CN106389161A - Preparation method of cosmetic component with moisturizing efficacy - Google Patents
Preparation method of cosmetic component with moisturizing efficacy Download PDFInfo
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- CN106389161A CN106389161A CN201610860288.9A CN201610860288A CN106389161A CN 106389161 A CN106389161 A CN 106389161A CN 201610860288 A CN201610860288 A CN 201610860288A CN 106389161 A CN106389161 A CN 106389161A
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- ethanol
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- cocoa butter
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/007—Preparations for dry skin
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
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- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a preparation method of a cosmetic component with moisturizing efficacy. The cosmetic component is characterized by comprising the following main subcomponents: aloes and cacao beans. The preparation method comprises the following steps: mixing the aloes and the cacao beans according to a ratio of 1:1, adding water of which the mass is 10-12 times of the mass of the mixture to prepare extraction solution, adding cellulase and pectase into the extraction solution, performing hot water extraction, performing 81% alcohol precipitation, and decolorizing, namely proportionally adding water of which the mass is 10-12 times of the mass of the mixture, 1.1-2.2% of cellulase and 0.5-1.2% of pectase, performing hot water extraction twice, performing alcohol precipitation for three times, and decolorizing twice to obtain the cosmetic component. The extracting process provided by the invention has the characteristics of high efficiency, high extracting rate and stable extract component, and under the optimum extracting process condition, the extracting rate is as high as 9.93%.
Description
Technical field
The present invention relates to cosmetic technical field is and in particular to a kind of preparation side of the components of cosmetics with moisture-keeping efficacy
Method.
Background technology
Aloe is the four seasons raw liliaceous plant that a kind of juice enriches, and contains the composition improving skin in its leaf juice.
Aloe originates in Africa, can grow in temperate zone all over the world and torrid areas.Its blade gel is rich in beneficial polysaccharide molecule and ammonia
Base acid, is used for skin of releiving always over 2500, alleviate xerosis cutiss and allergic problem；Cocoa butter is a kind of from cacao bean
The wax component extracting, have functions that natural moisten, moisturizing and softening skin, at present, the plant component that contains in cosmetics
Extracting method mainly have Hot water extraction, acidleach lifting manipulation, alkali method, ultrasonic extraction and microwave loss mechanisms etc., these
All there is certain deficiency in method, as relatively low in extraction ratio, purity is not high, or the too high destruction active polysaccharide of Extracting temperature, or
Degraded of polysaccharide molecule etc. can be caused.
Content of the invention
The technical problem to be solved is to provide a kind of preparation method of the components of cosmetics with moisture-keeping efficacy.
For realizing the purpose of the present invention, the technical solution adopted in the present invention is：
A kind of preparation method of the components of cosmetics with moisture-keeping efficacy, raw material comprises following main component：Aloe and cacao bean,
Add 10-12 times of water configuration extraction liquid by mixing, in extracting solution, add cellulase and pectase hot water extraction,
81% precipitate with ethanol, the step such as depigmentation refines and forms, and concrete preparation method is as follows：(1) hot water extraction：Aloe and cacao bean are pressed 1:1
Mixing is pulverized, and crosses 50 mesh sieves, adds the water of 10-12 times of quality to be configured to extracting solution；Adjusting pH with phosphate buffer is 6.8,
The cellulase of 1.1-2.2% and the pectase of 0.5-1.2% is added to mix, 45-55 DEG C of stirring in water bath heating extraction 55 minutes,
Sucking filtration obtains filtrate；Filtering residue is added the water of 10-12 times of quality, adjusting pH with phosphate buffer is 6.8, adds 1.1-2.2%
Cellulase and the pectase of 0.5-1.2% mix, 45-55 DEG C of stirring in water bath heating extraction extract once for 55 minutes again, sucking filtration
Obtain filtrate, merge filtrate twice, filtrate is carried out with 90 DEG C of inactivators 10 minutes；
(2) concentrate：Concentrating filtrate to concentration is 0.5-1g/ml, puts to room temperature；
(3) first time precipitate with ethanol：95.2% ethanol to determining alcohol is added to be 81.5% in concentrated solution, stirring while adding, closure
Sternly, stand 24-48 hour；Sucking filtration obtains Aloe polysaccharide and cocoa butter composition；
(4) second precipitate with ethanol：To step（3）95.2% ethanol to determining alcohol is added to be 81.5% in prepared liquid, side edged
Stirring, sealing covers tightly, and stands 24-48 hour；Sucking filtration, with absolute ethanol washing 2-3 time, vacuum drying, obtains Aloe polysaccharide and can
Can fat composition；
(6) depigmentation：By Aloe polysaccharide and the appropriate water dissolution of cocoa butter composition, the activated carbon of weight 1.1% is added to mix, plus
Heat stirs 25 minutes to 81 DEG C of temperature, filters to take filtrate；The activated carbon that above-mentioned filtrate is added weight 1.1% mixes, and is heated to
81 DEG C of temperature stir 25 minutes, filter, obtain depigmentation Aloe polysaccharide and cocoa butter ingredient solution；
(7) third time precipitate with ethanol：Depigmentation Aloe polysaccharide and cocoa butter composition liquid addition 95.2% ethanol to determining alcohol are
81.5%, stirring while adding, sealing covers tightly, and stands 24 hours；Sucking filtration, with absolute ethanol washing 2-3 time, vacuum drying, obtains smart
Aloe polysaccharide processed and cocoa butter composition.
Preferably, described preparation method is as follows：(1) take Aloe 10g and cacao bean 10g mixing to be pulverized, cross 50 mesh
Sieve, adds the water of 220ml to be configured to extracting solution；Adjusting pH with phosphate buffer is 6.8, adds 1.8% cellulase and 0.8%
Pectase mix, 50 DEG C of stirring in water bath heating extraction 55 minutes, sucking filtration obtains filtrate；Filtering residue is added the water of 220ml, use sulphuric acid
It is 6.8 that buffer adjusts pH, adds 1.8% cellulase and 0.8% pectase mixes, 50 DEG C of stirring in water bath heating carry
Take extract again once within 55 minutes, sucking filtration obtains filtrate, merge filtrate twice, filtrate is carried out with 90 DEG C of inactivators 10 minutes；
(2) concentrate：Concentrating filtrate to concentration is 35-50ml, puts to room temperature；
(3) first time precipitate with ethanol：95.2% ethanol to determining alcohol is added to be 81.5% in concentrated solution, stirring while adding, closure
Sternly, 25 hours are stood；Sucking filtration obtains Aloe polysaccharide and cocoa butter composition；
(4) second precipitate with ethanol：To step（3）95.2% ethanol to determining alcohol is added to be 81.5% in prepared liquid, side edged
Stirring, sealing covers tightly, and stands 25 hours；Sucking filtration, the absolute ethanol washing of 3-5 times of addition 2 times, vacuum drying, obtain Aloe polysaccharide
With cocoa butter composition；
(6) the first time depigmentation：By Aloe polysaccharide and the appropriate water dissolution of cocoa butter composition, add the activated carbon of weight 1.1%
Mix, be heated to 81 DEG C of temperature and stir 25 minutes, filter to take filtrate；
(7) second depigmentation：The activated carbon that above-mentioned filtrate is added weight 1.1% mixes, and is heated to 81 DEG C of temperature stirrings 25
Minute, filter, obtain depigmentation Aloe polysaccharide and cocoa butter ingredient solution；
(8) third time precipitate with ethanol：Depigmentation Aloe polysaccharide and cocoa butter composition liquid addition 95.2% ethanol to determining alcohol are
81.5%, stirring while adding, sealing covers tightly, and stands 24 hours；Sucking filtration, with absolute ethanol washing 2-3 time, vacuum drying, obtains smart
Aloe polysaccharide processed and cocoa butter composition.
A kind of preparation method of components of cosmetics with moisture-keeping efficacy that the present invention provides, using Aloe and cacao bean be
Main component refines and forms, apply to have in cosmetics skin of releiving, alleviate xerosis cutiss and allergic problem, natural moisten,
Moisturizing and effect of softening skin, and there is no toxic action to skin, this cosmetics plant is extracted using gentle enzymatic isolation method
Composition, not only will not destroy the structure of composition itself, improve extraction ratio simultaneously, and can be used for industrial metaplasia on a large scale
Produce, there is certain market prospect and economic benefit.
Specific embodiment
The following enforcement to the present invention is further described, so that those skilled in the art can be better understood from simultaneously
Implement.
Embodiment 1
(1) take Aloe 10g and cacao bean 10g mixing to be pulverized, cross 50 mesh sieves, plus 200g water is configured to extracting solution；Use sulphuric acid
PH adjusted by buffer is 6.8, adds 2.2% cellulase and 1.2% pectase to mix, 55 DEG C of stirring in water bath heating extraction 55
Minute, sucking filtration obtains filtrate；Filtering residue is added the water of 200g, adjusting pH with phosphate buffer is 6.8, adds 2.2% fiber
Plain enzyme and 1.2% pectase mix, and 55 DEG C of stirring in water bath heating extraction extract once for 55 minutes again, and sucking filtration obtains filtrate, merge
Filtrate twice, carries out 90 DEG C of inactivators 10 minutes to filtrate；
(2) concentrate：Concentrating filtrate to concentration is 30-45ml, puts to room temperature；
(3) first time precipitate with ethanol：95.2% ethanol to determining alcohol is added to be 81.5% in concentrated solution, stirring while adding, closure
Sternly, 24 hours are stood；Sucking filtration obtains Aloe polysaccharide and cocoa butter composition；
(4) second precipitate with ethanol：To step（3）95.2% ethanol to determining alcohol is added to be 81.5% in prepared liquid, side edged
Stirring, sealing covers tightly, and stands 24 hours；Sucking filtration, with absolute ethanol washing 2 times, vacuum drying, obtains Aloe polysaccharide and becomes with cocoa butter
Point；
(6) depigmentation：By Aloe polysaccharide and the appropriate water dissolution of cocoa butter composition, the activated carbon of weight 1.1% is added to mix, plus
Heat stirs 25 minutes to 81 DEG C of temperature, filters to take filtrate；The activated carbon that above-mentioned filtrate is added weight 1.1% mixes, and is heated to
81 DEG C of temperature stir 25 minutes, filter, obtain depigmentation Aloe polysaccharide and cocoa butter ingredient solution；
(7) third time precipitate with ethanol：Depigmentation Aloe polysaccharide and cocoa butter composition liquid addition 95.2% ethanol to determining alcohol are
81.5%, stirring while adding, sealing covers tightly, and stands 24 hours；Sucking filtration, with absolute ethanol washing 2 times, vacuum drying, obtains refined
Aloe polysaccharide and cocoa butter composition.
Embodiment 2
(1) take Aloe 10g and cacao bean 10g mixing to be pulverized, cross 50 mesh sieves, add the water of 220ml to be configured to extracting solution；With
It is 6.8 that phosphate buffer adjusts pH, adds 1.8% cellulase and 0.8% pectase to mix, 50 DEG C of stirring in water bath heating carry
Take 55 minutes, sucking filtration obtains filtrate；Filtering residue is added the water of 220ml, adjusting pH with phosphate buffer is 6.8, adds 1.8%
Cellulase and 0.8% pectase mix, and 50 DEG C of stirring in water bath heating extraction extract once for 55 minutes again, and sucking filtration obtains filtrate, close
And filtrate twice, filtrate is carried out with 90 DEG C of inactivators 10 minutes；
(2) concentrate：Concentrating filtrate to concentration is 35-50ml, puts to room temperature；
(3) first time precipitate with ethanol：95.2% ethanol to determining alcohol is added to be 81.5% in concentrated solution, stirring while adding, closure
Sternly, 25 hours are stood；Sucking filtration obtains Aloe polysaccharide and cocoa butter composition；
(4) second precipitate with ethanol：To step（3）95.2% ethanol to determining alcohol is added to be 81.5% in prepared liquid, side edged
Stirring, sealing covers tightly, and stands 25 hours；Sucking filtration, adds 3 times of absolute ethanol washing 2 times, vacuum drying, obtain Aloe polysaccharide and
Cocoa butter composition；
(6) the first time depigmentation：By Aloe polysaccharide and the appropriate water dissolution of cocoa butter composition, add the activated carbon of weight 1.1%
Mix, be heated to 81 DEG C of temperature and stir 25 minutes, filter to take filtrate；
(7) second depigmentation：The activated carbon that above-mentioned filtrate is added weight 1.1% mixes, and is heated to 81 DEG C of temperature stirrings 25
Minute, filter, obtain depigmentation Aloe polysaccharide and cocoa butter ingredient solution；
(8) third time precipitate with ethanol：Depigmentation Aloe polysaccharide and cocoa butter composition liquid addition 95.2% ethanol to determining alcohol are
81.5%, stirring while adding, sealing covers tightly, and stands 24 hours；Sucking filtration, with absolute ethanol washing 2 times, vacuum drying, obtains refined
Aloe polysaccharide and cocoa butter composition.
Claims (2)
1. a kind of preparation method of the components of cosmetics with moisture-keeping efficacy is it is characterised in that raw material comprises following main component：
Aloe and cacao bean, add 10-12 times of water configuration extraction liquid by mixing, add cellulase and fruit in extracting solution
Glue enzyme hot water extraction, 81% precipitate with ethanol, the step such as depigmentation refines and forms, and concrete preparation method is as follows：(1) hot water extraction：By Aloe
Press 1 with cacao bean:1 mixing is pulverized, and crosses 50 mesh sieves, adds the water of 10-12 times of quality to be configured to extracting solution；Buffered with sulphuric acid
It is 6.8 that liquid adjusts pH, adds the cellulase of 1.1-2.2% and the pectase of 0.5-1.2% to mix, 45-55 DEG C of stirring in water bath heating
Extract 55 minutes, sucking filtration obtains filtrate；Filtering residue is added the water of 10-12 times of quality, adjusting pH with phosphate buffer is 6.8, then plus
The pectase of the cellulase and 0.5-1.2% that enter 1.1-2.2% mixes, and 45-55 DEG C of stirring in water bath heating extraction carries for 55 minutes again
Take once, sucking filtration obtains filtrate, merge filtrate twice, filtrate is carried out with 90 DEG C of inactivators 10 minutes；
(2) concentrate：Concentrating filtrate to concentration is 0.5-1g/ml, puts to room temperature；
(3) first time precipitate with ethanol：95.2% ethanol to determining alcohol is added to be 81.5% in concentrated solution, stirring while adding, closure
Sternly, stand 24-48 hour；Sucking filtration obtains Aloe polysaccharide and cocoa butter composition；
(4) second precipitate with ethanol：To step（3）95.2% ethanol to determining alcohol is added to be 81.5% in prepared liquid, side edged
Stirring, sealing covers tightly, and stands 24-48 hour；Sucking filtration, with absolute ethanol washing 2-3 time, vacuum drying, obtains Aloe polysaccharide and can
Can fat composition；
(6) depigmentation：By Aloe polysaccharide and the appropriate water dissolution of cocoa butter composition, the activated carbon of weight 1.1% is added to mix, plus
Heat stirs 25 minutes to 81 DEG C of temperature, filters to take filtrate；The activated carbon that above-mentioned filtrate is added weight 1.1% mixes, and is heated to
81 DEG C of temperature stir 25 minutes, filter, obtain depigmentation Aloe polysaccharide and cocoa butter ingredient solution；
(7) third time precipitate with ethanol：Depigmentation Aloe polysaccharide and cocoa butter composition liquid addition 95.2% ethanol to determining alcohol are
81.5%, stirring while adding, sealing covers tightly, and stands 24 hours；Sucking filtration, with absolute ethanol washing 2-3 time, vacuum drying, obtains smart
Aloe polysaccharide processed and cocoa butter composition.
2. a kind of components of cosmetics with moisture-keeping efficacy according to claim 1 preparation method it is characterised in that：
(1) take Aloe 10g and cacao bean 10g mixing to be pulverized, cross 50 mesh sieves, add the water of 220ml to be configured to extracting solution；Use sulphuric acid
PH adjusted by buffer is 6.8, adds 1.8% cellulase and 0.8% pectase to mix, 50 DEG C of stirring in water bath heating extraction 55
Minute, sucking filtration obtains filtrate；Filtering residue is added the water of 220ml, adjusting pH with phosphate buffer is 6.8, adds 1.8% fiber
Plain enzyme and 0.8% pectase mix, and 50 DEG C of stirring in water bath heating extraction extract once for 55 minutes again, and sucking filtration obtains filtrate, merge two
Secondary filtrate, carries out 90 DEG C of inactivators 10 minutes to filtrate；
(2) concentrate：Concentrating filtrate to concentration is 35-50ml, puts to room temperature；
(3) first time precipitate with ethanol：95.2% ethanol to determining alcohol is added to be 81.5% in concentrated solution, stirring while adding, closure
Sternly, 25 hours are stood；Sucking filtration obtains Aloe polysaccharide and cocoa butter composition；
(4) second precipitate with ethanol：To step（3）95.2% ethanol to determining alcohol is added to be 81.5% in prepared liquid, side edged
Stirring, sealing covers tightly, and stands 25 hours；Sucking filtration, the absolute ethanol washing of 3-5 times of addition 2 times, vacuum drying, obtain Aloe polysaccharide
With cocoa butter composition；
(6) the first time depigmentation：By Aloe polysaccharide and the appropriate water dissolution of cocoa butter composition, add the activated carbon of weight 1.1%
Mix, be heated to 81 DEG C of temperature and stir 25 minutes, filter to take filtrate；
(7) second depigmentation：The activated carbon that above-mentioned filtrate is added weight 1.1% mixes, and is heated to 81 DEG C of temperature stirrings 25
Minute, filter, obtain depigmentation Aloe polysaccharide and cocoa butter ingredient solution；
(8) third time precipitate with ethanol：Depigmentation Aloe polysaccharide and cocoa butter composition liquid addition 95.2% ethanol to determining alcohol are
81.5%, stirring while adding, sealing covers tightly, and stands 24 hours；Sucking filtration, with absolute ethanol washing 2-3 time, vacuum drying, obtains smart
Aloe polysaccharide processed and cocoa butter composition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610860288.9A CN106389161A (en) | 2016-09-29 | 2016-09-29 | Preparation method of cosmetic component with moisturizing efficacy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610860288.9A CN106389161A (en) | 2016-09-29 | 2016-09-29 | Preparation method of cosmetic component with moisturizing efficacy |
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| CN106389161A true CN106389161A (en) | 2017-02-15 |
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| CN201610860288.9A Withdrawn CN106389161A (en) | 2016-09-29 | 2016-09-29 | Preparation method of cosmetic component with moisturizing efficacy |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108309873A (en) * | 2018-05-07 | 2018-07-24 | 深圳润邦之家生物科技有限公司 | Assign balanced conditioning Essence living |
| CN109320626A (en) * | 2018-10-08 | 2019-02-12 | 伍曾利 | A kind of aloe polysaccharide and the preparation method and application thereof |
| CN111419743A (en) * | 2020-03-03 | 2020-07-17 | 齐鲁工业大学 | Preparation method and application of vitis amurensis extract |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104825351A (en) * | 2015-04-24 | 2015-08-12 | 东莞波顿香料有限公司 | Eaglewood whitening facial cleanser and preparation method thereof |
| CN105902476A (en) * | 2016-03-11 | 2016-08-31 | 千草万木（北京）科技有限公司 | Moisture retention and regeneration plant extract composition and its use in cosmetic |
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2016
- 2016-09-29 CN CN201610860288.9A patent/CN106389161A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104825351A (en) * | 2015-04-24 | 2015-08-12 | 东莞波顿香料有限公司 | Eaglewood whitening facial cleanser and preparation method thereof |
| CN105902476A (en) * | 2016-03-11 | 2016-08-31 | 千草万木（北京）科技有限公司 | Moisture retention and regeneration plant extract composition and its use in cosmetic |
Non-Patent Citations (3)
| Title |
|---|
| 李亚辉 等: "响应面法优化复合酶提取芦荟多糖工艺及其抗氧化活性分析", 《食品科学（工艺技术）》 * |
| 王建新: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
| 王振宇: "《食用植物资源学—食用植物开发利用原理》", 30 March 2004, 东北林业大学出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108309873A (en) * | 2018-05-07 | 2018-07-24 | 深圳润邦之家生物科技有限公司 | Assign balanced conditioning Essence living |
| CN108309873B (en) * | 2018-05-07 | 2021-04-13 | 深圳润邦之家生物科技有限公司 | Activation-balanced conditioning essence |
| CN109320626A (en) * | 2018-10-08 | 2019-02-12 | 伍曾利 | A kind of aloe polysaccharide and the preparation method and application thereof |
| CN109320626B (en) * | 2018-10-08 | 2020-06-23 | 伍曾利 | Aloe polysaccharide and preparation method and application thereof |
| CN111419743A (en) * | 2020-03-03 | 2020-07-17 | 齐鲁工业大学 | Preparation method and application of vitis amurensis extract |
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