CN109394598A - A kind of anti-acne concentrate and its application - Google Patents
A kind of anti-acne concentrate and its application Download PDFInfo
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- CN109394598A CN109394598A CN201811548217.0A CN201811548217A CN109394598A CN 109394598 A CN109394598 A CN 109394598A CN 201811548217 A CN201811548217 A CN 201811548217A CN 109394598 A CN109394598 A CN 109394598A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/9767—Pinaceae [Pine family], e.g. pine or cedar
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
The present invention provides a kind of anti-acne concentrate, the anti-acne concentrate is prepared by following methods: (1) Bacillus acidi lactici being inoculated into Japanese red pine leaf extract and fermented, obtain fermentation liquid；(2) fermentation liquid sterilizing, the filtering obtained step (1), obtained filtrate is concentrated, the anti-acne concentrate is obtained.The present invention also provides a kind of acne-removing liquids to be apt to the equal QUORASTOP of skin and anti-acne clean water.Anti-acne concentrate of the present invention, acne-removing liquid are apt to skin with anti-acne cleaning water energy by effectively inhibiting the population effects of propionibacterium acnes, can balance microorganism group effectively to achieve the effect that treat acne.Volunteer has used the product equal containing 2% kind skin, is not killing whole propionibacterium acnes, only prevents the proliferation of propionibacterium acnes, under the conditions of will not also generating bacterial drug resistance etc., acne skin can also be obtained and effectively be treated.
Description
Technical field
Present invention relates particularly to a kind of anti-acne concentrate and its applications.
Background technique
Propionibacterium acnes (scientific name: Propionibacterium acnes), are closely bound up with skin disease acne
A kind of relatively slow typical gram-positive anaerobic bacterium (rod-shaped) of growth.The normal flora for belonging to skin, generally resides in skin
In the hair follicle and sebaceous glands of skin.It reaches maturity with teen-age, keratotic plug occurs in follicular orifice, and smegma function also obviously increases
Add, because sebum contains the ingredients such as more fatty acid, be suitble to the growth and breeding of P.acnes, to become the most important disease of acne
One of because.Traditionally: propionibacterium acnes are the main bacterias for causing whelk, therefore treat the side that whelk more uses
Method is the direct mode for killing propionibacterium acnes to carry out.
But there are some researches prove: the content of actinomyces accounts for the 45.6% of bacteria total amount on healthy skin, and acne propionic acid bar
Bacterium and Propionibacterium are the one type in actinomyces.That is bacterium acidi propionici plays important role in skin health,
This discovery shows that effective acne treatment should not should be directed to pathogenic strain to kill all bacterium acidi propionicis as target
Or beneficial bacterial strain is kept or supplements, to keep the health status of skin, microorganism group is balanced to reach treatment acne
Effect.
Population effect (Quorum sensing) is a kind of bacterial community behavior manipulation for being increasingly subject to extensive concern recently
Mechanism, many bacteriums have this ability, that is, secrete one or more self-induction agent (Autoinducer), bacterium is by incuding this
A little self-induction agent judge population density and surrounding environment change, when flora number reaches certain threshold values (quorum, bacterium colony or collection
Fall number) after, start the adjusting expression of corresponding series of genes, to adjust the group behavior of thallus.Different types of bacterium has
Different population effect regulating system, many same inducers of bacterial secretory, regulates and controls the effect between variety classes bacterium with this
Behavior.There are specificities between self-induction agent and receptor for population effect system, while adjusting gene and signal transmitting system again
Diversity and complexity are embodied in system.Since pathogenic mechanism of many people or the pathogen of plant etc. is by the tune of population effect
Control, the mechanism have become the research hotspot in the fields such as medicine.Information interchange is generally acknowledged to only mostly thin between cell and cell
Occur in born of the same parents' biology, and bacterium is often purely regarded as unicellular organism.In a kind of ocean Fermi operator the 1960s
The luminescence phenomenon of (Vibrio fischeri) causes the interest of scientist, and Nealson etc. reported the bacterium in 1970 for the first time
Cell density with shine be positively correlated, Quorum sensing (Quorum- of the luminescence phenomenon by bacterium itself
Sensing System abbreviation QS system) it is controlled.
Research in recent decades confirms that bacterial CGC QS participates in the regulation of many biological functions.Wherein QS tune
The expression of disease-control opportunistic pathogen virulence factor and the formation of biomembrane are the problem of receiving much attention at present.Research confirms that pathogen is to place
The pathogenic effects of chief cell generally require the expression of virulence factor, and the pathogenic of most of pathogens is shape with biomembrane
At being closely connected together.When these pathogens form biomembrane, they are to the pathogenic of host cell and to antimicrobial
Immune performance will increase at double.
And biofilm is a kind of growth pattern of most of bacteriums in its natural state, has thallus when swimming state not
It has the advantage that.Regulation of its formation and development by intervention school-based, the system be bacterium dependent on population density and
Regulate and control a kind of mechanism of its physiological behavior.Wherein LuxS/2 type Autoinducer (autoinducer2, AI-2) intervention school-based
Also known as inter-species intervention school-based is widely present in G+ and G- bacterium.Its signaling molecule AI-2 is considered as the general letter of inter-species
Number molecule participates in the biofilm of regulation various bacteria.In addition, having now been found that a variety of LuxS/AI-2 type intervention school-baseds
Inhibitor, they can influence the formation of many bacterial biofilms.
Native country pine tree of the South Korea Korean pine as South Korea is mentioned (referred to as " east doctor in the principle and practice of oriental medicine
Treasured mirror "), the essence for possessing pine tree is so especially, so that its a part shall not be wasted.This is a kind of tool
There is the tree of powerful vitality, it is stated that insect can not infect South Korea Korean pine, and its antibacterial applications can also improve acne and be immunized
Power.And Lactobacillus saki, it is the particularly advantageous microorganism of a kind of pair of human body, it creates a kind of obstruction and control bacterial growth
Acidic environment.As an example, Lactobacillus saki can inhibit the appearance of harmful bacteria in the fermentation process of pickles, prevent pickles
It is damaged.
Acne-removing liquid prepared by the present invention is apt to the equal QUORASTOP of skin, and (butanediol, water, hydroxypropyl cyclodextrin, dipropylene glycol are red
Loose (PINUS DENSIFLORA) leaf extract, lactobacillus fermentation product) just it is being combined with antibiotic property+pure mellow wine of South Korea Korean pine
The synergistic effect of the biological preservation of lactobacillus, the product developed based on population effect.It can inhibit LuxS enzyme in bacterium
Activity；Reduce the generation of Autoinducer AI-2；Inhibit the generation of biomembrane；And it can then inhibit receptor in Skin Cell
The expression of TLR-2 and reduction TNF-α &IL-1αExpression, pass through it is this interference propionibacterium acnes intercellular communication (group
Bulk effect) come the effect of reaching controlling inflammation property acne.
Through retrieving, there are many patent about product for resolving poxes, but with few similar in mechanism of the invention.
A kind of method for improving organism surface health of Chinese patent CN105796624A discloses easily a kind of to a variety of prebiotic
The mixture of bacterium or probiotics and prebiotics is functional component, uses load in the form of cosmetics, skin care item, topical agent etc.
Body directly or indirectly changes the composition of body surface flora, and reaching improves body surface Tiny ecosystem, adjusts organism surface pathology and physiological status
Purpose.
A kind of composition that can promote skin microecological balance of Chinese patent CN103462881B and preparation method thereof is open
A kind of composition and preparation method thereof can promote skin microecological balance, the composition are few with skin prebiotics a- glucan
Sugar, fructooligosaccharide, beta glucan, plant extracts, yeast extract and the bright string coccus of natural antibacterial agent/radish root tunning filter
Liquid is as the main component.The invention composition fundamentally adjusts the microecological balance of skin, and the Maintenance Of Skin Health overcomes tradition
Cosmetics/skin care item are palliative and are also easy to produce the defect of side effect, while having moisturizing, softness, compact multiple reparation
Effect provides new thinking to solve skin problem.
A kind of unbalance prebiotic meta function formula for alleviating eczema of skin flora that adjusts of Chinese patent CN105997593A discloses
A kind of unbalance prebiotics formula for alleviating eczema of adjusting skin flora filtered out, changing formula Mars component group has Chinese mugwort
(Artemisia arygi ev.qiai) essential oil (Essential oil), inulin (Inulin) and a- glucan oligosaccharides (a-
Glucan Oligosaccharide) it is formed by different proportion, abbreviation AeIG prebiotics；AeIG can obviously prevent and adjust dermatitis
Property eczema affected skin in flora it is unbalance, inhibit the breeding of the potentially pathogenic organisms such as staphylococcus aureus, and promote bifid bar
The growth of the probiotics such as bacterium.
A kind of method for improving organism surface health of Chinese patent CN105796624A only relates to a kind of improvement organism surface
The method of health, in other words not providing specific testing in vitro data in vivo not can determine that how many additive amounts are just effective.
A kind of composition and preparation method thereof that can promote skin microecological balance of Chinese patent CN103462881B only relates to
And the microecological balance of skin is adjusted, it is not directed to the effect of inhibiting propionibacterium acnes population effect.
A kind of unbalance prebiotic meta function formula for alleviating eczema of skin flora that adjusts of Chinese patent CN105997593A only relates to
And prevent and the flora adjusted in dermatitis eczema affected skin is unbalance, inhibit the numerous of the potentially pathogenic organisms such as staphylococcus aureus
It grows, and promotes the growth of the probiotics such as Bifidobacterium, be not directed to the effect of inhibiting propionibacterium acnes population effect.
Correctly processing propionibacterium acnes strategy is the proliferation by inhibiting propionibacterium acnes, balances microorganism group
Group.
Summary of the invention
The present invention provides a kind of anti-acne concentrate and its applications, can be realized using the anti-acne concentrate and have passed through inhibition
Propionibacterium acnes population effect is come the effect of reaching anti-acne.
To achieve the above object, the technical solution taken: a kind of anti-acne concentrate, the anti-acne concentrate is by following
What method was prepared:
(1) Bacillus acidi lactici is inoculated into the aqueous solution of Japanese red pine leaf extract and is fermented, obtain fermentation liquid；
(2) fermentation liquid sterilizing, the filtering obtained step (1), obtained filtrate is concentrated, and obtains the anti-acne concentration
Object.
Preferably, the inoculum concentration of Bacillus acidi lactici is 750-850cfu/g in the step (1).It is highly preferred that the step
(1) inoculum concentration of Bacillus acidi lactici is 800cfu/g in.
Preferably, Japanese red pine leaf extract is Japanese red pine leaf water extract in the step (1).Compared to other solvent extractions, water
Extract active constituent is more abundant and safer, and what other solvents can be more or less brings certain risks or irritation into,
Cost is lower simultaneously.
Preferably, the fermentation temperature is 36.5-37.5 DEG C, and fermentation time is 47.75-48.25 hours.The fermentation temperature
Degree is 37 DEG C, and fermentation time is 48 hours.
The present invention provides a kind of acne-removing liquid, the acne-removing liquid is the addition fourth two in anti-acne concentrate described above
Alcohol, hydroxypropyl cyclodextrin and dipropylene glycol, are then filtered, and obtain filtrate, the as described acne-removing liquid.
The effect of butanediol is moisturizing, improves the anti-corrosion ability of system；Hydroxypropyl cyclodextrin is as increase active matter
Stability improves active matter bioavilability；Dipropylene glycol is the effect as solvent and moisturizing.
The present invention is by anti-acne concentrate and various solvent complexes, and preferably to go out when being free of preservative acne-removing liquid, to be apt to skin equal
The preferred solvents ratio and optimum solvent ratio of anti-corrosive properties, both effectiveness, stability and mildness, preferably, the butanediol,
Water, hydroxypropyl cyclodextrin, dipropylene glycol and anti-acne concentrate weight ratio be 49~51:42.25~43.75:3.2~3.4:
1.9~2.1:1.40~1.50.Most preferably, the butanediol, water, hydroxypropyl cyclodextrin, dipropylene glycol and anti-acne concentrate
Weight ratio is 50:43.25:3.3:2:1.45.
The present invention provides a kind of clean water of anti-acne, the clean water include anti-acne concentrate described above or on
State the acne-removing liquid.
Preferably, weight percentage of the acne-removing liquid in the clean water is 2%.
The present invention provides anti-acne concentrate described above, acne-removing liquids described above to prepare answering in product for resolving poxes
With.
Key point of the invention is that kind skin is equal (butanediol, water, hydroxypropyl cyclodextrin, dipropylene glycol, anti-acne concentrate)
Anti-acne mechanism is innovated, targetedly inhibits the population effect of propionibacterium acnes, drug resistance will not be generated while anti-acne.
Existing product for resolving poxes is most of or to kill the main means of propionibacterium acnes, in this way can be on skin
Beneficial and harmful bacteria all kills, if after deactivating a period of time can generate quick-fried acne phenomenon, while having used more also to generate resistance to
Pharmacological property；Some is the product for resolving poxes for being added to prebiotics, and the microecological balance of adjustable skin safeguards that skin is strong
Health, but this part is the microecological balance for adjusting entire skin flora, and the time needed on anti-acne effect can be longer.
The utility model has the advantages that the present invention also provides a kind of acne-removing liquid and cleansing pox removers the present invention provides a kind of anti-acne concentrate
Clean water.Anti-acne concentrate of the present invention, acne-removing liquid are apt to skin (butanediol, water, hydroxypropyl cyclodextrin, dipropylene glycol, acne concentrate)
With anti-acne cleaning water energy by effectively inhibit propionibacterium acnes population effect, can effectively balance microorganism group to
Achieve the effect that treat acne.Volunteer has used the product equal containing 2% kind skin, is not killing whole acne propionic acid bars
Bacterium only prevents the increment of propionibacterium acnes, and under the conditions of will not also generating bacterial drug resistance etc., acne skin can also be obtained effectively
Treatment.
Detailed description of the invention
Fig. 1 is the influence that acne-removing liquid of the present invention generates AI-2.
Fig. 2 is influence of the acne-removing liquid of the present invention to LuxS enzymatic activity.
Fig. 3 is influence of the acne-removing liquid of the present invention to biofilm formation.
Fig. 4 is the influence that acne-removing liquid of the present invention expresses TLR-2.
Fig. 5 is influence of the acne-removing liquid of the present invention to TNF-α, IL-1 alpha expression.
Fig. 6 is influence of the acne-removing liquid of the present invention to acne skin.
Specific embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
The present embodiment anti-acne concentrate the preparation method is as follows:
(1) Bacillus acidi lactici is inoculated into the aqueous solution of Japanese red pine leaf extract with the inoculum concentration of 800cfu/g and is fermented at 37 DEG C
48 hours, obtain fermentation liquid；
(2) fermentation liquid sterilizing, the filtering obtained step (1) filters out the Bacillus acidi lactici and others macromolecular of inactivation
Obtained filtrate is concentrated, obtains concentrate, the as described anti-acne concentrate by metabolic waste.
The present embodiment acne-removing liquid be apt to skin it is equal the preparation method is as follows:
Butanediol, water, hydroxypropyl cyclodextrin and dipropylene glycol are added in the present embodiment anti-acne concentrate, then filters,
Filtrate is obtained, the as described acne-removing liquid.
Embodiment 2
It is kind that the present invention preferably goes out the acne-removing liquid when being free of preservative by anti-acne concentrate and different solvents compounded combination
The skin anti-corrosive properties of (butanediol, water, hydroxypropyl cyclodextrin, dipropylene glycol, anti-acne concentrate), both effectiveness, stability and mild
Property optimum solvent ratio, test process such as the following table 1:
1 compound scheme of table
1, stability test:
Test group 1: stability test passes through but preservative challenge testing does not pass through.
Test group 2: stability test passes through but preservative challenge testing does not pass through.
Test group 3: stability test passes through and preservative challenge testing passes through.
Test group 4: stability test does not pass through but preservative challenge testing passes through.
Test group 5: stability test passes through and preservative challenge testing passes through.
Test group 6: stability test passes through and preservative challenge testing passes through.
Test group 7: stability test passes through and preservative challenge testing passes through.
Test group 8: stability test passes through and preservative challenge testing passes through.
Stability is chosen from the above test and test group 3, test group 5, test group 6, test that preservative challenge testing passes through
The preferred proportion group of group 7 and test group 8 carries out skin irritation test again:
2, test method: chicken embryo experiment
Test concentrations: above-mentioned test group is diluted to the solution of the acne-removing liquid containing 10wt%.
Test result is by allergy classification of severity: I grade (severe allergy) II grade (obvious allergy) III grade (moderate allergy)
IV grade (slight allergy) V (no allergy)
Test result is as follows table 2:
2 test result of table
| Test group | Test result |
| Test group 3 | V |
| Test group 5 | V |
| Test group 6 | IV |
| Test group 7 | V |
| Test group 8 | V |
Preferred group is tested above are as follows: test group 3, test group 5, test group 7 and test group 8
From preferred group above: test group 3, test group 5, test group 7 and test group 8 carry out both effectiveness test:
Using the test method (it is literary that the specific test method is as follows) of testing in vitro:
Test result is as follows table 3:
3 test result of table
The above test obtains preferred group best are as follows: test group 3: butanediol: water: hydroxypropyl cyclodextrin: dipropylene glycol: anti-acne
Concentrate=50:43.25:3.3:2:1.45.
The test process of test group 3 is specific as follows:
1. testing in vitro:
A) inhibit the generation of AI-2
Test method: under the conditions of kind skin is existing, propionibacterium acnes are cultivated 37 DEG C in 72 hours, anaerobic item
Part.AI-2 is measured by Elman analytic approach.As a result as shown in Figure 1:
AI-2 is a member in the signaling molecule family used in quorum sensing from object is lured.
The yield of AI-2 reduces 45%
Inhibit the generation of AI-2
B) inhibit the activity of LuxS enzyme
Test method: it under the conditions of kind skin is existing, cultivates propionibacterium acnes and SAH reacts 1 hour at 37 DEG C, nothing
Oxygen condition.LuxS activity is measured by Elman analytic approach.
Ribose homocysteine lyase (LuxS) participates in AI-2 from the synthesis for luring object, this plays weight in population effect
It acts on.
As a result as shown in Figure 2: LuxS activity reduces 49%
C the biomembrane of propionibacterium acnes) is inhibited to generate:
Test method: (37 DEG C, carbon dioxide: 5%) exist human epidermal keratinocyte (HaCaT) culture solution within 48 hours
It is cultivated with the culture medium of propionibacterium acnes culture medium.The expression of mRNA is analyzed by RT-PCR.
Biomembrane plays an important role in the pathogenesis of many human infections.Propionibacterium acnes can be each
The drug resistant biomembrane of height is formed on kind biomaterial.As a result as shown in Figure 3:
Kind skin inhibits the 38% of biofilm formation
D) the anti-inflammatory effect of Skin Cell:
Test method: (37 DEG C, carbon dioxide: 5%) exist human epidermal keratinocyte (HaCaT) culture solution within 48 hours
It is cultivated in the culture medium of propionibacterium acnes.With the expression of western blot analysis protein.
About Toll-like receptor TLR-2
It is a kind of protein, it plays key effect in innate immune system.
By stimulation TLR-2, innate immune system can identify microbe composition, then secretion inducing cell factor/become
Change the factor.
As a result as shown in Figure 4:
Kind skin inhibits TLR-2 to express
With reference to: * J Immunol.2002 Aug 1；169(3):1535-41.
–Activation of toll-like receptor 2 in acne triggers inflammatory
cytokine responses.
- E) Skin Cell anti-inflammatory effect:
Test method: (37 DEG C, carbon dioxide: 5%) exist human epidermal keratinocyte (HaCaT) culture solution within 48 hours
It is cultivated in the culture medium of propionibacterium acnes.The expression of mRNA is analyzed by RT-PCR.
Tumor necrosis factor (TNF-α) is a kind of cell signaling protein (cell factor), is related to systemic inflammatorome.
Interleukin (IL-1 α) is responsible for the generation of inflammation, and promotes fever and septicemia.
As a result as shown in Figure 5:
Kind skin reduces
The expression of TNF-α: 50% ↓
The expression of-IL-1 α: 71%
2. internal test:
Improve acne skin:
Volunteer: male and female of 8 ages between 14 years old to 27 years old.
Formula: clean water, it is equal containing 2% kind skin.
Application method: 14 days twice daily.
Analysis method: comparison of taking pictures (before and after use)
As a result as shown in Figure 6: acne-removing liquid of the present invention is apt to skin and significantly improves acne skin.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention
The limitation of range is protected, although the invention is described in detail with reference to the preferred embodiments, those skilled in the art should
Understand, it can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the essence of technical solution of the present invention
And range.
Claims (10)
1. a kind of anti-acne concentrate, which is characterized in that the anti-acne concentrate is prepared by following methods:
(1) Bacillus acidi lactici is inoculated into the aqueous solution of Japanese red pine leaf extract and is fermented, obtain fermentation liquid；
(2) fermentation liquid sterilizing, the filtering obtained step (1), obtained filtrate is concentrated, the anti-acne concentrate is obtained.
2. anti-acne concentrate according to claim 1, which is characterized in that the inoculum concentration of Bacillus acidi lactici in the step (1)
For 750-850cfu/g；Preferably, the inoculum concentration of Bacillus acidi lactici is 800cfu/g in the step (1).
3. anti-acne concentrate according to claim 1, which is characterized in that Japanese red pine leaf extract is red in the step (1)
Pine needle water extract.
4. anti-acne concentrate according to claim 1, which is characterized in that the fermentation temperature is 36.5-37.5 DEG C, fermentation
Time is 47.75-48.25 hours；Preferably, the fermentation temperature is 37 DEG C, and fermentation time is 48 hours.
5. a kind of acne-removing liquid, which is characterized in that the acne-removing liquid is in the anti-acne concentrate as described in as any such as claim 1-4
Butanediol, hydroxypropyl cyclodextrin and dipropylene glycol is added, then filters, obtains filtrate, the as described acne-removing liquid.
6. acne-removing liquid according to claim 5, which is characterized in that the butanediol, water, hydroxypropyl cyclodextrin, double third sweet
The weight ratio of alcohol and anti-acne concentrate is 49~51:42.25~43.75:3.2~3.4:1.9~2.1:1.40~1.50.
7. acne-removing liquid according to claim 5, which is characterized in that the butanediol, water, hydroxypropyl cyclodextrin, double third sweet
The weight ratio of alcohol and anti-acne concentrate is 50:43.25:3.3:2:1.45.
8. a kind of clean water of anti-acne, which is characterized in that the clean water includes that the anti-acne as described in claim 1-4 is any is dense
Contracting object or the acne-removing liquid as described in claim 5-7 is any.
9. clean water according to claim 7, which is characterized in that weight percent of the acne-removing liquid in the clean water
Content is 2%.
10. prepared by the anti-acne concentrate as described in claim 1-4 is any, the acne-removing liquid as described in claim 5-7 is any
Application in product for resolving poxes.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811548217.0A CN109394598B (en) | 2018-12-17 | 2018-12-17 | Acne removing concentrate and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811548217.0A CN109394598B (en) | 2018-12-17 | 2018-12-17 | Acne removing concentrate and application thereof |
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| CN109394598B CN109394598B (en) | 2021-07-09 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109939064A (en) * | 2019-05-15 | 2019-06-28 | 哈尔滨美华生物技术股份有限公司 | The Essence of the metabolite containing probiotics fermention and its application in cosmetics |
| CN110025538A (en) * | 2019-05-23 | 2019-07-19 | 广东芭薇生物科技股份有限公司 | It is a kind of to improve skin acne, the composition of inflammation and its application |
| CN110897911A (en) * | 2019-11-21 | 2020-03-24 | 广州市科能化妆品科研有限公司 | Novel anti-acne composition and application thereof in cosmetics |
| CN112472653A (en) * | 2020-12-18 | 2021-03-12 | 广州澳梓美生物科技有限公司 | Acne-removing aloe gel and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090002678A (en) * | 2007-07-02 | 2009-01-09 | 주식회사 엘지생활건강 | Cosmetic composition for preventing of kin trouble |
| KR20110075626A (en) * | 2009-12-28 | 2011-07-06 | (주) 젠셀 | Composition for promoting hair growth comprising fermented oriental medicine extract |
-
2018
- 2018-12-17 CN CN201811548217.0A patent/CN109394598B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090002678A (en) * | 2007-07-02 | 2009-01-09 | 주식회사 엘지생활건강 | Cosmetic composition for preventing of kin trouble |
| KR20110075626A (en) * | 2009-12-28 | 2011-07-06 | (주) 젠셀 | Composition for promoting hair growth comprising fermented oriental medicine extract |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109939064A (en) * | 2019-05-15 | 2019-06-28 | 哈尔滨美华生物技术股份有限公司 | The Essence of the metabolite containing probiotics fermention and its application in cosmetics |
| CN109939064B (en) * | 2019-05-15 | 2020-09-29 | 哈尔滨美华生物技术股份有限公司 | Essence containing probiotic fermentation metabolite and application of essence in cosmetics |
| CN110025538A (en) * | 2019-05-23 | 2019-07-19 | 广东芭薇生物科技股份有限公司 | It is a kind of to improve skin acne, the composition of inflammation and its application |
| CN110025538B (en) * | 2019-05-23 | 2022-01-28 | 广东芭薇生物科技股份有限公司 | Composition for improving skin acne and inflammation and application thereof |
| CN110897911A (en) * | 2019-11-21 | 2020-03-24 | 广州市科能化妆品科研有限公司 | Novel anti-acne composition and application thereof in cosmetics |
| CN112472653A (en) * | 2020-12-18 | 2021-03-12 | 广州澳梓美生物科技有限公司 | Acne-removing aloe gel and preparation method thereof |
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