CN106613312A - Method for harvesting selenium-rich ganoderma-lucidum-karst spore powder - Google Patents
Method for harvesting selenium-rich ganoderma-lucidum-karst spore powder Download PDFInfo
- Publication number
- CN106613312A CN106613312A CN201610869593.4A CN201610869593A CN106613312A CN 106613312 A CN106613312 A CN 106613312A CN 201610869593 A CN201610869593 A CN 201610869593A CN 106613312 A CN106613312 A CN 106613312A
- Authority
- CN
- China
- Prior art keywords
- ganoderma
- sesame
- frame
- lucidum
- karst
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01D—HARVESTING; MOWING
- A01D91/00—Methods for harvesting agricultural products
- A01D91/04—Products growing above the soil
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a method for harvesting selenium-rich ganoderma-lucidum-karst spore powder. The method includes the following technical steps that 1) a ganoderma-lucidum-karst emerging frame is made; 2) ganoderma-lucidum-karst fungus bags are directly put on a layer frame; 3) the fungus bags are subjected to ganoderma-lucidum-karst emerging, then a selenium-rich nutrient solution is sprayed till the surface of ganoderma lucidum karst is evenly covered, the temperature of a ganoderma-lucidum-karst emerging room is controlled to be 26 DEG C, the humidity is 85% to 90%, when the ganoderma lucidum karst grows in a cover-opening mode and sprays the spore powder, a surface plate of 300 cm*100 cm is fixed at the top of the ganoderma-lucidum-karst emerging frame, the whole ganoderma-lucidum-karst emerging frame is covered with a agricultural film with the thickness of 0.006 cm, the lower portion of the film is connected with the concave surface of a PVC plate through scotch tapes, and after the ganoderma lucidum karst sprays the spore powder, the temperature of the ganoderma-lucidum-karst emerging room is controlled to be 25 DEG C, and the humidity is 70% to 75% till the spore powder is completely harvested.
Description
Technical field
The present invention relates to technical field of edible fungi production, more particularly to the collecting method of Se-rich lucid ganoderma conidia powder.
Background technology
Glossy ganoderma is the traditional mushroom treasure of China, enjoys " celestial grass " good reputation.Glossy ganoderma (scientific name：Ganoderma Lucidum
Karst), also known as polyporus lucidus, celestial grass, another name red sesame, red sesame, Ganoderma Lucidum, Ganoderma, 10000 years gill fungus, polyporus lucidus are that Polyporaceae is planted
The complete stool of thing red sesame or purple sesame, is the fructification of On Polyporaceae glossy ganoderma.Profile is in umbrella, cap kidney shape, semicircle or near
It is circular.《Sheng Nong's herbal classic》With《Compendium of Materia Medica》The drug effect and function of glossy ganoderma are described in detail.Glossy ganoderma is formal as medicine
Recorded by NF, be the new resource food of state approval, can medicine-food two-purpose.Glossy ganoderma is used as possessing thousands of years medicinal history
Chinese tradition valuable ingredient of traditional Chinese medicine, with invigorating qi for tranquilization, relieving cough and asthma effect, for dizziness egersis, palpitation, consumptive disease cough and asthma.
Glossy ganoderma possesses very high medical value, confirms that glossy ganoderma is for enhancing people through the modern pharmacology research of scientific research institution's many decades
Body immunity, adjusts blood sugar, controls blood pressure, and adjuvant therapy chemicotherapy, liver protecting promotes the aspects such as sleep to be respectively provided with significantly treatment
Effect.GL-B is one of principle active component of glossy ganoderma medicinal function, is primarily present in glossy ganoderma cell wall inwall, and major part is
Beta glucan, its monose is constituted in addition to containing a large amount of glucose, also containing a small amount of arabinose, wood sugar, fucose, rhamnose,
Galactolipin and mannose etc., they are mostly bonded with glucosides such as (1-3), (1-4) and (1-6), with extensive biologically active,
Such as strengthen immunity, removing human free radical, protection angiocarpy, radioresistance, protection liver, antitumor effect.Triterpenes
It is one of active ingredient of glossy ganoderma, triterpenes is no less than 100 kinds of contained by glossy ganoderma, wherein based on tetracyclic triterpenes, the bitter taste of glossy ganoderma
It is relevant with contained triterpenes, there is CDCC to human hepatoma cell, can also suppress the release of histamine, with liver protection and
Antiallergy etc. is acted on.
Selenium is trace element needed by human.Selenium participates in various selenoenzymes and Selenonic protein in synthesized human.Wherein paddy Guang
Sweet peptide peroxidase, is catalyzed hydroperoxides or MDA is changed into water or various alcohols in vivo, eliminates
Free radical protects biomembrane from oxidative damage to biomembranous attack；Selenium participates in constituting the de- iodine enzyme of iodate thyroid gland amino acid.
In vivo selenium and vitamin E collaboration, can protect cell membrane, prevent the oxidation of unrighted acid.Trace selenium has protective effect on cancer risk
And liver-protective effect.Selenium can improve human immunity, promote the propagation of lymphocyte and the synthesis of antibody and immunoglobulin (Ig).
Selenium has the effect for significantly suppressing and protecting to kinds cancers such as colon cancer, cutaneum carcinoma, liver cancer, breast cancer, and it is in body
Mesostate methyl enol have stronger active anticancer.The battalion such as selenium and vitamin E, allicin, linoleic acid, germanium, zinc
Support element and there is the oxidation resistant effect of collaboration, increase antioxidation activity.Meanwhile, selenium has the work for mitigating and alleviating heavy metal toxicity
With.
Lucidum spore powder is that glossy ganoderma growth and maturity phase ejects ultrafine spore from cap, is that the reproduction of glossy ganoderma is thin
Born of the same parents.Substantial amounts of experiment confirms that conidia powder has functions that stronger antitumor, immunological regulation, regulation blood-fat and blood sugar and to nerve
System, angiocarpy and respiratory system have regulation improvement result and are pursued by society.Due to lucidum spore powder it is extremely thin
It is little, can all over the sky dance in the air with air flow, therefore collection conidia powder is just particularly important.In long-term practice, people
The collection method of several main lucidum spore powders has been invented according to local cultivation custom and practical experience, it is specific as follows：1、
Ground embrane method：The every row glossy ganoderma medial exhaust double-deck strip mulch film after cultivation of glossy ganoderma, receives the conidia powder of landing.In harvesting glossy ganoderma
During entity, cap surface spore is painted in bucket with banister brush, then harvest conidia powder, mulch film powder is only harvested during harvesting, under
Layer conidia powder is given it up.The advantage of the method is that glossy ganoderma entirety growing environment is easy to operate, low cost；Have the disadvantage the spore collected
Some sandy soil can be contained in sub- powder, the purity of spore is not high enough.2nd, sleeve adopts powder method：When glossy ganoderma will be ripe, in Ganoderma Lucidum
Handle base portion puts the ultra-thin vinyl bag of 0.02mm, and bag bottom tightens, and sack upward, by the size of glossy ganoderma cardboard cylinder is covered in bag,
High 15cm, diameter 3-15cm, cover a cardboard on nozzle, prevent conidia powder from escaping.Bagging adopts powder it is noted that ventilation, prevents mould
Become, adopt powder phase film two and scatter all the time.The advantage of the method is the conidia powder very high purity collected, and has the disadvantage high cost.3、
Blower fan absorption method：The proportion of Reishi sporule is lighter, is collected with blower fan, and 200-300 square metre of sesame canopy is received using two spores
Storage.When Reishi sporule starts release, two spore collectors facing away from and be placed in the middle of sesame canopy, away from ground 1-1.5
Meter Gao, when typically in fine day morning 4-8,5-8 in afternoon and cloudy whole day turns on the power collection lucidum spore powder.The method
Advantage is operation facility, has the disadvantage that in the conidia powder collected some dusts can be mixed with.
The content of the invention
The purpose of the present invention is to overcome the deficiencies in the prior art, there is provided the collecting method of Se-rich lucid ganoderma conidia powder.
The collecting method of Se-rich lucid ganoderma conidia powder of the present invention, is achieved by the following technical solution：
1) making of sesame frame is gone out：Go out a height of 300cm × 50cm × 180cm of sesame frame length and width, each goes out sesame frame and is constituted by 4 layers,
Go out sesame frame bottom with ground at a distance of 20cm, the spacing of remainder layer is 40cm；
Dismountable 300cm × 100cm surface plate is installed at each top for going out sesame frame, and each bottom for going out sesame frame is used
The PVC board of food-grade makes the groove of matrix, and in the centre of groove the circular hole of a diameter 10cm is reserved, and respectively goes out and reserve between sesame frame 70cm
Passage, each reserved 50cm passages in both sides；
2) glossy ganoderma bacterium bag is directly placed in layer frame, towards both sides, per layer is put 4 to 5 layers of glossy ganoderma to glossy ganoderma bacterium bag inoculation mouth
Bacterium bag；
3) bacterium bag goes out after sesame, sprays Selenium-rich nutrient solution, is defined by uniform fold glossy ganoderma surface, controls out sesame room temperature 26
DEG C, humidity 85%-90%, it is ensured that sufficient ventilation promotes growing for glossy ganoderma treats that glossy ganoderma is uncapped growth, will spray spore
During powder, the surface plate of 300cm × 100cm is fixed on into out sesame top of the trellis, is gone out entirely with the agricultural film that thickness is 0.006cm
Sesame frame is covered, film bottom and the transparent gemel connection of PVC board concave surface, after glossy ganoderma injection conidia powder, is controlled glossy ganoderma and is gone out sesame room temperature
Degree 25 DEG C, humidity 70%-75%, until conidia powder harvesting is finished；
Selenium-rich nutrient solution of the present invention, is prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, 1.0 parts of L- selenomethionines, 5 parts of corn flour；
2. after the raw material by step 1. mixes, steam blasting tank is put into, pressure 4MPa is heated to 150 DEG C, keeps 2min,
Then quick release pressure obtains slurries in 1-3s, is the ripe withered grass bud of 10% access culture by percent by volume at 25 DEG C
Spore bacillus liquid spawn, in being put into fermentation tank, is passed through filtrated air in fermentation tank, be stirred fermentation, speed of agitator 250r/
Min, fermentation time is 4h, and temperature is kept for 35-37 DEG C, after fermentation ends, rapidly temperature is risen to into 70 DEG C and keeps 1.0h inactivations, is obtained
Arrive.
The cultural method of the bacillus subtilis liquid spawn is：Using triangular flask strain cultivation, below attach most importance to
Amount percentage, by albumen slurry 30.0-35.0% for processing, KH2P041.0%, MgS042.0%, NaCl0.5% are put into three
In the bottle of angle, stir, adjust pH to 5.6,125 DEG C of sterilizing 10min are cooled to 25 DEG C, access the withered grass through inclined-plane culture
Bacillus, 28 DEG C of shaking table cultures 60-100h, for fermentation tank inoculation.
Step 1 of the present invention) described in the centre in groove reserve the circular hole of a diameter 10cm, be conidia powder for convenience
Collect；The each reserved 50cm passages in described both sides, are easy for the harvesting of glossy ganoderma.
Step 3) described in conidia powder harvesting, be fresh, the not mildew for ensureing spore, a spore is collected preferably per 15 days
Powder.
Compared with prior art, the invention has the advantages that：
1st, Selenium-rich nutrient solution of the prior art, usually using inorganic selenium, particularly sodium selenite and sodium selenate, sub- selenium
Sour potassium and potassium selenate etc., obtain from the byproduct of metal mineral reserves, and inorganic selenium has larger toxicity, and are difficult to be absorbed, as
Nutrient solution, assimilation effect is bad.The selenomethionine that the present invention is used, is a kind of organic selenium source, compared with inorganic selenium, selenomethionine
With safe and efficient advantage, it can substitute some methionine and be attached to one reversible selenium depots of formation on body protein,
And there is no toxic hazard.
2nd, selenomethionine is that selenium is present in a kind of seleno amino in plant and feed grain with organic form in nature
The plant growths such as acid, cereal and forage crop are not necessarily required to selenomethionine, but with methionine synthesis they can utilize ring
The selenite and selenate synthesis selenomethionine being particularly in border in soil.It is more using selenium in soil, in plant
Selenomethionine content is higher.At present, selenomethionine is mainly derived from natural selenium yeast, is that Organic Selenium is primarily present in selenium yeast
Form, but selenomethionine has dense yeast taste, directly water-soluble rear sprinkling, and absorption efficiency is low and influences whether finally to produce
The taste and flavor of product.Bacillus subtillis, is one kind of Bacillus, and bacillus subtilis thalline itself synthesizes alphalise starch
The enzymes such as enzyme, protease, lipase, cellulase, equivalent to complex enzyme, the present invention is by selenomethionine mixing Ko subtilis bar
Bacterium after fermentation, the zymasis carried using selenomethionine product is fermented, and on the one hand enters glossy ganoderma beneficial to selenium element, on the other hand
With methionine synthesis they can utilize in environment selenite and selenate synthesis selenomethionine in particularly soil, also
Can be effectively improved, dissolve yeast flavor.
3rd, steam blasting be using high-temperature high-pressure steam process biomass material, and realized by moment pressure leak process it is right
The surface texture of raw material changes, Component seperation and hemicellulose degradation, is generally used for maize straw, straw and Wooden Biomass etc.
The pretreatment of raw material.The present invention is by " being put into steam blasting tank, pressure 4MPa is heated to 150 DEG C, 2min is kept, then in 1-
Quick release pressure in 3s ", 0 is reduced to by moment by pressure, on the one hand can change to sterilize by the moment of pressure, another
Aspect can effectively be refined, disperse selenomethionine, advantageously in the absorption of glossy ganoderma.
4th, prior art is typically all to wait the ejection of ripe Reishi sporule under field conditions (factors), the peak of spore ejection
During usually fine day morning 4-8,5-8 in afternoon when and cloudy whole day.The present invention is located by introducing heat stress, control culturing room
The temperature in room, humidity, on the one hand promote the ejection amount of ripe Reishi sporule by thermal stimulus, and on the other hand, temperature is improved,
The acquisition time of the spore ejection of ripe glossy ganoderma, and then the acquisition time of brachysporous ejection of contracting can be shortened.
5th, the loss of lucidum spore powder of the present invention is little, purity height (almost up to 100%), and quality is full, compared with bagging,
Operating efficiency is significantly improved, and sheaf space in energy effectively utilizes.
Specific embodiment
Below with embodiment, the invention will be further described, but the invention is not limited in these embodiments.
Embodiment 1-3：
In order to verify the technique effect of the present invention, 2016, the product of 3 batches is produced using the technology of the present invention, each
The method of batch is as follows:
1) making of sesame frame is gone out：Go out a height of 300cm × 50cm × 180cm of sesame frame length and width, each goes out sesame frame and is constituted by 4 layers,
Go out sesame frame bottom with ground at a distance of 20cm, the spacing of remainder layer is 40cm；
Dismountable 300cm × 100cm surface plate is installed at each top for going out sesame frame, and each bottom for going out sesame frame is used
The PVC board of food-grade makes the groove of matrix, and in the centre of groove the circular hole of a diameter 10cm is reserved, and respectively goes out and reserve between sesame frame 70cm
Passage, each reserved 50cm passages in both sides；
2) glossy ganoderma bacterium bag is directly placed in layer frame, towards both sides, per layer is put 4 to 5 layers of glossy ganoderma to glossy ganoderma bacterium bag inoculation mouth
Bacterium bag；
3) bacterium bag goes out after sesame, sprays Selenium-rich nutrient solution, is defined by uniform fold glossy ganoderma surface, controls out sesame room temperature 26
DEG C, humidity 85%-90%, it is ensured that sufficient ventilation promotes growing for glossy ganoderma treats that glossy ganoderma is uncapped growth, will spray spore
During powder, the surface plate of 300cm × 100cm is fixed on into out sesame top of the trellis, is gone out entirely with the agricultural film that thickness is 0.006cm
Sesame frame is covered, film bottom and the transparent gemel connection of PVC board concave surface, after glossy ganoderma injection conidia powder, is controlled glossy ganoderma and is gone out sesame room temperature
Degree 25 DEG C, humidity 70%-75%, a conidia powder was collected per 15 days, until conidia powder harvesting is finished；
Described Selenium-rich nutrient solution, is prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, 1.0 parts of L- selenomethionines, 5 parts of corn flour；
2. after the raw material by step 1. mixes, steam blasting tank is put into, pressure 4MPa is heated to 150 DEG C, keeps 2min,
Then quick release pressure obtains slurries in 1-3s, is the ripe withered grass bud of 10% access culture by percent by volume at 25 DEG C
Spore bacillus liquid spawn, in being put into fermentation tank, is passed through filtrated air in fermentation tank, be stirred fermentation, speed of agitator 250r/
Min, fermentation time is 4h, and temperature is kept for 35-37 DEG C, after fermentation ends, rapidly temperature is risen to into 70 DEG C and keeps 1.0h inactivations, is obtained
Arrive.
The cultural method of the bacillus subtilis liquid spawn is：Using triangular flask strain cultivation, below attach most importance to
Amount percentage, by albumen slurry 30.0-35.0% for processing, KH2P041.0%, MgS042.0%, NaCl0.5% are put into three
In the bottle of angle, stir, adjust pH to 5.6,125 DEG C of sterilizing 10min are cooled to 25 DEG C, access the withered grass through inclined-plane culture
Bacillus, 28 DEG C of shaking table cultures 60-100h, for fermentation tank inoculation.
Comparative example 1：
1) making of sesame frame is gone out：Go out a height of 300cm × 50cm × 180cm of sesame frame length and width, each goes out sesame frame and is constituted by 4 layers,
Go out sesame frame bottom with ground at a distance of 20cm, the spacing of remainder layer is 40cm；
Dismountable 300cm × 100cm surface plate is installed at each top for going out sesame frame, and each bottom for going out sesame frame is used
The PVC board of food-grade makes the groove of matrix, and in the centre of groove the circular hole of a diameter 10cm is reserved, and respectively goes out and reserve between sesame frame 70cm
Passage, each reserved 50cm passages in both sides；
2) glossy ganoderma bacterium bag is directly placed in layer frame, towards both sides, per layer is put 4 to 5 layers of glossy ganoderma to glossy ganoderma bacterium bag inoculation mouth
Bacterium bag；
3) bacterium bag goes out after sesame, it is ensured that sufficient ventilation promotes growing for glossy ganoderma, treats that glossy ganoderma is uncapped growth, will be sprayed
During conidia powder, the surface plate of 300cm × 100cm is fixed on into out sesame top of the trellis, will be whole with the agricultural film that thickness is 0.006cm
It is individual go out sesame frame cover, film bottom and the transparent gemel connection of PVC board concave surface, after glossy ganoderma injection conidia powder, the collection one per 15 days
Secondary conidia powder, until conidia powder harvesting is finished.
Comparative example 2：
Method changes L- selenomethionines in Selenium-rich nutrient solution into sodium selenite with embodiment 1-3.
Comparative example 3：
Bagging collects lucidum spore powder.
Comparative result：
As a result show, the receipts powder time can be shortened by the technology of the present invention, increased and received powder amount.Can be with from specific embodiment
Find out, receive the powder time and compare control group, shorten 20-23 days or so, receiving powder amount increases by more than 50%, obtains Se content in conidia powder
Compare comparative example and improve more than 300-400%.
Claims (3)
1. the collecting method of Se-rich lucid ganoderma conidia powder, it is characterised in that including following technical step：
1) making of sesame frame is gone out：Go out a height of 300cm × 50cm × 180cm of sesame frame length and width, each goes out sesame frame and is constituted by 4 layers, goes out sesame
With ground at a distance of 20cm, the spacing of remainder layer is 40cm to frame bottom；
Dismountable 300cm × 100cm surface plate is installed at each top for going out sesame frame, and each goes out the bottom food of sesame frame
The PVC board of level makes the groove of matrix, and in the centre of groove the circular hole of a diameter 10cm is reserved, and respectively to go out reserve between sesame frame 70cm and leads to
Road, each reserved 50cm passages in both sides；
2) glossy ganoderma bacterium bag is directly placed in layer frame, towards both sides, per layer is put 4 to 5 layers of glossy ganoderma bacterium bag to glossy ganoderma bacterium bag inoculation mouth；
3) bacterium bag goes out after sesame, sprays Selenium-rich nutrient solution, is defined by uniform fold glossy ganoderma surface, controls out 26 DEG C of sesame room temperature, wet
Degree 85%-90%, it is ensured that sufficient ventilation promotes growing for glossy ganoderma, treats that glossy ganoderma is uncapped growth, when will spray conidia powder,
The surface plate of 300cm × 100cm is fixed on into out sesame top of the trellis, sesame frame will be entirely gone out with the agricultural film that thickness is 0.006cm
Cover, film bottom and the transparent gemel connection of PVC board concave surface, after glossy ganoderma injection conidia powder, control glossy ganoderma and go out sesame room temperature 25
DEG C, humidity 70%-75%, until conidia powder harvesting finish；
Described Selenium-rich nutrient solution, is prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, 1.0 parts of L- selenomethionines, 5 parts of corn flour；
2. after the raw material by step 1. mixes, steam blasting tank is put into, pressure 4MPa is heated to 150 DEG C, keeps 2min, then
Quick release pressure obtains slurries in 1-3s, is the ripe bacillus subtilis of 10% access culture by percent by volume at 25 DEG C
Bacteria liquid bacterial classification, in being put into fermentation tank, is passed through filtrated air in fermentation tank, be stirred fermentation, and speed of agitator 250r/min sends out
The ferment time is 4h, and temperature is kept for 35-37 DEG C, after fermentation ends, rapidly temperature is risen to into 70 DEG C and keeps 1.0h inactivations, is obtained.
2. the collecting method of Se-rich lucid ganoderma conidia powder according to claim 1, it is characterised in that：The bacillus subtilis
The cultural method of liquid spawn is：It is below percetage by weight using triangular flask strain cultivation, by the albumen powder for processing
Slurry 30.0-35.0%, KH2P041.0%, MgS042.0%, NaCl0.5% are put in triangular flask, are stirred, adjust pH to
5.6,125 DEG C of sterilizing 10min, are cooled to 25 DEG C, access the Bacillus subtilis strain through inclined-plane culture, 28 DEG C of shaking table cultures
60-100h, for fermentation tank inoculation.
3. the collecting method of Se-rich lucid ganoderma conidia powder according to claim 1, it is characterised in that：Step 3) described in spore
Powder is harvested, and a conidia powder was collected per 15 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610869593.4A CN106613312A (en) | 2016-09-30 | 2016-09-30 | Method for harvesting selenium-rich ganoderma-lucidum-karst spore powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610869593.4A CN106613312A (en) | 2016-09-30 | 2016-09-30 | Method for harvesting selenium-rich ganoderma-lucidum-karst spore powder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106613312A true CN106613312A (en) | 2017-05-10 |
Family
ID=58854050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610869593.4A Pending CN106613312A (en) | 2016-09-30 | 2016-09-30 | Method for harvesting selenium-rich ganoderma-lucidum-karst spore powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106613312A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934752A (en) * | 2018-06-28 | 2018-12-07 | 靖西市秀美边城农业科技有限公司 | A kind of method of substituting stuff cultivation giant glossy ganoderma |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040071089A (en) * | 2004-07-06 | 2004-08-11 | 김현채 | Cultivation of mulberry tree containing selenium and germanium |
| CN102318504A (en) * | 2011-08-08 | 2012-01-18 | 四川农业大学 | Method for collecting spore powder by cultivating ganoderma with layered rack type stereoscopic bag material |
| CN102719505A (en) * | 2012-05-14 | 2012-10-10 | 梁金钟 | Method for preparing vegetable protein small peptide by microoprganism fermentation |
| CN103145488A (en) * | 2012-11-28 | 2013-06-12 | 梁树森 | Multiple microbial manure applied in summer to increase quality of black tea and preparation method thereof |
-
2016
- 2016-09-30 CN CN201610869593.4A patent/CN106613312A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040071089A (en) * | 2004-07-06 | 2004-08-11 | 김현채 | Cultivation of mulberry tree containing selenium and germanium |
| CN102318504A (en) * | 2011-08-08 | 2012-01-18 | 四川农业大学 | Method for collecting spore powder by cultivating ganoderma with layered rack type stereoscopic bag material |
| CN102719505A (en) * | 2012-05-14 | 2012-10-10 | 梁金钟 | Method for preparing vegetable protein small peptide by microoprganism fermentation |
| CN103145488A (en) * | 2012-11-28 | 2013-06-12 | 梁树森 | Multiple microbial manure applied in summer to increase quality of black tea and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934752A (en) * | 2018-06-28 | 2018-12-07 | 靖西市秀美边城农业科技有限公司 | A kind of method of substituting stuff cultivation giant glossy ganoderma |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103145462B (en) | Enoki mushroom stock culture material and making method thereof | |
| CN101623098B (en) | Health care food containing fermentation cordyceps and lucid ganoderma and preparation method thereof | |
| CN101743854B (en) | Method for preparing morchella mycelium by using dendrobium fiber as substrate | |
| CN102027857A (en) | New method for commercial field cultivation of toadstool | |
| CN102612985A (en) | Production technology for cordyceps militaris mycelium | |
| CN102835663B (en) | Chinese caterpillar fungus mycelium powder production process | |
| CN102550300A (en) | Method for cultivating cordyceps sinensis by taking unhusked rice as culture medium | |
| CN103262753A (en) | Auricularia polytricha soil-covered cultivation technology | |
| CN104488565A (en) | Method for cultivating Tibetan lucidum | |
| CN104311329A (en) | Culture medium capable of increasing nutrient content of agrocybe cylindracea | |
| CN102924171A (en) | Earthing material and method for cultivating clitocybe maximas | |
| CN101897270A (en) | Production technology of Cordyceps sinensis mycelium | |
| CN103518528A (en) | Floating type antrodia camphorata cultivation method capable of increasing triterpenes content | |
| CN110214626A (en) | A kind of straw mushroom cultural method | |
| CN102925527A (en) | Method for mixing and fermenting flammulina velutipes and lucid ganoderma | |
| CN106431571A (en) | Ganoderma lucidum karst cultivation method for improving ganoderma lucidum polysaccharides and selenium content | |
| CN108718915A (en) | Improve the culture medium and cultural method of pleurotus edible fungus yield | |
| CN104641944A (en) | Method for culturing cordyceps sinensis by taking unhulled wheat as culture medium | |
| CN106631344A (en) | Lucid ganoderma bag cultivation culture medium capable of increasing content of ganoderan and selenium | |
| CN106852256A (en) | A kind of composite cultivation method of black fungus | |
| KR20030026140A (en) | Mycellium production method of Cordyceps militaris, Inonotus obliquus and Phellinus linteus mushroom by use germinated grains and edible filber | |
| CN108383652A (en) | Agricultural wastes cultivate rich selenium germanium green organic element nutrition fertilizer | |
| CN112514734A (en) | Indoor cultivation method for phellinus igniarius | |
| CN107177515A (en) | A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation | |
| CN106613312A (en) | Method for harvesting selenium-rich ganoderma-lucidum-karst spore powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170510 |
|
| RJ01 | Rejection of invention patent application after publication |