CN106165607A - A kind of detoxification business's potato 19 rapid propagation method - Google Patents

A kind of detoxification business's potato 19 rapid propagation method Download PDF

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Publication number
CN106165607A
CN106165607A CN201610632450.1A CN201610632450A CN106165607A CN 106165607 A CN106165607 A CN 106165607A CN 201610632450 A CN201610632450 A CN 201610632450A CN 106165607 A CN106165607 A CN 106165607A
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China
Prior art keywords
potato
seedling
business
seed
detoxification
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CN201610632450.1A
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Chinese (zh)
Inventor
王立国
郭子江
张嘉
李建军
朱永伶
芮淑会
王鸿鸣
李彦阳
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Tianjin Fenghua Yulong Agricultural Development Co Ltd
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Tianjin Fenghua Yulong Agricultural Development Co Ltd
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Priority to CN201610632450.1A priority Critical patent/CN106165607A/en
Publication of CN106165607A publication Critical patent/CN106165607A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention is a kind of detoxification business's potato 19 rapid propagation method, concretely comprises the following steps: plant or tuber grown on top point are cut, and carries out tissue culture, obtains without Solanum carolinense Seedling；Select high-land, leeward on the sunny side, near water source, rich in organic chesson as seedbed；Selected potato seed, potato block pathogenic bacteria of going out before row's potato；During seeding, making potato seed not expose, then tile mulch film, heat and moisture preserving；Should heat in advance before potato seed discharge, during nursery, be strictly on guard against low temperature, in order to avoid potato Seedling produces freeze injury；After potato bud is unearthed, throw off mulch film, it is to avoid burn seedlings；It is planted to collect seedling in garden by short Seedling, strengthens rich water quality management, be enlarged breeding, after growing up to potato Seedling, be transplanted to big field as seedling.The invention provides a kind of detoxification business's potato 19 rapid propagation method, solve detoxification business's potato 19 breeds supply problem, achieves good economic benefit, and effect of increasing production is obvious.

Description

A kind of detoxification business's potato 19 rapid propagation method
Technical field
The present invention relates to the fast numerous field of Rhizoma Dioscoreae esculentae, particularly relate to a kind of detoxification business's potato 19 rapid propagation method.
Background technology
Detoxification business's potato 19 is germinating good, and stem and leaf growth is vigorous, knot potato early, potato block neatly concentrate, yield is high, quality is excellent, resistance to Storage, commodity property is good, high financial profit.And breed detoxification business's potato 19 potato seed, seedling requirement possesses certain condition and quite Technical merit, the only minority R&D institution that strong technical strength just can undertake this task.But, these R&D institutions one As be all built up in big and medium-sized cities, away from producing land for growing field crops, directly carry out from these R&D institutions allocation and transportation detoxification business's potato 19 potato seed, seedling Production of Large Fields is the most unrealistic.And detoxification business's potato 19 breeding can only use 2 years continuously, within the 3rd year, become commodity potato, it is impossible to again Make potato seed.Detoxification business potato 19 individual plant knot potato is few simultaneously, and potato seed breeding coefficient is low, thus popularization and application exist certain tired aborning Difficult.
Summary of the invention
Present invention seek to address that the deficiencies in the prior art, and a kind of detoxification business's potato 19 rapid propagation method is provided.
The present invention for achieving the above object, by the following technical solutions:
A kind of detoxification business's potato 19 rapid propagation method, concretely comprises the following steps:
(1) without the acquisition of Solanum carolinense Seedling:
Business's potato 19 shoot tip meristem virus concentration is low, and have does not even contain virus, by band poison plant or tuber grown on top Point cuts, and carries out tissue culture, obtains without Solanum carolinense Seedling；
(2) selection in seedbed:
Select high-land, leeward on the sunny side, near water source, rich in organic matter, 3-5 did not plant business's potato 19, and around 600-800m not 19 kinds of chessons planted of band poison business potato are as seedbed；The thin rake of deep ploughing before nursery, farm manure is executed in every mu of seedbed 4500-4800kg, carbon ammonium 30-40kg, make the furrow face that width is 1.2m；
(3) selected potato seed:
Epidermis potato seed smooth, anosis, without wound, without freeze injury is selected to carry out seeding；With the water seed soaking of 55 DEG C before row's potato 13min, or by 500 times of immersion kinds 7-8min of 70% thiophanate methyl, to kill potato block pathogenic bacteria；
(4) seeding:
The seeding time is early Febuary or the middle ten days, is put by seedbed table mound before seeding, pads one layer of abundant corruption at the bottom of bed Ripe pigs and cattle hurdle excrement, then repaves table soil thick for last layer 26-30cm；To distinguish end to end during seeding, it is impossible to the row of falling；Potato seed is by big Little separately, yaw is dilute puts, and is pressed in by the head of second piece of potato seed at the 1/3 of first piece of potato seed afterbody, potato seed left and right Ge Liu 1-2cm Space；1m2The flow rate in seedbed is 21kg；After drained kind, loam calking, water permeable, cover table soil thick for 2-3cm immediately, Making potato seed not expose, then tile mulch film, heat and moisture preserving；
(5) seedbed management:
Detoxification business's potato 19 nursery uses heating type hot house nursery on the ground, should heat in advance, make seedbed before potato seed discharge Temperature reaches more than 29 DEG C, and in nursery the 1st week, nursery bed temperature controls, at 36 DEG C, to be gradually decreased to 31 DEG C later, Seedling all sprout after temperature Control at 26-27 DEG C；Low temperature it is strictly on guard against, in order to avoid potato Seedling produces freeze injury during nursery；After potato bud is unearthed, throw off mulch film, it is to avoid burn seedlings； After young leaves starts to spread out, open thin film time when the fine day morning 10 to afternoon 3 and ventilate；Cutting 3-4d dry in the sun on daytime before Seedling, cover in the evening Film, ventilation and penetrating light seedling exercising；
(6) expanding propagation:
When seedbed potato Seedling length is to 11-15 leaf, potato Seedling is cut into the short Seedling that 1 leaf 1 saves or 2 leaves 2 save and is planted to collect seedling in garden, Strengthen rich water quality management, in time growing up to 11-15 leaf, take same method to continue expanding propagation, as planting transplantation of seedlings after growing up to potato Seedling To big field.
Collect seedling after the short Seedling of plantation grows up to strong sprout in garden, strong sprout can be cut short as several sections, plant in seed field, strengthen Field management, expanding propagation potato seed.
Potato Seedling is transplanted to what big field carried out breeding as seedling, carries out cuttage according to the equidistant 5-8cm of row strain.
The invention has the beneficial effects as follows: the invention provides a kind of detoxification business's potato 19 rapid propagation method, solve de- Poison business's potato 19 breed supply problem, achieve good economic benefit, effect of increasing production is obvious.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1:
A kind of detoxification business's potato 19 rapid propagation method, concretely comprises the following steps:
(1) without the acquisition of Solanum carolinense Seedling:
Business's potato 19 shoot tip meristem virus concentration is low, and have does not even contain virus, by band poison plant or tuber grown on top Point cuts, and carries out tissue culture, obtains without Solanum carolinense Seedling；
(2) selection in seedbed:
Select high-land, leeward on the sunny side, near water source, rich in organic matter, within 3 years, do not planted business's potato 19, and 600m around 19 kinds of chessons planted of band poison business potato are not as seedbed；The thin rake of deep ploughing before nursery, farm manure 4500kg, carbon are executed in every mu of seedbed Ammonium 30kg, makes the furrow face that width is 1.2m；
(3) selected potato seed:
Epidermis potato seed smooth, anosis, without wound, without freeze injury is selected to carry out seeding；With the water seed soaking of 55 DEG C before row's potato 13min, to kill potato block pathogenic bacteria；
(4) seeding:
The seeding time is early Febuary, is put by seedbed table mound before seeding, pads one layer of pig fully become thoroughly decomposed at the bottom of bed Cattle pen excrement, then repaves table soil thick for last layer 26cm；To distinguish end to end during seeding, it is impossible to the row of falling；Potato seed separates by size, flat Put dilute putting, the head of second piece of potato seed is pressed at the 1/3 of first piece of potato seed afterbody, the space of potato seed left and right Ge Liu 1cm；1m2 The flow rate in seedbed is 21kg；After drained kind, loam calking, water permeable, cover table soil thick for 2cm immediately, make potato seed not expose, Then tile mulch film, heat and moisture preserving；
(5) seedbed management:
Detoxification business's potato 19 nursery uses heating type hot house nursery on the ground, should heat in advance, make seedbed before potato seed discharge Temperature reaches more than 29 DEG C, and in nursery the 1st week, nursery bed temperature controls, at 36 DEG C, to be gradually decreased to 31 DEG C later, Seedling all sprout after temperature Control at 26 DEG C；Low temperature it is strictly on guard against, in order to avoid potato Seedling produces freeze injury during nursery；After potato bud is unearthed, throw off mulch film, it is to avoid burn seedlings；Newly After leaf starts to spread out, open thin film time when the fine day morning 10 to afternoon 3 and ventilate；Cutting 3d dry in the sun on daytime before Seedling, evening, epiphragma, logical Wind printing opacity seedling exercising；
(6) expanding propagation:
When seedbed potato Seedling length is to 11 leaf, potato Seedling is cut into the short Seedling that 1 leaf 1 saves and is planted to collect seedling in garden, strengthen rich water pipe Reason, in time growing up to 11 leaf, takes same method to continue expanding propagation, is transplanted to big field as seedling after growing up to potato Seedling.
Collect seedling after the short Seedling of plantation grows up to strong sprout in garden, strong sprout can be cut short as several sections, plant in seed field, strengthen Field management, expanding propagation potato seed.
Potato Seedling is transplanted to what big field carried out breeding as seedling, carries out cuttage according to the equidistant 5cm of row strain.
The invention provides a kind of detoxification business's potato 19 rapid propagation method, the supply of breeding solving detoxification business's potato 19 is asked Topic, achieves good economic benefit, and effect of increasing production is obvious.
Embodiment 2:
A kind of detoxification business's potato 19 rapid propagation method, concretely comprises the following steps:
(1) without the acquisition of Solanum carolinense Seedling:
Business's potato 19 shoot tip meristem virus concentration is low, and have does not even contain virus, by band poison plant or tuber grown on top Point cuts, and carries out tissue culture, obtains without Solanum carolinense Seedling；
(2) selection in seedbed:
Select high-land, leeward on the sunny side, near water source, rich in organic matter, within 5 years, do not planted business's potato 19, and 800m around 19 kinds of chessons planted of band poison business potato are not as seedbed；The thin rake of deep ploughing before nursery, farm manure 4800kg, carbon are executed in every mu of seedbed Ammonium 40kg, makes the furrow face that width is 1.2m；
(3) selected potato seed:
Epidermis potato seed smooth, anosis, without wound, without freeze injury is selected to carry out seeding；With 70% thiophanate methyl 500 before row's potato Times immersion kind 7min, to kill potato block pathogenic bacteria；
(4) seeding:
The seeding time is mid-February, is put by seedbed table mound before seeding, pads one layer of pig fully become thoroughly decomposed at the bottom of bed Cattle pen excrement, then repaves table soil thick for last layer 30cm；To distinguish end to end during seeding, it is impossible to the row of falling；Potato seed separates by size, flat Put dilute putting, the head of second piece of potato seed is pressed at the 1/3 of first piece of potato seed afterbody, the space of potato seed left and right Ge Liu 2cm；1m2 The flow rate in seedbed is 21kg；After drained kind, loam calking, water permeable, cover table soil thick for 3cm immediately, make potato seed not expose, Then tile mulch film, heat and moisture preserving；
(5) seedbed management:
Detoxification business's potato 19 nursery uses heating type hot house nursery on the ground, should heat in advance, make seedbed before potato seed discharge Temperature reaches more than 29 DEG C, and in nursery the 1st week, nursery bed temperature controls, at 36 DEG C, to be gradually decreased to 31 DEG C later, Seedling all sprout after temperature Control at 27 DEG C；Low temperature it is strictly on guard against, in order to avoid potato Seedling produces freeze injury during nursery；After potato bud is unearthed, throw off mulch film, it is to avoid burn seedlings；Newly After leaf starts to spread out, open thin film time when the fine day morning 10 to afternoon 3 and ventilate；Cutting 4d dry in the sun on daytime before Seedling, evening, epiphragma, logical Wind printing opacity seedling exercising；
(6) expanding propagation:
When seedbed potato Seedling length is to 15 leaf, potato Seedling is cut into the short Seedling that 2 leaves 2 save and is planted to collect seedling in garden, strengthen rich water pipe Reason, in time growing up to 15 leaf, takes same method to continue expanding propagation, is transplanted to big field as seedling after growing up to potato Seedling.
Collect seedling after the short Seedling of plantation grows up to strong sprout in garden, strong sprout can be cut short as several sections, plant in seed field, strengthen Field management, expanding propagation potato seed.
Potato Seedling is transplanted to what big field carried out breeding as seedling, carries out cuttage according to the equidistant 8cm of row strain.
The invention provides a kind of detoxification business's potato 19 rapid propagation method, the supply of breeding solving detoxification business's potato 19 is asked Topic, achieves good economic benefit, and effect of increasing production is obvious.
Embodiment 3:
A kind of detoxification business's potato 19 rapid propagation method, concretely comprises the following steps:
(1) without the acquisition of Solanum carolinense Seedling:
Business's potato 19 shoot tip meristem virus concentration is low, and have does not even contain virus, by band poison plant or tuber grown on top Point cuts, and carries out tissue culture, obtains without Solanum carolinense Seedling；
(2) selection in seedbed:
Select high-land, leeward on the sunny side, near water source, rich in organic matter, within 4 years, do not planted business's potato 19, and 700m around 19 kinds of chessons planted of band poison business potato are not as seedbed；The thin rake of deep ploughing before nursery, farm manure 4600kg, carbon are executed in every mu of seedbed Ammonium 35kg, makes the furrow face that width is 1.2m；
(3) selected potato seed:
Epidermis potato seed smooth, anosis, without wound, without freeze injury is selected to carry out seeding；With 70% thiophanate methyl 500 before row's potato Times immersion kind 8min, to kill potato block pathogenic bacteria；
(4) seeding:
The seeding time is mid-February, is put by seedbed table mound before seeding, pads one layer of pig fully become thoroughly decomposed at the bottom of bed Cattle pen excrement, then repaves table soil thick for last layer 28cm；To distinguish end to end during seeding, it is impossible to the row of falling；Potato seed separates by size, flat Put dilute putting, the head of second piece of potato seed is pressed at the 1/3 of first piece of potato seed afterbody, the space of potato seed left and right Ge Liu 2cm；1m2 The flow rate in seedbed is 21kg；After drained kind, loam calking, water permeable, cover table soil thick for 3cm immediately, make potato seed not expose, Then tile mulch film, heat and moisture preserving；
(5) seedbed management:
Detoxification business's potato 19 nursery uses heating type hot house nursery on the ground, should heat in advance, make seedbed before potato seed discharge Temperature reaches more than 29 DEG C, and in nursery the 1st week, nursery bed temperature controls, at 36 DEG C, to be gradually decreased to 31 DEG C later, Seedling all sprout after temperature Control at 27 DEG C；Low temperature it is strictly on guard against, in order to avoid potato Seedling produces freeze injury during nursery；After potato bud is unearthed, throw off mulch film, it is to avoid burn seedlings；Newly After leaf starts to spread out, open thin film time when the fine day morning 10 to afternoon 3 and ventilate；Cutting 3d dry in the sun on daytime before Seedling, evening, epiphragma, logical Wind printing opacity seedling exercising；
(6) expanding propagation:
When seedbed potato Seedling length is to 13 leaf, potato Seedling is cut into the short Seedling that 2 leaves 2 save and is planted to collect seedling in garden, strengthen rich water pipe Reason, in time growing up to 13 leaf, takes same method to continue expanding propagation, is transplanted to big field as seedling after growing up to potato Seedling.
Collect seedling after the short Seedling of plantation grows up to strong sprout in garden, strong sprout can be cut short as several sections, plant in seed field, strengthen Field management, expanding propagation potato seed.
Potato Seedling is transplanted to what big field carried out breeding as seedling, carries out cuttage according to the equidistant 7cm of row strain.
The invention provides a kind of detoxification business's potato 19 rapid propagation method, the supply of breeding solving detoxification business's potato 19 is asked Topic, achieves good economic benefit, and effect of increasing production is obvious.
Above the present invention is exemplarily described, it is clear that the present invention implements and is not subject to the restrictions described above, As long as have employed method design and the various improvement that carry out of technical scheme of the present invention, or the most improved directly apply to other Close, all within protection scope of the present invention.

Claims (3)

1. detoxification business's potato 19 rapid propagation method, it is characterised in that concretely comprise the following steps:
(1) without the acquisition of Solanum carolinense Seedling:
Business's potato 19 shoot tip meristem virus concentration is low, and have does not even contain virus, band poison plant or tuber grown on top point is cut Under, carry out tissue culture, obtain without Solanum carolinense Seedling；
(2) selection in seedbed:
Select high-land, leeward on the sunny side, near water source, rich in organic matter, 3-5 did not plant business's potato 19, and 600-around 800m not 19 kinds of chessons planted of band poison business potato are as seedbed；The thin rake of deep ploughing before nursery, farm manure 4500-is executed in every mu of seedbed 4800kg, carbon ammonium 30-40kg, make the furrow face that width is 1.2m；
(3) selected potato seed:
Epidermis potato seed smooth, anosis, without wound, without freeze injury is selected to carry out seeding；With the water seed soaking 13min of 55 DEG C before row's potato, Or by 500 times of immersion kinds 7-8min of 70% thiophanate methyl, to kill potato block pathogenic bacteria；
(4) seeding:
The seeding time is early Febuary or the middle ten days, is put by seedbed table mound before seeding, and at the bottom of bed, one layer of pad fully becomes thoroughly decomposed Pigs and cattle hurdle excrement, then repaves table soil thick for last layer 26-30cm；To distinguish end to end during seeding, it is impossible to the row of falling；Potato seed divides by size Opening, yaw is dilute puts, and is pressed in by the head of second piece of potato seed at the 1/3 of first piece of potato seed afterbody, the sky of potato seed left and right Ge Liu 1-2cm Gap；1m2The flow rate in seedbed is 21kg；After drained kind, loam calking, water permeable, cover table soil thick for 2-3cm immediately, make kind Potato does not exposes, and then tile mulch film, heat and moisture preserving；
(5) seedbed management:
Detoxification business's potato 19 nursery uses heating type hot house nursery on the ground, should heat in advance, make nursery bed temperature before potato seed discharge Reaching more than 29 DEG C, in nursery the 1st week, nursery bed temperature controls, at 36 DEG C, to be gradually decreased to 31 DEG C later, and after Seedling all sprouts, temperature controls At 26-27 DEG C；Low temperature it is strictly on guard against, in order to avoid potato Seedling produces freeze injury during nursery；After potato bud is unearthed, throw off mulch film, it is to avoid burn seedlings；Young leaves After starting to spread out, open thin film time when the fine day morning 10 to afternoon 3 and ventilate；Cutting 3-4d dry in the sun on daytime before Seedling, evening, epiphragma, logical Wind printing opacity seedling exercising；
(6) expanding propagation:
When seedbed potato Seedling length is to 11-15 leaf, potato Seedling is cut into the short Seedling that 1 leaf 1 saves or 2 leaves 2 save and is planted to collect seedling in garden, strengthen Rich water quality management, in time growing up to 11-15 leaf, takes same method to continue expanding propagation, is transplanted to greatly as seedling after growing up to potato Seedling Field.
A kind of detoxification business's potato 19 rapid propagation method the most according to claim 1, it is characterised in that plant in garden of collecting seedling Short Seedling grow up to strong sprout after, strong sprout can be cut short as several sections, plant in seed field, enhance field management, expanding propagation kind Potato.
A kind of detoxification business's potato 19 rapid propagation method the most according to claim 2, it is characterised in that potato Seedling is as seedling It is transplanted to what big field carried out breeding, carries out cuttage according to the equidistant 5-8cm of row strain.
CN201610632450.1A 2016-08-03 2016-08-03 A kind of detoxification business's potato 19 rapid propagation method Pending CN106165607A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106613190A (en) * 2016-12-02 2017-05-10 海南省农业科学院粮食作物研究所 Expanding propagation and planting method for ipomoea batatas virus-free seedlings
CN107242001A (en) * 2017-07-05 2017-10-13 海南省农业科学院粮食作物研究所 A kind of detoxification sweet potato potato seed method for culturing seedlings

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106613190A (en) * 2016-12-02 2017-05-10 海南省农业科学院粮食作物研究所 Expanding propagation and planting method for ipomoea batatas virus-free seedlings
CN107242001A (en) * 2017-07-05 2017-10-13 海南省农业科学院粮食作物研究所 A kind of detoxification sweet potato potato seed method for culturing seedlings

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Application publication date: 20161130