CN101565688B - Trichodrema spp. microbial agent and preparation method thereof - Google Patents

Trichodrema spp. microbial agent and preparation method thereof Download PDF

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Publication number
CN101565688B
CN101565688B CN2008100111865A CN200810011186A CN101565688B CN 101565688 B CN101565688 B CN 101565688B CN 2008100111865 A CN2008100111865 A CN 2008100111865A CN 200810011186 A CN200810011186 A CN 200810011186A CN 101565688 B CN101565688 B CN 101565688B
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wood
trichoderma
mixing raw
auxiliary
mould spore
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CN101565688A (en
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张惠文
阮晓东
蔡颖慧
张晓黎
罗永平
苏振成
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Institute of Applied Ecology of CAS
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Institute of Applied Ecology of CAS
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Abstract

The invention relates to a biological preparation, in particular to a Trichodrema spp. microbial agent and a preparation method thereof. The method comprises the following steps: the dried and crushed8-9 edible fungus chaff according to parts by weight is mixed with 2-4 auxiliary material according to parts by weight, the acidity-alkalinity pH of the mixed raw material is adjusted to 5.0-6.5, then the Trichodrema spp. suspension with 5-10 percent of mass ratio with the mixed raw material is inoculated to the mixed raw material and cultured for 7-10 days at the temperature of 8-30 DEG C, and after the Trichodrema spp. is generated, the well cultured material is dried and crushed to obtain Trichodrema spp. microbial agent. The invention takes the treated edible fungus chaff as the raw material to produce the Trichodrema spp. microbial agent, fully utilizes the rest nutritious substance in the edible fungus chaff as well as the characteristics of loose and permeability of the fungus chaff, promotes the value in use of the fungus chaff of the edible fungus, and simultaneously reduces the production cost of the Trichodrema spp. microbial agent, the produced Trichodrema spp. is mainly chlamydospore, the multiplication capacity and the colonizing capacity of the Trichodrema spp. in the microbial agent are enhanced, the capability of resisting the unfavorable environmental conditions is also enhanced and the survival time of the Trichodrema spp. is prolonged.

Description

Mould spore microbial inoculum of a kind of wood and preparation method thereof
Technical field
The present invention relates to biotechnological formulation, be specifically related to mould spore microbial inoculum of a kind of wood and preparation method thereof.
Background technology
Trichoderma (Trichoderma spp.) belongs to Deuteromycotina, Haplomycetes, Hyphomycetales, and sticking spore mushroom is the ubiquitous class fungi of occurring in nature, encloses and seed, bulb surface often can be separated at soil, plant rhizosphere, leaf.Trichoderma is the antagonism fungi that a class has the important biomolecule control to be worth, and can prevent and treat fungal disease as trichoderma harziarum (Trichoderma harzianum), hook-shaped wood mould (Trichodermahamatum), viride (Trichoderma viride).Clear and definite now its mechanism of action comprises microbiotic, hyperparasitism, bacteriolysis, the competition effect etc. of producing.In addition, wood is mould to also have stimulating plant to grow, and increases the effect of output.Test shows, the trichoderma as biological preparation has good prevention effect to the samping off of many farm crop, vegetables, flowers, tobacco, damping-off, southern blight, balck shank, root rot, oidium, red-star like disease etc.It is safety, free of contamination Prevention Technique that application trichoderma as biological preparation is prevented and treated soil-borne disease.Trichoderma can produce three kinds of propaguluies in its growth cycle, comprise mycelium, chlamydospore and conidium.Produce Trichoderma agent upward commonly used at present and mostly be its conidium preparation alive, as the trichoderma harziarum T39 wettable powder Trichodex of Israel's exploitation, the Topshield of the U.S. (trichoderma harziarum T22) etc.But the conidium preparation is subject to the interference of extraneous various factors, border mensuration conidium survival time had only about half a year factually, conidium is relatively poor in field sprouting and adaptability simultaneously, ecotope is had relatively high expectations, thereby mould propagation and the colonization ability in soil of influence wood causes the active decline of its biological and ecological methods to prevent plant disease, pests, and erosion.Chlamydospore is that son is embraced in the mould a kind of dormancy that produces under adverse environmental factor of wood, also is the important propagulum form of Trichoderma.The Trichoderma chlamydospore have anti-drying, low temperature resistant, to advantages such as soil fungistasis are insensitive, and Trichoderma chlamydospore survival ability in soil is better than conidium, can survive at least 20 months, thick wall is embraced son, and to sprout speed also fast than conidium.Therefore cultivate chlamydospore making biocontrol fungicide and become problem demanding prompt solution.
Edible fungus bran is a waste remaining after the culturing edible fungus class.The main matrix of edible fungus culturing raw material has corn cob, cotton seed hulls, wood sawdust and various crop stalk and industrial waste.After cultivation, most of easily material of decomposition and inversion bacterium that has been eaten in the course of cultivation absorbs in the raw material, but still contains abundant tropina and other nutritive substance in the remaining bacterium chaff, has higher utility value.If these edible fungus brans are arbitrarily abandoned or are stacked, not only wasted valuable resource, also can land occupation, cause mould and insect to grow, polluted the environment in edible mushrooms producing region.
Summary of the invention
It is good to the purpose of this invention is to provide a kind of suitability, and technology is simple, wooden mould spore microbial inoculum that is easy to grasp and preparation method thereof.
For achieving the above object, the technical solution used in the present invention is:
The mould spore microbial inoculum of wood: the 8-9 of meter by weight part edible fungus bran of crushed after being dried is mixed with 2-4 part auxiliary material, regulating mixing raw material potential of hydrogen PH is 5.0-6.5, to be that the wooden mould spore suspension of 5%-10% is inoculated on the mixing raw material then with the mixing raw material mass ratio, at relative humidity 60-80%, temperature 25-30 ℃, cultivate after 4-6 days under the no optical condition, 8-12 ℃ of low temperature do not have under the optical condition cultivate treated the mould spore of output wood in 3-4 days after, with cultured dry materials, pulverize to be and produce wooden mould spore microbial inoculum;
Described auxiliary material is counted Semen Maydis powder 1-2 part by weight, wheat bran 1-1.5 part; Potassium primary phosphate 0.001-0.020 part, sal epsom 0.001-0.020 part, compound little vitamins B 0.001-0.020 part; Or sweet potato powder 1-2 part, dried silkworm chrysalis meal 1-2 part; Potassium primary phosphate 0.001-0.020 part, sal epsom 0.001-0.020 part, compound little vitamins B 0.001-0.020 part.
Wooden mould bacterial classification in the mould spore suspension of described wood is one or more in viride (Trichodermaviride), trichoderma harziarum (Trichoderma harzianum), hook-shaped wood mould (Trichodermahamatum), yellow green trichoderma (Trichoderma aureoviride) and the tangerine green trichoderma (Trichodermacitrinoriride), presses arbitrary proportion compound fungi strain.Edible fungus bran is produced the substratum of edible mushrooms and is made up of major ingredient and auxiliary material for producing the substratum behind the edible mushrooms according to a conventional method, content meter by weight, and wherein major ingredient accounts for 92-98%, and auxiliary material accounts for 2-8%; Major ingredient is by cotton seed hulls, wood sawdust, straw, corn cob, bagasse, corn stalk powder, soya bean stem meal, wheat bran, rice chaff, rice husk, cotton straw powder, wheat straw, weeds, and bagasse, vinasse, vinegar are poor, 2~4 kinds of mixtures in xylose residue, rapeseed cake, pig manure, chicken manure, horsehit, cow dung, human excrement, pigsty fertilizer and the pond sludge soil are formed; Auxiliary material is made up of the 3-6 kind mixture in soybean cake powder, cotton seed cake, wheat bran, urea, composite fertilizer, calcium superphosphate, fused(calcium magnesium)phosphate, lime powder, terra alba, lime carbonate, plant ash, potassium primary phosphate, sucrose, brown sugar, ammoniacal liquor, dried silkworm chrysalis meal, glucose and the VITMAIN B1; The edible fungus bran mycelia is pure white, the material agllutination is real, nothing is gone mouldy and rotten composition the C of bacterium chaff: N=20-35: 1, and C: P=120-270: 1, the bacterium chaff water content 10-25% of drying, the bacterium chaff particle diameter 0.2-1.0cm of pulverizing.The water that adds mixing raw material weight 70%-120% in the described mixing raw material; The potential of hydrogen of described mixing raw material adopts hydrochloric acid and lime powder to regulate.The wooden mould spore suspension miospore quantity of described inoculation is 10 5-10 9Individual/ml; Described mixing raw material is packed in high-temperature resistance plastice bag or the fermenting container, 115 ℃-121 ℃ of following moist heat sterilization 20-60 minutes.
The preparation method of the mould spore microbial inoculum of wood: the 8-9 of meter by weight part edible fungus bran of crushed after being dried is mixed with 2-4 part auxiliary material, regulating mixing raw material potential of hydrogen PH is 5.0-6.5, to be that the wooden mould spore suspension of 5%-10% is inoculated on the mixing raw material at relative humidity 60-80% then with the mixing raw material mass ratio, temperature 25-30 ℃, cultivate after 4-6 days under the no optical condition, 8-12 ℃ of low temperature do not have under the optical condition cultivate treated the mould spore of output wood in 3-4 days after, with cultured dry materials, pulverize to be and produce wooden mould spore microbial inoculum;
Described auxiliary material is counted Semen Maydis powder 1-2 part by weight, wheat bran 1-1.5 part; Potassium primary phosphate 0.001-0.020 part, sal epsom 0.001-0.020 part, compound little vitamins B 0.001-0.020 part; Or sweet potato powder 1-2 part, dried silkworm chrysalis meal 1-2 part; Potassium primary phosphate 0.001-0.020 part, sal epsom 0.001-0.020 part, compound little vitamins B 0.001-0.020 part.
Wooden mould bacterial classification in the mould spore suspension of described wood is one or more in viride (Trichodermaviride), trichoderma harziarum (Trichoderma harzianum), hook-shaped wood mould (Trichodermahamatum), yellow green trichoderma (Trichoderma aureoviride) and the tangerine green trichoderma (Trichodermacitrinoriride), presses arbitrary proportion compound fungi strain.Edible fungus bran is produced the substratum of edible mushrooms and is made up of major ingredient and auxiliary material for producing the substratum behind the edible mushrooms according to a conventional method, content meter by weight, and wherein major ingredient accounts for 92-98%, and auxiliary material accounts for 2-8%; Major ingredient is by cotton seed hulls, wood sawdust, straw, corn cob, bagasse, corn stalk powder, soya bean stem meal, wheat bran, rice chaff, rice husk, cotton straw powder, wheat straw, weeds, and bagasse, vinasse, vinegar are poor, 2~4 kinds of mixtures in xylose residue, rapeseed cake, pig manure, chicken manure, horsehit, cow dung, human excrement, pigsty fertilizer and the pond sludge soil are formed; Auxiliary material is made up of the 3-6 kind mixture in soybean cake powder, cotton seed cake, wheat bran, urea, composite fertilizer, calcium superphosphate, fused(calcium magnesium)phosphate, lime powder, terra alba, lime carbonate, plant ash, potassium primary phosphate, sucrose, brown sugar, ammoniacal liquor, dried silkworm chrysalis meal, glucose and the VITMAIN B1; The edible fungus bran mycelia is pure white, the material agllutination is real, nothing is gone mouldy and rotten composition the C of bacterium chaff: N=20-35: 1, and C: P=120-270: 1, the bacterium chaff water content 10-25% of drying, the bacterium chaff particle diameter 0.2-1.0cm of pulverizing.The water that adds mixing raw material weight 70%-120% in the described mixing raw material; The potential of hydrogen of described mixing raw material adopts hydrochloric acid or lime powder to advance to regulate.The wooden mould spore suspension miospore quantity of described inoculation is 10 5-10 9Individual/ml; Described mixing raw material is packed in high-temperature resistance plastice bag or the fermenting container, 115 ℃-121 ℃ of following moist heat sterilization 20-60 minutes.
The advantage that the present invention had:
1. the present invention produces wooden mould spore microbial inoculum through the method that the blended solid with auxiliary material ferments with the bacterium chaff after dry and pulverizing, remaining nutritive substance and the loose gas-pervious character of bacterium chaff in the edible fungus bran have been made full use of on the one hand, the wooden mould spore type of producing on the other hand is mainly chlamydospore, make propagation and the colonization ability raising of wooden mould spore in soil in the microbial inoculum, opposing unsuitable environmental condition ability strengthens, and the mould survival time of wood prolongs.
2. the present invention only need be fermented and can finish by a spot of auxiliary material on the edible fungus bran basis, need not add other material, can reduce the manufacturing cost of wooden mould spore microbial inoculum, has considerable economic.
3. the present invention adopts edible fungus bran to produce wooden mould spore microbial inoculum as raw material, both solved the edible mushrooms producing region because the bacterium chaff is stacked the problem of environmental pollution that causes, utilized natural resources again preferably,, expanded the industrial chain of edible mushrooms for the production of the mould spore of wood provides good culture medium.
Embodiment
Embodiment 1
Present embodiment adopts and consists of cotton seed hulls 80%, rapeseed cake 15%, terra alba 2%, calcium superphosphate 2%, the culture medium waste flat mushroom bacterium chaff behind the production flat mushroom of urea 1%.Choose that mycelia is pure white, the material agllutination is real, do not have and go mouldy and the bar-shaped flat mushroom bacterium chaff of rotten composition, the plastics bag film that the bacterium bag is outer is sloughed, and spreads out and takes daylight drying in the sun method to make bacterium chaff drying, and dry back bacterium chaff water content is 10%-15%.Dried bacterium chaff adopts pulverizer to smash, and the powdery bacterium chaff maximum diameter after the processing is 0.20cm.
8 parts of edible fungus brans of meter by weight of crushed after being dried are mixed with 2.035 parts of auxiliary materials, the water that in mixing raw material, adds mixing raw material gross weight 80%, stir, adopting hydrochloric acid and the lime powder adjusting mixing raw material potential of hydrogen PH of 1M is 6.0, the mixing raw material that regulates is packed in the high temperature resistant eggplant-shape bottle, 121 ℃ of following moist heat sterilizations 30 minutes, treat that the solid medium temperature reduces to room temperature, be that 10% viride (Trichoderma viride) spore suspension is inoculated on the mixing raw material then with the mixing raw material mass ratio, in relative humidity 60%, 28 ℃ of temperature, cultivate after 5 days under the no optical condition, do not have under the optical condition at 10 ℃ of low temperature and to cultivate 3 days culture materials and all be green, behind the mould spore of output wood.Fermentation ends, cultured material is air-dry with sterile air under 25-35 ℃, pulverizing makes particle diameter at 0.30-0.60mm, crosses 40 mesh sieves, promptly gets wooden mould spore microbial inoculum, and viride spore type is mainly chlamydospore in the microbial inoculum, and quantity reaches 1.0-1.4 * 10 9Individual/the gram microbial inoculum.
Flat mushroom bacterium chaff mixes with auxiliary material: adopt artificial turning method that flat mushroom bacterium chaff is counted by weight with auxiliary material and mix, 8 parts of bacterium chaffs, 1 part of Semen Maydis powder, 1 part in wheat bran, 0.01 part of potassium primary phosphate, 0.01 part in sal epsom, 0.015 part of compound little vitamins B.Viride spore suspension miospore quantity is 10 7Individual/ml.
Embodiment 2
Present embodiment adopts and consists of rice chaff 35%, bagasse 35%, wheat bran 25%, urea 1%, cotton seed cake 2%, the culture medium waste golden mushroom chaff behind the production golden mushroom of lime powder 1% and lime carbonate 1%.Choose that mycelia is pure white, the material agllutination is real, do not have and go mouldy and the golden mushroom chaff of rotten composition, the plastics bag film that the bacterium bag is outer is sloughed, and spreads out and takes daylight drying in the sun method to make bacterium chaff drying, and dry back bacterium chaff water content is 10%-13%.Dried bacterium chaff adopts pulverizer to smash, and the powdery bacterium chaff maximum diameter after the processing is 0.3cm.
8.5 parts of edible fungus brans of meter by weight of crushed after being dried are mixed with 3.05 parts of auxiliary materials, the water that in mixing raw material, adds mixing raw material gross weight 90%, stir, adopting hydrochloric acid and the lime powder adjusting mixing raw material potential of hydrogen PH of 1M is 6.5, the mixing raw material that regulates is packed in the high temperature resistant eggplant-shape bottle into 121 ℃ of following moist heat sterilizations 20 minutes.Treat that the solid medium temperature reduces to 25 ℃-30 ℃, to be that 5% trichoderma harziarum spore suspension is inoculated on the mixing raw material then with the mixing raw material mass ratio, in relative humidity 70%, 25 ℃ of temperature, cultivate after 4 days under the no optical condition, do not have under the optical condition at 10 ℃ of low temperature and to cultivate 4 days culture materials and all be green, behind the mould spore of output wood.Fermentation ends, cultured material is air-dry with sterile air under 30-40 ℃, pulverizing makes particle diameter at 0.30-0.45mm, crosses 40 mesh sieves, promptly gets wooden mould spore microbial inoculum, and trichoderma harziarum spore type is mainly chlamydospore in the microbial inoculum, and quantity reaches 4.0-6.5 * 10 8Individual/the gram microbial inoculum.
Described golden mushroom chaff mixes with auxiliary material: adopt artificial turning method that flat mushroom bacterium chaff is counted by weight with auxiliary material and mix, 8.5 parts of bacterium chaffs, 1 part of sweet potato powder, 2 parts of dried silkworm chrysalis meals; 0.02 part of potassium primary phosphate, 0.02 part in sal epsom, 0.01 part of compound little vitamins B.Trichoderma harziarum spore suspension miospore quantity is 10 6Individual/ml.
Embodiment 3
Present embodiment adopts and consists of corn stalk powder 45%, chicken manure 15%, wheat bran 25%, urea 1%, cotton seed cake 2%, the culture medium waste Coprinus comatus bacterium chaff behind the production Coprinus comatus of lime powder 1% and lime carbonate 1%.Choose that mycelia is pure white, the material agllutination is real, do not have and go mouldy and the Coprinus comatus bacterium chaff of rotten composition, the plastics bag film that the bacterium bag is outer is sloughed, and spreads out and takes daylight drying in the sun method to make bacterium chaff drying, and dry back bacterium chaff water content is 15%-18%.Dried bacterium chaff adopts pulverizer to smash, and the powdery bacterium chaff maximum diameter after the processing is 0.5cm.
9 parts of edible fungus brans of meter by weight of crushed after being dried are mixed with 2.702 parts of auxiliary materials, the water that in mixing raw material, adds mixing raw material gross weight 85%, stir, adopting hydrochloric acid and the lime powder adjusting mixing raw material potential of hydrogen PH of 1M is 5.8, the mixing raw material that regulates is packed in the high temperature resistant eggplant-shape bottle into 115 ℃ of following moist heat sterilizations 40 minutes.Treat that the solid medium temperature reduces to room temperature, to be that 8% yellow green trichoderma (Trichoderma aureoviride) suspension is inoculated on the mixing raw material in relative humidity 80% then with the mixing raw material mass ratio, 30 ℃ of temperature, cultivate after 6 days under the no optical condition, do not have under the optical condition at 12 ℃ of low temperature and to cultivate 3 days culture materials and all be green, behind the mould spore of output wood.Fermentation ends, cultured material is air-dry with sterile air under 40-50 ℃, pulverizing makes particle diameter at 0.35-0.50mm, cross 40 mesh sieves, promptly get wooden mould spore microbial inoculum, yellow green trichoderma spore type major part is a chlamydospore in the microbial inoculum, small part is a conidium, and spore quantity reaches 1.2-1.6 * 10 8Individual/the gram microbial inoculum.
Described Coprinus comatus bacterium chaff mixes with auxiliary material: adopt artificial turning method that flat mushroom bacterium chaff is counted by weight with auxiliary material and mix, 9 parts of bacterium chaffs, 1.5 parts of Semen Maydis powder, 1.2 parts in wheat bran, 0.005 part of potassium primary phosphate, 0.005 part in sal epsom, 0.01 part of compound little vitamins B; Yellow green trichoderma spore suspension miospore quantity is 10 5Individual/ml.
Embodiment 4
Employing consists of the culture medium waste Pleurotus nebrodensis chaff behind the production Pleurotus nebrodensis of cotton seed hulls 80%, wood chip 10%, rice bran 8%, brown sugar 1%, gypsum 1%.Choose that mycelia is pure white, the material agllutination is real, do not have and go mouldy and the bar-shaped Pleurotus nebrodensis chaff of rotten composition, the plastics bag film that the bacterium bag is outer is sloughed, and spreads out and takes daylight drying in the sun method to make bacterium chaff drying, and dry back bacterium chaff water content is 14%.Dried bacterium chaff adopts pulverizer to smash, and the powdery bacterium chaff maximum diameter after the processing is 0.8cm.
8 parts of edible fungus brans of meter by weight of crushed after being dried are mixed with 3.03 parts of auxiliary materials, the water that in mixing raw material, adds mixing raw material gross weight 110%, stir, adopting hydrochloric acid and the lime powder adjusting mixing raw material potential of hydrogen PH of 1M is 6.2, the mixing raw material that regulates is packed in the high temperature resistant eggplant-shape bottle, 121 ℃ of following moist heat sterilizations 30 minutes, treat that the solid medium temperature reduces to room temperature, to be that 6% viride spore suspension is inoculated on the mixing raw material in relative humidity 75% then with the mixing raw material mass ratio, 26 ℃ of temperature, cultivate after 5 days under the no optical condition, do not have under the optical condition at 8 ℃ of low temperature and to cultivate 3 days culture materials and all be green, behind the mould spore of output wood.Fermentation ends, cultured material is air-dry with sterile air under 40-45 ℃, pulverizing makes particle diameter at 0.30-0.60mm, crosses 40 mesh sieves, promptly gets wooden mould spore microbial inoculum, and viride spore type is mainly chlamydospore in the microbial inoculum, and quantity reaches 0.8-1.4 * 10 8Individual/the gram microbial inoculum.
Described Pleurotus nebrodensis chaff mixes with auxiliary material: adopt artificial turning method that flat mushroom bacterium chaff is counted by weight with auxiliary material and mix, 8 parts of bacterium chaffs, 1.5 parts of sweet potato powder, 1.5 parts of dried silkworm chrysalis meals; 0.01 part of potassium primary phosphate, 0.01 part in sal epsom, 0.01 part of compound little vitamins B.Viride spore suspension miospore quantity is 10 8Individual/ml.
Embodiment 5
Difference from Example 1 is: described spore suspension is that viride (Trichodermaviride), trichoderma harziarum (Trichoderma harzianum), hook-shaped wood mould (Trichodermahamatum), yellow green trichoderma (Trichoderma aureoviride) and tangerine green trichoderma (Trichodermacitrinoriride) are pressed arbitrary proportion compound fungi strain, and suspension miospore quantity is 10 9Individual/ml.When described edible fungus bran mixes with auxiliary material, count 9 parts of edible fungus brans by weight, 2 parts of Semen Maydis powder, 1.5 parts in wheat bran, 0.025 part of potassium primary phosphate, 0.018 part in sal epsom, 0.02 part of compound little vitamins B; Or auxiliary material is 2 parts of sweet potato powder, 1 part of dried silkworm chrysalis meal; 0.005 part of potassium primary phosphate, 0.005 part in sal epsom, 0.005 part of compound little vitamins B.
Embodiment 6
Difference from Example 1 is: described spore suspension is that viride (Trichodermaviride), hook-shaped wood mould (Trichoderma hamatum) and tangerine green trichoderma (Trichodermacitrinoriride) are pressed arbitrary proportion compound fungi strain, and suspension miospore quantity is 10 7Individual/ml.When described edible fungus bran mixes with auxiliary material,, by weight count edible fungus bran 8, part, 1.25 parts of Semen Maydis powder, 1.4 parts in wheat bran, 0.08 part of potassium primary phosphate, 0.008 part in sal epsom, 0.006 part of compound little vitamins B; Or auxiliary material is 1.2 parts of sweet potato powder, 1.2 parts of dried silkworm chrysalis meals; 0.008 part of potassium primary phosphate, 0.006 part in sal epsom, 0.004 part of compound little vitamins B.
Embodiment 7
Difference from Example 1 is: described spore suspension is that hook-shaped wood mould (Trichodermahamatum) and tangerine green trichoderma (Trichoderma citrinoriride) are pressed arbitrary proportion compound fungi strain, and suspension miospore quantity is 10 6Individual/ml.When described edible fungus bran mixes with auxiliary material, count 8.5 parts of edible fungus brans by weight, 1.4 parts of Semen Maydis powder, 1.3 parts in wheat bran, 0.015 part of potassium primary phosphate, 0.012 part in sal epsom, 0.015 part of compound little vitamins B; Or auxiliary material is 1.6 parts of sweet potato powder, 1.4 parts of dried silkworm chrysalis meals; 0.015 part of potassium primary phosphate, 0.016 part in sal epsom, 0.018 part of compound little vitamins B.

Claims (10)

1. mould spore microbial inoculum of wood, it is characterized in that: the 8-9 of meter by weight part edible fungus bran of crushed after being dried is mixed with 2-4 part auxiliary material, regulating mixing raw material potential of hydrogen PH is 5.0-6.5, to be that the wooden mould spore suspension of 5%-10% is inoculated on the mixing raw material then with the mixing raw material mass ratio, at relative humidity 60-80%, temperature 25-30 ℃, cultivate after 4-6 days under the no optical condition, 8-12 ℃ of low temperature do not have under the optical condition cultivate treated the mould spore of output wood in 3-4 days after, with cultured dry materials, pulverize to be and produce wooden mould spore microbial inoculum;
Described auxiliary material is counted Semen Maydis powder 1-2 part by weight, wheat bran 1-1.5 part; Potassium primary phosphate 0.001-0.020 part, sal epsom 0.001-0.020 part, compound little vitamins B 0.001-0.020 part; Or sweet potato powder 1-2 part, dried silkworm chrysalis meal 1-2 part; Potassium primary phosphate 0.001-0.020 part, sal epsom 0.001-0.020 part, compound little vitamins B 0.001-0.020 part.
2. by the mould spore microbial inoculum of the described wood of claim 1, it is characterized in that: the wooden mould bacterial classification in the mould spore suspension of described wood is one or more in viride (Trichoderma viride), trichoderma harziarum (Trichoderma harzianum), hook-shaped wood mould (Trichoderma hamatum), yellow green trichoderma (Trichoderma aureoviride) and the tangerine green trichoderma (Trichoderma citrinoriride), presses arbitrary proportion compound fungi strain.
3. by the mould spore microbial inoculum of the described wood of claim 1, it is characterized in that: edible fungus bran is produced the substratum of edible mushrooms and is made up of major ingredient and auxiliary material, by weight the content meter for producing the substratum behind the edible mushrooms according to a conventional method, wherein major ingredient accounts for 92-98%, and auxiliary material accounts for 2-8%; Major ingredient is by cotton seed hulls, wood sawdust, straw, corn cob, bagasse, corn stalk powder, soya bean stem meal, wheat bran, rice chaff, rice husk, cotton straw powder, wheat straw, weeds, and bagasse, vinasse, vinegar are poor, 2~4 kinds of mixtures in xylose residue, rapeseed cake, pig manure, chicken manure, horsehit, cow dung, human excrement, pigsty fertilizer and the pond sludge soil are formed; Auxiliary material is made up of the 3-6 kind mixture in soybean cake powder, cotton seed cake, wheat bran, urea, composite fertilizer, calcium superphosphate, fused(calcium magnesium)phosphate, lime powder, terra alba, lime carbonate, plant ash, potassium primary phosphate, sucrose, brown sugar, ammoniacal liquor, dried silkworm chrysalis meal, glucose and the VITMAIN B1; The edible fungus bran mycelia is pure white, the material agllutination is real, nothing is gone mouldy and rotten composition the C of bacterium chaff: N=20-35: 1, and C: P=120-270: 1, the bacterium chaff water content 10-25% of drying, the bacterium chaff particle diameter 0.2-1.0cm of pulverizing.
4. by the mould spore microbial inoculum of the described wood of claim 1, it is characterized in that: the water that adds mixing raw material weight 70%-120% in the described mixing raw material; The potential of hydrogen of described mixing raw material adopts hydrochloric acid and lime powder to regulate.
5. by the mould spore microbial inoculum of the described wood of claim 1, it is characterized in that: the wooden mould spore suspension miospore quantity of described inoculation is 10 5-10 9Individual/ml; Described mixing raw material is packed in high-temperature resistance plastice bag or the fermenting container, 115 ℃-121 ℃ of following moist heat sterilization 20-60 minutes.
6. according to the preparation method of the wooden mould spore microbial inoculum of claim 1, it is characterized in that: the 8-9 of meter by weight part edible fungus bran of crushed after being dried is mixed with 2-4 part auxiliary material, regulating mixing raw material potential of hydrogen PH is 5.0-6.5, to be that the wooden mould spore suspension of 5%-10% is inoculated on the mixing raw material at relative humidity 60-80% then with the mixing raw material mass ratio, temperature 25-30 ℃, cultivate after 4-6 days under the no optical condition, 8-12 ℃ of low temperature do not have under the optical condition cultivate treated the mould spore of output wood in 3-4 days after, with cultured dry materials, pulverizing is produces wooden mould spore microbial inoculum;
Described auxiliary material is counted Semen Maydis powder 1-2 part by weight, wheat bran 1-1.5 part; Potassium primary phosphate 0.001-0.020 part, sal epsom 0.001-0.020 part, compound little vitamins B 0.001-0.020 part; Or sweet potato powder 1-2 part, dried silkworm chrysalis meal 1-2 part; Potassium primary phosphate 0.001-0.020 part, sal epsom 0.001-0.020 part, compound little vitamins B 0.001-0.020 part.
7. press the preparation method of the mould spore microbial inoculum of the described wood of claim 6, it is characterized in that: the wooden mould bacterial classification in the mould spore suspension of described wood is one or more in viride (Trichoderma viride), trichoderma harziarum (Trichoderma harzianum), hook-shaped wood mould (Trichoderma hamatum), yellow green trichoderma (Trichoderma aureoviride) and the tangerine green trichoderma (Trichoderma citrinoriride), presses arbitrary proportion compound fungi strain.
8. press the preparation method of the mould spore microbial inoculum of the described wood of claim 6, it is characterized in that: edible fungus bran is produced the substratum of edible mushrooms and is made up of major ingredient and auxiliary material, by weight the content meter for producing the substratum behind the edible mushrooms according to a conventional method, wherein major ingredient accounts for 92-98%, and auxiliary material accounts for 2-8%; Major ingredient is by cotton seed hulls, wood sawdust, straw, corn cob, bagasse, corn stalk powder, soya bean stem meal, wheat bran, rice chaff, rice husk, cotton straw powder, wheat straw, weeds, and bagasse, vinasse, vinegar are poor, 2~4 kinds of mixtures in xylose residue, rapeseed cake, pig manure, chicken manure, horsehit, cow dung, human excrement, pigsty fertilizer and the pond sludge soil are formed; Auxiliary material is made up of the 3-6 kind mixture in soybean cake powder, cotton seed cake, wheat bran, urea, composite fertilizer, calcium superphosphate, fused(calcium magnesium)phosphate, lime powder, terra alba, lime carbonate, plant ash, potassium primary phosphate, sucrose, brown sugar, ammoniacal liquor, dried silkworm chrysalis meal, glucose and the VITMAIN B1; The edible fungus bran mycelia is pure white, the material agllutination is real, nothing is gone mouldy and rotten composition the C of bacterium chaff: N=20-35: 1, and C: P=120-270: 1, the bacterium chaff water content 10-25% of drying, the bacterium chaff particle diameter 0.2-1.0cm of pulverizing.
9. by the preparation method of the mould spore microbial inoculum of the described wood of claim 6, it is characterized in that: the water that adds mixing raw material weight 70%-120% in the described mixing raw material; The potential of hydrogen of described mixing raw material adopts hydrochloric acid or lime powder to advance to regulate.
10. by the preparation method of the mould spore microbial inoculum of the described wood of claim 6, it is characterized in that: the wooden mould spore suspension miospore quantity of described inoculation is 10 5-10 9Individual/ml; Described mixing raw material is packed in high-temperature resistance plastice bag or the fermenting container, 115 ℃-121 ℃ of following moist heat sterilization 20-60 minutes.
CN2008100111865A 2008-04-25 2008-04-25 Trichodrema spp. microbial agent and preparation method thereof Expired - Fee Related CN101565688B (en)

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CN101870596B (en) * 2010-06-12 2013-01-16 暨南大学 Method of preparing preparation for promoting pollination and fruit setting of xeromorphic crop by Trichodermaharzianum solid fermentation
CN102080052B (en) * 2010-12-17 2012-07-04 中国科学院南京土壤研究所 Trichoderma strain FS10-C for promoting contaminated soil phytoremediation and preparation method of microbial agent of trichoderma strain
CN102351593A (en) * 2011-06-17 2012-02-15 天津市园艺工程研究所 Method for preparing microbial biocontrol organic fertilizer from sludge and chaff of edible fungi
CN104211630B (en) * 2013-05-30 2016-05-04 福州大学 A kind of alkaloid compound and application thereof that comes from tangerine green trichoderma
CN103555648B (en) * 2013-08-27 2015-06-17 云南省烟草公司临沧市公司 Method for culturing Trichoderma chlamydospore by using Caenorhabditis elegans, and application of chlamydospore
CN103992956A (en) * 2014-05-23 2014-08-20 山东省科学院中日友好生物技术研究中心 Method for preparing trichoderma biocontrol microbial agent by using edible fungus chaff
CN104400001B (en) * 2014-11-14 2016-03-16 东北林业大学 A kind of method utilizing the mould extracellular fluid of hook-shaped wood to prepare Ag/AgCl nano composite material
CN106213069B (en) * 2016-07-15 2020-11-13 上海师范大学 Artificial feed for larva of onychomycosis
WO2018049685A1 (en) * 2016-09-19 2018-03-22 王亮 Cultivation substrate and cultivation method for flammulina velutipes
CN108401794A (en) * 2018-02-12 2018-08-17 重庆市中药研究院 A kind of armillaria mellea accreting with Rhizoma Gastrodiae liquid spawn production method and cultigen special culture media
CN109628323B (en) * 2018-12-28 2019-09-27 云南星耀生物制品有限公司 Trichoderma Viride method and its water dispersible granules

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