CN106134948A

CN106134948A - A kind of strawberry is with disease-resistant antibacterial cultivation matrix

Info

Publication number: CN106134948A
Application number: CN201610494487.2A
Authority: CN
Inventors: 张殿兴
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-06-28
Filing date: 2016-06-28
Publication date: 2016-11-23

Abstract

The invention discloses the disease-resistant antibacterial cultivation matrix of a kind of strawberry, be prepared by the raw materials in: aquatic products leftover bits and pieces 23 26, banana skin powder 69, potassium citrate 34, ferrous sulfate 45, glucolactone 23, calcium lime powder 68, sunflower plate granulated slag 36 40, snakeskin waste material 32 35, pepsin preparation 23,1 (3 dimethylamino-propyl) 3 ethyl-carbodiimide hydrochloride 1.3 1.6, N HOSu NHS 0.3 0.5,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate；The culture matrix of the present invention is by the interpolation of the organic material fully become thoroughly decomposed and calcium lime powder, ensure that in cultivation matrix and carry without pathogen, optimize the colony structure in matrix simultaneously, strengthen the breeding of probiotics, growing of antagonism pathogen, improve the micro-ecological environment of strawberry root system, play disease-resistant antibacterial purpose, for the matrix physicochemical environment of the high quality growth offer high-quality of strawberry.

Description

A kind of strawberry is with disease-resistant antibacterial cultivation matrix

Technical field

The present invention relates to nutrient matrix technical field, particularly relate to the disease-resistant antibacterial cultivation matrix of a kind of strawberry.

Background technology

Substrate culture is the current agriculture of China, woods, a kind of important cultural method in flower planting industry, is that one passes through base Matter absorbs a kind of Soilless Culture Methods of nutrient solution and oxygen, owing to it is to soil environment requirement is low, equipment simple, water-saving saves fertilizer etc. Advantage, is adopted application by vast plant development person.And continuous with the development of China's plant husbandry and cultivated area Expanding, the usage amount of cultivation matrix also constantly increases, and traditional uses the single raw material such as the peat composed of rotten mosses, vermiculite or be simply mixed preparation Matrix can not meet the demand of plantation, and the universal nutrition of present stage matrix simultaneously is single, causes sending out of plant in cultivation Bud rate is low, survival rate is low, the physical instability of matrix during simultaneously using, water imbibition difference, easily subside, surcharge Low, it is impossible to realize higher using value for specific environment, affect the growth of plantation economy benefit.

Collagen, as a kind of large biological molecule, has good pore structure after being modified, preferably raw Thing degradability, mechanical strength and biocompatibility, have good cell growth interface, can become a kind of cultivation matrix and build In quality raw materials.China snake aboundresources simultaneously, owing to the decorative pattern of snakeskin outward appearance is clearly gorgeous, pattern is unique, is used for making The handicraft of various finenesses, but due to the backwardness of snakeskin technology, process creates a large amount of waste material, account for complete The 20-30% of snakeskin, if not being effectively treated, not only polluting environment, also wasting substantial amounts of raw material, containing substantial amounts of in snakeskin Collagen, this also applies for it and provides cheap raw material basis in substrate preparation.

The application for a patent for invention of Publication No. CN1702061A discloses the full nutrient matrix of a kind of botanical seedling culturing and cultivation, This nutrient matrix use weight than for the peat of 50-70%, the perlite of 20-25%, the vermiculite of 10-15 and active material, Nutriment mixes, and has more comprehensive nutrients structure, but the cultivation matrix of this disclosure of the invention is due to particle size not One, easily cause sedimentation after watering, cause the blocking of air in matrix, the crude fiber content in matrix is low simultaneously, needs extra battalion Support the interpolation of material, improve production cost, and the real needs for cultivation do not improve the additional use value of matrix, limit Make the scale application of product.

Content of the invention

The object of the invention is contemplated to make up the defect of prior art, provides a kind of strawberry disease-resistant antibacterial cultivation base Matter.

The present invention is achieved by the following technical solutions:

A kind of strawberry with disease-resistant antibacterial cultivation matrix, is prepared by the raw materials in: aquatic products leftover bits and pieces 23-26, Banana skin powder 6-9, potassium citrate 3-4, ferrous sulfate 4-5, glucolactone 2-3, calcium lime powder 6-8, sunflower plate granulated slag 36-40, snakeskin waste material 32-35, pepsin preparation 2-3,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride 1.3-1.6, N-hydroxy-succinamide 0.3-0.5,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is appropriate, water In right amount.

Described strawberry is specifically comprised the following steps that by disease-resistant antibacterial cultivation matrix preparation method

(1) it is placed in aquatic products leftover bits and pieces, the mixing of banana skin powder in fermentation vat, add water to the 68-of ferment heap maximum water holding capacity 72%, ferment 18-20 days, ferment fermentation material squeeze and filter after completing, obtain zymotic fluid and ferment slag is standby, simultaneously by sunflower plate Cut off, pulverize as smalls standby；

(2) add 4% sodium chloride solution by 1:16-20g/mL solid-to-liquid ratio after snakeskin waste material being shredded, soak 8-10 hour After with 5800-6200 rev/min of centrifugal 12-15 minute, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with 0.15mol/L citric acid solution is mixed by 1:8-10g/mL solid-to-liquid ratio, adds pepsin preparation, is heated to 30-32 DEG C, 18-20 hour is extracted in insulation, is heated to 85-95 DEG C, is incubated 10 minutes after completing, then centrifuges with 5000-5500 rev/min 10-12 minute, collects supernatant liquor, obtains collagen solution standby；

(3) in step 1 gained zymotic fluid, add potassium citrate, ferrous sulfate, glucolactone, be heated to 54-56 DEG C, reaction 25-30 minute after cooling, then with step 1 gained ferment slag, sunflower plate granulated slag, calcium lime powder mixing for standby use；

(4) join step 2 gained collagen solution in step 3 gained material, be placed under 4-5 DEG C of low temperature environment, Stirring 5-10 minute after add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide with And other leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-35 DEG C of drying, obtains matrix of the present invention.

The invention have the advantage that

Matrix of the present invention is by the extraction to snakeskin, and by having reduced immunogenicity, the collagen of biodegradability should Use in the preparation of cultivation matrix, utilize the mechanical performance of cross-linking modified raising albumen, it is achieved its support in matrix structure Effect, can not only provide certain support and growth interface, the collagenous fiber bundle phase within collagen simultaneously for seed seedling Be staggered porous network structure mutually that formed, is also beneficial to the extension of beneficial microorganism lift-launch and root cell, and is It provides sufficient oxygen and nutriment, promotes the growth of plant, and plays fixation to culture matrix, convenient transport.

The culture matrix of the present invention is by the interpolation of the organic material fully become thoroughly decomposed and calcium lime powder, it is ensured that cultivation matrix Middle carry without pathogen, optimize the colony structure in matrix simultaneously, strengthen the breeding of probiotics, the taste of antagonism pathogen Raw, improve the micro-ecological environment of strawberry root system, play disease-resistant antibacterial purpose, for the base of the high quality growth offer high-quality of strawberry Matter physicochemical environment.

Detailed description of the invention

A kind of strawberry, with disease-resistant antibacterial cultivation matrix, is made up of the raw material of following weight portion (kg): aquatic products leftover bits and pieces is the 23rd, Banana skin powder the 6th, potassium citrate the 3rd, ferrous sulfate the 4th, glucolactone the 2nd, calcium lime powder the 6th, sunflower plate granulated slag the 36th, snakeskin waste material 32nd, pepsin preparation the 2nd, 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride the 1.3rd, N HOSu NHS 0.3rd, 4% sodium chloride solution is appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate.

(1) it is placed in aquatic products leftover bits and pieces, the mixing of banana skin powder in fermentation vat, add water to the 68% of ferment heap maximum water holding capacity, Ferment 18 days, fermentation complete after by fermentation material squeeze and filter, obtain zymotic fluid and ferment slag be standby, sunflower plate is cut off, powder simultaneously Broken standby for smalls；

(2) after snakeskin waste material being shredded by 1:16g/mL solid-to-liquid ratio add 4% sodium chloride solution, after soaking 8 hours with 5800 revs/min centrifuge 12 minutes, take precipitation clear water and clean and dry at 30 DEG C, then will precipitation and 0.15mol/L citric acid Solution is mixed by 1:8g/mL solid-to-liquid ratio, adds pepsin preparation, is heated to 30 DEG C, and insulation is extracted 18 hours, after completing It is heated to 85 DEG C, is incubated 10 minutes, then centrifuge 10 minutes with 5000 revs/min, collect supernatant liquor, obtain collagen solution Standby；

(3) in step 1 gained zymotic fluid, add potassium citrate, ferrous sulfate, glucolactone, be heated to 54 DEG C, After reacting 25 minutes cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, calcium lime powder mixing for standby use；

(4) join step 2 gained collagen solution in step 3 gained material, be placed under 4 DEG C of low temperature environments, stir After mixing 5 minutes add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N HOSu NHS and other Leftover materials, cross-linking reaction 8 hours, after completing, material is warming up to 30 DEG C of drying, obtains matrix of the present invention.

In order to further illustrate the using value of the present invention, inventor have chosen respectively matrix of the present invention and commercially available commonly Culture matrix carries out the cultivating and growing of strawberry, and during plantation, management method is identical, during plantation carries out the physical and chemical index of matrix Detection, result is as follows:

	Full nitrogen (%)	Full phosphorus (%)	Full potassium (%)	Total porosity (%)	Saturation moisture content (%)
						Matrix of the present invention	0.917	0.11	0.45	89.63	805
Commercially available generic media	0.728	0.08	0.41	84.83	413

Matrix simultaneously of the present invention plantation gained Yield of Strawberry improves more than 10% compared with generic media, and disease incidence reduces Within 2%, it can be seen that matrix of the present invention is nutritionally more sufficient compared with generic media, stable in physicochemical property simultaneously, structure Excellent, there is preferable prospect of the application.

Claims

1. a strawberry is with disease-resistant antibacterial cultivation matrix, it is characterised in that be prepared by the raw materials in: aquatic products is got a foothold Material 23-26, banana skin powder 6-9, potassium citrate 3-4, ferrous sulfate 4-5, glucolactone 2-3, calcium lime powder 6-8, Xiang Certain herbaceous plants with big flowers dish granulated slag 36-40, snakeskin waste material 32-35, pepsin preparation 2-3,1-(3-dimethylamino-propyl)-3-ethyl carbodiimide Hydrochloride 1.3-1.6, N-hydroxy-succinamide 0.3-0.5,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is fitted Amount, water are appropriate.

2. the strawberry according to claims 1 is with disease-resistant antibacterial cultivation matrix, it is characterised in that preparation method concrete Step is as follows:

(1) it is placed in aquatic products leftover bits and pieces, the mixing of banana skin powder in fermentation vat, add water to the 68-72% of ferment heap maximum water holding capacity, send out Ferment 18-20 days, fermentation complete after by fermentation material squeeze and filter, obtain zymotic fluid and ferment slag be standby, sunflower plate is cut off, powder simultaneously Broken standby for smalls；

(2) add 4% sodium chloride solution by 1:16-20g/mL solid-to-liquid ratio after snakeskin waste material being shredded, soak after 8-10 hour with 5800-6200 rev/min of centrifugal 12-15 minute, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with 0.15mol/L citric acid solution is mixed by 1:8-10g/mL solid-to-liquid ratio, adds pepsin preparation, is heated to 30-32 DEG C, 18-20 hour is extracted in insulation, is heated to 85-95 DEG C, is incubated 10 minutes after completing, then centrifuges with 5000-5500 rev/min 10-12 minute, collects supernatant liquor, obtains collagen solution standby；

(4) join step 2 gained collagen solution in step 3 gained material, be placed under 4-5 DEG C of low temperature environment, stirring After 5-10 minute add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide and its His leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-35 DEG C of drying, obtains matrix of the present invention.