CN106432479B - 一种从牛皮中提取胶原蛋白的方法 - Google Patents
一种从牛皮中提取胶原蛋白的方法 Download PDFInfo
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- CN106432479B CN106432479B CN201611032604.XA CN201611032604A CN106432479B CN 106432479 B CN106432479 B CN 106432479B CN 201611032604 A CN201611032604 A CN 201611032604A CN 106432479 B CN106432479 B CN 106432479B
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- 238000000034 method Methods 0.000 title claims abstract description 32
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 42
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 28
- 238000003756 stirring Methods 0.000 claims description 28
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 21
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 21
- 102000004190 Enzymes Human genes 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 17
- 238000005237 degreasing agent Methods 0.000 claims description 16
- 239000013527 degreasing agent Substances 0.000 claims description 16
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 14
- 235000019441 ethanol Nutrition 0.000 claims description 14
- 239000000706 filtrate Substances 0.000 claims description 14
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- 238000004064 recycling Methods 0.000 claims description 9
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- 239000000243 solution Substances 0.000 claims description 8
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- 239000012634 fragment Substances 0.000 claims description 7
- 235000013372 meat Nutrition 0.000 claims description 7
- 239000002504 physiological saline solution Substances 0.000 claims description 7
- 230000001105 regulatory effect Effects 0.000 claims description 7
- 238000007920 subcutaneous administration Methods 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 6
- 102000013392 Carboxylesterase Human genes 0.000 claims description 4
- 108010051152 Carboxylesterase Proteins 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 101100333714 Drosophila melanogaster hay gene Proteins 0.000 claims description 3
- 102000004157 Hydrolases Human genes 0.000 claims description 3
- 108090000604 Hydrolases Proteins 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- 229930182558 Sterol Natural products 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- 235000003702 sterols Nutrition 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 150000003432 sterols Chemical class 0.000 claims description 2
- 239000002994 raw material Substances 0.000 abstract description 3
- 238000007796 conventional method Methods 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 description 6
- 230000006837 decompression Effects 0.000 description 4
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- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 235000014103 egg white Nutrition 0.000 description 2
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- 108050001049 Extracellular proteins Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
Description
对比项 | 胶原蛋白(%) |
实施例1 | 74.8 |
实施例2 | 75.1 |
实施例3 | 75.6 |
实施例4 | 75.2 |
实施例5 | 74.9 |
实施例6 | 75.1 |
实施例7 | 41.3 |
实施例8 | 55.6 |
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201611032604.XA CN106432479B (zh) | 2016-11-16 | 2016-11-16 | 一种从牛皮中提取胶原蛋白的方法 |
Applications Claiming Priority (1)
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CN201611032604.XA CN106432479B (zh) | 2016-11-16 | 2016-11-16 | 一种从牛皮中提取胶原蛋白的方法 |
Publications (2)
Publication Number | Publication Date |
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CN106432479A CN106432479A (zh) | 2017-02-22 |
CN106432479B true CN106432479B (zh) | 2019-10-22 |
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CN201611032604.XA Active CN106432479B (zh) | 2016-11-16 | 2016-11-16 | 一种从牛皮中提取胶原蛋白的方法 |
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CN (1) | CN106432479B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107653291A (zh) * | 2017-11-15 | 2018-02-02 | 西藏央金生态农牧科技有限公司 | 多步酶法协同制备藏牦牛皮胶原蛋白和胶原多肽的方法 |
CN109568664A (zh) * | 2018-11-29 | 2019-04-05 | 薛志强 | 一种用于鼻部填充整形的肋软骨材料制备方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105061588A (zh) * | 2015-08-27 | 2015-11-18 | 长春博泰医药生物技术有限责任公司 | 制备胶原蛋白的牛皮处理方法 |
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2016
- 2016-11-16 CN CN201611032604.XA patent/CN106432479B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105061588A (zh) * | 2015-08-27 | 2015-11-18 | 长春博泰医药生物技术有限责任公司 | 制备胶原蛋白的牛皮处理方法 |
Non-Patent Citations (3)
Title |
---|
枯草杆菌脂肪水解酶LipA 和LipB;赵博;《中国生物化学与分子生物学报》;20080531;419-425 * |
皮胶原的结构、性质与提取方法;王娜等;《皮革科学与工程》;20060430;第16卷(第2期);42-46 * |
酸法制备牛皮胶原蛋白及其结构性质研究;沈菊泉等;《食品工业》;20091231;43-46 * |
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