CN102068429B - Application of fadrozole in inducing transformation of differentiated ovary of tilapia mossambica into functional testis and induction method thereof - Google Patents
Application of fadrozole in inducing transformation of differentiated ovary of tilapia mossambica into functional testis and induction method thereof Download PDFInfo
- Publication number
- CN102068429B CN102068429B CN201010609414A CN201010609414A CN102068429B CN 102068429 B CN102068429 B CN 102068429B CN 201010609414 A CN201010609414 A CN 201010609414A CN 201010609414 A CN201010609414 A CN 201010609414A CN 102068429 B CN102068429 B CN 102068429B
- Authority
- CN
- China
- Prior art keywords
- fadrozole
- ovary
- tilapia
- tilapia mossambica
- differentiated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention belongs to the technical field of inheritance breeding of fishes and relates to the application of fadrozole in inducing the transformation of the differentiated ovary of tilapia mossambica into functional testis and a method for inducting the transformation of the differentiated ovary of tilapia mossambica into the functional testis. In the method, a fadrozole-containing bait is continuously fed to female tilapia mossambica of which the sex gland has been differentiated into an ovary until the ovary is transformed into the functional testis. Fadrozole serving as an aromatase inhibitor is used for inducing the transformation of the differentiated ovary of tilapia mossambica into the functional testis, so that re-differentiation of the differentiated ovaries of all vertebrates into the functional testis is realized through a manual factor apart from natural reversed types, a new method for inducing the reversion of tilapia mossambica from a female individual into a male individual is established, a reliable experiment model is provided for researches on the molecular mechanism of the determination and differentiation of the gender of tilapia mossambica, and a new technical measure for the gender control of tilapia mossambica and single-gender breeding is established.
Description
Technical field
The invention belongs to the fish genetic breeding technical field, relate to arimedex fadrozole (Fadrozole) and change application in the functional type spermary into inducing tilapia to break up ovary, and utilize fadrozole to induce tilapia to break up the method that ovary changes the functional type spermary into.
Background technology
Tilapia claims African crucian again, excellent characteristic such as have that feeding habits are assorted, growth is fast, breeding is strong, thorn is few between delicious flavour and flesh, now become one of worldwide main cultured fishes, main breed variety has Mozambique tilapia, bolti, Oreochromis aureus and gift tilapia etc.In recent years, global tilapia cultured output cumulative year after year, China's tilapia output by 3.5 ten thousand tons of 1,130,000 tons of rising to 2007 of less than in 1987, also were expected to reach 1,200,000 tons in 43.5%, 2010 year that accounts for world wide production.At present, China has become the whole world maximum tilapia manufacturing country and exported country.
In the breeding process of tilapia, because 3 months gonads of raun obviously are divided into ovary, reach sexual maturity about 8 months, will breed once in every 15-20 days, will in the pond, produce a large amount of little tilapias, and little tilapia can compete nutrient substance, dissolved oxygen and space, and can not grow up to commercial specification at the end of the year, thereby can influence output and benefit that tilapia is cultured greatly.Therefore, if can be in breeding process the sex of Artificial Control tilapia, female tilapia is carried out sexual inversion, will improve the cultured output of tilapia greatly, reduce aquaculture cost, increase economic efficiency.
At present, it is to carry out HORMONE TREATMENT in the sensitive period of tilapia Sex determination and differentiation that tilapia is carried out the sexual inversion great majority, to induce sexual inversion completely.But because the sensitive period of tilapia Sex determination and differentiation very short (only hatching the back 3-5 days usually), be not easy to hold, and this macrura reevesii body is less, vulnerable, HORMONE TREATMENT can produce the fish body and necessarily damages even cause the death of fish body, thereby the pick-up rate of sexual inversion fish is reduced greatly.And how by anthropic factor after female tilapia gonad obviously is divided into ovary, induce it to change the functional type spermary into again, do not see bibliographical information as yet.
Aromatase is the synthetic terminal rate-limiting enzyme of steroid hormone, and it is estrogenic synthetic to participate in regulation and control directly.Bibliographical information is arranged, and before the differentiation of Fish gonad, it is synthetic that the injection arimedex can be blocked fish body inner estrogen, is that female individuality reverses to phenotypic sex is male individuality with genetic sex, and making gonad development is functional spermary.But after the raun gonad had been divided into ovary, can arimedex also induce it to break up ovary to change the functional type spermary into, do not appear in the newspapers as yet both at home and abroad up to now.
Summary of the invention
In view of this, whether the objective of the invention is to investigate arimedex can induce tilapia to break up ovary to change the functional type spermary into, thereby set up and a kind ofly induce tilapia by the new method of female individuals to the male sexual inversion, for the molecular mechanism of studying tilapia Sex determination and differentiation provides reliable experimental model, open up new technological approaches for tilapia sex controll and unisexuality breeding research simultaneously.
For achieving the above object, the present invention investigated arimedex fadrozole (Fadrozole) to induce bolti (
Oreochromis niloticus) broken up the influence that ovary transforms to the functional type spermary, the result shows, the 3 month female boltis that obviously are divided into ovary to gonad are thrown something and fed and contain the bait of fadrozole, fadrozole content is 400 μ g/g, threw something and fed continuously 90 days, and can induce bolti to break up ovary and change the functional type spermary into.Thus, the invention provides following technical scheme:
1, fadrozole changes application in the functional type spermary into inducing tilapia to break up ovary.
Further, described tilapia is a bolti.
2, utilizing fadrozole to induce tilapia to break up the method that ovary changes the functional type spermary into, is to throw something and feed continuously to the female tilapia that gonad has been divided into ovary to contain the bait of fadrozole, changes the functional type spermary into until ovary.
When described tilapia was bolti, said method can be specially: throw something and feed to the female bolti at 3 monthly ages and contain the bait of fadrozole, fadrozole content is 400 μ g/g, throws something and feeds continuously 90 days.
3, be used to induce tilapia to break up the bait that ovary changes the functional type spermary into, wherein contain fadrozole.
When described tilapia was bolti, the content of fadrozole was 400 μ g/g in the above-mentioned bait.
Beneficial effect of the present invention is: the present invention utilizes the arimedex fadrozole to induce tilapia to break up ovary to change functional spermary into, this is whole vertebrates is reversed kind except natural sex, realize that by anthropic factor having broken up ovary is divided into the functional type spermary first, set up and induced tilapia by the new method of female individuals to the male sexual inversion, not only the molecular mechanism for research tilapia Sex determination and differentiation provides reliable experimental model, and opened up new technological approaches for tilapia sex controll and unisexuality breeding, suit in tilapia and other cultured fishes, to apply, to cultured fishes sex controll and the significant and using value of unisexuality breeding.
Description of drawings
Fig. 1 is that the throw something and feed bait that contains fadrozole induces the gonadal tissue of different phase in the 3 month female bolti sexual inversion processes to observe (H.E dyeing), and wherein scheming A is normal raun ovarian structures of 3 monthly ages; Figure B is 3 monthly ages normal milter spermary structures; Figure C is the gonad structure of normal raun of 3 monthly ages after fadrozole is induced 70 days; Figure D is the gonad structure of normal raun of 3 monthly ages after fadrozole is induced 90 days; The scale of figure A and figure B is 20 μ m, and the scale of figure C and figure D is 10 μ m.
The specific embodiment
In order to make the purpose, technical solutions and advantages of the present invention clearer, the preferred embodiments of the present invention are described in detail below in conjunction with accompanying drawing.
1, contains the preparation of the bait of fadrozole
Take by weighing fadrozole 0.5g, make the mother solution that concentration is 10mg/ml with dehydrated alcohol 50ml dissolving, draw above-mentioned mother solution 8ml again, be diluted to 100ml with dehydrated alcohol, add among the bolti breeding bait 200g, stirring and evenly mixing dries or 37 ℃ of oven dry naturally again, promptly get the bait that contains fadrozole, fadrozole content is 400 μ g/g.Cofabrication blank bait adds dehydrated alcohol 100ml in bolti breeding bait 200g, stirring and evenly mixing dries or 37 ℃ of oven dry, promptly naturally.
2, throw something and feed and induce bolti to break up ovary to change functional spermary into
With false milter of bolti (XX ♂) and normal bolti female (XX ♀) breeding, obtain complete female bolti population, the normal bait of throwing something and feeding is supported it to 3 monthly ages in the circulating water culture system of 27 ℃ of water temperatures, be divided into blank group and experimental group at random, the throw something and feed blank bait of above-mentioned preparation of blank group, the throw something and feed bait that contains fadrozole of above-mentioned preparation of experimental group was thrown something and fed 90 days continuously.
3, bolti has broken up the detection that ovary changes spermary into
(1) microscopically microscopy seminal fluid
The fadrozole inductivity of learning from else's experience reverses the experimental group bolti after 90 days, gently presses its abdominal part, collects seminal fluid, adopts dull and stereotyped slide glass method, drips 1 experiment liquid on dull and stereotyped slide glass, dips in the dissecting needle needle point and gets a little seminal fluid and mix well with testing liquid, observes under low power lens.The result shows, contains a large amount of sperms in the experimental group bolti seminal fluid, illustrates that the ovary of female bolti has changed spermary into.
(2) histological observation gonad structure
Respectively before the fadrozole inductivity reverses and inductivity reverse after 70 days and 90 days, dissect the gonad that takes out blank group and experimental group bolti, Bouin ' s is liquid-solid fixed, paraffin section, through ethanol dehydrations at different levels, dimethylbenzene is transparent and paraffin embedding, do continuous crosscut 5 μ m section, H.E dyeing, optical microscope is observed its gonad structure down.The result as shown in Figure 1,3 the monthly age normal female bolti gonad in contain a large amount of oogoniums and oocyte at different levels, illustrate that gonad obviously is divided into ovary (Figure 1A); Contrast 3 the monthly age normal male bolti gonad, as seen contain a large amount of spermatogoniums, spermatocyte at different levels, spermatid and sperm gonad in its gonad, illustrate that gonad obviously is divided into spermary (Figure 1B); 3 the monthly age normal female bolti after the fadrozole inductivity reverses 70 days, its gonad major part changes the spermary structure into, contain spermatogonium, spermatocyte at different levels and spermatid, only have the oocyte (Fig. 1 C) of degenerating in the back and the health junction of gonad; After the fadrozole inductivity reversed 90 days, its gonad changed the spermary structure fully into, contains spermatogonium, spermatocyte at different levels and spermatid (Fig. 1 D).
4, the functional checking of the spermary of sexual inversion milter
(1) the spermary function of artificial insemination checking sexual inversion milter
Get the inductive sexual inversion milter of above-mentioned fadrozole, gently press its abdominal part, collect seminal fluid; In addition, collect the female bolti ovum that normal development is about to lay eggs; Seminal fluid and the ovum collected are mixed, adopt the dry method insemination techniques to carry out artificial insemination, germ cell is hatched in the circulating water culture system of 27 ℃ of water temperatures.Found that above-mentioned germ cell can hatch the filial generation bolti of normal development, show through the inductive spermary that is come by the ovary transformation of fadrozole to have normal function, the sperm of generation can normal fertilization.
(2) raise together with hybridization checking spermary function with normal raun
The inductive sexual inversion milter of above-mentioned fadrozole is mixed raising, the normal bait of throwing something and feeding, water temperature 26-27 ℃, natural light cycle with the normal female bolti of sexual maturity and gonad development.Found that above-mentioned female, the milter of raising together with can breed the filial generation bolti of normal development, show through fadrozole is inductive to change and the sperm that spermary produced that comes can normal fertilization, breed the offspring by ovary.
Comprehensive above-mentioned experimental result, can draw as drawing a conclusion: the arimedex fadrozole can be used in induces bolti to break up ovary to change functional spermary into, in view of similar performance between the different cultivars tilapia, therefore, those skilled in the art are according to record of the present invention, can know the present invention by inference and also be applicable to the tilapia of other kind, be that fadrozole can be used in and induces tilapia to break up ovary to change functional spermary into, its abductive approach is to throw something and feed continuously to the female tilapia that gonad has been divided into ovary to contain the bait of fadrozole, changes the functional type spermary into until ovary.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by invention has been described with reference to the preferred embodiments of the present invention, but those of ordinary skill in the art is to be understood that, can make various changes to it in the form and details, and the spirit and scope of the present invention that do not depart from appended claims and limited.
Claims (5)
1. fadrozole changes application in the functional type spermary into inducing tilapia to break up ovary.
2. the application of fadrozole according to claim 1 is characterized in that: described tilapia is a bolti.
3. utilize fadrozole to induce tilapia to break up the method that ovary changes the functional type spermary into, it is characterized in that: the female tilapia that has been divided into ovary to gonad is thrown something and fed continuously and contains the bait of fadrozole, changes the functional type spermary into until ovary.
4. the fadrozole that utilizes according to claim 3 induces tilapia to break up the method that ovary changes the functional type spermary into, and it is characterized in that: described tilapia is a bolti.
5. the fadrozole that utilizes according to claim 4 induces tilapia to break up the method that ovary changes the functional type spermary into, it is characterized in that: throw something and feed to the female bolti at 3 monthly ages and contain the bait of fadrozole, fadrozole content is 400 μ g/g, throws something and feeds continuously 90 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010609414A CN102068429B (en) | 2010-12-28 | 2010-12-28 | Application of fadrozole in inducing transformation of differentiated ovary of tilapia mossambica into functional testis and induction method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010609414A CN102068429B (en) | 2010-12-28 | 2010-12-28 | Application of fadrozole in inducing transformation of differentiated ovary of tilapia mossambica into functional testis and induction method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102068429A CN102068429A (en) | 2011-05-25 |
| CN102068429B true CN102068429B (en) | 2011-12-14 |
Family
ID=44027309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010609414A Active CN102068429B (en) | 2010-12-28 | 2010-12-28 | Application of fadrozole in inducing transformation of differentiated ovary of tilapia mossambica into functional testis and induction method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102068429B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112262794A (en) * | 2020-10-24 | 2021-01-26 | 武汉市农业科学院 | Method for sustainable large-scale production of all-female mandarin fish |
| CN116391654B (en) * | 2023-03-30 | 2023-11-03 | 中国海洋大学 | Large-scale hybridization breeding method for hermaphrodite scallops |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EA005931B1 (en) * | 2000-05-15 | 2005-08-25 | Фармация Италия С.П.А. | Aromatase inhibitors and monoclonal anti-her2 antibodies as antitumors agents |
| GB0017635D0 (en) * | 2000-07-18 | 2000-09-06 | Pharmacia & Upjohn Spa | Antitumor combined therapy |
| CN1273132C (en) * | 2004-08-24 | 2006-09-06 | 中山大学 | Aromatase inhibitor |
| CN1282487C (en) * | 2004-09-01 | 2006-11-01 | 中山大学 | Sustained release medicine for artificially inducing sex reversal of grouper and its preparation method |
-
2010
- 2010-12-28 CN CN201010609414A patent/CN102068429B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102068429A (en) | 2011-05-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109644902A (en) | A kind of Lhasa schizothoracin artificial breeding method | |
| CN101491228B (en) | Sea no-nucleus pearl incubation method | |
| CN103931525B (en) | The artificial propagation of the naked skin-carp meat in Xinjiang and fry rearing method | |
| CN103875574A (en) | Breeding method for amphiprotic fertile autotetraploid crucian carps, establishment method for strains of crucian carps and breeding method for triploid fishes | |
| CN101743924B (en) | In-vitro hatching method for freshwater lobster zygote | |
| CN102144595A (en) | Breeding method of loach | |
| Peixoto et al. | Effects of age and size on reproductive performance of captive Farfantepenaeus paulensis broodstock | |
| CN104221952B (en) | A kind of Chalcalbumus chalcoides aralensis artificial fecundation method | |
| CN102379256A (en) | Ring-duct net cage for breeding of oriental weatherfish | |
| Lee et al. | Induction of primary male in juvenile red spotted grouper Epinephelus akaara by immersion of 17α-methyltestosterone | |
| CN106222204A (en) | A kind of method of Monopterus albus (Zuiew) gene editing | |
| CN101416613A (en) | The method of feminizing of WZ ♀-ZZ ♂ type Oreochromis aureus ZZ zygote | |
| CN108293924A (en) | A kind of artificial fecundation method of Ptychobarbus dipogon | |
| CN102068429B (en) | Application of fadrozole in inducing transformation of differentiated ovary of tilapia mossambica into functional testis and induction method thereof | |
| CN104255608B (en) | Artificial spawning method for thick-lipped nude hemibarbus labeo | |
| Miyanishi et al. | Reproductive farming technology in Japanese eel and chub mackerel | |
| CN105557586A (en) | Hybrid breeding method for female Siberian sturgeon and male Amur sturgeon | |
| CN106614188A (en) | Non-directional mating litopenaeus vannamei full-sib family batch preparation method | |
| CN109496922A (en) | A kind of producing method for seed of hydrostatic pressing induction rainbow trout tetraploid | |
| CN109548712B (en) | Method for breeding female triploid zebra fish by using heat shock method | |
| Degani et al. | Vitellogenin level in the plasma of russian sturgeon (Acipenser gueldenstaedtii) northern Israel | |
| CN110139557A (en) | The cultivation water of Anguilliforme fish and the breeding method of Anguilliforme fish | |
| Fairchild et al. | Gonadal development and differentiation in cultured juvenile winter flounder, Pseudopleuronectes americanus | |
| CN107926770A (en) | Epinephelus akaara and epinephelus lanceolatus fish distant hybrid breeding method | |
| CN100490638C (en) | Method for synchronously developing gonads of salamander parents |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |