CN112641660A

CN112641660A - Preparation method of high-stability vegetable oil material fermentation self-packaging carrier, product and application thereof

Info

Publication number: CN112641660A
Application number: CN202110038184.0A
Authority: CN
Inventors: 邓楠; 赵炳天; 杜养标
Original assignee: Jiangsu Ruiting Biotechnology Co ltd
Current assignee: Jiangsu Ruiting Biotechnology Co ltd
Priority date: 2021-01-12
Filing date: 2021-01-12
Publication date: 2021-04-13
Anticipated expiration: 2041-01-12
Also published as: CN112641660B

Abstract

The invention discloses a preparation method of a high-stability plant oil material fermentation self-packaging carrier, a product and application thereof. The carrier system has high stability, and can not be demulsified at centrifugation, high temperature and low temperature. The fermented product is rich in protein, amino acids, polyphenol, and polysaccharide. The fermented product is green and safe, has no hemolytic effect, no adverse reaction on human skin, and no irritation. The invention is a novel development and utilization of the vegetable oil, and is beneficial to improving the economic application value of the vegetable oil in cosmetics.

Description

Preparation method of high-stability vegetable oil material fermentation self-packaging carrier, product and application thereof

Technical Field

The invention belongs to the technical field of skin care products, and particularly relates to a preparation method of a high-stability vegetable oil material fermentation self-packaging carrier, a product and application thereof.

Background

The oil can form a hydrophobic film on the surface of the skin, so that the skin is soft, smooth and glossy; under dry and cold climate conditions, the evaporation of moisture on the surface of the skin is inhibited, and the skin is prevented from being dried and cracked; as a solvent for the specific ingredients, promoting the absorption of the drug or active ingredient into the skin; imparting a soft and shiny feel to the hair.

The vegetable oil is close to the structure of human skin lipid membrane due to the texture, is easier to be absorbed by the skin, has better permeability, and is widely applied to the field of skin care products. Examples of vegetable oils commonly used in cosmetics in recent years include olive oil, jojoba oil, camellia seed oil, almond oil, and the like. At present, most vegetable oil is extracted by chemical and physical processes, and other abundant proteins, water-soluble vitamins and the like contained in vegetable oil are discarded; the traditional biological fermentation method is mostly carried out in water phase, and the traditional fermentation process is not compatible with vegetable oil.

Therefore, there is a need in the art for a method of producing fermented vegetable oil that efficiently converts proteins, polysaccharides, etc. into small-molecule water-soluble fermentation systems that are easily absorbed by the skin, and at the same time entraps vegetable oil, maximizing the nutrients of the vegetable oil.

Disclosure of Invention

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made keeping in mind the above and/or other problems occurring in the prior art.

Therefore, the invention aims to overcome the defects in the prior art and provide a preparation method of a high-stability vegetable oil material fermentation self-packaging carrier.

In order to solve the technical problems, the invention provides the following technical scheme: a preparation method of a high-stability vegetable oil material fermentation self-wrapping carrier comprises the steps of inoculating microorganisms into a fermentation substrate consisting of vegetable oil materials and glucose, performing fermentation culture, breaking cells and releasing cell membranes to obtain the high-stability vegetable oil material fermentation self-wrapping carrier; wherein, in the fermentation process, the cell membrane of the microorganism is used as a surfactant to encapsulate fat.

As a preferable scheme of the preparation method of the high-stability vegetable oil material fermentation self-packaging carrier, the method comprises the following steps: the vegetable oil material comprises one or more of camellia seed, almond, coconut, jojoba, white tea, olive, peanut, walnut, sunflower seed, castor and sesame.

As a preferable scheme of the preparation method of the high-stability vegetable oil material fermentation self-packaging carrier, the method comprises the following steps: the microorganism comprises one or more of yeast, lactobacillus, bacillus and aspergillus.

As a preferred scheme of the preparation method of the high-stability vegetable oil material fermentation self-packaging carrier, the method comprises the following steps: comprises pulverizing vegetable oil, sterilizing, and mixing with sterile glucose to obtain mixed solution; adding microbial bacteria liquid into the sterilized slurry according to the volume ratio of 5-8%, and fermenting for 36-48 h at the temperature of 25-30 ℃; the ventilation rate is 3-5 sL.min when the fermentation process is carried out in a fermentation tank^-1The pH value is 5-5.5; filtering the fermented liquid by adopting 250-300-mesh silk cloth, removing macromolecular particles while retaining microbial cells, and collecting filtrate; homogenizing the filtrate at high pressure of 1000-1500 bar for 5-6 times to break cells, release cell membranes as surfactant and entrap vegetable oil; and filtering the homogenized filtrate by using a ceramic membrane, and collecting the filtrate, namely the vegetable oil material fermentation self-packaging carrier.

It is a further object of the present invention to overcome the deficiencies of the prior art and to provide a product prepared by the method for preparing a high stability vegetable oil fermentation self-packaging carrier.

In order to solve the technical problems, the invention provides the following technical scheme: the product is prepared by a preparation method of the high-stability vegetable oil material fermentation self-wrapping carrier, no additional surfactant is needed, and the microbial cell membrane carries fat with the particle size of 130-180 nm, and stably exists in fermentation liquor.

As a preferable scheme of the product prepared by the preparation method of the high-stability vegetable oil material fermentation self-packaging carrier, the method comprises the following steps: the fermented self-coated carrier product is still stably coated with the grease after centrifugation.

As a preferable scheme of the product prepared by the preparation method of the high-stability vegetable oil material fermentation self-packaging carrier, the method comprises the following steps: the fermented self-coated carrier product is still stably coated with the grease after being placed at a high temperature of 50 ℃ for one month.

As a preferable scheme of the product prepared by the preparation method of the high-stability vegetable oil material fermentation self-packaging carrier, the method comprises the following steps: the fermented self-coated carrier product is still stably coated with the grease after being placed for one month at 4 ℃ and 20 ℃ in sequence.

As a preferable scheme of the product prepared by the preparation method of the high-stability vegetable oil material fermentation self-packaging carrier, the method comprises the following steps: the fermentation self-packaging carrier product is rich in protein, amino acid, polyphenol and polysaccharide.

Another object of the present invention is to overcome the disadvantages of the prior art, and to provide a method for preparing a high-stability vegetable oil fermentation self-contained carrier, which can be used in cosmetics, wherein: the high-stability vegetable oil material fermentation self-packaging carrier is green and safe, has no hemolytic effect, causes no adverse reaction to skin, and has no irritation.

The invention has the beneficial effects that:

(1) the invention discloses a preparation method of a high-stability vegetable oil material fermentation self-packaging carrier.

(2) The prepared coating carrier system contains rich protein, amino acid, polyphenol and polysaccharide, has high stability, can not be demulsified at centrifugation, high temperature and low temperature, is green and safe when being applied to cosmetics, has no hemolytic effect, has no adverse reaction on human skin, and has no irritation.

(3) The invention is a novel development and utilization of the vegetable oil, and is beneficial to improving the economic application value of the vegetable oil in cosmetics.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise. Wherein:

FIG. 1 is a graph showing the results of comparison of fat forms of samples in examples of the present invention.

FIG. 2 is a graph showing the particle size of the fermented self-contained carrier of vegetable oil plants in example 1 of the present invention.

Fig. 3 is a grease entrapment effect diagram of the sample in example 1 of the present invention, and the grease entrapment effect diagrams prepared by samples 1 to 6 are shown from left to right in sequence.

Fig. 4 is a grease entrapment effect diagram of a sample in example 7 of the present invention, and the grease entrapment effect diagrams obtained in tests 1 to 4 are shown sequentially from left to right.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, specific embodiments thereof are described in detail below with reference to examples of the specification.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.

The vegetable oil material of the present invention includes, but is not limited to, camellia seed, almond, coconut, jojoba, white tea, olive, peanut, walnut, sunflower seed, castor, sesame. The present invention is described in detail by taking camellia seeds as an example.

The microorganism of the invention includes but is not limited to yeast, lactic acid bacteria, bacillus and aspergillus. The present invention will be described in detail by taking Saccharomyces cerevisiae as an example.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

The camellia seeds in the following examples are commercially available.

Saccharomyces cerevisiae CICC 1210 used in the examples was purchased from China center for Industrial culture Collection of microorganisms.

The YPD liquid culture medium comprises the following components in percentage by weight: 10.0g of yeast powder, 20.0g of peptone, 20.0g of glucose, 6.5-6.7 of pH and 1000mL of deionized water.

The YPD solid culture medium is YPD liquid culture medium added with 2% agar powder.

The culture medium components are prepared and sterilized at 121 deg.C for 20 min.

Example 1

Camellia seed fermentation self-entrapping carrier

1. Seed liquid preparation

Under aseptic conditions, a single colony of Saccharomyces cerevisiae was picked and inoculated into YPD liquid medium. 30 ℃ and 200r min^-1Shaking and culturing for 24h until OD600 is about 16 to obtain seed liquid.

2. Stock preparation

Cleaning camellia seeds with deionized water, and draining; adding into a wall breaking machine, adding deionized water with 3 times of mass ratio, and crushing until no large particle solid is visible.

3. Preparation of fermentation substrate

Camellia seed 40 g.L^-1Glucose, glucose20g·L^-1. Sterilizing at 121 deg.C for 20min to obtain fermentation substrate, and cooling.

4. Camellia seed fermentation

Adding saccharomyces cerevisiae seed liquid with the volume ratio of 5% into the sterilized slurry, and fermenting for 48h at the temperature of 30 ℃; the ventilation rate is 4 sL.min when the fermentation process is carried out in a fermentation tank^-1The pH was 5.5.

5. Filtration

Filtering the fermentation liquor by using 250-mesh silk cloth, removing macromolecular particles while retaining microbial cells, and collecting filtrate.

6. Disrupting cells

The high-pressure homogenizing pressure is 1000bar, the high-pressure homogenizing is carried out for 1-6 times, cells are broken, cell membranes are released to serve as surfactants, vegetable oil is entrapped, and the entrapment effect of the oil is shown in table 1 and figure 3. For the purpose of photographing clearly, oil red is used for dyeing the grease of the samples 1-4.

TABLE 1 influence of high-pressure homogenization times on the oil entrapment Effect

When the high-pressure homogenization frequency is insufficient, the cells are not completely crushed, the cell membranes are not completely released, the oil entrapment effect is poor, and the layering phenomenon occurs. And when the high-pressure homogenization is carried out for 5-6 times, the oil entrapment effect is good. The number of high-pressure homogenization was determined to be 5 times in consideration of economic factors.

7. Microfiltration

Filtering by using a ceramic membrane with the diameter of 1 mu m, and collecting filtrate to obtain the high-stability vegetable oil material fermentation self-wrapping carrier.

Example 2

The difference from example 1 is that: no high pressure homogenization was performed and no cell membranes were released.

Example 3

Contrast of microscopic oil droplet state

1. Preparation of the test specimens

Example 1 (the number of high-pressure homogenization times is 5) and example 2 of the present invention were used as samples to be tested.

2. Nile Red dyeing

Nile red is a fat-soluble dye which can dye the lipid in the fermentation liquor into red. Nile red does not fluoresce in most polar solvents, however, in lipid rich environments, it can fluoresce strongly.

Nile red dye liquor storage liquid: 10mg of nile red and 10mL of acetone, fully blending and storing in dark.

Nile red working solution: nile Red stock solution 100. mu.L, PBS (pH 7.4)1mL, fully mixed and used.

Taking 1mL of sample to be detected, adding 1mL of nile red working solution under the condition of keeping out of the sun, and incubating for 5 min. 10 μ L of the slide glass was covered with a cover glass, and the fluorescence of the prepared slide was observed by scanning with a confocal laser microscope. The oil droplet state was observed.

3. Oil droplet state contrast

The fat morphology was observed under a fluorescence microscope, as shown in FIG. 1, wherein (a) example 1 and (b) example 2. The grease of example 1 was round, whereas the grease of example 2 was irregular in shape, in a radial pattern. Compared with example 2, the oil droplets in example 1 (the number of times of high-pressure homogenization is 5) are more uniformly distributed, which shows that cell membrane fragments released in the cell lysis process can assemble by themselves to form liposomes, and can form a monolayer membrane to wrap the oil droplets.

Example 4

Particle size comparison

1. Preparation of the test specimens

2. Particle size detection

And (3) measuring the particle size of the sample by using a particle size analyzer, and directly measuring the measured sample in a sample cell.

The particle size of the sample was measured by laser light scattering. The sample is diluted by deionized water and then put into a Zeta PALS type potential and nanometer particle size analyzer, the scattering angle is set to be 90 degrees, and the parallel determination is carried out for 3 times.

3. Comparison of particle size

As shown in FIG. 2, the particle size of example 1 was 146.1nm, and the oil in camellia seeds was entrapped by the cell membrane. While the particle size of example 2 was too large to be measured by the instrument.

Example 5

Stability of camellia seed fermentation self-packaging carrier system

1. Centrifugal stability

(1) Preparation of the test specimens

The sample to be tested in example 1 (camellia seed self-entrapping fermentation broth) of the present invention was used.

(2) Centrifugation

The sample is put at 3000 r.min^-1And centrifuging for 40min under the condition, and observing whether oil drops are separated out or not.

(3) Results

The camellia seeds are separated out without oil drops on the surface after being centrifuged from the entrapment fermentation liquor, which shows that the stability of an entrapment system is higher.

2. Temperature stability

(1) Preparation of the test specimens

The sample to be tested in example 1 (camellia seed self-entrapped fermentation liquid, high-pressure homogenization times of 5 times) of the invention was used.

(2) Experimental methods

The sample to be measured was placed at high temperature (50 ℃) and low temperature (4 ℃ to-20 ℃) for 1 month, and whether oil droplets were precipitated or not was observed to determine the particle size.

(3) Results

No oil drop is separated out when the sample is placed for 1 month in the high temperature group (50 ℃) and the low temperature group (4 ℃, -20 ℃). The particle size of the 50 ℃ sample was 186.4nm, the particle size of the 4 ℃ sample was 161.2nm, and the particle size of the-20 ℃ sample was 160.5 nm. Indicating that the temperature stability of the entrapped system is higher.

Example 6

Determination of active content

1. Preparation of the test specimens

2. Active matter content determination method

Total protein content in the samples was determined with reference to Lowry Method: bovine serum albumin solutions of different concentrations were prepared to draw a standard curve. 0.5mL of bovine serum albumin solution was added to 5mL of basic copper solution. After standing at room temperature for 20min, 0.5mL of 50% by mass Folin's phenol reagent was added to the above solution. Standing at room temperature for 30min, selecting 650nm wavelength, and measuring absorbance. And drawing a standard curve by taking the absorbance as a vertical coordinate and the concentration of the bovine serum albumin solution as a horizontal coordinate. And (4) measuring the absorbance of the sample to be measured according to the method, and substituting the absorbance into the standard curve to calculate the protein content.

And (3) determining the content of the total amino acids in the sample by referring to a ninhydrin color development method: glutamic acid solutions of different concentrations were prepared to draw a standard curve. 2mL of glutamic acid solution, 0.5mL of PBS buffer, and 0.5mL of 2% ninhydrin solution were added to the tube, and the tube was heated in a boiling water bath for 20 min. The absorbance was measured at a wavelength of 570 nm. And drawing a standard curve by taking the absorbance as an ordinate and the concentration of the glutamic acid solution as an abscissa. And (4) measuring the absorbance of the sample to be measured according to the method, and substituting the absorbance into the standard curve to calculate the total amino acid content.

The method for measuring the total phenol content of the sample comprises the following steps: gallic acid solutions of different concentrations were prepared to draw standard curves. Adding 100 μ L gallic acid solution, 1.0mL forskolin phenol, 1.0mL 10% Na₂CO₃And reacting at normal temperature for 90 min. The absorbance was measured at a wavelength of 760 nm. And drawing a standard curve by taking the absorbance as an ordinate and the concentration of the gallic acid solution as an abscissa. And (4) measuring the absorbance of the sample to be measured according to the method, and substituting the absorbance into the standard curve to calculate the total phenol content.

The method for measuring the total sugar content of the sample comprises the following steps: glucose solutions of different concentrations were prepared to draw a standard curve. 1.0mL of a 5% aqueous solution of phenol, 1.0mL of a glucose solution, and 5.0mL of concentrated sulfuric acid were added to a test tube, shaken, and reacted in boiling water for 25 min. The absorbance was measured at 490 nm. The absorbance was plotted as the ordinate and the concentration of the glucose solution as the abscissa to draw a standard curve. And (4) measuring the absorbance of the sample to be measured according to the method, and substituting the absorbance into the standard curve to calculate the total sugar content.

The active matter content of the camellia seed self-entrapping fermentation liquor is shown in table 2. The self-loading fermentation liquor of camellia seeds contains rich protein, amino acid, phenol and sugar.

TABLE 2 Camellia seed active content of self-entrapped fermentation broth

Example 7

The effect of different fermentation parameters on the performance of the vegetable oil fermented self-coated carrier was investigated under the conditions of example 1 (5 times of high pressure homogenization), and the conditions and results are shown in table 3 and fig. 4:

test 1: the volume ratio of the saccharomyces cerevisiae seed liquid is 2 percent;

test 2: the volume ratio of the saccharomyces cerevisiae seed liquid is 7 percent;

test 3: fermenting for 24h at 30 ℃;

test 4: fermenting at 30 deg.C for 60 h.

TABLE 3

It can be seen that the inoculum size of the saccharomyces cerevisiae was reduced in test 1 compared to example 1, the fermentation time was insufficient in test 3, and the oil and water phases immediately separated after high pressure homogenization. The inoculation amount of fermentation is too low or the fermentation time is insufficient, the bacterial quantity is insufficient at the end of fermentation, and sufficient cell membranes cannot be released to entrap grease. In comparison with example 1, the inoculum size of the Saccharomyces cerevisiae was increased in test 2, the fermentation time was increased in test 4, and the samples were allowed to stand at 50 ℃ for a period of time and then stratified. Too much inoculation amount or too long fermentation time of fermentation, too high contents of protein, polyphenol and the like in a fermentation system and the stability of the system can be reduced. The carrier system of the invention contains abundant protein, amino acid, polyphenol and polysaccharide, and has high stability, while different fermentation parameters influence the content of protein and polyphenol in the final system, and the content of protein, polyphenol and the like also influences the stability of the system finally.

Example 8

Evaluation of safety

1. Haemolysis test of erythrocytes

(1) Experimental Material

Pig red blood cells (2%, Beijing Borxi technology Co., Ltd.);

PBS: 0.27g (2mM) KH2PO4, 3.58g (10mM) Na2HPO4, 8g NaCl and 0.2g KCl were dissolved in 1L deionized water, and the pH was adjusted to 7.4 with hydrochloric acid.

(2) Experimental methods

Control group of total hemolyzed blood: water + porcine red blood cells

Negative control group: PBS + porcine red blood cell

Sample group: example 1 (Camellia seed self-entrapped fermentation broth) + pig red blood cells

Hemolysis test: dispersing the sample in PBS or physiological saline, mixing erythrocyte and sample at a ratio of 2:3, incubating at 37 deg.C for 10min, centrifuging at 10000 r.min-1 for 3min, and collecting supernatant to test absorbance at 540 nm. The hemolysis rate is calculated as: HD% (A540-like-A540 PBS)/(A540 Water-A540 PBS) × 100%

HD50 was calculated by setting 5 points above and below HD50, and setting a standard curve with the sample concentration as the abscissa and HD as the ordinate.

(3) HD50 of camellia seed self-entrapped fermentation liquid

The camellia seeds have no hemolytic effect under the maximum experimental concentration (90 percent, and a certain amount of PBS needs to be added into the liquid to remove hemolysis caused by the difference of intracellular and extracellular osmotic pressures), so the HD50 is more than 90 percent.

2. Human body skin spot test (closed type)

(1) Experimental methods

According to the method specified in 2015 cosmetic specifications.

The volunteer requires: 30 (20-40 years old) normal subjects, patients who do not have clinical unhealed inflammatory dermatosis, judges that the skin to-be-tested part has no scar, pigment, atrophy, port nevus flammeus or other flaws, does not participate in other clinical test researchers, patients who have no high-sensitivity physique, and patients who have not been subjected to a spot test in the last month.

Sample group: example 1 (Camellia seed self-entrapping fermentation broth)

Negative control group: deionized water

The solutions are added on a filter paper sheet attached to a spot tester and placed in the spot tester, the dosage is about 0.020-0.025 mL, the spot tester added with the tested object is pasted on the curved side of the forearm by using a non-irritating adhesive tape, and the patch is lightly pressed by hands and hands to be uniformly pasted on the skin for 24 hours. And (5) removing the tested substance spot tester for 30min, and observing skin reaction after the indentation disappears. If the result is negative, the reaction result is recorded by observing 24h and 48h after the patch test respectively.

(2) Results

TABLE 3 human body skin Patch test results (closed type)

As shown in Table 3, 30 human body patch experimental subjects showed 0-grade reaction, and according to the regulation of 2015 cosmetic hygiene specifications, the camellia seed self-entrapped fermentation broth did not cause adverse reaction to human skin, so that the product has high human body safety.

At present, most of the vegetable oil is encapsulated with an exogenously added surfactant, and cell membrane phospholipid released after microbial thalli are cracked is the most abundant and natural biological surfactant. Most of the existing fermentation systems are water phases, and oil of vegetable oil cannot exist stably in the fermentation systems; at present, the grease can only exist in a water phase by adding a surfactant from an external source for entrapment. The invention can entrap grease through the cell membrane of the microorganism in the fermentation process without adding other components additionally.

The invention discloses a preparation method of a high-stability vegetable oil material fermentation self-packaging carrier. The prepared coating carrier system contains rich protein, amino acid, polyphenol and polysaccharide, has high stability, can not be demulsified at centrifugation, high temperature and low temperature, is green and safe when being applied to cosmetics, has no hemolytic effect, has no adverse reaction on human skin, and has no irritation. The invention is a novel development and utilization of the vegetable oil, and is beneficial to improving the economic application value of the vegetable oil in cosmetics.

It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.

Claims

1. A preparation method of a high-stability vegetable oil material fermentation self-packaging carrier is characterized by comprising the following steps: comprises the steps of (a) preparing a mixture of a plurality of raw materials,

inoculating microorganisms into a fermentation substrate consisting of vegetable oil and glucose, fermenting and culturing, and breaking cells to release cell membranes to obtain the high-stability vegetable oil fermentation self-packaging carrier; wherein the content of the first and second substances,

during the fermentation process, the cell membrane of the microorganism is used as a surfactant to encapsulate fat.

2. The method for preparing a high-stability vegetable oil fermentation self-contained carrier as claimed in claim 1, wherein: the vegetable oil material comprises one or more of camellia seed, almond, coconut, jojoba, white tea, olive, peanut, walnut, sunflower seed, castor and sesame.

3. The method for preparing a high-stability vegetable oil fermentation self-contained carrier as claimed in claim 1, wherein: the microorganism comprises one or more of yeast, lactobacillus, bacillus and aspergillus.

4. The method for preparing the high-stability vegetable oil material fermentation self-packaging carrier as claimed in any one of claims 1 to 3, wherein the method comprises the following steps: comprises the steps of (a) preparing a mixture of a plurality of raw materials,

crushing the vegetable oil, sterilizing, and mixing with sterile glucose to obtain a mixed solution;

adding microbial bacteria liquid into the sterilized slurry according to the volume ratio of 5-8%, and fermenting for 36-48 h at the temperature of 25-30 ℃; the ventilation rate is 3-5 sL.min when the fermentation process is carried out in a fermentation tank^-1The pH value is 5-5.5;

filtering the fermented liquid by adopting 250-300-mesh silk cloth, removing macromolecular particles while retaining microbial cells, and collecting filtrate;

homogenizing the filtrate at high pressure of 1000-1500 bar for 5-6 times to break cells, release cell membranes as surfactant and entrap vegetable oil;

and filtering the homogenized filtrate by using a ceramic membrane, and collecting the filtrate, namely the vegetable oil material fermentation self-packaging carrier.

5. The product prepared by the preparation method of the high-stability vegetable oil material fermentation self-packaging carrier in any one of claims 1 to 4, which is characterized in that: the fermentation self-coated carrier product does not need to be additionally added with a surfactant, and fat is coated by a microbial cell membrane, has the particle size of 130-180 nm and stably exists in fermentation liquor.

6. The product of the process for preparing a high stability vegetable oil fermented self-contained carrier as set forth in claim 5, wherein: the fermented self-coated carrier product is still stably coated with the grease after centrifugation.

7. The product of the process for preparing a high stability vegetable oil fermented self-contained carrier as set forth in claim 5, wherein: the fermented self-coated carrier product is still stably coated with the grease after being placed at a high temperature of 50 ℃ for one month.

8. The product of the process for preparing a high stability vegetable oil fermented self-contained carrier as set forth in claim 5, wherein: the fermented self-coated carrier product is still stably coated with the grease after being placed for one month at 4 ℃ and 20 ℃ in sequence.

9. The product of the process for preparing a high stability vegetable oil fermented self-contained carrier as set forth in claim 5, wherein: the fermented self-coated carrier product contains protein, amino acid, polyphenol and polysaccharide.

10. The application of the product prepared by the preparation method of the high-stability vegetable oil material fermentation self-packaging carrier in the cosmetics is characterized in that: the high-stability vegetable oil material fermentation self-packaging carrier is green and safe, has no hemolytic effect, causes no adverse reaction to skin, and has no irritation.