CN113943581A - Application of biological skinning composition to rock slope - Google Patents
Application of biological skinning composition to rock slope Download PDFInfo
- Publication number
- CN113943581A CN113943581A CN202111038548.1A CN202111038548A CN113943581A CN 113943581 A CN113943581 A CN 113943581A CN 202111038548 A CN202111038548 A CN 202111038548A CN 113943581 A CN113943581 A CN 113943581A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- composition
- biological
- bacillus
- skinning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 92
- 239000011435 rock Substances 0.000 title claims abstract description 59
- 239000002245 particle Substances 0.000 claims abstract description 27
- 239000010902 straw Substances 0.000 claims description 41
- 239000001963 growth medium Substances 0.000 claims description 39
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 34
- 241000194108 Bacillus licheniformis Species 0.000 claims description 31
- 239000012634 fragment Substances 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 21
- 239000004202 carbamide Substances 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000003795 chemical substances by application Substances 0.000 claims description 18
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 15
- 239000002689 soil Substances 0.000 claims description 15
- 230000000813 microbial effect Effects 0.000 claims description 13
- 238000005507 spraying Methods 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 11
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 10
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 10
- 235000015278 beef Nutrition 0.000 claims description 10
- 229940041514 candida albicans extract Drugs 0.000 claims description 10
- 238000007865 diluting Methods 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 10
- 239000012137 tryptone Substances 0.000 claims description 10
- 239000012138 yeast extract Substances 0.000 claims description 10
- 241000193469 Clostridium pasteurianum Species 0.000 claims description 8
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 29
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 abstract description 18
- 230000003628 erosive effect Effects 0.000 abstract description 12
- 244000005700 microbiome Species 0.000 abstract description 11
- 108010046334 Urease Proteins 0.000 abstract description 10
- 229910000019 calcium carbonate Inorganic materials 0.000 abstract description 9
- 230000004083 survival effect Effects 0.000 abstract description 5
- 239000013078 crystal Substances 0.000 abstract description 4
- 239000004575 stone Substances 0.000 description 30
- 206010039509 Scab Diseases 0.000 description 28
- 238000011081 inoculation Methods 0.000 description 23
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 241000195493 Cryptophyta Species 0.000 description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 4
- 229910001424 calcium ion Inorganic materials 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 235000003826 Artemisia Nutrition 0.000 description 3
- 235000003261 Artemisia vulgaris Nutrition 0.000 description 3
- 241000192700 Cyanobacteria Species 0.000 description 3
- 241000186547 Sporosarcina Species 0.000 description 3
- 241000193395 Sporosarcina pasteurii Species 0.000 description 3
- 244000030166 artemisia Species 0.000 description 3
- 235000009052 artemisia Nutrition 0.000 description 3
- 230000033558 biomineral tissue development Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000004021 humic acid Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229910021532 Calcite Inorganic materials 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 description 1
- 241000179039 Paenibacillus Species 0.000 description 1
- 241000606860 Pasteurella Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- -1 feed processing Substances 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 125000003147 glycosyl group Chemical group 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000006911 nucleation Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2105/00—Erosion prevention
Abstract
The invention provides an application of a biological crust composition on a rock slope, which relates to the technical field of microorganism application, and is characterized in that calcium carbonate crystals are generated between biological crust particles and exposed rocks by a solidified composition containing urease-producing microorganisms, and then the crust composition and the exposed rock surface are combined together, so that the negative effects of wind erosion and rain erosion on lichen survival are reduced, and the effect of improving the lichen survival rate is achieved.
Description
Technical Field
The invention relates to the technical field of microorganism application, in particular to application of a biological crust composition to a rock slope.
Background
Biological soil crusts (biological soil crusts) are also called biological soil crusts, soil microorganism crusts and the like, are compounds formed by bonding cryptophytes such as micro-bacteria, fungi, algae, lichens, moss and the like and hyphae, secretions and the like thereof with soil gravels, and are important ground surface covering types in arid and semi-arid regions. The existence of the biological crust has important significance on the aspects of desert fixation, the physical, chemical and biological characteristics of the soil surface, the wind erosion and water erosion resistance of the soil and the like. At present, methods for preventing wind erosion desertification, rock desertification and water erosion desertification by using biological crust are reported, and after the biological crust is formed, infiltration can be increased during rainfall, surface runoff is reduced, so that the soil is prevented from being eroded by running water, and water and soil are kept.
Lichen in the biological crust is a pioneer organism of the earth, and the unique biological characteristics of the lichen are that mechanical protection is provided by wrapping algae or cyanobacteria inside fungal mycelia, and the harm of the natural over-high light intensity to the algae and the cyanobacteria is reduced; the algae and cyanobacteria wrapped in the fungal mycelium can fix carbon dioxide in the air, and can synthesize carbohydrates necessary for the survival of the fungi by utilizing trace water under natural conditions. The existence mode of reciprocal symbiosis enables lichen to be better than other organisms and more suitable for developing and living in various extreme habitats, such as: polar region, desert, mountain; lichen growth substrates are more diverse, often on rock surfaces, in rock interiors, ground, bark and trunks, and can even grow on the surface of metal and animal bone debris. In the technical field of slope ecological restoration, lichens are topologically colonized on a bare slope, and are symbiotically mutually through a self-nutrition mode, so that the ecological environment condition is improved and nutrition is accumulated while self life support is maintained, thereby being beneficial to subsequent colonization of other microorganisms and plants and building an ecological system conforming to the succession rule of natural organisms; although the lichen grows slowly, once colonized, can survive for a long time, is not influenced by seasons, is evergreen in all seasons, slowly releases synthesized nutrient substances to the environment, only needs a small amount of external earthing in the whole process, does not need repeated inoculation, needs less manpower, is simple and easy in engineering, and only needs short-term maintenance after construction.
In the prior art, biological crust is widely applied to scenes such as ecological restoration of degraded alpine grassland or mine vegetation restoration, and the like, and the technical effects of good vegetation restoration and no ecological risk are achieved; however, when the biological crust is applied to a bare rock slope, the surface of the rock is low in crust coverage rate due to the fact that organic matters on the surface of the rock are few and the rock is seriously washed by wind and rain.
Thus, there is a need for a biological skinning composition for rock slopes that is adapted to bare rock slopes.
Disclosure of Invention
In view of the problems, the invention provides an application of a biological skinning composition to a rock slope surface, and the skinning coverage rate of the rock slope surface is better.
In order to achieve the purpose, the application of the biological skinning composition to the rock slope is used for laying the skinning composition prepared by mixing biological skinning particles, straw particles and a water-retaining agent according to a proportion on the rock slope;
and spraying a curing composition prepared by mixing a curing microbial agent, urea and calcium chloride according to a proportion onto the laid skinning composition.
Further, preferably, the biological crust particles are prepared by taking down lichen and 4-10mm of soil below the lichen at the same time, crushing, and filtering with a screen with a diameter of 3-5 mm to obtain lichen fragments with uniform size.
Further, preferably, the manufacturing method of the straw particles comprises the following steps:
crushing the straws into straw fragments of 3-5 cm;
soaking the straw fragments for 24-72 hours, and then drying to control the water content to be 20%;
making the dried straw fragments into straw particles with the fineness of 0.1 mm.
Further, preferably, the method for producing the solidified microbial preparation includes:
inoculating Papanicolaou bacillus to Papanicolaou bacillus culture medium solution, wherein the Papanicolaou bacillus culture medium solution comprises 10g/L tryptone, 5g/L beef extract, 6.5g/L yeast extract, 5g/L, NaCl1g/L ammonium sulfate and H2A culture medium of O1L; adding urea into the culture medium solution until the concentration is 10g/L, and addingNiCl2To a concentration of 4 g/L; culturing and diluting the bacillus pasteurianus liquid at the rotation speed of 130rmp/min and the OD600 of 0.7-0.9 under the conditions of 31 ℃ and pH of 8.1;
inoculating Bacillus licheniformis to a Bacillus licheniformis culture medium solution, wherein the Bacillus licheniformis culture medium solution comprises tryptone 10g/L, beef extract 5g/L, yeast extract 6.5g/L, ammonium sulfate 5g/L, NaCl1g/L and H2A culture medium of O1L; adding urea to the culture medium solution to reach the concentration of 10g/L, and adding NiCl2To a concentration of 4 g/L; culturing and diluting the bacillus licheniformis liquid at 31 ℃ and pH 8.1 with the rotating speed of a shaking table of 130rmp/min until OD600 is 0.7-0.9;
and preparing the bacillus pasteurianus bacterial liquid and the bacillus licheniformis bacterial liquid into a solidified microbial agent according to the volume ratio of 3-5: 1.
The biological crust composition for the rock slope surface utilizes a calcium carbonate biomineralization technology of solidified microorganisms, when urease-producing microorganisms are in a urea-calcium ion environment, urease can be generated through metabolism, and the urease decomposes urea into ammonia and carbon dioxide, so that the concentration of local carbonate ions is increased; after a cementing liquid such as calcium chloride is added into the surrounding environment, carbonate ions and calcium ions are combined to generate precipitates, calcium carbonate crystals can be rapidly separated out among biological skinning particles and between biological skinning and exposed rock surfaces, and the skinning composition and the exposed rock surfaces are combined together; the influence of wind erosion and rain erosion on the skinning coverage rate in the bare rock scene is weakened, so that the biological skinning coverage rate of the bare rock slope is improved.
Drawings
FIG. 1 shows a graph of lichen growth 18 days after inoculation in the experimental group of the biological skinning composition for rock slopes according to the invention;
FIG. 2 shows a perspective view of experimental points in an example of the effect of the bio-skinning composition according to the invention for rock slopes;
FIG. 3 shows a close-up view of experimental points in an example of the effect of the bio-skinning composition according to the invention for rock slopes;
FIG. 4 is a graph showing the effect of group B3 on the day of inoculation in an example of the effect of the biological skinning composition for rock slopes according to the present invention;
FIG. 5 is a graph showing the lichen growth effect of group B3 inoculated for 22 days in an example of the effect of the biological skinning composition for rock slopes according to the present invention;
fig. 6 is a graph showing the effect of lichen growth after 4 months of inoculation of group B3 in an example of the effect of the biofouling composition of the present invention for rock slopes;
fig. 7 is a graph showing the effect of lichen growth around 4 months after inoculation of group B3 in an example of the effect of the biofouling composition of the present invention on rock slopes.
Detailed Description
It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. The examples do not show specific techniques or conditions, and the reagents or apparatuses used are not shown in the specifications of the products, and the conventional products are available from normal distributors.
MICP (microbial induced carbonate precipitation-induced calcium carbonate) is a biological induced mineralization which widely exists in nature, and microorganisms are metabolized to produce carbon dioxide and calcium carbonate ions, which are environments on which calcium carbonate precipitation depends. Among them, the calcium carbonate biomineralization technique that is widely used is MICP by urea hydrolysis. When the urease-producing microorganisms are in a urea-calcium ion environment, urease can be produced through metabolism, the urease decomposes urea into ammonia and carbon dioxide, the concentration of local carbonate ions is increased, and meanwhile, cell walls of thallus with negative charges can chelate calcium ions, so that calcite is mineralized and deposited, and is not only insoluble in water, but also has good compatibility with stone-based materials.
The strains used in the present invention are Bacillus licheniformis (Bacil-luxicheniformis) and Bacillus pasteurianus (Sporosarcinapasturii); among them, Bacillus licheniformis has a wide habitat and is found in both terrestrial and aquatic environments. In recent years, the application reports of bacillus licheniformis in various aspects are increasing at home and abroad, and good research results are obtained in the industries of medicine, feed processing, pesticide and the like. The strain also has strong protease, lipase and amylase activities, particularly the cell surface of bacillus licheniformis and the extracellular polysaccharide EPS can provide nucleation sites for mineral precipitation, the extracellular polymer EPS contains a large amount of tyrosine and glycine, and the two amino acids can attract and combine a large amount of cations under alkaline conditions to provide a local saturated microenvironment for mineral formation; the method is more suitable for scenes with poor adhesion force on rock slopes and the like.
Paecilomyces pasteurianus (Sporosarcina) belongs to the genus Sporosarcina (also known as Sporosarcina pasteurianus) of the family Mycosphaerellales, and is a non-pathogenic gram-positive bacterium isolated from soil. The Paenibacillus pasteurianus is rod-shaped in cell shape, about 2-3 microns in length, high in viability, capable of growing at 15-37 ℃, capable of continuously surviving in the form of spores in the absence of nutrients, round in spore shape and about 0.5-1.5 microns in diameter, and capable of generating urease (urease) serving as urea hydrolase in the presence of urea.
Among them, Bacillus licheniformis (Bacil-Luslicheniformis) and Bacillus pasteurianus (Sporosarcinapasserii) are commercially available.
Example 1
The biological crust particles are prepared by taking down lichen and soil 4-10mm below the lichen at the same time, grinding, and filtering with a screen mesh with the diameter of 3-5 mm to obtain lichen fragments with uniform size.
Wherein, the lichen in the biological crust granules is a local lichen species collected from the mountain of Mentougou Lingshan of Beijing. In a specific implementation, the biological crust particles can also be a mixture of moss crust, lichen crust and algae crust in the local field.
Crushing the straws into straw fragments of 3-5 cm; soaking the straw fragments for 24-72 hours, and then drying to control the water content to be 20%; making the dried straw fragments into straw particles with the fineness of 0.1 mm. Wherein, it needs to be said that the straws are pretreated to remove the sections and sheaths of the straws. In addition, the soaking of the crushed straw fragments needs to be carried out for 24-72 hours, and then the straw fragments enter a pulverization link; the crushed straws are soaked in clear water, so that cellulose and water molecules in the straw fibers can be fully infiltrated, and natural fiber dissociation treatment can be performed on the straws at room temperature and in a humid environment by means of air and microorganisms carried by the straws, so that a small amount of hemicellulose can be removed, a small amount of lignin can be dissolved out, and intercellular layers of straw cells can be softened.
60 parts of biological skinning particles, 20 parts of straw particles and 0.6 part of sodium polyacrylate serving as a water-retaining agent are mixed to prepare the skinning composition I. The water-retaining agent may be sodium polyacrylate, or a mixture of sodium polyacrylate, humic acid (humic acid), minerals, etc., and the growth of lichen can be promoted by adding the water-retaining agent.
In the specific implementation process, the application amount of the first skinning composition is 1.5-3 kg/m2。
Inoculating Papanicolaou bacillus to Papanicolaou bacillus culture medium solution, wherein the Papanicolaou bacillus culture medium solution comprises 10g/L tryptone, 5g/L beef extract, 6.5g/L yeast extract, 5g/L, NaCl1g/L ammonium sulfate and H2A culture medium of O1L; adding urea to the culture medium solution to reach the concentration of 10g/L, and adding NiCl2To a concentration of 4 g/L; culturing at 31 deg.C and pH of 8.1 with shaking table rotation speed of 130rmp/min, and diluting to OD600 of 0.7 to obtain Bacillus pasteurii liquid.
Inoculating Bacillus licheniformis to a Bacillus licheniformis culture medium solution, wherein the Bacillus licheniformis culture medium solution comprises tryptone 10g/L, beef extract 5g/L, yeast extract 6.5g/L, ammonium sulfate 5g/L, NaCl1g/L and H2A culture medium of O1L; adding urea to the culture medium solution to reach the concentration of 10g/L, and adding NiCl2To a concentration of 4 g/L; culturing at 31 deg.C and pH of 8.1 with shaking table rotation speed of 130rmp/min, and diluting to OD600 of 0.7 to obtain Bacillus licheniformis liquid.
Preparing a solidified microbial agent from a bacillus pasteurianus bacterial liquid and a bacillus licheniformis bacterial liquid according to the volume ratio of 3: 1; and forming the solidified composition I by using the solidified microbial agent, 0.5mol/L urea and 0.5mol/L calcium chloride according to the volume ratio of 3:2: 3.
In the specific implementation process, the application amount of the first curing composition is 3-4L/m2。
Example 2
The biological crust particles are prepared by taking down lichen and soil 4-10mm below the lichen at the same time, grinding, and filtering with a screen mesh with the diameter of 3-5 mm to obtain lichen fragments with uniform size.
Crushing the straws into straw fragments of 3-5 cm; soaking the straw fragments for 24-72 hours, and then drying to control the water content to be 20%; making the dried straw fragments into straw particles with the fineness of 0.1 mm.
80 parts of biological skinning particles, 30 parts of straw particles and 0.9 part of artemisia glue serving as a water-retaining agent are mixed to prepare a skinning composition II. Wherein, the artemisia glue mainly comprises glycosyl groups such as glucose, galactose, mannose, rhamnose, arabinose, xylose and the like and uronic acid, and can be biodegraded; on the basis of improving the water absorption, water retention and permeability of the lichen particles and calcium carbonate crystals formed on the surface of the rock, the stability is better, and the wind erosion resistance and the water erosion resistance are further improved.
Inoculating Papanicolaou bacillus to Papanicolaou bacillus culture medium solution, wherein the Papanicolaou bacillus culture medium solution comprises 10g/L tryptone, 5g/L beef extract, 6.5g/L yeast extract, 5g/L, NaCl1g/L ammonium sulfate and H2A culture medium of O1L; adding urea to the culture medium solution to reach the concentration of 10g/L, and adding NiCl2To a concentration of 4 g/L; culturing at 31 deg.C and pH of 8.1 with shaking table rotation speed of 130rmp/min, and diluting to OD600 of 0.9 to obtain Bacillus pasteurii liquid.
Inoculating Bacillus licheniformis to a Bacillus licheniformis culture medium solution, wherein the Bacillus licheniformis culture medium solution comprises tryptone 10g/L, beef extract 5g/L, yeast extract 6.5g/L, ammonium sulfate 5g/L, NaCl1g/L and H2A culture medium of O1L; adding urine to the medium solutionAdding NiCl until the concentration is 10g/L2To a concentration of 4 g/L; culturing at 31 deg.C and pH of 8.1 with shaking table rotation speed of 130rmp/min, and diluting to OD600 of 0.9 to obtain Bacillus licheniformis liquid.
Preparing a solidified microbial agent from a pasteurella bacteria liquid and a bacillus licheniformis liquid according to the volume ratio of 5: 1; and forming the solidified composition II by using the solidified microbial agent, 0.5mol/L urea and 0.5mol/L calcium chloride according to the volume ratio of 3:2: 3.
Example 3
The biological crust particles are prepared by taking down lichen and soil 4-10mm below the lichen at the same time, grinding, and filtering with a screen mesh with the diameter of 3-5 mm to obtain lichen fragments with uniform size.
Crushing the straws into straw fragments of 3-5 cm; soaking the straw fragments for 24-72 hours, and then drying to control the water content to be 20%; making the dried straw fragments into straw particles with the fineness of 0.1 mm.
70 parts of biological crust particles, 20 parts of straw particles and 0.8 part of artemisia glue serving as a water-retaining agent are mixed to prepare a crust composition III.
Inoculating Papanicolaou bacillus to Papanicolaou bacillus culture medium solution, wherein the Papanicolaou bacillus culture medium solution comprises 10g/L tryptone, 5g/L beef extract, 6.5g/L yeast extract, 5g/L, NaCl1g/L ammonium sulfate and H2A culture medium of O1L; adding urea to the culture medium solution to reach the concentration of 10g/L, and adding NiCl2To a concentration of 4 g/L; culturing at 31 deg.C and pH of 8.1 with shaking table rotation speed of 130rmp/min, and diluting to OD600 of 0.8 to obtain Bacillus pasteurii liquid.
Inoculating Bacillus licheniformis to a Bacillus licheniformis culture medium solution, wherein the Bacillus licheniformis culture medium solution comprises tryptone 10g/L, beef extract 5g/L, yeast extract 6.5g/L, ammonium sulfate 5g/L, NaCl1g/L and H2A culture medium of O1L; adding urea to the culture medium solution to reach the concentration of 10g/L, and adding NiCl2To a concentration of 4 g/L; culturing at 31 deg.C and pH of 8.1 with shaking table rotation speed of 130rmp/min, and diluting to OD600 of 0.8 to obtain Bacillus licheniformis liquid.
Preparing a solidified microbial agent from a bacillus pasteurianus bacterial liquid and a bacillus licheniformis bacterial liquid according to the volume ratio of 9: 2; and forming a third solidified composition by using the solidified microbial agent, 0.5mol/L urea and 0.5mol/L calcium chloride according to the volume ratio of 3:2: 3.
Experimental example 1
Selecting sample stones with similar flatness at the Ministry Tou-Gong-Gai mountain of Beijing at 28 days 10 and 28 months in 2020, and transporting the sample stones to a laboratory; dividing the surface of the sample stone into 20 parts of 0.5m each2The experimental area of (a); the 20 experimental areas were divided into five groups A1-A5, each group containing 4 experimental areas. The test results were averaged and tested under the same conditions. And respectively inoculating the skinning composition on the stone-like surfaces of the rock slopes of groups A1-A5.
A1-A3 were used as experimental groups, while A4 and A5 were used as control groups. Laying a skinning composition one on the surface of an A1-like stone; spraying a first curing composition onto the laid first skinning composition; laying a skinning composition II on the surface of the stone-like A2; spraying a curing composition II on the laid skinning composition II; laying a skinning composition III on the stone-like surface of A3; spraying a third curing composition on the third laid skinning composition; laying a skinning composition III on the stone-like surface of A4; spraying clear water with the same amount as the solidified composition III on the laid skinning composition III; placing pieces of moss in an amount equal to the weight of the skinning composition on the stone-like surface of A5; an equal amount of clear water to the curing composition is sprayed onto the dispensed moss fragments. Wherein the arrangement density of the skinning composition I, the skinning composition II and the skinning composition III is 1.5kg/m2(ii) a The spraying density of the first curing composition, the second curing composition and the third curing composition is 3L/m2。
Appropriately shading the sample stones of the A1-A5 group rock slopes, keeping the illumination at 2000-10000Lux, and culturing in an environment with the relative air humidity of more than 85% and the temperature of 20-30 ℃; and the second spraying of the curing composition was carried out on the stone-like surfaces of groups A1 to A5 on day 11/7 in 2020.
And observing the growth conditions of lichens on the surfaces of five groups A1-A5 of stone samples in 2020, 11, 15 days (18 days for inoculation), detecting and recording the indexes such as lichen shape, length, width, density and the like on various stones. Lichen coverage was measured and recorded.
FIG. 1 shows lichen growth 18 days after inoculation in the experimental group of the biological skinning composition for rock slopes according to the invention; as shown in fig. 1, when the scale is 1mm, the surface of the sample stone of the experimental group becomes visible to the naked eye and becomes significantly green 18 days after the inoculation.
The result shows that the surfaces of the A1-A3 groups of the sample stones are adhered to the surfaces of the sample stones to grow good lichens when the inoculation is carried out for 18 days, and the coverage rate is more than 70 percent; the surface coverage rate of A4 group-like stones is 40 percent; the surface coverage of the stone-like stone of group A5 was 20%.
Effect example 1
The biological skinning inoculation test was performed in the Ministry of Beijing on 28 days 10 months in 2020. Selecting sample stones 20 with flatness and similar sunshine irradiation degree on bare rock slope surface, wherein each stone is 0.5m2The stone samples are divided into five groups B1-B5, and each group contains 4 stones. The test results were averaged and tested under the same conditions. And respectively inoculating the skinning composition on the stone-like surfaces of the rock slopes of groups B1-B5.
Wherein, the lichen in the biological crust granules is a local lichen species collected from the mountain of Mentougou Lingshan of Beijing.
Fig. 2 to 3 show the conditions of experimental points in an effect example of the bio-skinning composition for a rock slope according to the present invention, wherein fig. 2 is a close-up view of the experimental points in the effect example, and fig. 3 is a perspective view of the experimental points in the effect example. As shown in the figure, the site selected by the experiment is a steep rock slope with high exposure. The rock surfaces of rock slopes of groups B1-B5 were inoculated with the skinning composition, respectively, with groups B4 and B5 serving as controls.
Arranging a first skinning composition on the surface of the B1 rock slope, and spraying a first curing composition on the arranged first skinning composition; laying a second skinning composition on the surface of the B2 rock slope, and spraying a second curing composition on the laid second skinning composition; will knotThe third skin composition is arranged on the surface of the B3 rock slope, and the third curing composition is sprayed on the third laid skinning composition; laying the third skinning composition on the surface of the B4 rock slope, and spraying clear water which is equal to the third skinning composition in quantity to the third skinning composition; arranging moss fragments in an amount equal to the weight of the skinning composition on the surface of the rock slope of B5; an equal amount of clear water to the curing composition is sprayed onto the dispensed moss fragments. Wherein the arrangement density of the skinning composition I, the skinning composition II and the skinning composition III is 1.5kg/m2(ii) a The spraying density of the first curing composition, the second curing composition and the third curing composition is 3L/m2。
And field observations were made three times at 11/19 (22 days of inoculation) and 1/2 (60 days of inoculation) at 2020. During which it experiences heavy snow and heavy wind for many times. Detecting and recording lichen shape, length, width, density, etc. on various stones. Lichen coverage was measured and recorded.
The result shows that when the inoculation is carried out for 22 days, lichens which are well grown and attached to the surfaces of the sample stones are arranged on the surfaces of the sample stones in groups B1-B3, and the coverage rate is more than 50 percent; the surface coverage rate of B4 group-like stones is only 20%; the lichen on the surface of the B5-like stone is not good enough, and the lichen on the surface of the stone-like stone is not grown.
When the inoculation is carried out for 60 days, lichens which are well grown and attached to the surfaces of the sample stones are arranged on the surfaces of the sample stones in groups B1-B3, and the coverage rate is more than 70 percent; the surface coverage rate of B4 group-like stones is only 30 percent; the lichen on the surface of the B5 group of stones has poor growth condition, and no lichen growth trace is obvious on the surface of the stones.
A fourth field observation was made at 3/2 days 2021 (4 months of inoculation). The results show that the surfaces of the B1-B3 group-like stones have lichens which are well adhered to the surfaces of the stones and grow well, the coverage rate is more than 80 percent, and the lichens all have the phenomenon of expanding and growing towards the periphery. No obvious lichen growth around the lichen was observed in groups B4 and B5.
FIGS. 4 to 7 show the growth of lichen on inoculation and after inoculation of group B3 in an effect example of the biological skinning composition for rock slopes according to the present invention; wherein, FIG. 4 is a graph showing the effect of group B3 on the same day of inoculation in the effect example of the biological skinning composition for rock slope surface of the present invention; FIG. 5 is a graph showing the effect of lichen growth in group B3 inoculated for 22 days in an example of the effect of the biological skinning composition for rocky slopes according to the present invention; FIG. 6 is a graph showing the effect of lichen growth after 4 months of inoculation of group B3 in an example of the effect of the biological skinning composition for rock slopes according to the present invention; fig. 7 is a graph showing the effect of lichen growth around 4 months after inoculation of group B3 in an example of the effect of the composition for bio-skinning on rock slopes of the present invention. As shown, the stone-like surface of group B3 had well-grown lichen already at 22 days of inoculation; after 4 months of inoculation, however, the stone-like surface of group B3 not only showed good moss growth, but also, moss growth had spread to the peripheral areas where no moss inoculation was performed.
In summary, the biological crust composition for rock slopes of the present invention, through the solidified composition containing urease-producing microorganisms, generates calcium carbonate crystals between biological crust particles and bare rocks, and further combines the crust composition and the bare rock surfaces together, so as to reduce the negative effects of wind erosion and rain erosion on lichen survival, and further achieve the effect of improving lichen survival rate.
Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements do not depart from the spirit of the invention and are intended to be included within the scope of the invention.
Claims (4)
1. The application of the biological crust composition on the rock slope is characterized in that,
laying a skinning composition prepared by mixing biological skinning particles, straw particles and a water-retaining agent according to a proportion on a rock slope;
and spraying a curing composition prepared by mixing a curing microbial agent, urea and calcium chloride according to a proportion onto the laid skinning composition.
2. The use of the biological crust composition according to claim 1 on a rock slope, wherein the biological crust particles are pieces of moss of uniform size obtained by removing moss and soil 4-10mm below it at the same time, grinding and filtering through a sieve of 3-5 mm in diameter.
3. The use of a biological skinning composition according to claim 1 on rock slopes,
the manufacturing method of the straw particles comprises the following steps:
crushing the straws into straw fragments of 3-5 cm;
soaking the straw fragments for 24-72 hours, and then drying to control the water content to be 20%;
making the dried straw fragments into straw particles with the fineness of 0.1 mm.
4. The use of the biological crust composition according to claim 1 on a rock slope, wherein the solidified microbial agent is prepared by a method comprising:
inoculating Papanicolaou bacillus to Papanicolaou bacillus culture medium solution, wherein the Papanicolaou bacillus culture medium solution comprises 10g/L tryptone, 5g/L beef extract, 6.5g/L yeast extract, 5g/L, NaCl1 g/1 g/L ammonium sulfate and H2A culture medium of O1L; adding urea to the culture medium solution to reach the concentration of 10g/L, and adding NiCl2To a concentration of 4 g/L; culturing and diluting the bacillus pasteurianus liquid at the rotation speed of 130rmp/min and the OD600 of 0.7-0.9 under the conditions of 31 ℃ and pH of 8.1;
inoculating Bacillus licheniformis to Bacillus licheniformis culture medium solution, wherein the Bacillus licheniformis culture medium solution comprises tryptone 10g/L, beef extract 5g/L, yeast extract 6.5g/L, ammonium sulfate 5g/L, NaCl1g/L and H2A culture medium of O1L; adding urea to the culture medium solution to reach the concentration of 10g/L, and adding NiCl2To a concentration of 4 g/L; culturing and diluting the bacillus licheniformis liquid at 31 ℃ and pH 8.1 with the rotating speed of a shaking table of 130rmp/min until OD600 is 0.7-0.9;
and preparing the bacillus pasteurianus bacterial liquid and the bacillus licheniformis bacterial liquid into a solidified microbial agent according to the volume ratio of 3-5: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111038548.1A CN113943581A (en) | 2021-09-06 | 2021-09-06 | Application of biological skinning composition to rock slope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111038548.1A CN113943581A (en) | 2021-09-06 | 2021-09-06 | Application of biological skinning composition to rock slope |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113943581A true CN113943581A (en) | 2022-01-18 |
Family
ID=79328078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111038548.1A Pending CN113943581A (en) | 2021-09-06 | 2021-09-06 | Application of biological skinning composition to rock slope |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113943581A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115492077A (en) * | 2022-11-15 | 2022-12-20 | 南京大学 | Method for protecting rainwater erosion on surface of rammed great wall by utilizing algae and urease-producing bacteria |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080245272A1 (en) * | 2004-12-20 | 2008-10-09 | Kucharski Edward S | Microbial Biocementation |
| CN102138508A (en) * | 2011-03-22 | 2011-08-03 | 北京市农林科学院 | Method for greening and protecting steep road side slope by using biological crust |
| CN102204434A (en) * | 2011-03-25 | 2011-10-05 | 北京市农林科学院 | Method for preventing water erosion desertification by using biological crust |
| CN106699026A (en) * | 2016-12-02 | 2017-05-24 | 太原理工大学 | Crack self-remediation regenerated concrete based on urease production microorganism mineralization deposition and preparation method |
| CN109095977A (en) * | 2017-06-20 | 2018-12-28 | 黑龙江省水利科学研究院 | A kind of preparation method of straw pulp biological mulch film |
| CN109122164A (en) * | 2018-07-18 | 2019-01-04 | 南京林业大学 | A method of it is covered using moss progress rock green |
| CN109706944A (en) * | 2019-01-09 | 2019-05-03 | 安徽金联地矿科技有限公司 | Moss for high steep rock slope answers method for green |
| CN111254946A (en) * | 2020-01-16 | 2020-06-09 | 西北农林科技大学 | Method for reinforcing and protecting loess slope surface based on microbial mineralization |
| CN111557220A (en) * | 2020-04-16 | 2020-08-21 | 西北农林科技大学 | Rapid recovery method for field sandy moss crust |
-
2021
- 2021-09-06 CN CN202111038548.1A patent/CN113943581A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080245272A1 (en) * | 2004-12-20 | 2008-10-09 | Kucharski Edward S | Microbial Biocementation |
| CN102138508A (en) * | 2011-03-22 | 2011-08-03 | 北京市农林科学院 | Method for greening and protecting steep road side slope by using biological crust |
| CN102204434A (en) * | 2011-03-25 | 2011-10-05 | 北京市农林科学院 | Method for preventing water erosion desertification by using biological crust |
| CN106699026A (en) * | 2016-12-02 | 2017-05-24 | 太原理工大学 | Crack self-remediation regenerated concrete based on urease production microorganism mineralization deposition and preparation method |
| CN109095977A (en) * | 2017-06-20 | 2018-12-28 | 黑龙江省水利科学研究院 | A kind of preparation method of straw pulp biological mulch film |
| CN109122164A (en) * | 2018-07-18 | 2019-01-04 | 南京林业大学 | A method of it is covered using moss progress rock green |
| CN109706944A (en) * | 2019-01-09 | 2019-05-03 | 安徽金联地矿科技有限公司 | Moss for high steep rock slope answers method for green |
| CN111254946A (en) * | 2020-01-16 | 2020-06-09 | 西北农林科技大学 | Method for reinforcing and protecting loess slope surface based on microbial mineralization |
| CN111557220A (en) * | 2020-04-16 | 2020-08-21 | 西北农林科技大学 | Rapid recovery method for field sandy moss crust |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115492077A (en) * | 2022-11-15 | 2022-12-20 | 南京大学 | Method for protecting rainwater erosion on surface of rammed great wall by utilizing algae and urease-producing bacteria |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108353563B (en) | Method for treating desertified soil by using wastes as soil conditioner | |
| CN111117638B (en) | Soda saline-alkali soil modifier and modification method | |
| Wakefield et al. | An introduction to stone colonizing micro-organisms and biodeterioration of building stone | |
| CN107853093B (en) | Biological modifier for beach saline-alkali soil and preparation method and application thereof | |
| KR101543134B1 (en) | Method for producing thalli of lichens, method for restoring the degraded ecology by them, and compositions therefor | |
| CN108570993B (en) | Ecological vegetation concrete slope protection structure and construction method thereof | |
| CN102612953B (en) | Pattern for building artificial algal crust in desert | |
| Isichei | The role of algae and cyanobacteria in arid lands. A review | |
| CN111316781A (en) | Planting method for ecological crust of vegetation degraded bare land | |
| CN112209778A (en) | Novel slag soil conditioner and preparation method thereof | |
| CN113943581A (en) | Application of biological skinning composition to rock slope | |
| CN112854250B (en) | Method for solidifying road slope soil by combining microorganisms and plants | |
| CN114672420A (en) | Method for quickly repairing mine in fragile habitat area | |
| CN113906854B (en) | Method for treating saline-alkali soil in arid region by utilizing artificial composite biological crust | |
| CN113773156A (en) | Biological crust composition for rock slope | |
| CN111057556A (en) | Coastal saline-alkali soil improvement agent and improvement method | |
| CN106242626A (en) | A kind of environment-friendly type plant pit covering | |
| CN113287382B (en) | Algal fungus skinning cultivation method suitable for calcareous sandy soil of island reef of south China sea coral | |
| KR20120009559A (en) | Method for restoring the degraded ecology by lichen and compositions therefor | |
| Sáiz-Jiménez | Weathering and colonization of limestones in an urban environment | |
| Li et al. | Cryptogam diversity and formation of soil crusts in temperate desert | |
| CN111117892B (en) | Green alga Auxenochlorella sp.BSC-01 and application thereof | |
| CN115247132B (en) | Method for controlling desert by combined artificial biological crust | |
| CN114027111B (en) | Method for ecologically treating high-steep slope with acid rock covered by dicranopteris pedata layer | |
| CN114568252B (en) | Matrix material for repairing mine rock slope and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |