A kind of moisturizing, rehabilitation plant extractive composition and its application in cosmetics
Technical field: the invention belongs to cosmetic fields, in particular to moisture saver mask product.
Background technique
The fundamental of healthy skin is smooth, full, high resilience.With the increase at age, in keratoderma
Moisturizing factor content gradually decreases, and the hydratability of skin is caused to decline, and shows as dry skin, corrugation, relaxation, aging is presented
Appearance, current atmosphere protection layer is destroyed in addition, and ultraviolet radiation is serious, dust, haze, sandstorm, PM2.5, computer
The environmental pollutions such as radiation, secondhand smoke, vehicle exhaust, industrial pollution, exhaust gas can all lead to dry skin, stimulation, allergy, skin
Water shortage, sensitivity, fat secretion are unbalance, aging, the problem that also blackhead, pore can be made coarse.In addition to this, urban life pressure
Greatly, at the same fatigue, stay up late, with advancing age, cause dry skin relax, tarnish, skin resistance decline, most hold
Vulnerable to external invasion, cause allergy, dimness, relaxation dry skin.
Facial mask is a kind of people seeking beauty's commonly cosmetics, and facial mask is many kinds of, and effect is also different.It is former according to production
The difference of material can be divided into moisturizing, whitening, nti-freckle and other functional masks.Such as releive skin, enhancing skin metabolic detoxification effect,
Skin toxin is excluded, improves skin quality, become brighter glossy using the rear colour of skin, improves the wax yellow, obscure, dry of the colour of skin and is stranded
It disturbs.It can also enhance or increase other effects, such as elastic force moisturizing, compact lifting, skin-moisturizing, skin, whitening despeckle, wrinkle resistant anti-ageing
Always, anti-inflammatory toxin expelling, prevention and treatment dark sore etc..Initial stage facial mask mostly uses greatly synthetic as base stock, with effect in formula
The improvement of additive, consumer have further pursuit to purer extraction, more natural, safer, more effective mask product.
The Chinese patent of 104352421 A of Publication No. CN discloses " a kind of skin rejuvenating facial mask liquid ", the tender skin face of the invention
Film liquid, ingredient include 1,3 butanediols 2.5%~3.5%, xanthan gum 0.15%~0.25%, ethylenediamine by mass percentage
Tetraacethyl disodium 0.04%~0.06%, glycerol 4%~6%, 0.05%~1.2%, 1,2 pentanediol 1.5% of dipotassium glycyrrhizinate
~3.5%, Sodium Hyaluronate 0.03%~0.2%, aloe barbadensis Miller leaf juice 3%~35%, tremella extract 1%~15%,
Purslane extract 0.5%~6%, β glucose 0.4%~7%, chamomile extracting solution 0.5%~6%, honey 0.5%~
6%, Chlorphenesin 0.1%~0.25% etc..Compared with prior art, the present invention there is anti-aging, and it is crease-resistant, the advantages that tender skin,
The advantages that sun-damaged skin, sensitivity of releiving can also be repaired simultaneously.
The patent of invention of 105106064 A of Publication No. CN discloses " a kind of moisturizing Wash-free mask and its preparation side
Method " is by solid grease, liquid fat, emulsifier, rheology control agent, moisturizer, plant extracts and deionized water group
At;In addition, the present invention also provides the preparation methods of the facial mask.The made facial mask of the present invention compared with prior art, made production
Product especially moisten mildly, and highly-safe, the compatibility and compatibility between grease are preferable, utilize the high moistening effect of oat beta-glucan
Collocation polyalcohols moisturizer, moistening effect is splendid, and natural bisabolol protects sensitive skin, adds Gotu Kola P.E, promotees
It is flexible with rear skin into the effect of wound healing and antibacterial anti-inflammatory, gloss is moistened, is felt to skin silk smooth felling, it is maximum excellent
Point is no membrane material, no excessive packaging, reduces the wasting of resources and is conducive to sustainable development.
104814918 A disclosure of the invention of Publication No. CN " a kind of replenishing water and preserving moisture facial mask liquid and its manufacturing method ", by weight
Percentages are measured, are made of the following components: polymer hyaluronic acid sodium: 0.1-2.0%, oligomerization Sodium Hyaluronate: 0.1-
2%, disodium ethylene diamine tetraacetate (EDTA-2Na): 0.01-0.2%, anti-sensitizer: 0.01-5.0%, the first moisturizer: 1-8%,
Preservative: 0.01-0.5%, the second moisturizer: 1-6% Bacillus acidi lactici/pear juice tunning filtrate: 0.5-2% deionized water:
Its surplus.Facial mask liquid of the invention, preparation method is simple, utilizes " dual moisturizing factor "-polymer hyaluronic acid sodium and widow
Poly- Sodium Hyaluronate, and " biotic factor "-Bacillus acidi lactici.
Chinese patent: the preparation method of bioanalysis Rapid Fermentation pickles, Patent No. 201110421967.3;Disclose life
The preparation method of object method Rapid Fermentation pickles, the invention belongs to field of vegetable deep-processing, in particular to are produced using composite bacterium powder
The method of pickles.The present invention solves the technical issues of quickly production high-quality fermentation pickled vegetable.Key step of the present invention has: (1)
Charging sealing: be put into container after vegetable raw-material cutting, be added containing acetobacter, S. cervisiae composite bacterium powder and contain
The auxiliary material of salt, sealing container;(2) ferment: control temperature then controls temperature in 15- in 23-36 DEG C of progress prior fermentation
25 DEG C of progress post-fermentations, entire fermentation time was at 20-40 hours;(3) dehydration allotment: fermentation, which finishes, sloughs pickles moisture, is added
Flavoring for mixture is uniform.The present invention is suitable for the pickle production of different scales, can be obviously shortened the production time, and pickles produce
Product are full of nutrition, pure taste, and this product has broad application prospects in pickle production field.
Chinese patent " preparation method of the prebiotic pickles of bioanalysis Rapid Fermentation, application number: 201210310308.7 applications
Day: the invention discloses the preparation methods of the prebiotic pickles of bioanalysis Rapid Fermentation, and the invention belongs to field of vegetable deep-processing, especially
Relate to the use of the method for composite bacterium powder production pickles.The present invention solves the technical issues of quickly production high-quality fermentation pickled vegetable.
Key step of the present invention has: (1) charging sealing: being put into container after vegetable raw-material cutting, is added containing acetobacter, wine brewing ferment
The composite bacterium powder of female bacterium and auxiliary material containing salt, sealing container;(2) ferment: control temperature is in 23-36 DEG C of progress hair early period
Ferment then controls temperature in 15-25 DEG C of progress post-fermentation, and entire fermentation time was at 20-40 hours;(3) dehydration allotment: fermentation
It finishes and sloughs pickles moisture, it is uniform that flavoring for mixture is added.The present invention is suitable for the pickle production of different scales, Ke Yiming
Aobvious to shorten the production time, kimchi products are full of nutrition, pure taste, before this product has wide application in pickle production field
Scape.
The patent of invention of Publication No. CN 103976913A discloses " a kind of face mask of traditional Chinese medicine for moisturizing ", including with
The medicine preparation of lower parts by weight forms: the rhizome of chuanxiong of 20-30 parts by weight, the aloe of 20-30 parts by weight, 15-25 parts by weight
Several Siraitia grosvenoriis, the asparagus fern of 15-25 parts by weight, the seaweed of 35-45 parts by weight, the honeysuckle of 15-25 parts by weight, 5-15
The Radix Glycyrrhizae of parts by weight.The invention have promoting blood circulation moisturize, clearing heat and moistening lung, moisturizing moisturizing, dispel dry moisturizing the effect of.
Although current miscellaneous facial mask is innumerable, the replenishing water and preserving moisture effect of moisture saver mask is also not fully up to expectations,
Safety is unstable, could not fundamentally improve skin problem etc., therefore, it is necessary to research and develop a kind of natural safety, moisturizing, water lock
The good moisture saver mask liquid of effect.
Summary of the invention:
The present invention provides a kind of moisturizing, rehabilitation plant extractive composition and its application in cosmetics: moisturizing, reparation
The parts by weight composition of composition of plant extracts is as follows:
Camellia seed extract 15-30, aloe vera extract 10-20, oat extract 8-15, Jojoba seed extract
10-15, Gotu Kola P.E 5-10, grape seed extract 2-5, ferment powder 5-8, lactobacillus plantarum bacterium powder 2-5.
The preparation method of the Gotu Kola P.E, which includes the following steps: to crush centella, to be placed on equipped with 0.3-
It is 7-8 with newborn acid for adjusting pH value in 0.5% sodium bicarbonate solution, is warming up to 40-50 DEG C, the mixed of centella quality 1-2% is added
Synthase stirs evenly, and 35-50min is digested, then in electric field strength 20-40kV/cm, burst length 400-500 μ s, pulse frequency
It carries out high voltage electric field under the conditions of rate 200-300Hz to handle 5-10 minutes, then room temperature 400W, 40KHz condition is ultrasonically treated 10-
15min;The enzymolysis liquid of above-mentioned processing is drying to obtain Gotu Kola P.E through 100-300 mesh screen filter freezing;The mixed enzyme
Quality group become: carbohydrase: pectase: cellulase=1:2-5:5-10.
The preparation method of the Jojoba seed extract is the following steps are included: its quality 3-7 is added after crushing in Jojoba seed
Water again, carries out high pressure under the conditions of electric field strength 20-40kV/cm, burst length 400-500 μ s, pulse frequency 200-300Hz
Electric field extracts 6-10 minutes;Adjusting pH value with sodium carbonate is 7-9, the compound enzyme of Jojoba seed quality 1-2% is added, in 48-55
DEG C enzymatic hydrolysis 50-80min;Enzymolysis liquid freeze-drying, low-temperature grinding to partial size are 0.1-0.3mm up to Jojoba seed extract;Institute
Compound enzyme is stated uniformly to mix for cellulase, zytase, laccase, pectase 6:5-10:2:1 in mass ratio.
The preparation method of the oat extract includes the following steps: after 2-5% lactic acid solution is sprayed on oat surface
In-21-- 25 DEG C of freezing 10-30min;It is crushed immediately, partial size 0.1-1mm;Be added 3-5 times of crushed material quality go from
Sub- water is uniformly mixed, is 5-7 with lemon acid for adjusting pH value, and the mixed enzyme of oat quality 3-5% is added, stirs evenly, adjustment temperature
Degree is 65-70 DEG C, digests 50-80min, and for enzymolysis liquid through 100-300 mesh net filtration, filtrate is freeze-dried up to up to oat
Extract.The quality group of the mixed enzyme becomes: carbohydrase: pectase: cellulase=1:1-2:6-10.
The camellia seed method for preparing extractive are as follows: camellia seed is crushed to partial size 0.1-0.3mm, camellia seed quality is added
1-3 times of water is 5-6 with newborn acid for adjusting pH value, is warming up to 40-50 DEG C, and the mixed enzyme of camellia seed quality 3-5%, stirring is added
Uniformly, 55-80min is digested, for enzymolysis liquid through 100-300 mesh net filtration, filtrate is freeze-dried up to camellia seed extract;Institute
The quality group for stating mixed enzyme becomes: pectase: cellulase=1:6-10.
Aloe vera extract is the preparation method is as follows: take aloe barbadensis Miller in-18-- 23 DEG C of freezing 5-10min;Immediately
It is crushed to partial size 0.3-1mm;It sets in container and the sodium carbonate liquor for adding 2-3% adjusts pH value as 7-9, be warming up to 60-70 DEG C,
Heat preservation, the mixed enzyme that mixed material total weight 2-5% is then added digest 30-50min, and filtering obtains filtrate;Filtrate is cold through ultrafiltration
Jelly is drying to obtain.The mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:8-15:2 in mass ratio.
Grape seed extract is the preparation method is as follows: in-18-- 23 DEG C of freezing 30- after taking grape seed surface to spray lactic acid
50min;It is crushed to partial size 0.3-1mm immediately;It sets in container and the sodium carbonate liquor for adding 2-3% adjusts pH value as 7-9, heat up
To 60-70 DEG C, heat preservation, the mixed enzyme that mixed material total weight 2-5% is then added digests 30-50min, and filtering obtains filtrate;Filter
Liquid is freeze-dried after ultrafiltration to obtain the final product.The mixed enzyme is uniform by pectase, cellulase, amylase 1:8-15:2 in mass ratio
Mixing.
Ferment powder the preparation method is as follows:
It uses the fermentation liquid of pickle fermentation production latter stage discharge for raw material, is filtered to remove the big grain object such as dish leaf in fermentation liquid
Matter obtains fermented liquid, and fermented liquid prepares ferment powder using drying means such as freeze-dryings.
The condition of freeze-drying is: -30-40 degree pre-freeze 6 hours;Then it is lyophilized until water content is less than 5%.Pickle production
Method is prepared referring to Chinese patent 201110421967.3 or 201210310308.7.
Lactobacillus plantarum bacterium powder the preparation method is as follows:
Lactobacillus plantarum powdery bacterium powder, production stage are as follows: slant strains being transferred to fluid nutrient medium and are spread cultivation step by step
To the volume of requirement;The bacterium solution to spread cultivation is centrifuged, precipitating thallus is collected;Protective agent is added into precipitating thallus
And it is diluted;Powdery microbial inoculum is prepared using drying equipment, the above method is made viable count in lactobacillus plantarum bacterium powder and is
(0.1~9.0) * (1010) a/gram, starch can be added when mixing for bacterium powder and dextrin realizes the proper ratio of each bacterium viable count.Institute
Stating lactobacillus plantarum is CGMCC 11763.
The utility model has the advantages that
Camellia seed extract: oat extract: Jojoba seed extract: centella can be with compact epidermis and corium interconnecting piece
Point, skin can be made to limber up, help promotes collagen in skin corium to be formed, and has the effect for enabling skin compact, contains in ferment powder
There is lactic acid, vegetables water solubility nutritional ingredient vitamin, lactic acid bacteria, lactic acid bacteria can be metabolized generation lactic acid on facial mask.Water replenishment
With intensive care, skin acquisition is allowed purely to loosen and beautiful enjoyment.Filled moisture, the skin sufficiently soaked into burst forth smooth tender
With bright gloss, soft and moist careful care helps skin to resist external and internal pressure.Extraction contains Zhuo from beautiful graceful camellia
More moisturizing energy can help to restore skin and it is enabled to keep ideal water profit state.Have effects that maintenance, skin can be protected to exempt from
It is encroached on, and that strengthens skin self resists mechanism.
Specific embodiment
The lactobacillus plantarum is CGMCC 11763.
Lactobacillus plantarum CGMCC NO.11763 provided by the present invention is through experiments, it was found that the condition that can be 1.50 in pH
Lower survival, still in existing state after 1% cholate culture 4 hours;Lactobacillus plantarum CGMCC NO.11763 degradation nitrous acid
Salt speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and for the strain when producing pickles, nitrite is dense in entire fermentation process
Degree is in 4.8mg/kg or less;CGMCC NO.11763 can reach 64.76% to degrading rate of cholesterol after fermentation 60h hours.
CGMCC NO.11763 Adhering capacity measurement from agglutination rate be 95.71%.
CGMCC NO.11763 bacterial strain is to cholesterol degradation capability study and measurement:
1ml CGMCC NO.11763 mother liquor is taken to be inoculated in the MRS cholesterol fluid nutrient medium (cholesterol level of 10mL
0.1mg/ml, pH 6.2) in, culture 20h, 40h, 60h is spare respectively for 37 DEG C of constant temperature standing, to access the MRS of 1mL sterile water
Cholesterol culture medium is control, at taking the bacteria liquid sample of the above culture different time and comparison liquid 1ml, 9000r/min, 4 DEG C each
Be centrifuged 10min, obtain fermented supernatant fluid, o-phthalaldehyde method measure cholesterol level in supernatant (specifically: take each supernatant
For 0.1ml in corresponding test tube, the o-phthalaldehyde 0.15ml of acetic acid 0.3ml on the rocks, 1mg/ml are slowly added into the concentrated sulfuric acid
1.0ml is uniformly mixed.It is stored at room temperature 10min, surveys light absorption value under 550nm).3 repetitions of each processing, in kind make
Make cholesterol standard curve, calculates cholesterol level and degradation rate in supernatant, the results are shown in Table 1.It is found that CGMCC NO.11763
There is good degradation to cholesterol, after fermentation 60h hours, degradation rate can reach 64.76%.
Degradation situation of the table 1 to cholesterol.
Degradation time (h) |
0 |
20h |
40h |
60h |
Cholesterol level (mg/ml) |
0.2273±0.0058 |
0.1356±0.0018 |
0.1011±0.0094 |
0.801±0.0231 |
Degrading rate of cholesterol % |
|
40.34% |
55.52% |
64.76% |
The bile tolerance of CGMCC NO.11763 bacterial strain is tested:
Take CGMCC NO.11763 bacterium solution 1mL inoculation strain in containing different cholate (concentration gradients 0.0%, 0.2%,
0.4%, 0.6%, 0.8%, 1%) 10mL MRS fluid nutrient medium (PH=6.4), are placed at 37 DEG C and cultivate 0,2,4h respectively,
3 repetitions of each processing.It respectively takes 1ml sample bacterium solution to mix in 9ml physiological saline, prepares dilution solution, 0.1ml is taken to dilute
Liquid is coated in MRS, and it is flat that culture 48 hours (each dilution does 3 in parallel) record calculating is inverted in 37 DEG C of biochemical cultivation cases
Bacterium number number on plate.It the results are shown in Table 2.Know that bacterium increment of bacterium after gallbladder salinity is 1% processing 4h still reaches
0.59±0.92×10 7(cfu/ml), there is good bile tolerance ability.
2 bile tolerance ability of table detects [(± s) × 107cfu/ml]
The acid resistance test of CGMCC NO.11763 bacterial strain:
Take CGMCC NO.11763 mother liquor by 1ml inoculation strain in different pH value (pH gradient 1.5,2.0,2.5,3.0,
3.5,4.0) 10mL MRS fluid nutrient medium is placed at 37 DEG C and cultivates 0,2,4h respectively, 3 repetitions of each processing.Respectively take 1ml
Sample bacterium solution mixes in 9ml physiological saline, prepares dilute solution, and 0.1ml dilution is taken to be coated in MRS, in 37 DEG C of biochemistry
The bacterium colony number being inverted in incubator on culture 48 hours (each dilution does 3 in parallel) record plate.It the results are shown in Table 3.It says
The bright bacterium has very strong acid-fast ability.
3 acid-fast ability of table detects [(± s) × 107cfu/ml]
The Adhering capacity of CGMCC NO.11763 bacterial strain measures
Culture CGMCC NO.11763 (MRS fluid nutrient medium), 5 α of Escherichia coli DH (LB liquid medium) must be sent out for 24 hours
Zymotic fluid is respectively placed in 3000r/min, is centrifuged 10min at 4 DEG C, collect bacterium mud, uses the sterile phosphate buffer of pH=7.0 respectively
(PBS is added, concussion after mixing, is placed in 3000r/min, is centrifuged at 4 DEG C in liquid (PBS) washing bacterium mud 2 times in bacterium colony
10min collects thallus).From agglutination rate (%): bacterium mud CGMCC NO.11763 being formed at wavelength 600nm with sterile PBS
Light absorption value be 0.4 ± 0.1 (A 0) suspension bacteria liquid and bacteria suspension, stand for 24 hours afterwards measure light absorption value A 24, from agglutination rate
(%) formula is (A 0-A 24)/A 0.;His agglutination rate (%): by the outstanding bacterium solution of CGMCC NO.11763 and bacillus coli DH 5 alpha
It is adjusted to the mix suspending bacterium solution that the light absorption value at wavelength 600nm is 0.6 ± 0.1 (A 0).Light absorption value A is measured after standing 24H
24, his agglutination rate (%) formula is (A 0-A 24)/A 0.Measurement result is shown in Table 4, it is known that CGMCC NO.11763's is aggregated certainly
Rate is 95.71%, there is very strong Adhering capacity.
4 Adhering capacity table of table
Bacterial strain physiological property
It is micro- that lactobacillus plantarum (Lactobacillus plantarum) XH is preserved in China on November 30th, 2015
Biological inoculum preservation administration committee's common micro-organisms center (abbreviation CGMCC), deposit number are CGMCC NO.11763, preservation
Location are as follows: city of BeiJing, China Chaoyang District North Star West Road 1 institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.
The bacterial strain feature is as follows: it observes under the microscope, which is rod-short, and Gram's staining is positive, atrichia,
Do not produce gemma;On solid medium, which is white, and surface is smooth, fine and close, and form is circle, and edge is more neat.
Physicochemical characteristics are as follows: catalase (-), gelatin liquefaction (-), indoles test (+), motility (-), fermentation gas
(-), nitrate reductase (-), fermentation gas (-) produce hydrogen sulfide gas (-), grow (+) in pH4.0MRS culture medium.By
Physiology and biochemistry is accredited as lactobacillus plantarum (Lactobacillus plantarum), is named as lactobacillus plantarum
(Lactobacillus plantarum)XH。
The bacterial strain can at 57 DEG C well-grown, glucose tolerance 275g/L.
Lactobacillus plantarum of the present invention by acquire people Li Jianshu, from Xinjiang Uygur fellow-villager man in Yoghourt it is isolated,
Acquisition time on June 2nd, 2015.
5L fermentation tank test
(1) mono- ring of lactobacillus plantarum CGMCC NO.11763 on inclined-plane is taken, access is equipped with 50mL culture medium MRS (no fine jade
Rouge) (concentration of glucose 150g/L) culture medium 250mL triangular flask in, 200rpm, 37 DEG C of culture 12h or so make at thallus
In mid log phase.
(2) the strain access of logarithmic phase is fermented equipped with the 5L of 3L MRS fluid nutrient medium (initial glucose 150g/L)
In tank.Inoculum concentration is 10%, and 100rpm is cultivated 8 hours at 37 DEG C, logarithm dissolved oxygen early period control 10% (ventilation 0.5L/min), after
Phase Anaerobic culturel 63 hours.After fermentation, the lactic acid production of lactobacillus plantarum CGMCC NO.11763 reaches 110g/L.In this way
Galactopoiesis mollic acid be conducive to pickles Rapid Fermentation.
(4) by the strain access of logarithmic phase, equipped with 3L sodium nitrite liquid screening medium, (single nitrogen source is 2g/L nitrous
The modification MRS screening and culturing medium of sour sodium) 5L fermentor in.Inoculum concentration is 10%, and 100rpm is cultivated 8 hours at 37 DEG C, logarithm
Dissolved oxygen early period control 10% (ventilation 0.5L/min), later period anaerobism, fermentation process adds according to the wear rate stream of nitrite
The sodium nitrite solution of 20g/L is cultivated 2-3 days.After fermentation, fermentation process lactobacillus plantarum CGMCC NO.11763 is calculated
To the degradation rate of sodium nitrite.As a result, it has been found that: under this condition, XH can achieve 653mg/ to the degradation rate of sodium nitrite
h/L。
(5) the strain 10mL access of logarithmic phase is carried out equipped in 2kg pretreated Chinese cabbage according to conventional Kimchi method
Processing, the every 12 hours content of nitrite measured in pickles.As a result, it has been found that XH bacterium is to nitrous acid in entire fermentation process
The decomposition rate of sodium is 10.9mg/h/kg Chinese cabbage.Content of sodium nitrite in pickles is consistently lower than 4.8mg/kg, far below country
Content specified in standard GB2714-2003 (20mg/kg).
Embodiment 1
The present invention provides a kind of moisturizing, rehabilitation plant extractive composition and its application in cosmetics:
Moisturizing, the parts by weight composition of rehabilitation plant extractive composition are as follows:
Camellia seed extract 20, aloe vera extract 15, oat extract 10, Jojoba seed extract 12, accumulated snow
Careless extract 8, grape seed extract 3, ferment powder 6, lactobacillus plantarum bacterium powder 3.
The preparation method of the Gotu Kola P.E, which includes the following steps: to crush centella, to be placed on equipped with 0.4% carbon
In sour hydrogen sodium solution, the mass ratio of centella in the solution is 8 with newborn acid for adjusting pH value, is warming up to 45 DEG C in 10-30%,
The mixed enzyme of centella quality 1.5% is added, stirs evenly, 40min is digested, then in electric field strength 30kV/cm, burst length
It carries out high voltage electric field under the conditions of 500 μ s, pulse frequency 200-300Hz to handle 8 minutes, then room temperature 400W, 40KHz condition is ultrasonic
Handle 12min;The enzymolysis liquid of above-mentioned processing is drying to obtain Gotu Kola P.E through 200 mesh screen filter freezings;The mixed enzyme
Quality group become: carbohydrase: pectase: cellulase=1:3:7.
The preparation method of the Jojoba seed extract is the following steps are included: 5 times of its quality is added after crushing in Jojoba seed
Water, under the conditions of electric field strength 30kV/cm, 450 μ s of burst length, pulse frequency 200Hz carry out high voltage electric field extract 8 points
Clock;Adjusting pH value with sodium carbonate is 8, the compound enzyme of Jojoba seed quality 1.5% is added, in 52 DEG C of enzymatic hydrolysis 60min;Digest liquid cooling
It is 0.2mm up to Jojoba seed extract that dry, low-temperature grinding to partial size, which is lyophilized,;The compound enzyme be cellulase, zytase,
Laccase, pectase 6:8:2:1 in mass ratio are uniformly mixed.
The preparation method of the oat extract, include the following steps: by oat surface spray 3% lactic acid solution after in-
21-- 25 DEG C of freezing 30min;It is crushed immediately, partial size 0.5mm;4 times of crushed material quality of deionized water, mixing is added
Uniformly, it is 6 with lemon acid for adjusting pH value, the mixed enzyme of oat quality 4% is added, stirs evenly, adjustment temperature is 65-70 DEG C,
70min is digested, for enzymolysis liquid through 200 mesh net filtrations, filtrate is freeze-dried up to up to oat extract.The mixed enzyme
Quality group becomes: carbohydrase: pectase: cellulase=1:1:8.
The camellia seed method for preparing extractive are as follows: camellia seed is crushed to partial size 0.2mm, is added 2 times of camellia seed quality
Water, be 5 with newborn acid for adjusting pH value, be warming up to 45 DEG C, the mixed enzyme of camellia seed quality 4% is added, stirs evenly, digest
60min, for enzymolysis liquid through 200 mesh net filtrations, filtrate is freeze-dried up to camellia seed extract;The quality group of the mixed enzyme
Become: pectase: cellulase=1:8.
Aloe vera extract is the preparation method is as follows: take aloe barbadensis Miller in-18-- 23 DEG C of freezing 5-10min;Immediately
It is crushed to partial size 0.3-1mm;It sets in container and the sodium carbonate liquor for adding 2-3% adjusts pH value as 7-9, be warming up to 60-70 DEG C,
Heat preservation, the mixed enzyme that mixed material total weight 2-5% is then added digest 30-50min, and filtering obtains filtrate;Filtrate is cold through ultrafiltration
Jelly is drying to obtain.The mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:8-15:2 in mass ratio.
Grape seed extract is the preparation method is as follows: in -23 DEG C of freezing 50min after grape seed surface sprinkling lactic acid;Powder immediately
It is broken to partial size 0.5mm;Set in container and add 2% sodium carbonate liquor to adjust pH value be 8, be warming up to 65 DEG C, heat preservation, then plus
Enter the mixed enzyme enzymatic hydrolysis 40min of mixed material total weight 3%, filtering obtains filtrate;Filtrate is freeze-dried after ultrafiltration to obtain the final product.Institute
Mixed enzyme is stated uniformly to be mixed by pectase, cellulase, amylase 1:10:2 in mass ratio.
Ferment powder the preparation method is as follows:
It uses the fermentation liquid of pickle fermentation production latter stage discharge for raw material, is filtered to remove the big grain object such as dish leaf in fermentation liquid
Matter obtains fermented liquid, and fermented liquid prepares ferment powder using drying means such as freeze-dryings.
The condition of freeze-drying is: -30 DEG C pre-freeze 6 hours;Then it is lyophilized until water content is less than 5%.Pickle production side
Method is prepared referring to Chinese patent 201110421967.3 or 201210310308.7.
Lactobacillus plantarum bacterium powder the preparation method is as follows:
Lactobacillus plantarum powdery bacterium powder, production stage are as follows: slant strains being transferred to fluid nutrient medium and are spread cultivation step by step
To the volume of requirement;The bacterium solution to spread cultivation is centrifuged, precipitating thallus is collected;Protective agent is added into precipitating thallus
And it is diluted;Powdery microbial inoculum is prepared using drying equipment, it is (8 that viable count in lactobacillus plantarum bacterium powder, which is made, in the above method
~9.0) (10 *10) a/gram, starch can be added when mixing for bacterium powder and dextrin realizes the proper ratio of each bacterium viable count.The plant
Object lactobacillus is CGMCC 11763.
The lactobacillus plantarum is CGMCC 11763.
Embodiment 2 is substantially the same as example 1
The present invention provides a kind of moisturizing, rehabilitation plant extractive composition and its application in cosmetics:
Moisturizing, the parts by weight composition of rehabilitation plant extractive composition are as follows:
Camellia seed extract 15, aloe vera extract 20, oat extract 8, Jojoba seed extract 15, centella
Extract 10, grape seed extract 2, ferment powder 8, lactobacillus plantarum bacterium powder 2.
The preparation method of the Gotu Kola P.E, which includes the following steps: to crush centella, to be placed on equipped with 0.3% carbon
In sour hydrogen sodium solution, it is 7 with newborn acid for adjusting pH value, is warming up to 40-50 DEG C, the mixed enzyme of centella quality 1-2%, stirring is added
Uniformly, 35min is digested, under the conditions of electric field strength 20-40kV/cm, burst length 400-500 μ s, pulse frequency 200-300Hz
It carries out high voltage electric field to handle 10 minutes, then room temperature 400W, 40KHz condition is ultrasonically treated 10min;The enzymolysis liquid of above-mentioned processing passes through
100-300 mesh screen filter freezing is drying to obtain Gotu Kola P.E;The quality group of the mixed enzyme becomes: carbohydrase: pectin
Enzyme: cellulase=1:5:10.
The preparation method of the Jojoba seed extract is the following steps are included: 7 times of its quality is added after crushing in Jojoba seed
Water, carry out high-voltage electricity under the conditions of electric field strength 20-40kV/cm, burst length 400-500 μ s, pulse frequency 200-300Hz
It extracts 10 minutes field;Adjusting pH value with sodium carbonate is 7, the compound enzyme of Jojoba seed quality 1% is added, in 48 DEG C of enzymatic hydrolysis 50min;
Enzymolysis liquid freeze-drying, low-temperature grinding to partial size are 0.1mm up to Jojoba seed extract;The compound enzyme be cellulase,
Zytase, laccase, pectase 6:10:2:1 in mass ratio are uniformly mixed.
The preparation method of the oat extract, include the following steps: by oat surface spray 2% lactic acid solution after in-
21-- 25 DEG C of freezing 10min;It is crushed immediately, partial size 1mm;3 times of crushed material quality of deionized water is added, mixing is equal
It is even, it is 5 with lemon acid for adjusting pH value, the mixed enzyme of oat quality 3% is added, stirs evenly, adjustment temperature is 65-70 DEG C, enzyme
80min is solved, for enzymolysis liquid through 100-300 mesh net filtration, filtrate is freeze-dried up to up to oat extract.The mixed enzyme
Quality group become: carbohydrase: pectase: cellulase=1:2:6.
The camellia seed method for preparing extractive are as follows: camellia seed is crushed to partial size 0.1-0.3mm, camellia seed quality is added
3 times of water is 5 with newborn acid for adjusting pH value, is warming up to 40 DEG C, the mixed enzyme of camellia seed quality 3% is added, stirs evenly, digests
70min, for enzymolysis liquid through 100-300 mesh net filtration, filtrate is freeze-dried up to camellia seed extract;The matter of the mixed enzyme
Amount composition are as follows: pectase: cellulase=1:6.
Aloe vera extract is the preparation method is as follows: take aloe barbadensis Miller in-18-- 23 DEG C of freezing 10min;Powder immediately
It is broken to partial size 1mm;The sodium carbonate liquor adjusting pH value for setting in container and adding 2% is 9, is warming up to 60-70 DEG C, keeps the temperature, then
The mixed enzyme that mixed material total weight 2% is added digests 50min, and filtering obtains filtrate;Filtrate is freeze-dried through ultrafiltration to obtain the final product.Institute
Mixed enzyme is stated uniformly to be mixed by pectase, cellulase, amylase 1:10:2 in mass ratio.
Grape seed extract is the preparation method is as follows: in-18-- 23 DEG C of freezing 50min after taking grape seed surface to spray lactic acid;
It is crushed to partial size 0.3mm immediately;The sodium carbonate liquor adjusting pH value for setting in container and adding 2% is 7, is warming up to 70 DEG C, keeps the temperature,
Then the mixed enzyme that mixed material total weight 2% is added digests 50min, and filtering obtains filtrate;Filtrate is freeze-dried after ultrafiltration
?.The mixed enzyme is uniformly mixed by pectase, cellulase, amylase 1:15:2 in mass ratio.
Ferment powder the preparation method is as follows:
It uses the fermentation liquid of pickle fermentation production latter stage discharge for raw material, is filtered to remove the big grain object such as dish leaf in fermentation liquid
Matter obtains fermented liquid, and fermented liquid prepares ferment powder using drying means such as freeze-dryings.
The condition of freeze-drying is: -30 degree pre-freeze 6 hours;Then it is lyophilized until water content is less than 5%.Pickle production side
Method is prepared referring to Chinese patent 201110421967.3 or 201210310308.7.
The lactobacillus plantarum is CGMCC 11763.
Embodiment 3
The present invention provides a kind of moisturizing, rehabilitation plant extractive composition and its application in cosmetics:
Moisturizing, the parts by weight composition of rehabilitation plant extractive composition are as follows:
Camellia seed extract 30, aloe vera extract 10, oat extract 8, Jojoba seed extract 15, centella
Extract 5, grape seed extract 2, ferment powder 5, lactobacillus plantarum bacterium powder 2.
Using effect:
Facial mask effect test: GB15979-2002 standard, little white rabbit skin detection are non-stimulated substance.
Moisture-keeping efficacy: referring to QB/T 4256-2011 " cosmetics moisture-keeping efficacy evaluation guide ".
This comparative test is referring to " cosmetics moisture-keeping efficacy evaluation guide " (QB/T4256-2011), in constant temperature and humidity ring
After mask is intended for single use by volunteer in border, moistening effect of the non-invasive evaluation mask of instrument in 6 hours is utilized.It surveys
During examination, test mask and blank control are randomly distributed in the left and right inner forearm of subject.Product is intended for single use, patch
Applying the time is 20 minutes.Respectively after product is used 0.5 hour, 1 hour, 2 hours, 4 hours and 6 hours using preceding and product,
Keratoderma moisture is carried out respectively using area and blank district to product using moisture of skin tester (Corneometer)
Content measuring.Film sample has 50 ginseng adductions to complete test.Product is tested with half an hour after, and skin moisture content is average
48.56 units are increased, it is highest to reach 55 units.
Using the mask product for the composition application preparation combined in list, chooses 196 people and carry out effect test, produce
Product effect is shown in Table 5.
Plant composition ingredient adds in 5 mask product of table
Remarks: camellia seed extract (A), aloe vera extract (B), Jojoba seed extract (C), centella are extracted
Object (D), grape seed extract (E), oat extract (F), ferment powder (G), lactobacillus plantarum (H), whitening (X), skin elasticity
(W), skin complexion improves (Z).
The addition measurement ratio median addition of plant composition ingredient is added in product;38,39 is using examples 1 in list,
The mask product of plant composition in example 2, other are the mask product effect that different plant compositions are added according to table.