CN108329987A - A kind of method of microbe fermentation method extraction walnut health-care grease - Google Patents
A kind of method of microbe fermentation method extraction walnut health-care grease Download PDFInfo
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- CN108329987A CN108329987A CN201810176794.5A CN201810176794A CN108329987A CN 108329987 A CN108329987 A CN 108329987A CN 201810176794 A CN201810176794 A CN 201810176794A CN 108329987 A CN108329987 A CN 108329987A
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- walnut
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/008—Refining fats or fatty oils by filtration, e.g. including ultra filtration, dialysis
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
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Abstract
The invention discloses a kind of methods that microbe fermentation method extracts walnut health-care grease, this method is the ingredients such as protein and cellulose in the method decomposition walnut kernel by microbial fermentation, it is finally reached the purpose of Extraction and enrichment healthy oils, after walnut kernel plus water mashing, distiller's yeast of the addition rich in multiple-microorganism ferments, it is amino acid to make the breaks down proteins in walnut kernel, carbohydrate provides the energy source needed for microbial fermentation, finally obtain the grease zymotic fluid rich in walnut health-care grease, using the broken emulsification of cell crushing instrument, then walnut health-care grease crude oil is obtained by centrifugal filtration, then walnut health-care grease is obtained by refining.The present invention uses the protein in microbial degradation walnut kernel, the substances such as carbohydrate and cellulose to obtain the water-oil emulsion rich in walnut health-care grease, obtains being rich in amino acid after refining, flavor is special, does not remain the pure natural walnut health-care grease of any organic solvent.
Description
Technical field
The invention belongs to food processing fields, and in particular to a kind of side of microbe fermentation method extraction walnut health-care grease
Method.
Background technology
Walnut oil is to use walnut kernel for raw material, vegetable oil made of squeezing.Belong to edible oil.The fat content of walnut
Up to 65%-70%, shelter have first of woody oleiferous plants, there is the good reputation of " oil depot on tree ".It is carried and being selected the essence using modern crafts, this is just
It is walnut new generation product -- walnut oil.Walnut oil is to choose good walnut to do raw material, and use technique system leading in the world
The natural fruit oil juice taken out.In the international market, walnut oil is known as " east olive oil ", and consumption is equally received with olive oil
Person favors.According to analysis, fatty main component is glyceryl linoleate, leukotrienes and olein, these are all human body institutes
Required aliphatic acid.Walnut oil is that walnut kernel is made up of oil expression, refining, purification, and color and luster is yellow or brown color, is people
Ideal advanced edible cooking oil in daily life.
The preparation method of walnut oil mainly has carbon dioxide supercritical extraction, solution to leach extraction, water substitution, water phase at present
Enzymatic isolation method and squeezing method.Oil yield quality obtained by carbon dioxide supercritical extraction is good at present, but operating pressure is big, when extraction
Between it is long, and to equipment requirement height, investment is high, and oil product is expensive, and popularization is not easy.Solution leaches extraction, and there are intrinsic inflammable and explosive of solvent
Danger, while chemical solvent remain, influence oil product.Oil quality that water substitution and aqueous enzymatic extraction obtain is high, and crude oil without
It need to refine to obtain the final product, oil yield is high, but Cortex walnut seriously affects the taste and color of walnut oil without removing.Squeezing method is for I
The most common mode of state, oil yield is high, environmentally protective, but the Residual oil contained in the oil cake in squeezing method is high, and labor intensity is big,
It is of high cost.And cold-press, due to being carried out at a temperature of less than 60 DEG C, stability and oil product are all very high, but oil yield
It is low, and the process time is long, influences oil product popularization.
South China Science ＆ Engineering University Zhao scheme penetrating judgment, which is awarded, discloses an a kind of patent " method of high efficiency extraction walnut oil " patent No.：
The CN201510567265.4 patented methods are the substances such as protein and cellulose in degrading walnut kernel using biological enzyme formulation,
So that grease is fully discharged, it is strong that the fat globule interfacial film that nonionic surfactant makes walnut digest in emulsion is then added
Degree reduces, high to reach in conjunction with centrifugal separation technology further such that the fat globule in walnut enzymolysis emulsion ruptures
The purpose of effect extraction walnut oil.Make the degradations such as albumen and the cellulose in walnut kernel by mode of action, but this method is most
Enzyme deactivation is needed eventually, easily causes Oxidation of Fat and Oils, influences the flavor taste of walnut lubricant.
Invention content
It is an object of the invention to could grease be oxidizable rotten present in traditional enzymatic isolation method extraction walnut lubricant asks
Topic provides a kind of method of microbe fermentation method extraction walnut health-care grease, walnut kernel is decomposed by the method for microbial fermentation
In protein, the ingredients such as carbohydrate and cellulose, then Extraction and enrichment healthy oils are passed through using the broken emulsification of cell crushing instrument
It crosses centrifugal filtration and obtains walnut health-care grease crude oil, then obtain walnut health-care grease by refining.
The purpose of the present invention and its technical problem underlying is solved using following technical scheme to realize：A kind of microorganism
The method that fermentation method extracts walnut health-care grease, it is characterised in that include the following steps：
A. the pretreatment of walnut kernel：Walnut peeling is obtained into walnut kernel, by weight 1 portion of walnut kernel, 3-5 parts of pure water are added,
It is beaten using high speed beater, is uniformly mixed so as to obtain walnut kernel slurry；
B. walnut kernel adds koji fermentation：Walnut kernel slurry is heated to 25-30 DEG C, is fitted into ceramic wine jar, it is logical that efficient distiller's yeast is added
Aerobe fermentation, fermentation temperature are 25-30 DEG C, and the additive amount of efficient distiller's yeast is the 0.3-0.5% of walnut kernel slurry weight, fermentation time
It is 5-10 days, obtains the oil mixing with water zymotic fluid rich in walnut health-care grease after fermentation；
C. it is crushed emulsification：The oil mixing with water zymotic fluid that will be enriched in walnut health-care grease, is added in ultrasonic cell disruption instrument, stirs
It mixes 10-20 minutes broken, obtains oil mixing with water emulsion；
D. filter centrifugation:Oil mixing with water emulsion will be taken out in ultrasonic cell disruption instrument after gauze filtering to exist using centrifuge
Rotating speed is to centrifuge 5-15min under 5000-8000r/min, and supernatant liquor is taken to obtain walnut health-care grease crude oil；
E. crude oil refines:Obtained walnut health-care grease crude oil is passed through into depickling, is dewaxed, decoloration obtains after vacuum deodorization processing
Walnut health-care grease；
F. it examines filling：Walnut health-care grease by refining is filling after the assay was approved to obtain finished product grease.
The microbe species in efficient distiller's yeast wherein described in step B contain yeast, fungi, mould, and bacterium is therein
Two or more mixing；Ultrasonic cell disruption instrument operating condition is set as in step C：30-40 DEG C of temperature, ultrasonic power are
800-1000W。
The present invention has clear advantage and advantageous effect compared with prior art.From above technical scheme：
（1）The present invention sends out Extraction and enrichment walnut health-care grease using microbial fermentation, in extraction process temperature control 30 DEG C with
Under, it avoids Oxidation of Fat and Oils caused by high temperature and goes bad, influence flavor taste；
（2）Sonicator is used in the method for the present invention, is on the one hand the emulsification of walnut lubricant fat globule, grease extracts,
On the other hand by the bacteria inactivation rate in zymotic fluid, reduce high-temperature sterilization link, ensure that the quality of grease；
（3）It is acted on by microbial fermentation in the method for the present invention, is amino acid by protein degradation in walnut kernel, makes walnut lubricant
In be rich in amino acid, increase the health-care efficacy of walnut lubricant.
Figure of description
Fig. 1 is the process flow chart of the present invention.
Specific implementation mode
It below in conjunction with attached drawing and preferred embodiment, is described in further detail, but embodiments of the present invention are not limited to
This.
Embodiment 1
A kind of method of microbe fermentation method extraction walnut health-care grease, it is characterised in that include the following steps：
A. the pretreatment of walnut kernel：Walnut peeling is obtained into walnut kernel, by weight 1 portion of walnut kernel, 3 parts of pure water are added, adopt
It is beaten with high speed beater, is uniformly mixed so as to obtain walnut kernel slurry；
B. walnut kernel adds koji fermentation：Walnut kernel slurry is heated to 25 DEG C, is fitted into ceramic wine jar, efficient distiller's yeast is added and leads to oxygen hair
Ferment, fermentation temperature are 25 DEG C, and the additive amount of efficient distiller's yeast is the 0.3% of walnut kernel slurry weight, and fermentation time is 5 days, fermentation knot
The oil mixing with water zymotic fluid rich in walnut health-care grease is obtained after beam；
C. it is crushed emulsification：The oil mixing with water zymotic fluid that will be enriched in walnut health-care grease, is added in ultrasonic cell disruption instrument, surpasses
Sound wave cell crushing instrument operating condition is set as：30 DEG C of temperature, ultrasonic power 800W；Stirring is 10 minutes broken, obtains grease
Mix emulsion fluid；
D. filter centrifugation:Oil mixing with water emulsion will be taken out in ultrasonic cell disruption instrument, after gauze filtering, using centrifuge
55min is centrifuged in the case where rotating speed is 5000r/min, supernatant liquor is taken to obtain walnut health-care grease crude oil；
E. crude oil refines:Obtained walnut health-care grease crude oil is passed through into depickling, is dewaxed, decoloration obtains after vacuum deodorization processing
Walnut health-care grease；
F. it examines filling：Walnut health-care grease by refining is filling after the assay was approved to obtain finished product grease.
Embodiment 2
A kind of method of microbe fermentation method extraction walnut health-care grease, it is characterised in that include the following steps：
A. the pretreatment of walnut kernel：Walnut peeling is obtained into walnut kernel, by weight 1 portion of walnut kernel, 4 parts of pure water are added, adopt
It is beaten with high speed beater, is uniformly mixed so as to obtain walnut kernel slurry；
B. walnut kernel adds koji fermentation：Walnut kernel slurry is heated to 28 DEG C, is fitted into ceramic wine jar, efficient distiller's yeast is added and leads to oxygen hair
Ferment, fermentation temperature are 28 DEG C, and the additive amount of efficient distiller's yeast is the 0.4% of walnut kernel slurry weight, and fermentation time is 8 days, fermentation knot
The oil mixing with water zymotic fluid rich in walnut health-care grease is obtained after beam；
C. it is crushed emulsification：The oil mixing with water zymotic fluid that will be enriched in walnut health-care grease, is added in ultrasonic cell disruption instrument, surpasses
Sound wave cell crushing instrument operating condition is set as：35 DEG C of temperature, ultrasonic power 900W；Stirring is 15 minutes broken, obtains grease
Mix emulsion fluid；
D. filter centrifugation:Oil mixing with water emulsion will be taken out in ultrasonic cell disruption instrument after gauze filtering to exist using centrifuge
Rotating speed is to centrifuge 10min under 6000r/min, and supernatant liquor is taken to obtain walnut health-care grease crude oil；
E. crude oil refines:Obtained walnut health-care grease crude oil is passed through into depickling, is dewaxed, decoloration obtains after vacuum deodorization processing
Walnut health-care grease；
F. it examines filling：Walnut health-care grease by refining is filling after the assay was approved to obtain finished product grease.
Embodiment 3
A kind of method of microbe fermentation method extraction walnut health-care grease, it is characterised in that include the following steps：
A. the pretreatment of walnut kernel：Walnut peeling is obtained into walnut kernel, by weight 1 portion of walnut kernel, 5 parts of pure water are added, adopt
It is beaten with high speed beater, is uniformly mixed so as to obtain walnut kernel slurry；
B. walnut kernel adds koji fermentation：Walnut kernel slurry is heated to 30 DEG C, is fitted into ceramic wine jar, efficient distiller's yeast is added and leads to oxygen hair
Ferment, fermentation temperature are 30 DEG C, and the additive amount of efficient distiller's yeast is the 0.5% of walnut kernel slurry weight, and fermentation time is 10 days, fermentation
After obtain the oil mixing with water zymotic fluid rich in walnut health-care grease；
C. it is crushed emulsification：The oil mixing with water zymotic fluid that will be enriched in walnut health-care grease, is added in ultrasonic cell disruption instrument, surpasses
Sound wave cell crushing instrument operating condition is set as：30 DEG C of temperature, ultrasonic power 1000W；Stirring is 20 minutes broken, obtains grease
Mix emulsion fluid；
D. filter centrifugation:Oil mixing with water emulsion will be taken out in ultrasonic cell disruption instrument after gauze filtering to exist using centrifuge
Rotating speed is to centrifuge 15min under 8000r/min, and supernatant liquor is taken to obtain walnut health-care grease crude oil；
E. crude oil refines:Obtained walnut health-care grease crude oil is passed through into depickling, is dewaxed, decoloration obtains after vacuum deodorization processing
Walnut health-care grease；
F. it examines filling：Walnut health-care grease by refining is filling after the assay was approved to obtain finished product grease.
The above described is only a preferred embodiment of the present invention, not being made any form of restriction to inventing type, appoint
What is simply repaiied to any made by above example according to the technical essence of the invention without departing from technical solution of the present invention content
Change, equivalent variations and modification, in the range of still falling within technical solution of the present invention.
Claims (3)
1. a kind of method of microbe fermentation method extraction walnut health-care grease, it is characterised in that include the following steps：
A. the pretreatment of walnut kernel：Walnut peeling is obtained into walnut kernel, by weight 1 portion of walnut kernel, 3-5 parts of pure water are added,
It is beaten using high speed beater, is uniformly mixed so as to obtain walnut kernel slurry；
B. walnut kernel adds koji fermentation：Walnut kernel slurry is heated to 25-30 DEG C, is fitted into ceramic wine jar, it is logical that efficient distiller's yeast is added
Aerobe fermentation, fermentation temperature are 25-30 DEG C, and the additive amount of efficient distiller's yeast is the 0.3-0.5% of walnut kernel slurry weight, fermentation time
It is 5-10 days, obtains the oil mixing with water zymotic fluid rich in walnut health-care grease after fermentation；
C. it is crushed emulsification：The oil mixing with water zymotic fluid that will be enriched in walnut health-care grease, is added in ultrasonic cell disruption instrument, stirs
It mixes 10-20 minutes broken, obtains oil mixing with water emulsion；
D. filter centrifugation:Oil mixing with water emulsion will be taken out in ultrasonic cell disruption instrument after gauze filtering to exist using centrifuge
Rotating speed is to centrifuge 5-15min under 5000-8000r/min, and supernatant liquor is taken to obtain walnut health-care grease crude oil；
E. crude oil refines:Obtained walnut health-care grease crude oil is passed through into depickling, is dewaxed, decoloration obtains after vacuum deodorization processing
Walnut health-care grease；
F. it examines filling：Walnut health-care grease by refining is filling after the assay was approved to obtain finished product grease.
2. a kind of method of microbe fermentation method extraction walnut health-care grease according to claim 1, which is characterized in that step
The microbe species in efficient distiller's yeast described in rapid B contain yeast, fungi, mould, bacterium two or more mixing therein.
3. a kind of method of microbe fermentation method extraction walnut health-care grease according to claim 1, which is characterized in that step
Ultrasonic cell disruption instrument operating condition is set as in rapid C：30-40 DEG C of temperature, ultrasonic power 800-1000W.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810176794.5A CN108329987A (en) | 2018-03-04 | 2018-03-04 | A kind of method of microbe fermentation method extraction walnut health-care grease |
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| CN201810176794.5A CN108329987A (en) | 2018-03-04 | 2018-03-04 | A kind of method of microbe fermentation method extraction walnut health-care grease |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112641660A (en) * | 2021-01-12 | 2021-04-13 | 江苏瑞霆生物科技有限公司 | Preparation method of high-stability vegetable oil material fermentation self-packaging carrier, product and application thereof |
| CN115261110A (en) * | 2022-06-30 | 2022-11-01 | 金华市农业科学研究院(浙江省农业机械研究院) | Process for extracting oil from tea seed cake by water fermentation method |
-
2018
- 2018-03-04 CN CN201810176794.5A patent/CN108329987A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112641660A (en) * | 2021-01-12 | 2021-04-13 | 江苏瑞霆生物科技有限公司 | Preparation method of high-stability vegetable oil material fermentation self-packaging carrier, product and application thereof |
| CN112641660B (en) * | 2021-01-12 | 2021-10-22 | 江苏瑞霆生物科技有限公司 | Preparation method of high-stability vegetable oil material fermentation self-packaging carrier, product and application thereof |
| CN115261110A (en) * | 2022-06-30 | 2022-11-01 | 金华市农业科学研究院(浙江省农业机械研究院) | Process for extracting oil from tea seed cake by water fermentation method |
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