CN105906436A - Pinus thunbergii Parl rejuvenation substrate - Google Patents
Pinus thunbergii Parl rejuvenation substrate Download PDFInfo
- Publication number
- CN105906436A CN105906436A CN201610288001.XA CN201610288001A CN105906436A CN 105906436 A CN105906436 A CN 105906436A CN 201610288001 A CN201610288001 A CN 201610288001A CN 105906436 A CN105906436 A CN 105906436A
- Authority
- CN
- China
- Prior art keywords
- sulfate
- culture medium
- parts
- solution
- black pine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Inorganic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a Pinus thunbergii Parl rejuvenation substrate. The Pinus thunbergii Parl rejuvenation substrate is prepared from pitchstone, perlite, pine leaf soil, polyurethane, peanut shells, rice husks, vinasse, leaves, barks, EM (effective microorganisms), fruit peels, bran, bamboo leaves, calcium bicarbonate, complex enzymes, cortex albiziae, Lychnis coronata, peppers, Cortex Meliae, Changium smyrnioides, ferric sulfate, manganese sulfate, magnesium sulfate, calcium sulfate, zinc sulfate, sodium selenite, monopotassium phosphate, urea, potassium chloride, borax, a leavening agent and zeatin. The Pinus thunbergii Parl rejuvenation substrate has advantages that seedling growth can be promoted, seedling survival rate is increased, disease resistance and drought resistance of seedlings are improved, and high utilization rate is realized; a preparation method of the Pinus thunbergii Parl rejuvenation substrate is simple, easy in operation, low in investment and suitable for large-scale production and popularization.
Description
Technical field
The present invention relates to Pollen pini thunbergii technical field of cultivation, be specifically related to the culture medium of a kind of black pine seedlings.
Background technology
Pollen pini thunbergii (Pinus thunbergii Parl), calls white bud pine, aiphyllium, and high up to 30 meters, bark band is grey
Black, inhibited growth, moulding of entwining, attitude is magnificent, loud and sonorous magnificence.Pollen pini thunbergii is strong to adaptive capacity to environment, garden, balcony
Cultivating, the horizontal exhibition of its branch, tree crown such as canopy, needle is dark green, evergreen all the year round, and tree performance is quaint, can appreciate all the year round.Pollen pini thunbergii is not only basin
The outstanding plant planted, is also to use more outstanding nursery stock in afforestation, may be used for roadside greening, subdistrict greening, work
Factory afforests, square greening etc..
But, Pollen pini thunbergii cultivation most critical is the nursery of seedling, and owing to the seedling culture phase is the longest, investment is big and manages not
Kind so that nutritional need and the psychological need of seedling can not to be met at seedling culture period, directly result in seedling and send out survival rate
Low, resistance against diseases is poor, once meets with drought, flood, the meteorological disaster such as freezes, causes tree vigo(u)r weak, cause pest and disease damage to occur, and serious is straight
Connect and cause setting body death.
Summary of the invention
In order to solve the deficiencies in the prior art, and provide the culture medium of a kind of black pine seedlings, this black pine seedlings
Culture medium can improve survival rate and the resistance against diseases of black pine seedlings, preparation method is simple, easily operates, greatly reduces
Cost of investment.
The present invention is achieved through the following technical solutions:
The culture medium of a kind of black pine seedlings, is made up of the raw material of following weight portion: pitchstone 30-33 part, perlite 26-28
Part, Folium Pini soil 32-35 part, Pericarppium arachidis hypogaeae 16-18 part, rice husk 6-8 part, distiller grains 3-5 part, leaves 8-10 part, bark 7-8 part, EM bacterium
3-4 part, peel 6-8 part, wheat bran 14-15 part, Folium Bambusae 3-5 part, calcium bicarbonate 1-2 part, compound enzyme 1-1.8 part, Cortex Albiziae 0.5-
0.8 part, Herba lychnidis coronatae 0.7-0.8 part, Fructus Capsici 0.3-0.6 part, Cortex Meliae 0.5-0.7 part, Radix Changii 0.3-0.4 part, iron sulfate
0.2-0.3 part, manganese sulfate 0.2-0.4 part, magnesium sulfate 0.5-0.8 part, calcium sulfate 0.2-0.4 part, zinc sulfate 0.2-0.5 part, Asia
Sodium selenate 0.01-0.03 part, potassium dihydrogen phosphate 5-9 part, carbamide 10-14 part, potassium chloride 1-3 part, Borax 0.5-1 part, leaven
2-3 part, zeatin 2-2.6 part.
Described compound enzyme is made up of by weight following raw material: cellulase: protease: saccharifying enzyme=1:1:1.
The culture medium of described black pine seedlings is applicable to after presprouting of seeds to the seedling replanting stage.
The preparation method of the culture medium of described a kind of black pine seedlings, specifically includes following steps:
(1) prepared by fuel
A. by pitchstone, perlite, Folium Pini soil, pulverize, be ground into the powder of particle diameter 30-35 μm, put in 300 DEG C of high temperature
The expanded 2-3h of row, after natural cooling, obtains inorganic matrix material；
B. by Pericarppium arachidis hypogaeae, rice husk, distiller grains, leaves, bark co-grinding, crossing 80-85 mesh sieve, mixture moisture control exists
50-60%, adds EM bacterium, concentrates in heaps, and heap thickness is 50-55cm, cover film, ferments 12-15 days, obtains organic group material；
C. inorganic matrix material and organic group material are mixed, after banking up 5-7 days at ambient temperature 35-38 DEG C, turning 1 time,
Continue fermentation 7-9 days of banking up, obtain fuel, standby；
(2) preparation of nutritional solution
A. peel, wheat bran, Folium Bambusae are broken into the powder of particle diameter 120-140 μm, add calcium bicarbonate, add Semen Maydis, bean cake, bran
Skin and the pure water of calcium bicarbonate gross weight 1.4-1.6 times amount, mix and blend 6-8min, obtain solution A；
B. adding mass fraction in solution A is the compound enzyme of 0.08%, and pH is between 5.2-5.8 in regulation, enzyme at 40-45 DEG C
Solve 30min, obtain enzymolysis solution；
C., enzymolysis solution is warming up to 90-100 DEG C keeps 10-15min to carry out enzyme denaturing；
D, enzymolysis solution after enzyme denaturing is placed in supersonic frequency is 30-40KHz, extracts at ultrasonic temperature 40-50 DEG C, extracts 20-
30min, with 10000r/min, centrifugal 12-15min, take supernatant, obtain nutritional solution, standby；
(3) preparation of Chinese medicine extract
Cortex Albiziae, Herba lychnidis coronatae, Fructus Capsici, Cortex Meliae and Radix Changii are cleaned, pulverizes, cross 80-90 mesh sieve, obtain Chinese medicine powder A, in addition
The alcoholic solution that volume fraction is 30-35% of medicated powder A mass 3-3.3 times, big fire boils, and little fire infusion to cumulative volume is original
The 1/2 of volume, with 10000r/min, centrifugal 13min, take supernatant, obtain extracting solution, be placed in 45-47 DEG C and be concentrated into without moisture,
Chinese medicine extract, standby；
(4) preparation of culture medium
A, by iron sulfate, manganese sulfate, magnesium sulfate, calcium sulfate, zinc sulfate, sodium selenite, potassium dihydrogen phosphate, carbamide, potassium chloride and
Borax, after mix homogeneously, adds pure water, to being completely dissolved；
B, fuel, nutritional solution and Chinese medicine extract mixing, add leaven and zeatin, is placed in 28 DEG C and ferments 3-5 days, empty
Air humidity degree is 77-79%；
C, step A and step B are processed after raw material mixing, under room temperature, place 1-2 days, obtain the culture medium of black pine seedlings.
Beneficial effects of the present invention: a kind of culture medium promoting black pine seedlings that the present invention provides, utilization rate is high, preparation
Method is simple, easily operates, small investment, can carry out large-scale production and popularization；Containing inorganic matrix stone and organic group in fuel
Matter, the nutrient such as the calcium wherein contained, ferrum, manganese, zinc, magnesium, potassium, the nutritional need in seedling period can not only be met, also may be used
To improve the structure of soil, and then the healthy growth of promotion seedling, improve black pine seedlings and absorb nutrient and the energy of moisture；Addition is many
Planting Chinese crude drug, play antibacterial and antivirus action, reduce the pest and disease damage incidence rate of later stage seedling, sickness rate is by 40% original fall
Low is less than 3.8%, and disease resistance is obviously enhanced；In substrate, with the addition of zeatin, promote the growth of seedling, improve seedling
Survival rate, strengthen seedling disease-resistant, drought-resistant ability.
Detailed description of the invention
Below in conjunction with embodiment, the culture medium of black pine seedlings of the present invention is specifically described:
Embodiment 1
The culture medium of a kind of black pine seedlings, is made up of the raw material of following weight portion: pitchstone 30 parts, perlite 26 parts, Folium Pini
32 parts of soil, Pericarppium arachidis hypogaeae 16 parts, 8 parts of rice husk, 3 parts of distiller grains, leaves 8 parts, 7 parts of bark, EM bacterium 3 parts, 8 parts of peel, 15 parts of wheat bran, bamboo
5 parts of leaf, calcium bicarbonate 1 part, compound enzyme 1.4 parts, Cortex Albiziae 0.8 part, Herba lychnidis coronatae 0.7 part, 0.4 part of Fructus Capsici, Cortex Meliae 0.7 part,
Radix Changii 0.3 part, 0.3 part of iron sulfate, manganese sulfate 0.2 part, 0.6 part of magnesium sulfate, 0.4 part of calcium sulfate, 0.2 part of zinc sulfate, sub-selenium
Acid 0.02 part of sodium, potassium dihydrogen phosphate 6 parts, 11 parts of carbamide, 2 parts of potassium chloride, Borax 0.5 part, leaven 2 parts, zeatin 2.1 parts.
Described compound enzyme is made up of by weight following raw material: cellulase: protease: saccharifying enzyme=1:1:1.
The culture medium of described black pine seedlings is applicable to after presprouting of seeds to the seedling replanting stage.
The preparation method of the culture medium of described a kind of black pine seedlings, specifically includes following steps:
(1) prepared by fuel
A. by pitchstone, perlite, Folium Pini soil, pulverize, be ground into the powder of particle diameter 30-35 μm, put in 300 DEG C of high temperature
The expanded 2-3h of row, after natural cooling, obtains inorganic matrix material；
B. by Pericarppium arachidis hypogaeae, rice husk, distiller grains, leaves, bark co-grinding, crossing 80-85 mesh sieve, mixture moisture control exists
50-60%, adds EM bacterium, concentrates in heaps, and heap thickness is 50-55cm, cover film, ferments 12-15 days, obtains organic group material；
C. inorganic matrix material and organic group material are mixed, after banking up 5-7 days at ambient temperature 35-38 DEG C, turning 1 time,
Continue fermentation 7-9 days of banking up, obtain fuel, standby；
(2) preparation of nutritional solution
A. peel, wheat bran, Folium Bambusae are broken into the powder of particle diameter 120-140 μm, add calcium bicarbonate, add Semen Maydis, bean cake, bran
Skin and the pure water of calcium bicarbonate gross weight 1.4-1.6 times amount, mix and blend 6-8min, obtain solution A；
B. adding mass fraction in solution A is the compound enzyme of 0.08%, and pH is between 5.2-5.8 in regulation, enzyme at 40-45 DEG C
Solve 30min, obtain enzymolysis solution；
C., enzymolysis solution is warming up to 90-100 DEG C keeps 10-15min to carry out enzyme denaturing；
D, enzymolysis solution after enzyme denaturing is placed in supersonic frequency is 30-40KHz, extracts at ultrasonic temperature 40-50 DEG C, extracts 20-
30min, with 10000r/min, centrifugal 12-15min, take supernatant, obtain nutritional solution, standby；
(3) preparation of Chinese medicine extract
Cortex Albiziae, Herba lychnidis coronatae, Fructus Capsici, Cortex Meliae and Radix Changii are cleaned, pulverizes, cross 80-90 mesh sieve, obtain Chinese medicine powder A, in addition
The alcoholic solution that volume fraction is 30-35% of medicated powder A mass 3-3.3 times, big fire boils, and little fire infusion to cumulative volume is original
The 1/2 of volume, with 10000r/min, centrifugal 13min, take supernatant, obtain extracting solution, be placed in 45-47 DEG C and be concentrated into without moisture,
Chinese medicine extract, standby；
(4) preparation of culture medium
A, by iron sulfate, manganese sulfate, magnesium sulfate, calcium sulfate, zinc sulfate, sodium selenite, potassium dihydrogen phosphate, carbamide, potassium chloride and
Borax, after mix homogeneously, adds pure water, to being completely dissolved；
B, fuel, nutritional solution and Chinese medicine extract mixing, add leaven and zeatin, is placed in 28 DEG C and ferments 3-5 days, empty
Air humidity degree is 77-79%；
C, step A and step B are processed after raw material mixing, under room temperature, place 1-2 days, obtain the culture medium of black pine seedlings.
Embodiment 2
Embodiment 2 difference from Example 1 parts by weight of raw material in culture medium are different, and other are identical.
The culture medium of a kind of black pine seedlings, is made up of the raw material of following weight portion: pitchstone 32 parts, perlite 26 parts,
33 parts of Folium Pini soil, Pericarppium arachidis hypogaeae 16 parts, 6 parts of rice husk, 3 parts of distiller grains, leaves 9 parts, 7 parts of bark, EM bacterium 3 parts, 6 parts of peel, wheat bran 15
Part, 4 parts of Folium Bambusae, calcium bicarbonate 1 part, compound enzyme 1 part, Cortex Albiziae 0.5 part, Herba lychnidis coronatae 0.7 part, 0.3 part of Fructus Capsici, Cortex Meliae 0.7
Part, Radix Changii 0.4 part, 0.2 part of iron sulfate, manganese sulfate 0.4 part, 0.5 part of magnesium sulfate, 0.2 part of calcium sulfate, 0.2 part of zinc sulfate, Asia
Sodium selenate 0.03 part, potassium dihydrogen phosphate 6 parts, 11 parts of carbamide, 2 parts of potassium chloride, Borax 0.5 part, leaven 2 parts, zeatin 2.6
Part.
Embodiment 3
Embodiment 3 difference from Example 1 parts by weight of raw material in culture medium are different, and other are identical.
The culture medium of a kind of black pine seedlings, is made up of the raw material of following weight portion: pitchstone 33 parts, perlite 26 parts,
35 parts of Folium Pini soil, Pericarppium arachidis hypogaeae 18 parts, 7 parts of rice husk, 4 parts of distiller grains, leaves 9 parts, 7 parts of bark, EM bacterium 3 parts, 7 parts of peel, wheat bran 14
Part, 4 parts of Folium Bambusae, calcium bicarbonate 1 part, compound enzyme 1.6 parts, Cortex Albiziae 0.6 part, Herba lychnidis coronatae 0.7 part, 0.2 part of Fructus Capsici, Cortex Meliae
0.6 part, Radix Changii 0.4 part, 0.3 part of iron sulfate, manganese sulfate 0.4 part, 0.8 part of magnesium sulfate, 0.4 part of calcium sulfate, zinc sulfate 0.2
Part, sodium selenite 0.03 part, potassium dihydrogen phosphate 9 parts, 14 parts of carbamide, 2 parts of potassium chloride, Borax 0.6 part, leaven 2 parts, zeatin
2 parts.
Comparative example
The culture medium of a kind of black pine seedlings, is made up of the raw material of following weight portion: pitchstone 30 parts, perlite 26 parts, rice husk 8
Part, 3 parts of distiller grains, 7 parts of bark, EM bacterium 3 parts, potassium dihydrogen phosphate 6 parts, 11 parts of carbamide, 2 parts of potassium chloride.
The culture medium of described black pine seedlings is applicable to after presprouting of seeds to the seedling replanting stage.
The preparation method of the culture medium of described a kind of black pine seedlings, specifically includes following steps:
A. pitchstone, perlite are pulverized, be ground into the powder of particle diameter 30-35 μm, put in 300 DEG C of high temperature and carry out expanded 2-
3h, after natural cooling, obtains inorganic matrix material；
B. by rice husk, distiller grains, bark co-grinding, crossing 80-85 mesh sieve, mixture moisture control, at 50-60%, adds EM
Bacterium, concentrates in heaps, and heap thickness is 50-55cm, cover film, ferments 12-15 days, obtains organic group material；
C. inorganic matrix material and organic group material are mixed, after banking up 5-7 days at ambient temperature 35-38 DEG C, turning 1 time, continues
Continuous fermentation 7-9 days of banking up, obtains fuel, standby；
D. by fuel adds potassium dihydrogen phosphate, carbamide, potassium chloride, uniformly mix, obtain the culture medium of black pine seedlings.
In areal, the culture medium of identical culture medium, embodiment and comparative example is used to cultivate seedling 1000 respectively,
Statistics Pollen pini thunbergii pine big aphid insect damage rate, die-back disease rate, the actual state of survival rate respectively, embodiment and the cultivation base of ratio battalion
The upgrowth situation of matter black pine seedlings is shown in Table 1.
Table 1: the black pine seedlings upgrowth situation of the culture medium of embodiment and ratio battalion
| Project | Big aphid insect damage rate/(%) of pine | Die-back disease rate/(%) | Survival rate/(%) |
| Embodiment 1 | 5.9 | 4.8 | 94.2 |
| Embodiment 2 | 6.1 | 5.1 | 94.7 |
| Embodiment 3 | 5.3 | 5.0 | 94.2 |
| Comparative example | 12.8 | 11.3 | 76.7 |
As it can be seen from table 1 embodiment promotes the culture medium of black pine seedlings growth, the pest and disease damage rate of black pine seedlings is aobvious relatively to be contrasted
Example is low, and survival rate, apparently higher than comparative example, illustrates that the culture medium that the promotion black pine seedlings that the present invention provides grows can be effective
Strengthen resistance against diseases, improve the survival rate of black pine seedlings.
Claims (4)
1. the culture medium of a black pine seedlings, it is characterised in that be made up of the raw material of following weight portion: pitchstone 30-33 part,
Perlite 26-28 part, Folium Pini soil 32-35 part, Pericarppium arachidis hypogaeae 16-18 part, rice husk 6-8 part, distiller grains 3-5 part, leaves 8-10 part, bark
7-8 part, EM bacterium 3-4 part, peel 6-8 part, wheat bran 14-15 part, Folium Bambusae 3-5 part, calcium bicarbonate 1-2 part, compound enzyme 1-1.8 part,
Cortex Albiziae 0.5-0.8 part, Herba lychnidis coronatae 0.7-0.8 part, Fructus Capsici 0.3-0.6 part, Cortex Meliae 0.5-0.7 part, Radix Changii 0.3-0.4
Part, iron sulfate 0.2-0.3 part, manganese sulfate 0.2-0.4 part, magnesium sulfate 0.5-0.8 part, calcium sulfate 0.2-0.4 part, zinc sulfate 0.2-
0.5 part, sodium selenite 0.01-0.03 part, potassium dihydrogen phosphate 5-9 part, carbamide 10-14 part, potassium chloride 1-3 part, Borax 0.5-1
Part, leaven 2-3 part, zeatin 2-2.6 part.
The culture medium of a kind of black pine seedlings the most according to claim 1, it is characterised in that described compound enzyme is by following
Raw material is by weight composition: cellulase: protease: saccharifying enzyme=1:1:1.
The culture medium of a kind of black pine seedlings the most according to claim 1, it is characterised in that the training of described black pine seedlings
Educate substrate to be applicable to after presprouting of seeds to the seedling replanting stage.
The preparation method of the culture medium of a kind of black pine seedlings the most according to claim 1, it is characterised in that specifically include
Following steps:
(1) prepared by fuel
A. by pitchstone, perlite, Folium Pini soil, pulverize, be ground into the powder of particle diameter 30-35 μm, put in 300 DEG C of high temperature
The expanded 2-3h of row, after natural cooling, obtains inorganic matrix material；
B. by Pericarppium arachidis hypogaeae, rice husk, distiller grains, leaves, bark co-grinding, crossing 80-85 mesh sieve, mixture moisture control exists
50-60%, adds EM bacterium, concentrates in heaps, and heap thickness is 50-55cm, cover film, ferments 12-15 days, obtains organic group material；
C. inorganic matrix material and organic group material are mixed, after banking up 5-7 days at ambient temperature 35-38 DEG C, turning 1 time,
Continue fermentation 7-9 days of banking up, obtain fuel, standby；
(2) preparation of nutritional solution
A. peel, wheat bran, Folium Bambusae are broken into the powder of particle diameter 120-140 μm, add calcium bicarbonate, add Semen Maydis, bean cake, bran
Skin and the pure water of calcium bicarbonate gross weight 1.4-1.6 times amount, mix and blend 6-8min, obtain solution A；
B. adding mass fraction in solution A is the compound enzyme of 0.08%, and pH is between 5.2-5.8 in regulation, enzyme at 40-45 DEG C
Solve 30min, obtain enzymolysis solution；
C., enzymolysis solution is warming up to 90-100 DEG C keeps 10-15min to carry out enzyme denaturing；
D, enzymolysis solution after enzyme denaturing is placed in supersonic frequency is 30-40KHz, extracts at ultrasonic temperature 40-50 DEG C, extracts 20-
30min, with 10000r/min, centrifugal 12-15min, take supernatant, obtain nutritional solution, standby；
(3) preparation of Chinese medicine extract
Cortex Albiziae, Herba lychnidis coronatae, Fructus Capsici, Cortex Meliae and Radix Changii are cleaned, pulverizes, cross 80-90 mesh sieve, obtain Chinese medicine powder A, in addition
The alcoholic solution that volume fraction is 30-35% of medicated powder A mass 3-3.3 times, big fire boils, and little fire infusion to cumulative volume is original
The 1/2 of volume, with 10000r/min, centrifugal 13min, take supernatant, obtain extracting solution, be placed in 45-47 DEG C and be concentrated into without moisture,
Chinese medicine extract, standby；
(4) preparation of culture medium
A, by iron sulfate, manganese sulfate, magnesium sulfate, calcium sulfate, zinc sulfate, sodium selenite, potassium dihydrogen phosphate, carbamide, potassium chloride and
Borax, after mix homogeneously, adds pure water, to being completely dissolved；
B, fuel, nutritional solution and Chinese medicine extract mixing, add leaven and zeatin, is placed in 28 DEG C and ferments 3-5 days, empty
Air humidity degree is 77-79%；
C, step A and step B are processed after raw material mixing, under room temperature, place 1-2 days, obtain the culture medium of black pine seedlings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610288001.XA CN105906436A (en) | 2016-05-04 | 2016-05-04 | Pinus thunbergii Parl rejuvenation substrate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610288001.XA CN105906436A (en) | 2016-05-04 | 2016-05-04 | Pinus thunbergii Parl rejuvenation substrate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105906436A true CN105906436A (en) | 2016-08-31 |
Family
ID=56753557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610288001.XA Pending CN105906436A (en) | 2016-05-04 | 2016-05-04 | Pinus thunbergii Parl rejuvenation substrate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105906436A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106478189A (en) * | 2016-10-27 | 2017-03-08 | 六安市振清生态农业有限公司 | Nutrition organic substrate is used in a kind of Pollen pini thunbergii plantation |
| CN109463248A (en) * | 2018-12-29 | 2019-03-15 | 青岛冠中生态股份有限公司 | A kind of sand ground black pine tree transplanted method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104737851A (en) * | 2015-04-21 | 2015-07-01 | 赖永梅 | Black pine rejuvenation matrix |
-
2016
- 2016-05-04 CN CN201610288001.XA patent/CN105906436A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104737851A (en) * | 2015-04-21 | 2015-07-01 | 赖永梅 | Black pine rejuvenation matrix |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106478189A (en) * | 2016-10-27 | 2017-03-08 | 六安市振清生态农业有限公司 | Nutrition organic substrate is used in a kind of Pollen pini thunbergii plantation |
| CN109463248A (en) * | 2018-12-29 | 2019-03-15 | 青岛冠中生态股份有限公司 | A kind of sand ground black pine tree transplanted method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103553839B (en) | Special compound fertilizer for corn and preparation method thereof | |
| CN105130690A (en) | Chili-controlled release fertilizer and preparation method thereof | |
| CN105272741A (en) | Biological organic pesticide-containing fertilizer and preparation method thereof | |
| CN104761391B (en) | A kind of Chinese torreya seedling Special base fertilizer and preparation method thereof | |
| CN104761375A (en) | Seaweed biological fertilizer and preparation method thereof | |
| CN105399567A (en) | Henry steudnera tuber controlled-release fertilizer, and preparation method thereof | |
| CN105237138A (en) | Release-controlled fertilizer for Chinese yam and preparation method thereof | |
| CN104885761A (en) | Cultivation method of conservation rock-desertification area sechium edule | |
| CN105198591A (en) | Leaf mustard controlled-release fertilizer and preparation method thereof | |
| CN105198604A (en) | Fragrant taro controlled-release fertilizer and preparation method thereof | |
| CN107896755A (en) | A kind of method for integrated control of hickory nut dry rot | |
| CN104885857A (en) | Method for cultivating chayote in Karst rocky desertification mountainous regions | |
| CN106278762A (en) | Dahlia Pinnata Cav. compound fertilizer | |
| CN105493942A (en) | Nutrient soil for Adinandra milletii seedling culture | |
| CN105906436A (en) | Pinus thunbergii Parl rejuvenation substrate | |
| CN104230404A (en) | Preparation method of enzymatic hydrolysis algae organic fertilizer and product thereof | |
| CN105198588A (en) | Tea tree controlled-release fertilizer and preparation method thereof | |
| CN106348916A (en) | Special biological fertilizer for moso bamboo with drought resistance and moisture retention effects and production method thereof | |
| CN105967900A (en) | Pest-resistant nutrient solution for indoors cultivated flowers and plants and preparation method thereof | |
| CN104472299A (en) | Cultivation method of papayas in sunlight greenhouse | |
| CN108432539A (en) | A kind of Chinese prickly ash implantation methods rich in various trace elements | |
| CN107960220A (en) | A kind of arborization cultural method of Chinese tamarisk | |
| CN104447091B (en) | Special leaf fertilizer for kuding tea and production method thereof | |
| CN108503412A (en) | A kind of ecological efficient organic type tomato seedling substrate | |
| CN105198592A (en) | Banana controlled-release fertilizer and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160831 |
|
| RJ01 | Rejection of invention patent application after publication |