CN109010140A - A kind of fermentation grease and preparation method thereof, purposes - Google Patents
A kind of fermentation grease and preparation method thereof, purposes Download PDFInfo
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- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The invention discloses a kind of fermentation greases and preparation method thereof, purposes；The present invention prepares glyceride using microorganism, and wall breaking technology is recycled to extract glyceride, obtains the stable grease of content of fatty acid, the basic grease as next step fermentation；Fermentation is remixed after the vegetable fat such as addition different proportion grape seed oil, wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil, the fermentation grease being prepared is safe, non-stimulated, with good skin sense, excellent oxidation resistance, good oxidation stability can repair skin barrier and significantly improve skin repair ability.
Description
Technical field
The present invention relates to cosmetic technical fields, and in particular to a kind of fermentation grease and preparation method thereof, purposes.
Background technique
The skin of health is bright and clean and flexible, and the function of these appearance marks and skin moisture-keeping system itself has
It closes, the function of moisture-keeping system is the water content for maintaining skin certain, and resistance does evaporation caused by skin as internal and external factor
The skin damages such as dry.Grease is as a kind of skin moisturizer, it is possible to reduce skin water loss protects skin barrier, improves skin
Skin drying regime, hence it is evident that improve the water content of skin, increase skin elasticity, prevent skin damage and promote damaged skin reparation.
Active oxygen as active oxygen refer to chemical property it is active containing oxygen atom or atomic group, as superoxide radical (
O2 -), hydrogen peroxide (H2O2), hydroxy radical (OH) etc., active oxygen can make the unsaturated fatty acid in lipoid that peroxide occur
Change reaction, the attack cells film in organism makes to lose cell function, and prevents cytothesis.The intracorporal active oxygen of people be by
What the reasons such as pressure, ultraviolet light, atmosphere pollution, smoking generated.Excessive active oxygen can destroy the collagen egg for giving skin elastic
White and elastin laminin, so as to cause wrinkle, relaxation.Therefore at present to it is this can inhibit cause aging active oxygen it is reactive
The attention rate of antioxidant gradually draws increase.
Vegetative grease is in Cosmetic Market using relatively broad, all being such as olive oil, jojoba oil, grape seed oil
Common vegetable fat in cosmetic.Since the fatty acid proportion of various vegetable fat is different, the moisturizing of each vegetable fat
Power, skin sense, oxidation stability are also different.
General so-called ferment oil, refers to the Chinese medicines such as fermented ginseng, and extract liposoluble constituent come make an addition in oil or
The Chinese medicines such as ginseng are added in oil to ferment.Chinese patent CN107467207A discloses a kind of preparation side of vegetable fat
Method, using a kind of fermenting plant grease in monascus (red yeast rice category) fermentation preparation, but without reference to the tool of vegetable fat after fermentation
Body index and using effect.Chinese patent CN106420404A discloses the fermenting plant grease with antioxidant effect
Cosmetic compositions are fermented under the effect of Basidiomycetes Pseudozima microorganism belonging to genus using the vegetable oil of several different proportions, are obtained
To the plant fermentation oil that can be used in cosmetics.Chinese patent CN106619231A discloses a kind of system of fermenting plant grease
Macromolecular in grease is resolved into small molecule by control yeast or lactic acid bacteria fermentation process by Preparation Method.But above-mentioned patent
It not can solve following problems.First, the above invention is not adjustable the fatty acid proportion after fermenting in grease；Second, the above invention
It cannot cannot improve the oil oxidative stability of grease after fermenting；Third, the above invention is using vegetable fat as fermentation substrate, cost
It is higher；Third, the vegetable fat invented above not can relate to repair the effect of skin barrier；Fourth, the vegetable oil invented above
Effect of the rouge without reference to skin repair ability.
Summary of the invention
It is an object of the invention to overcome above-mentioned prior art problem, a kind of fermentation grease and preparation method thereof is provided, is used
On the way.The present invention first prepares glyceride using volume branch Mucor fermentation, the glyceride that fermentation generates then is extracted, with grape seed oil, small
The vegetative greases such as wheat germ oil, jojoba seed oil, camellia seed oil, avocado oil form compound lard, recycle tinder fungus
It ferments, obtains a kind of fermentation grease；The fermentation grease is safe, non-stimulated, has good skin sense, excellent anti-oxidant energy
Power, good oxidation stability can repair skin barrier and significantly improve skin repair ability, can be widely applied to
In cosmetic.
Fermentation grease of the invention include the grease obtained using microbial fermentation and grape seed oil, wheat-germ oil,
The vegetative greases such as jojoba seed oil, camellia seed oil, avocado oil.Ratio of components range for each oil of above-mentioned fluid composition is
Refer to, in order to the stability of grease, effect and be suitable for cosmetics optimal oil ratio of components, thus table within the above range
Show optimal effect.
The purpose of the present invention is achieved through the following technical solutions:
The present invention relates to a kind of fermentation grease, the fermentation grease is to be prepared by a method comprising the following steps and obtain:
S1, to roll up branch Mucor fermentation medium as culture substrate, be inoculated with Mucor circinelloides kind；
S2, filtering obtain volume branch Mucor fungi wet cell, different for the addition of 1:8~10 with isopropanol mass ratio according to fungi
Propyl alcohol impregnates 8~10h；Filter again, the mass ratio according to fungi and 0.2mol/L, pH8.0 kaliumphosphate buffer be 1:15~
The 20 addition kaliumphosphate buffers, 28~32 DEG C of 1.5~2h of stirring；It is separated by filtration, obtains upper layer oily liquids；
Oily liquids, grape seed oil, wheat-germ oil, jojoba seed oil, camellia seed oil, crocodile obtained in S3, step S2
Pear oil is mixed for 70~75:5~10:5~10:5~10:5~10:5~10 in mass ratio, obtains compound lard；
S4, under aerobic condition, in culture solution cultivate shelf fungus be proliferated；The culture is carried out at 28~30 DEG C
Fermentation in 48~72 hours；
S5, complete culture shelf fungus proliferation culture solution in add the compound lard, using the compound lard as
Culture medium ferments again；
S6, the grease after step S5 fermentation is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 95~96 DEG C, i.e.,
Obtain the fermentation grease.
Preferably, in step S1, inoculum concentration 8%-10%, fermentation temperature at 28~30 DEG C, fermentation time 60~
84h stirs 300~400r/min, pH 6.0~6.5.
Preferably, the volume branch Mucor fermentation medium containing glucose 40.0%, yeast extract 3.0%, corn pulp 10.0%,
MgSO41.0%, FeSO40.2%, NH4Cl 3.0%, K2PO43.0%；PH is 6.0-6.5.
Preferably, the Mucor circinelloides kind is Mucor circinelloides kind CGMCC 3.14862.
Preferably, in step S4, the shelf fungus is selected from tinder fungus, heart rot (P.gilvus), fire wood
One or more of shelf fungus (P.igniarius), phellinus linteus (P.linteus), Phellinus pini (P.pini).
It is further preferred that the shelf fungus is tinder fungus CGMCC 5.1494.
Preferably, in step S4, the culture solution uses common production medium, if culture medium prescription is glucose
3.0%, peptone 1.0%, yeast extract 0.5%, NaNO30.1%, K2HPO40.1%, KCl 0.05%, FeSO4
0.001%；PH value is 6.5~7.0.
Preferably, in step S5, the fermentation is the fermentation carried out at 30~32 DEG C 48~72 hours, in fermentation process
Acid value is controlled 0.2~0.5.
In above-mentioned preparation method, step S1 prepares glyceride using microorganism, and wall breaking technology is recycled to extract glyceride；?
Drying, organic solvent is not used to obtain triglycerides in the case of extracting, the basic grease as next step fermentation grease.
In step S3, for jojoba seed oil by the oil that is obtained in the form of cold pressing from Jojoba fruit, grease is predominantly straight
Chain grease, chemical structure is similar to sebum ingredient, is easily impregnated in skin, have skin moisturization, can reduce wrinkle of skin,
Prevent chapped skin and other effects.
Camellia seed oil includes the nutrition such as squalene abundant, sterol, vitamin E as the oil extracted from the seed of camellia tree
Ingredient, antioxidation is prominent and is unlikely to deteriorate.Camellia seed oil, oleic acid content up to 70-80% there is stronger antioxygen to be turned into
With.
Grape seed oil is obtained by crushing grapes seed, the Asia oil of vitamin E and high level containing antioxidant
Acid.It is easy to permeate in skin depths, and has moistening effect outstanding.
Avocado oil is to extract to be made from the fruit of avocado, and appearance has fluorescence, is rich in various vitamins, sterol, lecithin
The effective components such as rouge, the oleic acid in fatty acid is up to 60%.With preferable lubricity, mildness, stability, to inflammation
Disease, acne have certain curative effect.
Wheat-germ oil contain there are many amino acid, sitosterol, lecithin, allantoin, arginine, vitamin A, B, D, E with
And the nutritional ingredients such as beta carotene, gamma oryzanol.Can skin care, and skin, hair aging can be prevented.
In step S5, fermentation grease of the invention is characterized in that, the lower fermentation efficiency of the acid value of the grease to ferment
Higher, therefore, acid value control is for 0.2~0.5 range.
The invention further relates to a kind of fermentation greases of the invention to improve the purposes in skin sense function cosmetics in preparation.
The invention further relates to a kind of fermentation greases of the invention to prepare the purposes in anti-oxidation function cosmetics.According to this
The fermentation grease that the preparation method obtains is invented, there is antioxidant effect.Experimental example according to the present invention, can be confirmed in root
In the case where ferment oil according to grease composition preparation of the invention, with azymic miscella and not by the hair of above-mentioned composition preparation
Ferment oil is compared, and free radical elimination ability is higher, shows antioxidant effect outstanding, to have the production that can inhibit because of active oxygen
The effect of raw antioxidant.
The invention further relates to a kind of fermentation greases of the invention to have effects that the cosmetics of oil oxidative stability in preparation
In purposes.Fermentation grease according to the present invention, above-mentioned fermentation grease be characterized in that, above-mentioned ferment oil oil oxidative stability
It is excellent.Experimental example according to the present invention, can be confirmed grease according to the present invention composition preparation ferment oil in the case where, with
Azymic miscella and not by above-mentioned composition prepare ferment oil compare, oil oxidative stability can be improved, extend grease
Shelf life.
The invention further relates to a kind of fermentation greases of the invention to repair the purposes in skin barrier function cosmetics in preparation.
Fermentation grease according to the present invention, above-mentioned fermentation grease are characterized in that above-mentioned fermentation Oily skin has excellent skin barrier
Repair effect.Experimental example according to the present invention can be confirmed the case where grease according to the present invention forms the ferment oil of preparation
Under, with azymic miscella and not by the ferment oil of above-mentioned composition preparation compared with, skin barrier repairing effect can be improved.
The invention further relates to a kind of fermentation greases of the invention in preparation anti-inflammatory cosmetics, antipruritic cosmetics or skin repair
Purposes in function cosmetics.Fermentation grease according to the present invention, above-mentioned fermentation grease are characterized in that above-mentioned ferment oil tool disappears
Scorching itching-relieving action, hence it is evident that improve skin repair ability.Experimental example according to the present invention can be confirmed in grease according to the present invention
In the case where the ferment oil for forming preparation, with azymic miscella and not by the ferment oil of above-mentioned composition preparation compared with, tool disappears
Scorching itching-relieving action, hence it is evident that improve skin repair ability.
Preferably, the dosage form of the cosmetics is selected from toner, lotion, massage cream, nourishing cream, hand lotion, soothing oil, essence
Hua Su, facial mask, washing cream, skin cream, shower cream.
Compared with prior art, the invention has the following beneficial effects:
(1) present invention fermentation grease prepares glyceride using microorganism, recycles wall breaking technology to extract glyceride, obtains
The stable grease of content of fatty acid, the basic grease as next step fermentation；Hair is remixed after addition different proportion vegetable fat
Ferment, the process can adjust the physico-chemical properties of compound lard, can obtain the compound lard than not fermenting after the fermentation has been completed
Superior new oil in terms of anti-oxidant, stability, skin；
(2) fermentation grease prepared by the present invention is the experiment proved that safe, non-stimulated；
(3) fermentation grease prepared by the present invention, oil oxidative stability is excellent, and shelf life is long.
(4) fermentation grease prepared by the present invention, oxidation resistance are strong；
(5) it is excellent to repair skin barrier function for fermentation grease prepared by the present invention；
(6) fermentation grease prepared by the present invention, can play the role of anti-inflammatory anti-itch, hence it is evident that improve skin repair ability；
(7) preparation method that the present invention uses uses less vegetable fat than existing invention, effectively save can utilize
Plant resources, and cost of material is low, and entire production process all uses cold fermentation, and it is toxic not add other for mild condition
Harmful substance, added value of product are high, it is easy to accomplish large-scale production；
(8) products of the present invention can be widely applied to cosmetic industry, wide market.
Specific embodiment
The following describes the present invention in detail with reference to examples.Following embodiment will be helpful to those skilled in the art
The present invention is further understood, but the invention is not limited in any way.It should be pointed out that those skilled in the art
For, without departing from the inventive concept of the premise, it can also make certain adjustments and improvements.These belong to guarantor of the invention
Protect range.
Embodiment 1 prepares glyceride, addition vegetable oil fermentation using microorganism
1) to roll up branch Mucor fermentation medium (containing glucose 40.0%, yeast extract 3.0%, corn pulp 10.0%, MgSO4
1.0%, FeSO40.2%, NH4Cl 3.0%, K2PO43.0%, PH6.0-6.5) it is culture substrate, inoculation is rolled up in the fermenter
Branch mucor strain (preferred Mucor circinelloides kind CGMCC 3.14862 in the present embodiment；Other mucor strains can also be used), inoculation
Amount is 10%, and fermentation temperature is at 30 DEG C, and fermentation time is in 72h；Stir 400r/min；pH 6.0；
2) it filters, obtains Mucor fungi wet cell, add isopropyl than 1:8 according to fungi quality (g) and isopropanol quality (g)
Alcohol impregnates 8h；It filters again, (contains glucose according to fungi quality (g) and 0.2mol/L, pH8.0 kaliumphosphate buffer
40.0%, yeast extract 3.0%, corn pulp 10.0%, MgSO41.0%, FeSO40.2%, NH4Cl 3.0%, K2PO43.0%,
PH6.0-6.5) quality (g) adds kaliumphosphate buffer, 28 DEG C of stirring 1.75h than 1:15；It is separated by filtration, obtains upper layer oily liquid
Body；
3) grease, grape seed oil obtained in step 2), wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil,
Content ratio is 70:5:10:5:5:5, is mixed according to a certain percentage；
4) cultivating tinder fungus (CGMCC 5.1494) under aerobic condition, in culture solution (also can be selected and belongs to (layer hole
Pseudomonas) heart rot (P.gilvus), phelliuns igniarius (P.igniarius), phellinus linteus
(P.linteus), Phellinus pini (P.pini)) it is proliferated；Fermentation can carry out 72 hours hairs under the conditions of 28 DEG C of temperature
Ferment, using common production medium, (e.g., culture medium prescription is glucose 3.0%, peptone 1.0%, yeast extract to culture solution
0.5%, NaNO30.1%, K2HPO40.1%, KCl 0.05%, FeSO40.001%, pH value 6.5-7.0)；
5) compound lard made from step 3) is added in the culture solution for completing culture tinder fungus proliferation, with miscella
Rouge carries out third time fermentation as culture medium again.Wherein, third time fermentation can carry out fermentation in 72 hours under the conditions of 30 DEG C.
Acid value control terminates third time and ferments 0.2~0.5.
6) grease after third time being fermented is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 95 DEG C.
Embodiment 2 prepares glyceride, addition vegetable oil fermentation using microorganism
1) to roll up branch Mucor fermentation medium as culture substrate, it is inoculated with Mucor circinelloides kind in the fermenter, inoculum concentration is
10%, fermentation temperature is at 28 DEG C, and fermentation time is in 60h；Stir 300r/min；pH 6.0；
2) it filters, obtains volume branch Mucor fungi wet cell, add with isopropanol quality (g) than 1:10 according to fungi quality (g)
Add isopropanol, impregnates 8h；It filters again, according to fungi quality (g) and 0.2mol/L, pH8.0 kaliumphosphate buffer quality (g) ratio
1:20 adds kaliumphosphate buffer, 31 DEG C of stirring 1.5h；It is separated by filtration, obtains upper layer oily liquids；
3) grease, grape seed oil obtained in step 2), wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil,
Content ratio is 70:7:8:5:7:8, is mixed according to a certain percentage；
4) tinder fungus (CGMCC 5.1494) is cultivated under aerobic condition, in culture solution to be proliferated；Fermentation can be
Under the conditions of 28 DEG C of temperature, fermentation in 48 hours is carried out, culture solution uses common production medium；
5) compound lard is added in the culture solution for completing culture tinder fungus proliferation, using compound lard as culture medium
Third time fermentation is carried out again.Wherein, third time fermentation can carry out fermentation in 48 hours under the conditions of 30 DEG C.Acid value is controlled 0.2
~0.5, terminate third time and ferments.
6) grease after third time being fermented is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 95 DEG C.
Embodiment 3 prepares glyceride, addition vegetable oil fermentation using microorganism
1) to roll up branch Mucor fermentation medium as culture substrate, it is inoculated with Mucor circinelloides kind in the fermenter, inoculum concentration is
10%, fermentation temperature is at 30 DEG C, and fermentation time is in 84h；Stir 350r/min；pH 6.5；
2) it filters, obtains volume branch Mucor fungi wet cell, add with isopropanol quality (g) than 1:10 according to fungi quality (g)
Add isopropanol, impregnates 10h；It filters again, according to fungi quality (g) and 0.2mol/L, pH8.0 kaliumphosphate buffer quality (g)
Kaliumphosphate buffer, 30 DEG C of stirring 2h are added than 1:18；It is separated by filtration, obtains upper layer oily liquids；
3) grease, grape seed oil obtained in step 2), wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil,
Content ratio is 75:5:5:5:5:5, is mixed according to a certain percentage；
4) tinder fungus (CGMCC 5.1494) is cultivated under aerobic condition, in culture solution to be proliferated；Fermentation can be
Under the conditions of 30 DEG C of temperature, fermentation in 72 hours is carried out, culture solution uses common production medium；
5) compound lard is added in the culture solution for completing culture tinder fungus proliferation, using compound lard as culture medium
Third time fermentation is carried out again.Wherein, third time fermentation can carry out fermentation in 72 hours under the conditions of 32 DEG C.Acid value is controlled 0.2
~0.5, terminate third time and ferments.
6) grease after third time being fermented is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 96 DEG C.
Embodiment 4 prepares glyceride, addition vegetable oil fermentation using microorganism
1) to roll up branch Mucor fermentation medium as culture substrate, it is inoculated with Mucor circinelloides kind in the fermenter, inoculum concentration is
10%, fermentation temperature is at 30 DEG C, and fermentation time is in 75h；Stir 320r/min；pH 6.2；
2) it filters, obtains volume branch Mucor fungi wet cell, added according to fungi quality (g) and isopropanol quality (g) than 1:9
Isopropanol impregnates 8.5h；It filters again, according to fungi quality (g) and 0.2mol/L, pH8.0 kaliumphosphate buffer quality (g) ratio
1:17 adds kaliumphosphate buffer, 32 DEG C of stirring 1.8h；It is separated by filtration, obtains upper layer oily liquids；
3) grease, grape seed oil obtained in step 2), wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil,
Content ratio is 72:5:5:5:5:8, is mixed according to a certain percentage；
4) tinder fungus (CGMCC 5.1494) is cultivated under aerobic condition, in culture solution to be proliferated；Fermentation can be
Under the conditions of 28.5 DEG C of temperature, fermentation in 60 hours is carried out, culture solution uses common production medium.
5) compound lard is added in the culture solution for completing culture tinder fungus proliferation, using compound lard as culture medium
Third time fermentation is carried out again.Wherein, third time fermentation can carry out fermentation in 64 hours under the conditions of 31 DEG C.Acid value is controlled 0.2
~0.5, terminate third time and ferments.
6) grease after third time being fermented is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 95.5 DEG C.
Embodiment 5 prepares glyceride, addition vegetable oil fermentation using microorganism
1) to roll up branch Mucor fermentation medium as culture substrate, it is inoculated with Mucor circinelloides kind in the fermenter, inoculum concentration is
10%, fermentation temperature is at 28.5 DEG C, and fermentation time is in 80h；Stir 380r/min；pH 6.4；
2) it filters, obtains volume branch Mucor fungi wet cell, add with isopropanol quality (g) than 1:8.5 according to fungi quality (g)
Add isopropanol, impregnates 9.5h；It filters again, according to fungi quality (g) and 0.2mol/L, pH8.0 kaliumphosphate buffer quality (g)
Kaliumphosphate buffer, 29 DEG C of stirring 1.6h are added than 1:18.5；It is separated by filtration, obtains upper layer oily liquids；
3) grease, grape seed oil obtained in step 2), wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil,
Content ratio is 71:9:5:5:5:5, is mixed according to a certain percentage；
4) tinder fungus (CGMCC 5.1494) is cultivated under aerobic condition, in culture solution to be proliferated；Fermentation can be
Under the conditions of 29.5 DEG C of temperature, fermentation in 68 hours is carried out, culture solution uses common production medium.
5) compound lard is added in the culture solution for completing culture tinder fungus proliferation, using compound lard as culture medium
Third time fermentation is carried out again.Wherein, third time fermentation can carry out fermentation in 72 hours under the conditions of 32 DEG C.Acid value is controlled 0.2
~0.5, terminate third time and ferments.
6) grease after third time being fermented is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 95.5 DEG C.
Comparative example 1, the grease for not preparing glyceride using microorganism and not fermenting (ratio is same as Example 1)
Preparation
Grape seed oil, wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil source oil content ratio (v/v) be
The vegetative grease of 5:10:5:5:5.
Comparative example 2, the grease for not preparing glyceride using microorganism and not fermenting (ratio is different from embodiment 1)
Preparation
Grape seed oil, wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil source oil content ratio (v/v) be
The vegetative grease of 20:20:20:20:20.
Comparative example 3 using microorganism does not prepare glyceride, (ratio the is same as Example 1) grease directly to ferment
Preparation
1) grape seed oil, wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil, content ratio (v/v) are 5:10:5:
5:5 is mixed according to a certain percentage；
2) tinder fungus (CGMCC 5.1494) is cultivated under aerobic condition, in culture solution to be proliferated；Fermentation can be
Under the conditions of 28 DEG C of temperature, fermentation in 72 hours is carried out, culture solution uses common production medium.
3) compound lard is added in the culture solution for completing culture tinder fungus proliferation, using compound lard as culture medium
Second of fermentation is carried out again.Wherein, second of fermentation can carry out fermentation in 72 hours under the conditions of 30 DEG C.Acid value is controlled 0.2
~0.5, terminate second and ferments.
4) grease after second fermenting is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 95 DEG C.
Comparative example 4 using microorganism does not prepare glyceride, is fermented with the different proportion vegetable oil with comparative example 3
Oil and fat preparation
1) grape seed oil, wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil, content ratio (v/v) are 5:10:
10:10:5 is mixed according to a certain percentage；
2) tinder fungus (CGMCC 5.1494) is cultivated under aerobic condition, in culture solution to be proliferated；Fermentation can be
Under the conditions of 28 DEG C of temperature, fermentation in 72 hours is carried out, culture solution uses common production medium.
3) compound lard is added in the culture solution for completing culture tinder fungus proliferation, using compound lard as culture medium
Second of fermentation is carried out again.Wherein, second of fermentation can carry out fermentation in 72 hours under the conditions of 30 DEG C.Acid value is controlled 0.2
~0.5, terminate second and ferments.
4) grease after second fermenting is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 95 DEG C.
Comparative example 5 prepares glyceride using microorganism, adds ratio vegetable oil same as Example 1, azymic preparation
Grease
1) to roll up branch Mucor fermentation medium as culture substrate, it is inoculated with Mucor circinelloides kind in the fermenter, inoculum concentration is
10%, fermentation temperature is at 30 DEG C, and fermentation time is in 72h；Stir 400r/min；pH 6.0；
2) it filters, obtains volume branch Mucor fungi wet cell, add with isopropanol quality (g) than 1:10 according to fungi quality (g)
Add isopropanol, impregnates 8h；It filters again, according to fungi quality (g) and 0.2mol/L, pH8.0 kaliumphosphate buffer quality (g) ratio
1:20 adds kaliumphosphate buffer, 28 DEG C of stirring 2h；It is separated by filtration, obtains upper layer oily liquids；
3) grease, grape seed oil obtained in step 2), wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil,
Content ratio is 70:5:10:5:5:5, is proportionally mixed；
4) grease is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 95 DEG C.
Comparative example 6 prepares glyceride, addition and 1 different proportion grease of embodiment, the oil of azymic preparation using microorganism
Rouge
1) to roll up branch Mucor fermentation medium as culture substrate, it is inoculated with Mucor circinelloides kind in the fermenter, inoculum concentration is
8%, fermentation temperature is at 30 DEG C, and fermentation time is in 72h；Stir 350r/min；pH 6.0；
2) it filters, obtains volume branch Mucor fungi wet cell, add with isopropanol quality (g) than 1:10 according to fungi quality (g)
Add isopropanol, impregnates 8h；It filters again, according to fungi quality (g) and 0.2mol/L, pH8.0 kaliumphosphate buffer quality (g) ratio
1:20 adds kaliumphosphate buffer, 28 DEG C of stirring 2h；It is separated by filtration, obtains upper layer oily liquids；
3) grease, grape seed oil obtained in step 2), wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil,
Content ratio is 40:5:10:10:5:10, is proportionally mixed；
4) grease is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 95 DEG C.
Comparative example 7 prepares glyceride, addition and 1 different proportion grease of embodiment, the grease for preparation of fermenting using microorganism
1) to roll up branch Mucor fermentation medium as culture substrate, it is inoculated with Mucor circinelloides kind in the fermenter, inoculum concentration is
8%, fermentation temperature is at 30 DEG C, and fermentation time is in 72h；Stir 350r/min；pH 6.0；
2) it filters, obtains volume branch Mucor fungi wet cell, add with isopropanol quality (g) than 1:10 according to fungi quality (g)
Add isopropanol, impregnates 8h；It filters again, according to fungi quality (g) and 0.2mol/L, pH8.0 kaliumphosphate buffer quality (g) ratio
1:20 adds kaliumphosphate buffer, 28 DEG C of stirring 2h；It is separated by filtration, obtains upper layer oily liquids；
3) grease, grape seed oil obtained in step 2), wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil,
Content ratio is 40:5:10:10:5:10, is proportionally mixed；
4) tinder fungus (CGMCC 5.1494) is cultivated under aerobic condition, in culture solution to be proliferated；Fermentation can be
Under the conditions of 28 DEG C of temperature, fermentation in 72 hours is carried out, culture solution uses common production medium；
5) compound lard is added in the culture solution for completing culture tinder fungus proliferation, using compound lard as culture medium
Third time fermentation is carried out again.Wherein, third time fermentation can carry out fermentation in 72 hours under the conditions of 30 DEG C.Acid value is controlled 0.2
~0.5, terminate third time and ferments.
6) grease after third time being fermented is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 95 DEG C.
Embodiment 6, toxicological test
The fermentation grease that the present invention obtains embodiment 1-5 carries out toxicological test, it is intended to its potential skin irritation into
Row assessment.
(1) multiple skin irritation test
Tested material preparation method: ferment oil prepared by embodiment 1-5
Animal subject: regular grade white New Zealand kind rabbit 4, weight 2.4-2.8kg,
Rearing conditions: 20-25 DEG C of temperature, relative humidity 40%-70%
Test method: for 24 hours shaving off back part of animal backbone diamond wool before test, each 3cm × 3cm of shaving range or so, examination
Tested material 0.5mL is uniformly applied on left side trial zone skin when testing, right side is compareed with distilled water.It smears daily once,
Continuous 14d, since second day, unhairing before smearing daily was cleaned with warm water, dermoreaction was observed after 1h and according to " cosmetics
Technical specification of security " pertinent regulations in (2015 editions) carry out skin wound repair scoring and calculate separately 14 days after the test
Total mark, 14 days every animal integral mean values, daily every animal integral mean value, and carry out skin strength classification.
Test result: tested material is in the multiple skin irritation test of rabbit, and every animal skin stimulate the reaction is long-pending daily
Dividing mean value is 0, is belonged to nonirritant.
(2) Skin allergy test
Tested material preparation method: ferment oil prepared by embodiment 1-5
Animal subject: Albino guinea pig is planted by regular grade Britain, is divided into 3 groups, tested material experimental group and positive substance experimental group,
Every group 20, tested material control group 10.Weight 223-293g.
Rearing conditions: 20-25 DEG C of temperature, relative humidity 40%-70%
Test method: for 24 hours by shaving on the left of guinea pig back, area about 6cm before test2.Induction exposure: by induction by
Examination object 0.2mL is coated on the left of tested material test group of animals on the skin in the region shaving 2cm × 2cm, with 2 layers of gauze, one layer of glass
6h is closed in paper covering, immobilization with adhesive tape.It is repeated once in the same way within 7th day and the 14th day.Positive substance experimental group is with 0.4%
2,4- dinitro-chloro-benzene acetone solns, tested material control group solvent make same processing.Excitation contact: after last induction
14 days, by excitation with tested material 0.2mL be coated on the right side of tested material experimental group and tested material control group before for 24 hours shaving 2cm ×
On the skin in the region 2cm, with 2 layers of gauze, 6h is closed in one layer of glassine paper covering, immobilization with adhesive tape, and positive substance experimental group is with 0.2%
2,4- dinitro-chloro-benzene acetone soln makees same excitation contact.24,48h observes dermoreaction after excitation contact, according to
Dermoreaction scoring and sensitization strength grading are carried out according to the pertinent regulations in " cosmetics safety technical specification " (2015 editions).
Test result: tested material is to guinea pig skin allergy test result, animal 0 in erythema oedema total score >=2,
Sensitization rate is 0%, has no skin allergic reaction.
(3) skin phototoxicity is tested
Tested material preparation method: ferment oil prepared by embodiment 1-5
Animal subject: regular grade Britain kind Albino guinea pig, every group 6, half male and half female, weight 245-281g.
Rearing conditions: 20-25 DEG C of temperature, relative humidity 40%-70%
Test method: surveying at back two by guinea pig back shaving for 24 hours before test and choose four pieces of skin, every piece of 2cm × 2cm,
Left side is 1,3 areas, and animal is fixed in right side 2,4th area, and in 1,2 area even application tested material 0.2mL, 3,4 areas are tested not apply
Object.After 30min, left side 1,3rd area are covered with aluminium foil, and adhesive tape is fixed, and right side is irradiated with UVA, sub-cage rearing.Respectively at irradiation
1,24,48h observation dermoreaction afterwards, and scored according to dermoreaction standards of grading, record the different observation period integrals of every animal
Value.Positive controls carry out in the same way, positive control 0.05%8- methoxyl group psoralea corylifolia.
Test result: tested material is to have no skin phototoxicity to guinea pig skin Phototoxicity experiment result.
The evaluation of experimental example 7, oxidation resistance
Using 1,1- diphenyl -2- trinitrophenyl-hydrazine (DPPH, 1,1-diphenyl 1-2-picry1hydrazy1) method with
The oil of comparative example 1 to comparative example 7 tests the anti-oxidant energy of the oil of embodiment 1 together.It is anti-oxidant to evaluate to measure freedom
1,1- diphenyl -2- trinitrophenyl-hydrazine (1,1-diphenyl 1-2- is utilized in base (free radical) elimination activity
picry1hydrazy1).That is, the 4.0 × 10 of 2.4mL-4In mol/L DPPH solution, respectively add 0.6mL embodiment 1-5,
After the oil of comparative example 1-7, places 30 minutes at normal temperature, measure absorbance, and at 517nm with the extinction for contrast groups
The reduction ratio of degree indicates antioxidant activity.
The evaluation of 1 oxidation resistance of table
| DPPH (%) | |
| Embodiment 1 | 88 |
| Embodiment 2 | 81 |
| Embodiment 3 | 83 |
| Embodiment 4 | 87 |
| Embodiment 5 | 85 |
| Comparative example 1 | 22 |
| Comparative example 2 | 25 |
| Comparative example 3 | 45 |
| Comparative example 4 | 48 |
| Comparative example 5 | 57 |
| Comparative example 6 | 46 |
| Comparative example 7 | 55 |
As a result as shown in Table 1 above, in the case where the ferment oil of oil composition preparation according to the present invention, with itself and comparison
The grease of example 1-7, which is compared, has anti-oxidant energy effect more outstanding.Comparative example 1,2,3,4 does not prepare glyceride, finds from a kind of table
Its oxidation resistance is far below embodiment 1- embodiment 5, and comparative example 5,6,7 is prepared for glyceride, but its oxidation resistance is still
It is old to be lower than embodiment 1-5.Especially comparative example 5 and comparative example 7 its oxidation resistance are better than other comparative examples, but significantly lower than real
Apply a 1-5.Wherein, comparative example 5 does not carry out subsequent fermentation and the grease ratio of 7 subsequent fermentation of comparative example is not advised in the present invention
In fixed range, illustrate that the preparation of fermentation grease has to pass through the grease ratio of subsequent fermentation and the fermentation of subsequent and vegetable oil
Also need present invention provide that in the range of can be only achieved antioxidant effect outstanding.
The skin sense sensory evaluation of experimental example 8, ferment oil
The skin sense sensory evaluation of ferment oil
Using 20 trained personnel of women (25-40 years old) as object, the skin skin sense sense organ of above-mentioned ferment oil is commented
The project of valence assess and find out it is average (using 1-5 as evaluation criteria, 1: it is excessively poor, 2: it is poor, 3: it is general, 4: well, 5:
It is outstanding).Its result is illustrated in following table 2.
The skin sense sensory evaluation of 2 ferment oil of table
| Evaluation score | |
| Embodiment 1 | 4.1 |
| Embodiment 2 | 4.0 |
| Embodiment 3 | 4.1 |
| Embodiment 4 | 4.0 |
| Embodiment 5 | 4.1 |
| Comparative example 1 | 1.8 |
| Comparative example 2 | 1.8 |
| Comparative example 3 | 2.9 |
| Comparative example 4 | 3.0 |
| Comparative example 5 | 2.1 |
| Comparative example 6 | 2.0 |
| Comparative example 7 | 3.3 |
As a result shown in table 2, the ferment oil of embodiment 1-5 is substantially better than comparative example 1-7 in skin skin sense evaluation.Comparative example
7 skin skin sense project appraisal is apparently higher than other comparative examples but significantly lower than embodiment 1-5.The results show that prepared by the present invention
Fermentation grease can sensory evaluation test in be better than comparative example 1-7, it is believed that fermentation grease prepared by the present invention has
Excellent skin sense.Comparative example 1,2,3,4 does not prepare glyceride, found from table 2 its skin sense lower than embodiment 1-5, comparative example 5,
6,7 it is prepared for glyceride, but its skin sense is still below embodiment 1-5.Wherein, comparative example 7, although its skin sense is right better than other
Ratio, but comparative example 1, skin sense are still lower than embodiment 1-5；The skin skin sense effect of comparative example 5 is better than other comparisons
The effect of example, but still not as good as embodiment 1-5, illustrate that the preparation of fermentation grease has to pass through the preparation of back glyceride, and
And the ratio of subsequent and vegetable oil fermentation also need present invention provide that in the range of can be only achieved skin sense effect outstanding.
Experimental example 9, value (TEWL) test of scattering and disappearing through transepidermal water
Human body is the highest skin barrier function evaluation method of more common and generally acknowledged degree through the lost value test of transepidermal water.Cause
This, the present embodiment evaluates skin barrier function using human trial.Specific test process is as follows:
1) the selection left and right inboard arm of subject is located away from palm base portion 5cm, in the identical area of left forearm 2 areas of definition
Domain (4cm × 4cm) is used as using area；The point of right forearm same position is as blank control region, the corresponding region of left and right arm
It tests simultaneously.
2) subject is cleaned with embodiment 1-7 respectively in two test areas of arm.1,2,4h after cleaning, using warp
Skin moisture loss measuring instrument Tewameter TM300 (German Courage Khazaka company) tests the water loss amount of skin,
Measurement 5 times, is averaged.
Blank control group is the average value of the 5 moisture loss values measured at the 0h moment.As a result as shown in table 3 below:
3 skin of table scatters and disappears test result through transepidermal water
By embodiment 1 in table 3 it is found that the ferment oil of embodiment 1-5 is tested in skin TEWL value compared with comparative example 1-7
In be better than comparative example 1-7, can be obviously improved using rear skin barrier function, reduce moisture of skin and scatter and disappear, there is good reparation
The function of skin barrier.The result shows that fermentation grease TEWL value prepared by the present invention is better than comparative example 1-7, illustrate system of the present invention
Standby fermentation grease has the function of excellent reparation skin barrier.Comparative example 1,2,3,4 does not prepare glyceride, sends out from table 2
Its existing skin barrier repair ability is lower than embodiment 1-5, and comparative example 5,6,7 is prepared for glyceride, but its skin barrier reparation
Ability is still below embodiment 1-5.
Experimental example 10, skin repair patch test
1, subjects
Suitable 10 people of volunteer is selected, the range of age was randomly choosed at 18-45 years old.
2, test method:
On volunteer's inner forearm healthy skin, is stimulated with the SDS (lauryl sodium sulfate) of appropriate concentration, be allowed to become
Then redness coats the present embodiment 1-5 sample and negative control (physiological saline) patch device respectively, light pressure makes sample liquid equably
It is pasted on damaged skin, observes the repairing effect of skin, as a result see the table below 4.
The skin repair patch test of 4 embodiment 1-5 of table
The experimental results showed that product of the present invention (embodiment 1-5) can effectively improve damaged skin appearance erythema, redness,
The symptoms such as decortication, can play the role of anti-inflammatory anti-itch, hence it is evident that improve skin repair ability.
Experimental example 11, oil oxidative stability test
120 DEG C of heating temperature, air mass flow 15.0L/h of setting, according to GB/T 21121-2007 " vegetable and animals oils lipid oxidation
The measurement (accelerated oxidation test) of stability " it is tested, test result is indicated with induction time h.
Stability test under the conditions of 5,120 DEG C of temperature of table
The results are shown in Table 5, and the comparative example oil oxidative stability for not preparing glyceride is lower than the comparison for preparing glyceride
Example and embodiment, the stability of the ferment oil of embodiment 1-5 are substantially better than comparative example 1-7.Comparative example 1,2,3,4 does not prepare glycerol
Ester finds its oil oxidative stability from 4 kinds of table lower than embodiment 1-5, and comparative example 5,6,7 is prepared for glyceride, but its oil
Lipid oxidation stability is still below embodiment 1-5, especially comparative example 5, although its oil oxidative stability is better than other comparisons
Example, but comparative example 1-5, oil oxidative stability are still lower than embodiment 1-5, and the preparation of the grease that illustrates to ferment must be through
Cross the preparation of back glyceride, and the ratio of subsequent and vegetable oil fermentation also need present invention provide that in the range of could
Reach oil oxidative stability outstanding.
Embodiment 12, dosage form citing
Dosage form example 1: cream
Firstly, moisturizer is added in water, heating is adjusted to 85 DEG C, is prepared water phase, is added oil-phase component respectively in oily phase,
85 DEG C are warming up to, oil is mutually added to water phase after mixing evenly, using blender homogeneous 20 minutes, has then carried out deaeration, mistake
Filter, cooling.
Table 6
Dosage form example 2: oils
Firstly, being gradually added into 3 kinds of feedstock oils in oily phase, stirring and being warming up to 85 DEG C, after keeping after twenty minutes gradually cooling
It is cooling, filtering and discharging.
Table 7
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited to above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring substantive content of the invention.
Claims (10)
1. a kind of fermentation grease, which is characterized in that the fermentation grease is to be prepared by a method comprising the following steps and obtain:
S1, to roll up branch Mucor fermentation medium as culture substrate, be inoculated with Mucor circinelloides kind；
S2, filtering obtain volume branch Mucor fungi wet cell, are that 1:8~10 adds isopropanol according to fungi and isopropanol mass ratio,
Impregnate 8~10h；It filters again, the mass ratio according to fungi and 0.2mol/L, pH8.0 kaliumphosphate buffer is that 1:15~20 add
Add the kaliumphosphate buffer, 28~32 DEG C of 1.5~2h of stirring；It is separated by filtration, obtains upper layer oily liquids；
Oily liquids, grape seed oil, wheat-germ oil, jojoba seed oil, camellia seed oil, avocado oil obtained in S3, step S2,
It is mixed in mass ratio for 70~75:5~10:5~10:5~10:5~10:5~10, obtains compound lard；
S4, under aerobic condition, in culture solution cultivate shelf fungus be proliferated；It is described culture be at 28~30 DEG C carry out 48~
Fermentation in 72 hours；
S5, the compound lard is added in the culture solution for completing culture shelf fungus proliferation, using the compound lard as culture
Base ferments again；
S6, the grease after step S5 fermentation is extracted using centrifuge separation, carries out squeezing recycling under the conditions of 95~96 DEG C to get institute
State fermentation grease.
2. fermentation grease as described in claim 1, which is characterized in that in step S1, inoculum concentration 8%-10%, fermentation temperature
At 28~30 DEG C, fermentation time stirs 300~400r/min, pH=6.0~6.5 in 60~84h.
3. as described in claim 1 fermentation grease, which is characterized in that in step S4, the shelf fungus be selected from tinder fungus,
One or more of heart rot, phelliuns igniarius, phellinus linteus, Phellinus pini.
4. as described in claim 1 fermentation grease, which is characterized in that in step S5, it is described fermentation be at 30~32 DEG C into
Row fermentation in 48~72 hours, acid value control is 0.2~0.5 in fermentation process.
5. a kind of fermentation grease as described in any one of claims 1 to 4 improves the use in skin sense function cosmetics in preparation
On the way.
6. a kind of fermentation grease as described in any one of claims 1 to 4 is preparing the purposes in anti-oxidation function cosmetics.
7. a kind of fermentation grease as described in any one of claims 1 to 4 has effects that oil oxidative stability in preparation
Purposes in cosmetics.
8. a kind of fermentation grease as described in any one of claims 1 to 4 is repaired in skin barrier function cosmetics in preparation
Purposes.
9. a kind of fermentation grease as described in any one of claims 1 to 4 is in preparation anti-inflammatory cosmetics, antipruritic cosmetics or skin
Purposes in skin repair function cosmetics.
10. the purposes as described in any one of claim 5~9, which is characterized in that the dosage form of the cosmetics is selected from skin toner
Water, lotion, massage cream, nourishing cream, hand lotion, soothing oil, essence, facial mask, washing cream, skin cream, shower cream.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111568814A (en) * | 2020-05-27 | 2020-08-25 | 广州市金因源生物技术有限公司 | Biological fermentation skin care emulsion and preparation method thereof |
| CN112641660A (en) * | 2021-01-12 | 2021-04-13 | 江苏瑞霆生物科技有限公司 | Preparation method of high-stability vegetable oil material fermentation self-packaging carrier, product and application thereof |
| CN115252478A (en) * | 2022-08-08 | 2022-11-01 | 北京茂思商贸有限公司 | Fermented vegetable oil and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111568814A (en) * | 2020-05-27 | 2020-08-25 | 广州市金因源生物技术有限公司 | Biological fermentation skin care emulsion and preparation method thereof |
| CN112641660A (en) * | 2021-01-12 | 2021-04-13 | 江苏瑞霆生物科技有限公司 | Preparation method of high-stability vegetable oil material fermentation self-packaging carrier, product and application thereof |
| CN112641660B (en) * | 2021-01-12 | 2021-10-22 | 江苏瑞霆生物科技有限公司 | Preparation method of high-stability vegetable oil material fermentation self-packaging carrier, product and application thereof |
| CN115252478A (en) * | 2022-08-08 | 2022-11-01 | 北京茂思商贸有限公司 | Fermented vegetable oil and preparation method and application thereof |
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