CN106116716A - A kind of method utilizing Mulberry bar and bagasse to produce Pleurotus geesteranus - Google Patents
A kind of method utilizing Mulberry bar and bagasse to produce Pleurotus geesteranus Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
A kind of method utilizing Mulberry bar and bagasse to produce Pleurotus geesteranus, Guangxi resourceful Mulberry bar and bagasse is utilized to make the planting material of Pleurotus geesteranus, by using mixed vaccine that planting material is fermented, the rapid conversion of accelerated degradation raw material organic substance, promote organic matter degradation, make full use of Mulberry bar and the nutrient content of bagasse raising planting material, make Pleurotus geesteranus get bumper crops, improve Pleurotus geesteranus quality, increase the economic benefit of peasant.
Description
Technical field
The invention belongs to fungus growing technique, particularly to a kind of side utilizing Mulberry bar and bagasse to produce Pleurotus geesteranus
Method.
Background technology
Pleurotus geesteranus (Pleurotus geesteranus) belong on taxonomy Pleurotus geesteranus Eumycota, Basidiomycetes,
Agaricales, Pleurotaceae pleurotus.Title derives from TaiWan, China, and it is different from common Pleurotus sajor-caju and is because that it is less, and handle has 5
～6 centimetres, lid diameter < 3 centimetres.
Measure in Pleurotus geesteranus containing protein 3.65-3.88%, thick according to Fujian Academy of Agricultural Sciences motion soil and fertilizer institute
Fat 1.13-1.18%, reducing sugar 0.87-1.80%, sugar 23.94-34.87%, lignin 2.64%, cellulose 12.85%, really
Glue 0.14%, possibly together with cellulose, minerals etc..Visible, Pleurotus geesteranus is a kind of high protein, low-fat nutraceutical, and it is fresh
U.S. good to eat, there is the local flavor of uniqueness, call it by the fine-sounding name of " monosodium glutamate mushroom ".
Growing environment:
Temperature
The humidity range of mycelial growth is 10-35 DEG C, raw intensification plan 25-27 DEG C of optimum.Temperature is less than 10 DEG C, and mycelia stops substantially
Only growth；Less than 20 DEG C, mycelial growth is slow.Temperature is higher than 30 DEG C, mycelial growth, sparse, and color and luster turns yellow, it is easy to aging；Temperature
Degree is higher than 37 DEG C, and mycelia stops growing.The temperature range of sporophore growth is relatively wide, under the conditions of 10-32 DEG C can fruiting, this
It is the place different from other Pleurotus ostreatus.Former base is formed and mushroom flower bud growth optimal temperature is 15-20 DEG C, and temperature is less than 10 DEG C, very
Produce former base less.Less than 15 DEG C, sporophore growth is slow.Temperature is higher than 25 DEG C, and the growth of mushroom flower bud is fast, and ripe morning, cap is ripe
Shi Duocheng funnel-form.In the fruiting stage, if there being bigger day and night temperature to stimulate, former base easily produces.
Moisture content and humidity
The basic demand water content 65% of mycelial growth, is formed to entity ripe from former base, it is desirable to relative air humidity is
85-90%, when relative air humidity is less than 70%, former base produces few, and mushroom piece is easy dry to wither.When relative humidity is higher than 95%, sporophore is easy
Deliquescing is rotted.
Air and illumination
In the mycelium stage, one needs special aeration condition, the sporophore stage, then needs good aeration condition, if empty
In gas, CO2 concentration is higher than 0.1%, easily forms cap little, the misshapen mushroom of stem length.In the mycelium stage, it is not necessary to illumination, son is real
In the body stage, needing illumination, scattered light can induce former base to be formed and differentiation.Not having illumination, sporophore can not produce.Sporophore exists
Between 200-2000 lux illumination, growth is normal, and light is the darkest, is easily formed misshapen mushroom, too strong, and special direct light sporophore is easy
Withered.
PH value
The suitable meta-alkali of Pleurotus geesteranus ambient growth, therefore produce the pH value of the lime water regulation compost of upper conventional 3 5%,
Typically adjust to 7.2-----7.5.So can not only promote mycelial growth, and the impact of summer high temperature mycete can also be suppressed.
Because the unique nutritive value of Pleurotus geesteranus, it is the most all the most delicious and inexpensive on people's dining table
The dish that improves grade, because of its advantageous disease-resistant health-preserving function, allow Pleurotus geesteranus become very powerful and exceedingly arrogant good merchantable brand on food market.Elegant
Precious mushroom on food market one jin to sell for 11 yuan of economic worths striking.If deep processing can be carried out make its economy of various semi-finished product
Benefit is said double, can sell for about 20 fast money for one jin.If energy establishing in large scale, the another sharp weapon that farmer increases income will be become, because of
The cultivation technique of this Pleurotus geesteranus is increasingly paid close attention to by peasant household, under be classified as the part Patents retrieved:
Chinese patent 1, title: the method utilizing Ramulus Mori plantation Pleurotus geesteranus；Application number: 201510106934.8；The applying date:
2015-03-12；Applicant: Xiangzhou County Office of Science and Technology；Address: 545800 Xiangzhou County, Laibin City, Guangxi Zhuang Autonomous Region silver
As road 38；Summary: a kind of method utilizing Ramulus Mori plantation Pleurotus geesteranus, process, culture medium including Ramulus Mori are prepared, pack, gone out
Bacterium inoculation, mycelia culture, management of producing mushroom, gather, it is characterised in that: the material component proportioning of described plantation Pleurotus geesteranus is: Ramulus Mori
Bits 40%-80%, dispensing has Semen Gossypii core 0%-20%, wood flour 15%-25%, bran skin 10%-15%, pulverized limestone 2%.The present invention provides a kind of
Utilize the agricultural wastes Ramulus Mori bits method as primary raw material plantation Pleurotus geesteranus of kind of Sang Yangcan, solve the cultivation of current Pleurotus geesteranus
The problem that raw material is not concentrated and Ramulus Mori resource is turned waste into wealth, thus realize the optimization of resource, promote the industry of Pleurotus geesteranus
Change fast development.
Chinese patent 2, title: a kind of Pleurotus geesteranus cultural method；Application number: 201510009959.6；The applying date: 2015-
01-09；Applicant: Wu Jinfei；Address: 315500 (Yue Lin East Roads 28, Fenghua City, Ningbo City, Zhejiang Province disease prevention and control center
Number)；Summary: a kind of Pleurotus geesteranus cultural method, is the technology being applied to by mushroom stem in Pleurotus geesteranus cultivation.The present invention is with existing
The advantage that technology is compared is: one be mushroom stem soak material be full-natural nutritive liquid, containing the biological active matter of abundant species
Matter, soaks material with mushroom stem and planting material is mixed and stirred and carried out fermentation of banking up, and fermentation material has promotion effect to the growth of oyster mushroom pieces
Should, show as prosperous, the anti-different rate of mycelium growth vigor strong；Two is after the second tide mushroom is gathered, by mushroom stem leachate rejuvenation mycelia effect
Good, the formation of base former to Pleurotus geesteranus and the growth of sporophore have facilitation, and the biological transformation ratio of third and fourth tide mushroom is high；Three are
The problem overcoming life-time service chemosynthesis class fertilizer and cause Pleurotus geesteranus quality to decline, improves the quality of Pleurotus geesteranus, with
Time also improve yield, total biological efficiency than matched group improve more than 35%；Four are to leave out the fuel needed for high temperature sterilize, joint
The about energy, eliminates the drawback increasing CO2 emission in tradition Edible Fungi.
Chinese patent 3, title: the cultural method of a kind of Pleurotus geesteranus；Application number: 201410056476.7；The applying date:
2014-02-19；Applicant: Wei Chengsuo；Address: 547500 Hechi City Yao Autonomous County of Bama, Guangxi Zhuang Autonomous Region bar horses
The new road construction in town 368；Summary: the cultural method of a kind of Pleurotus geesteranus, step is as follows: (1) weighs required each by weight percentage
Component raw material, leaf of Caulis Sacchari sinensis, Urtica cannabina L. stalk, corn cob, Urtica cannabina L. seed, Testa oryzae, Semen Maydis powder, pulverized limestone, sugar, then by leaf of Caulis Sacchari sinensis, Urtica cannabina L.
Bar, corn cob and Urtica cannabina L. seed mix with Testa oryzae, Semen Maydis powder after pulverizing respectively and pile bank, fermentation；(2) by sugar and Calx
Powder is soluble in water, stirs；(3) material after fermentation is mixed pack with material in (2)；(4) vexed 6～8h are continued after sterilizing
After make bacterium cylinder；(5) it is to cultivate 28～32d in 24～28 DEG C of light protected environment to mycelia maturation that bacterium cylinder is put into temperature；(6) will become
Ripe mycelia bacterium cylinder moves on to the fruiting field that relative air humidity is 80%～90% and cultivates, and gathers after 9～11d.It is contemplated that improve
The yield of Pleurotus geesteranus and quality thereof so that Pleurotus geesteranus more nutritive value, meanwhile, can use waste agronomic crop to carry out Pleurotus geesteranus
Cultivation so that cultural method environmental protection, low cost.
The method utilizing Mulberry bar to produce Pleurotus geesteranus is mentioned from above-mentioned document it can be seen that in patent 1, but in patent 1
Only being just used for cultivating Pleurotus geesteranus by after Mulberry bar simple process, on the one hand Mulberry bar itself contains a large amount of crude fibres, without processing
Mulberry bar nutrient content is the lowest, and the Pleurotus geesteranus quality of production is relatively low.
In addition, yet there are no utilize Mulberry bar and bagasse as main material production Pleurotus geesteranus method and
Technology, Mulberry bar and bagasse are all the agricultural residues that Guangxi is common, are typically only capable to take to burn or landfill disposal, have both caused money
Source waste also produces large quantity of exhaust gas contaminated air, how to utilize cheap Mulberry bar and bagasse to produce Pleurotus geesteranus and has high warp
Ji is worth.
Summary of the invention
It is an object of the invention to for problem above, utilize Guangxi Mulberry bar more typically and bagasse to make the cultivation of Pleurotus geesteranus
Training material, by using mixed vaccine that planting material is fermented, the rapid conversion of accelerated degradation raw material organic substance, promote Organic substance
Degraded, makes full use of the crude fibre in Mulberry bar and bagasse, improves the nutrient content of planting material, makes Pleurotus geesteranus get bumper crops:
Technical scheme is as follows:
Utilizing the method that Mulberry bar and bagasse produce Pleurotus geesteranus, its process is as follows:
(1) prepared by planting material: raw material includes the composition of following portions by weight:
Mulberry bar 40-60 part,
Bagasse 10-15 part,
Testa Tritici 3-5 part,
Semen Maydis powder 5-8 part,
Described Mulberry bar and bagasse break into powder before using, after the mixing of above-mentioned each composition, add planting material gross weight 3%-5%
Mixed fermentation 3-7 days, the planting material after fermentation with the vapour cooking 7 hours of 120 DEG C, is dried after disinfection and softening, dry
The packed bag of planting material polypropylene plastics, and bag mouth is tied rear standby；
(2) strain improvement: being accessed in culture medium by mother bacterium and cultivate, the original seed bacterium obtained after cultivation is accessed by culture medium and cultivation again
Expect that being enlarged cultivation in the mixed culture medium according to parts by weight 1:1 mixing obtains cultigen；
(3) send out bacterium to cultivate: putting into mushroom house after being linked in planting material by cultigen and cultivate, mushroom house temperature controls at 25-35
℃；Humid control, at 70%-80%, is aerated turning in every 3-4 hour, prevents high temperature from burning bacterium；
(4) management of producing mushroom: send out and carry out hanging fruiting after bacterium is cultivated 20-30 days, bag mouth is tightened and does not interspace, bacterium bag after tying
Being hung on the suspension bracket of mushroom house, and make upper and lower bag stagger, dosing open after hanging, opening is V-shaped, opens 10-15 mouth, treats for every bag
After Pleurotus geesteranus fruiting, timing every day is to opening surface spraying, keeps mushroom flower bud to moisten, gathers after mushroom producing culture 30-40 days.
(5) mushroom is gathered: gather after mushroom producing culture 30-40 days, can gather continuously 4 months.
Described culture medium prescription is as follows:
Rhizoma Solani tuber osi 70-80 part,
Glucose 10-20 part,
Peptone 10-20 part,
Agar powder 5-10 part,
Water 80-100 part,
Containing molasses in the potato culture that the present invention uses, molasses are the side-products of sugar industry, form because of sugaring raw material,
The difference of processing conditions and variant, wherein mainly contain a large amount of fermentable sugars (mainly sucrose), thus be well fermentation
Raw material, can be used as substrate or the base material of the fermented products such as yeast, monosodium glutamate, organic acid, and molasses contain a small amount of thick protein, generally
3% ~ 6%, belong to non-protein nitrogen class more, such as ammonia, amide and nitrate etc., and amino-acid nitrogen only accounts for 38% ~ 50%, and nonessential ammonia
Base acid is as more in aspartic acid, content of glutamic acid, it is possible to accelerate the formation of bacterium colony, and potato culture preparation is simple, energy
Enough mixing mutually with described planting material, applicable strain selects excellent, and mixing, by culture medium and planting material, the cultigen selected can
Adapt to planting material very well, fully absorb the nutrient utilizing planting material.
The present invention includes bacillus subtilis and EM bacterium, wherein for the mixed vaccine of planting material of fermenting:
Bacillus subtilis (Bacillus subtilis), is the one of Bacillus.Individual cells 0.7～0.8 × 2～3
Micron, uniform coloring.Without pod membrane, peritrichous, can move.Gram positive bacteria, spore 0.6～0.9 × 1.0～1.5 micron,
Oval to column, it is positioned at thalline central or slightly inclined, after sporulation, thalline does not expands.Bacterium colony rough surface is opaque, dirty white
Or slightly yellow, in liquid medium within during growth, it is commonly formed wrinkle mould.Aerobe.Available protein, multiple sugar and starch, point
Solving tryptophan and form indole, the present invention utilizes bacillus subtilis to decompose the crude fibre in bagasse and Mulberry bar, forms Pleurotus geesteranus
Albumen needed for sending out bacterium and growing up and nutrient.
EM bacterium (Effective Microorganisms) is made up of about 80 kinds of microorganisms, and EM bacterium is big by Ryukyu of Japan
The ratio learned good photograph husband taught for 1982 [1] years and studies successfully, put goods on the market the eighties.EM bacterium is with photosynthetic bacteria, lactic acid bacteria, ferment
The micro-life bacteria preparation of one that more than 80 microorganisms of 10 genus that female bacterium and actinomycetes are main are composited.The mechanism of action is shape
Become EM bacterium and the competition of pathogenic microorganism contention nutrition, due to em bacterium easily survival and reproduction in soil, so can be very fast and steady
Surely occupy the ecologic niche in soil, form the advantage group of useful microbial bacteria, thus control the numerous of pathogenic microorganism
Grow and invasion and attack to crop.Being the developing direction of the ecological agriculture, be more beneficial for the sustainable development of agricultural, the present invention utilizes EM bacterium
Solve the problem that in sweat, planting material is easily polluted by harmful bacteria, it is ensured that Pleurotus geesteranus is grown up healthy and sound.
Planting material raw material used by the present invention is mainly Mulberry bar and bagasse.Mulberry bar is one of agricultural residue, supports Bombyxmori Linnaeus
Growing district, annual peasants grow up strong and sturdy for Folium Mori, and the summer to be carried out is cut down and cuts down with the autumn, and the Mulberry bar cutting down out does not has value,
General peasant selects burning disposal, and undressed Mulberry bar contains 30% ~ 45% Organic substance, 50% ~ 65% crude fibre, by fermentation after can
To obtain cultivating the nutritional labeling needed for Pleurotus geesteranus；Bagasse is also one of agricultural residue, is that produce after sugar useless is squeezed by cane mill
Material, containing a large amount of celluloses in bagasse, it is also possible to the nutrient needed for providing Pleurotus geesteranus to grow up, undressed bagasse is general
Take to burn or landfill disposal.
The present invention significantly provides the benefit that:
(1) utilize and be both the Mulberry bar of agricultural residue and bagasse as the primary raw material of planting material, can turn waste into wealth, can fill again
Divide and utilize a large amount of crude fibres contained in Mulberry bar and bagasse, by adding mixed fermentation planting material, decompose Mulberry bar and Caulis Sacchari sinensis
Crude fibre in slag forms the nutritional labeling of crude protein one class, promotes organic matter degradation, improves raw material availability.The present invention also exists
Planting material is added with Testa Tritici and Semen Maydis powder, it is possible to shorten Pleurotus geesteranus and send out the time of bacterium and fruiting；Prevent Pleurotus geesteranus generation disease,
Make in Pleurotus geesteranus cultivation without spraying insect protected diseases prevention medicament again；Having only to 55-70 days from being inoculated into gather, cultivation cost is relatively
Low, hence it is evident that to increase economic benefit.
(2) Pleurotus geesteranus growth promoter needs carbon element, nitrogen and various mineral nutrients etc..It is S mushroom, with it
Its fungus is the same, without chlorophyll, it is impossible to oneself carries out photosynthesis and manufactures nutrient, and fully relies on ready-made organic substance battalion
Life.In its growth course, required carbon element, nitrogen, mineral and auxin etc., all obtain in planting material, warp
Cross series of process and carry out selection-breeding so that it is can adapt to planting material and local weather that the present invention selects, thus obtain preferably
Pleurotus geesteranus kind so that it is can preferably grow up.
Detailed description of the invention
Embodiment 1:
Utilizing the method that Mulberry bar and bagasse produce Pleurotus geesteranus, its production process is as follows:
(1) prepared by planting material: raw material includes the composition of following portions by weight:
40 parts of Mulberry bar,
Bagasse 10 parts,
3 parts of Testa Tritici,
Semen Maydis powder 5 parts,
Described Mulberry bar and bagasse break into powder before using, after the mixing of above-mentioned each composition, add planting material gross weight 3%-5%
Mixed fermentation 3-7 days, the planting material after fermentation with the vapour cooking 7 hours of 120 DEG C, is dried after disinfection and softening, dry
The packed bag of planting material polypropylene plastics, and bag mouth is tied rear standby；
(2) strain improvement: being accessed in culture medium by mother bacterium and cultivate, the original seed bacterium obtained after cultivation is accessed by culture medium and cultivation again
Expect that being enlarged cultivation in the mixed culture medium according to parts by weight 1:1 mixing obtains cultigen.
(3) send out bacterium to cultivate: putting into mushroom house after being linked in planting material by cultigen and cultivate, mushroom house temperature controls 25
℃；Humid control, 70%, is aerated turning in every 3 hours, prevents high temperature from burning bacterium.
(4) management of producing mushroom: send out and carry out hanging fruiting after bacterium is cultivated 10 days, bag mouth is tightened and does not interspace, bacterium after tying
Bag is hung on the suspension bracket of mushroom house, and makes upper and lower bag stagger, and dosing open after hanging, opening is V-shaped, opens 10 mouthfuls, treats for every bag
After Pleurotus geesteranus fruiting, timing every day is to opening surface spraying, keeps mushroom flower bud to moisten.
(5) mushroom is gathered: mushroom producing culture was gathered after 30 days, can gather continuously 4 months.
Described culture medium is potato culture, and formula is as follows:
Rhizoma Solani tuber osi 70 parts,
Glucose 10 parts,
Peptone 10 parts,
Agar powder 5 parts,
80 parts of water,
Mixed vaccine for planting material of fermenting includes bacillus subtilis, Trichoderma viride and EM bacterium, according to ratio of weight and number 1:1:1
Mixing.
Embodiment 2:
Utilizing the method that Mulberry bar and bagasse produce Pleurotus geesteranus, its production process is as follows:
(1) prepared by planting material: raw material includes the composition of following portions by weight:
50 parts of Mulberry bar,
Bagasse 12 parts,
4 parts of Testa Tritici,
Semen Maydis powder 6 parts,
Described Mulberry bar and bagasse break into powder before using, after the mixing of above-mentioned each composition, add planting material gross weight 3%-5%
Mixed fermentation 3-7 days, the planting material after fermentation with the vapour cooking 7 hours of 120 DEG C, is dried after disinfection and softening, dry
The packed bag of planting material polypropylene plastics, and bag mouth is tied rear standby；
(2) strain improvement: being accessed in culture medium by mother bacterium and cultivate, the original seed bacterium obtained after cultivation is accessed by culture medium and cultivation again
Expect that being enlarged cultivation in the mixed culture medium according to parts by weight 1:1 mixing obtains cultigen.
(3) send out bacterium to cultivate: putting into mushroom house after being linked in planting material by cultigen and cultivate, mushroom house temperature controls 25
℃；Humid control, 75%, is aerated turning in every 3 hours, prevents high temperature from burning bacterium.
(4) management of producing mushroom: send out and carry out hanging fruiting after bacterium is cultivated 10 days, bag mouth is tightened and does not interspace, bacterium after tying
Bag is hung on the suspension bracket of mushroom house, and makes upper and lower bag stagger, and dosing open after hanging, opening is V-shaped, opens 10 mouthfuls, treats for every bag
After Pleurotus geesteranus fruiting, timing every day is to opening surface spraying, keeps mushroom flower bud to moisten.
(5) mushroom is gathered: mushroom producing culture was gathered after 35 days, can gather continuously 4 months.
Described culture medium prescription is as follows:
Rhizoma Solani tuber osi 75 parts,
Glucose 15 parts,
Peptone 15 parts,
Agar powder 8 parts,
90 parts of water,
Mixed vaccine for planting material of fermenting includes bacillus subtilis, Trichoderma viride and EM bacterium, according to ratio of weight and number 1:1:1
Mixing.
Embodiment 3:
Utilizing the method that Mulberry bar and bagasse produce Pleurotus geesteranus, its production process is as follows:
(1) prepared by planting material: raw material includes the composition of following portions by weight:
60 parts of Mulberry bar,
Bagasse 15 parts,
5 parts of Testa Tritici,
Semen Maydis powder 8 parts,
Described Mulberry bar and bagasse break into powder before using, after the mixing of above-mentioned each composition, add planting material gross weight 3%-5%
Mixed fermentation 3-7 days, the planting material after fermentation with the vapour cooking 7 hours of 120 DEG C, is dried after disinfection and softening, dry
The packed bag of planting material polypropylene plastics, and bag mouth is tied rear standby；
(2) strain improvement: being accessed in culture medium by mother bacterium and cultivate, the original seed bacterium obtained after cultivation is accessed by culture medium and cultivation again
Expect that being enlarged cultivation in the mixed culture medium according to parts by weight 1:1 mixing obtains cultigen.
(3) send out bacterium to cultivate: putting into mushroom house after being linked in planting material by cultigen and cultivate, mushroom house temperature controls 28
℃；Humid control, 80%, is aerated turning in every 4 hours, prevents high temperature from burning bacterium.
(4) management of producing mushroom: send out and carry out hanging fruiting after bacterium is cultivated 15 days, bag mouth is tightened and does not interspace, bacterium after tying
Bag is hung on the suspension bracket of mushroom house, and makes upper and lower bag stagger, and dosing open after hanging, opening is V-shaped, opens 15 mouthfuls, treats for every bag
After Pleurotus geesteranus fruiting, timing every day is to opening surface spraying, keeps mushroom flower bud to moisten.
(5) mushroom is gathered: mushroom producing culture was gathered after 40 days, can gather continuously 4 months.
Described culture medium is potato culture, and formula is as follows:
Rhizoma Solani tuber osi 80 parts,
Glucose 20 parts,
Peptone 20 parts,
Agar powder 10 parts,
100 parts of water,
Mixed vaccine for planting material of fermenting includes bacillus subtilis, Trichoderma viride and EM bacterium, according to ratio of weight and number 1:1:1
Mixing.
The following is the present invention produce Pleurotus geesteranus method with other produce Pleurotus geesteranus method comparison:
| Pleurotus geesteranus growing state | Planting time | Collecting time continuously | Economic benefit | |
| Natural cultural method | Poor growth, the easily rotten mushroom of appearance | More than 90 days | 2-3 month | Cost is relatively low, income is low |
| The method making planting material with Semen Gossypii | Growth is very fast, fruiting amount is higher | 60-80 days | 2-3 month | Relatively costly, income is relatively low |
| Cultural method of the present invention | Growth is rapid, fruiting amount mushroom big, fresh is soft | 50-70 days | 4 months | Cost is relatively low, income is high |
Claims (3)
1. one kind utilizes the method that Mulberry bar and bagasse produce Pleurotus geesteranus, it is characterised in that its production process is as follows:
Prepared by planting material: choose the raw material of following portions by weight:
Mulberry bar 40-60 part,
Bagasse 10-15 part,
Testa Tritici 3-5 part,
Semen Maydis powder 5-8 part,
Described Mulberry bar and bagasse break into powder before using, after the mixing of above-mentioned each composition, add planting material gross weight 3%-5%
Mixed fermentation 3-7 days, the planting material after fermentation with the vapour cooking 7 hours of 120 DEG C, is dried after disinfection and softening, dry
The packed bag of planting material polypropylene plastics, and bag mouth is tied rear standby；
(2) strain improvement: being accessed in culture medium by mother bacterium and cultivate, the original seed bacterium obtained after cultivation is accessed by culture medium and cultivation again
Expect that being enlarged cultivation in the mixed culture medium according to parts by weight 1:1 mixing obtains cultigen；
(3) send out bacterium to cultivate: putting into mushroom house after being linked in planting material by cultigen and cultivate, mushroom house temperature controls at 25-35
℃；Humid control is at 70%-80%；
(4) management of producing mushroom: send out and carry out hanging fruiting after bacterium is cultivated 10-15 days, bag mouth is tightened and does not interspace, bacterium bag after tying
Being hung on the suspension bracket of mushroom house, and make upper and lower bag stagger, dosing open after hanging, opening is V-shaped, opens 10-15 mouth, treats for every bag
After Pleurotus geesteranus fruiting, timing every day is to opening surface spraying, keeps mushroom flower bud to moisten；
(5) mushroom is gathered: gather after mushroom producing culture 30-40 days, can gather continuously 4 months.
The method utilizing Mulberry bar and bagasse to produce Pleurotus geesteranus the most according to claim 1, it is characterised in that: described training
Support based formulas as follows:
Rhizoma Solani tuber osi 70-80 part,
Glucose 10-20 part,
Peptone 10-20 part,
Agar powder 5-10 part,
Water 80-100 part.
The method utilizing Mulberry bar and bagasse to produce Pleurotus geesteranus the most according to claim 1, it is characterised in that: the present invention uses
Mixed vaccine in fermentation planting material includes bacillus subtilis, Trichoderma viride and EM bacterium, mixes according to ratio of weight and number 1:1:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610461789.XA CN106116716A (en) | 2016-06-23 | 2016-06-23 | A kind of method utilizing Mulberry bar and bagasse to produce Pleurotus geesteranus |
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| CN107950297A (en) * | 2017-12-11 | 2018-04-24 | 温立超 | A kind of method using Pleurotus eryngii bacteria residue cultivation elegant precious mushroom |
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| CN103416224A (en) * | 2013-08-08 | 2013-12-04 | 刘万顺 | Method for preparing pleurotus geesteranus compost |
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| CN107950297A (en) * | 2017-12-11 | 2018-04-24 | 温立超 | A kind of method using Pleurotus eryngii bacteria residue cultivation elegant precious mushroom |
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