CN109608514A - The method of protein and polysaccharide is extracted from turnip - Google Patents
The method of protein and polysaccharide is extracted from turnip Download PDFInfo
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- CN109608514A CN109608514A CN201811439745.2A CN201811439745A CN109608514A CN 109608514 A CN109608514 A CN 109608514A CN 201811439745 A CN201811439745 A CN 201811439745A CN 109608514 A CN109608514 A CN 109608514A
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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Abstract
The present invention relates to the methods that protein and polysaccharide are extracted from turnip, comprising the following steps: slurries are made in turnip mashing, ultrasound by pretreatment；Enzymatic hydrolysis is added glusulase and iodoacetic acid simultaneously to slurries, and adjusts pH to faintly acid；Heating, faintly acid slurries are slowly heated, then are cooled to room temperature；The cooling pulp centrifuged processing finished is obtained clear liquid by centrifugation；It saltouts, again the pH of adjusting clear liquid to faintly acid, and under preference temperature, the mixed solution of ammonium sulfate saturated solution and sodium sulphate saturated solution is added, stirs evenly and places layering；Removal salt of repeatedly dialysing under lower sediment liquid certain temperature after layering is obtained turnip protein, supernatant is dried to obtain rutabaga polysaccharide by the extraction of protein and polysaccharide.The present invention is to extract the simple and easy method of protein and polysaccharide simultaneously from turnip, and the average recoveries of protein, up to 5.5% or more, the average recoveries of polysaccharide are up to 7.9% or more.
Description
Technical field
The invention belongs to biomaterial extractive technique fields, in particular to the side of protein and polysaccharide is extracted from turnip
Method.
Background technique
Turnip is the root or leaf of Cruciferae Brassica plants turnip (BrassicarapaL.), also known as turnip, is common
Edible vegetable, it is acrid flavour, sweet, bitter, it is warm-natured, return stomach, Liver Channel, can help digestion lower gas, removing toxicity for detumescence.Modern studies have found that turnip
In contain the organic acids such as protein, polysaccharide, fat, calcium, phosphorus, riboflavin, niacin, vitamin and caffeic acid, ferulic acid
Object is closed, it has the effects of antibacterial, anti parasitic, inhibition thyroxine synthesis, anti-distortion, anti-aging.Turnip huge number,
The produced turnip ingredient in various regions is not often identical, although there are many effective component type in turnip, is not yet fully used, and lacks
The method of weary comprehensive utilization.
In terms of the research extracted at present to turnip is mainly concentrated with rutabaga polysaccharide and turnip water extract.It has been investigated that climing
Cyanines protein and polyoses content are high, and the inside contains that there are many effective components for inhibiting thyroxine synthesis and anti-distortion, but by this
The method of two kinds of compositions while high efficiency extraction there is no people to study.
Summary of the invention
The purpose of the present invention is in order to solve the above problem, the present invention provides a kind of can extract polysaccharide and albumen in turnip simultaneously
Two kinds of beneficiating ingredients are realized using simple method while being extracted to the method for matter, realize the full fruit comprehensive utilization of turnip.
According to an aspect of the present invention, providing a kind of method that protein and polysaccharide are extracted from turnip includes following step
Rapid: slurries are made in turnip mashing, ultrasound by pretreatment；Enzymatic hydrolysis is added glusulase and iodoacetic acid simultaneously to slurries, and adjusts
PH is to faintly acid；Heating, faintly acid slurries are slowly heated, then are cooled to room temperature；Centrifugation finishes cooling pulp centrifuged
Processing obtains clear liquid；It saltouts, again the pH of adjusting clear liquid to faintly acid, and under preference temperature, ammonium sulfate and sodium sulphate is added
Mixed solution, stir evenly and place layering；The extraction of protein and polysaccharide repeatedly dialyses the lower sediment liquid after layering
Salt is removed, turnip protein is obtained, supernatant is dried to obtain rutabaga polysaccharide.
Wherein, in enzymolysis step, the pH value of slurries is adjusted to 5~7, and the quality proportioning of glusulase and turnip is 1:300-
500, the quality proportioning of iodoacetic acid and turnip is 1:800~1500.
Wherein, in salting-out step, the ratio of ammonium sulfate and metabisulfite solution is 1:1-4 in mixed solution, is mixed molten
Liquid and the mass ratio of turnip are 1:45~60.
Wherein, in salting-out step, the pH value of supernatant is adjusted to 4~7, the environment that the suitable temperature is 20~40 degree
Temperature；The placement separation time is 12~20 hours.
Wherein, the multiple dialysis is that lower sediment liquid is dialysed 4~6 times in three hydrogen aminomethane buffers.
Wherein, three hydrogen aminomethane buffer concentrations are 0.02mol/L-0.10mol/L, and the dialysis uses saturating
The retention molecule for analysing bag is 10-40kDa.
Wherein, the heating temperature of heating stepses is 40~70 DEG C, and heating time is 30-50 minute, the cooling time for 8~
13 hours.
Wherein, pre-treatment step includes that after cleaning turnip, the water that weight is 5-10 times of turnip, mashing processing is added；So
It is 5-15 minutes ultrasonic afterwards under the conditions of 20Hz-40Hz.
Wherein, pre-treatment step includes that the water that weight is 8 times of turnip is added, mashing processing；Then under the conditions of 20Hz,
Ultrasound 15 minutes.
According to another aspect of the present invention, extracting method protein obtained and/or polysaccharide are provided.
The present invention extracts protein using glusulase from turnip, and using ammonium sulfate and sodium sulphate mixing saturated solution into
Row is saltoutd, and is dialysed using three hydrogen aminomethane buffers.The glusulase is made from the prolixity capsule and alimentary canal of snail
Standby mixed enzyme, including fiber element enzyme, hemicellulase, pectase, Alpha amylase, seminase, invertase, galactan
A variety of biologically active mixed enzymes such as carbohydrase, proteolytic enzyme, aminotransferase, advantage outstanding is can be simultaneously
Cellulose, hemicellulose, glucan, the chitin etc. degraded in turnip cell wall, sporoderm-broken rate is very high, it can be made intracellular
Active material preferably releases, to further increase the yield of protein and polysaccharide in turnip.
It can be special in addition, being saltoutd in the present invention using the ammonium sulfate of different ratio and sodium sulphate mixing saturated solution
Property the protein for different molecular weight extract, when ammonium sulfate saturated solution with it is bigger when, the macromolecular egg of extraction
White matter content is more；When sodium sulphate saturated solution with it is bigger when, the small protein content of extraction is more.In addition, utilizing
The saturated solution of both different ratios can preferably extract turnip protein, while more optimize the extraction of rutabaga polysaccharide.
The invention has the following advantages:
1, the present invention is the method for extracting protein and polysaccharide simultaneously from turnip, and the average recoveries of protein are reachable
5.5% or more, the average recoveries of polysaccharide are up to 7.9% or more.
2, turnip protein proposed by the present invention can be added in varieties of food items directly as the nutrition fortifier of food,
Rutabaga polysaccharide can be directly as the raw material of health food and functional food.
Specific embodiment
The illustrative embodiments of the disclosure are described in more detail below.Although showing showing for the disclosure in specification
Example property embodiment, it being understood, however, that may be realized in various forms the disclosure without should be by embodiments set forth herein
It is limited.It is to be able to thoroughly understand the disclosure on the contrary, providing these embodiments, and can be by the model of the disclosure
It encloses and is fully disclosed to those skilled in the art.
Embodiment 1
The preparation method provided in this embodiment for extracting protein and polysaccharide simultaneously from turnip the following steps are included:
(1) pretreatment of turnip: after turnip being cleaned up with clear water, be added 5 times of turnip weight of water, using Mechanical Method
Carry out mashing processing；Then under the conditions of 20Hz, ultrasound 10 minutes.
(2) it digests: adding the glusulase for being 1:300 with the mass ratio of turnip in slurries, while the matter with turnip is added
For amount than the iodoacetic acid for being 1:1000, adjusting pH is 5.
(3) heat: the turnip slurries digested being poured into vessel in heating 30 minutes, temperature is set as 40 DEG C, adds
Hot complete cooling 12 hours.
(4) centrifugal treating: the turnip slurries after heat treatment are mixed into object and are put into high speed freezing centrifuge with 10000g's
Centrifugal force is centrifuged 20 minutes.
(5) plus neutral salt: the supernatant after taking out centrifugation, when environment temperature is 20 DEG C, adjusting pH is 5, be added with it is climing
Cyanines mass ratio is the ammonium sulfate saturated solution of 1:50 and the mixture of sodium sulphate saturated solution, and both mixtures ratio is 1:2, is stirred
It mixes uniformly, places 12 hours, be layered as supernatant liquor and lower sediment liquid.
(6) it extracts turnip protein: precipitated liquid is moved in bag filter, keep 2 DEG C of environment temperatures, and by the precipitated liquid
It dialyses 5 times in tri- hydrogen aminomethane buffer of 0.02mol/L, it is 20kDa that bag filter, which retains molecule, removes salt, cold
But it dries, obtains the protein finished product.
(7) it extracts rutabaga polysaccharide: supernatant being spray-dried, rutabaga polysaccharide is obtained.
Embodiment 2
The method provided in this embodiment for extracting protein and polysaccharide simultaneously from turnip the following steps are included:
(1) pretreatment of turnip: after turnip being cleaned up with clear water, be added 8 times of turnip weight of water, using Mechanical Method
Carry out mashing processing；Then under the conditions of 30Hz, ultrasound 15 minutes.
(2) it digests: adding the glusulase for being 1:500 with the mass ratio of turnip in slurries, while the matter with turnip is added
For amount than the iodoacetic acid for being 1:1000, adjusting pH is 7.
(3) heat: the turnip slurries digested being poured into vessel in heating 40 minutes, temperature is set as 50 DEG C, adds
Hot complete cooling 12 hours.
(4) centrifugal treating: the turnip slurries after heat treatment are mixed into object and are put into high speed freezing centrifuge with 10000g's
Centrifugal force is centrifuged 20 minutes.
(5) plus neutral salt: the supernatant after taking out centrifugation, when environment temperature is 30 DEG C, adjusting pH is 6, be added with it is climing
Cyanines mass ratio is the ammonium sulfate of 1:50 and the mixture of metabisulfite solution, and both mixtures ratio is 1:3, is stirred evenly,
It places 12 hours, is layered as supernatant liquor and lower sediment liquid.
(6) it extracts turnip protein: precipitated liquid is moved in bag filter, keep 2 DEG C of environment temperatures, and by the precipitated liquid
It dialyses 5 times in tri- hydrogen aminomethane buffer of 0.04mol/L, it is 30kDa that bag filter, which retains molecule, removes salt, cold
But it dries, obtains the protein finished product.
(7) it extracts rutabaga polysaccharide: supernatant being spray-dried, rutabaga polysaccharide is obtained.
Embodiment 3
The method provided in this embodiment for extracting protein and polysaccharide simultaneously from turnip the following steps are included:
(1) pretreatment of turnip: after turnip being cleaned up with clear water, be added 9 times of turnip weight of water, using Mechanical Method
Carry out mashing processing；Then under the conditions of 40Hz, ultrasound 15 minutes.
(2) it digests: adding the glusulase for being 1:500 with the mass ratio of turnip in slurries, while the matter with turnip is added
For amount than the iodoacetic acid for being 1:1000, adjusting pH is 7.
(3) heat: the turnip slurries digested being poured into vessel in heating 50 minutes, temperature is set as 60 DEG C, adds
Hot complete cooling 12 hours.
(4) centrifugal treating: the turnip slurries after heat treatment are mixed into object and are put into high speed freezing centrifuge with 10000g's
Centrifugal force is centrifuged 25 minutes.
(5) plus neutral salt: the supernatant after taking out centrifugation, when environment temperature is 30 DEG C, adjusting pH is 4, be added with it is climing
Cyanines mass ratio is the ammonium sulfate saturated solution of 1:50 and the mixture of sodium sulphate saturated solution, and both mixtures ratio is stirred for 1:1
It mixes uniformly, places 12 hours, be layered as supernatant liquor and lower sediment liquid.
(6) it extracts turnip protein: precipitated liquid is moved in bag filter, keep 2 DEG C of environment temperatures, and by the precipitated liquid
It dialyses 5 times in tri- hydrogen aminomethane buffer of 0.06mol/L, it is 30kDa that bag filter, which retains molecule, removes salt, cold
But it dries, obtains the protein finished product.
(7) it extracts rutabaga polysaccharide: supernatant being spray-dried, rutabaga polysaccharide is obtained.
Embodiment 4
The method provided in this embodiment for extracting protein and polysaccharide simultaneously from turnip the following steps are included:
(1) pretreatment of turnip: after turnip being cleaned up with clear water, be added 10 times of turnip weight of water, using machinery
Method carries out mashing processing；Then under the conditions of 40Hz, ultrasound 15 minutes.
(2) it digests: adding the glusulase for being 1:400 with the mass ratio of turnip in slurries, while the matter with turnip is added
For amount than the iodoacetic acid for being 1:1000, adjusting pH is 6.
(3) heat: the turnip slurries digested being poured into vessel in heating 40 minutes, temperature is set as 60 DEG C, adds
Hot complete cooling 12 hours.
(4) centrifugal treating: the turnip slurries after heat treatment are mixed into object and are put into high speed freezing centrifuge with 10000g's
Centrifugal force is centrifuged 30 minutes.
(5) plus neutral salt: the supernatant after taking out centrifugation, when environment temperature is 30 DEG C, adjusting pH is 7, be added with it is climing
Cyanines mass ratio is the ammonium sulfate saturated solution of 1:50 and the mixture of sodium sulphate saturated solution, and both mixtures ratio is 1:4, is stirred
It mixes uniformly, places 12 hours, be layered as supernatant liquor and lower sediment liquid.
(6) it extracts turnip protein: precipitated liquid is moved in bag filter, keep 2 DEG C of environment temperatures, and by the precipitated liquid
It dialyses 5 times in tri- hydrogen aminomethane buffer of 0.08mol/L, it is 40kDa that bag filter, which retains molecule, removes salt, cold
But it dries, obtains the protein finished product.
(7) it extracts rutabaga polysaccharide: supernatant being spray-dried, rutabaga polysaccharide is obtained.
Embodiment 5
The preparation method provided in this embodiment for extracting protein and polysaccharide simultaneously from turnip the following steps are included:
(1) pretreatment of turnip: after turnip being cleaned up with clear water, be added 5 times of turnip weight of water, using Mechanical Method
Carry out mashing processing；Then under the conditions of 20Hz, ultrasound 5 minutes.
(2) it digests: adding the glusulase for being 1:300 with the mass ratio of turnip in slurries, while the matter with turnip is added
For amount than the iodoacetic acid for being 1:800, adjusting pH is 7.
(3) heat: the turnip slurries digested being poured into vessel in heating 30 minutes, temperature is set as 70 DEG C, adds
Hot complete cooling 13 hours.
(4) centrifugal treating: the turnip slurries after heat treatment are mixed into object and are put into high speed freezing centrifuge with 10000g's
Centrifugal force is centrifuged 20 minutes.
(5) plus neutral salt: the supernatant after taking out centrifugation, when environment temperature is 20 DEG C, adjusting pH is 5, be added with it is climing
Cyanines mass ratio is the ammonium sulfate saturated solution of 1:45 and the mixture of sodium sulphate saturated solution, and both mixtures ratio is 1:1, is stirred
It mixes uniformly, places 20 hours, be layered as supernatant liquor and lower sediment liquid.
(6) it extracts turnip protein: precipitated liquid is moved in bag filter, keep 2 DEG C of environment temperatures, and by the precipitated liquid
It dialyses 4 times in tri- hydrogen aminomethane buffer of 0.02mol/L, it is 40kDa that bag filter, which retains molecule, removes salt, cold
But it dries, obtains the protein finished product.
(7) it extracts rutabaga polysaccharide: supernatant being spray-dried, rutabaga polysaccharide is obtained.
Embodiment 6
The preparation method provided in this embodiment for extracting protein and polysaccharide simultaneously from turnip the following steps are included:
(1) pretreatment of turnip: after turnip being cleaned up with clear water, be added 10 times of turnip weight of water, using machinery
Method carries out mashing processing；Then under the conditions of 40Hz, ultrasound 15 minutes.
(2) it digests: adding the glusulase for being 1:500 with the mass ratio of turnip in slurries, while the matter with turnip is added
For amount than the iodoacetic acid for being 1:1500, adjusting pH is 5.
(3) heat: the turnip slurries digested being poured into vessel in heating 50 minutes, temperature is set as 40 DEG C, adds
Hot complete cooling 8 hours.
(4) centrifugal treating: the turnip slurries after heat treatment are mixed into object and are put into high speed freezing centrifuge with 10000g's
Centrifugal force is centrifuged 30 minutes.
(5) plus neutral salt: the supernatant after taking out centrifugation, when environment temperature is 20 DEG C, adjusting pH is 5, be added with it is climing
Cyanines mass ratio is the ammonium sulfate saturated solution of 1:60 and the mixture of sodium sulphate saturated solution, and both mixtures ratio is 1:4, is stirred
It mixes uniformly, places 12 hours, be layered as supernatant liquor and lower sediment liquid.
(6) it extracts turnip protein: precipitated liquid is moved in bag filter, keep 2 DEG C of environment temperatures, and by the precipitated liquid
It dialyses 6 times in tri- hydrogen aminomethane buffer of 0.10mol/L, it is 10kDa that bag filter, which retains molecule, removes salt, cold
But it dries, obtains the protein finished product.
(7) it extracts rutabaga polysaccharide: supernatant being spray-dried, rutabaga polysaccharide is obtained.
Comparative example 1
The method provided in this embodiment for extracting protein and polysaccharide simultaneously from turnip the following steps are included:
(1) pretreatment of turnip: after turnip being cleaned up with clear water, be added 10 times of turnip weight of water, using machinery
Method carries out mashing processing；Then under the conditions of 40Hz, ultrasound 15 minutes.
(2) adjust pH: it is 7 that pH is adjusted after ultrasound.
(3) heat: turnip slurries being poured into vessel in heating 40 minutes, temperature is set as 60 DEG C, has heated cooling
12 hours.
(4) centrifugal treating: the turnip slurries after heat treatment are mixed into object and are put into high speed freezing centrifuge with 10000g's
Centrifugal force is centrifuged 30 minutes.
(5) plus neutral salt: the supernatant after taking out centrifugation, when environment temperature is 30 DEG C, adjusting pH is 6, be added with it is climing
Cyanines mass ratio is the ammonium sulfate saturated solution of 1:50 and the mixture of sodium sulphate saturated solution, and both mixtures ratio is stirred for 1:2
It mixes uniformly, places 12 hours, be layered as supernatant liquor and lower sediment liquid.
(6) it extracts turnip protein: precipitated liquid is moved in bag filter, keep 2 DEG C of environment temperatures, and by the precipitated liquid
It dialyses 5 times in tri- hydrogen aminomethane buffer of 0.08mol/L, it is 40kDa that bag filter, which retains molecule, removes salt, cold
But it dries, obtains the protein finished product.
(7) it extracts rutabaga polysaccharide: supernatant being spray-dried, rutabaga polysaccharide is obtained.
Comparative example 2
(1) pretreatment of turnip: after turnip being cleaned up with clear water, be added 10 times of turnip weight of water, using machinery
Method carries out mashing processing；Then under the conditions of 40Hz, ultrasound 15 minutes.
(2) it digests: adding the glusulase for being 1:400 with the mass ratio of turnip in slurries, while the matter with turnip is added
For amount than the iodoacetic acid for being 1:1000, adjusting pH is 6.
(3) it centrifugal treating: pH treated turnip slurries will be adjusted mixes object and be put into high speed freezing centrifuge with 10000g
Centrifugal force, be centrifuged 25 minutes.
(4) plus neutral salt: the supernatant after taking out centrifugation, when environment temperature is 30 DEG C, adjusting pH is 5, be added with it is climing
Cyanines mass ratio is the ammonium sulfate saturated solution of 1:50 and the mixture of sodium sulphate saturated solution, and both mixing ratio is 1:3, stirring
Uniformly, it places 12 hours, is layered as supernatant liquor and lower sediment liquid.
(5) it extracts turnip protein: precipitated liquid is moved in bag filter, keep 2 DEG C of environment temperatures, and by the precipitated liquid
It dialyses 5 times in tri- hydrogen aminomethane buffer of 0.06mol/L, it is 40kDa that bag filter, which retains molecule, removes salt, cold
But it dries, obtains the protein finished product.
(6) it extracts rutabaga polysaccharide: supernatant being spray-dried, rutabaga polysaccharide is obtained.
Comparative example 3
This extraction process selects cellulase as enzyme is extracted, and diisopropyl fluorophosphate (DFP) is as protease inhibitors, control
Other extraction conditions and parameter are consistent with embodiment 1.
Comparative example 4
This extraction process selects lysozyme as enzyme is extracted, other, which are extracted, uses object, extraction conditions and extracting parameter and reality
Example 1 is applied to be consistent.
The turnip place of production selected in above-described embodiment and comparative example is Hebei.Using ultraviolet light spectrophotometry to above-mentioned
Albumen and polysaccharide extract rate in embodiment and comparative example are measured, compare, analyze, and the results are shown in Table 1.
Table 1: the recovery rate of protein and polysaccharide in turnip
| Serial number | Experiment name | Protein average yield (%) | Polysaccharide average yield (%) |
| 1 | Embodiment 1 | 5.52% | 7.98% |
| 2 | Embodiment 2 | 5.93% | 8.21% |
| 3 | Embodiment 3 | 6.71% | 8.55% |
| 4 | Embodiment 4 | 6.07% | 8.41% |
| 5 | Embodiment 5 | 5.57% | 8.12% |
| 6 | Embodiment 6 | 5.84% | 7.91% |
| 7 | Comparative example 1 | 2.02% | 2.11% |
| 8 | Comparative example 2 | 1.82% | 3.05% |
| 9 | Comparative example 3 | 2.15% | 2.07% |
| 10 | Comparative example 4 | 2.21% | 2.15% |
As shown in Table 1, the content of the extracting method of 1-6 of the embodiment of the present invention is extracted turnip protein and polysaccharide is bright
It is aobvious to be higher than 1~comparative example of comparative example 4, and pass through the result content of the result content of embodiment 1 and comparative example 3 and comparative example 4
It was found that common bacterium wall breaking enzyme is little to the extraction positive effect of protein in turnip and polysaccharide, it is far below this hair
The effect of bright glusulase.
To sum up, the method for the present invention for extracting protein and polysaccharide simultaneously from turnip is made by the enzymatic hydrolysis of glusulase
With and control heating temperature, environment temperature, pH value, two kinds of salt different ratios, bag filter retain molecule, at turnip
Reason, to be conducive to the extraction separation of protein and polysaccharide in turnip.Further according to the change of protein generated under specific salinity
To change, protein isolate matter and polysaccharide component realize and disposably extract protein and polysaccharide from turnip, simplified production link,
The time is saved, the full fruit for improving turnip utilizes.In addition, turnip protein and polysaccharide that the method for the invention is extracted can be with
Raw material as health food and functional food.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by anyone skilled in the art,
It should be covered by the protection scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of the claim
Subject to enclosing.
Claims (10)
1. extracting the method for protein and polysaccharide from turnip, which comprises the following steps:
Slurries are made in turnip mashing, ultrasound by pretreatment；
Enzymatic hydrolysis is added glusulase and iodoacetic acid simultaneously to slurries, and adjusts pH to faintly acid；
Heating, faintly acid slurries are slowly heated, then are cooled to room temperature；
The cooling pulp centrifuged processing finished is obtained clear liquid by centrifugation；
It saltouts, again the pH of adjusting clear liquid to faintly acid, and under preference temperature, ammonium sulfate saturated solution is added and sodium sulphate is full
With the mixed solution of solution, stirs evenly and place layering；
The extraction of protein and polysaccharide, removal salt that the lower sediment liquid after layering is repeatedly dialysed, obtains turnip protein, will
Supernatant is dried to obtain rutabaga polysaccharide.
2. extracting method as described in claim 1, which is characterized in that
In enzymolysis step, the pH value of slurries is adjusted to 5~7, and the quality proportioning of glusulase and turnip is 1:300-500, iodoacetic acid
Quality proportioning with turnip is 1:800~1500.
3. extracting method as described in claim 1, which is characterized in that
In salting-out step, the ratio of ammonium sulfate saturated solution and sodium sulphate saturated solution is 1:1-4, mixed solution in mixed solution
Mass ratio with turnip is 1:45~60.
4. the extracting method as described in claims 1 or 2 or 3, which is characterized in that
In salting-out step, the pH value of supernatant is adjusted to 4~7, the environment temperature that the suitable temperature is 20~40 degree；It is described
Placing separation time is 12~20 hours.
5. extracting method as described in claim 1, which is characterized in that
The multiple dialysis is that lower sediment liquid is dialysed 4~6 times in three hydrogen aminomethane buffers.
6. extracting method as claimed in claim 5, which is characterized in that
Three hydrogen aminomethane buffer concentrations are 0.02mol/L-0.10mol/L, and the bag filter that the dialysis uses is cut
Staying molecule is 10-40kDa.
7. extracting method as described in claim 1, which is characterized in that
The heating temperature of heating stepses is 40~70 DEG C, and heating time is 30-50 minutes, and the cooling time is 8~13 hours.
8. extracting method as described in claim 1, which is characterized in that
Pre-treatment step includes, and after turnip is cleaned, the water that weight is 5-10 times of turnip, mashing processing is added；Then in 20Hz-
It is 5-15 minutes ultrasonic under the conditions of 40Hz.
9. extracting method as claimed in claim 8, which is characterized in that pre-treatment step includes that weight is added as 8 times of turnip
Water, mashing processing；Then under the conditions of 20Hz, ultrasound 15 minutes.
10. the extracting method protein obtained of protein and polysaccharide and/or more is extracted in turnip as described in claim 1-9
Sugar.
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| CN110964767A (en) * | 2019-12-30 | 2020-04-07 | 河北中科同创科技发展有限公司 | Method for extracting aster polysaccharide and aster polypeptide |
| CN112390897A (en) * | 2020-11-24 | 2021-02-23 | 邯郸学院 | Method for extracting and separating high-purity polysaccharide from plant flowers |
| CN114470026A (en) * | 2021-12-08 | 2022-05-13 | 湖南尔康制药股份有限公司 | Processing method of turnip paste |
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| CN112390897A (en) * | 2020-11-24 | 2021-02-23 | 邯郸学院 | Method for extracting and separating high-purity polysaccharide from plant flowers |
| CN114470026A (en) * | 2021-12-08 | 2022-05-13 | 湖南尔康制药股份有限公司 | Processing method of turnip paste |
| CN114470026B (en) * | 2021-12-08 | 2024-02-02 | 湖南尔康制药股份有限公司 | Processing method of turnip paste |
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Application publication date: 20190412 |