CN106069901B - Method for rapidly identifying sex of Acipenser sinensis and tool used by same - Google Patents
Method for rapidly identifying sex of Acipenser sinensis and tool used by same Download PDFInfo
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- CN106069901B CN106069901B CN201610431873.7A CN201610431873A CN106069901B CN 106069901 B CN106069901 B CN 106069901B CN 201610431873 A CN201610431873 A CN 201610431873A CN 106069901 B CN106069901 B CN 106069901B
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Abstract
A method for rapidly identifying the sex of Acipenser sinensis comprises the following steps: step 1: preparing for temporary culture of the Chinese sturgeons to be identified; step 2: preparing anesthetic liquid; and step 3: anesthesia of the Acipenser sinensis to be identified; and 4, step 4: an abdominal incision; and 5: sex identification; step 6: treating the wound; and 7: resuscitation and recovery after identification; thus completing the rapid identification of the sex of the Acipenser sinensis. The method and the tool for rapidly identifying the sex of the Chinese sturgeon can solve the problem that the sex identification method of the Chinese sturgeon in the early stage of sexual maturity is lacked, and can rapidly, efficiently and accurately identify the sex of the Chinese sturgeon; convenient operation and strong practicability, and meets the requirements of sex identification in the works of Chinese sturgeon culture and scientific research.
Description
Technical Field
The invention relates to the field of aquaculture, in particular to a method for rapidly identifying the sex of an Acipenser sinensis and a tool used by the same.
Background
Chinese sturgeon is a large-scale migratory fish, is one of the most original and rarest species in the existing fishes in the world, is also the first-level important wild animal protection in China, and enjoys the reputation of 'pandas in water'. The sexual maturity time of the Chinese sturgeons is long, the first time maturity time of female Chinese sturgeons is generally 14-26 years (18 years on average), and the first time maturity time of male Chinese sturgeons is generally 8-18 years (12 years on average). In the process of cultivating the Chinese sturgeons, all the fishes are all mixedly cultivated before the sexual maturity age is reached due to the lack of an effective method for identifying males and females; only when the sexual maturity age is approached and the gonadal characteristics are obvious, the male and the female can be distinguished by means of technologies such as B-ultrasound, puncture and the like. When the gonad of the adult Chinese sturgeon is identified by using the B-ultrasonic technology, the requirement on the development of the gonad of the adult Chinese sturgeon is high, the adult Chinese sturgeon can be effectively identified only under the condition that the characteristics of the male and female gonads in a B-ultrasonic image are very obvious, the identification rate of the method is low, and the technical requirement on operators is high; when the puncturing method is used for sex identification of the Acipenser sinensis, the sex identification can be accurately carried out only when the content features obtained by puncturing are obvious (female content generally has obvious granular eggs, and male content generally presents white blocks or seminal plasma). The methods cannot accurately identify the sexes of the low-age Chinese sturgeons, and are not beneficial to the optimization of the group structure of the Chinese sturgeon artificial culture group, the targeted culture of the parent fishes and the development of species protection work.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for rapidly identifying the sex of the Chinese sturgeon and a tool used by the method, which can solve the problem that the sex identification method of the Chinese sturgeon in the early sexual maturity stage is lacked, can identify the sex of the Chinese sturgeon in the early sexual maturity stage (more than 6 years old), overcomes the defect that the sex identification can be carried out only after the Chinese sturgeon enters the sexual maturity stage (the average female fish is 18 years, and the average male fish is 12 years) in the existing method, and is rapid, efficient and accurate; the sex identification method is convenient to operate and extremely high in practicability, meets the requirement of sex identification in the work of breeding and scientific research of the Chinese sturgeons, has small damage to the fish while ensuring high accuracy, and is one of effective ways for identifying the sex of the Chinese sturgeons.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a method for rapidly identifying the sex of Acipenser sinensis comprises the following steps:
step 1: preparing for temporary culture of the Chinese sturgeons to be identified: putting the Chinese sturgeons to be identified into a temporary culture pond for temporary culture, wherein the temporary culture time is 2-3 days, and stopping eating during the temporary culture period, and ensuring good temporary culture water quality;
step 2: preparing anesthetic liquid;
and step 3: anesthesia of the Acipenser sinensis to be identified: placing the Chinese sturgeon to be identified into an anesthesia box for anesthesia until the fish body enters a deep anesthesia state;
and 4, step 4: abdominal incision: taking out the Chinese sturgeon to be identified, slightly inclining the position between the pectoral fin and the ventral fin of the Chinese sturgeon to be identified to one side of the pectoral fin, and cutting an opening with the length of 3-4 cm along the upper edge of the ventral bone plate on the body side;
and 5: and (3) sex identification: opening the cut opening of the Acipenser sinensis to be identified by using a special opening tool, observing the shape of the internal gonad of the Acipenser sinensis, and identifying the sex according to the appearance characteristics of the male and female gonads;
step 6: treating wounds: sewing the wound of the Acipenser sinensis with the sex identification completed, and performing anti-inflammatory treatment;
and 7: resuscitation and recovery after identification: recovering the treated Chinese sturgeons in the flow culture pond immediately, and paying attention to follow and observe the recovery condition of the wound in later culture, and performing recovery treatment if necessary;
thus completing the rapid identification of the sex of the Acipenser sinensis.
The water quality indexes of the temporarily-cultured water in the step 1 are as follows: DO > 6.0mg/L, NH4 +＜0.5mg/L,NO2 -Less than 0.05mg/L and a pH value of 7.5-8.5.
In the step 1, the age of the Chinese sturgeon to be identified is more than or equal to 6 years.
In the step 2, the prepared anesthetic liquid is clove oil with the concentration of 80-120 ppm or MS-222 with the concentration of 80-100 ppm.
In the step 4, the cut opening position is between the pectoral fin and the ventral fin of the Acipenser sinensis, and the distance d between the cut opening position and the midline between the pectoral fin and the ventral fin is 5-15 cm.
The authentication process of step 5 is controlled within 5 minutes of safe time for anesthesia.
The anti-inflammation treatment method in the step 6 comprises the following steps: the mixture of erythromycin ointment and Yunnan white drug powder is smeared on the wound, and florfenicol injection with the mass content of 5% is injected at the same time, and the injection dosage is 0.2-0.5 ml/kg of body weight.
The tool for identifying the sex of the Chinese sturgeon by adopting the method comprises a first handle and a second handle, wherein the middle parts of the first handle and the second handle are movably connected through a bolt, the upper parts of the first handle and the second handle are outwards opened and vertically form a certain included angle a, the top ends of the first handle and the second handle are respectively and fixedly connected with a mouth supporting piece, and the connecting part of the mouth supporting piece and the handle is positioned in the middle of the side edge of the mouth supporting piece.
The included angle a is 15-20 degrees.
The opening supporting sheet is a square sheet body with the size of 2 cm on side.
The invention provides a method for rapidly identifying the sex of Chinese sturgeons and a tool used by the method, and provides the method and the special tool for rapidly identifying the sex of the Chinese sturgeons for the first time, so that the problem that the method for identifying the sex of the Chinese sturgeons in the early sexual maturity stage is lacked can be solved, the sex identification of the Chinese sturgeons in the early sexual maturity stage (more than 6 years old) can be carried out, the defect that the sex identification can be carried out only after the Chinese sturgeons enter sexual maturity (the average female fish is 18 years, and the average male fish is 12 years) in the existing method is overcome, and the method is rapid, efficient and accurate; the sex identification method is convenient to operate and extremely high in practicability, meets the requirement of sex identification in the work of breeding and scientific research of the Chinese sturgeons, has small damage to the fish while ensuring high accuracy, and is one of effective ways for identifying the sex of the Chinese sturgeons.
By adopting the method and the tool for sex identification of 36 Chinese sturgeons, 15 male and female Chinese sturgeons are identified, 20 male Chinese sturgeons have unobvious gonadal characteristics and can not be distinguished, and 1 Chinese sturgeon is identified, and the identification rate of the female and male sturgeons by adopting the method is 97%.
Drawings
The invention is further illustrated by the following examples in conjunction with the accompanying drawings:
FIG. 1 is a schematic diagram of the abdominal incision of Acipenser sinensis in step 4 of the method of the present invention;
FIG. 2 is a schematic representation of the appearance of the male and female gonads observed in step 5 of the method of the present invention;
FIG. 3 is a schematic structural view of a tool used in the present invention, wherein the two port plates are in a closed state;
FIG. 4 is a schematic structural view of a tool used in the present invention, with the two port plates in an open position;
fig. 5 is a schematic diagram of the components of a tool used in the present invention.
Detailed Description
Example one
A method for rapidly identifying the sex of Acipenser sinensis comprises the following steps:
step 1: preparing for temporary culture of the Chinese sturgeons to be identified: putting the Chinese sturgeons to be identified into a temporary culture pond for temporary culture, wherein the temporary culture time is 2-3 days, and stopping eating during the temporary culture period, and ensuring good temporary culture water quality;
step 2: preparing anesthetic liquid;
and step 3: anesthesia of the Acipenser sinensis to be identified: placing the Chinese sturgeon to be identified into an anesthesia box for anesthesia until the fish body enters a deep anesthesia state (the fish body can enter the deep anesthesia state in about 5-8 minutes),
the specification of the anesthesia box is 4 meters multiplied by 2 meters multiplied by 1 meter, the total volume is 8 cubic meters, and 0.5 meter deep water with the water volume of 48 cubic meters is put into the anesthesia box;
and 4, step 4: abdominal incision: taking out the Chinese sturgeon to be identified, slightly inclining the position between the pectoral fin 2 and the ventral fin 3 of the Chinese sturgeon to be identified to one side of the pectoral fin, and cutting an opening 1 with the length of 3-4 cm along the upper edge of the ventral bone plate on the body side, as shown in figure 1;
and 5: and (3) sex identification: opening the cut opening 1 of the Acipenser sinensis to be identified by using a special opening tool, observing the shape of the internal gonad, identifying the sex according to the appearance characteristics of the male and female gonads, as shown in figure 2,
female gonad characteristics: the gonad is in a fine-leaf branch shape and has the color characteristics of light yellow, pink and the like;
male gonadal characteristics: the gonad is divided into a plurality of small blocks, the surface is smooth, and the gonad has the color characteristics of light pink, white and the like;
step 6: treating wounds: sewing the wound of the Acipenser sinensis with the sex identification completed, and performing anti-inflammatory treatment;
and 7: resuscitation and recovery after identification: recovering the treated Chinese sturgeons in the flow culture pond immediately, and paying attention to follow and observe the recovery condition of the wound in later culture, and performing recovery treatment if necessary;
thus completing the rapid identification of the sex of the Acipenser sinensis.
The water quality indexes of the temporarily-cultured water in the step 1 are as follows: DO > 6.0mg/L, NH4 +＜0.5mg/L,NO2 -Less than 0.05mg/L, the pH value of 7.5-8.5 and the water temperature of 15-16 ℃.
In the step 1, the age of the Chinese sturgeon to be identified is more than or equal to 6 years.
In the step 2, the prepared anesthetic liquid is clove oil with the concentration of 80-120 ppm or MS-222 with the concentration of 80-100 ppm.
In the step 4, the position of the cut opening 1 is between the pectoral fin 2 and the ventral fin 3 of the Acipenser sinensis, and the distance d from the middle line 6 between the pectoral fin 2 and the ventral fin 3 is 5-15 cm.
The authentication process of step 5 is controlled within 5 minutes of safe time for anesthesia.
The anti-inflammation treatment method in the step 6 comprises the following steps: the mixture of the erythromycin ointment and the Yunnan white powder is smeared on the wound (the mass ratio of the erythromycin ointment to the Yunnan white powder is 3:1), and the florfenicol injection (which can be a commercially available finished product medicine) with the mass content of 5% is injected at the same time, wherein the injection dose is 0.2-0.5 ml/kg of body weight.
Example two
As shown in fig. 3-5, a tool for identifying the sex of the Acipenser sinensis by adopting the method comprises a first handle 4 and a second handle 5, wherein the middle parts of the first handle 4 and the second handle 5 are movably connected through a bolt 7, the upper parts of the first handle 4 and the second handle 5 are outwards opened and form a certain included angle a with the vertical direction, the top ends of the first handle 4 and the second handle 5 are respectively and fixedly connected with a mouth supporting piece 8, and the connecting part of the mouth supporting piece 8 and the handles is positioned in the middle of the side edge of the mouth supporting piece 8.
The included angle a is 15-20 degrees.
The opening supporting sheet 8 is a square sheet body with the size of 2 cm on side.
The above description is only a non-limiting embodiment of the present invention, and it will be apparent to those skilled in the art that various changes and modifications may be made without departing from the inventive concept and without inventive step, and these changes and modifications are all within the scope of the present invention.
Claims (10)
1. A method for rapidly identifying the sex of Acipenser sinensis is characterized by comprising the following steps:
step 1: preparing for temporary culture of the Chinese sturgeons to be identified: putting the Chinese sturgeons to be identified into a temporary culture pond for temporary culture, wherein the temporary culture time is 2-3 days, and stopping eating during the temporary culture period, and ensuring good temporary culture water quality;
step 2: preparing anesthetic liquid;
and step 3: anesthesia of the Acipenser sinensis to be identified: placing the Chinese sturgeon to be identified into an anesthesia box for anesthesia until the fish body enters a deep anesthesia state;
and 4, step 4: abdominal incision: taking out the Chinese sturgeon to be identified, slightly deviating one side of the pectoral fin between the pectoral fin (2) and the ventral fin (3) of the Chinese sturgeon to be identified, and cutting an opening (1) with the length of 3-4 cm along the upper edge of the ventral bone plate on the body side;
and 5: and (3) sex identification: opening an opening (1) cut on the Acipenser sinensis to be identified by using a special opening tool, observing the shape of the internal gonad of the Acipenser sinensis, and identifying the sex according to the appearance characteristics of the male and female gonads;
step 6: treating wounds: sewing the wound of the Acipenser sinensis with the sex identification completed, and performing anti-inflammatory treatment;
and 7: resuscitation and recovery after identification: recovering the treated Chinese sturgeons in the flow culture pond immediately, and paying attention to follow and observe the recovery condition of the wound in later culture, and performing recovery treatment if necessary;
thus completing the rapid identification of the sex of the Acipenser sinensis.
2. The method for rapidly identifying the sex of the Acipenser sinensis according to claim 1, wherein the water quality index of the temporarily-cultured water in the step 1 is as follows: DO > 6.0mg/L, NH4 +＜0.5mg/L, NO2 -Less than 0.05mg/L and a pH value of 7.5-8.5.
3. The method for rapidly identifying the sex of the Acipenser sinensis according to claim 1, wherein in the step 1, the age of the Acipenser sinensis to be identified is more than or equal to 6 years old.
4. The method for rapidly identifying the sex of the Acipenser sinensis according to claim 1, which is characterized in that: in the step 2, the prepared anesthetic liquid is clove oil with the concentration of 80-120 ppm or MS-222 with the concentration of 80-100 ppm.
5. The method for rapidly identifying the sex of the Acipenser sinensis according to claim 1, which is characterized in that: in the step 4, the position of the cut opening (1) is between the pectoral fin (2) and the ventral fin (3) of the Acipenser sinensis, and the distance d from the middle line (6) between the pectoral fin (2) and the ventral fin (3) is 5-15 cm.
6. The method for rapidly identifying the sex of the Acipenser sinensis according to claim 1, which is characterized in that: the authentication process of step 5 is controlled within 5 minutes of safe time for anesthesia.
7. The method for rapidly identifying the sex of the Acipenser sinensis according to claim 1, wherein the anti-inflammatory treatment method in the step 6 comprises the following steps: the mixture of erythromycin ointment and Yunnan white drug powder is smeared on the wound, and florfenicol injection with the mass content of 5% is injected at the same time, and the injection dosage is 0.2-0.5 ml/kg.
8. A tool for sexing an acipenser sinensis according to any one of claims 1 to 7, wherein the tool comprises: including first handle (4) and second handle (5), bolt (7) swing joint is passed through to the middle part of first handle (4) and second handle (5), and the upper portion of first handle (4) and second handle (5) is outwards opened and is certain contained angle a with perpendicular to being, and each fixedly connected with in top of first handle (4) and second handle (5) props a mouthful piece (8), props mouthful piece (8) and is located to prop a mouthful piece (8) side middle part with the junction of handle.
9. The tool of claim 8, wherein: the included angle a is 15-20 degrees.
10. The tool of claim 8, wherein: the opening supporting sheet (8) is a square sheet body with the side length of 2 cm.
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| CN111763723B (en) * | 2020-06-09 | 2023-03-31 | 中国长江三峡集团有限公司中华鲟研究所 | Nondestructive sex identification method for Chinese sturgeons |
| CN118303531A (en) * | 2024-05-11 | 2024-07-09 | 中国长江三峡集团有限公司中华鲟研究所 | Additive composition and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101647718A (en) * | 2008-08-12 | 2010-02-17 | 杭州千岛湖鲟龙科技开发有限公司 | Method for identifying male and female sturgeons by ultrasonic waves |
| CN105123579A (en) * | 2015-09-02 | 2015-12-09 | 中国长江三峡集团公司中华鲟研究所 | Sturgeon zygote incubation device |
| CN205671247U (en) * | 2016-06-17 | 2016-11-09 | 中国长江三峡集团公司中华鲟研究所 | For quickly differentiating the instrument of mandarin sturgeon sex |
| CN107182882A (en) * | 2017-05-31 | 2017-09-22 | 杭州千岛湖鲟龙科技股份有限公司 | A kind of low age sturgeon belly side opening Sex judging technology |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766495A (en) * | 2008-12-29 | 2010-07-07 | 中国水产科学研究院东海水产研究所 | Method for identifying sex of sturgeon by ultrasonic waves |
| CN101836611B (en) * | 2010-05-27 | 2013-04-24 | 中国长江三峡集团公司中华鲟研究所 | Method for regulating gonadogenesis of first-generation parent fish of Acipenser sinensis |
| CN102165929A (en) * | 2011-06-02 | 2011-08-31 | 广州市先步农业发展有限公司 | Artificial propagation method for sturgeon |
-
2016
- 2016-06-17 CN CN201610431873.7A patent/CN106069901B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101647718A (en) * | 2008-08-12 | 2010-02-17 | 杭州千岛湖鲟龙科技开发有限公司 | Method for identifying male and female sturgeons by ultrasonic waves |
| CN105123579A (en) * | 2015-09-02 | 2015-12-09 | 中国长江三峡集团公司中华鲟研究所 | Sturgeon zygote incubation device |
| CN205671247U (en) * | 2016-06-17 | 2016-11-09 | 中国长江三峡集团公司中华鲟研究所 | For quickly differentiating the instrument of mandarin sturgeon sex |
| CN107182882A (en) * | 2017-05-31 | 2017-09-22 | 杭州千岛湖鲟龙科技股份有限公司 | A kind of low age sturgeon belly side opening Sex judging technology |
Non-Patent Citations (2)
| Title |
|---|
| 中华鲟全人工繁殖技术研究;危起伟等;《中国水产科学》;20130131;第20卷(第1期);第1-10页 * |
| 长江干流长鳍吻鮈（Rhinogo ventralis）繁殖生物学;曲焕韬等;《渔业科学进展》;20160228;第37卷(第1期);第1-7页 * |
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