of the bottom layer bonding tape are respectively 2cm wider than the two sides of the shaping support net and serve as bonding tapes for bonding the algae blankets. A preparation method of an algal blanket comprises the following steps: (1) preparing a nutrient solution; (2) preparing a matrix; (3) preparing desert algae; (4) and (5) preparing the algal blanket.
Description
Technical Field
The invention relates to the field of environmental protection, and particularly relates to an algae blanket and a preparation method thereof.
Background
With the expansion of desertification and the emergence of a large number of bare lands formed by projects such as traffic, mining and construction, governments and subject experts in various countries seek better measures for treating drift sand lands, water and soil lost lands, poor lands and bare lands.
A large number of engineering, chemical, biological and comprehensive measures are adopted, but the engineering method has the problems of timeliness, difficult land recycling of chemical reagents and the like.
The biological comprehensive treatment is the most long-acting way for treating desertification and improving the environment, but the field is only limited to the optimized selection and the reasonable configuration of higher plants at present. Particularly in desert regions with the diameter of less than 200mm, the high-density large-area afforestation is carried out, although the ecological environment is improved in a short period, the artificial forest is quickly declined due to water shortage, and further ecological degradation and resource waste are caused. The water bearing capacity is the key of the vegetation type under different ecological conditions, and the water required by the microbial crusts is relatively less, so that the comprehensive coverage can be achieved, and the soil erosion amount can be reduced to zero. Desert regions consist of vascular bundles with a vegetation coverage of less than or equal to 30% (Townsend et al, 1986), while the cover of microbial crusts is greater than or equal to 70% (Belnap et al, 1994). Therefore, the research on the microbial crust in the desert area worldwide attracts much attention, and the research is extensively and deeply carried out from various aspects such as the composition, the structure succession, the action and the like of the microbial crust, and the research is tried to be applied to the aspects such as quicksand fixation and the like. Desert biological carpet engineering is proposed by Wen Jiangchun academia, and a 'desert algae fixing method for quicksand' is proposed by Huchun Xiang and the like (CN 1282511A): the screened algae seeds are directly sprayed on quicksand to produce artificial crust. Liu Yongding et al proposed "a method for biologically fixing quicksand and restoring desertified land (CN 1561698A)" combining desert algae with herbs and shrubs to fix quicksand. The above microbial skinning studies and applications are limited to the direct application of the skinning itself and its microorganisms. Whether in sand or open ground, the physical and chemical properties are harsh to the survival of the organism, and the microorganisms are difficult to grow by directly spraying or scattering the microorganisms on the ground surface. If the desert algae is combined with the growth substrate to form an artificial desert algae growth system, the desert algae grows in the adaptive substrate to form a growth microenvironment, and the survival, propagation and application capabilities of the desert algae are enhanced by artificially regulating the desert algae growth substrate, so that the efficiency of the desert algae in treating the ecological environment is improved. At present, vegetation blankets and biological blankets are applied to ecological construction, but the biological blankets are artificial biological crusts made of algae and mainly made of herbaceous plants, and the algae blankets have no patent documents so far.
Disclosure of Invention
The invention aims to provide an algae blanket and a preparation method thereof, aiming at the defects of the existing technology for applying algae to treat ecological environment, and provides a biological method for treating sandy land, inferior land and engineering bare land by applying modes such as full coverage or determinant laying of the algae blanket, which is suitable for treating bare land quickly and for a long term.
The technical scheme is as follows: an algae blanket comprises an upper layer protective fiber fabric layer, an algae seed layer, a matrix layer, a shaping support net and a bottom layer adhesive tape from top to bottom in sequence,
the upper layer protective fiber fabric layer, the shaping support net and the bottom layer bonding belt are sewn by adopting sewing threads or bonded by adopting modified starch glue,
the substrate layer is bonded with the shaping support net,
the algae seed layer is adhered to the matrix layer,
the bottom layer adhesive tape is a fiber fabric, and the two sides of the bottom layer adhesive tape are respectively wider than the two sides of the shaping support net by 2cm and are used as adhesive tapes for adhering the algae blankets to each other.
Further, the thickness of the upper protective fiber fabric layer is 0.2-0.3 mm.
Further, the thickness of the algae seed layer is 0.2-0.3 mm.
Further, the thickness of the substrate layer is 2.4-2.6 mm.
Further, the thickness of the shaping support net is 2.0-2.5 mm.
Further, the thickness of the bottom layer bonding tape is 0.2-0.3 mm.
A preparation method of the algae blanket comprises the following steps:
(1) preparation of nutrient solution
(11) Preparing nutrients:
weighing 0.8-2.5 parts by mass of sodium nitrate, 0.02-0.05 part by mass of dipotassium hydrogen phosphate, 0.06-0.09 part by mass of magnesium sulfate, 0.02-0.05 part by mass of calcium chloride, 0.004-0.008 part by mass of citric acid, 0.004-0.008 part by mass of ferric ammonium citrate, 0.001-0.003 part by mass of disodium ethylenediamine tetraacetic acid and 0.01-0.04 part by mass of sodium carbonate, and uniformly mixing the raw materials to obtain mixed powder for later use;
(12) preparing a solution of trace elements
2.86 parts by mass of H3BO31.81 parts by mass of MnCl2·H2O, 0.222 parts by mass of ZnSO4·7H2O, 0.079 parts by mass of CuSO4·5H2O, 0.390 part by mass of Na2MoO4·2H2O, 0.049 part by mass of Co (NO)3)2·6H2Adding O into a proper amount of water to prepare 1000 parts by mass of trace element solution,
(13) mixing the mixed powder obtained in the step (11) with the trace element solution obtained in the step (12) according to the mass ratio of (1.4-1.8): 1, preparing and uniformly mixing to prepare nutrient solution;
(2) preparation of the substrate
(21) Uniformly mixing 2.0 parts by mass of modified cellulose, 0.1 part by mass of modified starch, 20.0 parts by mass of cotton wool fibers, 1.0 part by mass of the nutrient solution obtained in the step (1), 10.0 parts by mass of attapulgite powder and 66.9 parts by mass of water to obtain a mixture;
(22) uniformly stirring the mixture obtained in the step (21) to prepare a colloid material, namely obtaining a matrix;
(3) preparation of desert algae
(31) Algal species acquisition
(311) Grinding the soil crust with desert algae in arid regions, soaking in sterile water, culturing, picking out cultured algae seeds by using a dissecting needle, sterilizing on a sterile glass slide, inoculating into an agar culture dish, and performing sterile culture for 5-10 days to obtain separated and purified algae seeds;
(312) inoculating the separated and purified algae obtained in the step (311) into a culture bottle containing the culture solution obtained in the step (1), and then culturing for 10-20 days at the temperature of 22-35 ℃, the light intensity of 3500Lx and the ventilation volume of 3L/min to obtain algae seeds;
(32) preparation of algae mud
(321) Inoculating the algae seeds obtained in the step (31) into a culture bottle filled with the nutrient solution obtained in the step (1), inoculating algae seeds in a proportion of 0.2-0.5 g per liter of nutrient solution, and performing aerobic culture on algae groups for 10-20 days at the temperature of 22-35 ℃, the light intensity of 3500Lx and the ventilation volume of 3L/min to obtain desert algae;
(322) pouring the desert algae obtained in the step (321) into a filter sieve, filtering to obtain algae slurry, and centrifuging the algae slurry through a centrifuge to remove water to prepare algae mud;
(4) preparation of algal blanket
(41) The plant fiber fabric is spread flatly to form a bottom layer binding belt,
(42) flatly paving the plant fiber net on the bottom layer bonding belt obtained in the step (41) to form a shaping support net, wherein the width of two sides of the bottom layer bonding belt is 2cm with that of two sides of the shaping support net;
(43) spreading the substrate obtained in the step (2) on a shaping support net to form a substrate layer;
(44) uniformly spraying or coating the algae mud obtained in the step (3) on the matrix layer to form an algae seed layer;
(45) covering the upper side of the algae seed layer with a plant fiber fabric to form an upper protective fabric layer;
(46) sewing or adhering the upper protective fiber fabric layer, the shaping support net with the algae seeds and the matrix and the bottom adhesive tape together to form a whole, and drying in the shade to obtain the algae blanket.
Has the advantages that: the algae blanket and the preparation method thereof disclosed by the invention have the following beneficial effects:
1. the fiber net is used as a shaping support net, and various materials such as desert algae, modified plant cellulose water retention materials and the like are processed at one time through a production line. The algae blanket is paved on the ground to form microorganism crusts, cover the ground surface, prevent and control quicksand, keep water and soil, store the water on the ground surface, realize the solidification of the flowing ground surface, treat the bare ground in a severe environment and reduce the erosion of water and wind;
2. the algae blanket can be applied to treatment of flowing sand, hillsides, cliffs, engineering bare land and the like in arid regions, has simple structure, convenient operation and quick arrangement, can be laid in large area in a short time to form a connected fixed ground surface, and plays good roles of preventing wind and fixing sand, keeping water and soil and reducing disasters.
Drawings
FIG. 1 is a schematic view of an algal blanket according to the present disclosure;
FIG. 2 is a flow chart of a method for making an algal blanket according to the present disclosure;
wherein:
| 1-upper protective fiber fabric layer | 2-algal seed layer |
| 3-matrix layer | 4-shaped supporting net |
| 5-bottom adhesive tape |
The specific implementation mode is as follows:
the following describes in detail specific embodiments of the present invention.
Detailed description of the preferred embodiment 1
As shown in figure 1, the algae blanket comprises an upper layer protective fiber fabric layer 1, an algae seed layer 2, a matrix layer 3, a shaping support net 4 and a bottom layer adhesive tape 5 from top to bottom in sequence,
the upper layer protective fiber fabric layer 1, the shaping supporting net 4 and the bottom layer bonding belt 5 are sewn by adopting sewing threads or bonded by adopting modified starch glue,
the matrix layer 3 is bonded with the shaping support net 4,
the algae seed layer 2 is bonded with the matrix layer 3,
the bottom layer adhesive tape 5 is a fiber fabric, and both sides of the fiber fabric are respectively wider than both sides of the shaping support net 4 by 2cm and used as adhesive tapes for adhering the algae blankets to each other.
Further, the thickness of the upper protective fiber fabric layer 1 was 0.2 mm. The upper layer protective fiber fabric layer 1 is a degradable plant fiber fabric, and is a single-layer or multi-layer plant fiber fabric. The required service life of the protective fiber fabric is longer than 30 days, and the algae and the matrix thereof are protected, thereby being beneficial to the growth of the algae.
Further, the thickness of the algal species layer 2 was 0.2 mm. The algae species are a combination of various algae adapted to the climatic conditions of the engineering area. In arid desert areas of China, the algae are mainly Microcoleus vaginatus, Pseudocladosporium javanicus, Bifidobacterium monoides, Nostoc, Schistosoma and Protococcus. The colonization algae species of desert algae are Microcoleus vaginatus and Pseudocladium javanicum, and their proportion is 60-90% of algae species composition.
Further, the thickness of the substrate layer 3 is 2.4 mm. The modified cellulose polymer water-absorbing material has the functions of absorbing and retaining water, provides water, nutrition and an incubation bed for algae, and has the requirements of slow decay of water-retaining capacity, easy degradation, no hardening and no harm to the environment.
Further, the thickness of the shaping support net 4 is 2.0 mm. The shaped support net 4 is a plant fiber net.
Further, the thickness of the underlying adhesive tape 5 is 0.2 mm. The bottom layer bonding belt 5 is a degradable plant fiber fabric and is a single-layer plant fiber fabric. The required service life of the adhesive tape is longer than 30 days, and the algae seeds and the matrix thereof are protected, so that the growth of the algae seeds is facilitated.
As shown in fig. 2, a method for preparing the algal blanket comprises the following steps:
(1) preparation of nutrient solution
(11) Preparing nutrients:
weighing 0.8 part by mass of sodium nitrate, 0.02 part by mass of dipotassium hydrogen phosphate, 0.06 part by mass of magnesium sulfate, 0.02 part by mass of calcium chloride, 0.004 part by mass of citric acid, 0.004 part by mass of ferric ammonium citrate, 0.001 part by mass of disodium ethylenediamine tetraacetic acid and 0.01 part by mass of sodium carbonate, and uniformly mixing the raw materials to obtain mixed powder for later use;
(12) preparing a solution of trace elements
2.86 parts by mass of H3BO31.81 parts by mass of MnCl2·H2O, 0.222 parts by mass of ZnSO4·7H2O, 0.079 parts by mass of CuSO4·5H2O, 0.390 part by mass of Na2MoO4·2H2O, 0.049 part by mass of Co (NO)3)2·6H2Adding O into a proper amount of water to prepare 1000 parts by mass of trace element solution,
(13) mixing the mixed powder obtained in the step (11) with the trace element solution obtained in the step (12) according to a mass ratio of 1.4: 1, preparing and uniformly mixing to prepare nutrient solution;
(2) preparation of the substrate
(21) Uniformly mixing 2.0 parts by mass of modified cellulose, 0.1 part by mass of modified starch, 20.0 parts by mass of cotton wool fibers, 1.0 part by mass of the nutrient solution obtained in the step (1), 10.0 parts by mass of attapulgite powder and 66.9 parts by mass of water to obtain a mixture;
(22) uniformly stirring the mixture obtained in the step (21) to prepare a colloid material, namely obtaining a matrix;
(3) preparation of desert algae
(31) Algal species acquisition
(311) Grinding the soil crust with desert algae in arid regions, soaking in sterile water, culturing, picking out the cultured algae seeds by using a dissecting needle, sterilizing on a sterile glass slide, inoculating into an agar culture dish, and performing sterile culture for 5 days to obtain separated and purified algae seeds;
(312) inoculating the separated and purified algae obtained in the step (311) into a culture bottle containing the culture solution obtained in the step (1), and then culturing for 10 days at the temperature of 22 ℃, the light intensity of 3500Lx and the ventilation volume of 3L/min to obtain algae seeds;
(32) preparation of algae mud
(321) Inoculating the algae seeds obtained in the step (31) into a culture bottle filled with the nutrient solution obtained in the step (1), inoculating algae seeds with the inoculation ratio of 0.2 g per liter of nutrient solution, and culturing algae groups for 10 days in an aerated manner at the temperature of 22 ℃, the light intensity of 3500Lx and the aeration quantity of 3L/min to obtain desert algae;
(322) pouring the desert algae obtained in the step (321) into a filter sieve, filtering to obtain algae slurry, and centrifuging the algae slurry through a centrifuge to remove water to prepare algae mud;
(4) preparation of algal blanket
(41) The plant fiber fabric is spread flatly to form a bottom layer binding belt,
(42) flatly paving the plant fiber net on the bottom layer bonding belt obtained in the step (41) to form a shaping support net, wherein the width of two sides of the bottom layer bonding belt is 2cm with that of two sides of the shaping support net;
(43) spreading the substrate obtained in the step (2) on a shaping support net to form a substrate layer;
(44) uniformly spraying or coating the algae mud obtained in the step (3) on the matrix layer to form an algae seed layer;
(45) covering the upper side of the algae seed layer with a plant fiber fabric to form an upper protective fabric layer;
(46) sewing the upper protective fiber fabric layer, the shaping support net with the algae seeds and the matrix and the bottom adhesive tape together to form a whole, and drying in the shade to obtain the algae blanket.
Specific example 2
An algae blanket comprises an upper layer protective fiber fabric layer 1, an algae seed layer 2, a matrix layer 3, a shaping support net 4 and a bottom layer bonding belt 5 from top to bottom in sequence,
the upper layer protective fiber fabric layer 1, the shaping supporting net 4 and the bottom layer bonding belt 5 are sewn by adopting sewing threads or bonded by adopting modified starch glue,
the matrix layer 3 is bonded with the shaping support net 4,
the algae seed layer 2 is bonded with the matrix layer 3,
the bottom layer adhesive tape 5 is a fiber fabric, and both sides of the fiber fabric are respectively wider than both sides of the shaping support net 4 by 2cm and used as adhesive tapes for adhering the algae blankets to each other.
Further, the thickness of the upper protective fiber fabric layer 1 was 0.3 mm. The upper layer protective fiber fabric layer 1 is a degradable plant fiber fabric, and is a single-layer or multi-layer plant fiber fabric. The required service life of the protective fiber fabric is longer than 30 days, and the algae and the matrix thereof are protected, thereby being beneficial to the growth of the algae.
Further, the thickness of the algal species layer 2 was 0.3 mm. The algae species are a combination of various algae adapted to the climatic conditions of the engineering area. In arid desert areas of China, the algae are mainly Microcoleus vaginatus, Pseudocladosporium javanicus, Bifidobacterium monoides, Nostoc, Schistosoma and Protococcus. The colonization algae species of the desert algae are Microcoleus vaginatus and Pseudocladium javanicum, and the proportion of the colonization algae species accounts for 60-90% of the algae species composition.
Further, the thickness of the substrate layer 3 is 2.6 mm. The modified cellulose polymer water-absorbing material has the functions of absorbing and retaining water, provides water, nutrition and an incubation bed for algae, and has the requirements of slow decay of water-retaining capacity, easy degradation, no hardening and no harm to the environment.
Further, the thickness of the shaping support net 4 is 2.5 mm. The shaped support net 4 is a plant fiber net.
Further, the thickness of the underlying adhesive tape 5 is 0.3 mm. The bottom layer bonding belt 5 is a degradable plant fiber fabric and is a single-layer plant fiber fabric. The required service life of the adhesive tape is longer than 30 days, and the algae seeds and the matrix thereof are protected, so that the growth of the algae seeds is facilitated.
A preparation method of the algae blanket comprises the following steps:
(1) preparation of nutrient solution
(11) Preparing nutrients:
weighing 2.5 parts by mass of sodium nitrate, 0.05 part by mass of dipotassium hydrogen phosphate, 0.09 part by mass of magnesium sulfate, 0.05 part by mass of calcium chloride, 0.008 part by mass of citric acid, 0.008 part by mass of ferric ammonium citrate, 0.003 part by mass of disodium ethylenediamine tetraacetic acid and 0.04 part by mass of sodium carbonate, and uniformly mixing the raw materials to obtain mixed powder for later use;
(12) preparing a solution of trace elements
2.86 parts by mass of H3BO31.81 parts by mass of MnCl2·H2O, 0.222 parts by mass of ZnSO4·7H2O, 0.079 parts by mass of CuSO4·5H2O, 0.390 part by mass of Na2MoO4·2H2O, 0.049 part by mass of Co (NO)3)2·6H2Adding O into a proper amount of water to prepare 1000 parts by mass of trace element solution,
(13) mixing the mixed powder obtained in the step (11) with the trace element solution obtained in the step (12) according to a mass ratio of 1.8: 1, preparing and uniformly mixing to prepare nutrient solution;
(2) preparation of the substrate
(21) Uniformly mixing 2.0 parts by mass of modified cellulose, 0.1 part by mass of modified starch, 20.0 parts by mass of cotton wool fibers, 1.0 part by mass of the nutrient solution obtained in the step (1), 10.0 parts by mass of attapulgite powder and 66.9 parts by mass of water to obtain a mixture;
(22) uniformly stirring the mixture obtained in the step (21) to prepare a colloid material, namely obtaining a matrix;
(3) preparation of desert algae
(31) Algal species acquisition
(311) Grinding the soil crust with desert algae in arid region, soaking in sterile water, culturing, picking out cultured algae seeds with dissecting needle, sterilizing on sterile slide glass, inoculating into agar culture dish, and performing sterile culture for 10 days to obtain separated and purified algae seeds;
(312) inoculating the separated and purified algae obtained in the step (311) into a culture bottle containing the culture solution obtained in the step (1), and then culturing for 20 days at the temperature of 35 ℃, the light intensity of 3500Lx and the ventilation volume of 3L/min to obtain algae seeds;
(32) preparation of algae mud
(321) Inoculating the algae seeds obtained in the step (31) into a culture bottle filled with the nutrient solution obtained in the step (1), inoculating algae seeds with the inoculation proportion of 0.5 g per liter of nutrient solution, and culturing algae groups for 20 days in an aeration mode under the conditions that the temperature is 35 ℃, the light intensity is 3500Lx and the aeration quantity is 3L/min to obtain desert algae;
(322) pouring the desert algae obtained in the step (321) into a filter sieve, filtering to obtain algae slurry, and centrifuging the algae slurry through a centrifuge to remove water to prepare algae mud;
(4) preparation of algal blanket
(41) The plant fiber fabric is spread flatly to form a bottom layer binding belt,
(42) flatly paving the plant fiber net on the bottom layer bonding belt obtained in the step (41) to form a shaping support net, wherein the width of two sides of the bottom layer bonding belt is 2cm with that of two sides of the shaping support net;
(43) spreading the substrate obtained in the step (2) on a shaping support net to form a substrate layer;
(44) uniformly spraying or coating the algae mud obtained in the step (3) on the matrix layer to form an algae seed layer;
(45) covering the upper side of the algae seed layer with a plant fiber fabric to form an upper protective fabric layer;
(46) and (3) bonding the upper protective fiber fabric layer, the shaping support net with the algae seeds and the matrix and the bottom adhesive tape together to form a whole, and drying in the shade to obtain the algae blanket.
Specific example 3
An algae blanket comprises an upper layer protective fiber fabric layer 1, an algae seed layer 2, a matrix layer 3, a shaping support net 4 and a bottom layer bonding belt 5 from top to bottom in sequence,
the upper layer protective fiber fabric layer 1, the shaping supporting net 4 and the bottom layer bonding belt 5 are sewn by adopting sewing threads or bonded by adopting modified starch glue,
the matrix layer 3 is bonded with the shaping support net 4,
the algae seed layer 2 is bonded with the matrix layer 3,
the bottom layer adhesive tape 5 is a fiber fabric, and both sides of the fiber fabric are respectively wider than both sides of the shaping support net 4 by 2cm and used as adhesive tapes for adhering the algae blankets to each other.
Further, the thickness of the upper protective fiber fabric layer 1 was 0.25 mm. The upper layer protective fiber fabric layer 1 is a degradable plant fiber fabric, and is a single-layer or multi-layer plant fiber fabric. The required service life of the protective fiber fabric is longer than 30 days, and the algae and the matrix thereof are protected, thereby being beneficial to the growth of the algae.
Further, the thickness of the algal species layer 2 was 0.25 mm. The algae species are a combination of various algae adapted to the climatic conditions of the engineering area. In arid desert areas of China, the algae are mainly Microcoleus vaginatus, Pseudocladosporium javanicus, Bifidobacterium monoides, Nostoc, Schistosoma and Protococcus. The colonization algae species of desert algae are Microcoleus vaginatus and Pseudocladium javanicum, and their proportion is 60-90% of algae species composition.
Further, the thickness of the substrate layer 3 is 2.5 mm. The modified cellulose polymer water-absorbing material has the functions of absorbing and retaining water, provides water, nutrition and an incubation bed for algae, and has the requirements of slow decay of water-retaining capacity, easy degradation, no hardening and no harm to the environment.
Further, the thickness of the shaping support net is 2.3 mm. The shaping support net is a plant fiber net.
Further, the thickness of the underlying adhesive tape 5 is 0.25 mm. The bottom layer bonding belt 5 is a degradable plant fiber fabric and is a single-layer plant fiber fabric. The required service life of the adhesive tape is longer than 30 days, and the algae seeds and the matrix thereof are protected, so that the growth of the algae seeds is facilitated.
A preparation method of the algae blanket comprises the following steps:
(1) preparation of nutrient solution
(11) Preparing nutrients:
weighing 1 part by mass of sodium nitrate, 0.04 part by mass of dipotassium hydrogen phosphate, 0.07 part by mass of magnesium sulfate, 0.04 part by mass of calcium chloride, 0.006 part by mass of citric acid, 0.006 part by mass of ferric ammonium citrate, 0.002 part by mass of disodium ethylene diamine tetraacetate and 0.03 part by mass of sodium carbonate, and uniformly mixing the raw materials to obtain mixed powder for later use;
(12) preparing a solution of trace elements
2.86 parts by mass of H3BO31.81 parts by mass of MnCl2·H2O, 0.222 parts by mass of ZnSO4·7H2O, 0.079 parts by mass of CuSO4·5H2O, 0.390 part by mass of Na2MoO4·2H2O, 0.049 part by mass of Co (NO)3)2·6H2Adding O into a proper amount of water to prepare 1000 parts by mass of trace element solution,
(13) mixing the mixed powder obtained in the step (11) with the trace element solution obtained in the step (12) according to a mass ratio of 1.6: 1, preparing and uniformly mixing to prepare nutrient solution;
(2) preparation of the substrate
(21) Uniformly mixing 2.0 parts by mass of modified cellulose, 0.1 part by mass of modified starch, 20.0 parts by mass of cotton wool fibers, 1.0 part by mass of the nutrient solution obtained in the step (1), 10.0 parts by mass of attapulgite powder and 66.9 parts by mass of water to obtain a mixture;
(22) uniformly stirring the mixture obtained in the step (21) to prepare a colloid material, namely obtaining a matrix;
(3) preparation of desert algae
(31) Algal species acquisition
(311) Grinding the soil crust with desert algae in arid regions, soaking in sterile water, culturing, picking out the cultured algae seeds by using a dissecting needle, sterilizing on a sterile glass slide, inoculating into an agar culture dish, and performing sterile culture for 8 days to obtain separated and purified algae seeds;
(312) inoculating the separated and purified algae obtained in the step (311) into a culture bottle containing the culture solution obtained in the step (1), and then culturing for 15 days at the temperature of 30 ℃, the light intensity of 3500Lx and the ventilation volume of 3L/min to obtain algae seeds;
(32) preparation of algae mud
(321) Inoculating the algae seeds obtained in the step (31) into a culture bottle filled with the nutrient solution obtained in the step (1), inoculating algae seeds with the inoculation ratio of 0.3 g per liter of nutrient solution, and culturing algae groups for 15 days in an aeration manner at the temperature of 30 ℃, the light intensity of 3500Lx and the aeration quantity of 3L/min to obtain desert algae;
(322) pouring the desert algae obtained in the step (321) into a filter sieve, filtering to obtain algae slurry, and centrifuging the algae slurry through a centrifuge to remove water to prepare algae mud;
(4) preparation of algal blanket
(41) The plant fiber fabric is spread flatly to form a bottom layer binding belt,
(42) flatly paving the plant fiber net on the bottom layer bonding belt obtained in the step (41) to form a shaping support net, wherein the width of two sides of the bottom layer bonding belt is 2cm with that of two sides of the shaping support net;
(43) spreading the substrate obtained in the step (2) on a shaping support net to form a substrate layer;
(44) uniformly spraying or coating the algae mud obtained in the step (3) on the matrix layer to form an algae seed layer;
(45) covering the upper side of the algae seed layer with a plant fiber fabric to form an upper protective fabric layer;
(46) and (3) bonding the upper protective fiber fabric layer, the shaping support net with the algae seeds and the matrix and the bottom adhesive tape together to form a whole, and drying in the shade to obtain the algae blanket.
The embodiments of the present invention have been described in detail. However, the present invention is not limited to the above-described embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.
Claims (1)
1. An algae blanket is characterized by sequentially comprising an upper layer protective fiber fabric layer, an algae seed layer, a matrix layer, a shaping support net and a bottom layer adhesive tape from top to bottom,
the upper layer protective fiber fabric layer, the shaping support net and the bottom layer bonding belt are sewn by adopting sewing threads or bonded by adopting modified starch glue,
the substrate layer is bonded with the shaping support net,
the algae seed layer is adhered to the matrix layer,
the bottom layer bonding tape is a fiber fabric, and two sides of the bottom layer bonding tape are respectively wider than two sides of the shaping support net by 2cm and are used as bonding tapes for bonding the algal blankets; the thickness of the upper protective fiber fabric layer is 0.2-0.3 mm; the thickness of the algae seed layer is 0.2-0.3 mm; the thickness of the substrate layer is 2.4-2.6 mm; the thickness of the shaping support net is 2.0-2.5 mm; the thickness of the bottom layer adhesive tape is 0.2-0.3 mm;
the preparation method of the algal blanket comprises the following steps:
(1) preparation of nutrient solution
(11) Preparing nutrients:
weighing 0.8-2.5 parts by mass of sodium nitrate, 0.02-0.05 part by mass of dipotassium hydrogen phosphate, 0.06-0.09 part by mass of magnesium sulfate, 0.02-0.05 part by mass of calcium chloride, 0.004-0.008 part by mass of citric acid, 0.004-0.008 part by mass of ferric ammonium citrate, 0.001-0.003 part by mass of disodium ethylenediamine tetraacetic acid and 0.01-0.04 part by mass of sodium carbonate, and uniformly mixing the raw materials to obtain mixed powder for later use;
(12) preparing a solution of trace elements
2.86 parts by mass of H3BO31.81 parts by mass of MnCl2·H2O, 0.222 parts by mass of ZnSO4·7H2O, 0.079 parts by mass of CuSO4·5H2O, 0.390 part by mass of Na2MoO4·2H2O, 0.049 part by mass of Co (NO)3)2·6H2Adding O into a proper amount of water to prepare 1000 parts by mass of trace element solution,
(13) mixing the mixed powder obtained in the step (11) with the trace element solution obtained in the step (12) according to the mass ratio of (1.4-1.8): 1, preparing and uniformly mixing to prepare nutrient solution;
(2) preparation of the substrate
(21) Uniformly mixing 2.0 parts by mass of modified cellulose, 0.1 part by mass of modified starch, 20.0 parts by mass of cotton wool fibers, 1.0 part by mass of the nutrient solution obtained in the step (1), 10.0 parts by mass of attapulgite powder and 66.9 parts by mass of water to obtain a mixture;
(22) uniformly stirring the mixture obtained in the step (21) to prepare a colloid material, namely obtaining a matrix;
(3) preparation of desert algae
(31) Algal species acquisition
(311) Grinding the soil crust with desert algae in arid region, soaking in sterile water, culturing, picking out cultured algae seeds with dissecting needle, sterilizing on sterile glass slide,
inoculating into an agar culture dish, and performing aseptic culture for 5-10 days to obtain separated and purified algae seeds;
(312) inoculating the separated and purified algae obtained in the step (311) into a culture bottle containing the culture solution obtained in the step (1), then, at the temperature of 22-35 ℃,
culturing for 10-20 days under the conditions that the light intensity is 3500Lx and the ventilation volume is 3L/min to obtain algae seeds;
(32) preparation of algae mud
(321) Inoculating the algae seeds obtained in the step (31) into a culture bottle filled with the nutrient solution obtained in the step (1), inoculating algae seeds in a proportion of 0.2-0.5 g per liter of nutrient solution, and performing aerobic culture on algae groups for 10-20 days at the temperature of 22-35 ℃, the light intensity of 3500Lx and the ventilation volume of 3L/min to obtain desert algae;
(322) pouring the desert algae obtained in the step (321) into a filter sieve, filtering to obtain algae slurry, and centrifuging the algae slurry through a centrifuge to remove water to prepare algae mud;
(4) preparation of algal blanket
(41) The plant fiber fabric is spread flatly to form a bottom layer binding belt,
(42) flatly paving the plant fiber net on the bottom layer bonding belt obtained in the step (41) to form a shaping support net, wherein the width of two sides of the bottom layer bonding belt is 2cm with that of two sides of the shaping support net;
(43) spreading the substrate obtained in the step (2) on a shaping support net to form a substrate layer;
(44) uniformly spraying or coating the algae mud obtained in the step (3) on the matrix layer to form an algae seed layer;
(45) covering the upper side of the algae seed layer with a plant fiber fabric to form an upper protective fabric layer;
(46) sewing or adhering the upper protective fiber fabric layer, the shaping support net with the algae seeds and the matrix and the bottom adhesive tape together to form a whole, and drying in the shade to obtain the algae blanket.
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