CN106460023B - 使用α-葡糖苷酶来酶水解二糖和低聚糖 - Google Patents

使用α-葡糖苷酶来酶水解二糖和低聚糖

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Publication number
CN106460023B
CN106460023B CN201580010439.5A CN201580010439A CN106460023B CN 106460023 B CN106460023 B CN 106460023B CN 201580010439 A CN201580010439 A CN 201580010439A CN 106460023 B CN106460023 B CN 106460023B
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China
Prior art keywords
alpha
glucan
glucosidase
fraction
synthesis reaction
Prior art date
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Active
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CN201580010439.5A
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English (en)
Chinese (zh)
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CN106460023A (zh
Inventor
K.D.纳盖
E.C.哈戈
J.K.舍蒂
S.M.亨内塞
R.迪科斯莫
L.华
R.拉米雷兹
Z.汤
Z.于
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DuPont Industrial Biosciences USA LLC
Nutrition and Biosciences USA 4 Inc
Original Assignee
EI Du Pont de Nemours and Co
Nutrition and Biosciences USA 4 Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/08—Dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01005—Dextransucrase (2.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01024—1,4-Alpha-glucan 6-alpha-glucosyltransferase (2.4.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0107—Glucan 1,6-alpha-glucosidase (3.2.1.70)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01084—Glucan 1,3-alpha-glucosidase (3.2.1.84), i.e. mutanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01115—Branched-dextran exo-1,2-alpha-glucosidase (3.2.1.115)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201580010439.5A 2014-02-27 2015-02-26 使用α-葡糖苷酶来酶水解二糖和低聚糖 Active CN106460023B (zh)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US201461945233P 2014-02-27 2014-02-27
US201461945241P 2014-02-27 2014-02-27
US61/945241 2014-02-27
US61/945233 2014-02-27
US201462004308P 2014-05-29 2014-05-29
US201462004305P 2014-05-29 2014-05-29
US201462004312P 2014-05-29 2014-05-29
US201462004300P 2014-05-29 2014-05-29
US201462004290P 2014-05-29 2014-05-29
US201462004314P 2014-05-29 2014-05-29
US62/004290 2014-05-29
US62/004300 2014-05-29
US62/004305 2014-05-29
US62/004314 2014-05-29
US62/004312 2014-05-29
US62/004308 2014-05-29
CN2015073269 2015-02-25
CNPCT/CN2015/073269 2015-02-25
PCT/US2015/017648 WO2015130883A1 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

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CN106460023A CN106460023A (zh) 2017-02-22
CN106460023B true CN106460023B (zh) 2026-02-24

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CN201580010441.2A Pending CN106068327A (zh) 2014-02-27 2015-02-26 使用α‑葡糖苷酶来酶水解二糖和低聚糖

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EP (2) EP3110959A1 (cg-RX-API-DMAC10.html)
JP (2) JP7011393B2 (cg-RX-API-DMAC10.html)
CN (2) CN106460023B (cg-RX-API-DMAC10.html)
AU (2) AU2015223025B2 (cg-RX-API-DMAC10.html)
BR (1) BR112016019823B1 (cg-RX-API-DMAC10.html)
CA (2) CA2940778A1 (cg-RX-API-DMAC10.html)
WO (2) WO2015130881A1 (cg-RX-API-DMAC10.html)

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BR112017013368B1 (pt) 2014-12-22 2021-11-23 Nutrition & Biosciences USA 4, Inc. Composição de mistura polimérica
EP3253454B1 (en) 2015-02-06 2020-04-22 DuPont Industrial Biosciences USA, LLC Colloidal dispersions of poly alpha-1,3-glucan based polymers
EP3262180A1 (en) * 2015-02-25 2018-01-03 Danisco US Inc. Alpha-glucosidase, compositions & methods
JP6956013B2 (ja) * 2015-06-01 2021-10-27 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド ポリα−１，３−グルカンフィブリッド及びその使用、並びにポリα−１，３−グルカンフィブリッドを製造する方法
WO2016196021A1 (en) 2015-06-01 2016-12-08 E I Du Pont De Nemours And Company Structured liquid compositions comprising colloidal dispersions of poly alpha-1,3-glucan
EP3368717B1 (en) 2015-10-26 2022-12-14 Nutrition & Biosciences USA 4, Inc. Polysaccharide coatings for paper
CN108350660B (zh) 2015-10-26 2022-04-29 营养与生物科学美国4公司 水不溶性α-(1，3→葡聚糖)组合物
WO2017083226A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
WO2017083228A1 (en) * 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
WO2017083229A1 (en) * 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
US10266861B2 (en) * 2015-12-14 2019-04-23 E. I. Du Pont De Nemours And Company Production and composition of fructose syrup
US10895028B2 (en) 2015-12-14 2021-01-19 Dupont Industrial Biosciences Usa, Llc Nonwoven glucan webs
CN110291202B (zh) * 2016-12-15 2023-11-28 丹尼斯科美国公司 用于在淀粉水解过程中增加由玉米纤维进行的乙醇的生产的方法
JP2018139554A (ja) * 2017-02-28 2018-09-13 日本食品化工株式会社 水溶性食物繊維含有組成物及びその製造方法
CN110651049A (zh) * 2017-05-23 2020-01-03 纳幕尔杜邦公司 α-1,3-葡聚糖的酶促生产
EP3415622A1 (en) * 2017-06-14 2018-12-19 Evonik Degussa GmbH Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases
EP3668970A1 (en) * 2017-09-13 2020-06-24 DuPont Industrial Biosciences USA, LLC Engineered glucosyltransferases
CN111566128B (zh) * 2017-11-10 2022-06-21 营养与生物科学美国4公司 独特形态的多糖
US10808269B2 (en) * 2018-02-23 2020-10-20 Danisco Us Inc Synthesis of glucan comprising alpha-1,3 glycosidic linkages with phosphorylase enzymes
CN115197884A (zh) * 2022-08-24 2022-10-18 吉林大学 一种降解废弃淀粉的微生物菌剂及其应用方法
CN120118963A (zh) * 2023-12-08 2025-06-10 宁夏伊品生物科技股份有限公司 aglA基因在制备L-氨基酸中的应用

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BR112016019820A2 (pt) 2017-10-17
AU2015223025A1 (en) 2016-08-04
EP3110958A1 (en) 2017-01-04
AU2015223027A1 (en) 2016-08-04
WO2015130883A1 (en) 2015-09-03
CA2940778A1 (en) 2015-09-03
WO2015130883A9 (en) 2016-08-18
WO2015130881A1 (en) 2015-09-03
AU2015223027B2 (en) 2018-09-20
BR112016019823A2 (pt) 2017-10-17
JP2017518025A (ja) 2017-07-06
EP3110959A1 (en) 2017-01-04
AU2015223025B2 (en) 2018-09-20
JP6929499B2 (ja) 2021-09-01
CN106068327A (zh) 2016-11-02
JP7011393B2 (ja) 2022-02-10
JP2017518026A (ja) 2017-07-06
CN106460023A (zh) 2017-02-22
BR112016019823B1 (pt) 2023-05-09
CA2938144A1 (en) 2015-09-03

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