CN106631560A - Microorganism type pest-resistant fertilizer carrier and preparation method thereof - Google Patents

Abstract

The invention discloses a microorganism type pest-resistant fertilizer carrier and a preparation method thereof. The microorganism type pest-resistant fertilizer carrier is prepared from the following raw materials in parts by weight: an acrylic acid solution, N,N-methylene bisacrylamide, starch, a hybrid entomogenous fungus powder, carboxymethylcellulose, cassava waste, sour milk, maltose, sodium bicarbonate, potato peels, wheat bran, blast furnace coal slag, bamboo charcoal, bamboo vinegar, chitosan, powdered rock phosphate and a proper amount of water. The microorganism type pest-resistant fertilizer carrier is a carrier for fertilizer, can be used as an additive for all types of fertilizer, accounts for 1/3 to 1/2 of the fertilizer, has the effects of killing and resisting pests, is obvious in acting effect and high in efficiency; the entomogenous fungus serving as a microbial insect killing component is introduced, and an original strain is selected from strains retained in an efficiency laboratory, so that dry microorganism powder prepared through enlarged culture has an effect of killing pests.

Description

A kind of microbiocidal insect pest fertilizer carrier and preparation method thereof

Technical field

The invention belongs to the technology field of fertilizer, and in particular to a kind of microbiocidal insect pest fertilizer carrier and its Preparation method.

Background technology

Entomogenous fungi plays the role of particularly important in the biological control of insect, and their species is various, metabolic type Complexity, without entomogenous fungi is not contained from Mastigomycotina to Deuteromycotina, is related to altogether about 100 category, and more than 800 plant, in recent years, by In insect pathogenic fungus in the prospect of integrated pest control and its metabolite application more being subject to people's attention, using chemistry The pest and disease damage of pesticide control agricultural, greatly destroys the ecological balance, and the value volume and range of product of insect rises one after another, the resistance to the action of a drug Also great rising, while secondary insect is changed into main insect, the frequent of agricultural chemicals cause severe contamination, agriculture to environment using meeting The agricultural chemicals remained in product can be detrimental to health.

Dipel found in 1901 by Japanese scholars, and because producing insecticidal proteins squama wing is widely used in The preventing and treating of the agriculture and forestry injurious insects such as mesh, Diptera, the Bt bacterial strains reported in recent years to some Hymenopteras, Orthoptera, Mallophaga insect with And nematode, mite, protozoan also have an insecticidal activity, Dipel be in the world the most commonly used microbial pesticide of purposes it One, with instant effect, killing rate is high, it is harmless to the mankind the advantages of, for preventing and treating crop, vegetables, cotton, soybean, tobacco, fruit tree Etc. every aspect；Its main active insecticidal components is parasporal crystal, a kind of crystalline protein or endotoxin, and parasporal crystal is by insect After eating, in the presence of the alkaline environment and protease of middle intestinesδ- endotoxin is decomposed and activation, becomes with insecticidal activity Toxic peptide.Toxic peptide and the single-minded receptor binding of larva intestinal epithelial cell membrane, cause film to be bored a hole, and enteron aisle Dissolve things inside penetrates into blood Chamber, while gemma takes advantage of the occasion to invade propagation, causes and be enough to lethal septicemia, causes insect general paralysis or spasm and dead.

Beauveria, can parasitic 15 mesh, 149 sections, more than 700 kinds of 521 category in Hypocreales Chinese caterpillar fungus Cordycepps Cordyceps 6 sections of insect and Acarina, 17 kinds of mites and tick.Research shows that muscardine has certain prevention effect to orange grizzled bar longicorn, To the corrected mortality of larva up to 71. 05%.Muscardine is studied in the world and using the wide Insect Pathogenic of most, parasitic scope One of fungi, have the advantages that high selectivity, safe noresidue, without the resistance to the action of a drug, repeatedly infect, it is compatible good.

The prevention and control of plant diseases, pest control being used in terms of agricultural product with entomogenous fungi is following leading advanced prevention and elimination of disease and pests technology, The features such as with security, novelty and creativeness, but the class scope of entomogenous fungi is less, the active and ring of this kind of fungi Border adaptability is as common micro-organisms, it is necessary in good living environment, the maximum effectiveness of competence exertion, applies to fertilizer Material is rarely found, and the chemical element in fertilizer can significantly change the physicochemical environment of microorganism, very big to the survival effect of microorganism, because How this improves critically important for the entomogenous fungi quantity and living environment that is active, improving entomogenous fungi of insect growth.

The content of the invention

In order to improve the diversity of fertilizer so as to which, with certain insect resistace, entomogenous fungi is introduced the life of fertilizer for invention Produce, it is leading filler that a kind of entomogenous fungi containing efficient anti-insect activity is added in fertilizer preparation process, is specifically led to Cross what following method was realized：

A kind of microbiocidal insect pest fertilizer carrier, it is characterised in that be made up of the raw material of following weight portion：Acrylic acid solution 15 ~ 25, N,N methylene bis acrylamide 0.2 ~ 0.5, starch 5 ~ 8, mixing entomogenous fungi bacterium powder 3 ~ 5, carboxymethylcellulose calcium 2 ~ 4, Manioc waste 20 ~ 30, rotten milk 3 ~ 6, maltose 4 ~ 7, sodium bicarbonate 0.5 ~ 1.0, potato peel 5 ~ 9, wheat bran 10 ~ 15, blast furnace Cinder 15 ~ 25, bamboo charcoal 2 ~ 5, bamboo vinegar 5 ~ 8, shitosan 1 ~ 3, ground phosphate rock 30 ~ 50 and appropriate water；

The mixing entomogenous fungi bacterium powder is Dipel original powder and conidia of beauveria bassiana powder according to 3:1 ratio mixing institute .

A kind of microbiocidal insect pest fertilizer carrier preparation method, it is characterised in that including following step：

（1）Airing, water content 40 ~ 50%, then by the powder of ground 80 ~ 120 mesh sieve of wheat bran are carried out to manioc waste spray water End, the water for mixing potato peel and 10 ~ 15 times of its total amounts boils, and adds carboxymethylcellulose calcium, rotten milk, maltose and little Su Beat, 10 ~ 15min of holding is boiled again, be uniformly cast on aforesaid manioc waste, standing solidifies out into culture medium；

（2）Water mixed diluting of the entomogenous fungi bacterium powder with 10 ~ 15 times will be mixed, be then sprayed onto（1）On described culture medium, lead to 25 ~ 30 DEG C of 70 ~ 80h of culture grow mycelium and bulk bacterium colony under the conditions of wind, microorganism are then dried and made in 60 ~ 80 DEG C and is done Powder；

（3）Starch is mixed with the water of 8 ~ 10 times of its weight portions, 80 ~ 90 DEG C of stirring gelatinizations are heated to, the mode being then added dropwise is delayed It is slow to add acrylic acid solution, 60 ~ 70 DEG C of 3.0 ~ 3.5h of reaction are incubated, N is eventually adding, the crosslinking of N- methylene-bisacrylamides is dried Solidization, grinds to form pulvis；

（4）Mixing blast furnace cinder, ground phosphate rock, 2 ~ 3h of high-temperature calcination is added after natural cooling（2）The dry powder and（3）Gained powder Agent and other residual components, mix.

Chemotrophy element Acidity of Aikalinity and other side property affect the existence of microorganism, invention to cause in order to prevent fertilizer , in microorganism entomogenous fungi is made into a kind of pulvis, the existence of microorganism must be under conditions of water environment, the fertilizer of dryness for power Material is easily caused microorganism dehydration and dead, largely effects on survival rate, and is unfavorable for quantitative accumulation, therefore, will be required It is best selection that entomogenous fungi makes pulvis, and at this moment microorganism in a dormant state, can recover again under suitable environment Activity.

Moreover, the main fungal bacterial classification that the present invention is adopted first passes around expansion training for muscardine and Dipel Support, for required bacterial classification the advantage in quantity and activity is purposefully improved, moreover, invention provides a kind of to form sediment The resin material of powder grafting is accole, and the monomer of resin material is mainly acrylic monomers, is a kind of degradable resinous wood Material, security performance is high, coordinates appropriate crosslinking agent, has fabulous water-absorbing-retaining property in soil, using substantial amounts of starch Stable performance after grafting materials, it is more topmost to be：Predominantly carbon source and the nitrogen source of the living necessities of entomogenous fungi, adds Starch can be utilized by microbial metabolism, there is many places hole in the resin powdery microparticle surfaces for making preparation, surface area increase, plus Big water suction and water retention characteristic, hole provides microorganism good living environment simultaneously, improves the activity of microorganism, improves efficiency, In the form of a kind of accole.

Starch is a kind of special natural organic matter, rich reserves, and substantial amounts of hydroxyl can be with hydrone shape on its strand Into hydrogen bond, with great hydrophilic and water suction ability, invention is proved by way of test：First by starch and 10 times or so Water mixing, be heated to 80 ~ 90 DEG C gelatinization 1.0 ~ 1.5h, after stirring be incubated 60 DEG C or so insulation, add acrylic monomers solution, 3 ~ 4h is reacted under heat-retaining condition, crosslinking agent N is subsequently adding, N- methylene-bisacrylamides can improve into plasticity, tie resin Structure is the network structure in space, strengthens performance, then stands baking and curing, is finally ground into pulvis；Then according to microorganism Growth conditions and habit add required nutrient and each side nutritional condition, make microbiological culture media, while being inoculated with muscardine Growth, the cultivation through 15 ~ 20 days finds that the dry weight of culture medium is reduced, and the quantity of muscardine is lifted, while using culture super Sonication obtains the minimum resin particle shape thing of granularity after cleaning, and shows under high-power microscope：Rough, and not Using difference after the resin particle contrast of degraded substantially, there are many holes and with cracking sign, while also similar stone heap The lamellar projection of cumuliformis, particle is loose porous, easily crushes again, it was demonstrated that the microorganism starch used to grafting is dropped Solution, the resin particle of porous improves the retain water and nutrients ability of soil, while the excellent environment of effect entomogenous fungi can be provided, Play the purpose of biological pest control.

Beneficial effects of the present invention：The invention belongs to a kind of carrier of fertilizer, can be used for the additive of all kinds of fertilizer, occupy The 1/3 ~ 1/2 of fertilizer ratio, with the pest-resistant effect of desinsection, substantially, efficiency high introduces microbial source insecticidal constituent worm to action effect Raw fungi, opportunistic pathogen kind takes from efficient laboratory and retains bacterial classification, is enlarged after culture fruit and makes microbial dry powder, has to insect Killing action.

Specific embodiment

Embodiment 1：

A kind of microbiocidal insect pest fertilizer carrier, it is characterised in that be made up of the raw material of following weight portion (Kg)：Acrylic acid is molten Liquid 20, N,N methylene bis acrylamide 0.3, starch 7, mixing entomogenous fungi bacterium powder 4, carboxymethylcellulose calcium 2, manioc waste 24, Rotten milk 4, maltose 6, sodium bicarbonate 0.8, potato peel 7, wheat bran 12, blast furnace cinder 20, bamboo charcoal 3, bamboo vinegar 7, shell gather Sugar 2, ground phosphate rock 40 and appropriate water；

The mixing entomogenous fungi bacterium powder is Dipel original powder and conidia of beauveria bassiana powder according to 3:1 ratio mixing institute .

A kind of microbiocidal insect pest fertilizer carrier preparation method, it is characterised in that including following step：

（1）Airing, water content 40 ~ 50%, then by the powder of ground 80 ~ 120 mesh sieve of wheat bran are carried out to manioc waste spray water End, the water for mixing potato peel and 10 ~ 15 times of its total amounts boils, and adds carboxymethylcellulose calcium, rotten milk, maltose and little Su Beat, 10 ~ 15min of holding is boiled again, be uniformly cast on aforesaid manioc waste, standing solidifies out into culture medium；

（2）Water mixed diluting of the entomogenous fungi bacterium powder with 10 ~ 15 times will be mixed, be then sprayed onto（1）On described culture medium, lead to 25 ~ 30 DEG C of 70 ~ 80h of culture grow mycelium and bulk bacterium colony under the conditions of wind, microorganism are then dried and made in 60 ~ 80 DEG C and is done Powder；

（3）Starch is mixed with the water of 8 ~ 10 times of its weight portions, 80 ~ 90 DEG C of stirring gelatinizations are heated to, the mode being then added dropwise is delayed It is slow to add acrylic acid solution, 60 ~ 70 DEG C of 3.0 ~ 3.5h of reaction are incubated, N is eventually adding, the crosslinking of N- methylene-bisacrylamides is dried Solidization, grinds to form pulvis；

（4）Mixing blast furnace cinder, ground phosphate rock, 2 ~ 3h of high-temperature calcination is added after natural cooling（2）The dry powder and（3）Gained powder Agent and other residual components, mix.

The carrier of the present invention is used for into composite fertilizer, molten condition will be heated to after nutrient inorganic elements mixing needed for crop, The carrier for being subsequently adding the present invention mixes as inserts, and granulation becomes a kind of powdered fertilizer particle, and the experiment of plantation is proved： The fertilizer for preparing in this way can greatly improve composite fertilizer's fertilizer efficiency, improve soil moisturizing ability, the incidence drop of pest and disease damage It is low.

Claims (2)

1. a kind of microbiocidal insect pest fertilizer carrier, it is characterised in that be made up of the raw material of following weight portion：Acrylic acid solution 15 ~ 25, N,N methylene bis acrylamide 0.2 ~ 0.5, starch 5 ~ 8, mixing entomogenous fungi bacterium powder 3 ~ 5, carboxymethylcellulose calcium 2 ~ 4th, manioc waste 20 ~ 30, rotten milk 3 ~ 6, maltose 4 ~ 7, sodium bicarbonate 0.5 ~ 1.0, potato peel 5 ~ 9, wheat bran 10 ~ 15, height Stove cinder 15 ~ 25, bamboo charcoal 2 ~ 5, bamboo vinegar 5 ~ 8, shitosan 1 ~ 3, ground phosphate rock 30 ~ 50 and appropriate water；

The mixing entomogenous fungi bacterium powder is Dipel original powder and conidia of beauveria bassiana powder according to 3:1 ratio mixing institute .

2. a kind of microbiocidal insect pest fertilizer carrier preparation method according to claim 1, it is characterised in that include with Under several steps：

（1）Airing, water content 40 ~ 50%, then by the powder of ground 80 ~ 120 mesh sieve of wheat bran are carried out to manioc waste spray water End, the water for mixing potato peel and 10 ~ 15 times of its total amounts boils, and adds carboxymethylcellulose calcium, rotten milk, maltose and little Su Beat, 10 ~ 15min of holding is boiled again, be uniformly cast on aforesaid manioc waste, standing solidifies out into culture medium；

（2）Water mixed diluting of the entomogenous fungi bacterium powder with 10 ~ 15 times will be mixed, be then sprayed onto（1）On described culture medium, lead to 25 ~ 30 DEG C of 70 ~ 80h of culture grow mycelium and bulk bacterium colony under the conditions of wind, microorganism are then dried and made in 60 ~ 80 DEG C and is done Powder；

（3）Starch is mixed with the water of 8 ~ 10 times of its weight portions, 80 ~ 90 DEG C of stirring gelatinizations are heated to, the mode being then added dropwise is delayed It is slow to add acrylic acid solution, 60 ~ 70 DEG C of 3.0 ~ 3.5h of reaction are incubated, N is eventually adding, the crosslinking of N- methylene-bisacrylamides is dried Solidization, grinds to form pulvis；

（4）Mixing blast furnace cinder, ground phosphate rock, 2 ~ 3h of high-temperature calcination is added after natural cooling（2）The dry powder and（3）Gained powder Agent and other residual components, mix.