The anti-ageing compositionss of whitening and cosmetics
Technical field
The present invention relates to cosmetic technical field, particularly to the anti-ageing compositionss of whitening and cosmetics.
Background technology
Nowadays problem of environmental pollution is serious, and the operating pressure of people is big, and life is irregular, leads to skin to occur intensely dark
The various problems such as lax, microgroove mottle, drying.Everybody has a love of beauty, and people save oneself by various cosmetics
Skin.Most of cosmetics contain the materials such as lead, hydrargyrum, benzene, preservative, hormone although can temporarily make the skin be in the market
Existing glossy soft state, but life-time service that horny layer can be made to come off is thinning, lead to red swelling of the skin, form mottle, effect fits to obtain it
Instead, the safety of therefore cosmetics becomes the primary factor considering during consumer's purchase cosmetics.Flying with cosmetic industry
Speed development, the cry of " component naturalization " and " going back to nature " has become the trend of cosmetics industry, the raw material sky of cosmetics
Rightization is extremely paid attention to.Pure natural whitening composition is increasingly becoming one of cosmetics key component.
Melanic content is the important factor in order of the skin color depth, and melanic content is more, the colour of skin of skin
More black.Melanin is by tyrosine under the catalysis of tryrosinase, through generating a series of metabolic response shapes such as DOPA, DOPA quinone
Become, therefore, in place of suppression tyrosinase activity is the key reducing melanin generation.In addition, leading to the modal of aging
Endogenouss Some Circulating Factors are free radicals, thus particularly important for defying age, removing free radical.In recent years, tryrosinase suppression
Agent, antioxidant research very active, conventional has salicylic acid, Azelaic Acid class, arbutin, fruit acid, ascorbic acid, kojic acid
Or derivant being synthesized by them etc., the part but these all come with some shortcomings, the such as cytotoxicity of hydroquinone and kojic acid, skin
Skin zest is larger;Ascorbic acid is unstable, easily oxidized, and percutaneous absorbtion is poor;Fruit acid has certain zest to skin, long
Phase leads to skin more sensitive to ultraviolet using meeting.Therefore, find natural cheese ammonia stable, effective and that human body is had no adverse reaction
Sour enzyme inhibitor, powerful antioxidant have become the trend of cosmetic industry research, with natural plant extracts as functional component
Pure natural cosmetic, because having higher traction and environmental friendliness, therefore becomes the trend of cosmetic product exploitation.
Publication No. has been the patented invention of CN104784069A, and a kind of fragrant plant with whitening antioxidation effect combines
Thing, fragrant plant compositionss used are prepared from by lemon grass (Cymbopogon citratus), Herba Menthae, Flos Nelumbinises, Paeonia suffruticosa, Flos Rosae Rugosae, have good white-skinned face function
With hydroxyl radical free radical Scavenging activity, and give cosmetics natural aromatic taste.
The patent for CN105434239A for the publication No. provides a kind of skin lightening compositions containing natural botanical source and exists
Application in cosmetics, described whitening active group is divided into Radix Rhodiolae extract and Folium Mori extract, safe to the human body, to tyrosine
Enzymatic activity has inhibitory action.
At present, consumer has the cosmetics of the anti-ageing effect of whitening for natural component there is still a demand.
Content of the invention
In view of this, the invention provides a kind of anti-ageing compositionss of whitening and cosmetics.The anti-ageing compositionss of this whitening can
Effectively suppression tyrosinase activity, has stronger antioxidation, has remarkable efficacy at the anti-ageing aspect of whitening.
In order to realize foregoing invention purpose, the present invention provides technical scheme below:
The invention provides a kind of anti-ageing compositionss of whitening, extracted by Amelanchier alnifolia extract, green tea extract, Herba Centellae
Thing and Huai Goat composition.
Amelanchier alnifolia is a kind of defoliation small arbor of Rosaceae Amelanchier or shrub, originates in Canada and the U.S., its fruit
For melonidum shape berry, rich in nutrition content, calcium content occupies first of BAIGUO, 88 milligrams of every 100g fresh fruit calcic, and containing substantial amounts of
Human body total dietary fiber required daily, each biostearin and the mineral such as necessary potassium, ferrum, magnesium, manganese, are in addition also rich in 18
Plant aminoacid.Either outward appearance, taste are still become to be grouped into and all be there is certain similarity with blue berry.According to one, North America biology
The research report of extraction company, the antioxidant tea polyphenols containing in Amelanchier alnifolia and the contents level of nutrient for plants and indigo plant
Quite, the total phenol content in every 100g is 452mg for the certain kind of berries, Pericarpium Citri tangerinae, Polyphenols that it contains, procyanidin, anthocyanidin, chlorogenic acid etc.
Antioxidant is all in terms of the sickness rate reducing multiple diseases such as cancer, heart disease, diabetes and auxiliary carry out clinical treatment
Show preferable effect, and also containing abundant pectin, pectin is the main component of dietary fiber, has good anti-abdomen
Rush down, anticancer, treatment diabetes and fat-reducing the effects such as.The product of Amelanchier alnifolia has reached tens kinds at present, including wine brewing, eats fruit raw
Product, medical, the senior beverage of health care etc., but it is not directed to cosmetic field.
Green tea contains abundant tea polyphenols, and tea polyphenols are the compounds that a class is present in the many antelopes base phenols in Camellia sinensis, its
Main component is catechin flavanol compound, pyrite and flavonols, anthocyan and phenolic acid and the many compounds of depside
Complex.Tea polyphenols belong to polyphenol structure, can go to live in the household of one's in-laws on getting married conjunction calcium, copper, 10 metal ion species such as ferrum, by copper ion chelating effect,
And suppress the oxidation of the low density lipoprotein, LDL of copper catalysis, thus ensure that the SOD not interfered with certain limit centered on copper lives
Property, and then suppress tyrosinase activity to reduce melanic generation.In addition, people can produce in metabolic process of life being harmful to freely
Base, the extremely strong oxidability of free radical can aoxidize unsaturated fatty acid and form lipid peroxide, and the meeting of accumulation slackens biomembranous
Normal function, the metabolism of impact active substance, and tea polyphenols have multiple ortho positions phenol antelope based structures, because polyphenol township base stage is easily by oxygen
Turn to awake class and provide proton to expand, therefore have stronger non-oxidizability.Meanwhile, tea polyphenols itself generate in the middle of stable free radical
Body, suppresses original free radical chain reactionses, thus protecting cell component injury-free, compared with other antioxidants, tea is many
Phenol scavenging activated oxygen has high efficiency, and compared with free radical scavenger, it is micro- that the efficiency of tea polyphenols scavenging activated oxygen is equivalent to 9
Gram Cu, Zn-SOD.Its scavenging activated oxygen, the efficiency of hydroxyl radical free radical want high several times compared with VC, VE.Furthermore, tea polyphenols
There is fabulous cooperative effect with VC, VE.It can produce and protect internal VC, VE, improves the content of internal VC, VE, Er Qiecha
Polyphenol may remove free radical with water solublity and fat-soluble two ways simultaneously, more more effective than single reducing agent.Tea polyphenols are also
There is radiation resistance and anti-allergic effects, absorbable radioactive substance, be referred to as natural ultraviolet filter.Also can pass through
During the scorching disease of suppression, the inflammatory cytokine of excessive secretion plays Hang Yan effect.Actually rare have huge potentiality
Skin-whitening agents.Publication No. is mentioned in the patent for CN101491490A when green tea extract concentration is 1mg/mL, and it is right
The suppression ratio of tryrosinase is 85%, and the IC50 value of its tyrosinase inhibition rate is 0.101mg/mL, and this shows that green tea extracts
Thing has significant inhibitory action to the activity of tryrosinase.
Herba Centellae is dried herb or whole herb with root, all herbal medicine for samphire Herba Centellae, can adopt throughout the year.In many
The existing thousand of years history of application in the field of medicaments of country.Its beginning is loaded in《Sheng Nong's herbal classic》, it is classified as middle product.It is cold in nature, taste storehouse,
Pungent, there is the effect of clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling, be mainly used in jaundice due to damp-heat, heatstroke is suffered from diarrhoea, sand Stranguria stranguria with blood, the swollen sore of pain, falls
Flutter damage.Herba Centellae extract contains multiple triterpeness, wherein comprises α-Amyrin structure, and main component is Asiaticoside,
Asiatic acid and Madecassic acid.Herba Centellae extract can improve enzyme type and non-enzyme type polyphenoils discrimination in newly-generated tissue
Change enzyme, hydrogen peroxide, the content of glutathione peroxidase, Vitamin E and ascorbic acid usp/bp, there is antioxidation, anti-ageing
Skin and complexion improving of waiting for a long time acts on.Herba Centellae extract is recommended the cicatrix regenerating for ageing skin to smooth by OTC skin nursing (can be in shaping
Post operation use).Herba Centellae can be compacted epidermis and corium coupling part, skin can be made to limber up, contribute to solving skin pine
Relaxation phenomenon, makes collagen protein in skin smooth flexible promotion skin corium be formed, so that fibrin is regenerated, reconnect is got up,
Skin is made to reach smooth effect of compacting.Also assist in impaired organization healing and consolidation skin.Epidermis and corium can be promoted
Between close connection, the increase of adipose cell can be suppressed again, prevent hydroderma, occur fat.Recent study finds long-pending
Snow grass has the effects such as antioxidation, antidepressant, liver protection, suppression tumor cell proliferation.
The Flos Sophorae Immaturus are the alabastrum of leguminous plant Chinese scholartree (Sophora japonica L.), existing more than 2000 year medicinal history,《God
Agriculture book on Chinese herbal medicine warp》Also it is classified as top grade.The composition such as rutin, Quercetin, triterpene saponin, polysaccharide lymphatic temperament, rutin is contained in the Flos Sophorae Immaturus
For main component, it is natural flavone compound, one of key component of Citrin, content is up to 12-20%.In recent years
Medical personal studies to Flos Sophorae Immaturus effect both at home and abroad, finds that its effective ingredient has anti-cancer and cancer-preventing, antiplatelet aggregation, town
Bitterly, the various active such as antibacterial, antiviral, defying age, antiinflammatory, antiallergic, free radical resisting and antioxidation, to improve myocardial circulation,
Heat-clearing and toxic substances removing, blood fat reducing, vessel softening, antiinflammatory the kidney invigorating etc. have good preventive and therapeutic effect, nontoxic to human body, harmless, no lethal, cause
Cancer, teratogenesis, mutagenic action.Rutin can strengthen the toughness of capillary tube, reduces capillary permeability and fragility, keep and recover
The normal elasticity of blood capillary, hyperpietic is had prevents rupture of blood vessel in brain effect, has stronger pharmacological action.And
Rutin has very strong Absorption to ultraviolet, and when addition is 10%, the absorbance of ultraviolet is up to 98%, has significantly
The effect of scavenger cell reactive oxygen free radical, is a kind of broad spectrum sunscreen ideal at present.
The present invention has the anti-ageing effect of significant whitening after using above-mentioned Four Plants extract combination, effect is better than respectively
Plant extract is used alone, and embodies obvious synergistic function.Said composition is applied in cosmetics, has complied with change
The trend of adornment industry, meets that consumer is natural to cosmetics, green, safe and efficient demand.
Preferably, in parts by weight, the anti-ageing compositionss of whitening include:1~6 part of Amelanchier alnifolia extract, green tea extracts
0.5~5 part of thing, 1~4 part of Herba Centellae extract, 0.8~3 part of Huai Goat.
In the embodiment that the present invention provides, in parts by weight, the anti-ageing compositionss of whitening include:Amelanchier alnifolia extract
1 part, 3 parts of green tea extract, 4 parts of Herba Centellae extract, 0.8 part of Huai Goat.
In another embodiment that the present invention provides, in parts by weight, the anti-ageing compositionss of whitening include:Amelanchier alnifolia extracts
3 parts of thing, 5 parts of green tea extract, 1 part of Herba Centellae extract, 2 parts of Huai Goat.
In another embodiment that the present invention provides, in parts by weight, the anti-ageing compositionss of whitening include:Amelanchier alnifolia extracts
6 parts of thing, 0.5 part of green tea extract, 3 parts of Herba Centellae extract, 3 parts of Huai Goat.
Preferably, Amelanchier alnifolia extract is Amelanchier alnifolia ethanol extract.
Preferably, the preparation method of Amelanchier alnifolia ethanol extract is:By being crushed to 180 after Amelanchier alnifolia clean dry~
200 mesh, add the ethanol that the concentration of volume percent being equivalent to 8~20 times of raw material weight is 40%~90%, at 55~85 DEG C
Extract 2~4 times, 1~3h every time, united extraction liquid, filter, filtrate reduced in volume, be dried, obtain Amelanchier alnifolia ethanol extract.
In the embodiment that the present invention provides, the preparation method of Amelanchier alnifolia ethanol extract is:By Amelanchier alnifolia clean dry
After be crushed to 180 mesh, add the ethanol that the concentration of volume percent being equivalent to 15 times of raw material weight is 75%, at 70 DEG C, extract 3
Secondary, each 2h, united extraction liquid, filters, filtrate reduced in volume, is dried, obtains Amelanchier alnifolia ethanol extract.
Present invention also offers a kind of cosmetics, including Amelanchier alnifolia extract, green tea extract, Herba Centellae extract,
Acceptable adjuvant on Huai Goat and cosmeceutical.
Preferably, on cosmeceutical acceptable adjuvant to include propylene glycol, emollient, wetting agent, thickening agent, spermaceti hard
Lipidol, cetearyl glucoside, Glyceryl stearate citrate, caprylyl glycol, Sensiva SC50, MEIGUI CHUNLU, EDTA-
2Na, pH adjusting agent and water.
Preferably, in terms of weight/mass percentage composition, in cosmetics, each amounts of components is:
Preferably, emollient is Semen Tritici aestivi germ oil, macadimia nut oil, Jojoba oil, American Avocado Tree oil, shea butter, sweet Fructus Pruni
One or more of core oil, Semen Coicis oil, Testa oryzae oil, safflower oil or myristyl alcohol myristinate.
Preferably, emollient is Semen Tritici aestivi germ oil, macadimia nut oil, Jojoba oil, American Avocado Tree oil, shea butter, Semen pruni armeniacae
Two or more in oil, Semen Coicis oil, Testa oryzae oil, safflower oil or myristyl alcohol myristinate.
Preferably, wetting agent be ethoxy urea, ceramide, hyaluronate sodium, levan, allantoin, reed fragrant plant oil, mountain
One or more of pears sugar alcohol or glycine betaine.
Preferably, thickening agent is Carbomer, xanthan gum, alginate jelly, guar gum, shitosan, arabic gum or Caesalpinia spinosaKuntze
One or more of glue.
Preferably, pH adjusting agent is one or two of citric acid or triethanolamine.
Preferably, the pH value of cosmetics of the present invention is 5~7.
Present invention also offers the preparation method of this cosmetics, comprise the steps:
By emollient, cetearyl alcohol, cetearyl glucoside, Glyceryl stearate citrate mixing, it is heated to
80~85 DEG C, stir to being completely dissolved, be incubated 5~10min, obtain oil phase;
By water, glycerol, thickening agent, caprylyl glycol, ethyl hexyl glycerol, EDTA-2Na mixing, it is heated with stirring to 80~85 DEG C, makes
It fully dissolves, and obtains aqueous phase;
Oil phase is mixed with water, homogenizing 2~5min, then it is incubated 30~35min;
When temperature is down to 40~45 DEG C, wetting agent, Amelanchier alnifolia extract, green tea extract, Herba Centellae is added to extract
Thing and Huai Goat, stir, and lower the temperature after insulation 10~15min;
When temperature is down to 15~30 DEG C, pH to 5~7 is adjusted using pH adjusting agent;
It is eventually adding MEIGUI CHUNLU, stand 12~24h after stirring, obtain cosmetics.
The invention provides a kind of anti-ageing compositionss of whitening and cosmetics.The anti-ageing compositionss of this whitening are extracted by Amelanchier alnifolia
Thing, green tea extract, Herba Centellae extract and Huai Goat composition.The present invention at least has one of following advantage:
1st, the selected natural extract composition from Four Plants of the functional component of the anti-ageing cosmetics of the whitening of the present invention, as alder
Leaf Chinese bush cherry extract, green tea extract, Herba Centellae extract and Huai Goat, can effectively suppress tyrosine after being applied in combination
Enzymatic activity, suppresses melanic generation, the notable removing machine interior free yl of energy, has stronger antioxidation, whitening is resisted
Decline and there is the effect of complementary Synergistic;
Amelanchier alnifolia extract contains the antioxidant composition such as abundant Polyphenols, procyanidin, anthocyanidin, chlorogenic acid,
Remove free radical, anti-inflammatory activity, the synthesis safeguarding collagen, the formation improving skin elasticity, suppression lipofuscin and senile plaque, minimizing
The aspects such as wrinkle generation, whitening sun protection have significant effect;Green tea extract can significantly inhibit the activity of tryrosinase, reduces
Melanic generation, can remove free radical by high-efficient cleaning, also have radioprotective and antianaphylactic effect;Herba Centellae extract can make skin
Limber up, contribute to solving cutis laxa phenomenon, make skin smooth flexible, can help promote collagen protein in skin corium to be formed
Regenerate with fibrin and eliminate microgroove, so that skin is compacted smooth, and the increase of adipose cell can be suppressed, prevent skin from edema occurring
And obesity;Huai Goat has very strong Absorption to ultraviolet, is a kind of natural broad spectrum sunscreen, and the Flos Sophorae Immaturus carry
Thing is taken also to have multiple effect such as antibacterial, antiviral, defying age, antiinflammatory, antiallergic, antioxidation, Red blood streak dispelling.
Compositionss and cosmetics that the present invention provides, natural safe and non-stimulating, there is good whitening and speckle dispelling, anti-ageing anti-
Wrinkle, the effect of the skin that compacts, and also have preferable water conservation humidification, sun-proof, Red blood streak dispelling effect to skin simultaneously, make for a long time
With skin can be made to assume health youth state.
2nd, the present invention does not contain preservative, but has antiseptic effect, and the caprylyl glycol being added has excellent broad-spectrum antiseptic and lives
Property, and Sensiva SC50 can substantially increase the antibiotic effect of caprylyl glycol, MEIGUI CHUNLU has antimicrobial component, by Synergistic
Effect, favorable anti-corrosion effect, and this three also has good skin moisture-keeping performance, and safe, non-stimulated.
3rd, the spice that the present invention adds is MEIGUI CHUNLU, and good water solubility not only can give the fragrance of product pleasure, and
Also have keep the skin wet, moisturizing, quick antiinflammatory, antiallergic, the skin care effect such as antipruritic, slow down aging, furthermore Sensiva SC50
Flavouring, fixation and surely fragrant efficiency can also be played.
4th, the functional component in the cosmetics of the present invention is composition of plant extracts, and natural safety is non-stimulated.
5th, the product preparation process of the present invention is simple, suitable commercial production.
Specific embodiment
The invention discloses a kind of anti-ageing compositionss of whitening and cosmetics, those skilled in the art can use for reference in herein
Hold, be suitably modified technological parameter and realize.Specifically, all similar replacements and change are to those skilled in the art
For be it will be apparent that they are considered as including in the present invention.Preferable enforcement has been passed through in the method for the present invention and application
Example is described, related personnel substantially can in without departing from present invention, spirit and scope to method described herein and
Application is modified or suitably changes and combine, and to realize and to apply the technology of the present invention.
In the present invention, the preparation method of Amelanchier alnifolia extract is as follows:After Amelanchier alnifolia clean dry, use pulverizer
Processed, be crushed to 180~200 mesh, take Amelanchier alnifolia powder to be placed in the body adding 8~20 times in the boiling flask of tool plug
Long-pending percent concentration is 40%~90% ethanol, extracts 2~4 times, 1~3h every time at 55~85 DEG C, merges each extracting solution,
Filtered with 0.45 μm of microporous filter membrane, filtrate reduced in volume, be then drying to obtain Amelanchier alnifolia extract.
In anti-ageing compositionss of whitening that the present invention provides and cosmetics, raw materials used or adjuvant all can be buied by market.
In the following embodiments, the preparation method of Amelanchier alnifolia extract is as follows:After Amelanchier alnifolia clean dry, use powder
Broken machine is processed, and is crushed to 180 mesh, takes Amelanchier alnifolia powder to be placed in the volume hundred adding 15 times in the boiling flask of tool plug
Divide the ethanol that specific concentration is 75%, extract 3 times at 55-85 DEG C, each 2h, merge each extracting solution, with 0.45 μm of microporous filter membrane
Filtered, filtrate reduced in volume, be then drying to obtain Amelanchier alnifolia extract.
This 3 kinds of extracts of green tea extract, Herba Centellae extract and Huai Goat are all commercially.Wherein, green
Tea extract, Herba Centellae extract are purchased from Guangzhou Xu Fan trade Co., Ltd, and Huai Goat is purchased from Guangzhou card sweet smell biotechnology
Company limited.
With reference to embodiment, the present invention is expanded on further:
The preparation of the anti-ageing compositionss of embodiment 1 whitening
The anti-ageing compositionss of the present embodiment whitening are mixed to prepare by following plant extract:
The preparation of the anti-ageing compositionss of embodiment 2 whitening
The anti-ageing compositionss of the present embodiment whitening are mixed to prepare by following plant extract:
The preparation of the anti-ageing compositionss of embodiment 3 whitening
The anti-ageing compositionss of the present embodiment whitening are mixed to prepare by following plant extract:
The anti-ageing efficacy test of whitening of test example 1 compositionss
First, external whitening test
1st, test objective:Carry out tryrosinase inhibition to compare.
2nd, test apparatuses and reagent
Key instrument:Spectrophotometer Spectronic GENESYS-5, Thermo company of the U.S.;
Main agents:Tryrosinase (sigma company of the U.S.), other reagent are conventional reagent;The embodiment of the present invention 1 combines
Thing, embodiment 2 compositionss, embodiment 3 compositionss, Amelanchier alnifolia extract, green tea extract, Herba Centellae extract and the Flos Sophorae Immaturus carry
Take thing, the concentration of preparation is 6%.
3rd, test method
It is separately added into phosphate buffer (pH6.8,0.1mol/L), sample liquid, tyrosine solution in each test tube.It is placed in 37
10min in DEG C water-bath, is subsequently adding tryrosinase, reacts 10min, survey absorbance in 475nm, averagely in 37 DEG C of water-baths
Experiment 3 times, seeks its meansigma methods.
It is blank that experimental system design is shown in Table 1, C1, and C2 is used for blank zeroing, and S1 is sample cell, and S2 is used for sample and adjusts
Zero.Sample is embodiment 1, embodiment 2, embodiment 3, Amelanchier alnifolia extract, green tea extract, Herba Centellae extract and the Flos Sophorae Immaturus
Extract.
Tyrosinase inhibition rate=(blank absorbance-sample absorbance value)/blank absorbance ×
100%
Table 1 tryrosinase Inhibition test system is loaded table
Reagent |
C1/mL |
C2/mL |
S1/mL |
S2/mL |
PBS |
2 |
2.5 |
1 |
1.5 |
Sample |
0 |
0 |
1 |
1 |
L-Tyrosine |
1 |
1 |
1 |
1 |
Tyrosinase solution |
0.5 |
0 |
0.5 |
0 |
4th, result of the test is shown in Table 2.
Table 2 tyrosinase inhibition rate measures
Sample |
Suppression ratio (%) |
Embodiment 1 compositionss |
91.7 |
Embodiment 2 compositionss |
93.5 |
Embodiment 3 compositionss |
88.1 |
Amelanchier alnifolia extract |
78.4 |
Green tea extract |
80.6 |
Herba Centellae extract |
69.5 |
Huai Goat |
75.8 |
In vitro tests result shows, embodiment of the present invention 1-3 compositionss have preferable tyrosinase inhibitory activity, suppression
Rate is higher than each single dose group.
2nd, antioxidation in vitro test
Experiment purpose:With in vitro tests, the antioxidant effect of plant extract compositionss is investigated.
Experiment material:Free radical scavenging activity is (referred to as:DPPH, Sigma company, the U.S.);Du800 UV, visible light
Spectrophotometer (Beckman company, the U.S.);The compositionss of preparation in embodiment 1-3, Amelanchier alnifolia extract, green tea extracts
Thing, Herba Centellae extract, Huai Goat, the concentration of preparation is 6%.
Experimental technique:Take 0.1mL sample solution, add the DPPH solution of 60 μm of ol/L of 2mL to mix, place 30min, with
Former solvent zeroising, having surveyed absorbance at 515nm is Ai;Mix mensuration absorbance with method 0.1mL solvent+2mL DPPH solution
Extremely Ac;0.1mL sample solution+2mL solvent mixes mensuration absorbance extremely Aj.Its free radical scavenging according to the following formula
Rate.
Clearance rate (%)=[1- (Ai-Aj)/Ac] × 100%
Wherein, the contribution to absorbance for the Aj response sample itself;Ai sample is to the absorption values after DPPH effect;Ac is
DPPH is originally in the absorption measuring wavelength.
Experimental result is as follows:
Table 3 plant extract compositionss oxidation resistance is tested
Sample |
Clearance rate (%) |
Embodiment 1 compositionss |
90.7 |
Embodiment 2 compositionss |
94.8 |
Embodiment 3 compositionss |
96.2 |
Amelanchier alnifolia extract |
84.9 |
Green tea extract |
80.3 |
Herba Centellae extract |
78.8 |
Huai Goat |
82.5 |
Vitro Experimental Results show, embodiment of the present invention 1-3 compositionss have preferable radical scavenging activity, clearance rate
Higher than each single dose group.
The preparation of the anti-ageing cosmetics of embodiment 4 whitening
The anti-ageing cosmetics of whitening containing various plants extract manufactured in the present embodiment, including the one-tenth of following weight proportion
Point:
The step preparing above-mentioned cosmetic composition:
Emollient, cetearyl alcohol, cetearyl glucoside, Glyceryl stearate citrate are heated to 80 DEG C,
Stir to being completely dissolved, be incubated 10min, as oil phase;
By water, glycerol, thickening agent, caprylyl glycol, ethyl hexyl glycerol, EDTA-2Na mixing, it is heated with stirring to 80 DEG C so as to fill
Divide and be dissolved to clear, as aqueous phase;
Then while stirring oil phase is added aqueous phase, homogenizing 2min, be then incubated 35min;
When temperature is down to 40 DEG C, sequentially add wetting agent, Amelanchier alnifolia extract, green tea extract, Herba Centellae extraction
Thing and Huai Goat, stir, and lower the temperature after insulation 15min;
When temperature is for room temperature, it is slowly added to pH adjusting agent and adjusts pH to 5;It is eventually adding MEIGUI CHUNLU, after stirring
Standing 12h obtains final product.
The preparation of the anti-ageing cosmetics of embodiment 5 whitening
The anti-ageing cosmetics of whitening containing various plants extract manufactured in the present embodiment, including the one-tenth of following weight proportion
Point:
The step preparing above-mentioned cosmetic composition:
Emollient, cetearyl alcohol, cetearyl glucoside, Glyceryl stearate citrate are heated to 82 DEG C,
Stir to being completely dissolved, be incubated 8min, as oil phase;
By water, glycerol, thickening agent, caprylyl glycol, ethyl hexyl glycerol, EDTA-2Na mixing, it is heated with stirring to 82 DEG C so as to fill
Divide and be dissolved to clear, as aqueous phase;
Then while stirring oil phase is added aqueous phase, homogenizing 3min, be then incubated 32min;
When temperature is down to 42 DEG C, sequentially add wetting agent, Amelanchier alnifolia extract, green tea extract, Herba Centellae extraction
Thing and Huai Goat, stir, and lower the temperature after insulation 12min;
When temperature is for room temperature, it is slowly added to pH adjusting agent and adjusts pH to 6;It is eventually adding MEIGUI CHUNLU, after stirring
Standing 20h obtains final product.
The preparation of the anti-ageing cosmetics of embodiment 6 whitening
The anti-ageing cosmetics of whitening containing various plants extract manufactured in the present embodiment, including the one-tenth of following weight proportion
Point:
The step preparing above-mentioned cosmetic composition:
Emollient, cetearyl alcohol, cetearyl glucoside, Glyceryl stearate citrate are heated to 85 DEG C,
Stir to being completely dissolved, be incubated 5min, as oil phase;
By water, glycerol, thickening agent, caprylyl glycol, ethyl hexyl glycerol, EDTA-2Na mixing, it is heated with stirring to 85 DEG C so as to fill
Divide and be dissolved to clear, as aqueous phase;
Then while stirring oil phase is added aqueous phase, homogenizing 5min, be then incubated 30min;
When temperature is down to 45 DEG C, sequentially add wetting agent, Amelanchier alnifolia extract, green tea extract, Herba Centellae extraction
Thing and Huai Goat, stir, and lower the temperature after insulation 10min;
When temperature is for room temperature, it is slowly added to pH adjusting agent and adjusts pH to 7;It is eventually adding MEIGUI CHUNLU, after stirring
Standing 24h obtains final product.
Test example 2 cosmetic effect is tested
First, acute dermal toxicity test
24h before test, SD rat back spinal column diamond wool is cut, and cropping area is no less than the 10% of body surface area.By 2
The rat random packet of kind of sex, every group 6, dosage is grouped into 500,1000,1500,2000,2500mg/kg.By the present invention
The cosmetics of embodiment 4 are uniformly applied to rat back, and are covered with oilpaper and 2 layers of gauze, remove residual with warm water after 24h
Thing.Observe systemic toxicity profiles performance and the death condition of rat, including the change of rat skin, hair, eyes and mucosa, breathe,
The change of extremity activity and way of act etc., observes 1 week.Result is as follows:
Smear this cosmetics and poisoning and death condition did not occurred after 1 week, skin, hair and eyes exception all, not
The phenomenons such as appearance is trembled, faints from fear, sialorrhea, diarrhoea, drowsiness, stupor, illustrate the no any harmful effect to its skin of this cosmetics.And
And in this test, maximum dose level group is up to 2500mg/kg, rats death does not occur it may be determined that its median lethal dose(LD 50) is long-range
In 2500mg/kg.So determining that this cosmetics reality is nontoxic.
Cosmetics using embodiment 5 or 6 carry out acute dermal toxicity test, poisoning and death after smearing 1 week
Situation, all exception in skin, hair and eyes, do not occur trembling, faint from fear, sialorrhea, diarrhoea, the phenomenon such as drowsiness, stupor, say
This cosmetics bright no any harmful effect to its skin.
2nd, Efficiency assessment
Trier is the healthy women of voluntary participation, is by age divided into 2 groups, 20-35 year be A group, 35-55 year be B group,
Several 16 people of each group of people, wherein 8 people are professional person.Every trier uses the cosmetics one month of the embodiment of the present invention 4, daily
Once.Occupation mode is:By this face after cleaning for cosmetics uniform application.Observe and investigate trial effect, fragrant from product
Type, product skin sense, whitening capability, anti-ageing ability, Firm ability, six aspects of moisture-retaining capacity are commented to the cosmetics of the present invention
Valency, result is as shown in table 4.
The trial effect of all volunteers of table 4
Project |
Well (%) |
Typically (%) |
Poor (%) |
Product odor type |
100 |
0 |
0 |
Product skin sense |
94 |
6 |
0 |
Whitening capability |
87 |
13 |
0 |
Anti-ageing ability |
91 |
6 |
3 |
Firm ability |
88 |
9 |
3 |
Moisture-retaining capacity |
94 |
6 |
0 |
Result on trial:After one month, all anaphylaxiss in all of trier, unanimously thinks that this product safety is stingless
Swash, have good improvement result to skin quality, whitening and speckle dispelling, anti-ageing anti-wrinkle effect substantially, also have water conservation humidification, sun-proof, dispel red
The effect of the blood streak, makes skin smooth compact high resilience.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.