CN109939064B - Essence containing probiotic fermentation metabolite and application of essence in cosmetics - Google Patents
Essence containing probiotic fermentation metabolite and application of essence in cosmetics Download PDFInfo
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Abstract
The invention belongs to the field of skin care products, and particularly discloses a cosmetic essence containing probiotic fermentation metabolites, a method for preparing the essence, and application of the essence containing probiotic fermentation metabolites in cosmetics, wherein the cosmetic essence comprises an active probiotic metabolite solution with the mass fraction of 10% -70%, and the balance is a base solution; the active probiotic metabolite solution is obtained by probiotic fermentation of nutrient solution containing pine tea extract and fruit extract, wherein the pine tea extract is water extract of fresh pine needles and green tea, nutrient components which are not easily absorbed by skin in the pine needles, the green tea and the fruits can be converted into substances which are easily absorbed by the skin through probiotic fermentation of the nutrient solution, functional active substances which are not contained in pure probiotic fermentation metabolites are added, and the problems of tarnish, dehydration, dryness and wrinkles of the skin can be improved and the skin can be kept tender and white by long-term use of the essence solution.
Description
Technical Field
The invention belongs to the field of skin care products, and particularly relates to essence containing probiotic fermentation metabolites, a method for preparing the essence, and application of the essence in cosmetics.
Background
The structure of the skin includes the epidermis, dermis, and subcutaneous fat. The dermis contains collagen, elastin, and other fibrous tissues. People face computers and mobile phone electronic equipment for a long time, the electronic equipment is easy to generate static electricity to attract dust or tiny particles to be attached to the surface of skin, and in addition, work and rest are irregular, the epidermis is easy to dry, and melanin precipitation is aggravated. With the age, the epidermis layer becomes thinner gradually, the metabolism of the skin is slowed down, the moisturizing factor in the dermis layer is reduced, the function of elastic fiber and collagen fiber in the dermis layer is reduced, the elasticity is weakened, and wrinkles appear; in addition, the active biological enzyme in the human body is reduced, a large number of free radicals destroy the cells of the human body, and the process of skin aging is accelerated.
On a normal skin surface, a large number of microorganisms, including microbial populations such as bacteria, fungi and viruses, are present, forming a mutually restricted and balanced microbial population. Normally, microorganisms are present orderly on the skin surface, and not only the microecological environment on the skin surface is maintained stable, but also bioactive substances used for the skin are secreted and produced.
The lactobacillus is a microorganism which survives in the intestinal tract and is beneficial to human health, and can improve the micro-ecological balance of a host, form an antibacterial biological barrier, inhibit the growth and the reproduction of pathogenic microorganisms and putrefying bacteria, and play a role in inhibiting bacteria and diminishing inflammation. More importantly, metabolites generated by lactobacillus fermentation have an important effect on improving skin, the metabolites comprise organic acid, amino acid, vitamin, exopolysaccharide, small molecular peptide and biological enzyme, the organic acid can balance the pH of the skin surface to enable the pH of the skin to be weakly acidic, and the amino acid, the vitamin and the biological enzyme can repair epidermal cells, remove pigmentation, improve rough skin to enable the skin to be bright and elastic; the microbial exopolysaccharide can also resist oxidation and delay skin aging.
For example, chinese patent CN108478459 discloses an eye essence containing lactobacillus fermentation lysis solution, which uses tea extract, pomegranate rind extract and caffeine as main frames of the eye essence for anti-aging and anti-oxidation, combines lactobacillus fermentation lysis solution to improve the absorption capacity of skin, and uses nutritional components royal jelly polypeptide, oat beta-glucan, buckwheat seed extract and ceramide as skin to supplement nutrients and water for the skin, so as to solve the problem of eye skin aging.
In addition, metabolites of lactobacillus also contain some small molecular antibacterial and antioxidant substances which can inhibit the reproduction of harmful bacteria on the surface of skin and have good anti-acne and anti-plaque effects. Chinese patent CN109498543 discloses a composition for regulating skin micro-ecological balance, which consists of a white ginseng extract, a probiotic metabolite and pearl oyster meat antioxidant peptides, a large amount of acidic substances in the probiotic metabolite are used for inhibiting the growth of harmful bacteria, and the probiotic metabolite also has the effects of removing acne cutin, reviving skin and brightening skin.
Currently, skin care products containing probiotic metabolites are in the market, but the effects of most commercial products are only limited in the publicity level. Some commercial products are added with natural extracts, such as CN108478459 which is also added with tea extract and pomegranate rind extract, however, these commercial products are simply mixed with other extracts, and it is difficult to fully utilize the characteristics of each component and element in these natural extracts and probiotic metabolites. Therefore, it is particularly important to provide a skin care substance having an excellent whitening effect, safety in use, and excellent moisturizing performance.
Disclosure of Invention
The invention aims to provide a cosmetic essence by combining the defects of the prior art, the essence contains probiotic fermented pine tea extract and metabolite of the fermented fruit extract, and has remarkable effects in repairing epidermal cells, whitening skin, moisturizing skin, removing acne and freckles.
The invention also aims to provide a method for preparing the essence, which is simple to operate, easy to control, high in production efficiency and low in cost and can be used for large-scale production.
The purpose of the invention is realized by the following technical scheme:
a cosmetic essence containing probiotic fermentation metabolites comprises an active probiotic metabolite solution with the mass fraction of 10% -70%, and the balance is a base solution; the active probiotic metabolite solution is obtained by fermenting a nutrient solution with probiotics, and the nutrient solution comprises pine tea extract and fruit extract, wherein the pine tea extract is water extract of fresh pine needles and green tea.
Preferably, the preparation method of the active probiotic metabolite solution comprises the steps of:
(1) preparing nutrient solution containing pine tea: weighing fresh pine needles and green tea, adding water into a mixture of the fresh pine needles and the green tea, boiling, filtering while the mixture is hot to obtain pine tea extract, adding a proper amount of fruits into the pine tea extract, mixing and juicing to obtain the nutrient solution;
(2) preparing a culture medium containing pine tea: adding a mixed solution of glucose, yeast powder and potassium dihydrogen phosphate into the nutrient solution, uniformly stirring, sterilizing and cooling for later use;
(3) preparation of active probiotic metabolite solution: inoculating mixed probiotic strains on the culture medium containing the pine tea, mixing, standing, fermenting, taking out, centrifuging, filtering and clarifying when the pH is reduced to be below 3.6 and the content of the superoxide dismutase reaches a set value, and thus obtaining the probiotic fermentation metabolite solution containing active substances.
In the preparation method, the mass ratio of the fruits to the fresh pine needles in the step (1) is 4-20. The effective substances in folium Pini and green tea are extracted by water extraction method, and the polyphenol, amino acid and microorganism in folium Pini and green tea can be effectively preserved, wherein the mass ratio of water to fresh folium Pini is 15-100:1, and the boiling time of water is preferably 10-30 min. In the embodiment of the invention, the fermentation temperature of the probiotics is 30-40 ℃, and the preferable temperature is 30-37 ℃. The mixed solution comprises the following components in percentage by mass: 1.0-15.0% of glucose, 0.1-5.0% of yeast powder, 0.01-1.0% of potassium dihydrogen phosphate and the balance of the nutrient solution. And (3) detecting the change of pH and bioactive substance superoxide dismutase (SOD) during probiotic fermentation, wherein if the pH is reduced more, the fermentation is finished. At the above temperature, when the pH is reduced to 3.6 or less and the content of superoxide dismutase reaches a set value, the fermentation time is preferably 6 to 24 hours.
The polyphenol compound is a mixture which has one or more aromatic rings and contains one or more hydroxyl groups, a large number of phenolic hydroxyl groups are contained in polyphenol molecules, and the phenolic hydroxyl groups are used as hydrogen donors and can capture free radicals generated in an oxidation process, inhibit or interrupt the oxidation process and balance the number of the free radicals in a matrix. The Pinaceae plants contain rich polyphenol compounds, so that the pine needles not only have high polyphenol content, but also contain more flavone and procyanidine, and are one of important antioxidant substances in the pine needles. The pine needle extract and the tea extract are only used for fermentation, instead of filtering the pine needle and the green tea residue after fermentation, so that the problem that metabolic solution is grey in the fermentation process of the pine needle can be solved.
The green tea contains tea polyphenols, caffeine, minerals, amino acids, and microorganisms. Tea polyphenols are the main components of green tea, and can provide active hydrogen for scavenging free radicals, inhibit melanin formation, and relieve pigmentation.
The fruit extract contains abundant active biological components, wherein the fruit acid can accelerate cell renewal speed, promote the detachment of dead cells, whiten skin, preserve moisture, remove wrinkles and has the function of resisting aging; vitamin C and its derivatives can reduce intermediate of melanin to inhibit melanin formation, and reduce dark oxidized melanin.
The fruit can be one or more of fresh orange, apple, mango, fresh jujube, kiwi fruit, hawthorn, grape, strawberry, crab apple, and tomato.
Preferably, the fruit is fresh orange and apple in equal proportions. The apple is rich in saccharides, vitamin C, vitamin E, inorganic salts, vitamins, organic acids, polyphenol and flavonoid compounds, the mature apple contains more catechins, procyanidine and chlorogenic acids, and can resist oxidation and aging, and the apple also contains beta-carotene which can quench singlet oxygen and remove peroxide free radicals.
The pine needle and green tea are directly boiled in water to obtain pine needle tea extract, rich polyphenol compounds are reserved in the pine needle tea extract, a culture medium prepared from the pine needle tea extract and the fruit extract is fermented by probiotics to obtain a solution containing active substances, and the solution contains rich vitamins, amino acids, enzymes, mineral substances, polypeptides, secondary metabolites, active lactic acid bacteria and the like.
Preferably, the mixed probiotics include Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus casei (Lactobacillus casei), and Lactobacillus rhamnosus (Lactobacillus. rhamnosus) species in equal proportion.
Preferably, the mass ratio of the fresh pine needles to the green tea is 5-15: 1.
Preferably, the components constituting the base liquid are as follows in mass fraction in the cosmetic essence: 2.2 to 35 percent of humectant, 0.1 to 3 percent of anti-allergic agent, 0.1 to 3.0 percent of thickening agent, 0.1 to 3 percent of emulsifier, 0.1 to 1 percent of preservative, 0.1 to 5 percent of allantoin, 0.1 to 5.0 percent of biological polypeptide and sterile water to 100 percent.
Preferably, the moisturizer comprises the following components in percentage by mass in the cosmetic essence: 0.5-5% of DPG, 260.1-5% of glycerol polyether, 0.05-2.0% of sodium polyglutamate, 0.05-2.0% of hydroxyethyl cellulose, 0.1-3.0% of HA, 0.1-2.0% of D-panthenol, 0.1-3.0% of betaine, 370.1-3.0% of ERT, 1.0-5.0% of MultiEXWaterin and 70.1-5% of starlub.
Preferably, the anti-allergic agent is selected from Chinese herbal medicine extracts. Preferably extracts of centella asiatica, polygonum cuspidatum, scutellaria baicalensis, green tea, glycyrrhiza glabra, chamomile, rosemary and other seven plants.
Preferably, the emulsifier is a water-soluble silicone wax compound. The water-soluble silicon wax compound is specifically wax-like polyether modified polydimethylsiloxane copolymer.
Preferably, the thickener is clear xanthan gum.
Preferably, the preservative is selected from compound matching, can be p-hydroxyacetophenone and A91 preservative, and the addition amount of each component is 0.1-1.0% of p-hydroxyacetophenone and 910.1-1.0% of A.
The biological polypeptide is preferably acetyl hexapeptide-8.
The invention also provides a preparation method of the essence containing the probiotic fermentation metabolite, which comprises the following steps:
(1) mixing a thickening agent, an emulsifier and sterile water, homogenizing and emulsifying, stirring at a low speed, and then defoaming;
(2) and after the temperature is reduced to a preset temperature, adding the probiotic metabolite solution containing active substances, a humectant, an anti-allergy agent, a thickening agent, a preservative, allantoin and biological polypeptide, and uniformly stirring at a low speed to obtain the essence containing the probiotic fermentation metabolite.
The invention also provides a mask containing the essence containing the probiotic fermentation metabolite. And injecting the essence containing the probiotic fermentation metabolites into a mask base cloth bag to obtain the mask containing active probiotic metabolites.
The invention also provides application of the essence containing the probiotic fermentation metabolite in cosmetics. When the essence containing the probiotic fermentation metabolites is used in different types of cosmetics, the types and contents of the components of the base liquid can be adjusted correspondingly, for example, the essence is used in products such as emollient water, emollient milk, cream, skin care essence, facial mask and the like, and the facial mask is preferred.
The invention has the beneficial effects that: the essence containing the active probiotic metabolite has strong oxidation resistance and free radical removal capacity, converts nutrient components which are not easily absorbed by skin in pine needles, green tea and fruits into substances which are easily absorbed, such as glycerol, lactic acid, lipids and the like, through probiotic fermentation, increases functional active substances such as flavonoids, anthocyanins and the like which are not contained in the pure probiotic fermentation metabolite, and improves the content of polyphenol substances. The functional active substances in the essence can accelerate the cell renewal speed and accelerate the removal of free radicals, thereby repairing epidermal cells, supplementing moisturizing factors for dermal cells and achieving the effects of moisturizing and whitening skin and keeping the skin elastic.
Detailed Description
The present invention will be described in further detail with reference to examples.
The raw materials of the invention are specifically:
the bioactive polypeptide is preferably acetyl hexapeptide-8; starlub-7 is a PEG/PPG-17/6 copolymer, available from Korea Bio Spectrum; MultiEX Waterin is a carbohydrate isomer, available from Korea Bio Spectrum; the water-soluble silicon wax compound is specifically wax-like polyether modified polydimethylsiloxane copolymer with the trade name of DMC 6038; the anti-allergic agent is selected from Chinese herbal extracts, preferably extracts of centella asiatica, polygonum cuspidatum, scutellaria baicalensis, green tea, glycyrrhiza glabra, chamomile, rosemary and other seven plants, and is purchased from Korea Bio Spectrum company under the trade name CalmYang.
Example 1
Preparing essence A containing probiotic fermentation metabolites:
(1) preparing nutrient solution containing pine tea: weighing 5.0g of fresh pine needles and 1.0g of green tea, adding the fresh pine needles and the green tea into 500mL of water, boiling for 10min to obtain pine tea liquid, adding 50.0g of fresh oranges and 50.0g of apples into the pine tea liquid, mixing, and juicing;
(2) preparing a culture medium containing pine tea: adding the following components in percentage by mass into the pine tea and orange fruit mixed nutrient solution: 1.0% of glucose, 0.1% of yeast powder and 0.01% of potassium dihydrogen phosphate, sterilizing at 105 ℃ for 10min, and cooling to 35 ℃ for later use;
(3) preparation of active probiotic metabolite solution: inoculating strains of cultured mature lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei and lactobacillus rhamnosus into the sterilized culture medium according to equal proportion, performing static fermentation at 35 ℃, fermenting for 8 hours, filtering and clarifying;
(4) preparing essence containing active substances: adding the following components in percentage by mass: 55.0% of probiotic fermentation metabolite solution, and base solution: DPG 0.2%, Starlub-70.2%, glycerol polyether-260.2%, A910.1%, sodium polyglutamate 0.05%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.5%, p-hydroxyacetophenone 0.1%, D-panthenol 0.2%, betaine 0.2%, ERT 370.1%, allantoin 0.2%, MultiEX Waterin 1.5%, CalmYang0.1%, acetyl hexapeptide-80.3%, DMC 60380.3%, and sterile water to 100%, completely dissolving the above substances in water, emulsifying, mixing well, and making into stable emulsion to obtain essence containing active substances.
Example 2
Preparing essence B containing probiotic fermentation metabolites:
(1) preparing nutrient solution containing pine tea: weighing 10.0g of fresh pine needles and 2.0g of green tea, adding the fresh pine needles and the green tea into 500mL of water, boiling for 10min to obtain pine tea liquid, adding 50.0g of fresh oranges and 50.0g of apples into the pine tea liquid, mixing, and juicing;
(2) preparing a culture medium containing pine tea: adding the following components in percentage by mass into the pine tea and orange fruit mixed nutrient solution: 1.5% of glucose, 0.2% of yeast powder and 0.01% of potassium dihydrogen phosphate, sterilizing at 105 ℃ for 10min, and cooling to 35 ℃ for later use;
(3) preparation of active probiotic metabolite solution: inoculating strains of cultured mature lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei and lactobacillus rhamnosus into the sterilized culture medium according to equal proportion, performing static fermentation at 35 ℃, fermenting for 8 hours, filtering and clarifying;
(4) preparing essence containing active substances: adding the following components in percentage by mass: 50.0% of probiotic fermentation metabolite solution, and base solution: DPG 0.1%, Starlub-70%, glyceryl polyether-260.2%, A910.1%, sodium polyglutamate 0.05%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.5%, p-hydroxyacetophenone 0.2%, D-panthenol 0.2%, betaine 0.2%, ERT 370.2%, allantoin 0.5%, MultiEX Waterin 1.0%, CalmYang0.1%, acetyl hexapeptide-80.2%, and DMC 60380.2%, and adding sterile water to 100%, dissolving the above materials in water thoroughly, emulsifying, mixing well, and making into stable emulsion to obtain essence containing active substances.
Example 3
Preparing essence C containing probiotic fermentation metabolites:
(1) preparing nutrient solution containing pine tea: weighing 20.0g of fresh pine needles and 2.0g of green tea, adding the fresh pine needles and the green tea into 500mL of water, boiling for 15min to obtain pine tea liquid, adding 60.0g of fresh oranges and 60.0g of apples into the pine tea liquid, mixing, and juicing;
(2) preparing a culture medium containing pine tea: adding the following components in percentage by mass into the pine tea and orange fruit mixed nutrient solution: 1.2% of glucose, 0.5% of yeast powder and 0.01% of potassium dihydrogen phosphate, sterilizing at 105 ℃ for 10min, and cooling to 35 ℃ for later use;
(3) preparation of active probiotic metabolite solution: inoculating strains of cultured mature lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei and lactobacillus rhamnosus into the sterilized culture medium according to equal proportion, performing static fermentation at 35 ℃, fermenting for 8 hours, filtering and clarifying;
(4) preparing essence containing active substances: adding the following components in percentage by mass: 40.0% of probiotic fermentation metabolite solution, and base solution: DPG 0.3%, Starlub-70.3%, glycerol polyether-260.2%, A910.1%, sodium polyglutamate 0.1%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.3%, p-hydroxyacetophenone 0.3%, D-panthenol 0.3%, betaine 0.3%, ERT 370.2%, allantoin 1.0%, MultiEX Waterin 1.0%, CalmYang0.1%, acetyl hexapeptide-81.5%, DMC 60380.5%, and sterile water to 100%, completely dissolving the above substances in water, emulsifying, mixing well, and making into stable emulsion to obtain essence containing active substances.
Example 4
Preparing essence D containing probiotic fermentation metabolites:
(1) preparing nutrient solution containing pine tea: weighing 25.0g of fresh pine needles and 3.0g of green tea, adding the fresh pine needles and the green tea into 500mL of water, boiling for 15min to obtain pine tea liquid, adding 60.0g of fresh oranges and 60.0g of apples into the pine tea liquid, mixing, and juicing;
(2) preparing a culture medium containing pine tea: adding the following components in percentage by mass into the pine tea and orange fruit mixed nutrient solution: 1.3 percent of glucose, 0.5 percent of yeast powder and 0.01 percent of potassium dihydrogen phosphate, sterilizing at 105 ℃ for 10min, and cooling to 35 ℃ for later use;
(3) preparation of active probiotic metabolite solution: inoculating strains of cultured mature lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei and lactobacillus rhamnosus into the sterilized culture medium according to equal proportion, performing static fermentation at 35 ℃, fermenting for 8 hours, filtering and clarifying;
(4) preparing essence containing active substances: adding the following components in percentage by mass: probiotic fermentation metabolite solution 35.0%, base solution: DPG 0.5%, Starlub-70.2%, glycerol polyether-260.3%, A910.2%, sodium polyglutamate 0.1%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.5%, p-hydroxyacetophenone 0.2%, D-panthenol 0.3%, betaine 0.3%, ERT 370.2%, allantoin 1.5%, MultiEX Waterin 1.2%, CalmYang0.1%, acetyl hexapeptide-81.5%, DMC 60380.5%, and sterile water to 100%, completely dissolving the above substances in water, emulsifying, mixing well, and making into stable emulsion to obtain essence containing active substances.
Example 5
Preparing essence E containing probiotic fermentation metabolites:
(1) preparing nutrient solution containing pine tea: weighing 30.0g of fresh pine needles and 2.5g of green tea, adding the fresh pine needles and the green tea into 500mL of water, boiling for 10min to obtain pine tea liquid, adding 100.0g of fresh oranges and 100.0g of apples into the pine tea liquid, mixing, and juicing;
(2) preparing a culture medium containing pine tea: adding the following components in percentage by mass into the pine tea and orange fruit mixed nutrient solution: 0.5% of glucose, 0.1% of yeast powder and 0.01% of potassium dihydrogen phosphate, sterilizing at 105 ℃ for 10min, and cooling to 35 ℃ for later use;
(3) preparation of active probiotic metabolite solution: inoculating strains of cultured mature lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei and lactobacillus rhamnosus into the sterilized culture medium according to equal proportion, performing static fermentation at 35 ℃, fermenting for 8 hours, filtering and clarifying;
(4) preparing essence containing active substances: adding the following components in percentage by mass: 60.0% of probiotic fermentation metabolite solution, and base solution: DPG 0.25%, Starlub-70.25%, glyceryl polyether-260.15%, A910.15%, sodium polyglutamate 0.15%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.5%, p-hydroxyacetophenone 0.25%, D-panthenol 0.35%, betaine 0.35%, ERT 370.25%, allantoin 2.5%, MultiEX Watertin 1.5%, CalmYang 0.15%, acetyl hexapeptide-81.5%, DMC 60380.5%, and sterile water to 100%, dissolving the above substances in water thoroughly, emulsifying, mixing well, and making into stable emulsion to obtain essence containing active substances.
Comparative example 1
Preparing essence F: the difference from the embodiment 1 is that the nutrient solution is obtained by squeezing green tea extract and fruits without adding fresh pine needles.
(1) Preparing a nutrient solution: weighing 1.0g of green tea, adding into 500mL of water, boiling for 10min to obtain a green tea extracting solution, adding 50.0g of fresh orange and 50.0g of apple into the green tea extracting solution, mixing and juicing;
(2) preparing a culture medium: adding the following components in percentage by mass into the nutrient solution: 1.0% of glucose, 0.1% of yeast powder and 0.01% of potassium dihydrogen phosphate, sterilizing at 105 ℃ for 10min, and cooling to 35 ℃ for later use;
(3) preparing a probiotic metabolite solution: inoculating strains of cultured mature lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei and lactobacillus rhamnosus into the sterilized culture medium according to equal proportion, performing static fermentation at 35 ℃, fermenting for 8 hours, filtering and clarifying;
(4) preparing essence: adding the following components in percentage by mass: 55.0% of probiotic fermentation metabolite solution, and base solution: DPG 0.2%, Starlub-70.2%, glyceryl polyether-260.2%, A910.1%, sodium polyglutamate 0.05%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.5%, p-hydroxyacetophenone 0.1%, D-panthenol 0.2%, betaine 0.2%, ERT 370.1%, allantoin 0.2%, MultiEX Waterin 1.5%, CalmYang0.1%, acetyl hexapeptide-80.3%, DMC 60380.3%, and sterile water to 100%, completely dissolving the above substances in water, emulsifying, mixing well, and making into stable emulsion to obtain essence containing active substances.
Comparative example 2
Preparing essence G: the difference from example 1 is that the nutrient solution containing pine tea was not subjected to probiotic fermentation treatment, and the probiotic fermentation metabolites were mixed with the nutrient solution containing pine tea as a probiotic fermentation metabolite solution containing pine tea extract.
Preparing essence A containing probiotic fermentation metabolites:
(1) preparing nutrient solution containing pine tea: weighing 5.0g of fresh pine needles and 1.0g of green tea, adding the fresh pine needles and the green tea into 500mL of water, boiling for 10min to obtain pine tea liquid, adding 50.0g of fresh oranges and 50.0g of apples into the pine tea liquid, mixing, and juicing;
(2) preparing a culture medium by the following components in percentage by mass: 1.0% of glucose, 0.1% of yeast powder and 0.01% of potassium dihydrogen phosphate, sterilizing at 105 ℃ for 10min, and cooling to 35 ℃ for later use; inoculating mature lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei and lactobacillus rhamnosus strains into a sterilized culture medium according to equal proportion, performing static fermentation at 35 ℃, fermenting for 8 hours, filtering and clarifying, and mixing probiotic fermentation metabolites with nutrient solution containing pine tea to obtain probiotic fermentation metabolites containing pine tea extract;
(3) preparing essence: adding the following components in percentage by mass: 55.0% of probiotic fermentation metabolite solution containing pine tea extract, and the basic solution: DPG 0.2%, Starlub-70.2%, glycerol polyether-260.2%, A910.1%, sodium polyglutamate 0.05%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.5%, p-hydroxyacetophenone 0.1%, D-panthenol 0.2%, betaine 0.2%, ERT 370.1%, allantoin 0.2%, MultiEX Waterin 1.5%, CalmYang0.1%, acetyl hexapeptide-80.3%, DMC 60380.3%, and sterile water to 100%, completely dissolving the above substances in water, emulsifying, mixing well, and making into stable emulsion to obtain essence containing active substances.
The essence solutions A-E obtained in the embodiments 1-5 and the preparation method are injected into a facial mask base cloth bag, and the facial mask containing the active probiotic metabolite is obtained. The cosmetic essences F to G of comparative examples 1 to 2 were treated in the same manner to prepare a mask.
Skin care test
Experimental groups: 30 patients with dull facial complexion and 30 patients with multiple wrinkles on the face were randomly divided into 3 groups, i.e., each group contained 10 patients with dull facial complexion and 10 patients with multiple wrinkles on the face. The mask corresponding to example 1, example 3 and example 5 was used. Once daily for 4 weeks, skin condition was tested for 1 day, 3 days, 7 days, 14 days, 21 days and 28 days (skin properties were characterized by skin brightness, skin elasticity values and skin texture, and specifically by VISIA skin detector) respectively.
Control group:
performing skin care tests on the facial masks prepared in the comparative examples 1-2, and marking as a comparative example 1 and a comparative example 2; the base fluid from the preparation of example 5 was used as a control and was designated as comparative example 3.
Each group of comparative examples corresponds to 10 patients with dull facial complexion and 10 patients with multiple wrinkles on the face, and skin conditions were tested for 4 weeks for 1 day, 3 days, 7 days, 14 days, 21 days, and 28 days, respectively.
The results of the above examples and comparative experiments were averaged for each group of patients. The test results are shown in the following table:
table 1 test groups skin care test results
TABLE 2 control skin care test results
According to the test result and the comparative analysis before and after use, the person with dull facial skin is obviously improved, the whiteness of the skin is obviously improved, the elasticity and the moisture of the skin are also obviously improved, and the wrinkle fading is obvious. The long-term use can improve the problems of tarnish, dehydration, dryness and wrinkles of the skin and keep the skin tender and white.
The above detailed description is specific to one possible embodiment of the present invention, and the embodiment is not intended to limit the scope of the present invention, and all equivalent implementations or modifications without departing from the scope of the present invention should be included in the technical scope of the present invention.
Claims (4)
1. The essence containing probiotic fermentation metabolites is characterized by comprising the following components in percentage by mass: 40-60% of active probiotic metabolite solution, 0.2-0.3% of DPG, 0.05-0.15% of starlub-70.2-0.3%, glycerol polyether-260.15-0.2%, A910.1-0.15%, sodium polyglutamate 0.05-0.15%, transparent xanthan gum 0.1%, hydroxyethyl cellulose 0.05%, HA 0.3-0.5%, p-hydroxyacetophenone 0.1-0.3%, D-panthenol 0.2-0.35%, betaine 0.2-0.35%, TDC-ERT 370.1-0.25%, allantoin 0.2-2.5%, MultiEX Waterin 1.0-1.5%, CalmYang 0.1-0.15%, acetyl hexapeptide-80.3-1.5%, DMC 60380.3-0.5%, sterile water to 100%;
the preparation method of the active probiotic metabolite solution comprises the following steps:
(1) preparing nutrient solution containing pine tea: weighing fresh pine needles and green tea, adding water into a mixture of the fresh pine needles and the green tea, boiling, filtering while the mixture is hot to obtain pine tea extract, adding fruits into the pine tea extract, mixing and juicing to obtain the nutrient solution; the mass ratio of the fresh pine needles to the green tea is 5-12:1, and the fruits are fresh oranges and apples in equal proportion; the mass ratio of the fruits to the fresh pine needles is 6-20: 1;
(2) preparing a culture medium containing pine tea: adding a mixed solution of glucose, yeast powder and potassium dihydrogen phosphate into the nutrient solution, uniformly stirring, sterilizing and cooling for later use;
(3) preparation of active probiotic metabolite solution: inoculating mixed probiotic strains to the culture medium containing the pine tea, performing static fermentation at 35 ℃, fermenting for 8 hours, taking out the mixture to perform centrifugal treatment when the pH is reduced to be below 3.6 and the content of superoxide dismutase reaches a set value, and filtering and clarifying to obtain a probiotic fermentation metabolite solution containing active substances;
the mixed probiotic strains consist of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei and lactobacillus rhamnosus strains in equal proportion.
2. A mask containing the essence containing the probiotic fermented metabolite of claim 1.
3. Use of the essence containing probiotic fermentation metabolites according to claim 1 for the preparation of cosmetics.
4. Use of the essence containing the probiotic fermentation metabolites in the preparation of cosmetics according to claim 3, wherein the cosmetics are facial masks.
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| CN111135123B (en) * | 2020-01-20 | 2021-08-17 | 上海应用技术大学 | Preparation method and application of selaginella tamariscina fermentation extracting solution |
| CN111321096B (en) * | 2020-03-31 | 2022-01-04 | 河南科技学院 | Method for improving bioavailability of apple juice polyphenol |
| CN114601761A (en) * | 2022-01-18 | 2022-06-10 | 科丝美诗(中国)化妆品有限公司 | Preparation method and application of plant fermentation liquor for repairing skin barrier |
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