CN106069901A - A kind of quickly discriminating mandarin sturgeon property method for distinguishing and used tool - Google Patents
A kind of quickly discriminating mandarin sturgeon property method for distinguishing and used tool Download PDFInfo
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- CN106069901A CN106069901A CN201610431873.7A CN201610431873A CN106069901A CN 106069901 A CN106069901 A CN 106069901A CN 201610431873 A CN201610431873 A CN 201610431873A CN 106069901 A CN106069901 A CN 106069901A
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- mandarin sturgeon
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
A kind of quickly discriminating mandarin sturgeon property method for distinguishing, the method comprises the following steps: step 1: supporting temporarily of mandarin sturgeon to be identified prepares；Step 2: configuration anesthesia liquid；Step 3: the anesthesia of mandarin sturgeon to be identified；Step 4: abdominal incision；Step 5: sex abnormality；Step 6: wound processes；Step 7: the recovery after discriminating and recovery；I.e. complete the quick discriminating of mandarin sturgeon sex.A kind of quickly discriminating mandarin sturgeon property method for distinguishing and the used tool that the present invention provides, can solve to lack the problem of mandarin sturgeon sexual maturity sex discriminating method in early days, can differentiate mandarin sturgeon sex quickly, efficiently, accurately；Easy to operate, practicality is extremely strong, meets the demand of sex abnormality in the work such as mandarin sturgeon aquaculture, scientific research.
Description
Technical field
The present invention relates to aquaculture field, especially a kind of quickly discriminating mandarin sturgeon property method for distinguishing and used tool.
Background technology
Mandarin sturgeon is a kind of large-scale anadromous migration Fish, be in the existing Fish in the world the most rare the most original kind it
One, mandarin sturgeon Ye Shi China one-level lays special stress on protecting wild animal, enjoys the good reputation of " giant panda in water ".During the sexual maturity of mandarin sturgeon
Between longer, the female mandarin sturgeon for the first time sexually matured time is generally 14～26 years (average 18 years), and male mandarin sturgeon is for the first time
The sexually matured time is generally 8～18 years (average 12 years).In the breeding process of mandarin sturgeon, female owing to lacking effective discriminating
Male method, all of fish was all raised together with together before reaching age at sexual maturity；Only when proximity maturation age, gonad
Male and female could be distinguished by the technology such as B ultrasonic, puncture when feature is obvious.When utilizing B ultrasonic technology to differentiate mandarin sturgeon adult fish gonad, right
Adult fish gonad development require higher, only when in B ultrasonic image male and female gonad feature clearly in the case of could effectively enter
Row differentiates, and the detectability of the method is relatively low, requires higher to the technology of operator；China is carried out utilizing the method punctured
During sturgeon sex abnormality, it is desirable to when the content feature of puncture acquirement is obvious, (female content typically has obvious graininess ovum grain；
The general white bulk of male content or refining) just can accurately carry out Sex judging.These methods cannot be accurately to low
Age, mandarin sturgeon carried out Sex judging, was unfavorable for that the group structure optimization of mandarin sturgeon artificial cultivation colony and the specific aim of parent fish are cultivated
And species conservation work carry out.
Summary of the invention
The technical problem to be solved is to provide a kind of quickly discriminating mandarin sturgeon property method for distinguishing and used tool,
Can solve to lack the problem of mandarin sturgeon sexual maturity sex discriminating method in early days, the mandarin sturgeon to sexual maturity (more than 6 ages) in early days
Sex abnormality can be carried out, overcome in existing method after only waiting mandarin sturgeon entering maturation that (average 18 years of raun, milter is put down
Equal 12 years) just can carry out the defect of sex abnormality, quickly, efficiently, accurately；Easy to operate, practicality is extremely strong, meet mandarin sturgeon increase
The demand of the sex abnormality in the work such as cultivation, scientific research, while guaranteeing high-accuracy, the injury to fish is less, is in discriminating
One of effective way of China's sturgeon sex.
For solving above-mentioned technical problem, the technical solution adopted in the present invention is: a kind of quick discriminating mandarin sturgeon sex
Method, the method comprises the following steps:
Step 1: supporting temporarily of mandarin sturgeon to be identified prepares: is put in storage pond, holding pond by mandarin sturgeon to be identified and supports temporarily, temporarily
The foster time is 2～3 days, supports period temporarily and stops eating, and ensures the most foster good water water quality；
Step 2: configuration anesthesia liquid；
Step 3: the anesthesia of mandarin sturgeon to be identified: mandarin sturgeon to be identified is put into anesthesia case and carries out anaesthetizing until fish body enters
Enter deep anaesthesia state；
Step 4: abdominal incision: take out mandarin sturgeon to be identified, between the pectoral fin and abdomeinal fin of mandarin sturgeon to be identified slightly toward
The side of pectoral fin, along abdomen hone lamella upper limb at the opening that side oddi's sphincteroplasty is 3～4 centimetres；
Step 5: sex abnormality: utilize special instrument of dehiscing, struts the upper opening cut of mandarin sturgeon to be identified, observes in it
The shape of portion's gonad, carries out sex abnormality according to the external appearance characteristic of male and female gonad；
Step 6: wound processes: sews up the wound of the mandarin sturgeon completing sex abnormality, and carries out anti-inflammatory treatment；
Step 7: the recovery after discriminating and recovery: by step 6 through treatment after mandarin sturgeon flowing water culture pond immediately in enter
Row recovery, and note following the tracks of the recovery situation observing wound in the cultivation in later stage, can be recovered treatment if desired；
I.e. complete the quick discriminating of mandarin sturgeon sex.
The water quality index supporting water in step 1 temporarily is: DO ＞ 6.0mg/L, NH4 +＜ 0.5mg/L, NO2 -＜ 0.05mg/L, pH
Value 7.5～8.5.
In step 1, the age of a fish >=6 year of mandarin sturgeon to be identified.
In step 2, the anesthesia liquid of preparation be concentration be the Oleum Caryophylli of 80～120ppm or MS-that concentration is 80～100ppm
222。
In step 4, the aperture position of incision is between pectoral fin and the abdomeinal fin of mandarin sturgeon, and center line between pectoral fin and abdomeinal fin
Distance d is 5～15 centimetres.
The discriminating operating process of step 5 controlled within the Anesthetic safety time of 5 minutes.
The anti-inflammatory treatment method of step 6 is: smears the mixture of erythromycin ointment and YUNNAN BAIYAO at wound, notes simultaneously
Penetrating the florfenicol injection that mass content is 5%, injection dosage is 0.2～0.5ml/kg body weight.
A kind of employing said method carries out mandarin sturgeon sex identification used tool, including first handle and second handle, the
The middle part of one handle and second handle is flexibly connected by bolt, the top of first handle and second handle be flared out with
Vertical being respectively fixedly connected with to the top of a in a certain angle, first handle and second handle supports mouth sheet, support mouth sheet and the company of handle
The place of connecing is positioned at support mouth sheet middle side edge.
The angle of angle a is 15～20 degree.
Support mouth sheet is the square lamellar body of a size of length of side 2 centimetres.
A kind of quickly discriminating mandarin sturgeon property method for distinguishing and the used tool that the present invention provides, proposes one quickly first
Differentiate mandarin sturgeon property method for distinguishing and specific purpose tool, can solve to lack asking of mandarin sturgeon sexual maturity sex discriminating method in early days
Topic, can carry out sex abnormality to the mandarin sturgeon of sexual maturity (more than 6 ages) in early days, overcomes and only waits mandarin sturgeon in existing method
After entering maturation, (average 18 years of raun, average 12 years of milter) just can carry out the defect of sex abnormality, quickly, efficiently, accurately；
Easy to operate, practicality is extremely strong, meet the demand of sex abnormality in the work such as mandarin sturgeon aquaculture, scientific research, guaranteeing Gao Zhun
Really less to the injury of fish while rate, it is one of effective way differentiating mandarin sturgeon sex.
By 36 tail mandarin sturgeons use the method for the present invention and instrument carry out sex identification, identify male and female mandarin sturgeon altogether
15 tails, male mandarin sturgeon 20 tail, gonad feature is inconspicuous, it is impossible to mandarin sturgeon 1 tail of differentiation, uses this method discriminating to male and female
Rate is 97%.
Accompanying drawing explanation
The invention will be further described with embodiment below in conjunction with the accompanying drawings:
Fig. 1 is the inventive method step 4 schematic diagram at the opening of mandarin sturgeon abdominal incision；
Fig. 2 is the schematic diagram of the external appearance characteristic of the male and female gonad that the inventive method step 5 is observed；
Fig. 3 is the structural representation of used tool of the present invention, and now two support mouth sheets are in closure state；
Fig. 4 is the structural representation of used tool of the present invention, and now two support mouth sheets are in open configuration；
Fig. 5 is the block diagram of used tool of the present invention.
Detailed description of the invention
Embodiment one
A kind of quickly discriminating mandarin sturgeon property method for distinguishing, the method comprises the following steps:
Step 1: supporting temporarily of mandarin sturgeon to be identified prepares: is put in storage pond, holding pond by mandarin sturgeon to be identified and supports temporarily, temporarily
The foster time is 2～3 days, supports period temporarily and stops eating, and ensures the most foster good water water quality；
Step 2: configuration anesthesia liquid；
Step 3: the anesthesia of mandarin sturgeon to be identified: mandarin sturgeon to be identified is put into anesthesia case and carries out anaesthetizing until fish body enters
Enter deep anaesthesia state (deep anaesthesia state can be entered in about 5～8 minutes),
Anesthesia case specification is 4 meters × 2 meters × 1 meter, and total measurement (volume) is 8 cubic metres, puts into 0.5 meter of deep water in anesthesia case,
The water yield is 48 cubic metres；
Step 4: abdominal incision: take out mandarin sturgeon to be identified, slightly biased between the pectoral fin 2 and abdomeinal fin 3 of mandarin sturgeon to be identified
To the side of pectoral fin, along abdomen hone lamella upper limb at the opening 1 that side oddi's sphincteroplasty is 3～4 centimetres, as shown in Figure 1；
Step 5: sex abnormality: utilize special instrument of dehiscing, struts the upper opening 1 cut of mandarin sturgeon to be identified, observes it
The shape of internal gonad, carries out sex abnormality according to the external appearance characteristic of male and female gonad, as in figure 2 it is shown,
Female gonads feature: gonad is that spire is branched, has the color characteristics such as yellowish, pink；
Male gonad feature: gonad is divided into some fritters, surface is smooth shape, has the color characteristic such as pale pink, white；
Step 6: wound processes: sews up the wound of the mandarin sturgeon completing sex abnormality, and carries out anti-inflammatory treatment；
Step 7: the recovery after discriminating and recovery: by step 6 through treatment after mandarin sturgeon flowing water culture pond immediately in enter
Row recovery, and note following the tracks of the recovery situation observing wound in the cultivation in later stage, can be recovered treatment if desired；
I.e. complete the quick discriminating of mandarin sturgeon sex.
The water quality index supporting water in step 1 temporarily is: DO ＞ 6.0mg/L, NH4 +＜ 0.5mg/L, NO2 -＜ 0.05mg/L, pH
Value 7.5～8.5, water temperature 15～16 DEG C.
In step 1, the age of a fish >=6 year of mandarin sturgeon to be identified.
In step 2, the anesthesia liquid of preparation be concentration be the Oleum Caryophylli of 80～120ppm or MS-that concentration is 80～100ppm
222。
In step 4, opening 1 position of incision is between the pectoral fin 2 of mandarin sturgeon and abdomeinal fin 3, and between pectoral fin 2 and abdomeinal fin 3
Distance d of center line 6 is 5～15 centimetres.
The discriminating operating process of step 5 controlled within the Anesthetic safety time of 5 minutes.
The anti-inflammatory treatment method of step 6 is: smear the mixture (erythromycin of erythromycin ointment and YUNNAN BAIYAO at wound
The mass ratio of ointment and YUNNAN BAIYAO is 3:1), simultaneously plastic injection quality content be 5% florfenicol injection (available commercially available
Finished medicines), injection dosage is 0.2～0.5ml/kg body weight.
Embodiment two
As in Figure 3-5, a kind of employing said method carries out mandarin sturgeon sex identification used tool, including first handle 4
With second handle 5, the middle part of first handle 4 and second handle 5 is flexibly connected by bolt 7, first handle 4 and second-hand
The top of handle 5 is flared out with vertical to a in a certain angle, and the top of first handle 4 and second handle 5 is respectively fixedly connected with support
Mouth sheet 8, support mouth sheet 8 is positioned at support mouth sheet 8 middle side edge with the junction of handle.
The angle of angle a is 15～20 degree.
Support mouth sheet 8 is the square lamellar body of a size of length of side 2 centimetres.
The above is only the non-limiting embodiment of the present invention, for the person of ordinary skill of the art, not
On the premise of departing from the invention design and not making creative work, it is also possible to making some deformation and improvement, these are all
Belong to protection scope of the present invention.
Claims (10)
1. a quick discriminating mandarin sturgeon property method for distinguishing, it is characterised in that the method comprises the following steps:
Step 1: supporting temporarily of mandarin sturgeon to be identified prepares: is put in storage pond, holding pond by mandarin sturgeon to be identified and supports temporarily, when supporting temporarily
Between be 2～3 days, support temporarily period and stop eating, and ensure good to support water water quality temporarily；
Step 2: configuration anesthesia liquid；
Step 3: the anesthesia of mandarin sturgeon to be identified: mandarin sturgeon to be identified is put into anesthesia case and carries out anaesthetizing until fish body enters deep
Degree narcotism；
Step 4: abdominal incision: take out mandarin sturgeon to be identified, slightly biased between the pectoral fin (2) and abdomeinal fin (3) of mandarin sturgeon to be identified
To the side of pectoral fin, along abdomen hone lamella upper limb at the opening (1) that side oddi's sphincteroplasty is 3～4 centimetres；
Step 5: sex abnormality: utilize special instrument of dehiscing, struts the upper opening (1) cut of mandarin sturgeon to be identified, observes in it
The shape of portion's gonad, carries out sex abnormality according to the external appearance characteristic of male and female gonad；
Step 6: wound processes: sews up the wound of the mandarin sturgeon completing sex abnormality, and carries out anti-inflammatory treatment；
Step 7: the recovery after discriminating and recovery: multiple by carrying out in the step 6 mandarin sturgeon flowing water culture pond immediately after treatment
Soviet Union, and note following the tracks of the recovery situation observing wound in the cultivation in later stage, can be recovered treatment if desired；
I.e. complete the quick discriminating of mandarin sturgeon sex.
One the most according to claim 1 quickly differentiates mandarin sturgeon property method for distinguishing, it is characterised in that support water in step 1 temporarily
Water quality index be: DO ＞ 6.0mg/L, NH4 +＜ 0.5mg/L, NO2 -＜ 0.05mg/L, pH value 7.5～8.5.
One the most according to claim 1 quickly differentiates mandarin sturgeon property method for distinguishing, it is characterised in that in step 1, waits to reflect
The age of a fish >=6 age of other mandarin sturgeon.
One the most according to claim 1 quickly differentiates mandarin sturgeon property method for distinguishing, it is characterised in that: in step 2, preparation
Anesthesia liquid be concentration be the Oleum Caryophylli of 80～120ppm or MS-222 that concentration is 80～100ppm.
One the most according to claim 1 quickly differentiates mandarin sturgeon property method for distinguishing, it is characterised in that: in step 4, cut
Pectoral fin (2) and abdomeinal fin (3) that opening (1) position is mandarin sturgeon between, and between pectoral fin (2) and abdomeinal fin (3) center line (6) away from
It it is 5～15 centimetres from d.
One the most according to claim 1 quickly differentiates mandarin sturgeon property method for distinguishing, it is characterised in that: the discriminating of step 5
Operating process controlled within the Anesthetic safety time of 5 minutes.
One the most according to claim 1 quickly differentiates mandarin sturgeon property method for distinguishing, it is characterised in that the antiinflammatory of step 6 is controlled
Treatment method is: smearing the mixture of erythromycin ointment and YUNNAN BAIYAO at wound, plastic injection quality content is the fluorobenzene of 5% simultaneously
Buddhist nun examines injection, and injection dosage is 0.2～0.5ml/kg body weight.
8. use the method according to any one of the claims 1-7 to carry out a mandarin sturgeon sex identification used tool, its
Being characterised by: include first handle (4) and second handle (5), the middle part of first handle (4) and second handle (5) is passed through
Bolt (7) is flexibly connected, and the top of first handle (4) and second handle (5) is flared out with vertical to a in a certain angle, the
The top of one handle (4) and second handle (5) is respectively fixedly connected with support mouth sheet (8), and support mouth sheet (8) is positioned at the junction of handle
Support mouth sheet (8) middle side edge.
Instrument the most according to claim 8, it is characterised in that: the angle of angle a is 15～20 degree.
Instrument the most according to claim 8, it is characterised in that: support mouth sheet (8) is the square of a size of length of side 2 centimetres
Lamellar body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610431873.7A CN106069901B (en) | 2016-06-17 | 2016-06-17 | Method for rapidly identifying sex of Acipenser sinensis and tool used by same |
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|---|---|---|---|
| CN201610431873.7A CN106069901B (en) | 2016-06-17 | 2016-06-17 | Method for rapidly identifying sex of Acipenser sinensis and tool used by same |
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| CN106069901B CN106069901B (en) | 2021-11-09 |
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Cited By (1)
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| CN111763723A (en) * | 2020-06-09 | 2020-10-13 | 中国长江三峡集团有限公司中华鲟研究所 | Nondestructive sex identification method for Chinese sturgeons |
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