CN105902476A - Moisture retention and regeneration plant extract composition and its use in cosmetic - Google Patents
Moisture retention and regeneration plant extract composition and its use in cosmetic Download PDFInfo
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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Abstract
The invention discloses a moisture retention and regeneration plant extract composition. The moisture retention and regeneration plant extract composition comprises, by weight, 15-30 parts of camellia seed extract, 10-20 parts of aloe vera extract, 8-15 parts of oat extract, 10-15 parts of simmondsia chinensis seed extract, 5-10 parts of Centella asiatica extract, 2-5 parts of grape seed extract, 5-8 parts of ferment powder and 2-5 parts of lactobacillus plantarum powder. The camellia seed extract, oat extract, simmondsia chinensis seed extract and Centella asiatica extract can tighten a connection part of epidermis and dermis, soften skin, promote corium layer collagen formation and tighten skin. The ferment powder contains lactic acid and vegetable water-soluble nutrients such as vitamins and lactic acid bacteria. On a mask, lactic acid bacteria can produce lactic acid through metabolism.
Description
Technical field: the invention belongs to cosmetic field, particularly to moisture saver mask product.
Background technology
The fundamental of healthy skin is smooth, full, high resilience.Increase along with the age; Moisture factor content in keratodermatitis gradually decreases; the hydratability causing skin declines; show as xerosis cutis, wrinkling, lax; present aging outward appearance; the most current atmosphere protection layer is destroyed; ultraviolet radiation is serious; the environmental pollutions such as dust, haze, sandstorm, PM2.5, computer radiate, second hand smoking, vehicle exhaust, industrial pollution, waste gas, all can cause xerosis cutis, stimulation, allergy, skin hydropenia, sensitivity, fat secretion is unbalance, aging, the problem that also can make blackhead, corse sweat pore.In addition, urban life pressure is big, the most tired, stay up late, with advancing age, causes xerosis cutis to relax, and tarnishes, skin resistance declines, and is most susceptible to external invasion and attack, causes allergy, dimness, lax dry skin.
Facial film is a kind of cosmetics that people seeking beauty commonly uses, and facial film is of a great variety, and effect is the most different.According to the difference of making raw material, moisturizing, whitening, speckle dispelling and other functional masks can be divided into.As skin of releiving, strengthen skin metabolic detoxification effect, get rid of skin toxin, improve skin quality, use after the colour of skin become the brightest glossy, improve the colour of skin wax yellow, obscure, be dried puzzlement etc..Can also strengthen or increase other effects, such as elastic force moisturizing, lifting of compacting, skin-moisturizing, skin, whitening despeckle, wrinkle resistant defying age, antiinflammatory toxin expelling, preventing and treating skin sore etc..Initial stage facial film uses synthetic as base stock mostly, and along with the improvement of functional additive in formula, purer extraction, mask product more natural, safer, more effective have been had by consumer further to be pursued.
The Chinese patent of Publication No. CN 104352421 A discloses " a kind of skin rejuvenating facial mask liquid ", the skin rejuvenating facial mask liquid of this invention, its composition includes 1 by mass percentage, 3 butanediols 2.5%～3.5%, HANSHENGJIAO 0.15%～0.25%, disodiumedetate 0.04%～0.06%, glycerol 4%～6%, glycyrrhizic acid dipotassium 0.05%～1.2%, 1, 2 pentanediols 1.5%～3.5%, hyaluronate sodium 0.03%～0.2%, Aloe Barbadensis Miller juice 3%～35%, tremella extract 1%～15%, Herba Portulacae extract 0.5%～6%, β glucose 0.4%～7%, Flos Matricariae chamomillae extracting solution 0.5%～6%, Mel 0.5%～6%, chlorphenesin 0.1%～0.25% etc..The present invention compared with prior art, has the advantages such as defying age, crease-resistant, skin care, the most also can repair sun-damaged skin, the advantages such as sensitivity of releiving.
The patent of invention of Publication No. CN 105106064 A discloses " a kind of moisturizing Wash-free mask and preparation method thereof ", and it is made up of solid grease, liquid fat, emulsifying agent, rheology control agent, wetting agent, plant extract and deionized water；It addition, present invention also offers the preparation method of described facial film.The made facial film of the present invention is compared with prior art; products made thereby moistens gentleness especially; safety is high; the compatibility and compatibility between oils and fats are preferable; utilize the high moistening effect collocation polyalcohols wetting agent of oat beta-glucan; moistening effect is splendid; the sensitive skin of neutral red bisabolol protection, adds Herba Centellae extract, promotes wound healing and the effect of anti-inflammation; flexible with rear skin; moistening gloss, feel to skin silk smooth felling, the advantage of its maximum is without film material; without excessive packaging, reduce the wasting of resources and beneficially sustainable development.
Publication No. CN 104814918 A disclosure of the invention " a kind of replenishing water and preserving moisture facial mask liquid and manufacture method thereof ", by weight percentage, it is made up of following components: polymer hyaluronic acid sodium: 0.1-2.0%, oligomerization hyaluronate sodium: 0.1-2%, disodiumedetate (EDTA-2Na): 0.01-0.2%, anti-sensitizer: 0.01-5.0%, the first wetting agent: 1-8%, preservative: 0.01-0.5%, the second wetting agent: 1-6% lactobacillus/Sucus Pyri tunning filtrate: 0.5-2% deionized water: its surplus.The facial mask liquid of the present invention, preparation method is simple, utilizes " dual Moisture factor " polymer hyaluronic acid sodium and oligomerization hyaluronate sodium, and " biotic factor " lactobacillus.
Chinese patent: the preparation method of bioanalysis rapid fermentation Pickles, Patent No. 201110421967.3；Disclose the preparation method of bioanalysis rapid fermentation Pickles, the invention belongs to field of vegetable deep-processing, particularly to the method utilizing composite bacterium powder to produce Pickles.The present invention solves the technical problem quickly producing high-quality fermentation pickled vegetable.Key step of the present invention has: (1) charging seals: put into after vegetable raw-material cutting in container, adds containing acetobacter, the composite bacterium powder of saccharomyces cerevisiae and the adjuvant containing Sal, seals container；(2) fermentation: controlling temperature and carry out prior fermentation at 23-36 DEG C, control temperature subsequently to carry out after fermentation at 15-25 DEG C, whole fermentation time was at 20-40 hour；(3) dehydration allotment: ferment complete Pickles moisture of sloughing, adds flavoring for mixture uniformly.The present invention is applicable to the pickle production of different scales, can substantially shorten the production time, and kimchi products is nutritious, pure taste, and this product has broad application prospects in pickle production field.
" preparation method of the prebiotic Pickles of bioanalysis rapid fermentation; application number: 201210310308.7 applyings date: the invention discloses the preparation method of the prebiotic Pickles of bioanalysis rapid fermentation; the invention belongs to field of vegetable deep-processing, particularly to the method utilizing composite bacterium powder to produce Pickles for Chinese patent.The present invention solves the technical problem quickly producing high-quality fermentation pickled vegetable.Key step of the present invention has: (1) charging seals: put into after vegetable raw-material cutting in container, adds containing acetobacter, the composite bacterium powder of saccharomyces cerevisiae and the adjuvant containing Sal, seals container；(2) fermentation: controlling temperature and carry out prior fermentation at 23-36 DEG C, control temperature subsequently to carry out after fermentation at 15-25 DEG C, whole fermentation time was at 20-40 hour；(3) dehydration allotment: ferment complete Pickles moisture of sloughing, adds flavoring for mixture uniformly.The present invention is applicable to the pickle production of different scales, can substantially shorten the production time, and kimchi products is nutritious, pure taste, and this product has broad application prospects in pickle production field.
The patent of invention of Publication No. CN 103976913A discloses " a kind of face mask of traditional Chinese medicine for moisturizing ", medicine including following parts by weight is prepared from: the Rhizoma Chuanxiong of 20-30 parts by weight, the Aloe of 20-30 parts by weight, the Fructus Momordicae of 15-25 parts by weight, the Radix Asparagi of 15-25 parts by weight, the Sargassum of 35-45 parts by weight, the Flos Lonicerae of 15-25 parts by weight, the Radix Glycyrrhizae of 5-15 parts by weight.This invention have invigorate blood circulation moisturize, clearing heat and moistening lung, skin moistening moisturizing, the effect of dry moisturizing of dispelling.
Although current facial film miscellaneous is innumerable, but the replenishing water and preserving moisture effect of moisture saver mask is the most not fully up to expectations, and safety is unstable, could not fundamentally improve skin problem etc., therefore, it is necessary to research and develop a kind of natural safety, moisturizing, the lock good moisture saver mask liquid of water effect.
Summary of the invention:
The present invention provides a kind of moisturizing, rehabilitation plant extractive composition and the application in cosmetics thereof:
Moisturizing, the parts by weight of rehabilitation plant extractive composition form as follows:
Camellia extract 15-30, aloe vera extract 10-20, oat extract 8-15, Jojoba seed extract 10-15,
Herba Centellae extract 5-10, Semen Vitis viniferae extract 2-5, ferment powder 5-8, Lactobacillus plantarum mycopowder 2-5.
The preparation method of described Herba Centellae extract comprises the steps: to pulverize Herba Centellae to be placed on equipped with in 0.3-0.5% sodium bicarbonate solution, it is 7-8 with breast acid for adjusting pH value, it is warming up to 40-50 DEG C, add the mixed enzyme of Herba Centellae quality 1-2%, stir, enzymolysis 35-50min, subsequently in electric field intensity 20-40kV/cm, burst length 400-500 μ s, carry out high voltage electric field under the conditions of pulse frequency 200-300Hz to process 5-10 minute, then room temperature 400W, 40KHz condition supersound process 10-15min；The enzymolysis solution of above-mentioned process is drying to obtain Herba Centellae extract through 100-300 eye mesh screen filter freezing；The quality group of described mixed enzyme becomes: saccharifying enzyme: pectase: cellulase=1:2-5:5-10.
The preparation method of described Jojoba seed extract comprises the following steps: Jojoba seed adds the water of its quality 3-7 times after pulverizing, in electric field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high voltage electric field and extracts 6-10 minute under the conditions of pulse frequency 200-300Hz；It is 7-9 with sodium carbonate regulation pH value, adds the compound enzyme of Jojoba seed quality 1-2%, in 48-55 DEG C of enzymolysis 50-80min；Enzymolysis solution lyophilization, low-temperature grinding to particle diameter are that 0.1-0.3mm i.e. obtains Jojoba seed extract；Described compound enzyme is that cellulase, xylanase, laccase, pectase 6:5-10:2:1 in mass ratio uniformly mixes.
The preparation method of described oat extract, comprise the steps: by after Herba bromi japonici surface sprinkling 2-5% lactic acid solution in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, particle diameter is 0.1-1mm；Add the deionized water of ground product quality 3-5 times, mix homogeneously, it is 5-7 with Fructus Citri Limoniae acid for adjusting pH value, add the mixed enzyme of Herba bromi japonici quality 3-5%, stir, adjust temperature and be 65-70 DEG C, enzymolysis 50-80min, enzymolysis solution filters through 100-300 eye mesh screen, and freeze-dried i.e. the obtaining of filtrate i.e. obtains oat extract.The quality group of described mixed enzyme becomes: saccharifying enzyme: pectase: cellulase=1:1-2:6-10.
Described Camellia method for preparing extractive is: Camellia is crushed to particle diameter 0.1-0.3mm, add the water of Camellia quality 1-3 times, it is 5-6 with breast acid for adjusting pH value, it is warming up to 40-50 DEG C, add the mixed enzyme of Camellia quality 3-5%, stir, enzymolysis 55-80min, enzymolysis solution filters through 100-300 eye mesh screen, and filtrate is freeze-dried i.e. obtains Camellia extract；The quality group of described mixed enzyme becomes: pectase: cellulase=1:6-10.
Aloe vera extract preparation method is as follows: take Aloe vulgaris in-18-23 DEG C of freezing 5-10min；It is crushed to particle diameter 0.3-1mm immediately；Put in container and add 2-3% sodium carbonate liquor regulation pH value be 7-9, be warming up to 60-70 DEG C, insulation, be subsequently adding the mixed enzyme enzymolysis 30-50min of mixed material gross weight 2-5%, filter, obtain filtrate；Filtrate is through ultrafiltration lyophilization and get final product.Described mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:8-15:2 in mass ratio.
Semen Vitis viniferae extract preparation method is as follows: take after Semen Vitis viniferae surface sprinkling lactic acid in-18-23 DEG C of freezing 30-50min；It is crushed to particle diameter 0.3-1mm immediately；Put in container and add 2-3% sodium carbonate liquor regulation pH value be 7-9, be warming up to 60-70 DEG C, insulation, be subsequently adding the mixed enzyme enzymolysis 30-50min of mixed material gross weight 2-5%, filter, obtain filtrate；Filtrate is through ultrafiltration postlyophilization and get final product.Described mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:8-15:2 in mass ratio.
Ferment powder preparation method is as follows:
The big grain materials such as the fermentation liquid using pickle fermentation to produce discharge in latter stage is raw material, the dish leaf being filtered to remove in fermentation liquid obtain fermented liquid, and fermented liquid uses the drying meanss such as lyophilization to prepare ferment powder.
Cryodesiccated condition is :-30-40 degree pre-freeze 6 hours；Then lyophilizing is until water content is less than 5%.Pickle production method is prepared with reference to Chinese patent 201110421967.3 or 201210310308.7.
The preparation method of Lactobacillus plantarum mycopowder is as follows:
Lactobacillus plantarum powdery mycopowder, production stage is as follows: slant strains is transferred to fluid medium the volume required that spreads cultivation step by step；The bacterium solution obtained spreading cultivation is centrifuged separating, and collects precipitation thalline；In precipitation thalline, add protective agent and be diluted；Utilizing drying equipment to prepare powdery microbial inoculum, it is (0.1～9.0) * (10 that said method prepares viable count in Lactobacillus plantarum mycopowder10) individual/gram, starch can be added during mycopowder mixing and dextrin realizes the proper ratio of each bacterium viable count.Described Lactobacillus plantarum is CGMCC 11763.
One of facial film production method:
A phase raw material composition and mass ratio (mass percent):
Sodium acrylate/sodium acryloyldimethyl taurate copolymers 0.5-0.8,2-Methylpentadecane 2, polydimethylsiloxane 0.5-0.8, caprylic/capric triglyceride 1.5-2, polysorbate-20 0.5-0.8.
B phase raw material forms: glycerol 2, methyl hydroxybenzoate 0.1-0.2, carbomer 0.07-0.1, allantoin 0.1-0.2, glycine betaine 2, hyaluronic acid 0.01-0.05, glycyrrhizic acid dipotassium 0.1-0.3, EDETATE SODIUM 0.01-0.05
C phase raw material forms: triethanolamine 0.07-0.1,
D phase raw material forms: polydimethylsiloxane 0.4-0.6, Methylisothiazolinone 0.1-0.2, essence 0.05-0.08.
Remaining is water.
Production method is as follows:
1. adding water to be slowly added under aqueous phase pot, high-speed stirred raw material carbomer, heated and stirred is to being completely dissolved；
2.2. it is sequentially added into the B whole component of phase raw material, is heated with stirring to be completely dissolved, and be warming up to 85 DEG C of insulations 30 minutes；
3.3. will aqueous phase suction emulsifying pot cool；
4.4. 70 DEG C time add be pre-mixed uniform A phase component, high speed homogenization stops homogenizing after 5 minutes, homogenization condition is:；
5.5. insulated and stirred cooled down after 10 minutes；
6.6. 45 DEG C time add C phase and D phase raw material, stir the plant extract combination agent adding 15-30% to mix homogeneously.
7. 38 DEG C of dischargings or coat non-woven fabrics and be prepared as mask product.
Beneficial effect:
Camellia extract: oat extract:, Jojoba seed extract: Herba Centellae can be compacted epidermis and corium coupling part, skin can be made to limber up, help to promote that in skin corium, collagen protein is formed, there is the effect making skin compact, containing lactic acid in ferment powder, vegetable water solublity nutritional labeling vitamin, lactic acid bacteria, on facial film, lactic acid bacteria metabolism can produce lactic acid.Water replenishment and intensive care, allow skin obtain and purely loosen and beautiful enjoyment.Filled moisture, the skin fully soaked into, burst forth smooth tender and bright gloss, and soft and moist careful care helps skin to resist external and internal pressure.Extraction, from beautiful graceful Flos Camelliae Japonicae, contains remarkable moisturizing energy, it is possible to helps reduction skin and makes it keep preferable water profit state.There is maintenance effect, it is possible to protection skin is from infringement, and the oneself strengthening skin resists mechanism.
Specific embodiment
Described Lactobacillus plantarum is CGMCC 11763.
Lactobacillus plantarum CGMCC NO.11763 provided by the present invention is found under conditions of pH is 1.50 survive, still in existing state after 1% cholate is cultivated 4 hours through experiment；Lactobacillus plantarum CGMCC NO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg；CGMCC NO.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol.CGMCC NO.11763 Adhering capacity measure from coagulation rate be 95.71%.
CGMCC NO.11763 bacterial strain is to cholesterol degradation capability study and mensuration:
Take 1ml CGMCC NO.11763 mother solution and be inoculated in MRS cholesterol fluid medium (the cholesterol level 0.1mg/ml of 10mL, pH 6.2) in, the constant temperature of 37 DEG C stands and cultivates 20h respectively, 40h, 60h is standby, with access 1mL sterilized water MRS cholesterol culture medium for comparison, take bacteria liquid sample and the comparison each 1ml of liquid of above cultivation different time, 9000r/min, centrifugal 10min at 4 DEG C, obtain fermented supernatant fluid, in o-phthalaldehyde method mensuration supernatant, cholesterol level is (particularly as follows: take each supernatant 0.1ml in corresponding test tube, add glacial acetic acid 0.3ml, o-phthalaldehyde(OPA) 0.15ml of 1mg/ml, it is slowly added into concentrated sulphuric acid 1.0ml, mix homogeneously.Room temperature stands 10min, surveys light absorption value under 550nm).Each process 3 repetition, in kind makes cholesterol standard curve, calculates cholesterol level and degradation rate in supernatant, and result is shown inTable 1.Understanding, CGMCC NO.11763 has good Degradation to cholesterol, and after fermentation 60h hour, degradation rate can reach 64.76%.
Table 1Degraded situation to cholesterol.
| Degradation time (h) | 0 | 20h | 40h | 60h |
| Cholesterol level (mg/ml) | 0.2273±0.0058 | 0.1356±0.0018 | 0.1011±0.0094 | 0.801±0.0231 |
| Degrading rate of cholesterol % | 40.34% | 55.52% | 64.76% |
The bile tolerance test of CGMCC NO.11763 bacterial strain:
Take CGMCC NO.11763 bacterium solution 1mL inoculation strain in 10mL MRS fluid medium (PH=6.4) containing different cholate (concentration gradients is 0.0%, 0.2%, 0.4%, 0.6%, 0.8%, 1%), be placed at 37 DEG C and cultivate 0 respectively, 2,4h, each process 3 repetition.Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilution factor solution, take 0.1ml diluent to be coated with in MRS, be inverted in 37 DEG C of biochemical cultivation cases and cultivate 48 hours (each dilution factor do 3 parallel) record and calculate the several number of bacterium on flat board.Result is shown inTable 2.Understand this bacterium increment of bacterium after gallbladder salinity is 1% process 4h and still reach 0.59 ± 0.92 × 107(cfu/ml), there is good bile tolerance ability.
Table 2Bile tolerance ability detection [(± s) × 107cfu/ml]
The acid resistance test of CGMCC NO.11763 bacterial strain
Take CGMCC NO.11763 mother solution by 1ml inoculation strain in the 10mL MRS fluid medium of different pH value (pH gradient is 1.5,2.0,2.5,3.0,3.5,4.0), be placed at 37 DEG C and cultivate 0 respectively, 2,4h, each process 3 repetition.Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilute solution, take 0.1ml diluent and be coated with in MRS, in 37 DEG C of biochemical cultivation cases, be inverted the bacterium colony number cultivated on 48 hours (each dilution factor do 3 parallel) record flat board.Result is shown inTable 3.Illustrate that this bacterium has the strongest acid-fast ability.
Table 3Acid-fast ability detection [(± s) × 107cfu/ml]
The Adhering capacity of CGMCC NO.11763 bacterial strain measures
Cultivate CGMCC NO.11763 (MRS fluid medium), escherichia coli DH 5 α (LB fluid medium) 24h obtains fermentation liquid, it is respectively placed in 3000r/min, centrifugal 10min at 4 DEG C, collect bacterium mud, (in bacterium colony, PBS is i.e. added 2 times respectively with sterile phosphate buffer (PBS) the washing bacterium mud of pH=7.0, after concussion mix homogeneously, it is placed in 3000r/min, centrifugal 10min at 4 DEG C, collects thalline).From coagulation rate (%): with aseptic PBS, bacterium mud CGMCC NO.11763 is formed in suspension bacteria liquid and the bacteria suspension that the light absorption value at wavelength 600nm is 0.4 ± 0.1 (A 0), measure light absorption value A 24 after standing 24h, be (A 0-A 24)/A 0 from coagulation rate (%) formula.；His coagulation rate (%): the outstanding bacterium solution of CGMCC NO.11763 and bacillus coli DH 5 alpha is adjusted to the mix suspending bacterium solution that the light absorption value at wavelength 600nm is 0.6 ± 0.1 (A 0).Measuring light absorption value A 24 after standing 24H, his coagulation rate (%) formula is (A 0-A 24)/A 0.Measurement result is shown in Table 4, it is known that CGMCC NO.11763 is 95.71% from coagulation rate, has the strongest Adhering capacity.
Table 4Adhering capacityTable
Described Lactobacillus plantarum (Lactobacillus plantarum) XH was on November 30th, 2015PreservationIn Chinese microorganism strainPreservationManagementCommitteeCGMCC (is called for short) in common micro-organisms center,PreservationNumber it is CGMCC NO.11763,PreservationAddress is: inState northNorth Star West Road, Jing Shi Chaoyang District 1 institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.
This bacterial strain feature is as follows: examine under a microscope, and this bacterial strain is rod-short, and Gram’s staining is positive, atrichia, does not produce spore；On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.
Physicochemical characteristics is: catalase (-),GelatinLiquefaction (-), indole experiment (+), mobility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), product hydrogen sulfide gas (-), pH4.0MRS culture medium grows (+).It is accredited as Lactobacillus plantarum (Lactobacillus plantarum), named Lactobacillus plantarum (Lactobacillus plantarum) XH through Physiology and biochemistry.
This bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.
Lactobacillus plantarum of the present invention is by gatheringPeople LeeContribute, fromXinjiangIsolated in Yoghourt in fellow-villager family of the Uygur nationality, acquisition time on June 2nd, 2015.
5L fermentation tank is tested
(1) Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane is taken, access in the 250mL triangular flask equipped with 50mL culture medium MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm, cultivate about 12h, make thalline be in mid log phase for 37 DEG C.
(2) strain of logarithmic (log) phase is accessed in the 5L fermentation tank equipped with 3L MRS fluid medium (initial glucose is 150g/L).Inoculum concentration is 10%, and at 37 DEG C, 100rpm cultivates 8 hours, and logarithm early stage dissolved oxygen controls 10% (ventilation 0.5L/min), later stage Anaerobic culturel 63 hours.After fermentation ends, the lactic acid production of Lactobacillus plantarum CGMCC NO.11763 reaches 110g/L.Such lactic acid producing speed is beneficial to the rapid fermentation of Pickles.
(4) strain of logarithmic (log) phase is accessed in the 5L fermentation tank equipped with the 3L sodium nitrite liquid screening medium modified MRS screening culture medium of 2g/L sodium nitrite (the single nitrogen source be).Inoculum concentration is 10%, and at 37 DEG C, 100rpm cultivates 8 hours, and logarithm early stage dissolved oxygen controls 10% (ventilation 0.5L/min), and later stage anaerobism, sweat adds the sodium nitrite solution of 20g/L according to the wear rate stream of nitrite, cultivates 2-3 days.After fermentation ends, calculate the sweat Lactobacillus plantarum CGMCC NO.11763 degradation rate to sodium nitrite.Found that: under this condition, XH can reach 653mg/h/L to the degradation rate of sodium nitrite.
(5) the strain 10mL of logarithmic (log) phase is accessed equipped with in Chinese cabbage pretreated for 2kg, be processed according to conventional Kimchi method, the content of nitrite measured in Pickles for every 12 hours.It was found that in whole sweat, XH bacterium is 10.9mg/h/kg Chinese cabbage to the decomposition rate of sodium nitrite.Content of sodium nitrite in Pickles is consistently lower than 4.8mg/kg, is far belowCountryThe content (20mg/kg) of regulation in standard GB2714-2003.
Embodiment 1
The present invention provides a kind of moisturizing, rehabilitation plant extractive composition and the application in cosmetics thereof:
Moisturizing, the parts by weight of rehabilitation plant extractive composition form as follows:
Camellia extract 20, aloe vera extract 15, oat extract 10, Jojoba seed extract 12, Herba Centellae carries
Take thing 8, Semen Vitis viniferae extract 3, ferment powder 6, Lactobacillus plantarum mycopowder 3.
The preparation method of described Herba Centellae extract comprises the steps: to pulverize Herba Centellae to be placed on equipped with in 0.4% sodium bicarbonate solution, Herba Centellae mass ratio in the solution is at 10-30%, it is 8 with breast acid for adjusting pH value, it is warming up to 45 DEG C, add the mixed enzyme of Herba Centellae quality 1.5%, stir, enzymolysis 40min, subsequently in electric field intensity 30kV/cm, burst length 500 μ s, carry out high voltage electric field under the conditions of pulse frequency 200-300Hz to process 8 minutes, then room temperature 400W, 40KHz condition supersound process 12min；The enzymolysis solution of above-mentioned process is drying to obtain Herba Centellae extract through 200 eye mesh screen filter freezings；The quality group of described mixed enzyme becomes: saccharifying enzyme: pectase: cellulase=1:3:7.
The preparation method of described Jojoba seed extract comprises the following steps: Jojoba seed adds the water of its quality 5 times after pulverizing, and at electric field intensity 30kV/cm, burst length 450 μ s, carries out high voltage electric field and extract 8 minutes under the conditions of pulse frequency 200Hz；It is 8 with sodium carbonate regulation pH value, adds the compound enzyme of Jojoba seed quality 1.5%, in 52 DEG C of enzymolysis 60min；Enzymolysis solution lyophilization, low-temperature grinding to particle diameter are that 0.2mm i.e. obtains Jojoba seed extract；Described compound enzyme is that cellulase, xylanase, laccase, pectase 6:8:2:1 in mass ratio uniformly mixes.
The preparation method of described oat extract, comprise the steps: by after Herba bromi japonici surface sprinkling 3% lactic acid solution in-21-25 DEG C of freezing 30min；Pulverizing immediately, particle diameter is 0.5mm；Adding the deionized water of ground product quality 4 times, mix homogeneously, is 6 with Fructus Citri Limoniae acid for adjusting pH value, add the mixed enzyme of Herba bromi japonici quality 4%, stir, adjust temperature and be 65-70 DEG C, enzymolysis 70min, enzymolysis solution filters through 200 eye mesh screens, and freeze-dried i.e. the obtaining of filtrate i.e. obtains oat extract.The quality group of described mixed enzyme becomes: saccharifying enzyme: pectase: cellulase=1:1:8.
Described Camellia method for preparing extractive is: Camellia is crushed to particle diameter 0.2mm, add the water of Camellia quality 2 times, it is 5 with breast acid for adjusting pH value, it is warming up to 45 DEG C, add the mixed enzyme of Camellia quality 4%, stir, enzymolysis 60min, enzymolysis solution filters through 200 eye mesh screens, and filtrate is freeze-dried i.e. obtains Camellia extract；The quality group of described mixed enzyme becomes: pectase: cellulase=1:8.
Aloe vera extract preparation method is as follows: take Aloe vulgaris in-18-23 DEG C of freezing 5-10min；It is crushed to particle diameter 0.3-1mm immediately；Put in container and add 2-3% sodium carbonate liquor regulation pH value be 7-9, be warming up to 60-70 DEG C, insulation, be subsequently adding the mixed enzyme enzymolysis 30-50min of mixed material gross weight 2-5%, filter, obtain filtrate；Filtrate is through ultrafiltration lyophilization and get final product.Described mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:8-15:2 in mass ratio.
Semen Vitis viniferae extract preparation method is as follows: in-23 DEG C of freezing 50min after Semen Vitis viniferae surface sprinkling lactic acid；It is crushed to particle diameter 0.5mm immediately；Put in container and add 2% sodium carbonate liquor regulation pH value be 8, be warming up to 65 DEG C, insulation, be subsequently adding the mixed enzyme enzymolysis 40min of mixed material gross weight 3%, filter, obtain filtrate；Filtrate is through ultrafiltration postlyophilization and get final product.Described mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:10:2 in mass ratio.
Ferment powder preparation method is as follows:
The big grain materials such as the fermentation liquid using pickle fermentation to produce discharge in latter stage is raw material, the dish leaf being filtered to remove in fermentation liquid obtain fermented liquid, and fermented liquid uses the drying meanss such as lyophilization to prepare ferment powder.
Cryodesiccated condition is :-30 DEG C of pre-freezes 6 hours；Then lyophilizing is until water content is less than 5%.Pickle production method is prepared with reference to Chinese patent 201110421967.3 or 201210310308.7.
The preparation method of Lactobacillus plantarum mycopowder is as follows:
Lactobacillus plantarum powdery mycopowder, production stage is as follows: slant strains is transferred to fluid medium the volume required that spreads cultivation step by step；The bacterium solution obtained spreading cultivation is centrifuged separating, and collects precipitation thalline；In precipitation thalline, add protective agent and be diluted；Utilizing drying equipment to prepare powdery microbial inoculum, it is (8～9.0) * (10 that said method prepares viable count in Lactobacillus plantarum mycopowder10) individual/gram, starch can be added during mycopowder mixing and dextrin realizes the proper ratio of each bacterium viable count.Described Lactobacillus plantarum is CGMCC 11763.
Prepare 100 kilograms of mask product raw materials, production method:
A phase raw material composition and mass ratio (mass percent %):
Sodium acrylate/sodium acryloyldimethyl taurate copolymers 0.6,2-Methylpentadecane 2, polydimethylsiloxane 0.7, caprylic/capric triglyceride 1.8, polysorbate-20 0.6.
B phase raw material forms: glycerol 2, methyl hydroxybenzoate 0.1, carbomer 0.08, allantoin 0.1, glycine betaine 2, hyaluronic acid 0.03, glycyrrhizic acid dipotassium 0.2, EDETATE SODIUM 0.03
C phase raw material forms: triethanolamine 0.08.
D phase raw material forms: polydimethylsiloxane 0.5, Methylisothiazolinone 0.1, essence 0.06.
Remaining is water.
Production method is as follows:
Adding water to be slowly added under aqueous phase pot, high-speed stirred raw material carbomer, heated and stirred is to being completely dissolved；
It is sequentially added into the B whole component of phase raw material, is heated with stirring to be completely dissolved, and be warming up to 85 DEG C of insulations 30 minutes；
Aqueous phase suction emulsifying pot will be cooled；
Adding when 70 DEG C and be pre-mixed uniform A phase component, high speed homogenization stopped homogenizing after 5 minutes,
Insulated and stirred cooled down after 10 minutes；
Add C phase and D phase raw material when 45 DEG C, stir to mix homogeneously the plant extract combination agent adding 20%.
38 DEG C of dischargings or coat non-woven fabrics and be prepared as mask product.
Described Lactobacillus plantarum is CGMCC 11763.
Embodiment 2 is basic with example 1
The present invention provides a kind of moisturizing, rehabilitation plant extractive composition and the application in cosmetics thereof:
Moisturizing, the parts by weight of rehabilitation plant extractive composition form as follows:
Camellia extract 15, aloe vera extract 20, oat extract 8, Jojoba seed extract 15, Herba Centellae extract 10, Semen Vitis viniferae extract 2, ferment powder 8, Lactobacillus plantarum mycopowder 2.
The preparation method of described Herba Centellae extract comprises the steps: to pulverize Herba Centellae to be placed on equipped with in 0.3% sodium bicarbonate solution, it is 7 with breast acid for adjusting pH value, it is warming up to 40-50 DEG C, add the mixed enzyme of Herba Centellae quality 1-2%, stir, enzymolysis 35min, in electric field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high voltage electric field and processes 10 minutes under the conditions of pulse frequency 200-300Hz, then room temperature 400W, 40KHz condition supersound process 10min；The enzymolysis solution of above-mentioned process is drying to obtain Herba Centellae extract through 100-300 eye mesh screen filter freezing；The quality group of described mixed enzyme becomes: saccharifying enzyme: pectase: cellulase=1:5:10.
The preparation method of described Jojoba seed extract comprises the following steps: Jojoba seed adds the water of its quality 7 times after pulverizing, and at electric field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high voltage electric field and extract 10 minutes under the conditions of pulse frequency 200-300Hz；It is 7 with sodium carbonate regulation pH value, adds the compound enzyme of Jojoba seed quality 1%, in 48 DEG C of enzymolysis 50min；Enzymolysis solution lyophilization, low-temperature grinding to particle diameter are that 0.1mm i.e. obtains Jojoba seed extract；Described compound enzyme is that cellulase, xylanase, laccase, pectase 6:10:2:1 in mass ratio uniformly mixes.
The preparation method of described oat extract, comprise the steps: by after Herba bromi japonici surface sprinkling 2% lactic acid solution in-21-25 DEG C of freezing 10min；Pulverizing immediately, particle diameter is 1mm；Adding the deionized water of ground product quality 3 times, mix homogeneously, is 5 with Fructus Citri Limoniae acid for adjusting pH value, add the mixed enzyme of Herba bromi japonici quality 3%, stir, adjust temperature and be 65-70 DEG C, enzymolysis 80min, enzymolysis solution filters through 100-300 eye mesh screen, and freeze-dried i.e. the obtaining of filtrate i.e. obtains oat extract.The quality group of described mixed enzyme becomes: saccharifying enzyme: pectase: cellulase=1:2:6.
Described Camellia method for preparing extractive is: Camellia is crushed to particle diameter 0.1-0.3mm, add the water of Camellia quality 3 times, it is 5 with breast acid for adjusting pH value, it is warming up to 40 DEG C, add the mixed enzyme of Camellia quality 3%, stir, enzymolysis 70min, enzymolysis solution filters through 100-300 eye mesh screen, and filtrate is freeze-dried i.e. obtains Camellia extract；The quality group of described mixed enzyme becomes: pectase: cellulase=1:6.
Aloe vera extract preparation method is as follows: take Aloe vulgaris in-18-23 DEG C of freezing 10min；It is crushed to particle diameter 1mm immediately；Put in container and add 2% sodium carbonate liquor regulation pH value be 9, be warming up to 60-70 DEG C, insulation, be subsequently adding the mixed enzyme enzymolysis 50min of mixed material gross weight 2%, filter, obtain filtrate；Filtrate is through ultrafiltration lyophilization and get final product.Described mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:10:2 in mass ratio.
Semen Vitis viniferae extract preparation method is as follows: take after Semen Vitis viniferae surface sprinkling lactic acid in-18-23 DEG C of freezing 50min；It is crushed to particle diameter 0.3mm immediately；Put in container and add 2% sodium carbonate liquor regulation pH value be 7, be warming up to 70 DEG C, insulation, be subsequently adding the mixed enzyme enzymolysis 50min of mixed material gross weight 2%, filter, obtain filtrate；Filtrate is through ultrafiltration postlyophilization and get final product.Described mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:15:2 in mass ratio.
Ferment powder preparation method is as follows:
The big grain materials such as the fermentation liquid using pickle fermentation to produce discharge in latter stage is raw material, the dish leaf being filtered to remove in fermentation liquid obtain fermented liquid, and fermented liquid uses the drying meanss such as lyophilization to prepare ferment powder.
Cryodesiccated condition is :-30 degree pre-freezes 6 hours；Then lyophilizing is until water content is less than 5%.Pickle production method is prepared with reference to Chinese patent 201110421967.3 or 201210310308.7.
Described Lactobacillus plantarum is CGMCC 11763.
One of facial film production method:
A phase raw material composition and mass ratio (mass percent):
Sodium acrylate/sodium acryloyldimethyl taurate copolymers 0.5-0.8,2-Methylpentadecane 2, polydimethylsiloxane 0.5-0.8, caprylic/capric triglyceride 1.5-2, polysorbate-20 0.5-0.8.
B phase raw material forms: glycerol 2, methyl hydroxybenzoate 0.1-0.2, carbomer 0.07-0.1, allantoin 0.1-0.2, glycine betaine 2, hyaluronic acid 0.01-0.05, glycyrrhizic acid dipotassium 0.1-0.3, EDETATE SODIUM 0.01-0.05
C phase raw material forms: triethanolamine 0.07-0.1,
D phase raw material forms: polydimethylsiloxane 0.4-0.6, Methylisothiazolinone 0.1-0.2, essence 0.05-0.08.
Remaining is water.
Production method is as follows:
Adding water to be slowly added under aqueous phase pot, high-speed stirred raw material carbomer, heated and stirred is to being completely dissolved；
It is sequentially added into the B whole component of phase raw material, is heated with stirring to be completely dissolved, and be warming up to 85 DEG C of insulations 30 minutes；
Aqueous phase suction emulsifying pot will be cooled；
Adding when 70 DEG C and be pre-mixed uniform A phase component, high speed homogenization stops homogenizing after 5 minutes, homogenization condition is；
Insulated and stirred cooled down after 10 minutes；
Add C phase and D phase raw material when 45 DEG C, stir to mix homogeneously the plant extract combination agent adding 30%.
38 DEG C of dischargings or coat non-woven fabrics and be prepared as mask product.
Embodiment 3
The present invention provides a kind of moisturizing, rehabilitation plant extractive composition and the application in cosmetics thereof:
Moisturizing, the parts by weight of rehabilitation plant extractive composition form as follows:
Camellia extract 30, aloe vera extract 10, oat extract 8, Jojoba seed extract 15, Herba Centellae extract 5, Semen Vitis viniferae extract 2, ferment powder 5, Lactobacillus plantarum mycopowder 2.
Embodiment 4
One of facial film composition and preparation production method:
A phase raw material composition and mass ratio (mass percent):
Sodium acrylate/sodium acryloyldimethyl taurate copolymers 0.7,2-Methylpentadecane 2, polydimethylsiloxane 0.7, caprylic/capric triglyceride 1.7, polysorbate-20 0.6.
B phase raw material forms: glycerol 2, methyl hydroxybenzoate 0.2, carbomer 0.08, allantoin 0.1, glycine betaine 2, hyaluronic acid 0.03, glycyrrhizic acid dipotassium 0.2, EDETATE SODIUM 0.03
C phase raw material forms: triethanolamine 0.09,
D phase raw material forms: polydimethylsiloxane 0.5, Methylisothiazolinone 0.1, essence 0.06.
Remaining is water.
Production method is as follows:
Adding water to be slowly added under aqueous phase pot, high-speed stirred raw material carbomer, heated and stirred is to being completely dissolved；
It is sequentially added into the B whole component of phase raw material, is heated with stirring to be completely dissolved, and be warming up to 85 DEG C of insulations 30 minutes；
Aqueous phase suction emulsifying pot will be cooled；
Adding when 70 DEG C and be pre-mixed uniform A phase component, high speed homogenization stops homogenizing after 5 minutes, homogenization condition is:；
Insulated and stirred cooled down after 10 minutes；
Add C phase and D phase raw material when 45 DEG C, stir to mix homogeneously the plant extract combination agent adding 20%.
38 DEG C of dischargings or coat non-woven fabrics and be prepared as mask product.
Using effect:
Facial film effect test: GB15979-2002 standard, TWO skin detection is non-stimulated material.
Moisture-keeping efficacy: with reference to QB/T 4,256 2011 " cosmetics moisture-keeping efficacy evaluation guide ".
This comparative test, with reference to " cosmetics moisture-keeping efficacy evaluation guide " (QB/T4256-2011), after being intended for single use mask by volunteer, utilizes instrument Non-Invasive evaluation mask at 6 hours interior moistening effects in constant-temperature constant-humidity environment.In test process, test surfaces pad pasting and blank are randomly distributed in the left and right inner forearm of experimenter.Product is intended for single use, and application time is 20 minutes.After using 0.5 hour, 1 hour, 2 hours, 4 hours and 6 hours with product before product uses respectively, moisture of skin tester (Corneometer) is utilized to use district and blank district to carry out keratodermatitis moisture test respectively product.Film sample has 50 Radix Ginseng adductions to complete test.Product is being tested by half an hour after, and skin moisture content averagely adds 48.56 units, and the highest reaches 55 units.
Use rowIn tableThe mask product of the compositions application preparation that combination obtains, chooses 196 people and carries out effect test, and product effect is shown inTable 1,
In mask product, vegetable composition composition addsTable 1:
Remarks: Camellia extract (A), aloe vera extract (B), Jojoba seed extract (C), Herba Centellae extract (D), Semen Vitis viniferae extract (E), oat extract (F), ferment powder (G), Lactobacillus plantarum (H) whitening (X), skin elasticity (W), skin complexion improves (Z)
Product adds the interpolation of vegetable composition composition and measures the interpolation of ratio intermediate value；RowIn table38,39 for using example 1, the mask product of vegetable composition in example 2, and other are the mask product effect adding different vegetable composition according to form.
Claims (9)
1. a moisturizing, rehabilitation plant extractive composition, described moisturizing, the parts by weight of rehabilitation plant extractive composition form as follows: Camellia extract 15-30, aloe vera extract 10-20, oat extract 8-15, Jojoba seed extract 10-15, Herba Centellae extract 5-10, Semen Vitis viniferae extract 2-5, ferment powder 5-8, Lactobacillus plantarum mycopowder 2-5.
A kind of moisturizing the most according to claim 1, rehabilitation plant extractive composition, the preparation method of described Herba Centellae extract comprises the steps: to pulverize Herba Centellae to be placed on equipped with in 0.3-0.5% sodium bicarbonate solution, it is 7-8 with breast acid for adjusting pH value, it is warming up to 40-50 DEG C, add the mixed enzyme of Herba Centellae quality 1-2%, stir, enzymolysis 35-50min, subsequently in electric field intensity 20-40kV/cm, burst length 400-500 μ s, carry out high voltage electric field under the conditions of pulse frequency 200-300Hz to process 5-10 minute, then room temperature 400W, 40KHz condition supersound process 10-15min；The enzymolysis solution of above-mentioned process is drying to obtain Herba Centellae extract through 100-300 eye mesh screen filter freezing；The quality group of described mixed enzyme becomes: saccharifying enzyme: pectase: cellulase=1:2-5:5-10；Described Lactobacillus plantarum is CGMCC 11763.
A kind of moisturizing, rehabilitation plant extractive composition the most according to claim 1, the preparation method of described Jojoba seed extract comprises the following steps: Jojoba seed adds the water of its quality 3-7 times after pulverizing, in electric field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high voltage electric field and extracts 6-10 minute under the conditions of pulse frequency 200-300Hz；It is 7-9 with sodium carbonate regulation pH value, adds the compound enzyme of Jojoba seed quality 1-2%, in 48-55 DEG C of enzymolysis 50-80min；Enzymolysis solution lyophilization, low-temperature grinding to particle diameter are that 0.1-0.3mm i.e. obtains Jojoba seed extract；Described compound enzyme is that cellulase, xylanase, laccase, pectase 6:5-10:2:1 in mass ratio uniformly mixes.
A kind of moisturizing, rehabilitation plant extractive composition the most according to claim 1, the preparation method of described oat extract, comprise the steps: by after Herba bromi japonici surface sprinkling 2-5% lactic acid solution in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, particle diameter is 0.1-1mm；Add the deionized water of ground product quality 3-5 times, mix homogeneously, it is 5-7 with Fructus Citri Limoniae acid for adjusting pH value, add the mixed enzyme of Herba bromi japonici quality 3-5%, stir, adjust temperature and be 65-70 DEG C, enzymolysis 50-80min, enzymolysis solution filters through 100-300 eye mesh screen, and freeze-dried i.e. the obtaining of filtrate i.e. obtains oat extract；The quality group of described mixed enzyme becomes: saccharifying enzyme: pectase: cellulase=1:1-2:6-10.
A kind of moisturizing, rehabilitation plant extractive composition the most according to claim 1, described Camellia method for preparing extractive is: Camellia is crushed to particle diameter 0.1-0.3mm, add the water of Camellia quality 1-3 times, it is 5-6 with breast acid for adjusting pH value, is warming up to 40-50 DEG C, add the mixed enzyme of Camellia quality 3-5%, stir, enzymolysis 55-80min, enzymolysis solution filters through 100-300 eye mesh screen, and filtrate is freeze-dried i.e. obtains Camellia extract；The quality group of described mixed enzyme becomes: pectase: cellulase=1:6-10.
A kind of moisturizing, rehabilitation plant extractive composition the most according to claim 1, aloe vera extract preparation method is as follows: take Aloe vulgaris in-18-23 DEG C of freezing 5-10min；It is crushed to particle diameter 0.3-1mm immediately；Put in container and add 2-3% sodium carbonate liquor regulation pH value be 7-9, be warming up to 60-70 DEG C, insulation, be subsequently adding the mixed enzyme enzymolysis 30-50min of mixed material gross weight 2-5%, filter, obtain filtrate；Filtrate is through ultrafiltration lyophilization and get final product；Described mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:8-15:2 in mass ratio.
7., according to the arbitrary described a kind of moisturizing of claim 1-6, rehabilitation plant extractive composition, Semen Vitis viniferae extract preparation method is as follows: in-18-23 DEG C of freezing 30-50min after Semen Vitis viniferae surface sprinkling lactic acid；It is crushed to particle diameter 0.3-1mm immediately；Put in container and add 2-3% sodium carbonate liquor regulation pH value be 7-9, be warming up to 60-70 DEG C, insulation, be subsequently adding the mixed enzyme enzymolysis 30-50min of mixed material gross weight 2-5%, filter, obtain filtrate；Filtrate is through ultrafiltration postlyophilization and get final product；Described mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:8-15:2 in mass ratio；Ferment powder preparation method is as follows: the big grain materials such as the fermentation liquid using pickle fermentation to produce discharge in latter stage is raw material, the dish leaf being filtered to remove in fermentation liquid obtain fermented liquid, and fermented liquid uses the drying meanss such as lyophilization to prepare ferment powder.
8. according to the arbitrary described a kind of moisturizing of claim 1-7, rehabilitation plant extractive composition, moisturizing, the parts by weight of rehabilitation plant extractive composition form as follows: Camellia extract 20, aloe vera extract 15, oat extract 10, Jojoba seed extract 12, Herba Centellae extract 8, Semen Vitis viniferae extract 3, ferment powder 6, Lactobacillus plantarum mycopowder 3；The preparation method of described Herba Centellae extract comprises the steps: to pulverize Herba Centellae to be placed on equipped with in 0.4% sodium bicarbonate solution, it is 8 with breast acid for adjusting pH value, it is warming up to 45 DEG C, add the mixed enzyme of Herba Centellae quality 1.5%, stir, enzymolysis 40min, subsequently in electric field intensity 30kV/cm, burst length 500 μ s, carries out high voltage electric field and processes 8 minutes under the conditions of pulse frequency 200-300Hz, then room temperature 400W, 40KHz condition supersound process 12min；The enzymolysis solution of above-mentioned process is drying to obtain Herba Centellae extract through 200 eye mesh screen filter freezings；The quality group of described mixed enzyme becomes: saccharifying enzyme: pectase: cellulase=1:3:7；
The preparation method of described Jojoba seed extract comprises the following steps: Jojoba seed adds the water of its quality 5 times after pulverizing, and at electric field intensity 30kV/cm, burst length 450 μ s, carries out high voltage electric field and extract 8 minutes under the conditions of pulse frequency 200Hz；It is 8 with sodium carbonate regulation pH value, adds the compound enzyme of Jojoba seed quality 1.5%, in 52 DEG C of enzymolysis 60min；Enzymolysis solution lyophilization, low-temperature grinding to particle diameter are that 0.2mm i.e. obtains Jojoba seed extract；Described compound enzyme is that cellulase, xylanase, laccase, pectase 6:8:2:1 in mass ratio uniformly mixes；
The preparation method of described oat extract, comprise the steps: by after Herba bromi japonici surface sprinkling 3% lactic acid solution in-21-25 DEG C of freezing 30min；Pulverizing immediately, particle diameter is 0.5mm；Adding the deionized water of ground product quality 4 times, mix homogeneously, is 6 with Fructus Citri Limoniae acid for adjusting pH value, add the mixed enzyme of Herba bromi japonici quality 4%, stir, adjust temperature and be 65-70 DEG C, enzymolysis 70min, enzymolysis solution filters through 200 eye mesh screens, and freeze-dried i.e. the obtaining of filtrate i.e. obtains oat extract；The quality group of described mixed enzyme becomes: saccharifying enzyme: pectase: cellulase=1:1:8；
Described Camellia method for preparing extractive is: Camellia is crushed to particle diameter 0.2mm, add the water of Camellia quality 2 times, it is 5 with breast acid for adjusting pH value, it is warming up to 45 DEG C, add the mixed enzyme of Camellia quality 4%, stir, enzymolysis 60min, enzymolysis solution filters through 200 eye mesh screens, and filtrate is freeze-dried i.e. obtains Camellia extract；The quality group of described mixed enzyme becomes: pectase: cellulase=1:8；
Aloe vera extract preparation method is as follows: take Aloe vulgaris in-18-23 DEG C of freezing 5-10min；It is crushed to particle diameter 0.3-1mm immediately；Put in container and add 2-3% sodium carbonate liquor regulation pH value be 7-9, be warming up to 60-70 DEG C, insulation, be subsequently adding the mixed enzyme enzymolysis 30-50min of mixed material gross weight 2-5%, filter, obtain filtrate；Filtrate is through ultrafiltration lyophilization and get final product.Described mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:8-15:2 in mass ratio；Semen Vitis viniferae extract preparation method is as follows: in-23 DEG C of freezing 50min after Semen Vitis viniferae surface sprinkling lactic acid；It is crushed to particle diameter 0.5mm immediately；Put in container and add 2% sodium carbonate liquor regulation pH value be 8, be warming up to 65 DEG C, insulation, be subsequently adding the mixed enzyme enzymolysis 40min of mixed material gross weight 3%, filter, obtain filtrate；Filtrate is through ultrafiltration postlyophilization and get final product；
Described mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:10:2 in mass ratio；
Ferment powder preparation method is as follows: the big grain materials such as the fermentation liquid using pickle fermentation to produce discharge in latter stage is raw material, the dish leaf being filtered to remove in fermentation liquid obtain fermented liquid, and fermented liquid uses the drying meanss such as lyophilization to prepare ferment powder；Described Lactobacillus plantarum is CGMCC 11763.
9. utilize the mask product prepared by the arbitrary described a kind of moisturizing of claim 1-8, rehabilitation plant extractive composition, comprise the following steps: adding water to be slowly added under aqueous phase pot, high-speed stirred raw material carbomer, heated and stirred is to being completely dissolved；It is sequentially added into the B whole component of phase raw material, is heated with stirring to be completely dissolved, and be warming up to 85 DEG C of insulations 30 minutes；Aqueous phase suction emulsifying pot will be cooled；Adding when 70 DEG C and be pre-mixed uniform A phase component, high speed homogenization stops homogenizing after 5 minutes, insulated and stirred cooled down after 10 minutes；Add C phase and D phase raw material when 45 DEG C, stir to mix homogeneously and add the plant extract combination agent that mass ratio is 15-30%；38 DEG C of dischargings or coat non-woven fabrics and be prepared as mask product；
A phase raw material composition and mass ratio: sodium acrylate/sodium acryloyldimethyl taurate copolymers 0.5-0.8,2-Methylpentadecane 2, polydimethylsiloxane 0.5-0.8, caprylic/capric triglyceride 1.5-2, polysorbate-20 0.5-0.8；
B phase raw material composition and mass ratio: glycerol 2, methyl hydroxybenzoate 0.1-0.2, carbomer 0.07-0.1, allantoin 0.1-0.2, glycine betaine 2, hyaluronic acid 0.01-0.05, glycyrrhizic acid dipotassium 0.1-0.3, EDETATE SODIUM 0.01-0.05；
C phase raw material composition and mass ratio: triethanolamine 0.07-0.1；
D phase raw material composition and mass ratio: polydimethylsiloxane 0.4-0.6, Methylisothiazolinone 0.1-0.2, essence 0.05-0.08；Remaining is water.
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| CN108096136A (en) * | 2017-12-12 | 2018-06-01 | 常州莱尚纺织品有限公司 | A kind of preparation method of non-stimulated spot-removing ointment |
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| CN108324659A (en) * | 2018-05-17 | 2018-07-27 | 北京养心堂健康科技有限公司 | A kind of moisturizing anti-oxidant face mask powder |
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| CN108324659A (en) * | 2018-05-17 | 2018-07-27 | 北京养心堂健康科技有限公司 | A kind of moisturizing anti-oxidant face mask powder |
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| CN110755316A (en) * | 2019-10-10 | 2020-02-07 | 澳宝化妆品（惠州）有限公司 | Grape seed extract and skin care composition containing same |
| CN110721135A (en) * | 2019-11-29 | 2020-01-24 | 广州欧正化妆品技术研究院有限公司 | Fullerene-containing solid concentrated dry mask with moisturizing, repairing and anti-aging effects and preparation method thereof |
| CN112494403A (en) * | 2020-04-10 | 2021-03-16 | 广东柏俐臣生物科技有限公司 | Oat fermentation liquor and preparation method and application thereof |
| KR102280751B1 (en) * | 2020-12-21 | 2021-07-22 | (주)지에프씨생명과학 | Lactobacillus plantarum YoungBiome2 and Titrated Extract of Fermented Centella Asiatica Manufactured Using Thereof |
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| CN113893207A (en) * | 2021-11-09 | 2022-01-07 | 食妆技术研究(山东)有限公司 | Edible fruit and vegetable enzyme skin care product and preparation method thereof |
| CN114392048A (en) * | 2021-12-21 | 2022-04-26 | 青岛湃睿生物科技有限公司 | Preparation process of cold compress gel |
| CN114767608A (en) * | 2022-05-10 | 2022-07-22 | 广东瀚润生物科技有限公司 | Natural plant maintenance and rejuvenation compound skin care product raw material, and preparation method and application thereof |
| CN115137673A (en) * | 2022-08-11 | 2022-10-04 | 北京茂思商贸有限公司 | Fermented cosmetic composition and preparation method and application thereof |
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