CN105994943A - Animal feed attractant and preparation method thereof - Google Patents

Abstract

The present invention belongs to the fields of microorganisms, fermentation and feed additives, and particularly relates to an animal feed attractant and a preparation method thereof. The attractant consists of the following components in weight percentages: 10%-50% of sodium glutamate, 10%-30% of disodium 5'-inosinate, 0.5%-5% of disodium guanylate, 2%-10% of amino acids, 2%-10% of organic acids, 0.5%-2% of fats and 0.5%-3% of aromatic compounds, and the sum of the weight percentage of each substance is 100%. The invention also provides a preparation method of the animal feed attractant. The method comprises the following steps: microorganism inoculating in a fermentation medium, seed culturing, fermenting, sterilizing, and concentrating/concentrating and drying. The obtained attractant can be liquid, paste or powder, and widely used in feed production process, and have food attracting and appetite promoting effects for animals and pets.

Description

A kind of animal feed phagostimulant and preparation method thereof

Technical field

The invention belongs to microorganism, fermentation and feed additive field, particularly to a kind of animal Feed eating belt agent and preparation method thereof.

Background method

The bad smell produced in conventional feed production process, easily affects animal palatability, from And affect the food-intake of animal.For improving the food-intake of animal, people are often in conventional feed Increase additive, though but these additives have the effect of delicate flavour, but nutritive value is little, has A little additives also can precipitate toxin in animal bodies, and these toxin are final by food chain structure Transfer to, on human body, affect health.

Summary of the invention

For above technical problem, the invention provides that one is of high nutritive value, food calling effect is strong, And the animal feed additive having no side effect, add additive to animal is daily raises by a certain amount of In material, feedstuff palatability can be improved, increase food-intake, promote that animal is mesotrophic to feedstuff Absorb.

In order to solve above technical problem, the present invention adopts the following technical scheme that a kind of animal is raised Material phagostimulant, its weight percent consists of: sodium glutamate 10%-50%, inosine monophosphate 10%-30%, guanosine monophosphate disodium 0.5%-5%, aminoacid 2%-10%, organic acid 2%-10%, fat Fat 0.5%-2% and aromatic compounds 0.5%-3%, the weight percent composition sum of each material It is 100%.Described aromatic compounds be those skilled in the art commonly use tannin, pigment And aromatics.

Further: in above-mentioned animal feed phagostimulant, described organic acid is malic acid, lemon At least one in lemon acid, lactic acid, quininic acid and acetic acid.

The method that present invention also offers above-mentioned animal feed phagostimulant, it includes inoculating strain Cultivating seeds, fermentation, sterilizing, the process of concentration/concentrate drying is carried out in fermentation medium. Described strain is at least one in glutamic acid bacillus, bacillus subtilis or corynebacterium ammoniagenes. Described fermentating controling condition is, inoculum concentration is 10 ± 1%, and temperature is 30-37 DEG C, and PH is 6.6-7.2, tank pressure is 0.05MPa, dissolved oxygen >=15%.Described fermentation medium percentage by weight Concentration proportioning be Semen Maydis starch 1.5-2%, sugared close 5-10%, potassium dihydrogen phosphate 0.1-0.3%, Ammonium sulfate 0.2-0.5%, magnesium sulfate 0.1-0.3%, ferrous sulfate 0.03-0.05%, sulphuric acid Manganese 0.03-0.05%, glucose 5-10%, surplus is water.Described concentration/concentrate drying is Refer to concentrate the 3-8 times of creamy product obtained through negative pressure 50-90 DEG C after fermentation liquid sterilizing；Or again Continue to be dried to obtain powder product by creamy product.Described fermentation refers to that corynebacterium ammoniagenes is fermented extremely The concentration of inosine monophosphate IMP reaches 3%-12.0%；Or Corynebacterium glutamicum/bacillus subtilis Bacterium fermentation reaches 5%-17.0% to fermentation liquid Glutamic Acid concentration；Or the two mixture.

In prepared by above-mentioned feedstuff food calling additive, the fermentation culture of described corynebacterium ammoniagenes Temperature is in temperature 30-37 DEG C, and the time of fermentation culture is 60-100h；Corynebacterium glutamicum or Person's fermentation of bacillus subtilis cultivation temperature is 32-41 DEG C；Two kinds of pure-blood ferment liquid mix in proportion After conjunction, continue fermentation to fermentation liquid glucose content close to 0.Product ammonia bar used by the present invention Bacterium, Corynebacterium glutamicum or bacillus subtilis are that art technology is approved also in food scope Known strain.

Beneficial effect: compared with prior art, the present invention prepares feedstuff food calling additive technology tool There is a following advantage:

(1) the glutamic acid bar that the feedstuff food calling additive cause nature of the present invention is widely present Bacterium, corynebacterium ammoniagenes, bacillus subtilis process, and production technology is simple, are a kind of green Color, eco-friendly feed additive；

Product the most of the present invention contains abundant aminoacid, organic acid, nucleotide and aromatic compound Thing, and there is the fresh effect of increasing, animal is had obvious food calling effect；

Product form the most of the present invention can be emulsus, paste, bulk, powdery etc., conveniently makes an addition to Follow-up feed production technology.

Even sodium glutamate and IMP or GMP the used time, it is possible to cover the abnormal flavour in feedstuff, change Kind feedstuff palatability, promotes that feed digestion absorbs, increases yield, almost to all of fowl Domestic animal, Fish, shrimps, shellfish all have the strongest food calling effect.In addition, nucleotide exists Animal body normal immunological function aspects is maintained to play an important role.First, suitable in feedstuff When adding nucleotide, the excrement bifidobacteria in animal intestinal can be made to preponderate, thus suppress The propagation of enteropahtogenic microganism；Secondly, the growth of intestinal and maturation need extraneous nucleotide Supply, makes enterocyte preferably breed, breaks up and maintain integrity.

Detailed description of the invention

Prepared by 1 seed liquor

Preparation Corynebacterium glutamicum, corynebacterium ammoniagenes/bacillus subtilis slant medium, training Supporting basigamy ratio is: yeast extract 0.5%, glucose 2%, potassium dihydrogen phosphate 0.1%, sulphuric acid Ammonium 0.2%, magnesium sulfate 0.1%, agar powder 1.5%；Strain is inoculated in slant medium, Cultivate 24h for 37 DEG C, with sterilized water, the lawn on inclined-plane is washed till sterilized three and solves in bottle, system Obtain bacteria suspension.

The preparation of 2 secondary seeds

Preparation secondary seed medium, proportioning is Semen Maydis pulp 0.5%, yeast extract 0.3%, Potassium dihydrogen phosphate 0.1%, ammonium sulfate 0.1%, magnesium sulfate 0.1%, grape sugar sugar 2%；After dissolving Enter fermentation tank constant volume, 125 DEG C of sterilizing 20min, be cooled to 37 DEG C and access seed bacteria suspension, send out Ferment gets final product subcultivation when being 0.3-0.5 to OD net added value；

3, prepared by feedstuff food calling additive mixed liquor

Fermentation base Semen Maydis starch 1.5-2%, sugared close 5-10%, potassium dihydrogen phosphate 0.1-0.3%, Ammonium sulfate 0.2-0.5%, magnesium sulfate 0.1-0.3%, ferrous sulfate 0.03-0.05%, sulphuric acid Manganese 0.03-0.05%, glucose 5-10%, surplus is water.Wherein molasses, glucose are by one Determine to divide after volume dissolves to disappear, after sterilization, pump into fermentation tank by fed-batch mode；Medium sterilization disappears Adjusting PH to 6-6.7 with ammonia after poison cooling, secondary seed solution is accessed by 10% volume, maintains and sends out Ferment tank pressure 0.05Mpa, tank temperature 30-37 DEG C, DO >=20%, by fermentation time and fermentation condition Control fermentation termination to fermentation liquid sodium glutamate content be 10-20% or IMP content be 6-9% Time, the sterilizing both are mixed in proportion obtain mixed liquor of can heating, mixed liquor is concentrated to After certain viscosity, it is spray-dried, obtains the animal feed phagostimulant of the present invention.

Preferably constituting as sodium glutamate 40%-48%, inosine monophosphate of its phagostimulant 25%-30%, guanosine monophosphate disodium 3.5%-5%, aminoacid 8%-10%, organic acid 5%-7%, fat Fat 0.5%-2% and aromatic compounds 1%-3%, the weight percent composition sum of each material is 100%.

4 products application

Product of the present invention is added to containing bean cake, wheat bran, cottonseed meal, Semen Maydis fibre with 8-15% ratio Animal is thrown something and fed by the fermented feed of dimension, Testa oryzae, powdered rice hulls etc..

Test example 1

Fructus Hordei Vulgaris wheat-middlings 300 parts, Semen Maydis powder 200 parts, bean dreg powder 200 parts, 180 parts of Testa oryzae, Fish flour 20 parts, lysine 2 parts, phytase 3 parts, cellulose close to obtain enzyme 5 parts, biological work Property bacterium 5 parts, compound vitamin 8 parts, Sal 6 parts, antifoggant 3 parts, product of the present invention 10 parts, as testing the impact that live pig is digested and assimilated by product of the present invention, choose 50 ± 3kg Live pig 100, is randomly divided into two groups, and matched group is without product of the present invention, and other raises bar Part is identical, raises contrast effect and see table:

	Experimental group (g)	Matched group (g)
			Daily gain	825±12	738±25
Feed to delivering day for sale	53±2	59±3

Test 2

Test uses 96 child care piglets (original body mass 12.00 ± 0.5kg), according to piglet The sow parity in source is close, the principle that male and female ratio is consistent, is assigned randomly to 4 groups, often Individual group 4 repetitions, 6 piglets of each repetition, share 16 circles.Piglet enters nursery house After by equal amount of mixture mixed feeding 3 days step by step of the former child care material fed and 4 kinds of testing materials； Starting formal test afterwards, feed 4 kinds of daily rations respectively, experimental period is 4 weeks, and result of the test is shown in Following table, as can be known from the results, feed eating belt agent of the present invention has obvious effect to the weightening finish of piglet food calling.

Claims (8)

1. an animal feed phagostimulant, its weight percent consists of: sodium glutamate 10%-50%, Inosine monophosphate 10%-30%, guanosine monophosphate disodium 0.5%-5%, aminoacid 2%-10%, organic acid 2%-10%, fat 0.5%-2% and aromatic compounds 0.5%-3%, the weight hundred of each material Being grouped into sum is 100%.

Animal feed phagostimulant the most according to claim 1, it is characterised in that: described Organic acid be at least one in malic acid, citric acid, lactic acid, quininic acid and acetic acid.

3. the method preparing animal feed phagostimulant described in claim 1 or 2, its It is characterised by: it includes being inoculated in strain fermentation medium and carries out cultivating seeds, ferments, goes out Bacterium, the process of concentration/concentrate drying.

Preparation method the most according to claim 3, it is characterised in that: described strain is At least one in glutamic acid bacillus, bacillus subtilis or corynebacterium ammoniagenes.

Preparation method the most according to claim 4, it is characterised in that: described fermentation Control condition is, inoculum concentration is 10 ± 1%, and temperature is 30-37 DEG C, and PH is 6.6-7.2, tank Pressure is 0.05MPa, dissolved oxygen >=15%.

Preparation method the most according to claim 3, it is characterised in that: described fermentation training The concentration proportioning supporting base percentage by weight is Semen Maydis starch 1.5-2%, sugared close 5-10%, di(2-ethylhexyl)phosphate Hydrogen potassium 0.1-0.3%, ammonium sulfate 0.2-0.5%, magnesium sulfate 0.1-0.3%, ferrous sulfate 0.03-0.05%, manganese sulfate 0.03-0.05%, glucose 5-10%, surplus is water.

Preparation method the most according to claim 3, it is characterised in that: described concentration/ Concentrate drying refers to concentrate the 3-8 times of paste obtained through negative pressure 50-90 DEG C after fermentation liquid sterilizing Product；Or continue to be dried to obtain powder product by creamy product again.

Preparation method the most according to claim 3, it is characterised in that: described fermentation Refer to that corynebacterium ammoniagenes fermentation reaches 3%-12.0% to the concentration of inosine monophosphate IMP；

Or Corynebacterium glutamicum/fermentation of bacillus subtilis reaches to fermentation liquid Glutamic Acid concentration 5%-17.0%；

Or the two mixture.